Method and apparatus for testing the reuse of centrifuge tubes in ultra-high speed centrifuges

Abstract

This invention provides a method and apparatus for detecting the reuse of centrifuge tubes in ultra-high-speed centrifuges, comprising the following steps: S1, pressurizing and filling a medium into a new centrifuge tube, and detecting the diameter deformation of the centrifuge tube online; S2, using the maximum pressure that the centrifuge tube can withstand to return to its initial diameter as the yield limit pressure value; S3, replacing the centrifuge tube to be reused, pressurizing and filling the centrifuge tube to be reused with a medium, controlling the reuse pressure value at 75%~95% of the yield limit pressure value; S4, detecting the diameter deformation of the centrifuge tube to be reused online; S5, selecting the centrifuge tube that can return to its initial diameter as the reuse centrifuge tube. This invention can detect centrifuge tubes and determine whether they can be safely reused, significantly reducing experimental costs. It can be used to detect whether replacement centrifuge tubes meet the application requirements of ultra-high-speed centrifuges. It prevents substandard centrifuge tubes from entering the ultra-high-speed centrifuge, causing equipment damage or even sample leakage.

Description

Technical Field

[0001] This invention relates to the field of centrifuge tube technology, and in particular to a method and apparatus for detecting the reuse of centrifuge tubes for ultra-high-speed centrifuges. Background Technology

[0002] In virus isolation operations, ultra-high-speed centrifuges with rotation speeds reaching 100,000 rpm are required. At these maximum speeds, the centrifuge tubes must withstand significant gravitational acceleration. Existing centrifuge tubes for these conditions are expensive and can only be used once, resulting in high experimental costs and considerable waste. Furthermore, at high rotation speeds, centrifuge tubes are prone to fatigue damage and breakage during centrifugation, leading to equipment damage or leakage of the sample. Summary of the Invention

[0003] The technical problem to be solved by the present invention is to provide a method for testing the reuse of centrifuge tubes for ultra-high speed centrifuges, which can confirm whether centrifuge tubes can be safely reused, thereby reducing experimental costs.

[0004] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows:

[0005] A method for detecting the reuse of centrifuge tubes for ultra-high-speed centrifuges includes the following steps:

[0006] S1. Pressurize and fill the new centrifuge tube with the medium, and detect the diameter deformation of the centrifuge tube online;

[0007] S2. The maximum pressure that the centrifuge tube can withstand when it returns to its initial diameter is the yield limit pressure value.

[0008] S3. Replace the centrifuge tube to be reused, pressurize the centrifuge tube and fill it with medium, controlling the reuse pressure value to be 75%~95% of the yield limit pressure value;

[0009] S4. Online detection of diameter deformation of centrifuge tubes intended for reuse;

[0010] S5. Select centrifuge tubes that can be restored to their initial diameter as reuse centrifuge tubes.

[0011] In the preferred embodiment, step S2 includes the following steps:

[0012] S21. Pressurize and fill the new centrifuge tube with the medium until the new centrifuge tube is destroyed;

[0013] S22. Record the pressure value that causes the new centrifuge tube to be destroyed, and denote it as the destruction limit pressure value.

[0014] S23. The value is taken as 75% to 95% of the failure limit pressure value, which is the test yield limit pressure value;

[0015] S24. Based on the yield strength pressure value as the basic test data, increase or decrease the pressure value until the maximum pressure value that the centrifuge tube can withstand when it returns to its initial diameter is found.

[0016] In the preferred embodiment, the medium is air or water.

[0017] Another technical problem to be solved by the present invention is to provide an apparatus for detecting the reuse of centrifuge tubes for ultra-high speed centrifuges, which can be used to accurately detect whether centrifuge tubes can be reused.

[0018] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows:

[0019] An apparatus for detecting the reuse of centrifuge tubes in the above-mentioned ultra-high speed centrifuge includes a plunger, a conduit inside the plunger, and the plunger is used to form a sealing structure with the bottle mouth of the centrifuge tube.

[0020] The conduit is connected to the output port of the pressure medium source;

[0021] A pressure sensor is installed on the conduit;

[0022] It is also equipped with a detection device to detect changes in the diameter of the centrifuge tubes.

[0023] In the preferred embodiment, the plunger is fixed on the base plate, and bolts are also fixed on the base plate. A pressure cap is also provided, and the pressure cap has holes corresponding to the bolts. The bolts pass through the holes and are connected to the nuts. The centrifuge tube is used to be placed between the base plate and the pressure cap. The opening of the centrifuge tube is fitted onto the plunger, and a sealing ring is provided on the outer wall of the plunger.

[0024] In a preferred embodiment, a limiting ring is also fixed around the plunger. The limiting ring is located around the centrifuge tube opening and is used to limit the deformation of the centrifuge tube opening.

[0025] In the preferred embodiment, the structure of the pressure medium source is as follows: the outlet of the pump is connected to the inlet of the pressure regulating valve, the outlet of the pressure regulating valve is connected to the inlet of the control valve, and the outlet of the control valve is connected to the conduit.

[0026] The circulation port of the pressure regulating valve is connected to the pump inlet via a circulation pipe;

[0027] A buffer tank is also provided on the conduit, and a compression airbag is installed inside the buffer tank.

[0028] In a preferred embodiment, the detection device includes an image detection device, a photoelectric array detection device, a rangefinder, a travel detection device, and a perimeter detection device;

[0029] The image detection device is used to capture images of centrifuge tubes and detect changes in the diameter of the centrifuge tubes based on the images;

[0030] The photoelectric array detection device uses a counter-beam array to detect changes in the diameter of centrifuge tubes;

[0031] The rangefinder uses laser or sonar to detect the distance between the transmitter head and the outer wall of the centrifuge tube, and calculates the change in the diameter of the centrifuge tube based on the change in distance;

[0032] The stroke detection device uses a detection head to contact the outer wall of the centrifuge tube and measures the displacement of the detection head mechanically, electromagnetically, or photoelectrically. The diameter change of the centrifuge tube is calculated based on the displacement of the detection head.

[0033] The circumference detection device uses a detection tape wrapped around the outer wall of the centrifuge tube. One end of the detection tape is fixed, and the other end is connected to a fixed displacement sensor to detect changes in the circumference of the centrifuge tube and calculate the change in the diameter of the centrifuge tube based on the changes in circumference.

[0034] In the preferred embodiment, one end of the detection belt is fixedly connected to a bolt via a fixed connector, and the displacement sensor is fixedly connected to the current or another bolt.

[0035] Displacement sensors include capacitive, grating, or wound disk displacement sensors.

[0036] In a preferred embodiment, there are multiple plungers on the base plate, and all of the multiple plungers are connected to the conduit;

[0037] There are also multiple corresponding testing devices, each corresponding to a centrifuge tube.

[0038] The present invention provides a method and apparatus for detecting the reuse of centrifuge tubes in ultra-high-speed centrifuges, which has the following beneficial effects:

[0039] 1. The method and apparatus of the present invention can detect centrifuge tubes and determine whether centrifuge tubes can be safely reused, thereby significantly reducing experimental costs.

[0040] 2. The method of this invention can be used to detect whether replacement centrifuge tubes meet the application requirements of ultracentrifuges. This prevents substandard centrifuge tubes from entering the ultracentrifuge, causing equipment damage or even sample leakage.

[0041] 3. The method of the present invention uses pressure-bearing parameters instead of other parameters for evaluation, which simplifies the testing and evaluation process and makes it easier to implement.

[0042] 4. The device of the present invention evaluates the pressure-bearing capacity of centrifuge tubes by assessing the springback of the deformation of the centrifuge tube diameter, thereby enabling a comprehensive assessment of the strength of the centrifuge tubes and avoiding the use of substandard centrifuge tubes in ultra-high-speed centrifuges in a simple way.

[0043] 5. The device of the present invention controls the experimental pressure by means of pressure circulation and balances the pressure fluctuations with a buffer tank, which can provide output pressure simply and accurately.

[0044] 6. The device of the present invention measures the diameter change of centrifuge tubes using the circumference method. It does not have high requirements for the measurement reference and can accurately determine the circumference change of centrifuge tubes, thereby determining the diameter change and ensuring detection accuracy. Attached Figure Description

[0045] The present invention will be further described below with reference to the accompanying drawings and embodiments:

[0046] Figure 1 This is a flowchart of the detection method of the present invention.

[0047] Figure 2 This is the detection device of the present invention.

[0048] Figure 3 The present invention relates to a multi-centrifuge tube detection device.

[0049] Figure 4 This is a schematic diagram of the capacitive detection device of the present invention.

[0050] Figure 5 This is a schematic diagram of the structure of the grating-type detection device of the present invention.

[0051] Figure 6 This is a schematic diagram of the structure of the winding disc type detection device of the present invention.

[0052] Figure 7 This is a schematic diagram of the pressure medium source of the present invention.

[0053] In the diagram: 1. Pressure cap; 2. Bolt; 3. Centrifuge tube; 4. Plunger; 5. Sealing ring; 6. Conduit; 7. Pressure medium source; 71. Circulation pipe; 72. Pump; 73. Pressure regulating valve; 74. Buffer tank; 75. Compressed air bladder; 8. Pressure sensor; 9. Control valve assembly; 10. Displacement sensor; 10. First electrode layer; 101. Second electrode layer; 102. Damper; 103. Insulating section; 104. Electrode medium section; 105. Fixed connector; 106. Metal detection strip; 107. Grating head; 108. Grating guide rail; 109. Absolute value sensor; 110. Winding disc; 111. Detection strip; 11. Exhaust valve; 12. Base plate; 13. Limiting ring; 14. Detailed Implementation

[0054] Example 1:

[0055] like Figure 1 As shown, a method for detecting the reuse of centrifuge tubes in an ultra-high-speed centrifuge includes the following steps:

[0056] S1. Pressurize and fill the new centrifuge tube 3 with medium, and detect the diameter deformation of the centrifuge tube 3 online;

[0057] In this example, centrifuge tube 3 is made of PVC or PC. In a preferred embodiment, the filling medium is either air or water. Alternatively, water is used as the filling medium for PVC centrifuge tube 3, and compressed air is used for PC centrifuge tube 3. PVC is short for polyvinyl chloride, a thermoplastic plastic, divided into two main categories: flexible PVC and rigid PVC. Rigid PVC is used in this example. PC is short for polycarbonate, an engineering plastic with high strength and toughness.

[0058] S2. The maximum pressure that centrifuge tube 3 can withstand when it returns to its initial diameter is the yield limit pressure value.

[0059] In the preferred embodiment, step S2 includes the following steps:

[0060] S21. Pressurize and fill the new centrifuge tube 3 with medium until the new centrifuge tube 3 is destroyed; the destruction of the centrifuge tube 3 means that the deformation of the centrifuge tube 3 cannot be restored, or that cracks appear in the tube wall of the centrifuge tube 3. If any of the above situations occur, it is determined to be destroyed.

[0061] S22. Record the pressure value that causes the new centrifuge tube 3 to be destroyed, and denote it as the destruction limit pressure value.

[0062] S23. The test yield strength pressure value is determined by taking 75% to 95% of the failure limit pressure value.

[0063] Preferably, different values ​​are used as the test yield strength pressure value for different materials. For PVC material, 80% of the failure limit pressure value is used as the yield strength pressure value, and for PC material, 90% of the failure limit pressure value is used as the yield strength pressure value.

[0064] S24. Based on the yield strength pressure value as the basic test data, increase or decrease the pressure value until the maximum pressure value that the centrifuge tube 3 can withstand when it returns to its initial diameter is found.

[0065] S3. Replace the centrifuge tube 3 to be reused, pressurize the centrifuge tube 3 and fill it with medium. The reuse pressure value is controlled at 75%~95% of the yield limit pressure value. For PVC material, the actual test is conducted at 80% of the yield limit pressure value. For PC material, the test is conducted at 90% of the yield limit pressure value. During the test, the pressure is allowed to fluctuate within 5% of the selected pressure value.

[0066] Preferably, the centrifuge tube 3 made of PVC material uses water as the pressure medium, while the centrifuge tube 3 made of PC material uses air as the pressure medium. This is because the breaking limit pressure value of PC material is close to the yield limit pressure value, while air has a certain buffering effect.

[0067] S4. Detect the diameter deformation of the centrifuge tube 3 to be reused online;

[0068] S5. Select centrifuge tube 3 that can be restored to its initial diameter as the reuse centrifuge tube.

[0069] The above methods are used to determine whether centrifuge tube 3 can be reused. The method of the present invention can also be used to judge the quality of centrifuge tube 3, avoiding equipment damage or sample leakage caused by unqualified centrifuge tube 3.

[0070] Example 2:

[0071] like Figure 2 , 3 As shown, an apparatus for the above-mentioned detection method for reuse of centrifuge tubes in ultra-high speed centrifuges includes a plunger 4, a conduit 6 is provided inside the plunger 4, and the plunger 4 is used to form a sealing structure with the bottle mouth of the centrifuge tube 3.

[0072] Conduit 6 is connected to the output port of pressure medium source 7;

[0073] A pressure sensor 8 is installed on the conduit 6;

[0074] A detection device is also provided to detect changes in the diameter of the centrifuge tube 3. With this structure, during use, a pressure medium is introduced into the centrifuge tube 3 from the pressure medium source 7, and then the diameter change of the centrifuge tube 3 under different pressures is detected. This structure allows for convenient pressure resistance testing of the centrifuge tube 3.

[0075] Preferred solutions include Figure 2 , 3 As shown, the plunger 4 is fixed to the base plate 13, and a bolt 2 is also fixed to the base plate 13. A pressure cap 1 is also provided, with holes corresponding to the bolt 2. The bolt 2 passes through the holes and is connected to a nut. A centrifuge tube 3 is positioned between the base plate 13 and the pressure cap 1, with the opening of the centrifuge tube 3 fitted onto the plunger 4. A sealing ring 5 is provided on the outer wall of the plunger 4. This structure connects the centrifuge tube 3 to the plunger 4 and prevents the centrifuge tube 3 from detaching from the plunger 4.

[0076] Preferred solutions include Figure 3 As shown, a limiting ring 14 is also fixed around the plunger 4. The limiting ring 14 is located around the opening of the centrifuge tube 3 and is used to limit the deformation of the opening of the centrifuge tube 3. This structure prevents the centrifuge tube 3 from being damaged at the opening and also prevents leakage caused by deformation of the opening of the centrifuge tube 3.

[0077] Preferred solutions include Figure 7 As shown, the structure of the pressure medium source 7 is as follows: the outlet of the pump 72 is connected to the inlet of the pressure regulating valve 73, the outlet of the pressure regulating valve 73 is connected to the inlet of the control valve, and the outlet of the control valve is connected to the conduit 6.

[0078] The circulation port of the pressure regulating valve 73 is connected to the inlet of the pump 72 through the circulation pipe 71;

[0079] A buffer tank 74 is also provided on the conduit 6, and a compression bladder 75 is installed inside the buffer tank 74. In this example, the pump 72 is a plunger pump to obtain a higher output pressure, such as above 2 MPa. The pressure regulating valve 73 is a pilot-operated pressure regulating valve, which has a structure that provides high pressure accuracy control. The control valve is an electrically controlled shut-off valve. In a preferred embodiment, the pressure regulating valve 73 is an electrically controlled pressure regulating valve, which can automatically regulate the output pressure, thereby improving the efficiency of the experiment and avoiding losses caused by operational errors.

[0080] The pressure medium source 7 is used by setting the circulating pressure of the pressure regulating valve 73. When the output pressure of the pump 72 is higher than the set pressure, the medium enters the inlet of the pump 72 through the circulation pipe 71. The buffer tank 74 is used to buffer the pressure fluctuations of the plunger pump. In use, the circulating pressure of the pressure regulating valve 73 is first set, so that the pump 72 continuously supplies the pressure medium to the buffer tank 74 at the preset pressure until the pressure reaches the preset value. Then, the pump 72 is turned off, and the pressure is maintained by the one-way valve at the outlet of the pump 72. The pressure of the pressure sensor 8 is checked to see if it has reached the preset value. The set pressure is usually higher than the pressure required for the experiment because some pressure is lost when the control valve is opened. After opening the control valve, the experiment is completed, and the diameter of the centrifuge tube 3 is measured by the detection device. Then, the pressure is released by the exhaust valve 12, and a new centrifuge tube 3 is reinstalled for testing. Preferably, the control valve is an electrically controlled valve to achieve automatic control of the experiment and improve the efficiency of the experiment.

[0081] In a preferred embodiment, the detection device includes an image detection device, a photoelectric array detection device, a rangefinder, a travel detection device, and a perimeter detection device;

[0082] In an optional scheme, the image detection device is used to capture high-definition images of the centrifuge tube 3 and detect the diameter change of the centrifuge tube 3 based on the image pixels. During detection, a high-definition camera, such as a 4K camera, is used to capture the initial image of the middle position of the centrifuge tube 3 and the image after the pressure medium is filled. After calibration, the image pixels are correlated with the diameter data. By identifying the edge of the centrifuge tube 3, the diameter change of the centrifuge tube 3 is obtained based on the pixel data.

[0083] In another alternative scheme, the photoelectric array detection device uses a beam array of opposing beams to detect the diameter change of the centrifuge tube 3; by detecting the centrifuge tube with multiple array beams, when the diameter of the centrifuge tube 3 changes, more beams are blocked, and the position of the beam array is correlated with the diameter data to obtain the diameter change of the centrifuge tube 3.

[0084] In another alternative scheme, a rangefinder uses a laser or sonar to detect the distance between the transmitter head and the outer wall of the centrifuge tube 3, and calculates the diameter change of the centrifuge tube 3 based on the distance change. The transmitter head is fixed in position, and first emits a laser or sonar signal. The distance between the transmitter head and the outer wall of the centrifuge tube 3 is calculated based on the flight time of the reflected light or wave. The diameter change of the centrifuge tube 3 is calculated based on the change in distance between the transmitter head and the outer wall of the centrifuge tube 3 under the initial experimental conditions and after the introduction of the pressurized medium.

[0085] In another alternative scheme, the stroke detection device uses a detection head to contact the outer wall of the centrifuge tube 3 and measures the displacement of the detection head mechanically, electromagnetically, or photoelectrically. The diameter change of the centrifuge tube 3 is calculated based on the displacement of the detection head. Taking a micrometer as an example, the detection end of the micrometer is brought into contact with the outer wall of the centrifuge tube 3, and the position of the micrometer is set to zero. When the diameter of the centrifuge tube 3 changes, the value of the diameter change is obtained from the displacement of the micrometer detection rod.

[0086] For some plastics, such as PC, the breaking pressure limit and yield strength are quite close, thus requiring precise detection of diameter changes. The circumference detection device uses a detection band 11 wound around the outer wall of the centrifuge tube 3. One end of the detection band 11 is fixed, and the other end is connected to a fixed displacement sensor 10 to detect changes in the circumference of the centrifuge tube 3. The diameter change of the centrifuge tube 3 is then calculated based on the circumference change. This structure provides the highest detection accuracy, reaching up to the nanometer level. This ensures accurate detection results, preventing excessive pressure differences that could damage the centrifuge tube 3 or cause defective centrifuge tubes to pass the inspection.

[0087] Preferred solutions include Figure 2 As shown, one end of the detection belt 11 is fixedly connected to the bolt 2 via a fixing connector 106. Preferably, the detection belt 11 is a metal belt, and the fixing connector 106 is a hook with a magnet. The displacement sensor 10 is fixedly connected to the current bolt or another bolt; that is, the displacement sensor 10 can be fixed to the current bolt or another bolt, and the fixing method is preferably a clamp fixing method. Although the winding of the detection belt 11 on the outer wall of the centrifuge tube 3 is spiral, the change in length of the detection belt 11 is linearly related to the change in diameter. Therefore, the change in diameter of the centrifuge tube 3 can be accurately calculated based on the change in length of the detection belt 11.

[0088] Displacement sensor 10 includes Figure 4Capacitive Figure 5 grating or Figure 6 Winded disc displacement sensor.

[0089] like Figure 4 As shown, the capacitive displacement sensor 10 has a structure in which a detection band 11 passes between the first electrode layer 101 and the second electrode layer 102. An insulating section 104 and an electrode dielectric section 105 are provided on the detection band 11, wherein the lengths of the insulating section 104 and the electrode dielectric section 105 are equal, and their lengths are slightly larger than the range of circumferential deformation of the centrifuge tube 3. For example, the ratio of the lengths of the insulating section 104 and the electrode dielectric section 105 to the circumferential deformation of the centrifuge tube 3 is 1.2:1. A damper 103 is also provided to provide damping for the detection band 11. By detecting the capacitance change between the first electrode layer 101 and the second electrode layer 102, the deformation value of the centrifuge tube 3 can be obtained, and then the diameter change of the centrifuge tube 3 can be calculated. The advantage of this structure is that it is very inexpensive and has sufficient detection accuracy.

[0090] like Figure 5 As shown, the structure of the grating displacement sensor 10 is as follows: a grating guide rail 109 is fixedly installed, a grating head 108 is slidably connected to the grating guide rail 109, and the grating head 108 is fixedly connected to the detection belt 11. The deformation of the circumference of the centrifuge tube 3 causes the grating head 108 to shift, thereby obtaining the deformation value of the circumference of the centrifuge tube 3, and then calculating the change in the diameter of the centrifuge tube 3. The structure of grating displacement detection is existing technology. The advantage of this structure is its very high detection accuracy.

[0091] like Figure 6 As shown, the structure of the winding disc displacement sensor is as follows: the winding disc 111 is connected to the absolute value sensor 110 to drive the absolute value sensor 110 to rotate, thereby obtaining the rotation angle data of the winding disc 111. The detection belt 11 is wound on the winding disc 111. Let the position of the winding disc 111 when it is wound on the centrifuge tube 3 be the initial position, i.e., zero position. During the experiment, the diameter deformation of the centrifuge tube 3 causes the detection belt 11 to move, thereby causing the winding disc 111 to rotate. From the rotation angle data of the absolute value sensor 110, the deformation value of the circumference of the centrifuge tube 3 is calculated, and then the diameter change of the centrifuge tube 3 is calculated. The advantage of this structure is that it is a commercially available product, easy to purchase, and suitable for small-batch experiments.

[0092] Example 3:

[0093] Based on Example 2, the preferred solution is as follows: Figure 3 In the middle, there are multiple plungers 4 on the base plate 13, and all of the multiple plungers 4 are connected to the conduit 6;

[0094] There are also multiple corresponding detection devices, each corresponding to one centrifuge tube 3. This structure allows for the simultaneous detection of multiple centrifuge tubes 3.

[0095] In use, centrifuge tubes 3 are installed onto each plunger 4. Pressure medium source 7 supplies pressure medium. Once the preset value is reached, control valve group 9 is opened, i.e., conduit 6 fills each centrifuge tube 3 with pressure medium. The detection device acquires the diameter change value of the centrifuge tube 3. Then, according to the method in Example 1, it is determined whether the centrifuge tube 3 can be reused. After the test is completed, the pressure medium is first discharged from exhaust valve 12, and then the nut is loosened to remove the centrifuge tube 3.

[0096] The above embodiments are merely preferred technical solutions of the present invention and should not be considered as limitations on the present invention. The embodiments and features described in this application can be arbitrarily combined with each other without conflict. The scope of protection of the present invention should be limited to the technical solutions described in the claims, including equivalent substitutions of the technical features described in the claims. That is, equivalent substitutions and improvements within this scope are also within the scope of protection of the present invention.

Claims

1. A method for detecting the reuse of centrifuge tubes in ultra-high-speed centrifuges, characterized in that... Includes the following steps: S1. Pressurize and fill the new centrifuge tube (3) with medium, and detect the diameter deformation of the centrifuge tube (3) online; The medium is air or water; S2. The maximum pressure that the centrifuge tube (3) can withstand when it returns to its initial diameter is the yield limit pressure value. Step S2 includes the following steps: S21. Pressurize and fill the new centrifuge tube (3) with medium until the new centrifuge tube (3) is destroyed; S22. Record the pressure value that causes the new centrifuge tube (3) to be destroyed, and record it as the destruction limit pressure value. S23. The value is taken as 75% to 95% of the failure limit pressure value, which is the test yield limit pressure value; S24. Based on the yield limit pressure value as the test data, increase or decrease the pressure value until the maximum pressure value that the centrifuge tube (3) can withstand when it returns to its initial diameter is found. S3. Replace the centrifuge tube (3) to be reused, pressurize the centrifuge tube (3) and fill it with medium. The reuse pressure value is controlled at 75% to 95% of the yield limit pressure value. S4. Detect the diameter deformation of the centrifuge tube (3) to be reused online; S5. Select centrifuge tubes (3) that can be restored to their initial diameter as reuse centrifuge tubes.

2. An apparatus for detecting the reuse of centrifuge tubes in an ultra-high-speed centrifuge as described in claim 1, characterized in that: Includes a plunger (4), which has a conduit (6) inside. The plunger (4) is used to form a sealing structure with the bottle mouth of the centrifuge tube (3). The plunger (4) is fixed on the base plate (13). A bolt (2) is also fixed on the base plate (13). A pressure cap (1) is also provided. The pressure cap (1) has a hole corresponding to the bolt (2). The bolt (2) passes through the hole and is connected to the nut. The centrifuge tube (3) is used to be set between the base plate (13) and the pressure cap (1). The opening of the centrifuge tube (3) is sleeved on the plunger (4). A sealing ring (5) is provided on the outer wall of the plunger (4). A limiting ring (14) is also fixed around the plunger (4). The limiting ring (14) is located around the opening of the centrifuge tube (3) and is used to limit the deformation of the opening of the centrifuge tube (3). The structure of the pressure medium source (7) is as follows: the outlet of the pump (72) is connected to the inlet of the pressure regulating valve (73), the outlet of the pressure regulating valve (73) is connected to the inlet of the control valve, and the outlet of the control valve is connected to the conduit (6). The circulation port of the pressure regulating valve (73) is connected to the inlet of the pump (72) through the circulation pipe (71); A buffer tank (74) is also provided on the conduit (6), and a compression airbag (75) is provided inside the buffer tank (74). The conduit (6) is connected to the output port of the pressure medium source (7); A pressure sensor (8) is provided on the conduit (6); It is also equipped with a detection device, which is used to detect the diameter change of the centrifuge tube (3); The detection device is a perimeter detection device. The perimeter detection device uses a detection belt (11) to be wound around the outer wall of the centrifuge tube (3). One end of the detection belt (11) is fixed, and the other end is connected to a fixed displacement sensor (10) to detect the perimeter change of the centrifuge tube (3) and calculate the diameter change of the centrifuge tube (3) based on the perimeter change. One end of the detection band (11) is fixedly connected to the bolt (2) via a fixed connector (106), and the displacement sensor (10) is fixedly connected to the current or another bolt; The displacement sensor (10) includes capacitive, grating or spiral disk displacement sensors.

3. The apparatus for detecting the reuse of centrifuge tubes in ultra-high-speed centrifuges according to claim 2, characterized in that: There are multiple plungers (4) on the base plate (13), and all of the multiple plungers (4) are connected to the conduit (6); There are also multiple detection devices, each corresponding to a centrifuge tube (3).

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