Method for extracting total flavonoids from idesia polycarpa cakes

Abstract

The invention relates to the technical field of total flavone extraction. The invention provides an extraction method of idesia cake total flavonoids, which comprises the following steps: (1) crushing and drying idesia cake obtained after pretreatment to obtain idesia cake powder; and (2) mixing the idesia cake meal powder with an organic solvent, performing ultrasonic treatment, separating, performing rotary evaporation, and concentrating to obtain the idesia cake meal. According to the extraction process and setting of parameter conditions, the extraction time can be effectively shortened, the extraction efficiency is improved, and wide application value is provided for development and utilization of idesia cake flavone.

Description

Technical Field

[0001] The invention relates to the technical field of total flavonoids extraction, in particular to a method for extracting total flavonoids from Castanopsis schrenkiana cake. Background Art

[0002] Castanopsis truncatum, also known as oil grape, chair tree and water winter melon, is a plant with ecological and economic functions. It belongs to the deciduous tree of the genus Castanopsis in the family Caryophyllaceae and is an important woody oil tree species in my country. The oil content in Castanopsis truncatum fruit is greater than 20%, with a high oil content. It is rich in unsaturated fatty acids, and the main component is linoleic acid (relative content accounts for about 60%). Castanopsis truncatum fruit contains a variety of biologically active ingredients, such as flavonoids, terpenes, alkaloids, etc., which have anti-inflammatory, antibacterial, antioxidant, anti-tumor and other pharmacological effects, and have the potential for further development and utilization. Due to its good health and medicinal effects, it has been used to produce edible oil in southwest China for many years, and it is also a source of biodiesel fuel.

[0003] The cake of ash tree fruit is a byproduct of oil extraction from ash tree fruit. Its yield is more than 60%, and it is generally abandoned or used as a relatively good feed resource and soil compost, with low utilization rate. It is reported that foods rich in flavonoids can play a protective role in different diseases and have a variety of pharmacological activities, including antioxidant, anti-allergic, anti-inflammatory, anti-diabetic, etc., protecting the liver and stomach, and antiviral, antibacterial and anticancer activities. Flavonoids also have conjugated ring structures and hydroxyl groups, and have the ability to scavenge free radicals and reactive oxygen. It is precisely because plant flavonoids have excellent biocompatibility, drug function and therapeutic activity that the development of total flavonoids in ash tree fruit cake has a very broad development prospect. However, the current research on ash tree fruit mainly focuses on ash tree fruit oil, and the research on its byproduct cake is not thorough enough, and the total flavonoids contained in its cake need further in-depth research. Summary of the invention

[0004] The purpose of the present invention is to provide a method for extracting total flavonoids from Castanopsis schrenkiana cake. The extraction process of the present invention can effectively shorten the extraction time and improve the extraction efficiency, which provides wide application value for the development and utilization of flavonoids from Castanopsis schrenkiana cake.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The present invention provides a method for extracting total flavonoids from tung oil tree seed cake, comprising the following steps:

[0007] (1) crushing and drying the tung oil tree cake obtained after the pretreatment to obtain tung oil tree cake powder;

[0008] (2) mixing the ashwagandha seed cake powder with an organic solvent, performing ultrasonic treatment and separation to obtain a ashwagandha seed cake powder total flavonoids solution;

[0009] (3) The total flavonoids solution of the ash cake powder is subjected to rotary evaporation and concentration to obtain the flavonoids solution.

[0010] Preferably, the castanea seed cake in step (1) is crushed and then sieved, and the mesh size of the sieve is 30-50 meshes.

[0011] Preferably, the drying temperature in step (1) is 50-70°C.

[0012] Preferably, the organic solvent in step (2) comprises ethanol, and the mass concentration of the ethanol is 70-90%; the solid-liquid ratio of the mixture of the Castanopsis schrenkiana cake powder and the organic solvent is 1g: (30-60)mL.

[0013] Preferably, the power of the ultrasound in step (2) is 300-500 W, the temperature of the ultrasound is 50-80° C., and the time of the ultrasound is 50-70 min.

[0014] Preferably, the separation in step (2) is carried out by centrifugation, the centrifugal speed is 7000-8500 r / min, and the centrifugal time is 5-15 min.

[0015] Preferably, step (3) is concentrated to 1 / 20-1 / 5 of the volume of the total flavonoid solution of the Castanopsis schrenkiana cake powder.

[0016] By adopting the above technical solution, the present invention has the following beneficial effects:

[0017] The organic solvent type, organic solvent concentration, ultrasound, centrifugation and other parameter conditions set in the technical scheme of the present invention can better promote the release of bioactive substances in the Castanopsis schrenkiana cake, shorten the extraction time, and effectively improve the purity and extraction rate of total flavonoids; at the same time, the extraction process is stable in operation and low in cost, and can be better applied to the development and utilization of Castanopsis schrenkiana cake flavonoids. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is the standard curve of the reference substance rutin. DETAILED DESCRIPTION

[0019] The present invention provides a method for extracting total flavonoids from tung oil tree seed cake, comprising the following steps:

[0020] (1) crushing and drying the tung oil tree cake obtained after the pretreatment to obtain tung oil tree cake powder;

[0021] (2) mixing the ashwagandha seed cake powder with an organic solvent, performing ultrasonic treatment and separation to obtain a ashwagandha seed cake powder total flavonoids solution;

[0022] (3) The total flavonoids solution of the ash cake powder is subjected to rotary evaporation and concentration to obtain the flavonoids solution.

[0023] In the present invention, fresh Alpinia schrenkiana fruits are cleaned and then dried and subjected to oil extraction to obtain Alpinia schrenkiana cake. The drying temperature of the present invention is preferably 50-70°C, more preferably 55-65°C, and more preferably 60°C; the drying time is preferably 45-55h, more preferably 46-50h, and more preferably 48h; and the oil extraction method is preferably cold pressing.

[0024] In the present invention, the obtained ashwagandha cake is then crushed and dried to obtain ashwagandha cake powder. The ashwagandha cake in the present invention is crushed and passed through a sieve, and the sieve underflow is collected. The mesh number of the sieve is preferably 30-50 mesh, more preferably 35-45 mesh, and more preferably 40 mesh. The collected sieve underflow is then dried, and the drying temperature is preferably 50-70°C, more preferably 55-65°C, and more preferably 60°C; the drying time is preferably 6-24h, more preferably 10-20h, and more preferably 12h.

[0025] In the present invention, the obtained ashwagandha cake powder is mixed with an organic solvent for ultrasonic treatment, and the organic solvent is preferably ethanol, and the mass concentration of the ethanol is preferably 70-90%, further preferably 75-85%, and further preferably 80%. The solid-liquid ratio of the ashwagandha cake powder and the organic solvent in the present invention is preferably 1g: (30-60) mL, further preferably 1g: (40-55) mL, and further preferably 1g: 50 mL. The power of the ultrasound in the present invention is preferably 300-500W, further preferably 350-450W, and further preferably 400W; the temperature of the ultrasound is preferably 50-80°C, further preferably 60-75°C, and further preferably 70°C; the time of the ultrasound is preferably 50-80min, further preferably 60-75min, and further preferably 70min; the number of ultrasounds is preferably 1 time.

[0026] In the present invention, solid-liquid separation is performed after ultrasonic treatment, and the supernatant is collected to obtain a total flavonoid solution of the tung oil tree seed cake powder. The solid-liquid separation method of the present invention is preferably centrifugation, and the centrifugal speed is preferably 7000-8500r / min, further preferably 7500-8200r / min, and further preferably 8000r / min; the centrifugal time is preferably 5-15min, further preferably 8-12min, and further preferably 10min.

[0027] In the present invention, the obtained total flavonoids solution of the tung oilseed cake powder is subjected to rotary evaporation, and the temperature of the rotary evaporation is preferably 50-70° C., further preferably 55-65° C., and further preferably 60° C. After rotary evaporation, the total flavonoids solution of the tung oilseed cake powder is preferably concentrated to 1 / 20-1 / 5 of the volume of the total flavonoids solution, further preferably 3 / 40-5 / 40, and further preferably 1 / 10.

[0028] In the present invention, the concentrated total flavonoids solution of Castanopsis schrenkiana cake powder is preferably subjected to low-temperature freezing, and the freezing temperature is preferably -75°C to -85°C, further preferably -78°C to -82°C, and further preferably -80°C; the freezing time is preferably 24-52h, further preferably 36-50h, and further preferably 48h.

[0029] In the present invention, the crude total flavonoids of Castanopsis schrenkiana are obtained after low-temperature freezing and freeze-drying. The freeze-drying time of the present invention is preferably 12-52 hours, more preferably 24-50 hours, and even more preferably 48 hours.

[0030] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0031] Example 1

[0032] A method for extracting total flavonoids from tung oil seed cake, comprising the following steps:

[0033] (1) Cleaning fresh Alpinia schrenkiana fruits, drying them at 60°C for 48 hours, removing branches and leaves, and extracting oil by cold pressing to obtain Alpinia schrenkiana cake;

[0034] (2) After the ash cake is crushed, it is passed through a 40-mesh sieve, and the sieved material is collected and dried at 60° C. for 12 h to obtain ash cake powder;

[0035] (3) Mix the ash cake powder and 80% ethanol at a ratio of 1 g:50 mL and let stand for 5 min, then ultrasonically treat at 70 ° C and 400 W for 70 min, then centrifuge at 8000 r / min for 10 min to separate the solid and liquid, collect the supernatant, and obtain the ash cake powder total flavonoids solution;

[0036] (4) The total flavonoids solution of Castanopsis schrenkiana cake powder was concentrated to 1 / 10 of the volume of the total flavonoids solution of Castanopsis schrenkiana cake powder by rotary evaporation at 60°C, and then frozen at -80°C. After being taken out, it was placed in a freeze dryer and dried for 48 hours to obtain the crude total flavonoids of Castanopsis schrenkiana.

[0037] Example 2

[0038] A method for extracting total flavonoids from tung oil seed cake, comprising the following steps:

[0039] (1) Cleaning fresh Alpinia schrenkiana fruits, drying them at 60°C for 48 hours, removing branches and leaves, and extracting oil by cold pressing to obtain Alpinia schrenkiana cake;

[0040] (2) After the ash cake is crushed, it is passed through a 40-mesh sieve, and the sieved material is collected and dried at 60° C. for 12 h to obtain ash cake powder;

[0041] (3) Mix the ash cake powder and 80% ethanol at a ratio of 1 g:50 mL and let stand for 5 min, then ultrasonically treat at 70°C and 450 W for 60 min, then centrifuge at 8000 r / min for 10 min to separate the solid and liquid, collect the supernatant, and obtain the ash cake powder total flavonoids solution;

[0042] (4) The total flavonoids solution of Castanopsis schrenkiana cake powder was concentrated to 1 / 10 of the volume of the total flavonoids solution of Castanopsis schrenkiana cake powder by rotary evaporation at 60°C, and then frozen at -80°C. After being taken out, it was placed in a freeze dryer and dried for 48 hours to obtain the crude total flavonoids of Castanopsis schrenkiana.

[0043] Example 3

[0044] A method for extracting total flavonoids from tung oil seed cake, comprising the following steps:

[0045] (1) Cleaning fresh Alpinia schrenkiana fruits, drying them at 60°C for 48 hours, removing branches and leaves, and extracting oil by cold pressing to obtain Alpinia schrenkiana cake;

[0046] (2) After the ash cake is crushed, it is passed through a 40-mesh sieve, and the sieved material is collected and dried at 60° C. for 12 h to obtain ash cake powder;

[0047] (3) Mix the ash cake powder and 80% ethanol at a ratio of 1 g:60 mL and let stand for 5 min, then ultrasonically treat at 70°C and 400 W for 80 min, then centrifuge at 8000 r / min for 10 min to separate the solid and liquid, collect the supernatant, and obtain the ash cake powder total flavonoids solution;

[0048] (4) The total flavonoids solution of Castanopsis schrenkiana cake powder was concentrated to 1 / 10 of the volume of the total flavonoids solution of Castanopsis schrenkiana cake powder by rotary evaporation at 60°C, and then frozen at -80°C. After being taken out, it was placed in a freeze dryer and dried for 48 hours to obtain the crude total flavonoids of Castanopsis schrenkiana.

[0049] Example 4

[0050] A method for extracting total flavonoids from tung oil seed cake, comprising the following steps:

[0051] (1) Cleaning fresh Alpinia schrenkiana fruits, drying them at 60°C for 48 hours, removing branches and leaves, and extracting oil by cold pressing to obtain Alpinia schrenkiana cake;

[0052] (2) After the ash cake is crushed, it is passed through a 40-mesh sieve, and the sieved material is collected and dried at 60° C. for 12 h to obtain ash cake powder;

[0053] (3) Mix the ash cake powder and 80% ethanol at a ratio of 1 g:50 mL and let stand for 5 min, then ultrasonically treat at 70°C and 350 W for 60 min, then centrifuge at 8000 r / min for 10 min to separate the solid and liquid, collect the supernatant, and obtain the ash cake powder total flavonoids solution;

[0054] (4) The total flavonoids solution of Castanopsis schrenkiana cake powder was concentrated to 1 / 10 of the volume of the total flavonoids solution of Castanopsis schrenkiana cake powder by rotary evaporation at 60°C, and then frozen at -80°C. After being taken out, it was placed in a freeze dryer and dried for 48 hours to obtain the crude total flavonoids of Castanopsis schrenkiana.

[0055] Example 5

[0056] A method for extracting total flavonoids from tung oil seed cake, comprising the following steps:

[0057] (1) Cleaning fresh Alpinia schrenkiana fruits, drying them at 60°C for 48 hours, removing branches and leaves, and extracting oil by cold pressing to obtain Alpinia schrenkiana cake;

[0058] (2) After the ash cake is crushed, it is passed through a 40-mesh sieve, and the sieved material is collected and dried at 60° C. for 12 h to obtain ash cake powder;

[0059] (3) Mix the ash cake powder and 80% ethanol at a ratio of 1 g:60 mL and let stand for 5 min, then ultrasonically treat at 70°C and 400 W for 60 min, then centrifuge at 8000 r / min for 10 min to separate the solid and liquid, collect the supernatant, and obtain the ash cake powder total flavonoids solution;

[0060] (4) The total flavonoids solution of Castanopsis schrenkiana cake powder was concentrated to 1 / 10 of the volume of the total flavonoids solution of Castanopsis schrenkiana cake powder by rotary evaporation at 60°C, and then frozen at -80°C. After being taken out, it was placed in a freeze dryer and dried for 48 hours to obtain the crude total flavonoids of Castanopsis schrenkiana.

[0061] Example 6

[0062] A method for extracting total flavonoids from tung oil seed cake, comprising the following steps:

[0063] (1) Cleaning fresh Alpinia schrenkiana fruits, drying them at 60°C for 48 hours, removing branches and leaves, and extracting oil by cold pressing to obtain Alpinia schrenkiana cake;

[0064] (2) After the ash cake is crushed, it is passed through a 40-mesh sieve, and the sieved material is collected and dried at 60° C. for 12 h to obtain ash cake powder;

[0065] (3) Mix the ash cake powder and 80% ethanol at a ratio of 1 g:60 mL and let stand for 5 min, then ultrasonically treat at 70 ° C and 350 W for 70 min, then centrifuge at 8000 r / min for 10 min to separate the solid and liquid, collect the supernatant, and obtain the ash cake powder total flavonoids solution;

[0066] (4) The total flavonoids solution of Castanopsis schrenkiana cake powder was concentrated to 1 / 10 of the volume of the total flavonoids solution of Castanopsis schrenkiana cake powder by rotary evaporation at 60°C, and then frozen at -80°C. After being taken out, it was placed in a freeze dryer and dried for 48 hours to obtain the crude total flavonoids of Castanopsis schrenkiana.

[0067] Test Example 1

[0068] (I) Determination of ethanol concentration

[0069] The fresh ashwagandha fruit is cleaned, then dried at 60°C for 48 hours, and after removing the branches and leaves, the oil is extracted by cold pressing to obtain ashwagandha cake; the ashwagandha cake is crushed and passed through a 40-mesh sieve, and the sieved material is collected and dried at 60°C for 12 hours to obtain ashwagandha cake powder.

[0070] Weigh 3 groups of 1g of tung oilseed cake powder, respectively, and mix with 70%, 80%, and 90% ethanol solutions at a solid-liquid ratio of 1g:40mL, let stand for 5min, then ultrasonically treat for 70min at 70℃ and 400W, then centrifuge at 8000r / min for 10min, separate the solid and liquid, collect the supernatant, and obtain the tung oilseed cake powder total flavonoids solution. Then measure the absorbance of the solution and calculate the yield, the yield results are shown in Table 1.

[0071] The total flavonoids solution of Castanopsis schrenkiana cake powder was concentrated to 1 / 10 of the volume of the total flavonoids solution of Castanopsis schrenkiana cake powder by rotary evaporation at 60°C, and then frozen at -80°C. After being taken out, it was placed in a freeze dryer and dried for 48 hours to obtain the crude total flavonoids of Castanopsis schrenkiana.

[0072] The yield of total flavonoids extracted is determined according to the second method for the determination of total flavonoids in health foods in the "Technical Guidelines for Inspection and Evaluation of Physical, Chemical and Hygienic Indicators of Health Foods" (2020 Edition), namely the NaNO2-Al(NO3)3-NaOH method. The specific principle is that the flavonoid components in the sample can be reduced by sodium nitrite to form a complex with aluminum nitrate, which opens under the alkaline conditions of sodium hydroxide solution to generate 2-hydroxychalcone, making the solution a characteristic orange-red color. The absorbance is determined at a wavelength of 510nm by spectrophotometry, and rutin is used as a reference. The standard curve method is used to calculate the content of total flavonoids in the sample. The standard curve is as follows: Figure 1 As shown, the linear regression equation is y = 0.0019x-0.0021, R 2 =0.9994.

[0073] The total flavonoids yield of Castanopsis schrenkiana cake was calculated as follows:

[0074]

[0075] In the formula: w is the extraction amount of total flavonoids from Castanopsis altissima cake (mg / g), c is the concentration of flavonoid compounds (μg / mL), v is the volume of the total flavonoid sample solution for testing (mL), and m is the mass of Castanopsis altissima cake powder (g).

[0076] Table 1 Total flavonoids extraction rate of Alpinia schrenkiana cake in each ethanol concentration group

[0077] Ethanol concentration / % 70 80 90 Yield / % 3.81 4.58 4.43

[0078] As shown in Table 1, the total flavonoids yield is highest when the ethanol concentration is 80%, so 80% ethanol concentration is selected.

[0079] (II) Determination of ultrasonic power

[0080] The fresh ashwagandha fruit is cleaned, then dried at 60°C for 48 hours, and after removing the branches and leaves, the oil is extracted by cold pressing to obtain ashwagandha cake; the ashwagandha cake is crushed and passed through a 40-mesh sieve, and the sieved material is collected and dried at 60°C for 12 hours to obtain ashwagandha cake powder.

[0081] Weigh 3 groups of 1g of tung oilseed cake powder, respectively, and mix with 80% ethanol solution at a solid-liquid ratio of 1g:40mL, let stand for 5min, then ultrasonically treat at 350W, 400W, and 450W at 70℃ for 70min, then centrifuge at 8000r / min for 10min, separate the solid and liquid, collect the supernatant, and obtain the tung oilseed cake powder total flavonoids solution. Then measure the absorbance of the solution and calculate the yield, the yield results are shown in Table 2.

[0082] The total flavonoids solution of Castanopsis schrenkiana cake powder was concentrated to 1 / 10 of the volume of the total flavonoids solution of Castanopsis schrenkiana cake powder by rotary evaporation at 60°C, and then frozen at -80°C. After being taken out, it was placed in a freeze dryer and dried for 48 hours to obtain the crude total flavonoids of Castanopsis schrenkiana.

[0083] Table 2 Total flavonoids extraction rate of each liquid ratio group

[0084] Ultrasonic power / W 350 400 450 Yield / % 4.31 4.58 4.32

[0085] It can be seen from Table 2 that the total flavonoids yield is highest when the ultrasonic power is 400W, so the ultrasonic power is selected to be 400W.

[0086] 3. Determination of ultrasound time

[0087] The fresh ashwagandha fruit is cleaned, then dried at 60°C for 48 hours, and after removing the branches and leaves, the oil is extracted by cold pressing to obtain ashwagandha cake; the ashwagandha cake is crushed and passed through a 40-mesh sieve, and the sieved material is collected and dried at 60°C for 12 hours to obtain ashwagandha cake powder.

[0088] Weigh 3 groups of 1g of tung oilseed cake powder, respectively, and mix with 80% ethanol solution at a solid-liquid ratio of 1g:40mL, let stand for 5min, then ultrasonically treat at 70℃, 400W for 60min, 70min, 80min, respectively, then centrifuge at 8000r / min for 10min, separate solid and liquid, collect the supernatant, and obtain the tung oilseed cake powder total flavonoids solution. Then measure the absorbance of the solution and calculate the yield, the yield results are shown in Table 3.

[0089] The total flavonoids solution of Castanopsis schrenkiana cake powder was concentrated to 1 / 10 of the volume of the total flavonoids solution of Castanopsis schrenkiana cake powder by rotary evaporation at 60°C, and then frozen at -80°C. After being taken out, it was placed in a freeze dryer and dried for 48 hours to obtain the crude total flavonoids of Castanopsis schrenkiana.

[0090] Table 3 Total flavonoids extraction rate of Castanopsis schrenkiana cake in each ultrasonic time group

[0091] Ultrasonic time / min 60 70 80 Yield / % 4.26 4.58 4.30

[0092] As shown in Table 3, the total flavonoids yield was the highest when the ultrasonic time was 70 min, so the ultrasonic time was selected to be 70 min.

[0093] (IV) Determination of material-liquid ratio

[0094] The fresh ashwagandha fruit is cleaned, then dried at 60°C for 48 hours, and after removing the branches and leaves, the oil is extracted by cold pressing to obtain ashwagandha cake; the ashwagandha cake is crushed and passed through a 40-mesh sieve, and the sieved material is collected and dried at 60°C for 12 hours to obtain ashwagandha cake powder.

[0095] Weigh 3 groups of 1g of tung oilseed cake powder, respectively, and mix with 80% ethanol solution at a solid-liquid ratio of 1g:40mL, 1g:50mL, and 1g:60mL, let stand for 5min, then ultrasonically treat for 70min at 70℃ and 400W, then centrifuge at 8000r / min for 10min, separate the solid and liquid, collect the supernatant, and obtain the tung oilseed cake powder total flavonoids solution. Then measure the absorbance of the solution and calculate the yield, the yield results are shown in Table 4.

[0096] The total flavonoids solution of Castanopsis schrenkiana cake powder was concentrated to 1 / 10 of the volume of the total flavonoids solution of Castanopsis schrenkiana cake powder by rotary evaporation at 60°C, and then frozen at -80°C. After being taken out, it was placed in a freeze dryer and dried for 48 hours to obtain the crude total flavonoids of Castanopsis schrenkiana.

[0097] Table 4 Total flavonoids extraction rate of Alpinia schrenkiana cake in each material-liquid ratio group

[0098] Material-liquid ratio / g:mL 1：40 1：50 1：60 Yield / % 4.58 4.79 4.64

[0099] It can be seen from Table 4 that the total flavonoids yield is highest when the solid-liquid ratio is 1:50, so the solid-liquid ratio is selected as 1g:50mL.

[0100] Test Example 2

[0101] Based on the extraction process of Examples 1-6, the extraction rate of total flavonoids in Castanopsis altissima cake was determined using the above-mentioned determination process.

[0102] Table 5 Extraction rate of total flavonoids from different treatments of Castanopsis chinensis cake

[0103] sample Extraction rate (%) Example 1 4.79％ Example 2 3.97％ Example 3 4.32％ Example 4 4.01％ Example 5 4.26％ Example 6 4.47％

[0104] By comparing the data of Examples 1-6, it was found that the yield of total flavonoids from the Castanopsis schrenkiana cake prepared in Example 1 was significantly higher than that of the Castanopsis schrenkiana cake in Examples 2-6, and the yield of total flavonoids from the Castanopsis schrenkiana cake prepared in Experimental Examples 1-4 gradually increased under the optimization of experimental conditions, indicating that ultrasonic time, ultrasonic power and ultrasonic time as well as ethanol concentration have a significant effect on the extraction of total flavonoids from the Castanopsis schrenkiana cake.

[0105] In summary, it can be seen that the organic solvent concentration and parameter conditions such as ultrasound and centrifugation set in the technical scheme of the present invention can better promote the release of bioactive substances in Castanopsis altissima cake, shorten the extraction time, and effectively improve the purity and extraction rate of total flavonoids; at the same time, the extraction process is stable in operation and low in cost, and can be better applied to the development and utilization of Castanopsis altissima cake flavonoids.

[0106] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for extracting total flavonoids from Castanopsis schrenkiana cake, characterized in that: The steps include: (1) crushing and drying the tung oil tree cake obtained after the pretreatment to obtain tung oil tree cake powder; (2) mixing the ashwagandha seed cake powder with an organic solvent, performing ultrasonic treatment and separation to obtain a ashwagandha seed cake powder total flavonoids solution; (3) The total flavonoids solution of the ash cake powder is subjected to rotary evaporation and concentration to obtain the flavonoids solution.

2. The extraction method according to claim 1, characterized in that The ash cake in step (1) is crushed and passed through a sieve with a mesh size of 30-50 meshes.

3. The extraction method according to claim 1, characterized in that The drying temperature in step (1) is 50-70°C.

4. The extraction method according to claim 1, characterized in that The organic solvent in step (2) comprises ethanol, and the mass concentration of the ethanol is 70-90%; The solid-liquid ratio of the mixture of the Castanopsis schrenkiana cake powder and the organic solvent is 1g: (30-60)mL.

5. The extraction method according to claim 1, characterized in that In step (2), the power of the ultrasound is 300-500W, the temperature of the ultrasound is 50-80°C, and the time of the ultrasound is 50-70min.

6. The extraction method according to claim 1, characterized in that The separation in step (2) is carried out by centrifugation, the speed of the centrifugation is 7000-8500 r / min, and the time of the centrifugation is 5-15 min.

7. The extraction method according to claim 1, characterized in that Step (3) is concentrated to 1 / 20-1 / 5 of the volume of the total flavonoids solution of the Castanopsis schrenkiana cake powder.

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