Method for determining sucrose content of sugarcane stem

Abstract

The method comprises the following steps: weighing the sugarcane stems, preparing sugarcane juice after high-pressure sterilization, drying and weighing filter residues, clarifying the sugarcane juice, measuring the sucrose content, calculating the weight of the sugarcane juice, finally calculating the sucrose content of the sugarcane stems and the like. According to the method, the accuracy of each step is ensured through accurate weighing of the sugarcane stems, preparation and filtration of the sugarcane juice, drying and weighing of the bagasse and the like, interference of non-sucrose optical rotation substances is effectively removed through the solid clarifying agent, the measurement precision of the sucrose content of the sugarcane juice is improved, and the method is suitable for large-scale popularization and application. Meanwhile, the actual sugar content of the sugarcane stems is directly reflected by calculating the sugar content of the sugarcane stems and the sucrose content of the sugarcane stems, compared with a traditional method, operation steps are simple, time and labor are saved, and meanwhile the sucrose content of the sugarcane can be reflected more accurately.

Description

Technical Field

[0001] The invention relates to the technical field of sucrose content detection, and in particular to a method for determining the sucrose content of a sugarcane stem segment. Background Art

[0002] High sugar breeding is the main goal of sugarcane breeding. Sugarcane sucrose content is the most important indicator to measure the quality of sugarcane / materials, so sugarcane sucrose content testing has become an important part of sugarcane breeding.

[0003] At present, the sugar content detection methods commonly used by sugarcane production and scientific research units in my country are primary polarimetry, secondary polarimetry and cooking method; the analysis of sugarcane juice and bagasse is completed. The GB / T 10499-2014 method directly squeezes the sample through a press to obtain bagasse and juice. Since sucrose has optical activity, the size of the optical rotation value is proportional to the sucrose content. If there are non-sucrose optically active substances in the measured solution, it will have a certain impact on the result. Therefore, the primary polarimetry measures the solution of non-pure sucrose as an approximate value of the sucrose content. Therefore, the results of the primary polarimetry and secondary polarimetry are respectively used to represent the sucrose content and sucrose content of sugarcane. However, the primary polarimetry requires strict control of factors such as sample concentration, solvent selection, measurement temperature and wavelength, which increases the complexity of the operation. The operator needs to have certain professional knowledge and skills; the secondary polarimetry also has the problem that the measurement steps are cumbersome, time-consuming and laborious, resulting in low detection efficiency.

[0004] The cooking method is also time-consuming and energy-consuming. When the cooking time is fixed, temperature control is particularly important. The weight loss of the bagasse solution should be kept within the range of 20-25% of the water loss, so that the sugar in the bagasse can be completely cooked out. Inconsistent control of the entire process by different operators will affect the analysis results. Both methods require a large number of sugarcane samples or special equipment, and are not suitable for testing the sucrose content of a section of sugarcane stem. Summary of the invention

[0005] The purpose of the present invention is to provide a method for determining the sucrose content in a sugarcane stem segment, which is simple to operate, saves trouble and labor, and has high detection efficiency and accuracy, so as to solve the technical problems of the prior art in detecting sugar content, which is cumbersome to operate, time-consuming and labor-intensive, and has low detection efficiency.

[0006] In order to solve the above technical problems, the solution adopted by the present invention is as follows: A method for determining the sucrose content of a sugarcane stem segment comprises the following steps: a. Weighing of sugarcane stem segments: Wipe the cut sugarcane stem segments clean, dry them, weigh them, and record the weight M of the sugarcane stem segments; b. Preparation of sugarcane juice: using a sugarcane guillotine to cut an appropriate amount of small sugarcane segments from each sugarcane stem segment, sterilizing the small sugarcane segments under high pressure to fully separate out the sugar, adding distilled water in a mass ratio of 1:n to the small sugarcane segments, mixing, and putting into a juicer; juicing for about 5 minutes until all the small sugarcane segments are broken, obtaining a diluted mixed sugarcane juice, and filtering to obtain a residue and a filtrate; c. Dry and weigh the residue: squeeze out the water from the residue obtained in step b, put the squeezed water back into the filtrate in step b, and then dry the residue every other day using an electric constant temperature blast drying oven and weigh it. When the difference between the two measurements is less than 0.1 g, record the weight W of the bagasse at this time; d. Determine the sucrose content of sugarcane juice: Take out 100 ml of the filtrate in step c, add 1 g of solid clarifier (one of alkaline lead acetate, lime and alum) in a ratio of 100:1 between the filtrate and the solid clarifier for clarification, quickly shake well, and let stand for 10 minutes; use a pipette to take 1 mL of the upper clear liquid, drop it into the test area of ​​the sucrose meter, measure the sucrose content R of the mixed sugarcane juice, and calculate the sucrose content P of the sugarcane juice; the calculation formula used is as follows:

[0007] Where: P is the sucrose content of sugarcane juice, %; n is the weight ratio of distilled water to sugarcane segments in the mixed sugarcane juice; R is the sucrose content of the mixed sugarcane juice, % e. Measure the weight of sugarcane juice: subtract the weight W of bagasse obtained in step c from the weight M of sugarcane stems obtained in step a to obtain the weight Q of sugarcane juice; the calculation formula used is as follows:

[0008] Where: Q is the weight of sugarcane juice, g; M is the weight of sugarcane stem, g; W is the weight of bagasse, g; f. Calculate the sucrose content of the sugarcane stem segment: The sugar content N of the sugarcane stem segment is calculated by calculating the sucrose content P of the sugarcane juice and the weight Q of the sugarcane juice calculated in steps d and e; the calculation formula used is as follows:

[0009] Where: N is the sugar content of sugarcane stem, g; P is the sucrose content of sugarcane juice, %; After calculating the sugar content N of the sugarcane stem segment, the sucrose content C of the sugarcane stem segment is calculated: the calculation formula used is as follows:

[0010] Where: C is the sucrose content in sugarcane stem segment, %.

[0011] As a further illustration of the present invention, the value of n in step b is a natural number greater than 2.

[0012] As a further explanation of the present invention, the drying frequency in step c is once a day. When the difference between the two measurement results is less than 0.1 g, the weight at this time is taken as the weight W of the bagasse.

[0013] As a further illustration of the present invention, in step b, after the sugarcane stem segments are cut into small sugarcane segments, the small sugarcane segments are sterilized by a high pressure sterilizer, and then the sugarcane juice is prepared.

[0014] As a further illustration of the present invention, the solid clarifier used in step d is one of alkaline lead acetate, lime and alum.

[0015] The beneficial effects of the present invention are as follows: 1. The present invention ensures the accuracy of each step by accurately weighing the sugarcane stem segments, preparing and filtering the sugarcane juice, and drying and weighing the bagasse. The interference of non-sucrose optically active substances is effectively removed by a solid clarifier, thereby improving the determination accuracy of the sucrose content of the sugarcane juice. At the same time, by calculating the sugar content of the sugarcane stem segments and the sucrose content of the sugarcane stem segments, the actual sugar content of the sugarcane stem segments is directly reflected. Compared with the traditional method, the operation steps are simple, time-saving and labor-saving, and the sucrose content of the sugarcane cane can be more accurately reflected.

[0016] 2. The present invention is simple to operate and is suitable for rapid determination of the sucrose content of a section of sugarcane stem in the laboratory without the need for a large number of sugarcane samples or special equipment. This is of great significance for high-sugar breeding screening and quality evaluation in sugarcane breeding. Researchers can easily use this method to quickly test a small amount of sugarcane stem segments, obtain sugar content data in a timely manner, and provide a basis for breeding decisions. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a flow chart of the method for determining the sucrose content of sugarcane stem segments of the present invention; DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] The following is a further detailed description of a method for determining sucrose content in a sugarcane stem segment of the present invention in conjunction with the accompanying drawings: Example

[0020] A method for determining the sucrose content of a sugarcane stem segment comprises the following steps: a. Weighing of sugarcane stem segments: Wipe the cut sugarcane stem segments clean, dry them, weigh them, and record the weight of the sugarcane stem segments M, M=496g; b. Preparation of sugarcane juice: using a sugarcane guillotine to cut an appropriate amount of small sugarcane segments from each sugarcane stem segment, sterilizing the small sugarcane segments by high pressure, adding distilled water with a mass ratio of 1:n to the small sugarcane segments, n=4, and putting them into a juicer; juicing for about 5 minutes until all the small sugarcane segments are broken, obtaining a diluted mixed sugarcane juice, and filtering to obtain a residue and a filtrate; c. Take the residue and dry it and weigh it: squeeze out the water from the residue obtained in step c, put the squeezed water back into the filtrate in step b, and then use an electric constant temperature blast drying oven to dry the residue every other day and weigh it. When the difference between the two measurements is less than 0.1 g, record the weight of the bagasse at this time, W = 148.8g; d. Determine the sucrose content of sugarcane juice: Take out 100 mL of the filtrate in step c, add 1 g of solid clarifier alkaline lead acetate according to the ratio of filtrate to solid clarifier of 100:1 for clarification, shake well quickly, and let stand for 10 min; use a pipette to take 1 ml of the upper clear liquid, drop it into the test area of ​​the sucrose meter, measure the sucrose content R of the mixed sugarcane juice, R=6%, and calculate the sucrose content P of the sugarcane juice; the calculation formula used is as follows: =4*6%=24% Where: P is the sucrose content of sugarcane juice, %; n is the weight ratio of distilled water to sugarcane stem segments in the mixed sugarcane juice; R is the sucrose content of the mixed sugarcane juice, % e. Measure the weight of sugarcane juice: subtract the weight W of bagasse obtained in step c from the weight M of sugarcane stems obtained in step a to obtain the weight Q of sugarcane juice; the calculation formula used is as follows: =496-148.8=347.2 Where: Q is the weight of sugarcane juice, g; M is the weight of sugarcane stem, g; W is the weight of bagasse, g; f. Calculate the sucrose content of the sugarcane stem segment: The sugar content N of the sugarcane stem segment is calculated by calculating the sucrose content P of the sugarcane juice and the weight Q of the sugarcane juice calculated in steps d and e; the calculation formula used is as follows: =24%*347.2=83.328=24%*347.2=83.328 Where: N is the sugar content of sugarcane stem, g; P is the sucrose content of sugarcane juice, %; After calculating the sugar content N of the sugarcane stem segment, the sucrose content C of the sugarcane stem segment is calculated: the calculation formula used is as follows: =(83.328 / 496)*100%=16.8% Where: C is the sucrose content of the sugarcane stem segment, %. Take another sugarcane stem segment and repeat steps a to f, where M=568g, n=6, R=4%, W=181.76g; the calculation results are: P=24%, Q=386.24g, N=92.7g, C=16.3%; After comprehensive calculation, it can be found that the sucrose content of this sugarcane stem segment fluctuates around 16.5%.

[0021] The method for calculating the weight of the sugarcane juice in step e complies with the national standard GB / T 10499-2014. Example

[0022] The difference from Example 1 is that the solid clarifier used is alum, which is mixed with distilled water in a mass ratio of 1:n to the sugarcane segment, where n=5; The weight of the sugarcane stem segment in step a is M = 500 g; In step c, when the error is less than 0.1 g, the weight of the weighed bagasse is W=159.1 g; Step d: The sucrose content of the mixed sugarcane juice measured by the sucrose meter is R=5%, and the sucrose content of the sugarcane juice is calculated; the calculation formula used is as follows: =5*5%=25%=5*5%=25% Where: P is the sucrose content of sugarcane juice, %; n is the weight ratio of distilled water to sugarcane stem segments in the mixed sugarcane juice; R is the sucrose content of the mixed sugarcane juice, % e. Measure the weight of sugarcane juice: subtract the weight W of bagasse obtained in step c from the weight M of sugarcane stems obtained in step a to obtain the weight Q of sugarcane juice; the calculation formula used is as follows: =500-159.1=340.9g=500-159.1=340.9g Where: Q is the weight of sugarcane juice, g; M is the weight of sugarcane stem, g; W is the weight of bagasse, g; f. Calculate the sucrose content of the sugarcane stem segment: The sugar content N of the sugarcane stem segment is calculated by calculating the sucrose content P of the sugarcane juice and the weight Q of the sugarcane juice calculated in steps d and e; the calculation formula used is as follows: =25%*340.9=85.225=25%*340.9=85.225 Where: N is the sugar content of sugarcane stem, g; P is the sucrose content of sugarcane juice, %; After calculating the sugar content N of the sugarcane stem segment, the sucrose content C of the sugarcane stem segment is calculated: the calculation formula used is as follows: =(85.225 / 500)*100%=17.045%=(85.225 / 500)*100%=17.045% Where: C is the sucrose content in sugarcane stem segment, %.

[0023] Take another sugarcane stem segment and repeat steps a to f, where M = 530 g, n = 6, R = 4%, W = 159 g; the calculation results are: P = 24%, Q = 371 g, N = 89.04 g, C = 16.8%; After comprehensive calculation, it can be found that the sucrose content of this sugarcane stem segment fluctuates around 17%.

[0024] The working principle of this embodiment is the same as that of Embodiment 1.

[0025] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for determining the sucrose content of a sugarcane stem segment, characterized in that: The following steps are involved: a. Weighing of sugarcane stem segments: Wipe the cut sugarcane stem segments clean, dry them, weigh them, and record the weight M of the sugarcane stem segments; b. Preparation of sugarcane juice: using a sugarcane guillotine to cut an appropriate amount of small sugarcane segments from each sugarcane stem segment, mixing the small sugarcane segments with distilled water at a mass ratio of 1:n, and putting them into a juicer; juicing for about 5 minutes until all the small sugarcane segments are broken up to obtain a diluted mixed sugarcane juice, and filtering to obtain a residue and a filtrate; c. Dry and weigh the residue: squeeze out the water from the residue obtained in step b, put the squeezed water back into the filtrate in step b, use a dryer to dry the residue, weigh it, and record the weight W of the bagasse; d. Determine the sucrose content of sugarcane juice: Take out the filtrate in step c, add a solid clarifier to clarify, quickly shake well, and let stand for 10 minutes; take 1mL of the upper clear liquid and drop it into the test area of ​​the sucrose meter to measure the sucrose content R of the mixed sugarcane juice, and calculate the sucrose content P of the sugarcane juice. The calculation formula used is as follows: P=n·R Where: P is the sucrose content of sugarcane juice, %; n is the weight ratio of distilled water to sugarcane segments in the mixed sugarcane juice; R is the sucrose content of the mixed sugarcane juice, %; e. Measure the weight of sugarcane juice: subtract the weight W of bagasse obtained in step c from the weight M of sugarcane stems obtained in step a to obtain the weight Q of sugarcane juice; the calculation formula used is as follows: Q=MW Where: Q is the weight of sugarcane juice, g; M is the weight of sugarcane stem, g; W is the weight of bagasse, g; f. Calculate the sucrose content of the sugarcane stem segment: The sugar content N of the sugarcane stem segment is calculated by calculating the sucrose content P of the sugarcane juice and the weight Q of the sugarcane juice calculated in steps d and e; the calculation formula used is as follows: N=P·Q Where: N is the sugar content of sugarcane stem, g; P is the sucrose content of sugarcane juice, %; After calculating the sugar content N of the sugarcane stem segment, the sucrose content C of the sugarcane stem segment is calculated: the calculation formula used is as follows: Where: C is the sucrose content in the sugarcane stem segment, %.

2. The method for determining the sucrose content of a sugarcane stem segment according to claim 1, characterized in that: The value of n in step b is a natural number greater than 2.

3. The method for determining the sucrose content of a sugarcane stem segment according to claim 1, characterized in that: The drying is performed once a day in step c. When the weight between two measurements is less than 0.1 g, the weight at this time is taken as the weight W of the bagasse.

4. The method for determining the sucrose content of a sugarcane stem segment according to claim 1, characterized in that: In the step b, after the sugarcane stem segments are cut into small sugarcane segments, the small sugarcane segments are sterilized by a high pressure sterilizer, and then the sugarcane juice is prepared.

5. The method for determining the sucrose content of a sugarcane stem segment according to claim 1, characterized in that: The solid clarifier used in step d is one of basic lead acetate, lime and alum.

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