Intelligent micro-fluidic living cell culture observation and analysis equipment

Abstract

The invention discloses intelligent micro-fluidic living cell culture observation and analysis equipment, and relates to the technical field of cell culture.The intelligent micro-fluidic living cell culture observation and analysis equipment comprises a base body, the base body is provided with a hollow cavity, a chip assembly is installed in the cavity, and the front section and the rear section of the base body are provided with a sample inlet pipe and a sample outlet pipe which are communicated with the chip assembly respectively; a culture chamber is arranged in the middle of the body, at least two sample introduction substrates are arranged at one end part of the body, and at least two sample discharge substrates are arranged at the other end part of the body. The valve rod disclosed by the invention solves the problem that a stable growth environment of cells cannot be provided in the prior art, and effectively controls influence factors such as air pressure, perfusion and temperature in a cell culture process.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to an intelligent microfluidic living cell culture observation and analysis device. Background Art

[0002] Traditional cell culture is usually carried out in a cell culture incubator environment, which is a closed environment and cannot effectively observe the cultured cells. The cell culture process is mostly static culture, and dynamic culture cannot meet the simulation of near-physiological conditions for cell culture status, and cell performance parameters are greatly different from the actual state. Traditional cell culture platforms cannot collect and record various system parameters that adjust the cell culture status, and cannot integrate and analyze various parameters and record feedback.

[0003] In order to solve the above problems, the prior art provides many solutions. For example, the prior art US18678112 discloses a microfluidic device, which includes a microfluidic chip for producing micro organic spheres (MOS). The first channel is defined in the surface of the microfluidic chip and includes: a droplet generating part, the droplet generating part includes an inlet part, a junction between the inlet part and the emulsified fluid channel, and a chamber downstream of the junction. The cross-sectional area of ​​the chamber is larger than the cross-sectional area of ​​the inlet part. The first channel includes a polymerization part downstream of the droplet generating part, and the polymerization part has a serpentine structure. The device includes a barrel for MOS demulsification, and the barrel includes: a collection container; a substrate disposed on the collection container, and a membrane disposed between the collection container and the surface of the substrate. The second channel is defined in the surface of the substrate facing the collection container and is connected to the output fluid of the polymerization part of the first channel, but the monitoring scheme of the device for cell culture is not comprehensive enough. In this regard, the inventor believes that there is still room for improvement. Summary of the invention

[0004] The purpose of the present invention is to provide an intelligent microfluidic living cell culture observation and analysis equipment to solve the problem that the prior art cannot provide a stable cell growth environment and effectively control the influencing factors such as air pressure, perfusion, and temperature during the cell culture process.

[0005] In order to solve the above technical problems, the present invention specifically provides the following technical solutions: an intelligent microfluidic living cell culture observation and analysis equipment, including a substrate, the substrate is provided with a hollow chamber, a chip assembly is installed in the chamber, the front and rear sections of the substrate are respectively provided with an inlet tube and a sample outlet tube connected to the chip assembly, the chip assembly has a body, a culture chamber is provided in the middle of the body, at least two inlet substrates are provided at one end of the body, and at least two sample outlet substrates are provided at the other end of the body.

[0006] The present invention can intuitively observe the cell growth status inside the culture chamber from the upper part thereof by setting a chip component, so that the environmental factors required by the cells can be regulated according to the cell growth status, thereby realizing long-term cell culture and observation. Furthermore, the present invention respectively sets at least two sample introduction substrates and sample outlet substrates at both ends of the main body to realize multi-point sample introduction and outlet, so that the culture liquid or buffer solution can be introduced into the culture chamber in different regions, and the culture liquid or buffer solution can also be sent out in different regions. In this way, regional controlled cell culture can be realized inside the culture chamber, and this culture method does not need to set multiple input and output devices at the end of the chip component, thus saving the number of liquid inlet and liquid discharge devices, and can also solve the problem of different inlet and outlet powers of different devices.

[0007] When regional culture is required, the target culture region can be selected by blocking any sample inlet substrate and sample outlet substrate.

[0008] The chip component of the substrate of the present invention is a microfluidic chip, and a software control system for controlling the microfluidic chip is provided on the substrate. At the same time, an irrigation system, a temperature control system, a gas-liquid exchange system, a sensor system and a microscopic imaging system are also provided on the substrate, which are used to realize the characteristics of dynamic control of cell culture state, real-time feedback regulation and stable environmental conditions. It supports microscopic imaging and real-time observation and recording of the cell state in the microfluidic chip, and long-term cultivation of living cells; it can monitor the fluid state, gas content and component state, and biochemical state in the microfluidic chip in real time, and record and integrate and analyze various parameters. The system parameters can be adjusted in real time according to image observation combined with system parameter analysis to ensure a stable and reliable growth environment for the cells and avoid the influence of temperature, humidity, air pressure and biochemical state on the cells.

[0009] According to one embodiment of the present invention, the base body is provided with a first through hole respectively connected to the sample inlet tube and the sample outlet tube, the first through hole is connected to the chamber, and a sealing rubber ring is provided at the connection between the first through hole and the sample inlet tube and the sample outlet tube to prevent medium leakage. At the same time, the first through hole can be provided with a sensor inside thereof to obtain medium flow, temperature and other data and cooperate with sensors at other positions to form a sensor system, so that the entire cell culture process is monitored by sensors to adjust the culture data in time to stabilize the cell culture environment.

[0010] According to one embodiment of the present invention, a first flow groove is provided on the sample injection substrate, a first through hole connected in the direction of the sample injection tube is connected to the first flow groove and a sealing ring and other structural parts are provided at the connection point to prevent medium leakage, the first flow groove is connected to the culture chamber, the first flow groove has a bending section, the first flow groove is provided to realize the input of the medium into the culture chamber, and to realize the purpose of dividing the culture chamber for regional culture by controlling the first flow groove to be located at the outlet position of the culture chamber, of course, the medium can also be input into multiple first flow grooves at the same time to realize the culture chamber without regional difference culture, and the specific method can be based on The culture method is selected according to the experimental purpose. A bending section structure is set for the first flow groove to promote the medium flowing through it to flow on the bent path of the first flow groove. The bending section is used to intercept lumps that may exist in the medium. If lumps appear, their flow can be blocked at the bend of the first flow groove with a high probability to prevent larger lumps from entering the culture chamber and affecting cell culture. In this way, the filtering effect is achieved. After the lumps are intercepted, the blockage results can be judged by visual inspection and timely processing can be carried out. Of course, in this case, a sensor is provided inside the first through hole, and the blockage results can be obtained in time through sensor feedback.

[0011] According to one embodiment of the present invention, the sample outlet substrate is provided with a second flow groove, the first through hole connected in the direction of the sample outlet tube is connected to the second flow groove and a sealing ring and other structural parts are provided at the connection point to prevent medium leakage, the second through groove is connected to the culture chamber, and the second through groove has a bending section. The second flow groove is provided to realize the output of the medium to the inside of the culture chamber, and to realize the purpose of dividing the culture chamber for regional culture by controlling the second flow groove to be located at the inlet position of the culture chamber. Of course, the medium can also be output to multiple second flow grooves at the same time to realize culture without regional difference in the culture chamber. The specific culture method can be selected according to the experimental purpose. The second flow groove is provided with a bending section structure to cause the medium flowing through it to flow on the bent second flow groove diameter, and the bending section is used to intercept lumps that may exist in the medium. If lumps appear, their flow can be blocked with a high probability at the bending point of the second flow groove to prevent larger lumps from entering the culture chamber and affecting cell culture. In this way, the filtering effect is achieved and the cell culture status inside the reaction culture chamber, whether mutations occur, etc. After the lumps are intercepted, the blockage results can be judged by visual inspection and timely processing can be carried out. Of course, in this case, a sensor is provided inside the first through hole, and the blockage results can be obtained in time through sensor feedback.

[0012] According to one embodiment of the present invention, a first flow channel is provided on both sides of the culture chamber, and the body has a connecting channel connecting the first flow channel and the culture chamber, and a baffle column is arranged between the arrays in the connecting channel, and an arc-shaped baffle plate is arranged around the side of the baffle column. The baffle plates on adjacent baffle columns have a spacing distance or abutment. The present invention solves the problem that the culture chamber has only front and rear ports, resulting in a large medium pressure at its front and rear ports, which is unfriendly to the cells cultured at the front and rear ports of the culture chamber by arranging the first flow channel and the connecting channel with the culture chamber on both sides of the culture chamber. For this reason, the first flow channel and the connecting channel with the culture chamber can be used to enter the culture chamber from different directions. Liquid and air intake, so as to regulate the air pressure, temperature and medium flow rate inside the culture chamber, etc. For this reason, the internal cell culture area range is further controlled by the multi-directional medium input / output scheme of the culture chamber, so as to solve the problem that the cell propagation area is concentrated in an uncontrollable position. On the basis of the above scheme, a baffle column and a baffle plate are further arranged to control the flow rate of the medium entering the culture chamber, and the medium entering the culture chamber controls the range of the cell propagation area by controlling the flow rate.

[0013] According to one embodiment of the present invention, a film is arranged in the communication channel between the first flow channel and the culture chamber. The film is a PDMS elastic film material, and the permeable PDMS elastic film material is used to realize multi-directional medium input / output to the culture chamber.

[0014] According to one embodiment of the present invention, a first inlet is provided at one end of the first flow channel, and a first outlet is provided at the other end thereof, for enabling the entry and discharge of the medium.

[0015] According to one embodiment of the present invention, an air outlet head is arranged in the hollow chamber of the substrate, the air outlet head has an air outlet hole, the air outlet head is a frustum-shaped structure, the air outlet hole is arranged at the center of the air outlet head, and a guide surface is arranged around the outside of the air outlet head, and the guide surface and the frustum surface of the air outlet head have an inclined angle. The air outlet head is arranged in the substrate to control the temperature of the air flow input into the hollow chamber of the substrate. The air outlet head is arranged below the chip assembly, so as to avoid the outlet air flow directly blowing the chip assembly to affect the cultured cells. Further, by arranging the guide surface around the air outlet head, the effect of driving the flow of the gas around the air outlet head after the air flow is discharged from the air outlet hole is strengthened, so that the gas at the bottom of the substrate forms a flow in the hollow chamber to solve the problem of the temperature difference in the area inside the substrate causing the temperature control part to be accurate.

[0016] According to one embodiment of the present invention, support columns are arranged in an array at the bottom of the base to space the base from the bottom and avoid the influence of the bottom on the base, such as the humidity problem under the base caused by excessive air humidity at the bottom. At the same time, the support columns can adjust the horizontal height and inclination of the base to meet different horizontal requirements.

[0017] According to one embodiment of the present invention, a cover plate is provided above the hollow chamber of the substrate for covering the upper part of the culture chamber, so that the temperature inside the culture chamber can be accurately monitored and foreign objects can be prevented from entering the culture chamber.

[0018] Compared with the prior art, the present invention has the following beneficial effects: the present invention can intuitively observe the cell growth status inside the culture chamber from the upper part thereof by setting the chip assembly scheme, so that the environmental factors required by the cells can be regulated according to the cell growth status, thereby realizing long-term cell culture and observation. The base is also provided with an irrigation system, a temperature control system, a gas-liquid exchange system, a sensor system and a microscopic imaging system, which are used to realize the characteristics of dynamic control of cell culture status, real-time feedback regulation and stable environmental conditions. It supports microscopic imaging and real-time observation and recording of the cell status in the microfluidic chip, and long-term living cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0020] Figure 1 A schematic diagram of an intelligent microfluidic living cell culture observation and analysis equipment of the present invention; Figure 2 It is a schematic diagram of the chip assembly scheme of the present invention; Figure 3 It is a schematic diagram of the auxiliary component structure scheme of the present invention; Figure 4 This is a schematic diagram of another solution of the chip assembly of the present invention; Figure 5 This is a schematic diagram of the state of the base body having a built-in air outlet head of the present invention; Figure 6 It is a schematic diagram of the air outlet head solution of the present invention; Figure 7 This is a control system diagram of an intelligent microfluidic living cell culture observation and analysis equipment of the present invention.

[0021] Description of reference numerals: 10. Base; 11. Sample inlet tube; 12. Sample outlet tube; 13. Support column; 14. First through hole; 20. Chip assembly; 21. Sample inlet substrate; 22. Main body; 23. Culture chamber; 24. Sample outlet substrate; 30. Cover plate; 40. Auxiliary part; 41. Auxiliary plate; 42. First column; 43. Second column; 44. First spring; 50. Baffle column; 51. Baffle plate; 60. First flow channel; 61. First inlet; 62. First outlet; 70. Air outlet head; 71. Air outlet hole; 72. Guide surface. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] The following first describes the concepts involved in the present application in conjunction with the accompanying drawings. It should be noted that the following description of each concept is only to make the content of the present application easier to understand, and does not limit the scope of protection of the present application; at the same time, the embodiments and features in the embodiments of the present application can be combined with each other in the absence of conflict. The present application will be described in detail with reference to the accompanying drawings and in conjunction with the embodiments.

[0024] Embodiment 1 like Figure 1-7 As shown, the present invention provides an intelligent microfluidic living cell culture observation and analysis equipment, including a substrate 10, the substrate 10 is provided with a hollow chamber, a chip assembly 20 is installed in the chamber, the front and rear sections of the substrate 10 are respectively provided with a sample injection tube 11 and a sample output tube 12 connected to the chip assembly 20, the chip assembly 20 has a body 22, a culture chamber 23 is provided in the middle of the body 22, at least two sample injection substrates 21 are provided at one end of the body 22, and at least two sample output substrates 24 are provided at the other end of the body 22.

[0025] The present invention can intuitively observe the cell growth conditions inside the culture chamber 23 from the upper part thereof by setting up the chip component 20, so that the environmental factors required by the cells can be regulated according to the cell growth conditions, thereby realizing long-term cell culture and observation. Furthermore, the present invention respectively sets at least two sample introduction substrates 21 and sample outlet substrates 24 at both ends of the main body 22 to realize multi-point sample introduction and outlet, so that the culture liquid or buffer solution can be introduced into the culture chamber 23 in different regions, and the culture liquid or buffer solution can also be sent out in different regions. In this way, regional controlled cell culture can be realized in the culture chamber 23, and this culture method does not need to set up multiple input and output devices at the end of the chip component 20, thus saving the number of liquid inlet and outlet devices, and can also solve the problem of different inlet and outlet powers of different devices.

[0026] When regional culture is required, the target culture region can be selected by blocking any one of the sample input substrate 21 and the sample output substrate 24 .

[0027] As shown in the float 7, the chip assembly 20 of the substrate 10 of the present invention is a microfluidic chip. The substrate 10 is provided with a software control system for controlling the microfluidic chip. At the same time, the substrate 10 is also provided with an irrigation system, a temperature control system, a gas-liquid exchange system, a sensor system and a microscopic imaging system, which are used to realize the characteristics of dynamic control of cell culture state, real-time feedback regulation and stable environmental conditions. It supports microscopic imaging and real-time observation and recording of the cell state in the microfluidic chip, and long-term cultivation of living cells; it can monitor the fluid state, gas content and component state, and biochemical state in the microfluidic chip in real time, and record and integrate and analyze various parameters. According to image observation combined with system parameter analysis, the system parameters can be adjusted in real time to ensure a stable and reliable growth environment for the cells, and avoid the influence of temperature, humidity, air pressure and biochemical state on the cells.

[0028] The base body 10 is provided with a first through hole 14 respectively connected with the sample inlet tube 11 and the sample outlet tube 12. The first through hole 14 is connected with the chamber. A sealing rubber ring is provided at the connection between the first through hole 14 and the sample inlet tube 11 and the sample outlet tube 12 to prevent medium leakage. At the same time, the first through hole 14 can be provided with a sensor inside thereof to obtain medium flow, temperature and other data and cooperate with sensors at other positions to form a sensor system. In this way, the entire cell culture process is monitored by sensors to adjust the culture data in time to stabilize the cell culture environment.

[0029] The sample injection substrate 21 is provided with a first flow groove, the first through hole 14 connected to the sample injection tube 11 is connected to the first flow groove, and a sealing ring and other structural parts are provided at the connection point to prevent medium leakage. The first flow groove is connected to the culture chamber 23, and the first flow groove has a bending section. The first flow groove is provided to realize the input of the medium into the culture chamber 23, and to realize the purpose of dividing the culture chamber 23 for regional culture by controlling the first flow groove to be located at the outlet position of the culture chamber 23. Of course, the medium can also be input into multiple first flow grooves at the same time to realize the culture chamber 23 without regional difference culture. The culture method can be selected according to the purpose of the experiment. A bending section structure is set for the first flow groove to promote the medium flowing through it to flow on the bent path of the first flow groove. The bending section is used to intercept lumps that may exist in the medium. If lumps appear, their flow can be blocked at the bend of the first flow groove with a high probability to prevent larger lumps from entering the culture chamber and affecting cell culture. In this way, the filtering effect is achieved. After the lumps are intercepted, the blockage results can be judged by visual inspection and timely processing can be carried out. Of course, in this case, a sensor is provided inside the first through hole 14, and the blockage results can be obtained in time through sensor feedback.

[0030] The sample outlet substrate 24 is provided with a second flow groove, the first through hole 14 connected to the sample outlet tube 12 is connected to the second flow groove and a sealing ring and other structural parts are provided at the connection point to prevent medium leakage. The second through groove is connected to the culture chamber 23 and has a bending section. The second flow groove is provided to realize the output of the medium to the inside of the culture chamber 23, and to realize the purpose of dividing the culture chamber 23 for regional culture by controlling the second flow groove to be located at the inlet position of the culture chamber 23. Of course, the medium can also be output to multiple second flow grooves at the same time to realize the culture chamber 23 without regional difference. The culture method can be selected according to the experimental purpose. The second flow groove is provided with a bending section structure to prompt the medium flowing through it to flow on the bent second flow groove diameter, and the bending section is used to intercept the lumps that may exist in the medium. If lumps appear, their flow can be blocked with a high probability at the bending point of the second flow groove to prevent larger lumps from entering the culture chamber and affecting the cell culture. In this way, the filtering effect is achieved and the cell culture status inside the reaction culture chamber 23, whether mutations occur, etc. After the lumps are intercepted, the blockage results can be judged by visual inspection and timely processing can be carried out. Of course, in this case, a sensor is provided inside the first through hole 14, and the blockage results can be obtained in time through sensor feedback.

[0031] The first flow channel 60 is provided on both sides of the culture chamber 23, and the body 22 has a connecting channel connecting the first flow channel 60 and the culture chamber 23. The connecting channel is provided with flow blocking columns 50 in arrays, and arc-shaped flow blocking plates 51 are arranged around the sides of the flow blocking columns 50. The flow blocking plates 51 on adjacent flow blocking columns 50 are spaced apart or abutted. The present invention solves the problem by setting a first flow channel 60 and a channel connecting to the culture chamber 23 on both sides of the culture chamber 23. The culture chamber 23 has only front and rear ports, which leads to a relatively high medium pressure at the front and rear ports, which is not friendly to the cells cultured at the front and rear ports of the culture chamber 23. Therefore, the first flow channel 60 and the channel connecting to the culture chamber 23 can allow liquid and air to enter the culture chamber 23 from different directions to regulate the air pressure, temperature and medium flow rate inside the culture chamber 23. Therefore, the range of the internal cell culture area is further controlled by a multi-directional medium input / output scheme for the culture chamber 23 to solve the problem that the cell propagation area is concentrated in an uncontrollable position. On the basis of the above scheme, a baffle column 50 and a baffle plate 51 are further set to control the flow rate of the medium entering the culture chamber 23, so as to control the range of the cell propagation area by controlling the flow rate of the medium entering the culture chamber 23.

[0032] A film is arranged in the communication channel between the first flow channel 60 and the culture chamber 23. The film is made of a PDMS elastic film material, and the permeable PDMS elastic film material is used to realize multi-directional medium input / output to the culture chamber 23.

[0033] The first flow channel 60 is provided with a first inlet 61 at one end and a first outlet 62 at the other end for enabling the entry and exit of the medium.

[0034] An air outlet head 70 is arranged in the hollow chamber of the substrate 10. The air outlet head 70 has an air outlet hole 71. The air outlet head 70 is a frustum-shaped structure. The air outlet hole 71 is arranged at the center of the air outlet head 70. A guide surface 72 is arranged around the outside of the air outlet head 70. The guide surface 72 and the frustum surface of the air outlet head 70 have an inclined angle. The air outlet head 70 is arranged in the substrate 10 to control the temperature of the air flow input to the hollow chamber of the substrate 10. The air outlet head 70 is arranged below the chip assembly 20, so as to avoid the air flow from blowing directly on the chip assembly 20 to affect the cultured cells. Further, by arranging the guide surface 72 around the air outlet head 70, the air flow is strengthened to drive the gas around the air outlet head 70 after being discharged from the air outlet hole 71. In this way, the gas at the bottom of the substrate 10 flows in the hollow chamber to solve the problem of the temperature difference in the area inside the substrate 10 causing the temperature control part to be accurate.

[0035] The base 10 is provided with support columns 13 in an array at the bottom thereof, so as to space the base 10 from the bottom and avoid the influence of the bottom on the base, such as the humidity problem under the base caused by excessive air humidity at the bottom. At the same time, the support columns 13 can adjust the horizontal height and inclination of the base 10 to meet different horizontal requirements.

[0036] According to one embodiment of the present invention, a cover plate 30 is provided above the hollow chamber of the base 10 for covering the upper part of the culture chamber 23, so that the temperature inside the culture chamber 23 can be accurately monitored and foreign objects can be prevented from entering the culture chamber.

[0037] Embodiment 2: This embodiment further optimizes the solution based on embodiment 1 as follows: Figure 1 , 2 As described in , 3, auxiliary parts 40 are arranged in an array in the culture chamber 23, and the auxiliary parts 40 have a first column 42 vertically connected to the culture chamber 23, preferably a threaded connection, and a second column 43 with the same structure is provided on one side of the first column 42, and the first column 42 and the second column 43 are provided with the same auxiliary plate 41, and the auxiliary plate 41 has a hole that is slidably connected to the first column 42, and the auxiliary plate 41 has a hole that is slidably connected to the second column 43.

[0038] The auxiliary plate 41 can move up and down relative to the first column 42 and the second column 43 . A first spring 44 is disposed on the first column 42 below the auxiliary plate 41 , and another first spring 44 is disposed on the second column 43 above the auxiliary plate 41 .

[0039] The upper ends of the first column 42 and the second column 43 are provided with limiting blocks for limiting the auxiliary plate 41 from being separated therefrom.

[0040] One end of the first spring 44 on the second column 43 abuts against the stopper on the second column 43, and the other end abuts against the upper surface of the auxiliary plate 41. One end of the first spring 44 on the first column 42 abuts against the lower surface of the auxiliary plate 41, and the other end abuts against the bottom surface of the hollow chamber of the body 22.

[0041] By placing the auxiliary component 40 inside the culture chamber 23, the auxiliary plate 41 of the auxiliary component 40 can be used to control the flow rate of the medium flowing through. After the medium flows through the auxiliary plate 41, the auxiliary plate 41 is pushed to move up and down relative to the first column 42 and the second column 43 according to the flow rate of the medium to consume the flow rate of the medium around the auxiliary plate 41, thereby solving the problem of uneven distribution of cell culture areas in the middle area of ​​the culture chamber 23 due to the impact of the culture fluid, etc. In addition, the auxiliary component 40 is arranged inside the culture chamber 23 to support the objects covered on the upper part of the culture chamber 23. For example, when a transparent plate is placed on the upper part of the culture chamber 23, the problem of the downward displacement and tilt of the middle part of the plate can be avoided, and the auxiliary component 40 can be used as an auxiliary reference for the cell culture area in the culture chamber.

[0042] The embodiments and / or implementation methods described above are only used to illustrate the preferred embodiments and / or implementation methods for realizing the technology of the present invention, and are not intended to limit the implementation methods of the technology of the present invention in any form. Any technical personnel in this field may make slight changes or modifications to other equivalent embodiments without departing from the scope of the technical means disclosed in the content of the present invention, but they should still be regarded as technologies or embodiments that are essentially the same as the present invention.

[0043] This article uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only used to help understand the method and its core ideas of this application. The above is only the preferred implementation method of this application. It should be pointed out that due to the limitations of textual expression and the objective existence of infinite specific structures, ordinary technicians in this technical field can make several improvements, modifications or changes without departing from the principles of this application, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the concept and technical solution of the invention to other occasions without improvement, should be regarded as the scope of protection of this application.

Claims

1. An intelligent microfluidic living cell culture observation and analysis device, comprising a substrate (10), the substrate (10) being provided with a hollow chamber, a chip assembly (20) being installed in the chamber, and a sample inlet tube (11) and a sample outlet tube (12) being connected to the chip assembly (20) being provided at the front and rear sections of the substrate (10), respectively, wherein: The chip assembly (20) comprises a body (22), a culture chamber (23) is provided in the middle of the body (22), at least two sample introduction substrates (21) are provided at one end of the body (22), and at least two sample output substrates (24) are provided at the other end of the body (22).

2. The intelligent microfluidic living cell culture observation and analysis equipment according to claim 1, characterized in that: The base body (10) is provided with a first through hole (14) which is respectively connected to the sample inlet tube (11) and the sample outlet tube (12), and the first through hole (14) is connected to the chamber.

3. The intelligent microfluidic living cell culture observation and analysis equipment according to claim 2, characterized in that: The sample injection substrate (21) is provided with a first flow groove, a first through hole (14) connected in the direction of the sample injection tube (11) is connected to the first flow groove, and the first flow groove is connected to the culture chamber (23).

4. The intelligent microfluidic living cell culture observation and analysis equipment according to claim 2, characterized in that: The sample outlet substrate (24) is provided with a second flow groove, the first through hole (14) connected in the direction of the sample outlet tube (12) is connected to the second flow groove, and the second through groove is connected to the culture chamber (23).

5. The intelligent microfluidic living cell culture observation and analysis equipment according to claim 1, characterized in that: The culture chamber (23) is provided with first flow channels (60) on both sides, the body (22) has a communication channel connecting the first flow channel (60) and the culture chamber (23), flow blocking columns (50) are arranged in arrays in the communication channel, and arc-shaped flow blocking plates (51) are arranged around the sides of the flow blocking columns (50). The flow blocking plates (51) on adjacent flow blocking columns (50) are spaced apart or abutted.

6. The intelligent microfluidic living cell culture observation and analysis equipment according to claim 5, characterized in that: A thin film is arranged in the communication channel between the first flow channel (60) and the culture chamber (23). The thin film is made of PDMS elastic film material.

7. The intelligent microfluidic living cell culture observation and analysis equipment according to claim 5, characterized in that: The first flow channel (60) has a first inlet (61) at one end and a first outlet (62) at the other end.

8. The intelligent microfluidic living cell culture observation and analysis equipment according to claim 1, characterized in that: An air outlet head (70) is arranged in the hollow chamber of the base body (10), the air outlet head (70) having an air outlet hole (71), the air outlet head (70) being a frustum-shaped structure, the air outlet hole (71) being arranged at the center of the air outlet head (70), a guide surface (72) being arranged around the outside of the air outlet head (70), the guide surface (72) having an inclined angle with the frustum surface of the air outlet head (70).

9. The intelligent microfluidic living cell culture observation and analysis equipment according to claim 1, characterized in that: The bottom of the base (10) is provided with supporting columns (13) in an array.

10. The intelligent microfluidic living cell culture observation and analysis equipment according to claim 1, characterized in that: A cover plate (30) is provided above the hollow chamber of the base body (10).

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