Composition with effect of treating osteoporosis as well as preparation method and application of composition

Abstract

The invention discloses a composition with an effect of treating osteoporosis and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 1-10 parts of collagen peptide extract, 1-10 parts of ground beetle, scorpion and centipede extract and 1-10 parts of pseudo-ginseng. Under the guidance of traditional Chinese medicine theories, all the components are screened through a large number of experiments and are scientifically matched to obtain the traditional Chinese medicine composition. Experimental results show that the composition provided by the invention can effectively treat osteoporosis, tonify kidney and strengthen bone, slow down bone loss, improve bone mineralization, promote calcium absorption, maintain calcium metabolism balance and the like, is safe and effective, and can be developed into medicines for preventing and treating osteoporosis.

Description

Technical Field

[0001] The invention relates to a composition, in particular to a composition capable of treating osteoporosis, a preparation method thereof and an application thereof. Background Art

[0002] Osteoporosis (OP) is a systemic metabolic bone disease characterized by decreased bone density, destruction of bone tissue microstructure, increased bone brittleness, and easy fracture. It is one of the diseases with the highest morbidity, disability and mortality rates in the world, and it also costs a lot of money in medical treatment. Osteoporosis is called a "silent disease" because it does not show obvious symptoms in the early stages until a fracture occurs. Patients' perception of fracture risk is often underestimated, and it is only discovered when X-rays or bone mineral density (BMD) measurements are taken. Most patients often seek medical treatment for fractures after minor activities, trauma, weight bearing, bending over, or falling. If patients do not receive timely diagnosis and treatment, a series of complications will eventually occur, greatly affecting their life, health, and daily life. Therefore, the further development of anti-osteoporosis drugs is of extremely important clinical significance for its prevention and treatment.

[0003] Chinese and Western medicine each have their own advantages in the diagnosis and treatment of OP. There is no concept of "osteoporosis" in traditional Chinese medicine, but it belongs to the category of "bone atrophy", "bone dryness", "bone arthritis", etc. The lesions are in the bones, and the root is in the kidney. The cause is mainly kidney deficiency, which is closely related to the liver, spleen, and blood stasis. The syndrome is deficiency of the root and excess of the symptoms. Modern Chinese medicine syndrome differentiation divides osteoporosis into four types: kidney yang deficiency, spleen and kidney yang deficiency, liver and kidney yin deficiency, and blood stasis and qi stagnation. Kidney Yang Deficiency Type: Yang deficiency of the body or kidney deficiency due to old age, dysfunction of warming, and lack of source of bone marrow biochemistry. It is advisable to tonify the kidney and strengthen yang, strengthen the tendons and bones; Spleen and Kidney Yang Deficiency Type: Kidney is the "innate foundation" and spleen is the "acquired foundation". Spleen deficiency and unhealthy, lack of source of qi and blood biochemistry will lead to deficiency of essence and empty marrow, malnutrition of bone marrow, and atrophy of limbs. It is advisable to tonify the spleen and kidney, and strengthen the tendons and bones; Liver and Kidney Yin Deficiency Type: The liver stores blood and the kidney stores essence. Insufficient liver blood will lead to lack of nourishment of essence and liver and kidney essence. It is advisable to nourish the liver and kidney, and replenish essence and strengthen bones; Blood stasis and Qi stagnation Type: Old age or trauma and contusion lead to poor circulation of qi and blood, and loss of blood nourishment to bones. It is advisable to regulate qi and activate blood circulation, and resolve blood stasis and relieve pain.

[0004] Currently, unilateral drugs for osteoporosis have some defects in the treatment of osteoporosis patients. Therefore, it is of profound clinical significance to actively develop compound drugs that can complement each other's advantages, improve the anti-osteoporosis efficacy, and reduce adverse reactions. Summary of the invention

[0005] The first object of the present invention is to provide a composition for promoting the recovery of osteoporosis, which has a scientific and reasonable ratio, definite efficacy, safe clinical use and low adverse reactions.

[0006] The ability of bones to resist fractures and mechanical forces depends not only on the amount of bone minerals, but also on the collagen framework. Bone collagen can bind and store growth factors and cytokines, such as insulin-like growth factors (IGF) I and II, which are released during bone degradation and promote bone formation. The present invention uses waste aquatic resources such as fish scales, fish bones or fish skin as collagen extraction sources, prepares collagen extracts by screening and optimizing preparation conditions, and is compatible with traditional Chinese medicines such as earthworms, scorpions, centipedes and Panax notoginseng, which has a good effect of preventing and treating osteoporosis.

[0007] Another object of the present invention is to provide a preparation method and application of the composition.

[0008] In order to achieve the above purpose, the technical solution adopted by the present invention is:

[0009] A method for preparing a composition for treating osteoporosis, comprising collagen peptide extract, earthworm, scorpion, centipede extract and notoginseng powder.

[0010] The method for preparing proprotein provided by the present invention comprises the following steps:

[0011] Fish scales, fish bones and fish skin are used as raw materials, powdered and then washed, soaked in 95% ethanol for 0.5-2 hours, soaked in NaOH solution for 1-3 hours, soaked in 0.2%-0.5% H2SO4 (W / V) solution for 1-3 hours, washed, reacted at pH 4-6 and temperature 60-70°C for 1-3 hours, centrifuged, took the supernatant, and freeze-dried to obtain collagen extract.

[0012] As a preferred embodiment, the preparation method of extracts of earthworm, scorpion and centipede comprises the following steps: taking earthworm, scorpion and centipede respectively, adding 5 to 10 times the volume of water of the medicinal materials respectively, extracting 1 to 3 times, each time for 1 to 5 hours, combining the filtrate, concentrating to obtain water extract, freeze-drying to obtain earthworm, scorpion and centipede extracts respectively.

[0013] As a preferred embodiment, the above-mentioned composition for treating osteoporosis can be extracted by methods including decoction, reflux, ultrasound, percolation or immersion.

[0014] As a preferred embodiment, the preparation method of Panax notoginseng powder includes the following steps: cleaning the dirt on the surface of Panax notoginseng, discarding Panax notoginseng with poor quality, removing the epidermis of Panax notoginseng, rinsing the adhered epidermal debris, slicing, and putting the sliced ​​Panax notoginseng into a grinding equipment for preliminary grinding, and after grinding, putting the preliminary crushed Panax notoginseng into the grinding equipment again for fine grinding to obtain Panax notoginseng powder.

[0015] The invention provides a product for promoting bone density increase and resisting osteoporosis, comprising the following five components: collagen peptide extract, earthworm extract, scorpion extract, centipede extract and Panax notoginseng powder, wherein the mass ratio of each component is 1-10:1-10:1-10:1-10:1-10:1-10.

[0016] Preferably, in any of the above schemes, the mass ratio of the collagen peptide extract, the earthworm extract, the scorpion extract, the centipede extract and the Panax notoginseng powder is 1-10: 1-5: 1-5: 1-5: 5-10.

[0017] Preferably, in any of the above schemes, the mass ratio of the collagen peptide extract, the earthworm extract, the scorpion extract, the centipede extract and the Panax notoginseng powder is 5-10:5-10:5-10:5-10:5-10:1-5.

[0018] The composition for treating osteoporosis of the present invention comprises various raw material extracts and pharmaceutically acceptable carriers which can be adjuvants for oral preparations or adjuvants for parenteral administration.

[0019] The administration route may be oral administration, topical administration, etc., wherein oral preparations include capsules, soft capsules, granules, oral liquids, tablets, dripping pills, etc. Suitable excipients include, but are not limited to, excipients, lubricants, binders, disintegrants, water-soluble polymers, inorganic salts, solvents, dissolution aids, suspending agents, isotonic agents, buffers, preservatives, antioxidants, colorants, sweeteners, acidulants, foaming agents and flavoring agents, etc., including but not limited to conventional excipients such as starch, sucrose, lactose, powdered sugar, glucose, mannitol, xylitol, polyethylene glycol, isopropanol, Tween-80, glycerol, propylene glycol, sodium microcrystalline cellulose, dextrin, cyclodextrin, sodium chloride, vitamin C, cysteine, citric acid, sodium thiosulfate, sodium sulfite, stearate and gelatin. The later preparation process and equipment of the preparation belong to conventional techniques in the pharmaceutical field, which are not limited by the present invention and are not described in detail herein.

[0020] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0021] The present invention is based on the theory of traditional Chinese medicine and modern medical knowledge, adopts the theory of syndrome differentiation and treatment to screen the formula, and makes a scientific ratio. The components produce a synergistic effect, activate osteoblasts, promote the synthesis of organic and inorganic matter in trabeculae, rebuild the trabecular structure, and restore the bones from the osteoporotic state to the dense bone state, restore the hard and soft characteristics of the bones, solve the pain of bone pain caused by osteoporosis, and eliminate the risk of fractures in osteoporotic patients. DETAILED DESCRIPTION

[0022] The present invention is further explained below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, various equivalent modifications of the present invention by those skilled in the art all fall within the scope defined by the claims attached to this application.

[0023] Example 1

[0024] A method for preparing a composition having an osteoporosis treating effect comprises the following steps:

[0025] (1) Take 1 kg of dry fish scales, crush them into floccules and wash them, add 6 L of 95% ethanol by volume to soak for 1 hour, wash them, and then soak them in 6 L of NaOH solution for 1 hour. After washing, soak the sample in 0.2%-0.5% (W / V) H2SO4 solution for 3 hours. After washing, add 8 L of water, adjust the pH value to 5, react at 60°C for 3 hours, centrifuge, take the supernatant, and lyophilize it to obtain collagen extract.

[0026] (2) Take 500 g of Eupolyphaga sinensis, add 6 times the volume of water by weight of the medicinal material, decoct and extract twice, each time for 1 hour, combine the filtrate, concentrate, and freeze-dry to obtain Eupolyphaga sinensis extract; take scorpion and centipede according to the same operation, extract and freeze-dry to obtain scorpion and centipede extracts.

[0027] (3) Take the washed Panax notoginseng, remove the epidermis of the Panax notoginseng, rinse the attached epidermis debris, slice the Panax notoginseng, and put the sliced ​​Panax notoginseng into a grinding device for preliminary grinding. After the grinding, put the preliminary crushed Panax notoginseng into the grinding device again for fine grinding to obtain Panax notoginseng powder.

[0028] (4) The above collagen extract, earthworm, scorpion, centipede extract and Panax notoginseng powder are mixed in a weight ratio of 10:5:5:5:10, and then mixed with the auxiliary material starch, and then prepared into capsules through sol, encapsulation, shaping, drying and polishing.

[0029] Example 2

[0030] A method for preparing a composition having an osteoporosis treating effect comprises the following steps:

[0031] (1) Take 500 g of dried fish scales, crush them into floccules and wash them, soak them in 3 L of 95% ethanol for 2 h, wash them, soak them in 3 L of NaOH solution for 2 h, and then soak the sample in 0.2%-0.5% (W / V) H2SO4 solution for 2 h, adjust the pH value to 5, react at 70°C for 2 h, centrifuge, take the supernatant, and obtain a collagen extract.

[0032] (2) Take 200 g of Eupolyphaga sinensis, add 8 times the volume of water of the medicinal material, decoct and extract twice, each time for 2 hours, combine the filtrates, concentrate, and freeze-dry to obtain Eupolyphaga sinensis extract; take scorpion and centipede respectively according to the same operation, extract and freeze-dry to obtain scorpion and centipede extracts.

[0033] (3) Take the washed Panax notoginseng, remove the epidermis of the Panax notoginseng, rinse the attached epidermis debris, slice the Panax notoginseng, and put the sliced ​​Panax notoginseng into a grinding device for preliminary grinding. After the grinding, put the preliminary crushed Panax notoginseng into the grinding device again for fine grinding to obtain Panax notoginseng powder.

[0034] (4) Mix the collagen, Chinese earthworm, scorpion, centipede extract and Panax notoginseng powder in a weight ratio of 10:10:10:10:5, add a diluent and mix evenly, granulate and dry to make granules.

[0035] Example 3

[0036] A method for preparing a composition having an osteoporosis treating effect comprises the following steps:

[0037] (1) Take 5 kg of dry fish scales, crush them into floccules and wash them, add 20 L of 95% ethanol to soak for 0.5 h, wash them, soak them in 20 L of NaOH solution for 1 h, and then soak the sample in 0.2%-0.5% (W / V) H2SO4 solution for 1.5 h. After washing, adjust the pH value to 6, react at 65°C for 1.5 h, centrifuge, take the supernatant, and lyophilize it to obtain collagen extract.

[0038] (2) Take 2.5 kg of Eupolyphaga sinensis, add 6 times the volume of water of the medicinal material, decoct and extract twice, each time for 1 hour, combine the filtrate, concentrate, and freeze-dry to obtain Eupolyphaga sinensis extract; take scorpion and centipede respectively according to the same operation, extract and freeze-dry to obtain scorpion and centipede extracts.

[0039] (3) Take the washed Panax notoginseng, remove the epidermis of the Panax notoginseng, rinse the attached epidermis debris, slice the Panax notoginseng, and put the sliced ​​Panax notoginseng into a grinding device for preliminary grinding. After the grinding, put the preliminary crushed Panax notoginseng into the grinding device again for fine grinding to obtain Panax notoginseng powder.

[0040] (4) Mix collagen, Chinese earthworm, scorpion, centipede extract and Panax notoginseng powder in a weight ratio of 8:8:8:8:1, add adhesive auxiliary materials and mix evenly, granulate, dry and make into pills.

[0041] Example 4

[0042] A method for preparing a composition having an osteoporosis treating effect comprises the following steps:

[0043] (1) Take 3.5 kg of dry fish scales, crush them into floccules and then wash them, add 3 L of 95% ethanol to soak for 1 hour, wash, soak in 3 L of NaOH solution for 3 hours, and then soak the sample in 0.2%-0.5% (W / V) H2SO4 solution for 3 hours. After washing, adjust the pH value to 4, react at 65°C for 2 hours, centrifuge, take the supernatant, and obtain collagen extract.

[0044] (2) Take 1 kg of Eupolyphaga sinensis, add 6 times the volume of water of the medicinal material, decoct and extract twice, each time for 1 hour, combine the filtrate, concentrate, and freeze-dry to obtain Eupolyphaga sinensis extract; take scorpion and centipede respectively according to the same operation, extract and freeze-dry to obtain scorpion and centipede extracts.

[0045] (3) Take the washed Panax notoginseng, remove the epidermis of the Panax notoginseng, rinse the attached epidermis debris, slice the Panax notoginseng, and put the sliced ​​Panax notoginseng into a grinding device for preliminary grinding. After the grinding, put the preliminary crushed Panax notoginseng into the grinding device again for fine grinding to obtain Panax notoginseng powder.

[0046] (4) After mixing collagen, Chinese earthworm, scorpion, centipede extract and Panax notoginseng powder in a weight ratio of 5:1:1:1:1, add adhesive auxiliary materials and mix evenly, granulate, dry and make into pills.

[0047] Example 5 Pharmacological Experimental Study

[0048] 1. Experimental Animals

[0049] SD rats, clean grade, female, 200-230 g.

[0050] 2. Experimental drugs

[0051] 2.1 Positive drug: Alendronate sodium (5 mg / kg / d.bw).

[0052] 2.2 Test drugs:

[0053] The collagen extract, the extracts of Eupolyphaga sinensis, Scorpion, Centipede and Panax notoginseng powder prepared according to the methods of Examples 1 and 2 of the present invention respectively were mixed; the dosages were divided into a low-dose group: 250 mg / kg / d of the mixed extract and a high-dose group: 750 mg / kg / d of the mixed extract.

[0054] 3. Experimental Methods

[0055] 3.1 Experimental modeling, grouping and drug administration

[0056] Female rats were raised for two weeks and randomly divided into 6 groups after their body weight increased to 230-260 g, namely, sham operation group, model group, positive drug group, low-dose group of the composition of Example 1 and Example 2, and high-dose group of the composition. All animals were raised under the same conditions, with free access to food and water, and the rats were subjected to ovariectomy.

[0057] 3% sodium pentobarbital solution (40 mg / kg, i.e. 0.2 ml / 100 g) was injected intraperitoneally for anesthesia. After anesthesia, the rats were fixed with the abdomen facing upwards, and the hair was cleaned in a 4 cm × 3 cm hair-cutting range under the xiphoid process. The surgical site was disinfected with 75% alcohol, covered with a hole towel, and a longitudinal incision was made along the linea alba at about 1.5 cm below the xiphoid process. The incision was about 1.7 cm long, and the abdominal muscles and peritoneum were separated to expose the abdominal cavity. Carefully search for the ovary along the lower horn of the uterus upwards, and then slowly bring the ovary out, and use catgut to ligate the fallopian tube and its surrounding adipose tissue and blood vessels. The model group, the positive drug group, and the high and low dose groups of the combination of Example 1 and Example 2 had their bilateral ovaries completely removed; the sham operation group had an equal amount of adipose tissue around the ovaries removed. Carefully stop bleeding and suture the surgical incision in layers. Apply erythromycin ointment to the external wound, and inject penicillin sodium intramuscularly, 200,000 units per day, for 5 consecutive days.

[0058] After the surgical wounds of each group recovered, intragastric administration began on the 7th day for 3 months. Intragastric administration: positive drug group: alendronate sodium 5 mg / kg / d, low-dose group of the combination of Example 1 and Example 2: 250 mg / kg / d: high-dose group: 750 mg / kg / d, sham operation group and model group were given the same amount of distilled water. Once a day, the dosage was adjusted once a week according to body weight.

[0059] 3.2 Analysis of rat bone tissue morphology

[0060] The left femur of the mouse was removed, the surrounding soft tissue was stripped off, and it was fixed with 4% paraformaldehyde for 24 to 48 hours, then decalcified with EDTA decalcification solution. After decalcification, the tissue was gradient dehydrated, and the specimen was placed according to the experimental requirements, embedded in paraffin, sliced ​​(slice thickness 5 μm), and then HE stained. The changes in trabecular microstructure were observed under a light microscope and photographed.

[0061] 3.3 Micro-CT analysis of rat femur

[0062] Micro-CT scanning was performed on the right femur samples of rats. The 0-100th cross-section of the proximal distal femoral growth plate was used as the region of interest for evaluating the trabecular structure, and the mid-femoral section of about 1 mm in length (100 cross-sections) was used as the region of interest for evaluating the cortical bone. Three-dimensional reconstruction was performed using analysis software. Bone morphological parameters such as cortical bone mineral density (BMD), bone volume / total volume (BV / TV), trabecular thickness (Tb.Th) and trabecular spacing (Tb.Sp) were analyzed.

[0063] 3.4 Determination of serum bone metabolism indexes in rats

[0064] The levels of serum osteocalcin (BGP), serum alkaline phosphatase (BALP) and type I procollagen N-terminal propeptide (PINP) in rats in each group were determined strictly according to the instructions of the ELISA kit.

[0065] 4. Experimental Results

[0066] The specific experimental results are shown in Table 1 and Table 2.

[0067] Table 1 Results of serum biochemical tests on ovariectomized rats in each group (unit: ng / ml, n=12)

[0068]

[0069] Note: Compared with the model group, * P<0.05, ** P<0.01; compared with the sham operation group, # P<0.05, ## P<0.01

[0070] According to the experimental results in Table 1, the differences in serum bone metabolism indicators between the model group and the sham operation group were statistically significant, indicating that the model was successfully established.

[0071] Increased serum osteocalcin levels can slow down the healing rate of bone tissue; the expression level of PINP reflects the formation of new bone; BALP is used to evaluate bone formation and bone turnover. The experimental results show that the collagen extracts of Examples 1 and 2, the extracts of Eupolyphaga sinensis, Scorpion, Centipede and Panax notoginseng powder can significantly increase the serum BGP level of ovariectomized osteoporotic rats and significantly reduce the levels of BALP and PINP, indicating that the compositions provided by Examples 1 and 2 of the present invention can reduce bone metabolism by affecting the activity of osteoblasts and osteoclasts, indicating that the combination of Examples 1 and 2 of the present invention has a good effect on resisting osteoporosis caused by ovariectomized rats, and the effect of Example 2 is better than that of Example 1.

[0072] Table 2 Effects on bone density and bone microstructure in OVX rats ( n=12)

[0073]

[0074] Note: Compared with the model group, * P<0.05, ** P<0.01; compared with the sham operation group, # P<0.05, ## P<0.01

[0075] As shown in Table 2, compared with the sham operation group, the model group had a significantly decreased bone density, and compared with the model control group, the positive drug group and the compositions of Examples 1 and 2 had a significantly increased bone density, which could significantly improve the bone density and bone microstructure of rats. Among them, the ratio of Example 2 was better than that of Example 1.

[0076] The present invention is based on traditional Chinese medicine theory and modern medical knowledge, adopts the theory of syndrome differentiation and treatment to screen Chinese medicine prescriptions, and the composition ratio is scientific and reasonable. The above experimental results show that the composition provided by the present invention can effectively prevent and treat osteoporosis, has the effects of increasing bone density and improving bone strength, and is expected to be developed into a new drug for preventing and treating osteoporosis.

[0077] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition for treating osteoporosis, characterized in that: It contains 1 to 10 parts of collagen peptide extract, 1 to 10 parts of extracts of earthworm, scorpion and centipede, and 1 to 10 parts of Panax notoginseng.

2. The composition for treating osteoporosis according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: the mass ratio of collagen peptide extract, earthworm extract, scorpion extract, centipede extract and notoginseng powder is 10:5:5:5:

10.

3. The composition for treating osteoporosis according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: the mass ratio of collagen peptide extract, earthworm extract, scorpion extract, centipede extract and notoginseng powder is 10:10:10:10:

5.

4. The composition for treating osteoporosis according to any one of claims 1 to 3, characterized in that: The collagen peptide extract is prepared by the following method: fish scales, fish bones and fish skin are used as raw materials, powdered and then washed, soaked in 95% ethanol for 0.5-2h, soaked in NaOH solution for 1-3h after washing, soaked in 0.2%-0.5% H2SO4 (W / V) solution for 1-3h after washing, washed, reacted at a pH value of 4-6 and a temperature of 60-70°C for 1-3h, centrifuged, took the supernatant, and freeze-dried to obtain the collagen extract.

5. The composition for treating osteoporosis according to any one of claims 1 to 3, characterized in that: The extracts of the earthworm, scorpion and centipede are prepared by the following method: taking the earthworm, scorpion and centipede respectively, adding 5 to 10 times the volume of water of the medicinal materials respectively, extracting 1 to 3 times, each time for 1 to 5 hours, combining the filtrate, concentrating to obtain a water extract, freeze-drying to obtain the earthworm, scorpion and centipede extracts.

6. The composition for treating osteoporosis according to claim 5, characterized in that: Extraction methods include decoction, reflux, sonication, percolation or maceration.

7. The composition for treating osteoporosis according to any one of claims 1 to 3, characterized in that: The raw material extracts and pharmaceutically acceptable carriers are prepared into medicines in the form of tablets, granules, capsules, pills and oral liquids.

8. The method for preparing the composition for treating osteoporosis according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) Using fish scales, fish bones and fish skin as raw materials, powdering and washing, soaking in 95% ethanol for 0.5-2h, soaking in NaOH solution for 1-3h, soaking in 0.2%-0.5% H2SO4 (W / V) solution for 1-3h, washing, reacting at pH 4-6 and temperature 60-70°C for 1-3h, centrifuging, taking the supernatant, lyophilizing, obtaining collagen extract for standby use; (2) taking the Chinese earthworm, scorpion and centipede, adding 5 to 10 times the volume of the medicinal material of water, extracting 1 to 3 times, each time for 1 to 5 hours, combining the filtrate, concentrating to obtain a water extract, freeze-drying to obtain the Chinese earthworm, scorpion and centipede extract, and setting aside; (3) Using Panax notoginseng as raw material, clean the surface dirt, discard the Panax notoginseng with poor quality, remove the skin of Panax notoginseng, rinse the attached skin debris, slice it, and put the sliced ​​Panax notoginseng into a crushing equipment for preliminary crushing. After crushing, put the preliminary crushed Panax notoginseng into the crushing equipment again for fine crushing and set aside. (4) The collagen extract prepared in step (1), the extracts of earthworm, scorpion and centipede prepared in step (2) and the Panax notoginseng powder prepared in step (3) are respectively taken according to the weight proportions as described in any one of claims 1 to 3, and a pharmaceutically acceptable carrier is added to prepare a drug in the form of tablets, granules, capsules, pills or oral liquid.

9. Use of the composition having the effect of treating osteoporosis according to any one of claims 1 to 3 in the preparation of a drug for preventing and treating osteoporosis.

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