Constant-distance liquid nitrogen fumigation and storage integrated device

Abstract

The invention discloses a constant-distance liquid nitrogen fumigation and storage integrated device, and relates to the technical field of scientific research instruments. The two steps of liquid nitrogen fumigation and storage of seminal fluid can be completed in the liquid nitrogen tank, the steps of liquid nitrogen injection for balancing, fumigation height adjustment and the like are not needed, the operation steps are simplified, and the liquid nitrogen fumigation device can be suitable for liquid nitrogen tanks with different calibers. The device comprises an integrated extraction box, a carrying box and a constant-distance fumigation clamping seat, the integrated extraction box comprises a main box body, an extraction plate, a movable top plate and a handle; the main box body is of a cuboid structure with openings in the front upper surface. The extraction plate is slidably connected to an opening in the front surface of the main box body; the movable top plate is detachably connected to the opening of the upper surface of the main box body; the lower end of the handle is connected to the main box body, and the upper end forms a hanging part; the constant-distance fumigation clamping seat is hung on the lifting handle; a mounting cavity is formed in the constant-distance fumigation clamping seat; the object carrying box is located in the mounting cavity; carrying box clamping grooves are formed in the two opposite side faces of the carrying box.

Description

Technical Field

[0001] The present application relates to the technical field of scientific research instruments, and in particular to an integrated device for constant-distance liquid nitrogen fumigation and storage. Background Art

[0002] Cryopreservation is a process of cooling a sample to an extremely low temperature to preserve organelles, cells, tissues or any other biological structure. In the process of building experimental animal models, it is necessary to ensure that germplasm cells are accessible and usable. For male animals, sperm and semen can be used almost indefinitely after proper cryopreservation, which is an effective way to protect the genetic resources of important model animals. Slow freezing is a common cryopreservation method for mammals such as crab-eating macaques and rhesus monkeys in laboratories. The semen is first mixed with the cryopreservation solution. After the semen mixture is pre-cooled, it is placed in a freezing straw and cooled by liquid nitrogen vapor. When the temperature reaches below -30°C, it can be placed in liquid nitrogen for storage. In experiments on some animals, it is necessary to control the speed of slow freezing precooling, that is, to change the distance between the sample interface and the liquid nitrogen surface. Liquid nitrogen fumigation box is a device used for liquid nitrogen vapor cooling in most laboratories today. Each fumigation requires fresh liquid nitrogen to be injected before it can be used. After the liquid nitrogen fumigation is completed, the liquid nitrogen needs to be re-injected into the liquid nitrogen tank. It is inconvenient to operate and has no frozen storage function. The Chinese patent "A Liquid Nitrogen Fumigation Suspension Device" (publication number: CN220174282U) discloses a suspension device that can complete liquid nitrogen fumigation in a liquid nitrogen tank, including a tank mouth clamp and a suspension member, and the suspension member is provided with a space for accommodating a freezing tube and / or a freezing rod. This device is fixed to the tank mouth of the liquid nitrogen tank. Each fumigation needs to ensure the distance between the liquid nitrogen in the tank and the tank mouth, and a special lid that can match the size of the suspension member is required. And the suspension member only has a fumigation function, and does not have a freezing storage function, and requires another storage component for storage. Summary of the invention

[0003] The embodiments of the present application provide an integrated constant-distance liquid nitrogen fumigation and storage device, which can not only complete the two steps of liquid nitrogen fumigation and storage of semen in a liquid nitrogen tank, but also eliminates the need to inject liquid nitrogen for balance and adjust the fumigation height, thereby simplifying the operating steps and being applicable to liquid nitrogen tanks of different calibers.

[0004] To achieve the above-mentioned objectives, an embodiment of the present application provides an integrated constant-distance liquid nitrogen fumigation and storage device, comprising an integrated extraction box, a carrier box and a constant-distance fumigation card holder; the integrated extraction box comprises a main box body, a extraction plate, a movable top plate and a handle; the main box body is a rectangular structure with openings on the front surface and the upper surface; the extraction plate is slidably connected to the opening on the front surface of the main box body; the movable top plate is detachably connected to the opening on the upper surface of the main box body; the lower end of the handle is connected to the main box body, and the upper end forms a hanging portion; the constant-distance fumigation card holder is hung on the handle; an installation cavity is formed inside the constant-distance fumigation card holder; the carrier box is located in the installation cavity; the carrier box is a rectangular structure with a hollow front surface; and carrier box slots are provided on two opposite sides of the carrier box.

[0005] Furthermore, the main box body includes a bottom plate and a left side plate, a right side plate and a rear side plate fixedly connected to the bottom plate; main box body slots are provided on the inner sides of the left side plate and the right side plate; and the extraction plate can slide up and down in the main box body slots.

[0006] Furthermore, the movable top plate and the extraction plate are connected via a snap-fit ​​connection.

[0007] Furthermore, the hanging portion on the upper end of the handle includes a horizontal bending section and a vertical bending section.

[0008] Furthermore, an information card placement area is provided on the front surface of the carrier box.

[0009] Furthermore, the information card placement area is a panel welded on the front surface of the cargo box; a mounting gap is provided on the panel; and the mounting gap is used for placing the information card.

[0010] Furthermore, there are multiple cargo boxes; two cargo box clamping blocks adapted to the cargo box clamping slots are provided on the upper surface of the cargo box.

[0011] Furthermore, a fixing ring is provided on the side of the constant distance fumigation seat.

[0012] Furthermore, the size of the cargo box is compatible with the size of the installation cavity of the constant distance fumigation card seat and the inner cavity of the integrated extraction box.

[0013] Furthermore, the object carrying box is made of cold fluorine plate.

[0014] Compared with the prior art, this application has the following beneficial effects:

[0015] 1. The constant distance fumigation card seat in the embodiment of the present application is hung on the handle of the integrated extraction box. When liquid nitrogen fumigation is performed, the object box is stuck in the constant distance fumigation card seat; when storing items, there is no need to disassemble the constant distance fumigation card seat, only the extraction plate of the integrated extraction box needs to be lifted up, the object box needs to be placed in, and then the integrated extraction box can be closed, thereby achieving the purpose of performing liquid nitrogen fumigation and storage in the same device to obtain frozen semen with stable quality.

[0016] 2. The embodiment of the present application can realize the integration of liquid nitrogen fumigation and liquid nitrogen freezing. There is no need to perform liquid nitrogen fumigation operations outside the liquid nitrogen storage tank, which reduces the operating equipment and simplifies the operation process. The simple equipment composition and operation process can allow one person to complete sperm freezing alone without the need for the accompaniment of a special person, thereby improving the versatility of the equipment.

[0017] 3. The embodiment of the present application can be used in various laboratories with suitable liquid nitrogen tank calibers. The device has higher mobility and stronger practicality. The liquid nitrogen in the liquid nitrogen tank only needs to be ensured to be at a normal capacity, without having to determine a certain capacity or distance from the can mouth, and the operation is simple.

[0018] 4. The embodiment of the present application can accommodate multiple layers of cargo boxes and has a large storage capacity.

[0019] 5. The embodiments of the present application are compatible with various semen freezing carriers such as wheat straws, quartz glass tubes, and cryotubes. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 This is a schematic diagram of the structure of an integrated extraction box in the constant distance liquid nitrogen fumigation and storage integrated device according to an embodiment of the present application;

[0022] Figure 2 This is a cross-sectional view of the main box in the constant distance liquid nitrogen fumigation and storage integrated device according to an embodiment of the present application;

[0023] Figure 3 This is a schematic diagram of the structure of the object-carrying box in the constant-distance liquid nitrogen fumigation and storage integrated device according to an embodiment of the present application;

[0024] Figure 4 A side view of a carrier box in the constant distance liquid nitrogen fumigation and storage integrated device according to an embodiment of the present application;

[0025] Figure 5It is a schematic structural diagram of the constant distance fumigation card seat in the constant distance liquid nitrogen fumigation and storage integrated device according to an embodiment of the present application. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0027] In the description of the present application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0028] In the description of the present application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0029] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, "plurality" means two or more.

[0030] Reference Figures 1 to 5 The embodiment of the present application provides a constant distance liquid nitrogen fumigation and storage integrated device, including an integrated extraction box 1, a carrier box 2 and a constant distance fumigation card seat 3. When performing liquid nitrogen fumigation, the constant distance fumigation card seat 3 is hung on the integrated extraction box 1, and the carrier box 2 is mounted in the constant distance fumigation card seat 3. When storing, the carrier box 2 is directly placed in the integrated extraction box 1.

[0031] Reference Figure 1 and Figure 2The integrated extraction box 1 includes a main box body 11, an extraction plate 12, a movable top plate 13 and a handle 14. The main box body 11 is a rectangular parallelepiped structure with openings on the front surface and the upper surface. Specifically, the main box body 11 includes a bottom plate 111 and a left side plate 112, a right side plate 113 and a rear side plate 114 fixedly connected to the bottom plate 111. The extraction plate 12 and the rear side plate 114 are both rectangular plates with a height of 30 cm and a length of 14 cm. The left side plate 112 and the right side plate 113 are both rectangular plates with a height of 30 cm and a length of 14.5 cm. The bottom plate 111 and the movable top plate 13 are both rectangular plates with a length of 14 cm and a width of 14.5 cm.

[0032] The inner sides of the left side plate 112 and the right side plate 113 are both provided with a main box body slot 15 opening inward, and the extraction plate 12 is slidably connected to the opening of the front surface of the main box body 11, and can be extracted and slid up and down in the main box body slot 15. The movable top plate 13 is connected to the opening of the upper surface of the main box body 11 through the buckle 16. Specifically, the movable top plate 13 is snap-connected with the extraction plate 12 and the rear side plate 114. After opening the buckle 16, the extraction plate 12 can be lifted and placed, and the items in the integrated extraction box 1 can be stored and retrieved.

[0033] The length of the handle 14 is 50 cm. The lower end of the handle 14 is welded to the outer side of the rear side plate 114 of the main box body 11, and the upper end forms a hanging portion, which includes a horizontal bending section and a vertical bending section. The bending portion is fixed in the slot of the liquid nitrogen tank mouth.

[0034] Reference Figure 5 , the height of the constant distance fumigation seat 3 is equal to the sum of the height of the loading cavity and the required distance above the liquid nitrogen, and the specific height is determined by experiments. The constant distance fumigation seat 3 includes four seat support walls 31 connected end to end, and the interior of the seat support wall 31 forms an installation cavity 32. The thickness of the seat support wall 31 is 1.7cm, the relative inner wall and outer wall are parallel to each other, and the distance between the relative inner walls is 14cm. The horizontal plane area surrounded by the inner wall is the same as the horizontal plane area of ​​the loading box. A fixing ring 33 is fixedly connected at the center of the side of the constant distance fumigation seat 3. Before liquid nitrogen fumigation, the fixing ring 33 must be hung on the handle 14 of the integrated extraction box 1. In this way, it can be ensured that the constant distance fumigation seat 3 will not float away during liquid nitrogen fumigation.

[0035] Reference Figure 3 and Figure 4The loading box 2 is a rectangular parallelepiped structure with a hollow front surface. Specifically, the loading box 2 is made of a cold fluorine plate with a hollow front plate and other fully closed surfaces. A loading cavity 21 is formed inside the loading box 2. An information card placement area 22 is provided on the front surface of the loading box 2. The information card placement area 22 is a panel with a card seam welded on the top and bottom plate edges of the hollow surface, and the information card can be inserted into the panel. The loading cavity 21 is a rectangular parallelepiped with a length and width of 14 cm and a height of 1.5 cm. The loading cavity 21 is used to place items that need to be fumigated and stored with liquid nitrogen, and the items are placed through the hollow surface. The loading space of the loading box 2 is large and is compatible with various semen freezing carriers such as wheat straws, quartz glass tubes, and cryotubes.

[0036] The left and right sides of the object box 2 are both provided with an object box slot 23 with an opening facing downward. The object box slot 23 is 0.5 cm wide and 1.4 cm high. Therefore, when the object box 2 is placed in the constant distance fumigation card seat 3, the object box slot 23 can be clamped on the inner side of the card seat support wall 31.

[0037] In order to increase the storage capacity, the number of the cargo boxes 2 is multiple, and two cargo box blocks 24 that are compatible with the cargo box slots 23 are provided on the upper surface of the cargo box 2. The cargo box block 24 is 14 cm long, 0.4 cm wide, and 1.4 cm high. When multiple cargo boxes 2 are stacked, the cargo box slots 23 located at the bottom are engaged with the cargo box slots 23 of the cargo boxes 2 stacked on top, which plays a fixing role when stacked, and also has a guiding role when storing and accessing items in the cargo cavity 21. In addition, the placement space of the embodiment of the present application is moderate, and it can accommodate frozen carriers of different shapes and lengths, and the liquid nitrogen fumigation and liquid nitrogen storage environment have good sealing properties, and the probability of frozen carriers falling is low.

[0038] The usage process of the embodiment of this application is as follows:

[0039] The integrated extraction box 1 is the main part for fumigating and storing semen in liquid nitrogen. In the liquid nitrogen fumigation and storage state, the extraction plate 12 is in an inactive state and the buckle 16 is in a clamped state to ensure the state of the items placed on the loading platform in the box.

[0040] When storing items separately, the buckle 16 can be opened and the extraction plate 12 can be lifted up to operate. The extraction plate 12 is inserted into the main box body slot 15 and can slide up and down along the main box body slot 15. It can also prevent the extraction plate 12 from touching the items in the box body when being extracted and put down, causing unnecessary mistakes. When the movable top plate 13 is opened, the object box 2 can be put into the integrated extraction box 1 through this opening.

[0041] Before liquid nitrogen fumigation, first insert the item information card into the information card placement area 22, then put the items to be fumigated with liquid nitrogen into the loading cavity 21, and then insert the loading box slot 23 into the card support wall 31 of the constant distance fumigation card seat 3, and ensure that the loading cavity 21 is completely immersed in the hollow structure, and then the liquid nitrogen fumigation procedure can be carried out.

[0042] During the liquid nitrogen fumigation process, the fixed ring 33 can prevent the constant distance fumigation holder 3 from being pushed by liquid nitrogen to move too much during fumigation, causing the loading chamber 21 to bump and affect the fumigation result. After the fumigation is completed, lift the integrated extraction box 1, open the buckle 16 and lift the extraction plate 12 upwards, then detach the loading box 2 from the constant distance fumigation holder 3, and slightly lift the opening surface of the loading chamber 21 upwards to prevent items from falling. Place the detached loading box 2 in the integrated extraction box 1 with the information card placement area 22 facing the extraction plate 12. After it is placed stably, put down the extraction plate 12 and close the buckle 16 to complete the fumigation and storage operations.

[0043] The above are only specific implementations of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. A constant distance liquid nitrogen fumigation and storage integrated device, characterized in that: It comprises an integrated extraction box, a carrying box and a constant distance fumigation card seat; the integrated extraction box comprises a main box body, an extraction plate, a movable top plate and a handle; the main box body is a rectangular parallelepiped structure with openings on the front surface and the upper surface; the extraction plate is slidably connected to the opening on the front surface of the main box body; the movable top plate is detachably connected to the opening on the upper surface of the main box body; the lower end of the handle is connected to the main box body, and the upper end forms a hanging part; the constant distance fumigation card seat is hung on the handle; the interior of the constant distance fumigation card seat forms an installation cavity; the carrying box is located in the installation cavity; the carrying box is a rectangular parallelepiped structure with a hollow front surface; The object carrying box is provided with object carrying box slots on two opposite sides.

2. The constant distance liquid nitrogen fumigation and storage integrated device according to claim 1, characterized in that: The main box body comprises a bottom plate and a left side plate, a right side plate and a rear side plate fixedly connected to the bottom plate; the inner sides of the left side plate and the right side plate are both provided with main box body slots; and the extraction plate can slide up and down in the main box body slots.

3. The constant distance liquid nitrogen fumigation and storage integrated device according to claim 1, characterized in that: The movable top plate is connected to the extraction plate via a buckle.

4. The constant distance liquid nitrogen fumigation and storage integrated device according to claim 1, characterized in that: The hanging portion on the upper end of the handle includes a horizontal bending section and a vertical bending section.

5. The constant distance liquid nitrogen fumigation and storage integrated device according to claim 1, characterized in that: An information card placement area is arranged on the front surface of the object carrying box.

6. The constant distance liquid nitrogen fumigation and storage integrated device according to claim 5, characterized in that: The information card placement area is a panel welded on the front surface of the object carrying box; a mounting gap is arranged on the panel; and the mounting gap is used for placing the information card.

7. The constant distance liquid nitrogen fumigation and storage integrated device according to claim 1, characterized in that: There are multiple cargo boxes; Two object carrying box clamping blocks matched with the object carrying box clamping slots are arranged on the upper surface of the object carrying box.

8. The constant distance liquid nitrogen fumigation and storage integrated device according to claim 1, characterized in that: A fixing ring is arranged on the side of the constant distance fumigation seat.

9. The constant distance liquid nitrogen fumigation and storage integrated device according to claim 1, characterized in that: The size of the cargo box is compatible with the size of the installation cavity of the constant distance fumigation card seat and the inner cavity of the integrated extraction box.

10. The constant distance liquid nitrogen fumigation and storage integrated device according to claim 1, characterized in that: The object carrying box is made of cold fluorine plate.

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