Functional eye-protecting soft sweets capable of resisting oxidation and reducing apoptosis of eye cells and preparation method of functional eye-protecting soft sweets

Abstract

The invention discloses functional eye-protecting soft sweets capable of resisting oxidation and reducing apoptosis of eye cells and a preparation method. The functional eye-protecting soft sweets comprise the following components in percentage by weight: 2-10% of functional components and the balance of auxiliary materials, the functional components comprise at least one of blueberry anthocyanin, lutein ester, zeaxanthin, astaxanthin, lycopene and beta-carotene, as well as vitamin C and / or vitamin E. The functional soft sweets provided by the invention have the effects of removing DPPH free radicals and reducing eye apoptotic cells, and compared with soft sweets without vitamin C / vitamin E, the vitamin C / vitamin E added in the formula can reduce the attenuation degree of functional nutrients in the effective period.

Description

Technical Field

[0001] The invention relates to the field of food technology, and in particular to a functional eye-protecting soft candy capable of resisting oxidation and reducing apoptosis of eye cells and a preparation method thereof. Background Art

[0002] Eyes are windows to the soul, and eye health management is an important matter. Myopia, astigmatism, glaucoma, macular degeneration, hyperopia, fundus lesions, cataracts and other eye problems are relatively common in modern society, which will have more adverse effects on people's lives and reduce the quality of life. Instrument treatment, medication, and eye drops are common treatments for eye problems. However, this method is inconsistent with the modern people's pursuit of a healthy life. There are more and more health products or foods for eye health. However, the products currently on the market for eye health have the following shortcomings:

[0003] 1) Currently, there are relatively few types of anti-oxidant and anti-apoptosis ocular cell gummies on the market, the combinations are relatively simple, there are fewer antioxidant nutrients, and the functions are not comprehensive enough.

[0004] 2) The antioxidant functional raw materials in the product often have poor stability and are easily degraded during the shelf life.

[0005] 3) Currently, most of the antioxidant functional raw materials on the market are capsules. With the increasing demand for antioxidants and eye health among more young people, there is a need for an eye health nutritional supplement that is more nutritious and interesting for young people.

[0006] In summary, a convenient and delicious antioxidant and eye health gummy candy has great market prospects. Summary of the invention

[0007] In order to overcome the deficiencies of the prior art, one of the purposes of the present invention is to provide a functional soft candy that can resist oxidation and reduce apoptosis of eye cells.

[0008] The second object of the present invention is to provide a method for preparing functional soft candy that can resist oxidation and reduce apoptosis of eye cells.

[0009] One of the purposes of the present invention is achieved by the following technical solution:

[0010] A functional soft candy for resisting oxidation and reducing eye cell apoptosis comprises the following components in percentage by weight: 2-10% of functional ingredients and the balance of auxiliary materials; the functional ingredients include at least one of blueberry anthocyanins, lutein esters, zeaxanthin, astaxanthin, lycopene and beta-carotene, and vitamin C and / or vitamin E.

[0011] Blueberry anthocyanins are rich in anthocyanins and anthocyanidins. As a natural antioxidant from blueberries, they have antioxidant activity that can scavenge free radicals, increase antioxidant enzyme activity, and inhibit lipid peroxidation, and can be used to prevent oxidative stress damage to the eye.

[0012] Lutein esters have strong free radical scavenging ability and antioxidant activity, thereby alleviating inflammatory responses and reducing oxidative damage to eye tissue cells.

[0013] There are 11 conjugated double bonds in the molecular structure of zeaxanthin, forming a large conjugated system. The presence of these conjugated double bonds enables zeaxanthin to block the chain transfer of free radicals, thus having strong antioxidant activity. It is an important strong antioxidant that can be used by the human body, and plays an antioxidant role by reducing the reactivity of chemically active substances such as free radical singlet oxygen and photochemical sensitizers.

[0014] Astaxanthin is a carotenoid with strong antioxidant properties. Its chemical structure is a six-membered ring structure consisting of four isoprene units connected in the form of conjugated double bonds, with two isoprene units at both ends. These structures can effectively quench highly oxidative singlet reactive oxygen species and other free radicals in the environment, thereby scavenging free radicals and having an antioxidant effect.

[0015] Lycopene is a carotenoid found in plant foods. It has 11 conjugated double bonds and 2 non-conjugated double bonds in its molecular structure, so it has strong antioxidant function.

[0016] β-carotene is an antioxidant that has been found to have the ability to quench singlet oxygen.

[0017] Vitamin C and vitamin E are both antioxidants that protect nutrients from decaying during their shelf life.

[0018] As a preferred embodiment of the present invention, the lutein ester is provided in the form of lutein ester microcapsule powder; the zeaxanthin is provided in the form of (3R, 3'R)-dihydroxy-β-carotene powder; and the astaxanthin is provided in the form of Haematococcus pluvialis.

[0019] As a preferred embodiment of the present invention, the functional ingredients include lutein esters, and at least one of blueberry anthocyanins, zeaxanthin, astaxanthin, lycopene and β-carotene; and also include vitamin C and / or vitamin E.

[0020] As a preferred embodiment of the present invention, the functional ingredients include lutein ester, zeaxanthin, vitamin C and vitamin E.

[0021] As a preferred embodiment of the present invention, the functional ingredients include lutein ester, zeaxanthin, blueberry anthocyanin, astaxanthin, lycopene, vitamin C and vitamin E.

[0022] As a preferred embodiment of the present invention, the functional soft candy for anti-oxidation and reducing eye cell apoptosis comprises the following components by weight percentage: 2-10% functional ingredient, 70-90% maltitol liquid, 5-7% gelatin, 2-6% erythritol, 0.5-5% α-cyclodextrin, and 0.1-0.25% agar.

[0023] As a preferred embodiment of the present invention, the functional soft candy for resisting oxidation and reducing eye cell apoptosis further comprises: 0.2%-1.5% of sour agent and 0.1%-0.25% of compound coating agent.

[0024] As a preferred embodiment of the present invention, the acidulant includes citric acid and / or DL-malic acid.

[0025] As a preferred embodiment of the present invention, the compound coating agent includes caprylic acid glyceride and / or carnauba wax. Coating the surface of the soft candy with the compound coating agent, such as caprylic acid glyceride and carnauba wax, can reduce the contact of the soft candy with oxygen and reduce the attenuation of antioxidant nutrients during the effective period.

[0026] As a preferred embodiment of the present invention, the functional soft candy for anti-oxidation and reducing eye cell apoptosis includes the following components in weight percentage: 3% lutein ester microcapsule powder, 0.3% zeaxanthin powder, 0.25% vitamin C, 0.25% vitamin E, 81.6% maltitol liquid, 6% gelatin, 4% erythritol, 3% α-cyclodextrin, 0.3% agar, 0.8% citric acid, 0.4% DL-malic acid, 0.05% caprylic decanoate, and 0.05% carnauba wax.

[0027] As a preferred embodiment of the present invention, the functional soft candy for anti-oxidation and reducing eye cell apoptosis comprises the following components in weight percentage: 2% lutein ester microcapsule powder, 0.2% zeaxanthin powder, 0.3% blueberry anthocyanin, 0.4% Haematococcus pluvialis, 0.4% lycopene, 0.25% vitamin C, 0.25% vitamin E, 81.6% maltitol liquid, 6% gelatin, 4% erythritol, 3% α-cyclodextrin, 0.3% agar, 0.8% citric acid, 0.4% DL-malic acid, 0.05% caprylic decanoate, and 0.05% carnauba wax.

[0028] The second object of the present invention is achieved by adopting the following technical solution:

[0029] A method for preparing functional soft candy for anti-oxidation and reducing eye cell apoptosis as described in any one of the purposes, comprising the following steps:

[0030] Gelatinization: Mix and dissolve the formulated amount of agar with purified water to obtain liquid 1; Mix and dissolve the formulated amount of gelatin with purified water to obtain liquid 2;

[0031] Dissolving sugar: put liquid 1 into a boiling tank, add maltitol liquid of one-fourth of the formula amount and erythritol of the formula amount, boil at 125±5℃ to obtain liquid 3;

[0032] Preparation: dissolving and mixing one-half of the formula amount of maltitol liquid, water-soluble functional raw materials and purified water to obtain water suspension liquid 4; mixing oil-soluble functional raw materials, the remaining maltitol liquid, the formula amount of α-cyclodextrin and purified water to obtain oil suspension liquid 5; mixing the formula amount of acidulant and purified water to obtain flavor liquid 6; the water-soluble functional raw materials include blueberry anthocyanins and / or vitamin C; the oil-soluble functional raw materials include lutein esters, zeaxanthin and vitamin E, astaxanthin and / or lycopene;

[0033] Mixing: Evenly mix the feed liquid 2, the feed liquid 3, the feed liquid 4, the feed liquid 5 and the feed liquid 6 to obtain the mixed feed liquid 7;

[0034] Casting and molding: pouring the mixed liquid into the mold to shape and dry it to obtain a semi-finished soft candy;

[0035] After demoulding, the composite coating agent is sprayed on the surface of the semi-finished soft candy to obtain the soft candy.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] (1) The functional soft candy for anti-oxidation and reducing ocular cell apoptosis provided by the present invention has the function of scavenging DPPH free radicals and reducing ocular apoptotic cells. Moreover, compared with the soft candy without adding vitamin C / vitamin E, adding vitamin C / vitamin E in the formula can reduce the attenuation degree of functional nutrients during the shelf life.

[0038] (2) The method for preparing functional soft candies for anti-oxidation and reducing ocular cell apoptosis provided by the present invention can reduce the attenuation of antioxidant nutrients in the soft candies during their shelf life by adding vitamin C and / or vitamin E; coating the surface of the soft candies with a composite coating agent can reduce the contact of the soft candies with oxygen and reduce the attenuation of antioxidant nutrients during their shelf life. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 A schematic diagram of the zebrafish eye tissue structure provided by an embodiment of the present invention;

[0040] Figure 2 This is a graph showing the results of exploring the effect of functional soft candies on reducing ocular cell apoptosis provided by an embodiment of the present invention.

[0041] In the picture: 1. Zebrafish eye; 2. Retina. DETAILED DESCRIPTION

[0042] The present invention is further described below in conjunction with the accompanying drawings and specific embodiments. It should be noted that, under the premise of no conflict, the embodiments or technical features described below can be arbitrarily combined to form new embodiments. The raw materials, equipment, etc. used in the following embodiments can be obtained by purchase unless otherwise specified.

[0043] Comparative Examples 1-2 and Examples 1-9

[0044] A functional soft candy is prepared from functional ingredients and auxiliary materials, wherein the functional ingredients include blueberry anthocyanin, lutein ester (derived from lutein ester microcapsule powder), zeaxanthin (derived from zeaxanthin powder), astaxanthin (derived from Haematococcus pluvialis), lycopene, vitamin C and vitamin E; the auxiliary materials include maltitol, gelatin, erythritol, α-cyclodextrin, acidulant, agar, and compound coating agent. The compound coating agent is prepared from caprylic decanoic acid glyceride and carnauba wax in a ratio of 1:1. The specific formula is shown in Table 1 below.

[0045] Table 1 Formula of Comparative Examples 1-2 and Examples 1-9 Soft Candies

[0046]

[0047] The soft candies of Comparative Examples 1-2 and Examples 1-9 were prepared according to the following method:

[0048] 1. Gelatinization: Mix and dissolve the formulated amount of agar with purified water to obtain liquid 1; mix and dissolve the formulated amount of gelatin with purified water to obtain liquid 2;

[0049] 2. Dissolving sugar: put liquid 1 into a boiling tank, add maltitol solution of one-fourth of the formula amount and erythritol of the formula amount, boil at 125±5℃ to the target concentration, and obtain liquid 3;

[0050] 3. Preparation: Dissolve and mix 1 / 2 of the formula amount of maltitol liquid, water-soluble functional raw materials (blueberry anthocyanins, vitamin C) and purified water to obtain water suspension liquid 4; mix the oil-soluble functional raw materials (lutein ester microcapsule powder, zeaxanthin powder, Haematococcus pluvialis, lycopene, and vitamin E), the remaining maltitol liquid, the formula amount of α-cyclodextrin and purified water to obtain oil suspension liquid 5; mix the acidulant and purified water to obtain flavor liquid 6;

[0051] 4. Mixing: Evenly mix feed liquid 2, feed liquid 3, feed liquid 4, feed liquid 5 and feed liquid 6 to obtain mixed feed liquid 7;

[0052] 5. Casting and molding: pour the mixed liquid into the starch mold to shape and dry;

[0053] 6. After demoulding, spray the obtained soft candy surface evenly with the compound coating agent.

[0054] Performance Testing

[0055] 1. Explore the protective effect of vitamin C on the attenuation of functional nutrients during their shelf life

[0056] The antioxidant functional nutrients in the soft candies of Example 1, Example 2, Example 6 and Example 7 are lutein ester (derived from lutein ester microcapsule powder) and zeaxanthin (derived from zeaxanthin powder). The soft candies of Example 1, Example 2, Example 6 and Example 7 are placed in accelerated experimental conditions of 37±2°C, RH75±5% and long-term experimental conditions of 25°C±2°C, RH60±5%, and the attenuation of lutein ester and zeaxanthin under the experimental conditions is observed.

[0057] Table 1 Stability of Example 1 and Example 2 under accelerated test conditions

[0058]

[0059]

[0060] Table 2 Stability of Example 1 and Example 2 under long-term experimental conditions

[0061]

[0062] As shown in Table 1-2, by comparing the experimental results of Example 1 and Example 2, it can be found that under the protection of vitamin C, the decay rate of lutein ester and zeaxanthin in Example 2 is lower. By adding vitamin C to the soft candy formula, the decay degree of other antioxidant functional ingredients during the effective period can be slowed down, and at the same time, the amount of expensive functional raw materials can be reduced, which is conducive to reducing production costs.

[0063] Similarly, by comparing the results of Example 1 and Example 6, it can be found that under the protection of vitamin E, the attenuation rate of lutein ester and zeaxanthin in Example 6 is lower.

[0064] Comparison of the results of Example 2, Example 6 and Example 7 shows that the use of vitamin C and vitamin E as protective agents can better protect the functional ingredients.

[0065] 2. Explore the effect of functional soft candy on scavenging DPPH free radicals

[0066] 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) is a nitrogen-based free radical with asymmetric valence electrons on the nitrogen-nitrogen bond, which is easy to undergo electron or proton transfer reactions with compounds with electron or proton donors. When a free radical scavenger is present, it will undergo an electron pairing reaction with DPPH, reducing the blue color of DPPH, and its absorbance liquid is negatively correlated with antioxidants. The absorbance of the sample is measured at 517nm using an enzyme reader.

[0067] The calculation formula of DPPH free radical scavenging rate of the sample is as follows:

[0068] Clearance rate (%) = (A control –A sample ) / A control ×100%

[0069] Where: A control A is the absorbance of DPPH solution without sample added (replaced by ethanol solution of the same volume) at 517 nm; sample is the absorbance of the DPPH solution to which the sample was added at 517 nm.

[0070] The samples of Comparative Examples 1-2 and Examples 1-9 were prepared into 100 μg / mL working solution with 95% ethanol and used immediately. 0.1 ml of sample was mixed with 0.3 ml of 0.3 mmol / L DPPH solution and reacted at room temperature in the dark for 30 min. The statistical results were expressed as mean ± SE. SPSS 26.0 software was used for statistical analysis, and p < 0.05 indicated that the difference was statistically significant.

[0071] Table 3 Experimental results of functional soft candy on the evaluation of scavenging DPPH free radical function (n=10)

[0072] Group DPPH free radical scavenging rate (%) Comparative Example 1 2.13±0.36 Comparative Example 2 70.17±5.60** Example 1 76.44±7.58** Example 2 90.39±8.07*** Example 3 84.70±5.79*** Example 4 86.29±8.47*** Example 5 12.11±0.35 Example 6 88.35±5.47*** Example 7 91.33±6.41*** Example 8 95.35±5.31*** Example 9 87.34±6.06***

[0073] Note: In the table, compared with comparative example 1, **p<0.01, ***p<0.001

[0074] As shown in Table 3, compared with Comparative Example 1, the functional soft candies of Examples 1-4 and 6-9 all have significant DPPH radical scavenging rates, indicating that the composite composition of lutein ester and zeaxanthin, blueberry anthocyanins, astaxanthin and lycopene can eliminate free radicals, which helps to reduce oxidative damage to the eye. Compared with Comparative Example 2, the functional soft candies of Examples 1-4 and 6-9 all have more significant antioxidant effects, and the results show that lutein ester is compounded with a very small amount of other eye protection functional ingredients (zeaxanthin, blueberry anthocyanins, astaxanthin and lycopene), which can more significantly inhibit the generation of free radicals, and are conducive to protecting the health of the eyes. The comparison of Example 1, Example 5 and Example 2 shows that the addition of vitamin C can improve the overall scavenging DPPH radical effect of the soft candy product, and the combination of vitamin C, lutein and zeaxanthin has a synergistic effect in scavenging DPPH radicals.

[0075] The functional ingredients of Example 8 include lutein ester, zeaxanthin, blueberry anthocyanins, astaxanthin, lycopene, vitamin C and vitamin E, and its effect of scavenging DPPH free radicals is the best, which is better than Example 2 using lutein ester, zeaxanthin and vitamin C as functional ingredients. Although the functional ingredients of Example 9 also include lutein ester, zeaxanthin, blueberry anthocyanins, astaxanthin, lycopene, vitamin C and vitamin E, the dosage of these functional ingredients is changed compared with Example 8, and the effect of the soft candy of Example 9 on scavenging DPPH free radicals is greatly reduced compared with Example 8, and is even worse than that of Example 2. It can be seen that the soft candy of the embodiment of the present invention uses lutein ester, zeaxanthin, blueberry anthocyanins, astaxanthin, lycopene, vitamin C and vitamin E as functional ingredients, and it is crucial to reasonably control the dosage of each functional ingredient.

[0076] 3. Explore the effect of functional soft candy on reducing apoptotic cells in the eye

[0077] 1dpf wild-type AB strain zebrafish were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well. Comparative Examples 1-2 and Examples 2-9 were prepared into 250μg / mL working solutions with ultrapure water and used immediately. The above working solutions were given in water, and a blank control group was set up at the same time, with a capacity of 3mL per well. Except for the blank control group, the remaining experimental groups were given water-soluble mycophenolic acid morpholine ethyl ester to establish a zebrafish visual fatigue model. After 1 day of treatment at 28°C, AO staining was performed. After staining, 10 zebrafish were randomly selected from each experimental group and placed under a fluorescence microscope for photography. NIS-Elements D 3.20 advanced image processing software was used to analyze and collect data, and the fluorescence intensity of apoptotic cells in the eyes was analyzed. The statistical processing results are expressed as mean±SE. SPSS26.0 software was used for statistical analysis, and p<0.05 indicated that the difference was statistically significant.

[0078] Table 4 Experimental results of functional soft candy in reducing ocular apoptotic cells (n=10)

[0079] Group Fluorescence intensity of apoptotic cells in the eye (pixels, mean ± Blank control 179087S±E7)833**** Comparative Example 1 354801±22548 Comparative Example 2 314172±9356* Example 1 301627±10453* Example 2 251582±17982*** Example 3 251155±20514*** Example 4 261990±28207*** Example 5 334853±20511 Example 6 257588±17953*** Example 7 250121±19513*** Example 8 236581±17256*** Example 9 267542±21952***

[0080] Note: In the table, compared with comparative example 1, *p<0.05, ***p<0.001, ****p<0.0001

[0081] As shown in Table 4, compared with Comparative Example 1, under the same visual fatigue model, the functional soft candies of Examples 2-4 and Examples 6-7 can reduce the severity of apoptosis of eye cells, and have the effect of inhibiting apoptotic cells in the eye and protecting eye health. Compared with Comparative Example 2, the functional soft candies of Examples 2-4 can more significantly reduce the severity of apoptosis of eye cells. The above data can show that compared with a single lutein ester component, lutein ester compounded with a very small amount of other eye protection functional components (zeaxanthin, blueberry anthocyanins, astaxanthin and lycopene) can more significantly reduce the number of apoptotic cells in the eye, and have a more effective effect of inhibiting apoptotic cells in the eye and protecting eye health.

[0082] Comparison of Example 1, Example 2 and Example 5 shows that the addition of vitamin C can improve the overall effect of the soft candy product in reducing apoptotic cells in the eye, and the combination of vitamin C, lutein and zeaxanthin has a synergistic effect in reducing apoptotic cells in the eye.

[0083] The effective ingredients of Example 8 include lutein ester, zeaxanthin, blueberry anthocyanin, astaxanthin, lycopene, vitamin C and vitamin E, and its effect of reducing ocular apoptotic cells is the best, which is better than Example 2 using lutein ester, zeaxanthin and vitamin C as effective ingredients. Although the effective ingredients of Example 9 also include lutein ester, zeaxanthin, blueberry anthocyanin, astaxanthin, lycopene, vitamin C and vitamin E, the dosage of these effective ingredients is changed compared with Example 8, and the effect of the soft candy of Example 9 on reducing ocular apoptotic cells is greatly reduced compared with Example 8, and even worse than the effect of Example 2. It can be seen that the soft candy of the embodiment of the present invention uses lutein ester, zeaxanthin, blueberry anthocyanin, astaxanthin, lycopene, vitamin C and vitamin E as effective ingredients, and the reasonable control of the dosage of each effective ingredient is directly related to the effect of reducing ocular apoptotic cells.

[0084] 4. Histological analysis of functional soft candy in reducing ocular cell apoptosis

[0085] 1dpf wild-type AB strain zebrafish were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well. The Example 2 group was given 62.5μg / mL, 125μg / mL, and 250μg / mL of water-soluble solution, respectively, and the positive control glutathione was 615μg / mL. A blank control group and a comparative example 1 group (250μg / mL) were set up at the same time, and the capacity of each well was 3mL. Except for the blank control group, the remaining experimental groups were all given water-soluble mycophenolic acid morpholine ethyl ester to establish a zebrafish visual fatigue model. After 1 day of treatment at 28°C, the zebrafish was fixed with 4% tissue cell fixative, and after a series of dehydration-embedding-sectioning-staining and other steps, the zebrafish was subjected to histopathological H&E staining analysis. The effect of eye protection soft candy on the ocular tissue structure of zebrafish was evaluated by ocular tissue pathology analysis.

[0086] like Figure 1 The figure shows a schematic diagram of the zebrafish eye tissue structure. In the figure, the red frame line is the analysis site zebrafish eye 1, and the enlarged image is a display of the retina 2.

[0087] Figure 2 In the figure, A is a blank control, B is a comparative example 1 group; C is a glutathione 615 μg / mL group; D1 is a 62.5 μg / mL group of Example 2, D2 is a 125 μg / mL group of Example 2, and D3 is a 250 μg / mL group of Example 2.

[0088] like Figure 2 As shown, the ocular histological examination of zebrafish in comparative example 1 showed widespread apoptosis of retinal cells, manifested as cell shrinkage and chromatin marginalization. The ocular histological examination of zebrafish in the positive control glutathione treatment group showed that the apoptosis of retinal cells in the eye was significantly improved, indicating that glutathione has a significant improvement effect on apoptosis of zebrafish ocular cells.

[0089] like Figure 2 As shown, in the 62.5 and 125 μg / mL concentration groups of Example 2, retinal cells generally underwent apoptosis, indicating that the above concentration groups had no significant effect on improving cell apoptosis. The ocular pathological histological examination of zebrafish in the 250 μg / mL concentration group of Example 2 showed that retinal cell apoptosis was improved, indicating that this concentration group had the effect of improving eye cell apoptosis.

[0090] In summary, under the experimental conditions, the functional soft candy of Example 2 has the effect of relieving visual fatigue, which is specifically manifested in the improvement of cell apoptosis.

[0091] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A functional soft candy for anti-oxidation and reducing ocular cell apoptosis, characterized in that: The invention comprises the following components in percentage by weight: 2-10% of functional ingredients and the balance of auxiliary materials; the functional ingredients comprise at least one of blueberry anthocyanins, lutein esters, zeaxanthin, astaxanthin, lycopene and beta-carotene, as well as vitamin C and / or vitamin E.

2. The functional soft candy for anti-oxidation and reducing ocular cell apoptosis according to claim 1, characterized in that: The lutein ester is provided in the form of lutein ester microcapsule powder; the zeaxanthin is provided in the form of (3R, 3'R)-dihydroxy-β-carotene powder; and the astaxanthin is provided in the form of Haematococcus pluvialis.

3. The functional soft candy for anti-oxidation and reducing eye cell apoptosis according to claim 1, characterized in that: The functional ingredients include lutein esters, and at least one of blueberry anthocyanins, zeaxanthin, astaxanthin, lycopene and beta-carotene; and also include vitamin C and / or vitamin E.

4. The functional soft candy for anti-oxidation and reducing ocular cell apoptosis according to claim 1, characterized in that: The functional ingredients include lutein ester, zeaxanthin, vitamin C and vitamin E; Alternatively, the functional ingredients include lutein esters, zeaxanthin, blueberry anthocyanins, astaxanthin, lycopene, vitamin C and vitamin E.

5. The functional soft candy for anti-oxidation and reducing ocular cell apoptosis according to any one of claims 1 to 4, characterized in that: The invention comprises the following components in percentage by weight: 2-10% of functional ingredients, 70-90% of maltitol liquid, 5-7% of gelatin, 2-6% of erythritol, 0.5-5% of alpha-cyclodextrin and 0.1-0.25% of agar.

6. The functional soft candy for anti-oxidation and reducing ocular cell apoptosis according to claim 5, characterized in that: Also includes: Acidulant 0.2%-1.5%, compound coating agent 0.1-0.25%.

7. The functional soft candy for anti-oxidation and reducing eye cell apoptosis according to claim 6, characterized in that: The acidulant includes citric acid and / or DL-malic acid; the compound coating agent includes caprylic and capric glyceride and / or carnauba wax.

8. The functional soft candy for anti-oxidation and reducing ocular cell apoptosis according to claim 7, characterized in that: The invention comprises the following components in percentage by weight: 3% lutein ester microcapsule powder, 0.3% (3R, 3'R)-dihydroxy-β-carrot powder, 0.25% vitamin C, 0.25% vitamin E, 81.6% maltitol liquid, 6% gelatin, 4% erythritol, 3% α-cyclodextrin, 0.3% agar, 0.8% citric acid, 0.4% DL-malic acid, 0.05% caprylic decanoate and 0.05% carnauba wax.

9. The functional soft candy for anti-oxidation and reducing ocular cell apoptosis according to claim 7, characterized in that: The invention comprises the following components in percentage by weight: 2% of lutein ester microcapsule powder, 0.2% of (3R, 3'R)-dihydroxy-β-carrot powder, 0.3% of blueberry anthocyanin, 0.6% of Haematococcus pluvialis, 0.5% of lycopene, 0.25% of vitamin C, 0.25% of vitamin E, 81.6% of maltitol liquid, 6% of gelatin, 4% of erythritol, 3% of α-cyclodextrin, 0.3% of agar, 0.8% of citric acid, 0.4% of DL-malic acid, 0.05% of caprylic decanoate and 0.05% of carnauba wax.

10. A method for preparing the functional soft candy for anti-oxidation and reducing eye cell apoptosis according to any one of claims 6 to 9, characterized in that: The following steps are involved: Gelatinization: Mix and dissolve the agar in the formula amount with purified water to obtain liquid 1; Mix and dissolve the gelatin in the formula amount with purified water to obtain liquid 2; Dissolving sugar: put liquid 1 into a boiling tank, add maltitol liquid of one-fourth of the formula amount and erythritol of the formula amount, boil at 125±5℃ to obtain liquid 3; Preparation: dissolve and mix one-half of the formula amount of maltitol liquid, water-soluble functional raw materials and purified water to obtain water suspension liquid 4; mix the oil-soluble functional raw materials, the remaining maltitol liquid, the formula amount of α-cyclodextrin and purified water to obtain oil suspension liquid 5; mix the formula amount of acidulant and purified water to obtain flavor liquid 6; the water-soluble functional raw materials include blueberry anthocyanins and / or vitamin C; the oil-soluble functional raw materials include lutein ester microcapsule powder, zeaxanthin powder and vitamin E, as well as Haematococcus pluvialis and / or lycopene; Mixing: Evenly mix the feed liquid 2, the feed liquid 3, the feed liquid 4, the feed liquid 5 and the feed liquid 6 to obtain the mixed feed liquid 7; Casting and molding: pouring the mixed liquid into the mold to shape and dry it to obtain a semi-finished soft candy; After demoulding, the composite coating agent is sprayed on the surface of the semi-finished soft candy to obtain the soft candy.

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