Preparation process of Chinese prescription compound plant fermentation stock solution

Abstract

The present invention discloses a Chinese prescription compound plant fermentation stock solution production process, and relates to the technical field of plant fermentation stock solution production, the Chinese prescription compound plant fermentation stock solution production process comprises the following steps: first, a plant raw material A and a plant raw material B are washed and dried; slicing and crushing the raw material A, and adding into a drying machine for heating and drying; carrying out supercritical extraction on the raw material A, and adding the raw material A into a fermentation tank; decocting the raw material B, extracting and concentrating the raw material B through an extracting and concentrating machine, and adding the concentrate into a fermentation tank; the invention has the following beneficial effects: traditional Chinese medicine components are converted from macromolecules to micromolecules through probiotics, and are completely absorbed and utilized by human body, so that the drug effect is improved, and the curative effect is effectively accelerated; the compound plants decompose toxicity in the fermentation process, toxic and side effects are reduced, and the taste of the traditional Chinese medicines can be improved.

Description

Technical Field

[0001] The invention relates to a fermentation stock solution production process, in particular to a Chinese medicine compound plant fermentation stock solution production process, belonging to the technical field of plant fermentation stock solution production. Background Art

[0002] Traditional Chinese medicine contains rich nutrients, which can provide the nutrients needed for microbial growth and reproduction. At the same time, traditional Chinese medicine can undergo drug activity transformation due to the growth and metabolism of microorganisms. The original structure can be transformed into new chemical components with stronger activity, lower toxicity and higher bioavailability. Microorganisms and traditional Chinese medicine fermentation interact with each other and work together to exert drug effects. Compared with traditional Chinese herbal medicine chemical and physical processing methods, Chinese herbal medicine fermentation can improve the utilization rate of certain ingredients that are difficult to be directly used, change drug activity, shorten drug action time, improve drug efficacy and reduce toxic side effects, and expand drug indications; After searching, a method for preparing a Chinese medicine fermentation broth is disclosed in application number CN202011297546.X, wherein the Chinese medicine fermentation broth is prepared by fermenting Chinese medicine extracts with fermentation bacteria, the Chinese medicine extracts are prepared by extracting Codonopsis pilosula, Astragalus membranaceus, Licorice, Isatis root, Honeysuckle, Tangerine peel and Hawthorn, and the fermentation bacteria are Enterococcus faecalis, Lactobacillus acidophilus and Lactobacillus plantarum, comprising the following steps: Step S1: statically expand and culture the fermentation bacteria in MRS liquid culture medium; Step S2: the expansion culture is divided into two stages and cultured for a period of time; Step S3: the Chinese medicine extracts are evenly mixed in proportion and added to the MRS liquid culture medium; Step S4: the culture medium is cooled after high-temperature sterilization; Step S5: inoculating the three strains expanded and cultured in step S2 into the cooled above-mentioned Chinese medicine culture medium; Step S6: after 48 hours of fermentation and mixed culture, the pH value is measured daily and the products are packaged. The present invention provides a method for preparing a Chinese medicine fermentation liquid which is not easy to mildew and improves the antioxidant property of water and the immunity of aquatic organisms; after further searching, a compound Chinese medicine fermentation liquid and a preparation method thereof disclosed in application number CN201910486185.4 are mainly composed of heart, liver, spleen, lung, kidney, qi and blood and auxiliary materials; the weight ratio of the components of the heart prescription medicinal materials is: 7 parts of Ligustrum lucidum, 9 parts of Schisandra chinensis, 9 parts of Radix Ophiopogonis, 5 parts of Coptis chinensis, 9 parts of Semen Ziziphi Spinosae, 9 parts of Astragalus membranaceus, 9 parts of Radix Angelicae Pubescentis, 9 parts, 9 parts of kudzu root, 9 parts of calamus and 2 parts of liquorice; the fungus medicine with medicinal fungi as the main body of the present invention performs solid fermentation on the prescription Chinese medicine to produce a variety of effective active ingredients and nutrients, realizes the extraction of full medicinal properties, and the medicinal efficacy is hundreds of times that of the original medicine. Compared with the traditional boiling and simmering extraction technology, the process preparation technology of the compound Chinese medicine fermentation liquid has the following characteristics: low-temperature solid extraction, maintaining the pure naturalness of the medicine, bionic multi-bacteria symbiosis, realizing full medicinal extraction, producing new active medicinal substances, degrading environmental pollutants, good taste, easy absorption and fast efficacy; However, the above method still has the following defects when used in practice: the decoctions or Chinese patent medicines taken by patients are not the final ingredients that directly enter the blood to exert the drug effect, but are only intermediate substances. After these intermediate substances enter the body, they must be digested and decomposed by the probiotics in the human gastrointestinal system before they can be converted into the final effective ingredients and enter the blood to exert the therapeutic effect of the drug. However, the probiotic flora of each person's gastrointestinal system is different. If the flora is unbalanced and the number of probiotics is small, the drug ingredients cannot be effectively decomposed and absorbed, and will be directly excreted from the body with feces, and the drug cannot play its due therapeutic role. Summary of the invention

[0003] The purpose of the present invention is to provide a process for producing a Chinese herbal compound plant fermentation stock solution, which has the advantages of improving the efficacy, accelerating the therapeutic effect, reducing non-toxic side effects, and solving the problems raised by the above-mentioned background technology.

[0004] To achieve the above object, the present invention provides the following technical solution: a process for preparing a Chinese herbal compound plant fermentation stock solution, comprising the following steps: Step 1: Cleaning and drying the plant raw material A and the plant raw material B; Step 2: Slice and crush the raw material A, and put it into a dryer for heating and drying; Step 3: subjecting raw material A to supercritical extraction and adding the raw material A to a fermentation tank; Step 4: decocting the raw material B, extracting and concentrating the raw material B through an extraction and concentrator, and then adding the concentrate to the fermentation tank; Step 5: Cultivate the starter and add it to the fermentation tank for primary fermentation; Step 6: Add a certain amount of diluent to the fermentation tank for secondary fermentation to obtain plant fermentation stock solution; Step 7: Sterilize the fermentation solution in packaging.

[0005] As a preferred technical solution of the present invention, the cleaning in step one is first performed by a brush cleaning machine for water spraying and brushing, and then disinfected and cleaned by an ozone bubbling cleaning machine.

[0006] As a preferred technical solution of the present invention, the slicing and crushing in step 2 utilizes multiple groups of cross-crushing blades to crush the cleaned raw materials into small particles of 10-30 mm.

[0007] As a preferred technical solution of the present invention, the supercritical extraction in step three is to pass the raw material A into an extraction tower, and add an extraction solvent to carry out an extraction reaction to obtain an extract.

[0008] As a preferred technical solution of the present invention, the extraction time in the extraction tower is 10-60 minutes, the extraction solvent is supercritical benzene, the extraction temperature in the extraction tower is 350-420° C., and the pressure is 9-11 MPa.

[0009] As a preferred technical solution of the present invention, in the starter culture in step five, bifidobacterium and lactic acid bacteria are separately inoculated into a culture medium, the culture medium is an acidic culture medium, and cultured at 28-40°C for 16 hours to obtain activated bifidobacterium and lactic acid bacteria species, and the active bifidobacterium and lactic acid bacteria species are placed in the culture solution according to a proportion, and the precipitate is centrifuged to obtain a strain cluster, which is added to the fermentation tank.

[0010] As a preferred technical solution of the present invention, bifidobacteria account for 0.6-2.2% and lactic acid bacteria account for 8-15% in the strain cluster.

[0011] Compared with the related art, the Chinese herbal compound plant fermentation stock solution production process provided by the present invention has the following beneficial effects: The ingredients of traditional Chinese medicine are transformed by probiotics from large molecules to small molecules, which are completely absorbed and utilized by the human body, thereby improving the efficacy and effectively speeding up the therapeutic effect. In addition, the compound plants decompose the toxicity during the fermentation process, reducing non-toxic side effects and improving the taste of traditional Chinese medicine. DETAILED DESCRIPTION

[0012] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0013] Example 1 The present invention provides a process for preparing a Chinese herbal compound plant fermentation stock solution, comprising the following steps: Step 1: Cleaning and drying the plant raw material A and the plant raw material B; Step 2: Slice and crush the raw material A, and put it into a dryer for heating and drying; Step 3: subjecting raw material A to supercritical extraction and adding the raw material A to a fermentation tank; Step 4: decocting the raw material B, extracting and concentrating the raw material B through an extraction and concentrator, and then adding the concentrate to the fermentation tank; Step 5: Cultivate the starter and add it to the fermentation tank for primary fermentation; Step 6: Add a certain amount of diluent to the fermentation tank for secondary fermentation to obtain plant fermentation stock solution; Step 7: Sterilize the fermentation solution in packaging.

[0014] Specifically, the cleaning in step one is first performed by a brush cleaning machine for water spraying and scrubbing, and then by an ozone bubbling cleaning machine for disinfection and cleaning.

[0015] Specifically, the slicing and crushing in step 2 utilizes multiple sets of cross-crushing blades to crush the cleaned raw materials into small particles of 10-30 mm.

[0016] Specifically, the supercritical extraction in step three is to pass the raw material A into the extraction tower, and add the extraction solvent to carry out the extraction reaction to obtain the extract, which is easy to separate from the raffinate phase and the extraction group after extraction.

[0017] Specifically, the extraction time in the extraction tower is 10 minutes, the extraction solvent is supercritical benzene, the extraction temperature in the extraction tower is 350° C., and the pressure is 9 MPa.

[0018] Specifically, in the starter culture in step five, bifidobacterium and lactic acid bacteria are separately inoculated into a culture medium, the culture medium is an acidic culture medium, and cultured at 28°C for 16 hours to obtain activated bifidobacterium and lactic acid bacteria species, and the active bifidobacterium and lactic acid bacteria species are placed in the culture solution according to a proportion, and the precipitate is centrifuged to obtain a strain cluster, which is added to a fermentation tank.

[0019] Specifically, bifidobacteria accounted for 0.6% and lactic acid bacteria accounted for 8% in the strain cluster.

[0020] Example 2 A process for preparing a Chinese herbal compound plant fermentation stock solution comprises the following steps: Step 1: Cleaning and drying the plant raw material A and the plant raw material B; Step 2: Slice and crush the raw material A, and put it into a dryer for heating and drying; Step 3: subjecting raw material A to supercritical extraction and adding the raw material A to a fermentation tank; Step 4: decocting the raw material B, extracting and concentrating the raw material B through an extraction and concentrator, and then adding the concentrate to the fermentation tank; Step 5: Cultivate the starter and add it to the fermentation tank for primary fermentation; Step 6: Add a certain amount of diluent to the fermentation tank for secondary fermentation to obtain plant fermentation stock solution; Step 7: Sterilize the fermentation solution in packaging.

[0021] Specifically, the cleaning in step one is first performed by a brush cleaning machine for water spraying and scrubbing, and then by an ozone bubbling cleaning machine for disinfection and cleaning.

[0022] Specifically, the slicing and crushing in step 2 utilizes multiple sets of cross-crushing blades to crush the cleaned raw materials into small particles of 10-30 mm.

[0023] Specifically, the supercritical extraction in step three is to pass the raw material A into the extraction tower, and add the extraction solvent to carry out the extraction reaction to obtain the extract, which is easy to separate from the raffinate phase and the extraction group after extraction.

[0024] Specifically, the extraction time in the extraction tower is 60 minutes, the extraction solvent is supercritical benzene, the extraction temperature in the extraction tower is 420° C., and the pressure is 11 MPa.

[0025] Specifically, in the starter culture in step five, bifidobacterium and lactic acid bacteria are separately inoculated into a culture medium, the culture medium is an acidic culture medium, and cultured at 0°C for 16 hours to obtain activated bifidobacterium and lactic acid bacteria species, and the active bifidobacterium and lactic acid bacteria species are placed in the culture solution according to a proportion, and the precipitate is centrifuged to obtain a strain cluster, which is added to a fermentation tank.

[0026] Specifically, bifidobacteria accounted for 2.2% and lactic acid bacteria accounted for 15% in the strain cluster.

[0027] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A process for producing a Chinese herbal compound plant fermentation stock solution, characterized in that: The following steps are involved: Step 1: Cleaning and drying the plant raw material A and the plant raw material B; Step 2: Slice and crush the raw material A, and put it into a dryer for heating and drying; Step 3: subjecting raw material A to supercritical extraction and adding the raw material A to a fermentation tank; Step 4: decocting the raw material B, extracting and concentrating the raw material B through an extraction and concentrator, and then adding the concentrate to the fermentation tank; Step 5: Cultivate the starter and add it to the fermentation tank for primary fermentation; Step 6: Add a certain amount of diluent to the fermentation tank for secondary fermentation to obtain plant fermentation stock solution; Step 7: Sterilize the fermentation solution in packaging.

2. The process for producing a Chinese herbal compound plant fermentation stock solution according to claim 1, characterized in that: The cleaning in step one is first performed by a brush cleaning machine for water spraying and scrubbing, and then disinfected and cleaned by an ozone bubbling cleaning machine.

3. The process for producing a Chinese herbal compound plant fermentation stock solution according to claim 1, characterized in that: The slicing and crushing in step 2 utilizes multiple sets of cross-crushing blades to crush the cleaned raw materials into small particles of 10-30 mm.

4. The process for producing a Chinese herbal compound plant fermentation stock solution according to claim 1, characterized in that: The supercritical extraction in step three is to pass the raw material A into the extraction tower, and add the extraction solvent to carry out the extraction reaction to obtain the extract.

5. The process for producing a Chinese herbal compound plant fermentation stock solution according to claim 4, characterized in that: The extraction time in the extraction tower is 10-60 minutes, the extraction solvent is supercritical benzene, the extraction temperature in the extraction tower is 350-420°C, and the pressure is 9-11MPa.

6. The process for producing a Chinese herbal compound plant fermentation stock solution according to claim 1, characterized in that: In the starter culture in step 5, bifidobacterium and lactic acid bacteria are separately inoculated into the culture medium, the culture medium is an acidic culture medium, and cultured at 28-40°C for 16 hours to obtain activated bifidobacterium and lactic acid bacteria species, and the active bifidobacterium and lactic acid bacteria species are placed in the culture solution according to a proportion, and the precipitate is centrifuged to obtain a strain cluster, which is added to the fermentation tank.

7. The process for producing a Chinese herbal compound plant fermentation stock solution according to claim 6, characterized in that: Bifidobacteria accounted for 0.6-2.2% of the strain cluster, and lactic acid bacteria accounted for 8-15%.