Biomimetic virus construction system based on DNA nanotechnology

Abstract

The invention discloses a bionic virus construction system based on a DNA nanotechnology. The bionic virus construction system comprises a virus information construction module, a virus information matching module, a bionic virus simulation construction module and a bionic virus construction module, a virus information construction module; a virus information matching module; the bionic virus simulation construction module is used for designing a polypeptide sequence according to the current virus key information in the virus information construction module and simulating and constructing a virus model; and the bionic virus construction module is used for assembling bionic viruses according to the virus model constructed in the virus simulation construction module. The invention belongs to the technical field of bionic virus construction, and achieves the technical effects that the information of the current virus and the information of the previous virus can be matched, screening is carried out after the similarity reaches the standard, a large amount of screening time can be shortened, a large amount of previous vaccine information can be integrated, a virus construction reference can be provided, and the screening efficiency is improved. And virtual simulation can be realized, so that the experiment time is shortened.

Description

Technical Field

[0001] The invention relates to the technical field of bionic virus construction, and in particular to a bionic virus construction system based on DNA nanotechnology. Background Art

[0002] Biomimetic virus construction refers to the design and manufacture of inactive virus-like particles with similar properties by simulating the structural characteristics and functional mechanisms of viruses in nature. These particles are usually made of biocompatible materials and can perform specific biological functions without causing infection.

[0003] DNA nanotechnology has experienced 40 years of vigorous development since its creation by Nadrian Seeman in 1982, and has been widely used in biology, medicine, materials, chemistry and other fields. The emergence of DNA origami technology in 2006 reduced the difficulty of designing DNA nanostructures and improved their programmability, thus greatly expanding the application scenarios of DNA nanotechnology. The bionic viruses constructed using DNA nanotechnology are very similar to real viruses, but they do not have the genetic material of viruses and cannot be replicated, so they can better achieve effective stimulation of the body's immune system.

[0004] Currently, the construction of bionic viruses is completed based on existing materials, and then implemented on experimental organisms to observe the effects. Finding matching bionic virus materials often requires consulting a large amount of information and data, which takes a lot of time and affects the matching efficiency. At the same time, specific application data can only be obtained after the experiment, resulting in a long construction cycle. Summary of the invention

[0005] To this end, the present invention provides a bionic virus construction system based on DNA nanotechnology to solve the above-mentioned problems in the prior art.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A bionic virus construction system based on DNA nanotechnology, comprising:

[0008] Virus information building module, virus information matching module, bionic virus simulation building module and bionic virus building module;

[0009] Virus information building module, obtains the current key virus information;

[0010] The virus information matching module matches the historical virus information in the module based on the current virus key information in the virus information construction module. When the matching similarity is greater than 80%, the historical virus information that meets the similarity standard is obtained, and the previous vaccine information is provided. At the same time, the comparison information of the current virus DNA model and the historical virus DNA model is extracted and constructed;

[0011] The bionic virus simulation building module designs the peptide sequence and simulates the construction of the virus model based on the current virus key information in the virus information building module;

[0012] The bionic virus building module assembles the bionic virus according to the virus model constructed in the virus simulation building module.

[0013] Furthermore, current key virus information includes the viral genome sequence, the characteristics of the structural proteins, and the mechanism of interaction between the virus and host cells.

[0014] Furthermore, the specific steps of simulating and constructing a virus model include:

[0015] S1: Carrier selection: According to the key information of the virus, select the corresponding nanocarrier to provide corresponding targeted delivery and functional enhancement;

[0016] S2: Framework nucleic acid construction: Using DNA origami technology, the staple chain is extended to the corresponding sequence at a specific position to construct the corresponding framework nucleic acid;

[0017] S3: Assemble virtual bionic cell components and select the same or similar DNA or RNA sequences based on previous vaccine information;

[0018] S4: Simulate the activities of virtual bionic cell components, and use known data to simulate the movement path and function of the current virtual bionic cell components.

[0019] Furthermore, nanocarriers include: organic nanocarriers, inorganic nanocarriers, and biomimetic carriers.

[0020] Furthermore, biomimetic carriers also include cell membrane nanovesicles:

[0021] Cell membrane nanovesicles include: natural cell or cell membrane-derived nanovesicles, exosomes, and small microvesicles.

[0022] Furthermore, the biomimetic carrier can also release submicron particles from cells in situ.

[0023] Furthermore, when the simulated activity of the virtual bionic cell element does not meet the expected effect, the process returns to S1 to reconstruct the virus.

[0024] Furthermore, the known data include current vector function data, current framework nucleic acid function data, and patient's in vivo biological environment data.

[0025] Furthermore, molecular walkers can be added to the bionic virus building modules.

[0026] The present invention has the following advantages:

[0027] The present invention can match the information of the current virus with the information of past viruses, and will only screen when the similarity meets the standard. This can reduce a lot of screening time, and can integrate a lot of past vaccine information to provide a reference for virus construction. It can also perform virtual simulation to reduce experimental time. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a module connection diagram of a bionic virus construction system based on DNA nanotechnology provided by the present invention.

[0029] Figure 2 A workflow diagram of a bionic virus simulation construction module of a bionic virus construction system based on DNA nanotechnology provided by the present invention. DETAILED DESCRIPTION

[0030] The following is a description of the implementation of the present invention by specific embodiments. People familiar with the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] like Figure 1 to Figure 2 As shown, the present invention provides a bionic virus construction system based on DNA nanotechnology, including: a virus information construction module, a virus information matching module, a bionic virus simulation construction module and a bionic virus construction module.

[0032] Virus information building module, which obtains the current key information of the virus, including the genome sequence of the virus, the characteristics of the structural proteins, and the mechanism of interaction between the virus and the host cell;

[0033] The virus information matching module matches the historical virus information in the module based on the current virus key information in the virus information construction module. When the matching similarity is greater than 80%, the historical virus information that meets the similarity standard is obtained, and the previous vaccine information is provided. At the same time, the comparison information of the current virus DNA model and the historical virus DNA model is extracted and constructed;

[0034] The bionic virus simulation building module designs the peptide sequence and simulates the construction of the virus model based on the current virus key information in the virus information building module, including:

[0035] S1: Carrier selection: According to the key information of the virus, select the corresponding nanocarrier to provide corresponding targeted delivery and functional enhancement. Nanocarriers include: organic nanocarriers, inorganic nanocarriers, and biomimetic carriers;

[0036] For example, lecithin-based nanoliposomes as organic nanocarriers can be delivered to Cas9 / sgRNA RNPs, which have the characteristics of high biocompatibility, low cytotoxicity, and high solution stability;

[0037] Gold nanoparticles, as inorganic nanocarriers, can be delivered to Cas9 / sgRNA RNP, DNA, and Cas9-sgRNA plasmids, and have unique optical and structural properties that can serve as a versatile platform for gene delivery;

[0038] Virus-like particles, as biomimetic nanocarriers, can be delivered to Cas9 / sgRNA RNP, achieving instantaneous and rapid delivery and reducing off-target rates;

[0039] Or choose cell membrane nanovesicles, genetically engineered cell membrane nanovesicles, etc., which show better targeting and immunotherapy capabilities, such as natural cell or cell membrane-derived nanovesicles, exosomes, small microvesicles, and submicron particles released by cells in situ;

[0040] S2: Framework nucleic acid construction: Using DNA origami technology, the corresponding sequence is extended from the staple chain at the specific position of the origami structure to construct the corresponding framework nucleic acid, which can realize the recognition and binding of target nucleic acid molecules (including DNA and RNA) in the solution through molecular hybridization, and construct its own nucleic acid properties, thereby providing conditions for the assembly of target molecule nucleic acid fragments. The structure of the framework nucleic acid includes but is not limited to triangles, rectangles, rods, nanotubes, nanocages, tetrahedrons, cubes, octahedrons, and icosahedrons;

[0041] S3: Assemble virtual bionic cell components and select the same or similar DNA or RNA sequences based on previous vaccine information;

[0042] S4: simulate the activity of virtual bionic cell components, use known data to simulate the movement path and function of the current virtual bionic cell components, and when the simulated activity of the virtual bionic cell components does not meet the expected effect, return to S1 to reconstruct the virus. The known data include the current vector function data, the current framework nucleic acid function data, and the biological environment data in the patient's body;

[0043] The bionic virus building module assembles the bionic virus according to the virus model constructed in the virus simulation building module.

[0044] A molecular walker can also be installed so that the bionic virus can move autonomously on the molecular track after the molecular walker is installed. The molecular walker is also constructed using DNA origami technology.

Claims

1. A bionic virus construction system based on DNA nanotechnology, characterized in that: include: Virus information building module, virus information matching module, bionic virus simulation building module and bionic virus building module; Virus information building module, obtains the current key virus information; The virus information matching module matches the historical virus information in the module based on the current virus key information in the virus information construction module. When the matching similarity is greater than 80%, the historical virus information that meets the similarity standard is obtained, and the previous vaccine information is provided. At the same time, the comparison information of the current virus DNA model and the historical virus DNA model is extracted and constructed; The bionic virus simulation building module designs the peptide sequence and simulates the construction of the virus model based on the current virus key information in the virus information building module; The bionic virus building module assembles the bionic virus according to the virus model constructed in the virus simulation building module.

2. The bionic virus construction system based on DNA nanotechnology according to claim 1, characterized in that: Current key virus information includes the viral genome sequence, the characteristics of its structural proteins, and the mechanism of interaction between the virus and host cells.

3. The bionic virus construction system based on DNA nanotechnology according to claim 1, characterized in that: The specific steps of simulating and building a virus model include: S1: Carrier selection: According to the key information of the virus, select the corresponding nanocarrier to provide corresponding targeted delivery and functional enhancement; S2: Framework nucleic acid construction: Using DNA origami technology, the staple chain is extended to the corresponding sequence at a specific position to construct the corresponding framework nucleic acid; S3: Assemble virtual bionic cell components and select the same or similar DNA or RNA sequences based on previous vaccine information; S4: Simulate the activities of virtual bionic cell components, and use known data to simulate the movement path and function of the current virtual bionic cell components.

4. The bionic virus construction system based on DNA nanotechnology according to claim 3 is characterized in that: Nanocarriers include: organic nanocarriers, inorganic nanocarriers, and biomimetic carriers.

5. The bionic virus construction system based on DNA nanotechnology according to claim 4, characterized in that: Biomimetic carriers also include cell membrane nanovesicles: Cell membrane nanovesicles include: natural cell or cell membrane-derived nanovesicles, exosomes, and small microvesicles.

6. The bionic virus construction system based on DNA nanotechnology according to claim 5, characterized in that: Biomimetic carriers can also release submicron particles from cells in situ.

7. The bionic virus construction system based on DNA nanotechnology according to claim 3 is characterized in that: When the simulated activity of the virtual bionic cell element does not meet the expected effect, it returns to S1 to reconstruct the virus.

8. The bionic virus construction system based on DNA nanotechnology according to claim 3 is characterized in that: The known data include current vector function data, current framework nucleic acid function data, and patient's in vivo biological environment data.

9. The bionic virus construction system based on DNA nanotechnology according to claim 1, characterized in that: Molecular walkers can also be added to the biomimetic virus building blocks.

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