Centrifugal sample mixing device, centrifugal sample mixing system and centrifugal sample mixing method

Abstract

A centrifugal sample mixing device, a centrifugal sample mixing system and a centrifugal sample mixing method.The centrifugal sample mixing device comprises a bearing disc, a heating and cooling module, a moving module and a centrifugal driving module, the bearing disc is used for bearing a centrifugal container, different fluids are contained in the centrifugal container, the moving module is used for driving the heating and cooling module to move, and the centrifugal driving module is used for driving the heating and cooling module to move. The heating and cooling module is attached to or separated from the centrifugal container; the centrifugal driving module is used for driving the bearing disc to rotate after the temperature increasing and decreasing module is separated from the bearing disc, so that different fluids in the centrifugal container are mixed; the temperature increasing and decreasing module is used for heating and / or cooling fluid in the centrifugal container after being attached to the centrifugal container. The centrifugal sample mixing device disclosed by the invention can realize synchronous rotation motion and heating and cooling reaction of the centrifugal container, and is simple and compact in structure, small in size, small in occupied space, simple to operate and high in centrifuging, heating and cooling efficiency; the problems of reagent pollution and waste do not exist, the experiment success rate is improved, and the cost is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of biochemical substance analysis, and in particular to a centrifugal sample mixing device, a centrifugal sample mixing system and a centrifugal sample mixing method. Background Art

[0002] Polymerase chain reaction (PCR) uses a segment of DNA as a template, and with the participation of DNA polymerase and nucleotide substrate, the segment of DNA is amplified to a sufficient amount for structural and functional analysis. In existing PCR detection technology, different reagents are usually required to be transferred and mixed, and then the mixed reagents are centrifuged, and then the centrifuged mixed reagents are transferred to a chip to start cyclic heating and cooling to achieve the PCR reaction. However, in the above PCR detection process, the mixing, centrifugation and heating and cooling of reagents all require the use of different equipment, which takes up a large space. In addition, the transfer of multiple steps of reagents is involved in the middle, which is inefficient and easily causes reagent contamination, reducing the success rate of PCR detection. Summary of the invention

[0003] In order to solve at least one of the above defects, it is necessary to propose a centrifugal sample mixing device and a centrifugal sample mixing method.

[0004] In the first aspect, an embodiment of the present application provides a centrifugal sample mixing device, which includes: a carrying plate, a temperature raising and lowering module, a moving module and a centrifugal driving module, wherein the carrying plate is used to carry a centrifugal container, and the centrifugal container contains different fluids, wherein the moving module is used to drive the temperature raising and lowering module to move in a direction toward or away from the carrying plate so that the temperature raising and lowering module is attached to or separated from the centrifugal container; the centrifugal driving module is used to drive the carrying plate to rotate after the temperature raising and lowering module is separated from the carrying plate, so that different fluids located in the centrifugal container are mixed; the temperature raising and lowering module is used to heat and / or cool the fluid in the centrifugal container after being attached to the centrifugal container.

[0005] In some possible embodiments, the moving module includes a moving mechanism connected to the temperature raising and lowering module, and the moving mechanism is used to drive the temperature raising and lowering module to move; the centrifugal driving module includes a centrifugal driving mechanism connected to the carrying plate, and the centrifugal driving mechanism is used to drive the carrying plate to rotate.

[0006] In some possible embodiments, the centrifugal drive mechanism includes: a centrifugal motor, a centrifugal shaft, and a connecting assembly connecting the centrifugal motor and the centrifugal shaft, which are arranged on a base; the supporting plate includes a centrifugal turntable connected to an end of the centrifugal shaft away from the centrifugal motor, and a positioning structure arranged on the periphery of the centrifugal turntable, the centrifugal container is located on the centrifugal turntable, and the positioning structure is used to fix the centrifugal container.

[0007] In some possible embodiments, a plurality of positioning structures are provided on the periphery of the centrifugal turntable, and each of the positioning structures includes: a fixed block provided on the periphery of the centrifugal turntable, the fixed block having a slot facing the central axis of the centrifugal turntable, and a clamping block is provided on the periphery of the centrifugal container corresponding to the slot, and the fixed block is used to clamp the clamping block after the clamping block extends into the slot, so that the centrifugal container is fixed on the centrifugal turntable.

[0008] In some possible embodiments, the positioning structure further includes a fastener disposed along a central axis perpendicular to the centrifugal turntable, wherein the fastener penetrates the side wall of the fixing block and extends into the slot to abut against the side wall of the blocking block.

[0009] In some possible embodiments, the temperature rise and fall module includes a heat conduction plate, a heater and a radiator stacked in sequence, the heat conduction plate is located below the centrifugal turntable, and the centrifugal shaft passes through the heat conduction plate, the heater and the radiator and is connected to the centrifugal turntable.

[0010] In some possible embodiments, the heat conducting plate includes a main body and a raised portion located on a side of the main body close to the supporting plate, and a side of the centrifugal container close to the heat conducting plate has a recessed portion that cooperates with the raised portion, and the raised portion can extend into the recessed portion and fit with the centrifugal container.

[0011] In some possible embodiments, the moving mechanism includes: a moving motor disposed on the base, a telescopic shaft disposed at the output end of the moving motor, a push plate disposed at an end of the telescopic shaft away from the moving motor, a heat dissipation base plate located on the push plate and connected to the push plate, a support provided on the heat dissipation base plate, the radiator is fixed on the support, and the push plate and the heat dissipation base plate are connected via an elastic member;

[0012] The centrifugal sample mixing device also includes a first sensor group and a second sensor group. The first sensor group is used to sense whether the temperature raising and lowering module moves into place along the direction toward the supporting plate, and the second sensor group is used to sense whether the temperature raising and lowering module moves into place along the direction away from the supporting plate.

[0013] In a second aspect, an embodiment of the present application further provides a centrifugal sample mixing system, which comprises a centrifugal container and the centrifugal sample mixing device as described above.

[0014] In a third aspect, an embodiment of the present application further provides a method for centrifugal sample mixing using the centrifugal sample mixing system as described above, the method comprising:

[0015] Fixing the centrifuge container on the carrier plate;

[0016] The temperature-raising and cooling module is driven to move by a moving module, so that the temperature-raising and cooling module is separated from the carrying plate;

[0017] The centrifugal drive module drives the carrier plate to rotate, so that different fluids in the centrifugal container are centrifuged and mixed;

[0018] The moving module drives the temperature raising and lowering module to move, so that the temperature raising and lowering module fits with the centrifugal container; and

[0019] The fluid in the centrifuge container is heated and / or cooled by the temperature increasing and decreasing module.

[0020] The above-mentioned centrifugal sample mixing system provided in the embodiment of the present application adopts a centrifugal liquid exchange device through the mutual cooperation of a temperature rise and fall module, a mobile module and a centrifugal drive module, which can realize the mixing and centrifugation of different fluids in a centrifugal container, and can also realize the temperature rise and fall operation of the mixed fluid, wherein, the fluid mixing adopts a single-power centrifugal drive, and the mixing of different fluids can be realized through different rotation speeds, and at the same time, the temperature rise and fall module can be directly used to heat and fall the mixed fluid in the centrifugal container, and the centrifugal sample mixing and the temperature rise and fall operation do not interfere with each other. The centrifugal container can realize rotational motion and temperature rise and fall reaction in the centrifugal sample mixing device, the overall operation is simple, the process of multi-step liquid transfer is simplified, and the centrifugal and temperature rise and fall efficiency is high; moreover, the structure of the centrifugal sample mixing device is simple and compact, does not need to set up a variety of equipment, is small in size, and occupies a small space; during the centrifugal mixing and temperature rise and fall operation process, the liquid is always located inside the centrifugal container, and there is no problem of reagent contamination and reagent waste caused by liquid transfer, which improves the experimental success rate and reduces the cost at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments of the present invention will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0022] Figure 1Schematic diagram of a centrifugal sample mixing system in one embodiment of the present application.

[0023] Figure 2 It is a structural schematic diagram of a centrifugal sample mixing system in one embodiment of the present application.

[0024] Figure 3 yes Figure 2 Schematic diagram of the structure after the centrifugal sample mixing device is separated from the centrifugal container.

[0025] Figure 4 yes Figure 3 Enlarged view of the center carrier plate and centrifuge container.

[0026] Figure 5 yes Figure 2 Schematic diagram of the structure of the centrifugal drive mechanism.

[0027] Figure 6 yes Figure 5 Cross-section along line VI-VI.

[0028] Figure 7 yes Figure 2 Schematic diagram of the structure of the medium and low temperature heating and cooling module and the moving mechanism.

[0029] Figure 8 yes Figure 7 Exploded diagram.

[0030] Fig. 9 yes Figure 7 Section along line IX-IX.

[0031] Fig.10 Flow chart of the centrifugal mixing method in one embodiment of the present application.

[0032] Main component symbols

[0033]

[0034]

[0035] The following specific implementation manner will further illustrate the present invention in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] It should be noted that when a component is referred to as being "fixed to" or "mounted on" another component, it may be directly on the other component or there may be a central component. When a component is considered to be "set on" another component, it may be directly set on the other component or there may be a central component at the same time. The term "and / or" as used herein includes all and any combinations of one or more of the relevant listed items.

[0038] See also Figure 1 The embodiment of the present application provides a centrifugal sample mixing system 1000, the centrifugal sample mixing system 1000 includes a centrifugal sample mixing device 100 and a centrifugal container 200, the centrifugal sample mixing device 100 includes: a carrier plate 1, a temperature rise module 2, a moving module 30 and a centrifugal driving module 40, wherein the carrier plate 1 is used to carry the centrifugal container 200, and the centrifugal container 200 can be used to contain fluids, wherein the fluids can include different reagents and samples. The moving module 30 is used to drive the temperature rise module 2 to move toward or away from the carrier plate 1, so that the temperature rise module 2 is attached to or separated from the centrifugal container 200. After the temperature rise module 2 leaves the carrier plate 1, that is, after the temperature rise module 2 is separated from the centrifugal container 200, the centrifugal driving module 40 is used to drive the carrier plate 1 to rotate, so that the different fluids in the centrifugal container 200 are mixed. The temperature rise module 2 is used to heat and / or cool the mixed fluids in the centrifugal container 200 after being attached to the centrifugal container 200. Specifically, the centrifugal container 200 may have a plurality of microchannels and a mixing chamber connected to the plurality of microchannels. The plurality of microchannels may accommodate different reagents and samples. During the centrifugation process, the fluids in different microchannels may be added to the mixing chamber for mixing. Furthermore, after the centrifugal mixing, the mixed fluid may be subjected to temperature increase and decrease treatments to achieve the purpose of synchronous centrifugal mixing and temperature increase and decrease.

[0039] See also Figure 2 FIG. 1 is a schematic diagram of the specific structure of the centrifugal sample mixing system 1000. Figure 3As shown, it is a schematic diagram of the specific structure after the centrifugal sample mixing device 100 is separated from the centrifugal container 200. Among them, the temperature rise and fall module 2 is located below the carrier plate 1 and can realize the temperature rise and fall of the centrifugal container 200. The moving module 30 includes a moving mechanism 3 that can be connected to the temperature rise and fall module 2, and the moving mechanism 3 can drive the temperature rise and fall module 2 to move toward or away from the carrier plate 1, so as to realize the fitting or separation of the temperature rise and fall module 2 and the centrifugal container 200 located on the carrier plate 1. The centrifugal driving module 40 includes a centrifugal driving mechanism 4 that is rotatably connected to the carrier plate 1, and the centrifugal driving mechanism 4 can drive the carrier plate 1 to rotate to realize the centrifugal mixing of different reagents in the centrifugal container 200. Among them, during the centrifugation process, the temperature rise and fall module 2 needs to be separated from the centrifugal container 1, and during the temperature rise and fall process, the temperature rise and fall module 2 needs to fit with the centrifugal container 1.

[0040] It can be understood that the centrifugal sample mixing device 100 also includes a control module 8, and the control module 8 is used to control the centrifugal drive module 40, the temperature rise and fall module 2 and the moving module 30. The specific control method can be set according to actual needs, and this embodiment does not limit this.

[0041] Please refer again Figure 2 and Figure 3 The centrifugal sample mixing device 100 also includes a base 5, and the moving mechanism 3 and the centrifugal driving mechanism 4 are both fixed on the base 5, and the moving mechanism 3 and the centrifugal driving mechanism 4 are coaxially arranged.

[0042] In some embodiments, the base 5 includes a bottom plate 51 , and a first support frame 52 and a second support frame 53 disposed on the bottom plate 51 , wherein the centrifugal drive mechanism 4 is disposed on the first support frame 52 , and the moving mechanism 3 is disposed on the second support frame 53 .

[0043] See also Figure 3 The carrying plate 1 includes a centrifugal turntable 11 and a positioning structure 12 arranged on the periphery of the centrifugal turntable 11. The centrifugal turntable 11 is rotatably arranged at one end of the centrifugal driving mechanism 4. The centrifugal turntable 11 is used to carry a centrifugal container 200, and the positioning structure 12 is used to fix the centrifugal container 200 on the centrifugal turntable 11.

[0044] In some embodiments, a plurality of positioning structures 12 are provided on the periphery of the centrifugal turntable 11, and each positioning structure 12 includes: a fixed block 13 provided on the periphery of the centrifugal turntable 11, the fixed block 13 having a slot 131 facing the central axis of the centrifugal turntable 11, a block 201 is provided on the periphery of the centrifugal container 200 corresponding to the slot 131, and the block 201 can extend into the slot 131, and the fixed block 13 is used to clamp the block 201 so that the centrifugal container 200 is fixed on the centrifugal turntable 11. For example, when the centrifugal container 200 is roughly disc-shaped, the corresponding centrifugal turntable 11 is also disc-shaped, and a plurality of positioning structures 12 are arranged on the periphery of the centrifugal turntable 11. When the clamping block 201 on the centrifugal container 200 is clamped into the clamping groove 131 on the corresponding fixing block 13, the plurality of positioning structures 12 can clamp the centrifugal container 200 from different directions, so that the centrifugal container 200 is stably fixed on the centrifugal turntable 11, so as to prevent the centrifugal container 200 from accidentally detaching from the centrifugal turntable 11 during the centrifugation process, thereby improving reliability.

[0045] In some embodiments, the positioning structure 12 also includes a fastener 14 arranged along a central axis perpendicular to the centrifugal turntable 11, the fastener 14 passes through the side wall of the fixed block 13 and extends into the slot 131, and one end of the fastener 14 extending into the slot 131 abuts against the side wall of the block 201, thereby providing a clamping force for the centrifugal container 200 toward the central axis, further improving the stability of the centrifugal container 200 on the centrifugal turntable 11.

[0046] In some embodiments, reference is made to Figure 2 and Figure 3 The positioning structure 12 further includes a pressing block 15 rotatably connected to at least one end of the fixing block 13. An elastic plunger 16 is provided on the temperature-adding and cooling module 2 corresponding to the pressing block 15. The pressing block 15 has a groove with an opening toward the elastic plunger 16. After the temperature-adding and cooling module 2 moves to the carrier plate 1 and fits with the centrifugal container 200, the elastic plunger 16 extends into the groove so that one end of the pressing block 15 close to the centrifugal container 200 abuts against the upper surface of the centrifugal container 200. In some embodiments, the elastic plunger 16 is disposed on a pressing plate 17, which is fixed to the periphery of the temperature-adding and cooling module 2. In some embodiments, the elastic plunger 16 may be a spring plunger.

[0047] See also Figure 5 and Figure 6 , and refer to Figure 2 and Figure 3The centrifugal drive mechanism 4 includes: a centrifugal motor 41 disposed on the base 5, a centrifugal shaft 42 located at the output end of the centrifugal motor 41, and a connecting assembly 43 connecting the output end of the centrifugal motor 41 and the centrifugal shaft 42. The carrier plate 1 is connected to one end of the centrifugal shaft 42 away from the centrifugal motor 41. Specifically, the centrifugal motor 41 is fixed on the first support frame 52, and the centrifugal turntable 11 is connected to the centrifugal shaft 42. The centrifugal motor 41 can drive the centrifugal shaft 42 to rotate, and then drive the centrifugal turntable 11 to rotate, so as to realize the centrifugation of the fluid in the centrifugal container 200 located on the centrifugal turntable 11. Specifically, by controlling the rotation speed of the centrifugal turntable 11 and coordinating with the specific design of the microchannel, the order in which the fluids in different microchannels in the centrifugal container 200 are added to the mixing chamber can be controlled to achieve the mixing of multiple fluids.

[0048] In some embodiments, the connection assembly 43 includes a coupling 431, a locking nut 432, an angular contact bearing group 433 and a centrifugal sleeve 434. The coupling 431 is connected to the output end of the centrifugal motor 41, and the centrifugal shaft 42 is connected to the coupling 431. The locking nut 432 is a double nut, which is locked on the centrifugal shaft 42 and is located on the side of the coupling 431 away from the centrifugal motor 41. The angular contact bearing group 433 is sleeved on the centrifugal shaft 42 and is located on the side of the locking nut 432 away from the coupling 431. The centrifugal sleeve 434 is sleeved on the outer side of the locking nut 432 and the angular contact bearing group 433.

[0049] See also Figures 7 to 9 , and refer to Figure 2 and Figure 5 The temperature rise and fall module 2 includes a heat conduction plate 21, a heater 22 and a radiator 23 stacked in sequence, the heat conduction plate 21 is located below the carrier plate 1, and the centrifugal shaft 42 passes through the heat conduction plate 21, the heater 22 and the radiator 23 and is connected to the centrifugal turntable 11. It can be understood that the centrifugal drive mechanism 4 and the moving mechanism 3 move independently without interfering with each other, and the temperature rise and fall module 2 does not interfere with the centrifugal drive mechanism 4 during the movement process.

[0050] In some embodiments, the heat conducting plate 21 includes a body 211 and a protrusion 212 located on one side of the body 211 close to the carrier plate 1, and the centrifugal container 200 has a recessed portion 202 that cooperates with the protrusion 212 on one side of the heat conducting plate 21, and the protrusion 212 can extend into the recessed portion 202 and fit with the centrifugal container 200. By providing the protrusion 212 on the heat conducting plate 21 and extending into the recessed portion 202 of the centrifugal container 200 to fit with the centrifugal container 200, it is beneficial to increase the contact area and tightness of the fit. It can be understood that the centrifugal container 200 has the aforementioned mixing chamber at the location where the recessed portion 202 is provided, so that the protrusion 212 of the heat conducting plate 21 can directly increase or decrease the temperature of the mixing chamber of the centrifugal container 200, thereby achieving heating or cooling of the mixed sample in the mixing chamber.

[0051] In some embodiments, the heater 22 may be a semiconductor refrigerator (TEC), which can realize two temperature changing modes: heating and cooling. The heater 22 has a simple structure, which is conducive to reducing the volume of the overall centrifugal mixing device 100.

[0052] In some embodiments, see also Figure 4 As shown, the centrifugal turntable 11 is provided with a through hole 18 corresponding to the protrusion 212, and the protrusion 212 of the heat conducting plate 21 passes through the through hole 18 and extends into the recess 202 to fit with the centrifugal container 200. By providing the through hole 18 on the centrifugal turntable 11, it is beneficial for the protrusion 212 of the heat conducting plate 21 to directly contact with the centrifugal container 200, which is beneficial to improve the sensitivity of temperature rise and fall, improve the accuracy of temperature control and improve the efficiency of temperature rise and fall.

[0053] Please refer again Figures 7 to 9 , and refer to Figure 2 The moving mechanism 3 includes: a moving motor 31 arranged on the base 5, a telescopic shaft 32 arranged on the output end of the moving motor 31, a push plate 33 arranged at the end of the telescopic shaft 32 away from the moving motor 31, a heat dissipation base plate 34 located on and connected to the push plate 33, and a support 35 arranged on the heat dissipation base plate 34, and the radiator 23 is fixed on the support 35. That is, the temperature rise and fall module 2 is fixed on the heat dissipation base plate 34 through the support 35, and the moving motor 31 drives the telescopic shaft 32 to push and pull, thereby pushing the push plate 33 and the heat dissipation base plate 34 to move up and down, thereby realizing the up and down movement of the temperature rise and fall module 2, so as to approach or move away from the centrifugal turntable 11, and then realize the attachment or separation with the centrifugal container 200 on the centrifugal turntable 11. The movement of the moving mechanism 3 and the centrifugal driving mechanism 4 is relatively independent and will not interfere with each other. Therefore, the independent movement of the temperature rise and fall module 2 and the carrier plate 1 can be realized, thereby realizing the independent performance of the centrifugal mixing and temperature rise and fall processes.

[0054] In some embodiments, the push plate 33 and the heat dissipation bottom plate 34 are connected by an elastic member 36. Specifically, a plurality of elastic members 36 are arranged between the push plate 33 and the heat dissipation bottom plate 34, so as to prevent the temperature rise and fall module 2 from rigidly colliding with the centrifugal container 200 and causing damage. Moreover, after the temperature rise and fall module 2 is attached to the centrifugal container 200, the heat conduction plate 21 and the centrifugal container 200 can be flexibly attached, thereby improving the evenness of the attachment and reducing the problem of uneven force on the centrifugal container 200. In some embodiments, the elastic member 36 includes a guide post 361 arranged on the push plate 33 and penetrating the heat dissipation bottom plate 34, and a spring 362 sleeved on the guide post 361 and located between the push plate 33 and the heat dissipation bottom plate 34. The heat dissipation bottom plate 34 can slide along the guide post 361 to compress the spring 362.

[0055] In some embodiments, the moving mechanism 3 also includes a guide shaft 37 arranged on the side of the push plate 33 close to the base 5, and the base 5 is provided with a guide hole corresponding to the guide shaft 37. When the push plate 33 is driven up and down, the guide shaft 37 can move along the guide hole to improve the movement stability of the moving mechanism 3.

[0056] In some embodiments, Figure 2 and Figure 3 As shown, the centrifugal sample mixing device 100 also includes a first sensor group 6 and a second sensor group 7. The first sensor group 6 is used to sense whether the temperature rise and fall module 2 moves away from the carrier plate 1, that is, whether the temperature rise and fall module 2 is separated from the centrifugal container 200. The second sensor group 7 is used to sense whether the temperature rise and fall module 2 moves toward the carrier plate 1, that is, whether the temperature rise and fall module 2 is fitted with the centrifugal container 200. In some embodiments, the first sensor group 6 includes a first sensor sheet 61 located on the moving mechanism 3 and a first sensor 62 located on the base 5. The first sensor sheet 61 moves in a direction away from the carrier plate 1 under the drive of the moving mechanism 3. When the moving mechanism 3 drives the temperature rise and fall module 2 to move (for example, descend) to the position, the first sensor sheet 61 triggers the first sensor 62, so that the control module 8 can obtain a signal of separation, and then control the moving mechanism 3 to stop descending. The second sensor group 7 includes a second sensing sheet 71 located on the moving mechanism 3 and a second sensor 72 located on the base 5. The second sensing sheet 71 moves toward the supporting plate 1 driven by the moving mechanism 3. When the moving mechanism 3 drives the temperature rise and fall module 2 to move (for example, rise) into place, the second sensing sheet 71 triggers the second sensor 72, so that the control module 8 can obtain a signal indicating that it is in place, and then control the moving mechanism 3 to stop rising.

[0057] Please refer again Figure 2The centrifugal sample mixing device 100 further includes a housing 9 for accommodating the temperature rise and fall module 2, the moving mechanism 3 and the centrifugal drive mechanism 4, and the housing 9 has an opening 91, and the carrier plate 1 extends out of the opening 91 and is located outside the housing 9, which is convenient for the installation of the centrifugal container 200 and the connection between the centrifugal container 200 and the reagent kit (not shown), etc., wherein the reagent kit is a device for holding different reagents and samples, and corresponding reagents or samples can be added to the microchannel of the centrifugal container 200. A heat dissipation fan 92 is also installed on the housing 9 to accelerate the heat dissipation inside the housing 9.

[0058] See also Fig.10 , and refer to Figures 1 to 3 The present application also provides a method for centrifugal sample mixing using the aforementioned centrifugal sample mixing system 1000, the method specifically comprising the following steps:

[0059] Step S1 , fixing the centrifugal container 200 on the supporting plate 1 of the centrifugal sample mixing device 100 .

[0060] Specifically, after the centrifugal container 200 is fixed on the carrier plate 1, the centrifugal container 200 is connected to the reagent kit (not shown), and fluids such as reagents or samples are added to the microfluidic channels of the centrifugal container 200. It is understandable that the centrifugal container 200 can also be loaded with fluids such as reagents or samples in advance, and then the centrifugal container 200 is fixed on the carrier plate 1.

[0061] Step S2 , driving the temperature-raising and cooling module 2 to move by the moving module 30 , so as to separate the temperature-raising and cooling module 2 from the carrying plate 1 .

[0062] Before centrifugation, the temperature-adding and cooling module 2 cannot contact the centrifugal container 200, and the moving mechanism 3 in the moving module 30 drives the temperature-adding and cooling module 2 to move downward, and moves the temperature-adding and cooling module 2 away from the carrier plate 1. This can prevent the temperature-adding and cooling module 2 from causing interference during the centrifugation process, and the temperature-adding and cooling module 2 will not rotate at the same time during the centrifugation process, thereby reducing the complexity of the structure, and also preventing damage to the temperature-adding and cooling module 2, thereby increasing the service life.

[0063] In step S3, the centrifugal driving module 40 drives the carrier plate 1 to rotate, so as to drive the centrifugal container 200 to rotate, so as to centrifugally mix different fluids in the centrifugal container 200.

[0064] The centrifugal drive mechanism 4 in the centrifugal drive module 40 drives the carrier plate 1 to rotate, thereby achieving centrifugal mixing of different reagents and samples in the centrifugal container 200. Specifically, during the centrifugation process, different reagents and samples in different microchannels in the centrifugal container 200 flow into the mixing chamber, and the different reagents and samples in the mixing chamber are evenly mixed during the centrifugation process.

[0065] Step S4 , the moving module 30 drives the temperature-adding and cooling module 2 to move toward the supporting plate 1 , so that the temperature-adding and cooling module 2 fits the centrifugal container 200 .

[0066] When the moving mechanism 3 in the moving module 30 drives the temperature-adding and cooling module 2 to move upward, the elastic member 36 exists between the push plate 33 in the moving mechanism 3 and the heat dissipation bottom plate 34, so that the temperature-adding and cooling module 2 and the centrifugal container 200 can be flexibly fitted, and direct hard collision and contact between them can also be avoided. In addition, when the moving mechanism 3 pushes the temperature-adding and cooling module 2 to move upward, the elastic plunger 16 located on the temperature-adding and cooling module 2 will extend into the pressing block 15 located on the carrier plate 1, so that the pressing block 15 presses the centrifugal container 200 from top to bottom.

[0067] Step S5, heating and / or cooling the fluid in the centrifugal container 200 by the temperature increasing and decreasing module 2.

[0068] Specifically, the temperature increasing and decreasing module 2 can be started after the fluids are mixed, and the temperature increasing and decreasing module 2 can be started to heat only the mixed fluid in the mixing chamber in the centrifugal container 200, or only cool the mixed fluid. It is understandable that the temperature increasing and decreasing module 2 can also be started before the fluids are mixed, and the reagents or samples are heated first, and then centrifugally mixed.

[0069] It is understandable that the above steps S2 to 5 can be repeated according to the experimental needs to perform mixing of different reagents and temperature rise and fall reactions. The actual working sequence is not limited to the above steps, and the order of temperature control and centrifugal mixing can be adjusted according to the needs. The centrifugal container 200 is not limited to rotating in one direction, and it can also rotate back and forth in the forward and reverse directions as needed.

[0070] In summary, the centrifugal sample mixing system 1000 provided in the embodiment of the present application adopts the centrifugal sample mixing device 100 to cooperate with the heating and cooling module 2, the moving module 30 and the centrifugal drive module 40 to achieve the mixing and centrifugation of different fluids in the centrifugal container 200, and the heating and cooling operation of the mixed fluid can be realized at the same time, wherein the reagent mixing adopts a single power centrifugal drive, and the mixing of different fluids can be achieved through different speeds. At the same time, the heating and cooling module 2 can be directly used to heat and cool the mixed fluid in the centrifugal container 200, and the centrifugal sample mixing and the heating and cooling operations do not interfere with each other. The centrifugal container 200 can realize rotational motion and heating and cooling reactions in the centrifugal sample mixing device 100, the overall operation is simple, the process of multi-step liquid transfer is simplified, and the centrifugal and heating and cooling efficiency is high; moreover, the structure of the centrifugal sample mixing device 100 is simple and compact, does not need to set up a variety of equipment, is small in size, and occupies little space; during the centrifugal mixing and heating and cooling operations, the liquid is always located inside the centrifugal container 200, and there is no problem of reagent contamination and reagent waste caused by liquid transfer, which improves the experimental success rate and reduces the cost at the same time.

[0071] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A centrifugal sample mixing device, characterized in that: include: A carrier plate, a temperature rise and fall module, a moving module and a centrifugal drive module. The carrier plate is used to carry a centrifugal container. Different fluids are contained in the centrifugal container. Among them, the moving module is used to drive the temperature raising and lowering module to move in the direction toward or away from the carrying plate, so that the temperature raising and lowering module is attached to or separated from the centrifugal container; the centrifugal driving module is used to drive the carrying plate to rotate after the temperature raising and lowering module is separated from the carrying plate, so that different fluids located in the centrifugal container are mixed; the temperature raising and lowering module is used to heat and / or cool the fluid in the centrifugal container after being attached to the centrifugal container.

2. The centrifugal sample mixing device according to claim 1, characterized in that: The moving module includes a moving mechanism connected to the temperature raising and lowering module, and the moving mechanism is used to drive the temperature raising and lowering module to move; The centrifugal driving module includes a centrifugal driving mechanism connected to the carrying plate, and the centrifugal driving mechanism is used to drive the carrying plate to rotate.

3. The centrifugal sample mixing device according to claim 2, characterized in that: The centrifugal drive mechanism comprises: a centrifugal motor arranged on a base, a centrifugal rotating shaft, and a connecting assembly connecting the centrifugal motor and the centrifugal rotating shaft; The carrier plate comprises a centrifugal turntable connected to one end of the centrifugal shaft away from the centrifugal motor and a positioning structure arranged on the periphery of the centrifugal turntable. The centrifugal container is located on the centrifugal turntable, and the positioning structure is used to fix the centrifugal container.

4. The centrifugal sample mixing device according to claim 3, characterized in that: A plurality of positioning structures are provided on the periphery of the centrifugal turntable, each of the positioning structures comprises: a fixed block provided on the periphery of the centrifugal turntable, the fixed block having a clamping groove facing the central axis of the centrifugal turntable, a clamping block is provided on the periphery of the centrifugal container corresponding to the clamping groove, and the fixed block is used to clamp the clamping block after the clamping block extends into the clamping groove, so that the centrifugal container is fixed on the centrifugal turntable.

5. The centrifugal sample mixing device according to claim 4, characterized in that: The positioning structure also includes a clamping block rotatably connected to at least one end of the fixed block, the temperature raising and lowering module is provided with an elastic plunger corresponding to the clamping block, the clamping block has a groove opening toward the elastic plunger, and after the temperature raising and lowering module is fitted with the centrifugal container, the elastic plunger extends into the groove so that the end of the clamping block close to the centrifugal container is pressed against the upper surface of the centrifugal container.

6. The centrifugal sample mixing device according to claim 3, characterized in that: The temperature rise and fall module comprises a heat conduction plate, a heater and a radiator which are stacked in sequence. The heat conduction plate is located below the centrifugal turntable. The centrifugal shaft passes through the heat conduction plate, the heater and the radiator and is connected to the centrifugal turntable.

7. The centrifugal sample mixing device according to claim 6, characterized in that: The heat conducting plate comprises a main body and a raised portion located on a side of the main body close to the carrying plate. The centrifugal container has a recessed portion cooperating with the raised portion on a side close to the heat conducting plate. The raised portion can extend into the recessed portion and fit the centrifugal container.

8. The centrifugal sample mixing device according to claim 6, characterized in that: The moving mechanism comprises: a moving motor arranged on the base, a telescopic shaft arranged at the output end of the moving motor, a push plate arranged at the end of the telescopic shaft away from the moving motor, a heat dissipation bottom plate located on the push plate and connected to the push plate, a support arranged on the heat dissipation bottom plate, the radiator is fixed on the support, and the push plate and the heat dissipation bottom plate are connected by an elastic member; The centrifugal sample mixing device also includes a first sensor group and a second sensor group. The first sensor group is used to sense whether the temperature raising and lowering module moves into place along the direction toward the supporting plate, and the second sensor group is used to sense whether the temperature raising and lowering module moves into place along the direction away from the supporting plate.

9. A centrifugal sample mixing system, characterized in that: It comprises a centrifugal container and the centrifugal sample mixing device as claimed in any one of claims 1 to 8.

10. A method for centrifugal sample mixing using the centrifugal sample mixing system according to claim 9, characterized in that: include: Fixing the centrifuge container on the carrier plate; The temperature-raising and cooling module is driven to move by a moving module, so that the temperature-raising and cooling module is separated from the carrying plate; The centrifugal drive module drives the carrier plate to rotate, so that different fluids in the centrifugal container are centrifuged and mixed; The moving module drives the temperature raising and lowering module to move, so that the temperature raising and lowering module fits with the centrifugal container; as well as The fluid in the centrifuge container is heated and / or cooled by the temperature increasing and decreasing module.

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