Dietary fiber composition for improving immunity and intestinal health and application thereof

Abstract

The invention discloses a dietary fiber composition for improving immunity and intestinal health and application thereof, and belongs to the technical field of functional foods.The dietary fiber composition is composed of asparagus powder, licorice powder, loquat powder, avocado powder and sea buckthorn powder, and the weight ratio of the loquat powder to the avocado powder to the sea buckthorn powder is (1-10): (1-10): (0.5-3). The dietary fiber composition composed of the asparagus powder, the licorice powder, the loquat powder, the avocado powder and the sea buckthorn powder has the effects of enhancing immunity, regulating intestinal flora functions and relaxing bowel. Experiments find that when the weight ratio of the loquat powder to the avocado powder to the sea buckthorn powder is (1-10): (1-10): (0.5-3), the dietary fiber composition has a synergistic effect in the aspect of enhancing immunity.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, and in particular to a dietary fiber composition for improving immunity and intestinal health and an application thereof. Background Art

[0002] Immunity refers to the sum of biological effects produced by the body in the process of identifying and responding to "antigenic foreign bodies" or "foreign components". Under normal circumstances, it is a specific physiological reaction to maintain the stability of the internal environment, and immunity is a defense mechanism of the body itself. Age, physical fitness and living habits are three factors that affect immunity. In addition, seasonal changes, unbalanced nutrition, irregular lifestyle, excessive fatigue and stress can all affect the function of the human immune system.

[0003] About 70% of the human immune system exists in the intestines, and a large number of immune cells such as T cells, B cells, and NK cells are concentrated in the intestinal mucosa. The intestine is the first line of defense for immunity. As a place for nutrient digestion and absorption, microbial colonization, and immune cell aggregation, the intestine promotes the interaction between the three. Normal intestinal flora plays an important role in promoting the development of the immune system, maintaining normal immune function, and synergistically antagonizing the invasion of pathogens. Therefore, when the intestinal flora is disordered, the body's resistance is likely to deteriorate.

[0004] CN114982891A discloses a method for preparing a fruit and vegetable enzyme stock solution beverage, which is prepared by fermentation using asparagus, loquat, licorice and the like as raw materials. The invention can make human cells more active, significantly accelerate metabolism, and improve human immunity, but does not disclose its effect on improving intestinal health. CN102138594A discloses a compound seabuckthorn tea preparation with the function of regulating intestinal flora. The compound seabuckthorn tea preparation includes, by weight percentage: oligofructose 15%-90%, oligomaltose 0%-40%, oligogalactose 0%-30%, seabuckthorn fruit powder 1%-60%, black tea powder 1%-60%, and auxiliary materials 0%-50%. The invention has the health care function of regulating intestinal flora and is suitable for people with intestinal dysfunction, but does not disclose its effect on enhancing immunity.

[0005] In summary, the functions of functional foods for enhancing immunity and regulating intestinal function are relatively single, and the two cannot be taken into account at the same time. Therefore, it is very necessary to find a dietary fiber composition that can enhance immunity and intestinal health at the same time. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides a dietary fiber composition for improving immunity and intestinal health and its application.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions: In a first aspect, the present invention provides a dietary fiber composition for improving immunity and intestinal health, which is composed of asparagus powder, licorice powder, loquat powder, avocado powder and sea buckthorn powder.

[0008] Asparagus is rich in nutrients such as amino acids, various minerals and vitamins. It also contains various biologically active ingredients such as polysaccharides, saponins and flavonoids. It has the effects of promoting the proliferation and differentiation of immune cells, regulating the function of immune cells and moisturizing the intestines and promoting bowel movements.

[0009] Licorice contains a variety of biologically active ingredients such as polysaccharides, glycyrrhizic acid, triterpenoids, flavonoids, etc., which can maintain the normal structure and function of cells, regulate the activity and number of immune cells, reduce intestinal inflammation, promote the repair and regeneration of intestinal mucosa, inhibit the growth of harmful intestinal bacteria, and promote the balance of intestinal flora.

[0010] Loquat can improve the phagocytic and bactericidal abilities of immune cells, reduce cell apoptosis, promote intestinal peristalsis, improve the intestinal environment, reduce the accumulation of harmful substances in the intestine, and reduce the risk of intestinal diseases.

[0011] Avocado, rich in unsaturated fatty acids and bioactive ingredients such as plant sterols, tocopherol, squalene, etc., can enhance the activity of immune cells, support cell repair and regeneration, protect immune cells from oxidative damage, protect the intestinal barrier and promote the growth of intestinal probiotics.

[0012] Sea buckthorn can reduce cell damage, enhance humoral immunity and cellular immunity, and at the same time improve the intestinal microecological structure and maintain the balance of intestinal flora.

[0013] Experiments have shown that the invention has the effects of enhancing immunity, regulating intestinal flora function and moistening the intestine and facilitating bowel movements by compounding asparagus powder, licorice powder, loquat powder, avocado powder and sea buckthorn powder.

[0014] In some embodiments, the weight ratio of loquat powder, avocado powder and sea buckthorn powder is 1-10:1-10:0.5-3.

[0015] In some embodiments, the dietary fiber composition comprises the following ingredients by weight: 5-15 parts of asparagus powder, 3-20 parts of licorice powder, 1-10 parts of loquat powder, 1-10 parts of avocado powder and 0.5-3 parts of sea buckthorn powder.

[0016] Preferably, the dietary fiber composition comprises the following raw materials by weight: 5-12 parts of asparagus powder, 5-15 parts of licorice powder, 1-5 parts of loquat powder, 3-8 parts of avocado powder and 1-2 parts of sea buckthorn powder.

[0017] Further preferably, the dietary fiber composition comprises the following raw materials by weight: 10 parts of asparagus powder, 8 parts of licorice powder, 3 parts of loquat powder, 5 parts of avocado powder and 1 part of sea buckthorn powder.

[0018] In some embodiments, the weight ratio of loquat powder, avocado powder and sea buckthorn powder is 1-5:3-8:1-2.

[0019] Preferably, the weight ratio of the loquat powder, avocado powder and sea buckthorn powder is 3:5:1.

[0020] In a second aspect, the present invention provides a method for preparing the dietary fiber composition for improving immunity and intestinal health, comprising the following steps: Mix the prescribed amounts of asparagus powder, licorice powder, loquat powder, avocado powder and sea buckthorn powder and sift.

[0021] In some embodiments, the sieving is through a 60-80 mesh sieve.

[0022] In a third aspect, the present invention provides use of the dietary fiber composition for improving immunity and intestinal health in the preparation of functional foods.

[0023] In some embodiments, the function is at least one of enhancing immunity, regulating intestinal flora function, and moisturizing the intestines and promoting bowel movements.

[0024] In a fourth aspect, the present invention provides a composition for enhancing immunity and intestinal health, comprising the above-mentioned dietary fiber composition and an auxiliary material acceptable in functional foods.

[0025] In some embodiments, the composition of the present invention is in the form of granules, powder, blocks or pastes.

[0026] The beneficial effects of the present invention are: (1) Experiments have shown that the dietary fiber composition of the present invention, which is composed of asparagus powder, licorice powder, loquat powder, avocado powder and sea buckthorn powder, has the effects of enhancing immunity, regulating intestinal flora function and moisturizing the intestine and facilitating bowel movements.

[0027] (2) The experiment found that when the weight ratio of loquat powder, avocado powder and sea buckthorn powder was 1-10:1-10:0.5-3, the dietary fiber composition produced a synergistic effect in enhancing immunity.

[0028] (3) The dietary fiber composition of the present invention has clear ingredients, good safety, and a simple preparation method, and is suitable for large-scale promotion and application.

[0029] (4) The functional food prepared using the dietary fiber of the present invention can effectively enhance immunity and intestinal health, has good safety, and can be used for a long time. DETAILED DESCRIPTION

[0030] The following description of the embodiments is only used to help understand the method of the present invention and its core idea. It should be noted that for those of ordinary skill in the art, without departing from the principles of the present invention, several improvements and modifications may be made to the present invention, and these improvements and modifications also fall within the scope of protection of the claims of the present invention. The following description of the disclosed embodiments enables professionals and technicians in this field to implement or use the present invention. The various modifications to these embodiments will be apparent to professionals and technicians in this field, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but may be applied to a wider range consistent with the principles and novel features disclosed herein.

[0031] When the embodiment gives a numerical range, it should be understood that, unless otherwise specified in the present invention, the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those of ordinary skill in the art to which the present invention belongs.

[0032] It is worth noting that the raw material information used in the present invention is shown in Table 1 below.

[0033] Table 1 Raw material information

[0034] Dietary fiber compositions of Examples 1-5 and Comparative Examples 1-4 The dietary fiber compositions of different embodiments and comparative examples were prepared according to Table 2 and the following preparation methods.

[0035] Preparation method: Mix the formulated amounts of asparagus powder, licorice powder, loquat powder, avocado powder and sea buckthorn powder, and pass through an 80-mesh sieve.

[0036] Table 2 Dietary fiber composition formula of the embodiment and comparative example (unit: weight portion)

[0037] Remark: 1.“-” in the table means not added.

[0038] 2. The “*” in the table represents the use of white kidney bean powder instead of asparagus powder, wherein the white kidney bean powder is made by grinding white kidney beans.

[0039] 3. The preparation method of Comparative Example 1 in the table is: mix the asparagus powder, licorice powder, loquat powder and avocado powder in the formula amount, and pass through an 80-mesh sieve.

[0040] 4. The preparation method of Comparative Example 2 in the table is as follows: Mix the asparagus powder, licorice powder and sea buckthorn powder in the formula amount and pass through an 80-mesh sieve.

[0041] 5. The preparation method of Comparative Example 4 in the table is as follows: white kidney bean powder, licorice powder, loquat powder, avocado powder and sea buckthorn powder in the formula amount are mixed and passed through an 80-mesh sieve.

[0042] Test Example 1: Evaluation of the efficacy of dietary fiber composition in enhancing immunity Experiments were conducted in accordance with the requirements of the "Health Food Function Test and Evaluation Methods (2022 Edition)" to study the efficacy of dietary fiber compositions in enhancing immunity.

[0043] 1. Experimental Animals SPF grade ICR mice, weighing 18-20g.

[0044] 2. Test samples Dietary fiber compositions prepared in Examples 1-5 and Comparative Examples 1-4.

[0045] 3. Animal grouping ICR mice were randomly divided into a normal group, Example 1-5 groups and Comparative Example 1-4 groups according to body weight, with 8 mice in each group. Example 1-5 groups and Comparative Example 1-4 groups were gavaged with the dietary fiber composition prepared in the corresponding examples or comparative examples at a dose of 50 mg / kg, and the normal group was gavaged with an equal volume of physiological saline once a day for 30 consecutive days. All groups had normal diet and drinking water.

[0046] After the last oral administration, the mice were fasted for 12 h, then killed, and the lymphocyte proliferation capacity and NK cell activity were measured.

[0047] 4. Experimental methods (1) Determination of ConA-induced mouse spleen lymphocyte proliferation ability The mice were killed, the spleen was removed aseptically, ground, sieved, and spleen cell suspension was prepared. The cell number was adjusted to 3×10 6 Each spleen cell suspension was added to two wells of a 24-well culture plate, 1 mL per well, and 75 μL ConA solution (equivalent to 7.5 μg mL -1 ), and the other well was used as a control and cultured in a 5% CO2, 37°C CO2 incubator for 72 h. 4 h before the end of the culture, 0.7 mL of supernatant was gently aspirated from each well, and 0.7 mL of RPMI1640 culture medium without calf serum was added, and MTT (5 mg mL -1) 50 μL / well, continue to culture for 4 h. After the culture is completed, add 1 mL of acidic isopropanol to each well, blow and mix to completely dissolve the purple crystals. Then dispense into 96-well culture plates, make 3 parallel wells in each well, and use a microplate reader to measure the optical density value at a wavelength of 570 nm.

[0048] The proliferation capacity of lymphocytes was represented by the optical density value of the wells with ConA added minus the optical density value of the wells without ConA added. The results are shown in Table 3.

[0049] Table 3 Effect of dietary fiber composition on ConA-induced proliferation of mouse spleen lymphocytes

[0050] Note: Different letters in the table indicate significant differences among the groups, P < 0.05.

[0051] The results show: Compared with the normal group, the proliferation ability of lymphocytes in the Example 1-5 group was significantly improved (P<0.05), indicating that the dietary fiber composition provided by the Example 1-5 has the ability to improve the proliferation of mouse spleen lymphocytes.

[0052] By comparing comparative example groups 1-2 and example group 1, it can be seen that in the technical scheme claimed to be protected by the present invention, the dietary fiber composition of loquat powder and avocado powder or sea buckthorn powder mixed with asparagus powder and licorice powder alone has a poor ability to enhance the proliferation of spleen lymphocytes, or even has no significant effect, while the dietary fiber composition of loquat powder, avocado powder and sea buckthorn powder mixed with asparagus powder and licorice powder has a significantly improved lymphocyte proliferation ability (P<0.05), producing a synergistic effect.

[0053] (2) NK cell activity assay 24 h before the experiment, the target cells (YAC-1 cells) were subcultured and the cell concentration was adjusted to 4×10 5 The mice were killed, the spleens were removed aseptically, and spleen cell suspension was prepared as effector cells. The cell concentration was adjusted to 2×10 7 100 μL of target cells and effector cells (50:1 effector-target ratio) were added to a U-shaped 96-well culture plate; 100 μL of target cells and culture medium were added to the target cell natural release well, and 100 μL of target cells and 1% NP40 were added to the target cell maximum release well; three parallel wells were set for each of the above items, and cultured in a 37°C, 5% CO2 incubator for 4 h, and then the 96-well culture plate was rotated at 1500 r·min -1 Centrifuge for 5 min, aspirate 100 μL of supernatant from each well and place it in a flat-bottom 96-well culture plate. Add 100 μL of LDH matrix solution and react for 3-10 min at room temperature. Add 1 mol·L-1 Add 30 μL of HCl and measure the optical density (OD) at 490 nm using a microplate reader.

[0054] The NK cell activity was calculated according to the following formula. The results are shown in Table 4.

[0055] NK cell activity (%) =

[0056] Table 4 Effects of dietary fiber composition on NK cell activity in mice

[0057] Note: Different letters in the table indicate significant differences among the groups, P < 0.05.

[0058] The results show: Compared with the normal group, the NK cell activity of the mice in Example 1-5 groups was significantly increased (P<0.05), indicating that the dietary fiber composition prepared in Example 1-5 has the ability to enhance the activity of NK cells.

[0059] In summary, the dietary fiber compositions prepared in Examples 1-5 of the present invention can improve the proliferation ability of lymphocytes, enhance the activity of NK cells, and have the effect of enhancing immunity.

[0060] Test Example 2: Evaluation of the efficacy of dietary fiber composition in regulating intestinal flora function According to the requirements of the "Health Food Function Test and Evaluation Methods (2022 Edition)", experiments were conducted to study the efficacy of dietary fiber compositions in regulating intestinal flora functions.

[0061] 1. Experimental Animals SPF grade Kunming mice, weight 18-20g.

[0062] 2. Samples to be tested Dietary fiber compositions prepared in Examples 1-5 and Comparative Examples 1-4.

[0063] 3. Animal grouping Kunming mice were randomly divided into a normal group, Example 1-5 groups and Comparative Example 1-4 groups according to body weight, with 8 mice in each group. Example 1-5 groups and Comparative Example 1-4 groups were intragastrically administered with the dietary fiber composition prepared in the corresponding Example or Comparative Example at a dosage of 50 mg / kg, and the normal group was intragastrically administered with an equal volume of physiological saline, once a day, for 14 consecutive days.

[0064] 4. Experimental methods Before administration, 1.0 g of feces from each group of mice was collected aseptically and inoculated on each culture medium at appropriate dilutions. After culture, the colonies were counted by Gram staining microscopy, and the number of bacteria per gram of wet feces was calculated. The logarithm was taken for statistical processing. 12 hours after the last administration, the number of bacteria in the feces of each group of mice was tested again using the same method.

[0065] Table 5 Effects of dietary fiber composition on bacterial flora in mouse feces

[0066] The results show: Compared with before administration, the bifidobacteria in the feces of the mice in Example 1-5 groups increased significantly (P<0.05), while the Bacteroides decreased significantly (P<0.05), indicating that the dietary fiber composition prepared in Examples 1-5 of the present invention has the effect of regulating the function of intestinal flora.

[0067] It is worth noting that: compared with before administration, there was no significant change in the bifidobacteria and Bacteroides in the feces of the mice in the comparative example 2 group (P>0.05), indicating that the dietary fiber composition prepared in the comparative example 2 of the present invention does not have the effect of regulating the function of intestinal flora.

[0068] Test Example 3: Evaluation of the laxative effect of dietary fiber composition 1. Experimental Animals SPF grade ICR mice, weighing 18-20g.

[0069] 2. Samples to be tested Dietary fiber compositions prepared in Examples 1-5 and Comparative Examples 1-4.

[0070] 3. Experimental methods After one week of adaptive feeding, the ICR mice were randomly divided into a normal group, a model group, Example 1-5 groups and Comparative Example 1-4 groups according to their body weight, with 8 mice in each group.

[0071] The Example 1-5 groups and the Comparative Example 1-4 groups were gavaged with the dietary fiber composition of the corresponding Examples and Comparative Examples, respectively, at a gavage dose of 50 mg / kg. The normal group and the model group were gavaged with an equal volume of physiological saline, once a day, for 14 consecutive days.

[0072] During the experiment, mice were free to drink water and eat. On the 7th and 14th days, mice were gavaged with 5 mg / kg loperamide hydrochloride to induce constipation model mice, and the normal group was gavaged with an equal volume of normal saline.

[0073] After the last oral gavage, rats were fasted but not watered for 24 h, and treated with ink by oral gavage at 1 mL / rat. After 0.5 h, the abdomen was opened and the small intestine (intestinal tissue from the pylorus at the upper end and the ileocecal part at the lower end) was obtained. The total length (cm) of the small intestine of the rats and the length of ink propagation (cm) were measured, and the ink propagation rate of the small intestine of the rats was calculated according to the following formula.

[0074] Small intestine ink propulsion rate = ink propulsion length / total small intestine length × 100%.

[0075] The results are shown in Table 6.

[0076] Table 6 Effects of dietary fiber composition on bowel movement

[0077] Note: Different letters in the table indicate significant differences among the groups, P < 0.05.

[0078] The results show: Compared with the normal group, the intestinal propulsion rate of mice in the model group was significantly reduced (P<0.05), indicating that the mouse model was successfully established.

[0079] Except for the comparative example 2 group, the intestinal propulsion rate of mice in each group was significantly increased compared with the model group (P<0.05), indicating that the dietary fiber compositions prepared in Examples 1-5 and comparative examples 1, 3 and 4 can promote intestinal peristalsis and have the effect of moisturizing the intestine and relieving constipation, while the dietary fiber composition prepared in comparative example 2 does not have the effect of moisturizing the intestine and relieving constipation.

[0080] The above is a further description of the present invention in conjunction with specific embodiments, but these embodiments are merely exemplary and do not constitute any limitation on the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solution of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the scope of protection of the present invention.

Claims

1. A dietary fiber composition for enhancing immunity and intestinal health, characterized in that: The invention comprises asparagus powder, liquorice powder, loquat powder, avocado powder and sea buckthorn powder, wherein the weight ratio of the loquat powder, avocado powder and sea buckthorn powder is 1-10:1-10:0.5-3.

2. The dietary fiber composition according to claim 1, characterized in that The raw materials are as follows by weight: 5-15 parts of asparagus powder, 3-20 parts of licorice powder, 1-10 parts of loquat powder, 1-10 parts of avocado powder and 0.5-3 parts of sea buckthorn powder.

3. The dietary fiber composition according to claim 2, characterized in that The raw materials are as follows by weight: 5-12 parts of asparagus powder, 5-15 parts of licorice powder, 1-5 parts of loquat powder, 3-8 parts of avocado powder and 1-2 parts of sea buckthorn powder.

4. The dietary fiber composition according to claim 1, characterized in that The raw materials are as follows by weight: 10 parts of asparagus powder, 8 parts of licorice powder, 3 parts of loquat powder, 5 parts of avocado powder and 1 part of sea buckthorn powder.

5. The dietary fiber composition according to claim 1, characterized in that The weight ratio of the loquat powder, avocado powder and sea buckthorn powder is 1-5:3-8:1-2.

6. The dietary fiber composition according to claim 5, characterized in that The weight ratio of the loquat powder, avocado powder and sea buckthorn powder is 3:5:

1.

7. The dietary fiber composition according to any one of claims 1 to 6, characterized in that The method for preparing the dietary fiber composition comprises the following steps: mixing asparagus powder, licorice powder, loquat powder, avocado powder and sea buckthorn powder in a prescribed amount and sieving.

8. Use of the dietary fiber composition according to any one of claims 1 to 7 in the preparation of functional foods, wherein the function is at least one of enhancing immunity, regulating intestinal flora function and moistening the intestine and promoting bowel movements.

9. The use according to claim 8, characterized in that: The functional food includes acceptable auxiliary materials in functional foods.

10. The use according to claim 8, characterized in that: The functional food is in the form of granules, powder, blocks or paste.

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