Composition for preventing hair loss, controlling oil and removing dandruff and preparation method thereof

Abstract

The invention discloses a composition for preventing hair loss, controlling oil and removing dandruff and a preparation method thereof, and particularly belongs to the field of preparations for dressing and makeup. The composition for preventing hair loss, controlling oil and removing dandruff comprises the following components: 2-5 parts of a folium artemisiae argyi extract, 1.1-1.6 parts of a ginger root extract, 5-10 parts of a polygonum multiflorum extract and 4-6 parts of a cacumen biotae extract. The polygonum multiflorum extract prepared by the method disclosed by the invention has better bacteriostatic activity, and compared with single strain fermentation, the mixed strain fermentation can more effectively enrich active ingredients in polygonum multiflorum; the anti-hair-loss, oil-controlling and anti-dandruff composition provided by the invention has excellent oil-controlling and oil-inhibiting effects; the shampoo provided by the invention has remarkable effects of preventing hair loss and strengthening hair.

Description

Technical Field

[0001] The invention belongs to the field of cosmetic preparations, and particularly relates to an anti-hair loss, oil control and anti-dandruff composition and a preparation method thereof. Background Art

[0002] Hair loss, excessive scalp oil secretion and dandruff are scalp health issues that consumers are currently concerned about. These problems not only affect personal image, but may also have a negative impact on personal psychology and social interactions.

[0003] First of all, there are many causes of hair loss, including physiological shedding and pathological shedding. Physiological shedding is a normal metabolic process of hair, and the amount of hair lost every day is normal within a certain range. However, when hair falls out excessively and exceeds the normal range, pathological hair loss occurs. The causes of pathological hair loss are complex and varied, including genetics, mental stress, physical and chemical stimulation, infection, endocrine disorders, malnutrition, and seasonal changes. These causes damage to hair follicles, which in turn affects the normal growth and development of hair.

[0004] Secondly, excessive scalp oil secretion is also a common problem. The scalp has abundant sebaceous glands, and excessive secretion of sebum will stimulate the accelerated keratinization of the scalp and form excessive dandruff. At the same time, excessive sebum may also clog the hair follicles, affecting the normal breathing and nutrient absorption of the hair, further aggravating the problem of hair loss.

[0005] Furthermore, dandruff is also a common concern for consumers. The occurrence of dandruff is related to the imbalance of the scalp microbiome, especially the overgrowth of Malassezia. Malassezia is a lipophilic fungus that uses the sebum secreted by the scalp as a source of nutrition. When it overgrows, it will stimulate the scalp to produce an inflammatory response, leading to the occurrence of dandruff.

[0006] In order to solve these problems, various anti-hair loss, oil control and anti-dandruff products have appeared on the market. However, these products often have problems such as insignificant effects, high irritation, and easy dependence. Therefore, it is particularly important to develop a product that has significant anti-hair loss, oil control and anti-dandruff effects and is safe, mild and non-irritating. Summary of the invention

[0007] In view of the deficiencies in the prior art, the present invention aims to provide a shampoo having significant anti-hair loss, oil control and anti-dandruff effects and being safe, mild and non-irritating.

[0008] In order to achieve the above object, the present invention discloses the following technical solutions:

[0009] In a first aspect, the present invention provides an anti-hair loss, oil control and anti-dandruff composition, which comprises the following components in parts by mass:

[0010] 2-5 parts of Artemisia argyi extract;

[0011] Ginger root extract 1.1-1.6 parts;

[0012] 5-10 parts of Polygonum multiflorum extract;

[0013] 4-6 parts of Platycladus orientalis leaf extract;

[0014] The preparation method of the Polygonum multiflorum extract comprises the following steps:

[0015] S1-1. The Polygonum multiflorum was crushed and sieved to obtain a Polygonum multiflorum powder, and the Polygonum multiflorum powder was mixed with deionized water at a solid-liquid ratio of 1:5 g / mL, heated to 80-90 ° C and kept warm for 10 h, and then cooled to room temperature to obtain a solid-liquid mixture;

[0016] S1-2. Take Bacillus subtilis and inoculate it in the seed medium, incubate it at a speed of 100-125r / min in a shaker at 28-30 ℃ for 24-36h to obtain a Bacillus subtilis culture solution;

[0017] S1-3. Take Bacillus Velez inoculated in the seed medium, incubate at a speed of 100-125r / min in a shaker, 28-30 ℃ for 24-36h to obtain Bacillus Velez bacteria solution;

[0018] S1-4. The Bacillus subtilis solution and the Bacillus Velez solution were mixed in a volume ratio of (1.8-2): 1 to obtain a mixed solution, the mixed solution was mixed with the solid-liquid mixture at a ratio of 1:10 mL / g, and fermented at a speed of 200-300 r / min, a temperature of 30±2°C, and a ventilation volume of 1.5-2 vvm for 24 hours. After the fermentation was completed, the mixture was centrifuged, filtered to remove the residue, the supernatant was taken, and low-temperature instantaneous sterilization was performed. Finally, the supernatant was spray-dried to obtain the Polygonum multiflorum extract.

[0019] Preferably, the seed culture medium is prepared by: preparing 5.0 g / L peptone, 3.0 g / L beef extract, 5.0 g / L NaCl, 0.005 g / L MnSO4·H2O and 1 L sterile water, sterilizing by high pressure at 121° C. for 15 min, cooling and setting aside.

[0020] Preferably, the viable count of the Bacillus subtilis liquid is ≥ 10 6 CFU / mL;

[0021] Preferably, the viable count of the Bacillus Velezii liquid is ≥ 10 6 CFU / mL.

[0022] Preferably, in the step S1-1, the Polygonum multiflorum is crushed and then passed through a 60-100 mesh sieve.

[0023] Preferably, in step S1-4, after the fermentation is completed, the mixture is centrifuged at 8000 r / min for 25 min to filter out the residue and obtain the supernatant.

[0024] In a second aspect, the present invention provides the use of the anti-hair loss, oil control and anti-dandruff composition of the first aspect in the preparation of a hair care product with anti-hair loss, oil control, anti-dandruff and hair growth effects.

[0025] In a third aspect, the present invention provides an anti-hair loss, oil control and anti-dandruff shampoo, wherein the shampoo comprises the anti-hair loss, oil control and anti-dandruff composition according to the first aspect;

[0026] The amount of the anti-hair loss, oil control and anti-dandruff composition added to shampoo is 0.5-2wt%;

[0027] The shampoo also contains surfactants, conditioners, preservatives, moisturizers, fragrances, anti-dandruff agents, thickeners, pH regulators, colorants, cooling agents and solvents.

[0028] Preferably, the surfactant is at least one of sodium laureth sulfate, cocamidopropyl betaine, sodium lauryl sulfate, cocamide MEA, quaternium-73, polysorbate-20, polyquaternium-52, laureth-16, TEA dodecylbenzenesulfonate, guar hydroxypropyltrimonium chloride, and C12-15 parathion-3;

[0029] The conditioning agent is at least one of trihydroxystearin, hydrolyzed chitosan, hydrolyzed corn starch, dimethicone alcohol, and dimethicone;

[0030] At least one of the preservatives p-hydroxyacetophenone, phenoxyethanol, and sodium benzoate;

[0031] The moisturizer is at least one of 1,2-hexanediol, butylene glycol, glycerin, caprylyl glycol, propylene glycol, and coconut oil;

[0032] The fragrance is a daily fragrance;

[0033] The anti-dandruff agent is piroctone olamine salt;

[0034] The thickener is at least one of sodium chloride and acrylic acid (ester) / acrylamide copolymer;

[0035] The pH regulator is at least one of citric acid and ethanolamine;

[0036] The colorant is at least one of CI 15985, CI 16255, and CI 42090;

[0037] The cooling agent is menthol;

[0038] The solvent is deionized water.

[0039] In a fourth aspect, the present invention provides a method for preparing the anti-hair loss, oil control and anti-dandruff shampoo according to the third aspect, characterized in that it comprises the following steps:

[0040] The anti-hair loss, oil control and anti-dandruff composition, surfactant, conditioner, preservative, moisturizer, fragrance, anti-dandruff agent, thickener, colorant, coolant and solvent are stirred and mixed evenly to obtain the anti-hair loss, oil control and anti-dandruff shampoo.

[0041] In the present invention:

[0042] Artemisia argyi extract contains rich active ingredients such as volatile oils, flavonoids, tannins, polysaccharides, triterpenes, trace elements and chlorogenic acid. Volatile oils have anti-asthmatic and antibacterial effects, flavonoids, chlorogenic acid and sesquiterpenoids have anti-inflammatory effects, tannins have astringent and hemostatic effects. At the same time, artemisia argyi extract has a strong antibacterial effect on Malassezia, Escherichia coli and Staphylococcus aureus.

[0043] Ginger root extract is rich in gingerol, shogaol, gingerol and zingerone, as well as amino acids, etc. It has antibacterial, soothing, anti-inflammatory and antioxidant effects. At the same time, ginger root extract can promote blood circulation in the scalp, promote the active growth of hair follicle cells, assist hair growth, and have a certain regulatory effect on the oil secretion of the scalp.

[0044] Polygonum multiflorum extract contains active ingredients such as anthraquinone compounds, distilbene glycoside compounds and phospholipid compounds, which can promote blood circulation in the scalp, promote the active growth of hair follicle cells, and thus promote hair growth. At the same time, it has certain anti-inflammatory and antibacterial effects.

[0045] Platycladus orientalis leaf extract is rich in volatile oils, flavonoids, tannins and other active substances, and has antibacterial, anti-tumor, anti-inflammatory, anti-red blood cell oxidation and hemostatic effects, especially in preventing hair loss. The volatile oil in Platycladus orientalis leaf extract has a significant inhibitory effect on Malassezia, Staphylococcus aureus, Tetragenous cocci, Escherichia coli and Bacillus aerogenes. Platycladus orientalis leaf extract can remove dandruff, promote hair regeneration, enhance the metabolic function of hair follicles, and has a certain regulatory effect on scalp oil secretion.

[0046] Microbial fermentation technology can effectively enrich the active ingredients in Polygonum multiflorum and enhance its pharmacological activity. The present invention has found that by fermenting Polygonum multiflorum with a mixed strain of Bacillus subtilis and Bacillus velezensis, the physiological activities of Polygonum multiflorum extract, such as antioxidant, anti-inflammatory, antibacterial, and oil control, can be significantly increased. During the fermentation process, the content of anthraquinone substances and diphenylethylene glycosides will increase, and new anthraquinone components will be produced by microbial metabolism.

[0047] Beneficial effects of the present invention:

[0048] 1. The Polygonum multiflorum extract prepared by the method of the present invention has good antibacterial activity, and mixed strain fermentation can more effectively enrich the active ingredients in Polygonum multiflorum than single strain fermentation.

[0049] 2. The anti-hair loss, oil control and anti-dandruff composition provided by the present invention has excellent oil control and oil inhibition effects.

[0050] 3. The shampoo provided by the present invention has significant effects of preventing hair loss and strengthening hair. DETAILED DESCRIPTION

[0051] In order to make the purpose, technical scheme and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments. However, the following embodiments are only preferred embodiments of the present invention, not all. For the sake of clarity, all features of the actual embodiments are not described.

[0052] Based on the embodiments in the implementation manner, other embodiments obtained by those skilled in the art without making any creative work shall all fall within the protection scope of the present invention.

[0053] In the present invention:

[0054] Artemisia alginate leaf extract: Artemisia alginate leaf extract, purchased from Xi'an Shennong Biotechnology Co., Ltd., specification 20:1;

[0055] Ginger root extract: Zingiber officinale root extract, purchased from Xi'an Shennong Biotechnology Co., Ltd., specification 20:1;

[0056] Polygonum multiflorum extract: Polygonum multiflorum (POLYGONUM MULTIFLORUM) extract, dried Polygonum multiflorum was purchased from Guangzhou Qingping Medicinal Materials Wholesale Market;

[0057] Oriental Platycladus orientalis leaf extract: Oriental Platycladus orientalis leaf extract, purchased from Xi'an Shennong Biotechnology Co., Ltd., specification 20:1;

[0058] Bacillus subtilis: purchased from Guangdong Microbiological Culture Collection Center, with the collection number GDMCC NO.1.131;

[0059] Bacillus Velezii: purchased from Guangdong Microbiological Culture Collection Center, with the collection number GDMCC NO.1.135;

[0060] Other raw materials are commercially available.

[0061] Preparation of Polygonum multiflorum extract:

[0062] S1-1. Take dried Polygonum multiflorum and crush it in a high-speed grinder, pass through a 60-mesh sieve to obtain Polygonum multiflorum powder, and mix the Polygonum multiflorum powder with deionized water at a solid-liquid ratio of 1:5g / mL, heat it to 90 ° C and keep it for 10h, and then cool it to room temperature to obtain a solid-liquid mixture;

[0063] S1-2. Use an inoculation loop to scrape 0.5 cm 2 The Bacillus subtilis strain was inoculated into the prepared seed culture medium and cultured at 30°C for 30 h in a shaking incubator at a speed of 125 r / min to obtain a Bacillus subtilis bacterial solution with a viable cell count of 5 × 10 6 CFU / mL;

[0064] S1-3. Use an inoculation loop to scrape 0.5 cm 2 The slant of Bacillus Velezii was inoculated into the prepared seed culture medium and cultured at 28°C for 36 h in a shaking incubator at a speed of 125 r / min to obtain a Bacillus Velezii bacterial solution with a viable cell count of 5 × 10 6 CFU / mL;

[0065] S1-4. The Bacillus subtilis culture and the Bacillus Velez culture were mixed at 2:1 v / v to obtain a mixed culture, and the mixed culture was mixed with the solid-liquid mixture obtained in step S1-1 at 1:10 mL / g and placed in a fermenter, and fermented at a speed of 200 r / min, a temperature of 30 ± 2 ° C, and a ventilation volume of 2vvm for 24 h. After the fermentation was completed, the mixture was centrifuged at 8000 r / min for 25 min, filtered to remove the residue, and the supernatant was subjected to low-temperature instantaneous sterilization, and finally the supernatant was spray-dried to obtain a Polygonum multiflorum extract ①;

[0066] The preparation method of the seed culture medium is as follows: prepare it according to 5.0 g / L of peptone, 3.0 g / L of beef extract, 5.0 g / L of NaCl, 0.005 g / L of MnSO4·H2O and 1 L of sterile water, sterilize it under high pressure at 121°C for 15 min, cool it and set it aside.

[0067] In order to prove the excellent properties of the above-prepared Polygonum multiflorum extract, the following Polygonum multiflorum extracts obtained by different preparation methods and commercially available Polygonum multiflorum extracts are provided and numbered for easy distinction.

[0068] Polygonum multiflorum extract ②: refer to steps S1-1, S1-2, and S1-3, and modify step S1-4 to mix the Bacillus subtilis solution and the Bacillus velezensis solution at a ratio of 1.8:1 v / v. The remaining steps and parameters are the same.

[0069] Polygonum multiflorum extract ③: Take dried Polygonum multiflorum and grind it in a high-speed grinder, pass it through a 60-mesh sieve to obtain Polygonum multiflorum powder, mix the Polygonum multiflorum powder with deionized water at a solid-liquid ratio of 1:5 g / mL, heat it to 90°C and keep it warm for 10 hours, then cool it to room temperature to obtain a solid-liquid mixture, centrifuge it at 8000 r / min for 25 minutes, filter out the residue, take the supernatant for low-temperature instantaneous sterilization, and then spray-dry it to obtain Polygonum multiflorum extract ③.

[0070] Polygonum multiflorum extract ④: Referring to steps S1-1, S1-2, and S1-3, step S1-4 is modified to only mix and ferment the Bacillus subtilis solution with the solid-liquid mixture at a ratio of 1:10 mL / g, and the remaining steps and parameters are the same.

[0071] Polygonum multiflorum extract ⑤: Referring to steps S1-1, S1-2, and S1-3, step S1-4 is modified to only subject the Bacillus velezensis liquid and the solid-liquid mixture to mixed fermentation treatment at a ratio of 1:10 mL / g, and the remaining steps and parameters are the same.

[0072] Polygonum multiflorum extract ⑥: refer to steps S1-1, S1-2, and S1-3, and modify step S1-4 to mix the Bacillus subtilis solution and the Bacillus velezensii solution at a ratio of 1:1 v / v. The remaining steps and parameters are the same.

[0073] Polygonum multiflorum extract ⑦: refer to steps S1-1, S1-2, and S1-3, and proceed to step S1-4, modifying to mix the Bacillus subtilis culture solution and the Bacillus velezensii culture solution at a ratio of 3:1 v / v, and the remaining steps and parameters are the same.

[0074] Polygonum multiflorum extract⑧: refer to steps S1-1, S1-2, and S1-3, and modify step S1-4 to mix the Bacillus subtilis culture solution and the Bacillus natto culture solution at a ratio of 2:1 v / v. The remaining steps and parameters are the same.

[0075] Polygonum multiflorum extract ⑨: Referring to steps S1-1, S1-2, and S1-3, step S1-4 is modified to mix the Bacillus subtilis solution and the Bacillus licheniformis solution at a ratio of 2:1 v / v, and the remaining steps and parameters are the same.

[0076] Polygonum multiflorum extract ⑩: Polygonum multiflorum extract C-II purchased from Guangzhou Luojie Biotechnology Co., Ltd.

[0077] Among them, the Bacillus natto and Bacillus licheniformis bacterial liquids were prepared by conventional culture, and the number of viable cells was 5×10 6 CFU / mL.

[0078] Preparation of the anti-hair loss, oil control and anti-dandruff composition:

[0079] Weigh each raw material component according to the formula in Table 1, place them in a mixing and blending machine and mix them evenly to obtain the anti-hair loss, oil control and anti-dandruff composition.

[0080] Table 1 Raw materials and their weight fractions of the anti-hair loss, oil control and anti-dandruff composition

[0081]

[0082] Note: “-” in the table means no addition.

[0083] The above compositions 1-11 were mixed with deionized water to prepare sample solutions with a concentration of 2 wt % and then subjected to performance tests.

[0084] Performance test of the anti-hair loss, oil control and anti-dandruff composition:

[0085] 1 Malassezia antibacterial test

[0086] 1.1 Detection principle

[0087] Malassezia is one of the important factors in the formation of dandruff. The growth and decline of Malassezia is directly correlated with the amount of dandruff. Malassezia furfur is a relatively in-depth study of the bacteria in the genus Malassezia. It is isolated from human dandruff (pityriasis capitis) and is representative of indicator bacteria. In this test, a sample containing an antibacterial agent is placed on a plate stained with Malassezia furfur. The sample is continuously dissolved and diffused through agar to form different concentration gradients to show its antibacterial effect. By measuring the size of the antibacterial ring to determine whether it has antibacterial ability, it is suitable for the identification of antibacterial and dissolvable antibacterial (inhibitory) products.

[0088] 1.2 Criteria

[0089] The negative control sample should not produce an antibacterial ring. If the diameter of the antibacterial ring of the test sample is greater than 7mm, it is judged to have an antibacterial effect; if the diameter of the antibacterial ring is ≤7mm, it is judged to have no antibacterial effect.

[0090] 1.3 Test methods

[0091] Take sterile dry filter paper and add 20 μL of sample solution to each piece to obtain the test sample. For the negative control sample, replace the sample solution with sterile water. Use a sterile cotton swab to dip the sample with a concentration of 5.0×105 CFU / mL~5.0×10 6 CFU / mL Malassezia furfur suspension is evenly applied on the surface of the appropriate culture medium plate for 3 times. Place 1 infected plate for each test, 4 test samples and 1 negative control sample on each plate, for a total of 5 plates. Cover the plate, incubate at (36±1)℃ for 72h, observe the results, and measure the diameter of the inhibition ring (including the patch) with a vernier caliper and record it.

[0092] 1.4 Test results

[0093] Table 2 Antibacterial performance results of compositions 1-11

[0094]

[0095] 1.5 Results Analysis

[0096] According to the test results, no antibacterial rings were produced in the negative control samples, and the diameters of the antibacterial rings of the tested samples were all >7 mm, indicating that all samples have the ability to inhibit Malassezia furfur, among which the antibacterial performance of composition 2 is better than that of compositions 4-11, proving that the Polygonum multiflorum extract prepared by the method of the present invention has good antibacterial activity, and the specific strain composite fermentation provided by the present invention can more effectively enrich the active ingredients in Polygonum multiflorum than other strains or single strain fermentation.

[0097] 2 Oil control efficacy test

[0098] 2.1 Preparation of reagents

[0099] Samples to be tested: As described above, the compositions 1-11 prepared above were respectively taken to prepare sample solutions with a concentration of 2 wt%.

[0100] Testosterone (T) solution: Dissolve 36 mg of testosterone in anhydrous ethanol to prepare a testosterone solution with a concentration of 5 mmol / L;

[0101] NADPH (reduced coenzyme II) solution: Dissolve 42.5 mg of NADPH tetrasodium salt in water to prepare a 2 mmol / L NADPH solution;

[0102] Reaction solution: Dissolve DTT (dithiothreitol) and sodium phosphate in water to make the final concentrations of DTT and sodium phosphate 1 mmol / L and 20 mmol / L, respectively, to obtain a reaction solution.

[0103] 2.25 Determination of α-reductase activity

[0104] The reaction components were mixed in order (reaction solution 155 μL, constant temperature 37°C; testosterone solution 10 μL; NADPH solution 10 μL; 5α-reductase 20 μL), and the absorbance change of the reaction system at 340 nm was continuously monitored within 4 min. The rate of change reflected the activity of 5α-reductase (in a reaction system with a temperature of 37°C, the amount of enzyme that causes the NADPH concentration to decrease by 1 μmol / L per minute is one enzyme activity unit), which was recorded as the initial enzyme activity.

[0105] 2.35α-Reductase Inhibition Rate Determination

[0106] The reaction components were mixed in order (reaction solution 155 μL, constant temperature 37°C; testosterone solution 10 μL; NADPH solution 10 μL; sample solutions 15 μL corresponding to composition 1-11 were taken respectively; 5α-reductase 20 μL), and the change of the absorbance value of the reaction system at 340 nm was continuously monitored within 4 minutes. The enzyme activity of 5α-reductase inactivation was calculated with reference to the NADPH standard curve, and then the 5α-reductase inhibition rate was calculated according to "5α-reductase inhibition rate = inactivated enzyme activity / initial enzyme activity × 100%". The test results are shown in Table 3.

[0107] Table 3 Oil control effect results

[0108]

[0109]

[0110] 2.4 Results Analysis

[0111] 5α-reductase is a membrane protease that depends on reduced coenzyme II (NADPH). It is an important male hormone metabolic enzyme in the skin and can irreversibly convert testosterone into dihydrotestosterone. DHT is the most active male hormone and can induce excessive oil secretion from the sebaceous glands. Reducing the level of DHT by inhibiting the activity of 5α-reductase can effectively alleviate excessive oil secretion from the sebaceous glands.

[0112] According to the experimental results, compositions 1-3 provided by the present invention have excellent oil control effects, among which composition 2 has better oil control effects than compositions 1 and 3; according to the results of compositions 4-11, it is proved that the Polygonum multiflorum extract prepared by the method of the present invention has good oil control effects, and the Bacillus subtilis solution and the Bacillus velezensii solution in the specific mixed strains provided by the present invention have excellent oil control performance within a specific preparation range, and show better oil control efficacy than a single strain or a combination of other strains.

[0113] Preparation of Shampoo:

[0114] S2-1. Weighing: Accurately weigh each raw material component according to the formula in Table 1;

[0115] S2-2. Feeding and heating: Add the raw materials of phase A to the emulsifying pot, mix and stir and heat to 85 ° C, turn on the homogenizer, emulsify for 10 minutes, turn off the emulsifying homogenizer, continue stirring for 20 minutes until completely dissolved, keep warm for 30 minutes to defoam, and then start cooling;

[0116] S2-3. Cool down to 60°C, add the raw materials of phase B into the emulsifier and mix well, continue to cool down to 50°C, add the raw materials of phase C into the emulsifier in sequence and stir for 20 minutes until uniform, add the raw materials of phase D into the emulsifier in sequence at 45°C and stir well, take the materials for inspection, and discharge the qualified materials;

[0117] S2-4. Packaging: After discharging, let it stand for 24 hours. After passing the microbiological test, it is filled by a filling machine and packaged into finished products with inkjet coding;

[0118] S2-5. Finished product inspection: After the finished product is packaged, it will be subjected to physical, chemical and microbiological inspections. After passing the inspection, it will be sealed and put into storage.

[0119] Table 4 Shampoo raw materials and their mass percentage

[0120]

[0121]

[0122] Note: “-” in the table means no addition.

[0123] Human trials:

[0124] 1. Materials and Methods

[0125] 1. Trial materials: Example 1 and Comparative Example 1.

[0126] 2. Subjects: A total of 30 subjects, including 13 males and 17 females, aged 23 to 58 years old, with an average age of 40.5±11.5 years old, met the voluntary inclusion criteria.

[0127] 3. Trial method: Have the subjects directly use the test product according to the usage characteristics and methods indicated on the cosmetic product label, observe or follow up by phone once a week for the subjects' skin reactions, and record the results according to the grading standards for adverse skin reactions in human trial tests in the currently valid technical specifications. The trial period shall be no less than 4 weeks.

[0128] 2. Test results

[0129] Table 5 Human trial test results

[0130]

[0131] Result analysis: The results of the human trial showed that there were no adverse skin reactions among the 30 people.

[0132] Anti-shedding performance test:

[0133] 1. Materials and Methods

[0134] 1. Test substance: Example 1.

[0135] 2. Test product: Example 1.

[0136] 3. Subjects: A total of 30 people, experimental group: 1 male, 29 females, aged 21 to 58 years old, average age 44.3±10.9 years old, met the voluntary inclusion criteria of the subjects.

[0137] 4. Instruments and Equipment

[0138] 4.1 Hair Growth Management System, Model: DermoGeniusultra

[0139] 4.2 Digital camera, model: EOS850D

[0140] 5. Test methods:

[0141] Volunteer subjects were recruited according to the inclusion criteria, and the number of hair loss was counted using the 60-times combing method. Qualified subjects underwent a 2-week washout period, and after the washout period, the 60-times combing method was performed again. Those with more than 10 hair loss counts entered the formal trial. The selected subjects were evaluated for hair basal values ​​before using the product, including hair loss counts, hair density assessments, and image capture, and the results were recorded; the same evaluation and testing were performed again 4, 8, and 12 weeks after the product was used.

[0142] 6. Statistical methods:

[0143] Statistical analysis software was used for statistical analysis of the data. Measurement data were expressed as mean ± standard deviation, and normal distribution test was performed. If the data met the normal distribution requirements, the paired t test was used for comparison before and after, otherwise the two related sample rank sum test was used; the comparison of rank data before and after was performed, and the two related sample rank sum test was used. The above statistical analyses were all two-tailed tests, and the significance level was α = 0.05.

[0144] 2. Results and Data Analysis

[0145] Table 6 Analysis of time points before and after use of the test product

[0146]

[0147] 3. Test Conclusion

[0148] Compared with before use, there was no significant increase in hair loss counts in the test product group 4 weeks, 8 weeks, and 12 weeks after use, and the hair loss counts after 8 weeks and 12 weeks were significantly lower than those at 0 week (P < 0.05).

[0149] Compared with before use, the overall hair density of the test product group did not decrease significantly after 4 weeks, 8 weeks, and 12 weeks of use, and the overall hair density after 12 weeks of use was significantly higher than that at 0 weeks (P < 0.05).

[0150] Compared with before use, the local hair density of the test product group did not decrease significantly after 4 weeks, 8 weeks, and 12 weeks of use, and the local hair density after 4 weeks, 8 weeks, and 12 weeks of use was significantly higher than that at 0 week (P < 0.05). The test product has the effect of preventing hair loss.

[0151] Although the specific embodiments of the present invention are described above, it should be understood by those skilled in the art that this is only for illustration and the protection scope of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. A composition for preventing hair loss, controlling oil and removing dandruff, characterized in that: By mass, it includes the following components: 2-5 parts of Artemisia argyi extract; Ginger root extract 1.1-1.6 parts; 5-10 parts of Polygonum multiflorum extract; 4-6 parts of Platycladus orientalis leaf extract; The preparation method of the Polygonum multiflorum extract comprises the following steps: S1-1. The Polygonum multiflorum was crushed and sieved to obtain a Polygonum multiflorum powder, and the Polygonum multiflorum powder was mixed with deionized water at a solid-liquid ratio of 1:5 g / mL, heated to 80-90 ° C and kept warm for 10 h, and then cooled to room temperature to obtain a solid-liquid mixture; S1-2. Take Bacillus subtilis and inoculate it in the seed medium, incubate it at a speed of 100-125r / min in a shaker at 28-30°C for 24-36h to obtain a Bacillus subtilis culture solution; S1-3. Take Bacillus Velez inoculated in the seed medium, incubate at a speed of 100-125r / min in a shaker, 28-30 ℃ for 24-36h to obtain Bacillus Velez bacteria solution; S1-4. The Bacillus subtilis solution and the Bacillus Velez solution were mixed in a volume ratio of (1.8-2): 1 to obtain a mixed solution, the mixed solution was mixed with the solid-liquid mixture at a ratio of 1:10 mL / g, and fermented at a speed of 200-300 r / min, a temperature of 30±2°C, and a ventilation volume of 1.5-2 vvm for 24 hours. After the fermentation was completed, the mixture was centrifuged, filtered to remove the residue, the supernatant was taken, and low-temperature instantaneous sterilization was performed. Finally, the supernatant was spray-dried to obtain the Polygonum multiflorum extract.

2. The composition for preventing oil loss, controlling dandruff and removing dandruff according to claim 1, characterized in that: The seed culture medium is prepared by mixing 5.0 g / L peptone, 3.0 g / L beef extract, 5.0 g / L NaCl, 0.005 g / L MnSO4·H2O and 1 L sterile water, sterilizing by high pressure at 121° C. for 15 min, cooling and setting aside.

3. The composition for preventing oil loss, controlling dandruff and removing dandruff according to claim 1, characterized in that: The viable count of the Bacillus subtilis liquid is ≥10 6 CFU / mL.

4. The composition for preventing hair loss, controlling oil and removing dandruff according to claim 1, characterized in that: The viable count of the Bacillus Velezii liquid is ≥10 6 CFU / mL.

5. The composition for preventing hair loss, controlling oil and removing dandruff according to claim 1, characterized in that: In the step S1-1, the Polygonum multiflorum is crushed and then passed through a 60-100 mesh sieve.

6. The composition for preventing hair loss, controlling oil and removing dandruff according to claim 1, characterized in that: In step S1-4, after the fermentation is completed, the mixture is centrifuged at 8000 r / min for 25 min to filter out the residue and obtain the supernatant.

7. Use of the anti-hair loss, oil control and anti-dandruff composition according to any one of claims 1 to 6 in the preparation of a hair care product with anti-hair loss, oil control, anti-dandruff and hair growth effects.

8. An anti-hair loss, oil-control and anti-dandruff shampoo, characterized in that: The shampoo comprises the anti-hair loss, oil control and anti-dandruff composition according to any one of claims 1 to 6; The amount of the anti-hair loss, oil control and anti-dandruff composition added to shampoo is 0.5-2wt%; The shampoo also contains surfactants, conditioners, preservatives, moisturizers, fragrances, anti-dandruff agents, thickeners, pH regulators, colorants, cooling agents and solvents.

9. The anti-hair loss, oil-control and anti-dandruff shampoo according to claim 8, characterized in that: The surfactant is at least one of sodium laureth sulfate, cocamidopropyl betaine, sodium lauryl sulfate, cocamide MEA, quaternium-73, polysorbate-20, polyquaternium-52, laureth-16, TEA dodecylbenzenesulfonate, guar hydroxypropyltrimonium chloride, and C12-15 parathion-3; The conditioning agent is at least one of trihydroxystearin, hydrolyzed chitosan, hydrolyzed corn starch, dimethicone alcohol, and dimethicone; At least one of the preservatives p-hydroxyacetophenone, phenoxyethanol, and sodium benzoate; The moisturizer is at least one of 1,2-hexanediol, butylene glycol, glycerin, caprylyl glycol, propylene glycol, and coconut oil; The fragrance is a daily fragrance; The anti-dandruff agent is piroctone olamine salt; The thickener is at least one of sodium chloride and acrylic acid (ester) / acrylamide copolymer; The pH regulator is at least one of citric acid and ethanolamine; The colorant is at least one of CI 15985, CI 16255, and CI 42090; The cooling agent is menthol; The solvent is deionized water.

10. The method for preparing the anti-hair loss, oil-controlling and anti-dandruff shampoo according to claim 8 or 9, characterized in that: The following steps are involved: The anti-hair loss, oil control and anti-dandruff composition, surfactant, conditioner, preservative, moisturizer, fragrance, anti-dandruff agent, thickener, colorant, coolant and solvent are stirred and mixed evenly to obtain the anti-hair loss, oil control and anti-dandruff shampoo.

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