Organic fertilizer for improving yield of psidium guajava planted in saline-alkali soil and preparation method thereof

Abstract

The invention discloses an organic fertilizer for improving yield of guava planted in saline-alkali soil and a preparation method thereof, and relates to the technical field of biology. The active ingredients of the organic fertilizer comprise bacillus horikoshii (ACCC) 01061, bacillus saferae (CICC) 21743, Sporidiobolus salmoninensis (CICC) 32895, Trichoderma koningii (CICC) 13006 and Trichoderma longibrachiatum (ACCC) 32095, and the active ingredients of the organic fertilizer comprise bacillus horikoshii (ACCC) 01061, bacillus saferae (CICC) 21743, Sporidiobolus salmoninensis (CICC) 32895, Trichoderma koningii (CICC) 13006 and Trichoderma longibrachiatum (ACCC) 32095. The organic fertilizer can improve the single-plant yield and fruit quality of guavas planted in saline-alkali soil, and has important market application value.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, in particular to an organic fertilizer for improving the yield of guava planted in saline-alkali land and a preparation method thereof. Background Art

[0002] Guava (scientific name: Psidium guajava L.), also known as guava, chicken shit fruit, and trumpet guava, is a species of tree in the Myrtaceae family. Native to tropical America, guava has since become widely distributed throughout tropical and subtropical regions around the world, having been introduced to my country around the late 17th century. Guava fruit is highly nutritious and is one of the best fruits for beauty and weight loss. The fruit can be eaten fresh or processed into a variety of products, including juice and powder. Guava also has medicinal value, as well as ornamental and landscaping properties.

[0003] The relatively low organic matter content of saline-alkali soils inhibits the process of grassification and the accumulation of organic matter, resulting in poor soil nutrient conditions. The high pH and calcium carbonate content in saline-alkali soils cause phosphorus in the soil to form insoluble phosphates, reducing its availability. Furthermore, saline-alkali soils have poor physical properties, including poor moisture retention and permeability, which hinder the growth of crops. Growing guava in saline-alkali soils can easily lead to flower and fruit drop, resulting in fewer fruit, lower yields, and reduced fruit quality, resulting in significant economic losses.

[0004] The present invention intends to develop an organic fertilizer suitable for application in saline-alkali land to improve the yield and fruit quality of guava. Summary of the Invention

[0005] The present invention aims to provide an organic fertilizer for increasing the yield of guava grown on saline-alkali land and a preparation method thereof, thereby overcoming the problems of the prior art. The organic fertilizer can improve the yield per plant and the quality of guava grown on saline-alkali land, and has important market application value.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] The invention provides a microbial combination for improving the yield of guava planted in saline-alkali land. The microbial combination comprises Bacillus horikoshii ACCC 01061, Bacillus safensis CICC 21743, Sporidiobolus salmonicolor CICC 32895, Trichoderma koningii CICC 13006 and Trichoderma longibrachiatum ACCC 32095.

[0008] The present invention also provides use of the above-mentioned microbial combination in preparing a microbial agent, wherein the microbial agent is used to improve the yield and / or fruit quality of guava grown in saline-alkali land.

[0009] The present invention also provides a microbial agent for improving the yield and / or fruit quality of guava grown in saline-alkali land, wherein the active ingredient comprises the above-mentioned microbial combination.

[0010] The present invention also provides the use of the above-mentioned microbial combination or microbial agent in the preparation of an organic fertilizer for improving the yield and / or fruit quality of guava grown in saline-alkali land.

[0011] The present invention also provides an organic fertilizer for improving the yield and / or fruit quality of guava grown in saline-alkali land, wherein the active ingredient includes the above-mentioned microbial agent.

[0012] Furthermore, the organic fertilizer also includes a fertilizer carrier; the fertilizer carrier includes crop straw and attapulgite powder.

[0013] The crop straw can be selected from one or a combination of corn straw, wheat straw, rice straw, sorghum straw, soybean straw and cotton straw.

[0014] Furthermore, the mass ratio of the crop straw to the attapulgite powder is 7:2.

[0015] The present invention also provides a method for preparing the above-mentioned organic fertilizer, comprising the following steps:

[0016] Bacillus Horikoshii ACCC 01061, Bacillus saffron CICC 21743, Trichoderma salmoniformis CICC 32895, Trichoderma koningii CICC 13006, and Trichoderma longibrachiatus ACCC 32095 were fermented and cultured respectively to obtain fermentation broth of Bacillus Horikoshii ACCC 01061, fermentation broth of Bacillus saffron CICC 21743, fermentation broth of Trichoderma salmoniformis CICC 32895, fermentation broth of Trichoderma koningii CICC 13006, and fermentation broth of Trichoderma longibrachiatus ACCC 32095;

[0017] The fermentation broth of Bacillus Horikoshii ACCC 01061, the fermentation broth of Bacillus sabdariffa CICC 21743, the fermentation broth of Saccharomyces cerevisiae CICC 32895, the fermentation broth of Trichoderma koningii CICC 13006 and the fermentation broth of Trichoderma longibrachiatum ACCC 32095 were concentrated to a viable bacterial content of 10 9 CFU / mL, and then mixed evenly according to the ratio of viable bacteria count of 1:2:1:1:1 to obtain a mixed bacterial solution;

[0018] The crop straw and attapulgite powder were uniformly mixed in a mass ratio of 7:2 to obtain a fertilizer carrier;

[0019] The fertilizer carrier and the mixed bacterial liquid are evenly mixed in a mass ratio of 9:1, and granulated by extrusion to obtain the organic fertilizer.

[0020] The present invention also provides the use of the above-mentioned microbial combination, microbial agent or organic fertilizer in improving the yield and / or fruit quality of guava grown in saline-alkali land.

[0021] The present invention also provides a method for improving the yield and / or fruit quality of guava grown in saline-alkali land, comprising the step of applying the above-mentioned microbial combination, microbial agent or organic fertilizer to the guava tree.

[0022] The present invention discloses the following technical effects:

[0023] The present invention develops an organic fertilizer for increasing the yield of guava grown in saline-alkali soil. The fertilizer is prepared using five active microbial strains: Bacillus horikoshii ACCC 01061, Bacillus saffron CICC 21743, Trichoderma salmoniformis CICC 32895, Trichoderma koningii CICC 13006, and Trichoderma longibrachiatum ACCC 32095. These five microbial strains exhibit a unique synergistic effect. Their combined action optimizes the soil microbial community structure, promotes organic acid production, and enhances soil enzyme activity, thereby increasing the yield per plant and fruit quality of guava grown in saline-alkali soil. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0025] Figure 1 Statistical graph of average yield per plant in each treatment group;

[0026] Figure 2 Statistical graph of VC content in each treatment group;

[0027] Figure 3 Statistical graph of soluble sugar content in each treatment group;

[0028] Figure 4 Statistical graph of solid content in each treatment group. DETAILED DESCRIPTION

[0029] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0030] It should be understood that the terms described herein are intended only to describe particular embodiments and are not intended to limit the present invention. In addition, for numerical ranges herein, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. The intermediate value within any stated value or stated range, and each smaller range between any other stated value or intermediate value within the stated range, is also encompassed within the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded within the scope.

[0031] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of any conflict with any incorporated document, the contents of this specification shall prevail.

[0032] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from the description of the invention. The description and examples are intended to be exemplary only.

[0033] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0034] Yeast extract powder, also known as powdered yeast extract, is made from high-protein baker's or brewer's yeast through autolysis, enzymatic hydrolysis, concentration, and drying. It is a biological culture medium rich in nutrients such as protein, amino acids, peptides, polypeptides, nucleic acids, vitamins, and trace elements. Yeast extract is nutrient-rich and widely used in microbial culture. Yeast extracts used in microbial culture have been referred to as yeast extract (paste) and yeast extract powder (powder). Yeast extract and yeast extract are broadly the same product. Yeast extracts used specifically as fermentation media are generally referred to as yeast extract, while those used as food seasoning ingredients are generally referred to as yeast extract.

[0035] The 2,6-dichloroindophenol titration method is a commonly used method for determining vitamin C (VC) content. This method is based on the reducing properties of vitamin C and the oxidizing properties of 2,6-dichloroindophenol. The specific principles are as follows:

[0036] Principle: Vitamin C has strong reducing properties, while 2,6-dichloroindophenol has strong oxidizing properties. In acidic solutions, 2,6-dichloroindophenol is red, and in neutral or alkaline solutions it is blue. When the blue 2,6-dichloroindophenol solution is used to titrate an acidic solution containing ascorbic acid, ascorbic acid will reduce 2,6-dichloroindophenol to a colorless substance. When all the ascorbic acid in the solution is oxidized, adding 2,6-dichloroindophenol will make the solution red, which is the end point of the titration. Based on the amount of 2,6-dichloroindophenol consumed during the titration, the vitamin C content in the sample can be calculated. The experimental steps are as follows:

[0037] Prepare samples: Weigh a certain amount of fruit or vegetable samples, add oxalic acid solution, grind and filter to obtain sample extract.

[0038] Dye calibration: Take a standard ascorbic acid solution and titrate it with 2,6-dichloroindophenol solution until it turns pink. The end point is that the color does not fade within 30 seconds. Calculate the number of milligrams of ascorbic acid per milliliter of dye.

[0039] Titration sample: Take an appropriate amount of sample extract and titrate with 2,6-dichloroindophenol solution until it turns pink. Record the volume of dye consumed and calculate the vitamin C content in the sample based on the calibration results.

[0040] Scope of application: This method is suitable for the determination of vitamin C content in fruits, vegetables and other foods.

[0041] Anthrone colorimetry is a method used to determine the content of sugars. Its basic principle is that sugars react with anthrone reagents in the presence of concentrated sulfuric acid to produce a blue-green substance. The absorbance of the substance is measured by colorimetry to calculate the sugar content. The experimental steps are as follows:

[0042] Reagent preparation:

[0043] Anthrone reagent: Dissolve 0.4 g of anthrone in 100 mL of 88% sulfuric acid.

[0044] Glucose, fructose, and sucrose standard solutions: Accurately weigh a certain amount of dry analytical pure sugar and prepare a solution of appropriate concentration.

[0045] Draw a standard curve:

[0046] Take standard sugar solutions of different concentrations, add anthrone reagent, measure the absorbance after heating, and draw a standard curve.

[0047] Sample determination:

[0048] Take the sample solution to be tested, add anthrone reagent, and measure the absorbance after heating under the same conditions.

[0049] The sugar content of the samples was calculated using the standard curve.

[0050] Applications: Anthrone colorimetry is widely used to determine the soluble sugar content in fruits and vegetables, as well as the total water-soluble sugar content in plant tissues such as cotton. This method is simple to operate and provides accurate results, making it suitable for the quantitative analysis of a variety of sugars.

[0051] The strains and culture media used in the following examples are as follows:

[0052] The components of LB liquid culture medium are as follows: 5 g / L yeast extract, 10 g / L tryptone and 10 g / L sodium chloride, pH 7.2 (15 g / L agar powder is added to the solid culture medium).

[0053] The components of LB solid medium are as follows: yeast extract 5 g / L, tryptone 10 g / L, sodium chloride 10 g / L and agar powder 15 g / L, pH 7.2.

[0054] The components of NA liquid medium are as follows: peptone 10 g / L, beef extract 3 g / L and sodium chloride 5 g / L, pH 7.2.

[0055] The components of NA liquid medium are as follows: peptone 10 g / L, beef extract 3 g / L, sodium chloride 5 g / L and agar powder 15 g / L, pH 7.2.

[0056] The components of PDA liquid medium are as follows: 200 g / L potato and 20 g / L glucose, with a natural pH.

[0057] The components of PDA solid medium are as follows: 200 g / L potato, 20 g / L glucose and 15 g / L agar powder, with a natural pH.

[0058] The components of the bacterial fermentation medium are as follows, by weight percentage: 1.5% glucose, 1% peptone, 0.6% yeast extract powder, 0.2% potassium dihydrogen phosphate, 0.002% ferrous sulfate heptahydrate, 0.002% zinc sulfate heptahydrate, 0.05% manganese sulfate heptahydrate, 0.03% magnesium sulfate heptahydrate, and the balance water, pH 7.2.

[0059] The components of the yeast fermentation medium are as follows, by weight percentage: 12.0% glucose, 2.0% brown sugar, 3.0% peptone, 1.0% yeast powder, 0.1% potassium dihydrogen phosphate, 1.0% dipotassium hydrogen phosphate, 0.1% magnesium sulfate heptahydrate, 0.1% sodium chloride and the balance water, pH 6.0.

[0060] The components of the mold fermentation medium are as follows, by weight percentage: 3% soluble starch, 1% peptone, 1.5% soybean cake powder, 0.2% dipotassium hydrogen phosphate, 0.05% potassium dihydrogen phosphate, 0.05% magnesium sulfate and the balance water, with a pH of 6.5.

[0061] Bacillus horikoshii ACCC 01061 was purchased from the China Agricultural Culture Collection Center (ACCC) with the strain number ACCC 01061.

[0062] Bacillus safensis CICC 21743 was purchased from China Industrial Culture Collection Center, with the strain number CICC 21743.

[0063] Bacillus saffron CICC 25233 was purchased from China Industrial Microorganism Culture Collection Center, with the strain number CICC25233;

[0064] Sporidiobolus salmonicolor CICC 32895 was purchased from China Industrial Microorganism Culture Collection, with the strain number CICC 32895.

[0065] Trichoderma koningii CICC 13006 was purchased from China Industrial Microorganism Culture Collection, with the strain number CICC 13006;

[0066] Trichoderma longibrachiatum CICC 41491 was purchased from China Industrial Microorganism Culture Collection, with the strain number CICC 41491;

[0067] Trichoderma longibrachiatum ACCC 32095 was purchased from China Agricultural Microbiological Culture Collection Center with the strain number ACCC32095.

[0068] Example 1

[0069] 1. The preparation method of the fermentation broth of Bacillus Horikoshi ACCC 01061 is as follows:

[0070] (1) Bacillus Horikoshii ACCC 01061 was inoculated into LB liquid culture medium and cultured at 37°C with shaking at 180 rpm for 16 h to obtain a bacterial solution of Bacillus Horikoshii ACCC 01061;

[0071] (2) streaking the Bacillus horikoshii ACCC 01061 bacterial solution obtained in step (1) onto an LB solid culture medium plate and culturing in a 37° C. incubator for 48 h;

[0072] (3) Pick a single colony from the plate in step (2) and inoculate it into a flask filled with LB liquid medium and culture it at 37°C with shaking at 180 rpm for 16 h to obtain a seed solution;

[0073] (4) The seed liquid obtained in step (3) was transferred to a fermentation tank loaded with bacterial fermentation medium at a 5% inoculum volume, and cultured continuously for 24 h at 37°C, 200 r / min, and a tank pressure of 0.05 MPa to obtain a fermentation liquid of Bacillus Horikoshi ACCC 01061, which was concentrated to a viable bacterial content of 10 9 CFU / mL, reserve for future use.

[0074] 2. The preparation method of the fermentation broth of Bacillus subtilis CICC 21743 is as follows:

[0075] (1) Bacillus subtilis CICC 21743 was inoculated into LB liquid culture medium and cultured at 37°C with shaking at 180 rpm for 16 h to obtain a bacterial solution of Bacillus subtilis CICC 21743;

[0076] (2) streaking the Bacillus subtilis CICC 21743 culture obtained in step (1) onto an LB solid culture medium plate and culturing in a 37° C. incubator for 48 h;

[0077] (3) Pick a single colony from the plate in step (2) and inoculate it into a flask filled with LB liquid medium and culture it at 37°C with shaking at 180 rpm for 16 h to obtain a seed solution;

[0078] (4) The seed liquid obtained in step (3) was transferred to a fermentation tank loaded with bacterial fermentation medium at an inoculum volume of 5%, and cultured continuously for 24 hours at 37°C, 200 r / min, and a tank pressure of 0.05 MPa to obtain a fermentation liquid of Bacillus subtilis CICC 21743, which was concentrated to a viable bacterial content of 10 9 CFU / mL, reserve for future use.

[0079] 3. The preparation method of the fermentation broth of Saccharomyces cerevisiae CICC 32895 is as follows:

[0080] (1) Inoculate salmon-colored sclerotinia CICC 32895 into NA liquid culture medium and culture with shaking at 30°C and 150 rpm for 24 h to obtain salmon-colored sclerotinia CICC 32895 bacterial liquid;

[0081] (2) streaking the bacterial suspension of the salmon-colored sclerotium CICC 32895 obtained in step (1) onto a NA solid culture medium plate and culturing in a 30° C. incubator for 48 h;

[0082] (3) Pick a single colony from the plate in step (2) and inoculate it into a flask containing NA liquid culture medium and shake culture it at 30°C and 180 rpm for 24 h to obtain a seed solution;

[0083] (4) The seed liquid obtained in step (3) was transferred to a fermentation tank loaded with yeast fermentation medium at an inoculum rate of 5%, and cultured continuously for 24 hours at 30°C, 180 r / min, and a tank pressure of 0.05 MPa to obtain the fermentation liquid of salmon-colored lock yeast CICC 32895, which was concentrated to a viable bacterial content of 10 9 CFU / mL, reserve for future use.

[0084] 4. The fermentation broth of Trichoderma koningii CICC 13006 was prepared as follows:

[0085] (1) Pick a Trichoderma koningii CICC 13006 block and inoculate it into PDA liquid culture medium, shake and culture it at 28°C and 180 rpm for 72 h to obtain a culture solution;

[0086] (2) diluting the culture solution obtained in step (1) and applying it to a PDA solid culture medium, and culturing in a 28°C incubator for 5 days;

[0087] (3) Pick a single colony from the plate in step (2) and inoculate it into a flask containing PDA liquid culture medium, and culture it at 28°C and 200 rpm for 48 hours to obtain seed liquid;

[0088] (4) The seed liquid obtained in step (3) was transferred to a mold fermentation medium, and cultured continuously for 48 hours at 28°C, 220 r / min, and a tank pressure of 0.05 MPa to obtain a fermentation liquid of Trichoderma koningii CICC 13006, which was concentrated to an active spore content of 10 9 CFU / mL, reserve for future use.

[0089] 5. The preparation method of the fermentation broth of Trichoderma longibrachiatum ACCC 32095 is as follows:

[0090] (1) Pick a Trichoderma longibrachiatum ACCC 32095 block and inoculate it into PDA liquid culture medium, shake and culture it at 28°C and 180 rpm for 72 h to obtain a culture solution;

[0091] (2) diluting the culture solution obtained in step (1) and applying it to a PDA solid culture medium, and culturing in a 28°C incubator for 5 days;

[0092] (3) Pick a single colony from the plate in step (2) and inoculate it into a flask containing PDA liquid culture medium, and culture it at 28°C and 200 rpm for 48 hours to obtain seed liquid;

[0093] (4) The seed liquid obtained in step (3) was transferred to the mold fermentation medium, and the culture was continued for 48 hours at 28°C, 220r / min, and a tank pressure of 0.05Mpa to obtain the fermentation liquid of Trichoderma longibrachiatum ACCC 32095, which was concentrated to an active spore content of 10 9 CFU / mL, reserve for future use.

[0094] 6. Preparation of organic fertilizer

[0095] Crop straw and attapulgite powder are used as raw materials to prepare a carrier, which is then evenly mixed with a microbial agent and granulated to produce an organic fertilizer. The crop straw can be selected from one or a combination of corn straw, wheat straw, rice straw, sorghum straw, soybean straw, and cotton straw. The following describes the preparation process of organic fertilizer using rice straw as an example:

[0096] (1) The fermentation broths of Bacillus horikoshii ACCC 01061, Bacillus saffron CICC 21743, Trichoderma salmoninarum CICC 32895, Trichoderma koningii CICC 13006, and Trichoderma longibrachiatum ACCC 32095 were mixed uniformly according to the ratio of the number of viable bacteria (or spores) of 1:2:1:1:1 to obtain a mixed bacterial solution.

[0097] (2) Crush the rice straw and pass it through a 40-mesh sieve to obtain rice straw powder. Mix the rice straw powder and attapulgite powder in a mass ratio of 7:2 to obtain a fertilizer carrier.

[0098] (3) The fertilizer carrier and the mixed bacterial solution are evenly mixed in a mass ratio of 9:1, and granulated by conventional extrusion to obtain granular organic fertilizer.

[0099] Comparative Example 1

[0100] Same as Example 1, except that Bacillus sp. CICC 21743 was replaced by Bacillus sp. CICC 25233.

[0101] Comparative Example 2

[0102] Same as Example 1, except that Trichoderma longibrachiatum ACCC 32095 was replaced by Trichoderma longibrachiatum CICC 41491.

[0103] Comparative Example 3

[0104] The same as Example 1, except that Bacillus Horikoshii ACCC 01061 was removed when preparing the mixed bacterial solution.

[0105] Comparative Example 4

[0106] The same as Example 1, except that Bacillus sabdariffa CICC 21743 was removed when preparing the mixed bacterial solution.

[0107] Comparative Example 5

[0108] The same as Example 1, except that the salmon-colored sclerotinia CICC 32895 was removed when preparing the mixed bacterial solution.

[0109] Comparative Example 6

[0110] The same as Example 1, except that Trichoderma koningii CICC 13006 was removed when preparing the mixed bacterial solution.

[0111] Comparative Example 7

[0112] The same as Example 1, except that Trichoderma longibrachiata ACCC 32095 was removed when preparing the mixed bacterial solution.

[0113] Comparative Example 8

[0114] The same as Example 1, except that the fermentation broth of each strain was replaced by an equal amount of fermentation medium.

[0115] Effect verification example

[0116] 1. Test materials

[0117] The experimental field is located in the experimental demonstration base of Guangdong Academy of Agricultural Sciences. The soil texture is saline-alkali land with a salt content of 0.5% and an alkalinity of 18%.

[0118] Test material: 5-year-old pearl guava.

[0119] Compound fertilizer: 15-15-15 nitrogen, phosphorus and potassium compound fertilizer.

[0120] 2. Experimental Design

[0121] Nine treatment groups were set up, each containing 20 guava trees. Fertilization was applied to the guava trees in each treatment group in February and May. The type and amount of fertilizer applied to each tree are shown in Table 1.

[0122] Table 1 Fertilizer types and fertilizer amounts for different treatment groups

[0123]

[0124]

[0125] 3.Indicator detection

[0126] Multiple batches of mature fruit were harvested and weighed for each treatment group, and the yield per plant was calculated. At maturity, 20 fruits were randomly collected from each treatment group for determination of vitamin C (VC), soluble sugar, and solids content. VC was determined by titration with 2,6-dichloroindophenol; soluble sugar was determined by anthrone colorimetry; and solids were determined by refractometry.

[0127] 4. Test results

[0128] The average yield per plant and the content of VC, soluble sugar and solids in each treatment group are shown in Figures 1-4 . The results show that, on the basis of applying compound fertilizer, the additional application of the organic fertilizer prepared by the present invention can effectively increase the single-plant yield of guava. Moreover, through strain replacement, it was found that the Bacillus sabdariffa and Trichoderma longibrachiatum used in the present invention have certain particularities, and the use of other strains of Bacillus sabdariffa or Trichoderma longibrachiatum did not achieve the expected results. The present invention speculates that there is a special synergistic effect between the Bacillus horikoshii ACCC 01061, Bacillus sabdariffa CICC 21743, Salmonella solani CICC 32895, Trichoderma koningii CICC 13006 and Trichoderma longibrachiatum ACCC 32095 used in the present invention. Under their joint action, the effects of optimizing the soil microbial community structure, promoting the production of organic acids by microorganisms, and enhancing the activity of soil enzymes can be achieved, thereby improving the single-plant yield and fruit quality of guava planted in saline-alkali land.

[0129] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. A microbial combination for increasing the yield of guava grown in saline-alkali land, characterized in that: These include Bacillus horikoshii ACCC 01061, Bacillus safensis CICC 21743, Sporidiobolus salmonicolor CICC 32895, Trichoderma koningii CICC 13006, and Trichoderma longibrachiatum ACCC 32095.

2. Use of the microbial combination according to claim 1 in the preparation of a microbial agent, characterized in that: The microbial agent is used for improving the yield and / or fruit quality of guava grown in saline-alkali land.

3. A microbial agent for improving the yield and / or fruit quality of guava grown in saline-alkali land, characterized in that: The active ingredient comprises the microbial combination according to claim 1.

4. Use of the microbial combination according to claim 1 or the microbial agent according to claim 3 in the preparation of an organic fertilizer for improving the yield and / or fruit quality of guava grown in saline-alkali land.

5. An organic fertilizer for improving the yield and / or fruit quality of guava grown in saline-alkali land, characterized in that: The active ingredient comprises the microbial agent according to claim 3.

6. The organic fertilizer according to claim 5, characterized in that The organic fertilizer also includes a fertilizer carrier; the fertilizer carrier includes crop straw and attapulgite powder.

7. The organic fertilizer according to claim 6, characterized in that The mass ratio of the crop straw to the attapulgite powder is 7:

2.

8. A method for preparing an organic fertilizer as claimed in claim 5, characterized in that: The following steps are involved: Bacillus Horikoshii ACCC 01061, Bacillus saffron CICC 21743, Salmonella saffron CICC 32895, Trichoderma koningii CICC 13006 and Trichoderma longibrachiatum ACCC 32095 are fermented and cultured respectively to obtain Bacillus Horikoshii ACCC 01061 fermentation liquid, Bacillus saffron CICC 21743 fermentation liquid, Salmonella saffron CICC 32895 fermentation liquid, Trichoderma koningii CICC 13006 fermentation liquid and Trichoderma longibrachiatum ACCC 32095 fermentation liquid; The fermentation broth of Bacillus horikoshii ACCC 01061, the fermentation broth of Bacillus sabdariffa CICC 21743, the fermentation broth of Saccharomyces cerevisiae CICC 32895, the fermentation broth of Trichoderma koningii CICC 13006 and the fermentation broth of Trichoderma longibrachiatum ACCC32095 were concentrated to a viable bacterial content of 10 9 CFU / mL, and then mixed evenly according to the ratio of viable bacteria count of 1:2:1:1:1 to obtain a mixed bacterial solution; The crop straw and attapulgite powder are uniformly mixed in a mass ratio of 7:2 to obtain a fertilizer carrier; The fertilizer carrier and the mixed bacterial liquid are uniformly mixed in a mass ratio of 9:1, and the organic fertilizer is obtained by extrusion granulation.

9. Use of the microbial combination according to claim 1, the microbial agent according to claim 3, or the organic fertilizer according to any one of claims 5 to 7 in improving the yield and / or fruit quality of guava grown in saline-alkali land.

10. A method for improving the yield and / or fruit quality of guava grown in saline-alkali land, characterized in that: The method comprises the steps of applying the microbial combination according to claim 1, the microbial agent according to claim 3 or the organic fertilizer according to any one of claims 5 to 7 to the guava tree.

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