Kudzuvine root seedling raising method

Abstract

The invention discloses a kudzu vine root seedling raising method. The disclosed method comprises the following steps: sequentially soaking kudzu vine root cuttings in a carbendazim solution and a growth solution; the growth solution is a mixed solution of growth hormone and an aqueous extract of fragrant eupatorium herb; the radix puerariae cuttage branches are lignified kudzu vines with bud nodes; performing cuttage on the soaked cuttings in a rooting medium for culture; the temperature of the culture environment is 26-30 DEG C, and the humidity is 76%-82%; when the cuttings are grown and buds grow to 5cm, applying a nutrient solution to the roots; and continuing to culture until the cutting wood roots and the root hair grows to 10cm to obtain the kudzu vine root seedlings. According to the method, the culture mode, the culture medium and the culture device are improved, so that the root primordium forming number of the cuttings is effectively increased, the rooting rate is increased, and the seedling raising period is shortened.

Description

Technical Field

[0001] The present invention relates to the technical field of kudzu seedling cultivation, and particularly relates to a kudzu seedling cultivation method. Background Art

[0002] Pueraria root, also known as kudzu vine, kudzu vine, and powder kudzu, is a vine plant of the genus kudzu of the leguminous family. It is rich in starch and is one of the first approved medicinal and edible plants. It is known as "northern ginseng and southern kudzu" and "Asian ginseng". Modern pharmacological studies have shown that the main medicinal ingredients in kudzu root are isoflavones such as puerarin, which have important application value in the field of modern medicine and health care, including lowering blood sugar, lowering blood pressure, lowering blood lipids, anti-arrhythmia, improving brain circulation and brain metabolism, sobering up, preventing osteoporosis, anti-inflammatory, anti-infection, anti-tumor, estrogen-like effects, improving memory, regulating endocrine and immunity, etc. However, due to the decreasing natural resources of wild kudzu, artificial cultivation has become the main way to obtain wild kudzu, and the production of wild kudzu seedlings has become a key link restricting the cultivation of wild kudzu, which is of great significance to the healthy development of the kudzu industry.

[0003] Traditional kudzu root cutting propagation is to select perennial woody kudzu vines as cutting materials for substrate cuttings. Cuttings have the problems of low rooting rate and long seedling cycle. Summary of the invention

[0004] In view of the defects or shortcomings of the prior art, the present invention provides a method for raising kudzu seedlings.

[0005] To this end, the Pueraria lobata seedling raising method provided by the present invention comprises:

[0006] The kudzu root cuttings are sequentially soaked in a carbendazim solution and a growth solution; the growth solution is a mixture of growth hormone and water extract of spatholobi; the kudzu root cuttings are lignified kudzu vines with bud nodes; the growth hormone is selected from one or a mixture of two or more of NAA, IBA and IAA;

[0007] The soaked cuttings are inserted into a rooting medium for cultivation; the cultivation environment temperature is 26-30°C and the humidity is 76%-82%; when the cuttings sprout and the buds grow to 3-6 cm, nutrient solution is applied to the roots; and then the cuttings are continuously cultivated until the roots grow to 10 cm to obtain kudzu seedlings;

[0008] The nutrient solution is prepared from a nutrient agent and water, and the nutrient agent is made from the following raw materials in parts by mass: 1 to 3 parts by mass of Bacillus amyloliquefaciens agent, 3 to 8 parts by mass of KNO3, 1 to 5 parts by mass of Ca(NO3)2, 1 to 5 parts by mass of MgSO4, and 1 to 6 parts by mass of KH2PO4.

[0009] Optionally, the lengths of the cuttings on both sides of the bud node of the Pueraria root cuttings are 7.2-7.8 cm and 2.4-2.8 cm respectively; and the longer side is the end where the root is located.

[0010] Optionally, the method for preparing the water extract of the airplane grass is to soak the dried airplane grass in water, heat and boil for 30 to 60 minutes, and then collect the filtrate, wherein the mass ratio of water to dried airplane grass is 1:(9 to 11).

[0011] Optionally, the growth liquid contains 1 to 4 parts by mass of growth hormone and 1 to 2 parts by mass of the Herba Lycopodii extract.

[0012] Optionally, the rooting medium is a mixture of vermiculite and perlite after absorbing water.

[0013] Optionally, the ratio of water to nutrient agent is 1000mL: (1-3)g.

[0014] The present invention improves the culture method, culture medium and culture device to effectively increase the number of root primordia formed by cuttings, improve the rooting rate and shorten the seedling raising period. In addition, the present invention uses the airplane grass extract as the raw material of the rooting liquid to promote better rooting and germination of kudzu cuttings. When the airplane grass extract is used in combination with hormones, the hormone effect can be enhanced and adjusted to make kudzu root grow better. DETAILED DESCRIPTION

[0015] Unless otherwise specified, the scientific and technical terms used herein are understood according to the knowledge of ordinary technicians in the relevant fields.

[0016] Euphorbia hirta (scientific name: Euphorbia hirta) is widely used in medicine. According to existing relevant research, it has anti-inflammatory, antioxidant, antimicrobial, antitumor, immunomodulatory and insect repellent activities. Gynostemmapentaphyllum (scientific name: Gynostemmapentaphyllum) is a traditional Chinese medicinal material. Studies have found that it has antioxidant, immunomodulatory, anti-inflammatory, anti-diabetic, cardiovascular protection, anti-tumor, antibacterial and neuroprotective effects. Seaweed extract has antioxidant, anti-inflammatory, antimicrobial, immunomodulatory, lipid-lowering, blood sugar-lowering, anti-tumor, obesity prevention and anti-diabetic and antiviral effects. Based on the overall scheme of the present invention, the present invention further selected the water extract of Euphorbia hirta, the water extract of Gynostemma pentaphyllum and the water extract of seaweed as growth-promoting substances for research, and found that the water extract of Euphorbia hirta has a more prominent synergistic effect on growth hormone.

[0017] The rooting medium of the present invention is made of vermiculite and perlite. The vermiculite plays a role of absorbing and retaining water after water is added, and can provide sufficient water for the growth of the kudzu root during the seedling raising process; the perlite has a supporting and foiling effect on the vermiculite, and prevents the vermiculite from being lost along the bottom holes of the seedling raising tray. Meanwhile, the micropores in the perlite can also make the seedling raising medium air permeable and water permeable, and the gap effect of the seedling raising and rooting in the medium is good, which is beneficial to promoting the root growth of the kudzu root.

[0018] The present invention is further explained below with reference to the embodiments.

[0019] The specific extraction method of the water extract of the airplane grass used in the following examples is: soak the dried airplane grass in water for 30 minutes, heat it to a slight boil for 60 minutes, and then collect the filtrate, wherein the mass ratio of water to dried airplane grass is 1:10.

[0020] The specific extraction method of the Gynostemma pentaphyllum water extract used is: soak the dried Gynostemma pentaphyllum in water for 30 minutes, heat it to a slight boil for 60 minutes, and then collect the filtrate, wherein the mass ratio of water to dried Gynostemma pentaphyllum is 1:10.

[0021] The seaweed water extraction preparation method used is: soak the dried seaweed in water for 30 minutes, heat to a slight boil for 60 minutes, and then collect the filtrate, wherein the mass ratio of water to dried seaweed is 1:10.

[0022] The hormone used in the following examples is NAA (naphthaleneacetic acid), which belongs to the auxin plant regulator. At a suitable concentration, NAA can promote the production of adventitious roots. Other growth hormones that can achieve similar functions include IBA, IAA, etc.

[0023] Embodiment 1:

[0024] In this embodiment, cuttings are selected from early December, 60-70 mm thick, lignified, free of pests and diseases, undamaged, healthy and strong, and within 1.2 m from the root of Pueraria lobata vines. The vines are trimmed into small sections with bud nodes and a length of 7.2-7.8 cm at the lower end of the bud node and a length of 2.4-2.8 cm at the upper end of the bud node as cuttings; and the bottom (morphological lower end) of the spare cutting branches is cut at 45° for standby use.

[0025] First, the cuttings were immersed in a 50% carbendazim solution for 24 hours, and the bud nodes were prevented from contacting the carbendazim solution during the soaking process; after soaking, the cuttings were taken out and drained, and then soaked in a growth solution for 60 minutes, wherein the growth solution used was a mixture of 3 parts by weight of NAA and 1.5 parts by weight of an extract of the airplane grass;

[0026] Next, the lower end of the soaked cutting strip is inserted into a container containing a rooting medium (in this embodiment, the rooting medium is vermiculite and perlite with a particle size of 1-3 mm; the mass ratio of vermiculite to perlite is 2:1; the rooting container has a through hole at the bottom, and the rooting container is placed in a tray with water, and the water surface is 1-2 cm high, so that the rooting medium remains moist), the cutting depth is 2-2.5 cm, and the cutting strip is inserted vertically, and only one cutting strip is inserted into each rooting container to ensure that each cutting strip has sufficient growth space; during the cutting process, an automatic spray system is used to control air humidity and substrate moisture;

[0027] After cutting, place the cuttings in a culture room at a temperature of 26-30°C and a humidity of 76%-82%;

[0028] When the kudzu vine sprouts and grows to 5cm, apply nutrient solution to the roots (10 days / time, 100ml per plant each time, where the ratio of nutrient solution to water is 1g:1000mL), and cut off the side branches in time during the process; continue to cultivate until roots take root and the roots grow to 10cm to obtain seedlings;

[0029] The nutrient used in this embodiment is made of the following raw materials: 2 parts by mass of Bacillus amyloliquefaciens agent (Shaanxi Guangren Biotechnology Co., Ltd., registration certificate number: Microbial Fertilizer (2018) Standard No. (3654)), 6 parts by mass of KNO3, 3 parts by mass of Ca(NO3)2, 4 parts by mass of MgSO4, and 6 parts by mass of KH2PO4.

[0030] Comparative Example 1:

[0031] The difference between this comparative example and Example 1 is that no growth liquid is used for immersion, and other operations are the same as those in Example 1.

[0032] Comparative Example 2:

[0033] The difference between this comparative example and Example 1 is that the Herba Lysimachiae extract in the growth liquid is replaced with seaweed extract, and the other operations are the same as Example 1.

[0034] Comparative Example 3:

[0035] The difference between this comparative example and Example 1 is that the Herba Gynostemmaesaes extract in the growth liquid is replaced with the Gynostemma pentaphyllum extract, and the other operations are the same as those in Example 1.

[0036] Comparative Example 4:

[0037] The difference between this comparative example and Example 1 is that the Herba Lysimachiae extract in the growth liquid is replaced with distilled water, and the other operations are the same as Example 1.

[0038] Comparative Example 5:

[0039] The difference between this comparative example and Example 1 is that the growth hormone in the growth solution is replaced by distilled water, and the other operations are the same as Example 1.

[0040] The above embodiments and comparative examples were all conducted in three parallel experimental groups at the same time, and the conditions of cultivating kudzu root by the above groups were investigated and recorded, and the rooting conditions (the number of roots and the root length were both average values) were recorded as shown in Table 1. The rooting rate, the number of roots and the root length of each group in the table were all statistically obtained on the 25th day of cutting, wherein the rooting rate = the number of rooting spikes / the total number of spikes × 100%; the time required for rooting is the time required for the root to grow to 10 cm. During the measurement, the root length and the number of roots in each experimental group were measured by technicians using a caliper of corresponding accuracy and then averaged and calculated.

[0041] Table 1 Rooting of kudzu vine

[0042]

[0043]

[0044] From the test results in Table 1, it can be seen that the airplane grass extract has a good promoting effect on the rooting of kudzu, which is better than the rooting effect of adding only a single hormone. It effectively improves the rooting rate of kudzu, and the number of rooted plants and the longest root length are also higher than the single hormone group, which accelerates the rooting time of kudzu, and rooted kudzu seedlings can be obtained in about 15 days.

[0045] In addition, the extract of airplane grass has a very good promoting effect on the rooting of kudzu, which is better than the rooting effect of seaweed extract and Gynostemma pentaphyllum extract, effectively improving the rooting rate of kudzu. From the data, it can be seen that the effect of airplane grass extract + hormone is greater than the effect of seaweed extract + hormone is greater than the effect of Gynostemma pentaphyllum extract + hormone.

Claims

1. A method for raising kudzu root seedlings, characterized in that: include: Soak the kudzu root cuttings in carbendazim solution and growth solution in turn; The growth liquid is a mixture of growth hormone and water extract of kudzu vine; the kudzu root cuttings are lignified kudzu vines with bud nodes; the growth hormone is selected from one or a mixture of two or more of NAA, IBA and IAA; The soaked cuttings are inserted into a rooting medium for cultivation; the cultivation environment temperature is 26-30°C and the humidity is 76%-82%; when the cuttings sprout and the buds grow to 3-6 cm, nutrient solution is applied to the roots; and then the cuttings are continuously cultivated until the roots grow to 10 cm to obtain kudzu seedlings; The nutrient solution is prepared from a nutrient agent and water, and the nutrient agent is made from the following raw materials in parts by mass: 1 to 3 parts by mass of Bacillus amyloliquefaciens agent, 3 to 8 parts by mass of KNO3, 1 to 5 parts by mass of Ca(NO3)2, 1 to 5 parts by mass of MgSO4, and 1 to 6 parts by mass of KH2PO4.

2. The method for raising kudzu seedlings according to claim 1, wherein The lengths of the cuttings on both sides of the bud node of the kudzu root cuttings are 7.2-7.8 cm and 2.4-2.8 cm respectively; and the longer side is the end where the root is located.

3. The method for raising kudzu seedlings according to claim 1, characterized in that: The method for preparing the water extract of the airplane grass is as follows: soaking the dried airplane grass in water, heating and boiling for 30 to 60 minutes, and then collecting the filtrate, wherein the mass ratio of water to the dried airplane grass is 1:(9 to 11).

4. The method for raising kudzu seedlings according to claim 3, characterized in that: The growth liquid contains 1 to 4 parts by weight of growth hormone and 1 to 2 parts by weight of the Herba Lycopodii extract.

5. The method for raising kudzu seedlings according to claim 1, characterized in that: The rooting medium is a mixture of vermiculite and perlite after absorbing water.

6. The method for raising kudzu seedlings according to claim 1, characterized in that: The ratio of water to nutrient agent is 1000mL: (1-3)g.

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