Sophora flower bud extract as well as preparation method and application thereof

Abstract

The invention discloses a pagodatree flower bud extract and a preparation method and application thereof, and the preparation method of the pagodatree flower bud extract comprises the following steps: grinding pagodatree flower buds to obtain pagodatree flower bud powder; the preparation method comprises the following steps: adding sophora flower bud powder into water, sterilizing, inoculating lactobacillus bulgaricus for fermentation treatment, and then adding compound enzyme for enzymolysis and enzyme deactivation treatment to obtain a mixed solution; and boiling and extracting the mixed solution, cooling, adding absolute ethyl alcohol, stirring, centrifuging, and removing the solvent to obtain the sophora flower bud extract. According to the preparation method, the sophora flower buds are ground, extraction of all effective components can be better promoted, then fermentation treatment is carried out through specific strains, protein in the sophora flower buds is decomposed through compound enzymes, protein polypeptide can be obtained, brass substances in the sophora flower buds can be effectively extracted through high-temperature extraction, and through the combined action of all the components, the content of the effective components in the sophora flower buds is increased. The sophora flower bud extract has more excellent oxidation resistance and can better meet market requirements.

Description

Technical Field

[0001] The invention relates to the technical field of plant extraction, and in particular to a sophora japonica flower extract and a preparation method and application thereof. Background Art

[0002] Sophora japonica is a deciduous tree of the genus Sophora in the Leguminosae family. Its bark is gray or dark gray, rough and longitudinally cracked; its inner bark is bright yellow and smelly; its branches are brown, green when young, with hairs, and obvious lenticels; its odd-pinnate compound leaves are alternate, ovate-oblong or ovate-lanceolate, its panicles are terminal, its flowers are milky white, its calyx is bell-shaped, its ovary is tubular, with fine hairs, and its style is curved; its pods are noded and bead-like, hairless, green, fleshy, and do not crack. Sophora japonica flowers bloom from July to August, and their fruiting period is from October to November. Sophora japonica is bitter and slightly cold in nature. Sophora japonica flowers have a blood pressure-lowering effect, can improve capillary function, and prevent bleeding caused by excessive capillary fragility and high permeability. It can prevent bleeding in patients with hypertension and diabetes.

[0003] The main ingredient of Sophora japonica rice is rutin (rutin) of quercetin, which belongs to the flavonoids and is a powerful antioxidant that can prevent the oxidation of low-density lipoprotein, thereby treating coronary heart disease. It also has the effects of lowering blood pressure, protecting myocardial ischemia, lowering blood lipids, anti-coagulation, and antibacterial and anti-inflammatory effects. In addition, quercetin also has central analgesic effects, enhances memory and has anti-depressant effects.

[0004] Sophora japonica seed is widely used because it contains rich rutin, quercetin and other substances. At present, the traditional extraction methods of Sophora japonica seed are mostly water extraction, alcohol extraction, alkali extraction and acid precipitation. This method only extracts the brass substance in Sophora japonica seed, ignoring other effective ingredients in Sophora japonica seed.

[0005] In view of this, the present invention is proposed. Summary of the invention

[0006] The object of the present invention is to provide a sophora japonica extract and a preparation method and application thereof. The sophora japonica extract prepared by the present invention has more excellent antioxidant properties and can better meet market demand.

[0007] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0008] The first aspect of the present invention provides a method for preparing a Sophora japonica extract, the method for preparing the Sophora japonica extract comprising the following steps:

[0009] (a) grinding the buds of Sophora japonica to obtain buds of Sophora japonica powder;

[0010] (b) adding the sophora japonica flour into the water, sterilizing and then inoculating Lactobacillus bulgaricus to carry out fermentation treatment, then, adding complex enzyme to carry out enzymolysis, enzyme inactivation treatment, and obtaining mixed solution;

[0011] (c) boiling the mixed solution for extraction, and after cooling, adding anhydrous ethanol to an ethanol concentration of 30% to 40%, stirring, centrifuging, and removing the solvent to obtain the sophora japonica flower extract.

[0012] Preferably, the particle size of the sophora japonica flour is not less than 10 meshes; and the mass ratio of the sophora japonica flour to water is 1:(15-30).

[0013] Preferably, the inoculation concentration of Lactobacillus bulgaricus is (1-5)×10 6 / ml;

[0014] The temperature of the fermentation treatment is 32-40° C. and the time is 40-60 hours.

[0015] Preferably, the added amount of the complex enzyme is 2% to 4% of the mass of the sophora japonica rice flour; the complex enzyme consists of papain and bromelain.

[0016] Preferably, the mass ratio of papain to bromelain is 1:(5-8).

[0017] Preferably, the enzymatic activity of the papain is not less than 100,000 U / g; the enzymatic activity of the bromelain is not less than 500,000 U / g.

[0018] Preferably, the enzymatic hydrolysis temperature is 50-60°C and the time is 1.5-2h.

[0019] Preferably, the boiling extraction time is 1 to 2 hours.

[0020] The second aspect of the present invention provides a sophora japonica flower extract prepared by the above preparation method.

[0021] The third aspect of the present invention provides a use of the Sophora japonica flower extract prepared by the above preparation method in the preparation of cosmetics.

[0022] Compared with the prior art, the beneficial effects of the present invention include at least:

[0023] The preparation method of the present invention can better promote the extraction of various effective components by grinding the sophora japonica buds, then use specific bacteria for fermentation treatment, and then decompose the protein in the sophora japonica buds by composite enzyme to obtain protein polypeptides, and then, through high-temperature extraction, quercetin and other brass substances in the sophora japonica buds can be effectively extracted, and through the joint action of various components, the sophora japonica bud extract has more excellent antioxidant properties and can better meet market demand.

[0024] The preparation method of the invention controls the preparation process and parameters, so that the sophora japonica extract has a higher yield and fully utilizes the effective components in the sophora japonica. DETAILED DESCRIPTION

[0025] The following embodiments of the technical solution of the present invention are described in detail in conjunction with the embodiments. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and are therefore only used as examples, and cannot be used to limit the protection scope of the present invention.

[0026] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0027] This embodiment provides a method for preparing a Sophora japonica extract, the method for preparing the Sophora japonica extract comprising the following steps:

[0028] (a) grinding the buds of Sophora japonica to obtain buds of Sophora japonica powder;

[0029] (b) adding the sophora japonica flour into the water, sterilizing and then inoculating Lactobacillus bulgaricus to carry out fermentation treatment, then, adding complex enzyme to carry out enzymolysis, enzyme inactivation treatment, and obtaining mixed solution;

[0030] (c) boiling the mixed solution for extraction, and after cooling, adding anhydrous ethanol to an ethanol concentration of 30% to 40%, stirring, centrifuging, and removing the solvent to obtain the sophora japonica flower extract.

[0031] The preparation method of the present invention can better promote the extraction of various effective components by grinding the sophora japonica buds, then use specific bacteria for fermentation treatment, and then decompose the protein in the sophora japonica buds by composite enzyme to obtain protein polypeptides, and then, through high-temperature extraction, quercetin and other brass substances in the sophora japonica buds can be effectively extracted, and through the joint action of various components, the sophora japonica bud extract has more excellent antioxidant properties and can better meet market demand.

[0032] In one embodiment, the inoculation concentration of Lactobacillus bulgaricus is (1-5)×10 6 / ml; the fermentation temperature is 32-40°C and the time is 40-60h.

[0033] In one embodiment, the particle size of the sophora japonica flour is not less than 10 mesh, specifically 10 mesh, 20 mesh or 50 mesh; the mass ratio of the sophora japonica flour to water is 1:(15-30).

[0034] In one embodiment, the added amount of the complex enzyme can be 2% to 4% of the mass of the sophora japonica rice flour, specifically 2%, 3% or 4%; the complex enzyme is composed of papain and bromelain.

[0035] In one embodiment, the mass ratio of papain to bromelain can be any value in the range of 1:(5-8), specifically 1:5, 1:6 or 1:8.

[0036] In one embodiment, the enzymatic activity of the papain is not less than 100,000 U / g; the enzymatic activity of the bromelain is not less than 500,000 U / g.

[0037] In one embodiment, the enzymatic hydrolysis temperature may be 50-60° C., and the time may be 1.5-2 h.

[0038] In one embodiment, the boiling extraction time may be 1 to 2 hours.

[0039] Another embodiment of the present invention provides a Sophora japonica flower extract prepared by the above preparation method.

[0040] Another embodiment of the present invention provides an application of the Sophora japonica extract prepared by the above preparation method in the preparation of cosmetics. Specifically, the Sophora japonica extract can be directly added to cosmetics, such as a body lotion or a facial mask.

[0041] The technical solution of the present invention is further described in detail below through specific embodiments.

[0042] Lactobacillus bulgaricus: purchased from Jiangsu Caiwei Biotechnology Co., Ltd.

[0043] Example 1

[0044] This embodiment is a method for preparing a Sophora japonica extract, and the method for preparing the Sophora japonica extract comprises the following steps:

[0045] (a) drying and grinding the buds of Sophora japonica, passing through a 10 mesh sieve to obtain bud powder of Sophora japonica;

[0046] (b) adding the sophora japonica flour to water in a mass ratio of 1:15, sterilizing, and then inoculating Lactobacillus bulgaricus for fermentation, then adding a composite enzyme for enzymolysis at 50°C for 2h, and then inactivating the enzyme to obtain a mixed solution, wherein the inoculation concentration of Lactobacillus bulgaricus is 1×10 6 / ml; the fermentation temperature is 40℃, the time is 60h; the amount of compound enzyme added is 2% of the mass of Sophora japonica flour; the compound enzyme is composed of papain and bromelain in a mass ratio of 1:5, the enzyme activity of papain is 100,000 U / g; the enzyme activity of bromelain is 500,000 U / g;

[0047] (c) boiling the mixture for 2 hours, and after cooling, adding anhydrous ethanol to a concentration of 30% and stirring, centrifuging, and removing the solvent to obtain the Sophora japonica flower extract.

[0048] Example 2

[0049] This embodiment is a method for preparing a Sophora japonica extract, and the method for preparing the Sophora japonica extract comprises the following steps:

[0050] (a) drying and grinding the buds of Sophora japonica, passing through a 10 mesh sieve to obtain bud powder of Sophora japonica;

[0051] (b) adding the sophora japonica flour to water in a mass ratio of 1:30, sterilizing, and then inoculating Lactobacillus bulgaricus for fermentation, then adding a composite enzyme for enzymolysis at 60° C. for 1.5 h, and then inactivating the enzyme to obtain a mixed solution, wherein the inoculation concentration of Lactobacillus bulgaricus is 5×10 6 / ml; the fermentation temperature is 32℃, the time is 40h; the amount of compound enzyme added is 4% of the mass of Sophora japonica flour; the compound enzyme is composed of papain and bromelain in a mass ratio of 1:8, the enzyme activity of papain is 100,000 U / g; the enzyme activity of bromelain is 500,000 U / g;

[0052] (c) boiling the mixed solution for extraction for 1 hour, and after cooling, adding anhydrous ethanol to a concentration of 40% and stirring, centrifuging, and removing the solvent to obtain the sophora japonica flower extract.

[0053] Example 3

[0054] This embodiment is a method for preparing a Sophora japonica extract, and the method for preparing the Sophora japonica extract comprises the following steps:

[0055] (a) drying and grinding the buds of Sophora japonica, passing through a 10 mesh sieve to obtain bud powder of Sophora japonica;

[0056] (b) adding the sophora japonica flour to water in a mass ratio of 1:25, sterilizing, and then inoculating Lactobacillus bulgaricus for fermentation, then adding a composite enzyme for enzymolysis at 55°C for 2h, and then inactivating the enzyme to obtain a mixed solution, wherein the inoculation concentration of Lactobacillus bulgaricus is 2×10 6 / ml; the fermentation temperature is 35℃, the time is 50h; the amount of compound enzyme added is 3% of the mass of Sophora japonica flour; the compound enzyme is composed of papain and bromelain in a mass ratio of 1:6, the enzyme activity of papain is 100,000 U / g; the enzyme activity of bromelain is 500,000 U / g;

[0057] (c) boiling the mixed solution for 1.5 hours, cooling it, adding anhydrous ethanol to a concentration of 35% and stirring, centrifuging, and removing the solvent to obtain the Sophora japonica flower extract.

[0058] Comparative Example 1

[0059] This comparative example is a method for preparing a Sophora japonica extract, and the method for preparing the Sophora japonica extract comprises the following steps:

[0060] (a) drying and grinding the buds of Sophora japonica, passing through a 10 mesh sieve to obtain bud powder of Sophora japonica;

[0061] (b) adding the sophora japonica flour to water in a mass ratio of 1:25, adding a composite enzyme for enzymolysis at 55°C for 2h, and inactivating the enzyme to obtain a mixed solution, wherein the amount of the composite enzyme added is 3% of the mass of the sophora japonica flour; the composite enzyme is composed of papain and bromelain in a mass ratio of 1:6, the enzymatic activity of the papain is 100,000 U / g; and the enzymatic activity of the bromelain is 500,000 U / g;

[0062] (c) boiling the mixed solution for 1.5 hours, cooling it, adding anhydrous ethanol to a concentration of 35% and stirring, centrifuging, and removing the solvent to obtain the Sophora japonica flower extract.

[0063] Comparative Example 2

[0064] This comparative example is a method for preparing a Sophora japonica extract, and the method for preparing the Sophora japonica extract comprises the following steps:

[0065] (a) drying and grinding the buds of Sophora japonica, passing through a 10 mesh sieve to obtain bud powder of Sophora japonica;

[0066] (b) adding the sophora japonica flour to water in a mass ratio of 1:25, sterilizing, and then inoculating Lactobacillus bulgaricus for fermentation, then adding a composite enzyme for enzymolysis at 55°C for 2h, and then inactivating the enzyme to obtain a mixed solution, wherein the inoculation concentration of Lactobacillus bulgaricus is 2×10 6 / ml; the fermentation temperature is 35℃, the time is 50h, and the added amount of complex enzyme is 3% of the mass of Sophora japonica flour; the complex enzyme is composed of papain and bromelain in a mass ratio of 1:1, the enzyme activity of papain is 100,000 U / g; the enzyme activity of bromelain is 500,000 U / g;

[0067] (c) boiling the mixed solution for 1.5 hours, cooling it, adding anhydrous ethanol to a concentration of 35% and stirring, centrifuging, and removing the solvent to obtain the Sophora japonica flower extract.

[0068] Comparative Example 3

[0069] This comparative example is a method for preparing a Sophora japonica extract, and the method for preparing the Sophora japonica extract comprises the following steps:

[0070] (a) drying and grinding the buds of Sophora japonica, passing through a 10 mesh sieve to obtain bud powder of Sophora japonica;

[0071] (b) adding the sophora japonica flour to water in a mass ratio of 1:25, sterilizing, and then inoculating Lactobacillus bulgaricus for fermentation, then adding a composite enzyme for enzymolysis at 55°C for 2h, and then inactivating the enzyme to obtain a mixed solution, wherein the inoculation concentration of Lactobacillus bulgaricus is 2×10 6 / ml; the fermentation temperature is 35℃, the time is 50h, and the added amount of complex enzyme is 3% of the mass of Sophora japonica flour; the complex enzyme is composed of neutral protease and bromelain in a mass ratio of 1:6, the enzyme activity of neutral protease is 100,000 U / g; the enzyme activity of bromelain is 500,000 U / g;

[0072] (c) boiling the mixed solution for 1.5 hours, cooling it, adding anhydrous ethanol to a concentration of 35% and stirring, centrifuging, and removing the solvent to obtain the Sophora japonica flower extract.

[0073] Comparative Example 4

[0074] This comparative example is a method for preparing a Sophora japonica extract, and the method for preparing the Sophora japonica extract comprises the following steps:

[0075] (a) drying and grinding the buds of Sophora japonica, passing through a 10 mesh sieve to obtain bud powder of Sophora japonica;

[0076] (b) adding the sophora japonica flour to water in a mass ratio of 1:25 and treating at 55° C. for 2 h to obtain a mixed solution;

[0077] (c) boiling the mixed solution for 1.5 hours, cooling it, adding anhydrous ethanol to a concentration of 35% and stirring, centrifuging, and removing the solvent to obtain the Sophora japonica flower extract.

[0078] Experimental example

[0079] The Sophora japonica extract was prepared according to the preparation methods of Example 3 and Comparative Examples 1 to 4, and the yield was calculated according to Sophora japonica extract / Sophora japonica × 100%. The calculation results are shown in Table 1.

[0080] The hydroxyl free radical scavenging rate of different Sophora japonica extracts was tested.

[0081] The above different Sophora japonica flower extracts were prepared into a 10 mg / ml mixed solution;

[0082] Take two 25ml colorimetric tubes (sample tube and blank tube), add 5ml1mmol / L ferrous sulfate solution and 5ml3mmol / L H202 solution respectively, add 1ml sample to the sample tube and 1ml distilled water to the blank tube, mix well and make up to the scale with 3mmol / L salicylic acid solution. After reacting in constant temperature water at 37℃ for 15min, use a spectrophotometer to measure the absorbance of each tube at a wavelength of 510nm, and adjust to zero with 3mmol / L salicylic acid solution.

[0083] The clearance rate was calculated according to the following formula: clearance rate = (blank tube absorbance - sample tube absorbance) / blank tube absorbance × 100%. The calculation results are shown in Table 1.

[0084] Table 1

[0085] Group Yield Clearance Example 3 28.7％ 89.1％ Comparative Example 1 24.2％ 78.5％ Comparative Example 2 22.3％ 60.6％ Comparative Example 3 25.1％ 63.2％ Comparative Example 4 19.7％ 51.8％

[0086] From Table 1, we can see that:

[0087] The Sophora japonica flower extract prepared in the embodiment of the present application can better remove free radicals and has a better antioxidant effect.

[0088] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and specification of the present invention.

Claims

1. A method for preparing a Sophora japonica extract, characterized in that: The steps include: (a) grinding the buds of Sophora japonica to obtain buds of Sophora japonica powder; (b) adding the sophora japonica flour into the water, sterilizing and then inoculating Lactobacillus bulgaricus to carry out fermentation treatment, then, adding complex enzyme to carry out enzymolysis, enzyme inactivation treatment, and obtaining mixed solution; (c) boiling the mixed solution for extraction, and after cooling, adding anhydrous ethanol to an ethanol concentration of 30% to 40%, stirring, centrifuging, and removing the solvent to obtain the sophora japonica flower extract.

2. The method for preparing the Sophora japonica extract according to claim 1, characterized in that: The particle size of the sophora japonica flour is not less than 10 meshes; the mass ratio of the sophora japonica flour to water is 1:(15-30).

3. The method for preparing the Sophora japonica extract according to claim 1, characterized in that: The inoculation concentration of the Lactobacillus bulgaricus is (1-5)×10 6 / ml; The temperature of the fermentation treatment is 32-40° C. and the time is 40-60 hours.

4. The method for preparing the Sophora japonica extract according to claim 1, characterized in that: The added amount of the complex enzyme is 2% to 4% of the mass of the locust bean flour; the complex enzyme consists of papain and bromelain.

5. The method for preparing the Sophora japonica extract according to claim 3, characterized in that: The mass ratio of papain to bromelain is 1:(5-8).

6. The method for preparing the Sophora japonica extract according to claim 3, characterized in that: The enzymatic activity of the papain is not less than 100,000 U / g; the enzymatic activity of the bromelain is not less than 500,000 U / g.

7. The method for preparing the Sophora japonica extract according to claim 1, characterized in that: The enzymolysis temperature is 50-60° C. and the time is 1.5-2 hours.

8. The method for preparing the Sophora japonica extract according to claim 1, characterized in that: The boiling extraction time is 1 to 2 hours.

9. The Sophora japonica flower extract obtained by the preparation method according to any one of claims 1 to 8.

10. Use of the Sophora japonica flower extract obtained by the preparation method according to any one of claims 1 to 8 in the preparation of cosmetics.