Flooding-resistant identification box for monitoring hypoxia stress biomass inhibition in real time in rice bud stage

Abstract

The invention relates to the technical field of rice flood tolerance identification, and discloses a flood tolerance identification box for monitoring hypoxia stress biomass inhibition in real time in the rice bud stage, the flood tolerance identification box comprises a test box, the front side of the test box is provided with a support, the middle of the test box is fixedly provided with a support, and the top end of the support is movably provided with a floating rod; underwater rods are fixedly connected to the lower portions of the two ends of the floating rod, floating balls are fixedly installed at the ends of the two ends of the floating rod, and a lifting groove is formed in the upper side of the support. According to the invention, the underwater rod is arranged at the bottom of the floating rod, so that the effect of fixing the flooding depth of the flooding-resistant identification test is realized, and the situation that the setting of the test flooding depth is influenced by liquid level decline caused by absorption or natural evaporation of a culture solution is avoided; a bracket and a rotating shaft are arranged in the test box, so that the whole-plant growth ratio of the rice on the two sides of the floating rod in the period can be quickly obtained; and the length of the lifting groove formed in the bracket is gradually reduced in a gradient manner from front to back, so that a plurality of groups of experimental data can be conveniently observed and recorded.

Description

Technical Field

[0001] The invention relates to the technical field of rice flood tolerance identification test, in particular to a flood tolerance identification box for real-time monitoring of biomass inhibition caused by hypoxia stress during rice bud stage. Background Art

[0002] Rice is one of the world's important food crops, and half of the world's population eats rice. As the world's population gradually increases and the area of ​​high-quality arable land decreases, food security is an eternal issue facing breeders. About 30% of the world's rice farmland is located in low-altitude areas. Even if rice grows in a paddy field environment and can adapt well to a partially flooded environment, waterlogging can seriously affect the growth and yield of rice. Moreover, modern high-yield rice varieties cannot survive in extremely flooded conditions of complete submergence. Therefore, cultivating flood-resistant rice suitable for growing in low-altitude areas is of great significance to ensuring food security.

[0003] However, in the existing rice flooding tolerance test, the identification equipment in the identification laboratory is simple, and the test personnel need to replenish water regularly during the cultivation process to maintain the flooding state set in different experiments, and the test errors caused by human factors are relatively large; when measuring the growth of rice seedlings under hypoxia stress, since the roots of rice seedlings are very thin and very easy to break, the operation of taking out the seedlings to measure the growth has a greater impact on the root system, especially for rice that has encountered flooding hypoxia stress and has poor growth conditions. The rice seedlings are often measured and then abandoned to avoid the impact of measurement damage on the experimental results. Real-time non-destructive detection of the same plant at different growth stages cannot be achieved. Therefore, a flooding tolerance identification device that can monitor the biomass inhibition of rice seedlings in real time is needed. Summary of the invention

[0004] The invention aims at the shortcomings of the existing rice flood tolerance identification box mentioned in the background technology during use.

[0005] The present invention provides the following technical solution: a flood resistance identification box for real-time monitoring of biomass inhibition caused by low oxygen stress during rice bud stage, comprising a test box, a front side of the test box is provided, a bracket is fixedly installed in the middle of the test box, a floating rod is movably installed on the top of the bracket, underwater rods are fixedly connected below both ends of the floating rod, floating balls are fixedly installed at the end positions of both ends of the floating rod, a lifting groove is opened on the upper side of the bracket, a rotating shaft is movably sleeved in the middle of the lifting groove, a rotating groove is opened in the middle of the floating rod, a pointer is fixedly connected to the front side of the rotating shaft, and dials are fixedly connected to the positions on both sides of the bracket near the bottom of the bracket.

[0006] Preferably, the two ends of the floating rod are symmetrically distributed, and a culture port is opened in the middle of both ends of the floating rod and the underwater rod, and a culture net is fixedly installed in the middle of the culture port.

[0007] Preferably, the length of the lifting slots provided on each group of the brackets decreases gradually from front to back.

[0008] Preferably, the underwater rod and the floating rod are movably connected via a screw rod, and a scale is set on the screw rod.

[0009] The present invention has the following beneficial effects: 1. The present invention achieves the effect of fixing the flooding depth of the flooding resistance identification test by arranging an underwater rod at the bottom of the floating rod, thereby preventing the culture solution from being absorbed or naturally evaporated to cause the liquid level to drop and affect the setting of the test flooding depth; by arranging a bracket and a rotating shaft in the test box, it is possible to quickly obtain the whole plant growth ratio of rice on both sides of the floating rod during this period; by arranging the length of the lifting groove on the bracket to decrease gradually from front to back, it is convenient to observe and record multiple groups of experimental data.

[0010] 2. The present invention achieves the effect of fixing the flooding depth of the flooding resistance identification test by arranging an underwater rod at the bottom of the floating rod, thereby preventing the culture solution from being absorbed or naturally evaporated, causing the liquid level to drop and affecting the setting of the test flooding depth. The fixed underwater rod is replaced with a movable one that can adjust the distance from the bottom surface of the remaining floating rods, thereby realizing free regulation of the flooding depth of the flooding resistance test.

[0011] 3. The present invention arranges a bracket and a rotating shaft in the test box to serve as the central fulcrum of the floating rod, so that when the floating rod is not affected by buoyancy after the culture solution in the test box is discharged, the floating rod can rotate around the rotating shaft under the support of the bracket, thereby quickly obtaining the whole plant growth ratio of rice on both sides of the floating rod during this period. In this process, the root system of the rice plant will not be damaged, and its subsequent cultivation and detection will not be affected.

[0012] 4. The present invention arranges the length of the lifting groove on the bracket to decrease gradually from the front to the back, and installs it on the front of the test box, so that when the rice flooding resistance test is carried out on multiple groups of floating rods, the different inclination angles of the multiple groups of floating rods and the ratios indicated by the pointers can be observed from the front, making it easy to observe and record. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the cross-sectional structure of the present invention; Figure 3 It is a schematic diagram of the measurement state structure of the present invention.

[0014] In the figure: 1. test box; 12. drain outlet; 2. bracket; 3. lifting slot; 4. rotating shaft; 41. pointer; 5. floating rod; 51. culture port; 52. culture net; 53. rotating slot; 54. underwater rod; 6. dial. DETAILED DESCRIPTION

[0015] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0016] See also Figure 1 A flood tolerance identification box for real-time monitoring of biomass inhibition caused by hypoxia stress during rice bud stage, comprising a test box 1, a bracket 2 is fixedly installed in the middle of the test box 1, a floating rod 5 is movably installed on the top of the bracket 2, and underwater rods 54 are fixedly connected to the lower ends of the floating rod 5. The two sides of the floating rod 5 are symmetrically distributed, and a culture port 51 is opened in the middle of the floating rod 5 and the ends of the underwater rod 54. A culture net 52 is fixedly installed in the middle of the culture port 51, and the culture net 52 can be freely disassembled from the culture port 51. Rice seeds are placed on the culture net 52 for experimental culture. The ends of the floating rod 5 are located at the ends of the floating rod 5. A float is fixedly installed to assist the float rod 5 to float on the water surface in the test box 1. At this time, the underwater rod 54 is in an underwater submerged position. The cultivation nets 52 on both sides of the float rod 5 are respectively installed in the cultivation port 51 at the float rod 5 and the cultivation port 51 at the underwater rod 54, so that the seeds on both sides of the float rod 5 can be cultivated under different flooding depths, thereby comparing the growth of the seeds under different flooding conditions, monitoring the degree of inhibition of the biomass under hypoxia stress, and fixing the flooding depth of the flooding group at the same time to prevent the culture solution from being absorbed or naturally evaporated, causing the liquid level to drop and affecting the setting of the test flooding depth.

[0017] See also Figure 2-3A lifting groove 3 is provided on the upper side of the bracket 2, and a rotating shaft 4 is movably sleeved in the middle of the lifting groove 3. A rotating groove 53 is provided in the middle of the floating rod 5, and both ends of the rotating shaft 4 are fixedly connected to the middle position of the rotating groove 53, so that the floating rod 5 can move up and down along the lifting groove 3 with the rotating shaft 4. When the culture solution in the test box 1 is discharged, the liquid level in the test box 1 drops, and the floating rod 5 stops falling when the liquid level drops to the bottom of the lifting groove 3 when the rotating shaft 4 moves to the bottom of the lifting groove 3. At this time, the floating rod 5 is far away from the water surface and is no longer supported by buoyancy. It only tilts to the heavier side of the two sides under the action of gravity. A pointer 41 is fixedly connected to the front of the rotating shaft 4, and a dial 6 is fixedly connected to the positions on both sides of the bracket 2 near the bottom of the bracket 2. When the floating rod 5 is only affected by gravity, When tilted, the pointer 41 is driven to rotate, and a weight ratio scale is engraved on the front of the dial 6, so that the ratio of the whole plant growth of rice on both sides of the floating rod 5 during this period can be quickly obtained. The length of the lifting slot 3 opened on each group of brackets 2 decreases gradually from front to back. The front of the test box 1 is provided with 11, which is convenient for observing the different tilt angles of multiple groups of floating rods 5 and the ratio indicated by the pointer 41 from the front. After recording, 11 is closed and the culture solution is injected into the test box 1 again, so that the floating rod 5 rises with the liquid level due to the buoyancy, thereby avoiding the problem of plant root damage caused by taking it out to measure the growth during the growth stage of the rice seedlings, and realizing non-destructive measurement of the ratio of flooded and normal plant growth, without affecting its subsequent continued cultivation and detection.

[0018] In addition, the underwater rod 54 and the floating rod 5 can be movably connected by a screw, and a scale is set on the screw to achieve the regulation of the distance between the underwater rod 54 and the floating rod 5, so that the flooding depth of the rice planted in the cultivation net 52 on the underwater rod 54 can be freely adjusted, making it suitable for the setting of different experimental flooding depths.

[0019] The working principle of the method of the present invention is as follows: When in use, adjust the height of the underwater rod 54 from the floating rod 5 to make it consistent with the test flooding depth, install the cultivation net 52 in the cultivation port 51 of the floating rod 5 at both ends of the floating rod 5 and the cultivation port 51 of the underwater rod 54 respectively, and place the test rice seeds on the cultivation nets 52 at the two locations respectively, and inject the culture solution into the test box 1. As the culture solution is injected, the floating rod 5 floats up driven by the float balls on both sides, driving the rotating shaft 4 to move up along the lifting groove 3. In this process, the floating rod 5 is always flush with the liquid surface, ensuring that the two groups of seeds at the floating rod 5 on both sides of the bracket 2 and the underwater rod 54, one group is at the liquid level and grows normally, and the other group is located below the liquid surface at the set flooding depth position, until monitoring is required. When measuring the total growth ratio of two groups of rice seeds, when opening 11 to discharge the culture solution in the test box 1, the liquid level in the test box 1 drops, and the float rod 5 drops with the liquid level until the rotating shaft 4 moves to the bottom of the lifting tank 3 and stops falling. At this time, the float rod 5 is far away from the water surface and is no longer supported by buoyancy. It only tilts toward the heavier side of the two sides under the action of gravity. At this time, the ratio indicated by the pointer 41 on the dial 6 is recorded. During the measurement process, there is no need to pull the rice roots out of the cultivation net 52, thereby avoiding measurement damage to the rice root system during the measurement process. After the culture solution is injected into the test box 1 again, the experimental rice of this group can continue to be cultivated, thereby realizing real-time monitoring of the biomass inhibition of the same plant in the rice bud stage.

[0020] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0021] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A flood tolerance identification box for real-time monitoring of biomass inhibition caused by hypoxia stress during rice bud stage, comprising a test box (1), characterized in that: The front of the test box (1) is provided with (11), a bracket (2) is fixedly installed in the middle of the test box (1), a floating rod (5) is movably installed on the top of the bracket (2), underwater rods (54) are fixedly connected below the two ends of the floating rod (5), and floating balls are fixedly installed at the ends of the two ends of the floating rod (5), a lifting groove (3) is opened on the upper side of the bracket (2), a rotating shaft (4) is movably sleeved in the middle of the lifting groove (3), a rotating groove (53) is opened in the middle of the floating rod (5), a pointer (41) is fixedly connected to the front of the rotating shaft (4), and dials (6) are fixedly connected to the positions near the bottom of the bracket (2) on both sides of the bracket (2).

2. A flood tolerance identification box for real-time monitoring of biomass inhibition caused by hypoxia stress during rice budding period according to claim 1, characterized in that: The two ends of the floating rod (5) are symmetrically distributed, and a culture port (51) is provided in the middle of both ends of the floating rod (5) and the underwater rod (54), and a culture net (52) is fixedly installed in the middle of the culture port (51).

3. The flood tolerance identification box for real-time monitoring of biomass inhibition caused by hypoxia stress during rice budding period according to claim 1, characterized in that: The length of the lifting slots (3) provided on each group of the brackets (2) decreases gradually from the front to the back.

4. The flood tolerance identification box for real-time monitoring of biomass inhibition caused by hypoxia stress during rice budding period according to claim 1, characterized in that: The underwater rod (54) and the floating rod (5) are movably connected via a screw rod, and a scale is provided on the screw rod.

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