Traditional Chinese medicine composition for relieving asthenopia as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for relieving asthenopia and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 1-20 parts of schisandra chinensis, 1-20 parts of dogwood, 1-20 parts of rhizoma polygonati, 1-15 parts of glossy privet fruit, 1-15 parts of bighead atractylodes rhizome, 1-10 parts of white mulberry root-bark and 1-10 parts of chrysanthemum. The invention further provides a preparation method of the traditional Chinese medicine composition and application of the traditional Chinese medicine composition in preparation of drugs for relieving asthenopia. The traditional Chinese medicine composition disclosed by the invention has an obvious effect on the symptoms of dim vision, dry eyes, eye distension and eye and eye socket ache caused by asthenopia.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for relieving visual fatigue, and a preparation method and use thereof. Background Art

[0002] Visual fatigue is a common chronic disease among ophthalmic diseases, and its main manifestations include eye fatigue, dry eyes, foreign body sensation, heavy eyelids, blurred vision, eye pain and congestion. Visual fatigue is closely related to various eye diseases, especially myopia, presbyopia, cataracts and other diseases. Visual fatigue is a global problem, mostly caused by excessive use of the eyes, close use of the eyes, excessive radiation from electronic products and other reasons. At present, there are many kinds of health care products on the market for relieving visual fatigue, most of which use lutein, carotene and other preventive health care, but the effect is not good, and some imported products are sold at a high price.

[0003] Compared with the above-mentioned health products from Western medicine, traditional Chinese medicine has unique advantages in relieving visual fatigue. Unlike the above-mentioned health products from Western medicine, which generally choose a single ingredient or a simple combination of several ingredients, Chinese medicine prescriptions, based on dialectical reasoning, combine Chinese medicine theory and treatment principles, and use a variety of medicinal flavors to form a prescription to enhance or change the original efficacy of each medicinal flavor, eliminate or reduce its adverse factors on the human body, so as to achieve a complementary therapeutic effect. Visual fatigue belongs to the category of "liver fatigue" in traditional Chinese medicine ophthalmology. The eyes are the orifices of the liver, born in the kidneys, and used for the heart. Its pathogenesis is mainly related to the liver, heart, and kidneys. Visual fatigue is mostly caused by long-term vision, mental fatigue, spleen damage, or fatigue, liver and kidney essence and blood depletion, and inability to nourish the eyes. It is closely related to the spleen, liver, kidney, qi, and blood. Clinically, drugs that invigorate qi, strengthen the spleen, soothe the liver, tonify the kidney, and nourish blood are commonly used for prevention and treatment.

[0004] For example, the folk commonly used Chinese wolfberry, Schisandra, chrysanthemum, cassia seed, mulberry, longan pulp, dendrobium and other effects of calming the liver and clearing the heart, dispersing wind and clearing heat, strengthening the spleen and benefiting the lungs, regulating the liver and kidney, nourishing yin and improving eyesight are soaked in water instead of tea to drink, to clear the liver and improve eyesight, improve visual fatigue. In addition, there are some self-made prescriptions for relieving visual fatigue based on the above-mentioned folk commonly used medicines, for example, Chinese patent CN114796358B discloses a composition for relieving visual fatigue of liver and kidney yin deficiency type, which is made of the following raw materials in parts by weight: 6-8 parts of Cuscuta, 6-8 parts of Astragalus, 6-8 parts of Chinese wolfberry, 4-6 parts of Cornus, 4-6 parts of white peony, 4-6 parts of papaya, 6-8 parts of mulberry, 2-4 parts of Schisandra, 2-4 parts of Cistanche, the composition has the effect of nourishing the liver and kidney, benefiting the essence and improving eyesight, and also has a certain function of improving immunity. Chinese patent CN118436743B discloses a composition with the function of relieving visual fatigue, which is prepared from the following raw materials in weight ratio: 2.4-3.6 parts of wolfberry, 2.4-3.6 parts of chrysanthemum, 9.6-14.4 parts of polygonatum, 3.6-5.4 parts of corn silk, 4.8-7.2 parts of cornus, 3.6-5.4 parts of gardenia, 4.8-7.2 parts of yam, 3.6-5.4 parts of poria, and the composition can relieve visual fatigue caused by liver and kidney yin deficiency and spleen deficiency. However, the existing traditional Chinese medicine products are mostly piled up with commonly used traditional Chinese medicines with the effect of relieving visual fatigue from the perspective of formula, and generally do not use modern pharmacological methods to verify the efficacy of symptoms caused by visual fatigue. Therefore, there is still a lot of room for improvement in the efficacy of these products.

[0005] In view of this, the inventors conducted in-depth research on the existing traditional Chinese medicine prescriptions for relieving visual fatigue and improved them, and on this basis obtained a new traditional Chinese medicine composition for relieving visual fatigue with definite efficacy. Summary of the invention

[0006] In order to solve the problems that the existing traditional Chinese medicine prescriptions for relieving visual fatigue are not ideal in effect and are not reasonably compatible, the present invention provides a novel traditional Chinese medicine composition for relieving visual fatigue and a preparation method and use thereof. The traditional Chinese medicine composition of the present invention has obvious effects on symptoms such as blurred vision, dry eyes, swollen eyes, and soreness of eyes and orbits caused by visual fatigue.

[0007] Specifically, the present invention is realized through the following technical solutions:

[0008] In the first aspect, the present invention provides a traditional Chinese medicine composition for relieving visual fatigue, which is made of the following raw materials in parts by weight: 1-20 parts of Schisandra chinensis, 1-20 parts of Cornus officinalis, 1-20 parts of Polygonatum sibiricum, 1-15 parts of Ligustrum lucidum, 1-15 parts of Atractylodes macrocephala, 1-10 parts of Morus alba bark, and 1-10 parts of Chrysanthemum.

[0009] As an optional method, in the above-mentioned traditional Chinese medicine composition, the traditional Chinese medicine composition is made of the following raw materials in parts by weight: 1-15 parts of Schisandra chinensis, 1-15 parts of Cornus officinalis, 1-15 parts of Polygonatum sibiricum, 1-10 parts of Ligustrum lucidum, 1-10 parts of Atractylodes macrocephala, 1-8 parts of Morus alba bark, and 1-8 parts of Chrysanthemum.

[0010] As an optional method, in the above-mentioned traditional Chinese medicine composition, the traditional Chinese medicine composition is made of the following raw materials in parts by weight: 5-10 parts of Schisandra chinensis, 5-10 parts of Cornus officinalis, 5-10 parts of Polygonatum sibiricum, 4-7 parts of Ligustrum lucidum, 4-7 parts of Atractylodes macrocephala, 2-5 parts of Morus alba bark, and 2-5 parts of Chrysanthemum.

[0011] As an optional method, in the above-mentioned traditional Chinese medicine composition, the traditional Chinese medicine composition is made of the following raw materials in parts by weight: 7 parts of Schisandra chinensis, 7 parts of Cornus officinalis, 7 parts of Polygonatum sibiricum, 5 parts of Ligustrum lucidum, 5 parts of Atractylodes macrocephala, 2 parts of Morus alba bark, and 3 parts of Chrysanthemum.

[0012] In a second aspect, the present invention provides a method for preparing the Chinese medicine composition described in the first aspect, the preparation method comprising the following steps:

[0013] Weigh each Chinese medicinal material according to the weight proportions described in the first aspect above, decoct for the first time, cover the medicinal surface with cold water, and decoct after soaking, first boil over high heat, then decoct for a period of time over low heat, filter the residue and take the juice; decoct for the second time, cover the medicinal surface with cold water, decoct for a period of time after the water boils, filter the residue and take the juice, and mix and concentrate the two medicinal liquids.

[0014] As an optional manner, in the above preparation method, during the first decoction, the cold water covers the medicinal surface by about 3 cm, and the decoction is performed after soaking for 30 minutes, first boiling over high heat, and then decocting over low heat for 30-40 minutes. During the second decoction, the cold water covers the medicinal surface by about 2 cm, and the water is boiled for 20 minutes.

[0015] In a third aspect, the present invention provides use of the Chinese medicine composition described in the first aspect or the Chinese medicine composition prepared by the preparation method described in the second aspect in preparing a drug for relieving visual fatigue.

[0016] As an optional mode, in the above use, the drug is used to relieve the symptoms of blurred vision, dry eyes, swollen eyes, and sore eyes and orbits caused by visual fatigue.

[0017] As an optional mode, in the above use, the drug is used to relieve dry eyes caused by visual fatigue.

[0018] The drug of the present invention can be prepared by conventional preparation methods in the art, and pharmaceutically acceptable excipients may or may not be added. As long as the Chinese medicine raw material is the above weight portion, regardless of the various changes in its preparation method, it belongs to the scope of the present invention. Its drug form includes but is not limited to solid form, semi-solid form or liquid form, etc.; specifically includes but is not limited to decoction, granules, paste, tablets, capsules, oral liquid, powder or decoction piece.

[0019] For example, as an alternative, in the above use, the drug is in solid form, semi-solid form or liquid form.

[0020] For example, as an optional mode, in the above-mentioned use, the medicine is a decoction, a decoction, a powder, an oral liquid, an ointment, a pill, a granule, a tablet or a capsule.

[0021] Preferably, the drug has good safety, no liver and kidney toxicity, and can be taken for a long time.

[0022] It should be understood that within the scope of the present invention, the above-mentioned technical features of the present invention and the technical features specifically described below (such as embodiments) can be combined with each other to form a new or preferred technical solution. Due to space limitations, they will not be described one by one here.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] (1) The present invention has confirmed through a modern pharmacological model that the Chinese medicine composition of the present invention has a significant effect on the symptoms of blurred vision, dry eyes, eye swelling, and eye and orbital pain caused by visual fatigue. In particular, the Chinese medicine composition of the present invention can be used to relieve dry eye caused by visual fatigue.

[0025] (2) The Chinese medicine composition of the present invention has good safety, no liver and kidney toxicity, and can be taken for a long time. DETAILED DESCRIPTION

[0026] The efficacy and main treatment of each medicinal ingredient in the Chinese medicine composition of the present invention are as follows:

[0027] "Schisandra chinesis" is the mature fruit of Schisandra chinesis (Turcz.) Ball or Schisandra sphenanthera rehd.et Wils., a plant of the genus Schisandra in the family Schisandrae. It has the effects of astringing, strengthening qi and promoting body fluid, tonifying the kidney and calming the mind. It is mainly used to treat chronic cough, asthma, spontaneous sweating, night sweats, spermatorrhea, spermatorrhea, chronic diarrhea, thirst due to loss of body fluid, thirst, palpitations, insomnia, and dreaminess. Schisandra mainly contains volatile oils, organic acids, tannins, vitamins, sugars, and resins. The main component of the volatile oil in the seeds is schisandrin. Schisandra chinensis has an excitatory effect on the centers of the nervous system at all levels, and affects the excitation and inhibition processes of the cerebral cortex, making them tend to balance. It has an excitatory effect on the respiratory system, and has antitussive and expectorant effects. It can lower blood pressure. It can promote bile secretion, lower serum transaminase, and protect liver cells. It has an adaptogenic effect similar to ginseng, and can enhance the body's defense against nonspecific stimuli. It can increase cellular immune function and significantly enhance the SOD activity of the brain, liver, and spleen, so it has the effects of improving immunity, anti-oxidation, and anti-aging. It has an inhibitory effect on Staphylococcus aureus, Klebsiella pneumoniae, Salmonella enterica, Pseudomonas aeruginosa, etc.

[0028] "Cornus officinalis" is the dried mature pulp of Cornus officinalis Sieb.et Zucc., a plant of the genus Cornus in the family Cornaceae. It has the effects of nourishing the liver and kidneys, astringing and consolidating. It is mainly used to treat soreness of the waist and knees, dizziness and tinnitus, impotence, spermatorrhea, enuresis and frequent urination, metrorrhagia, menorrhagia, excessive sweating, and physical weakness. The fruit contains cornus glycoside, ursolic acid, moro-lonic acid, 7-O-methyl moro-lonic acid, swertia glycoside, and strychnoside. In addition, there are gallic acid, malic acid, tartaric acid, provitamin A, as well as saponins, tannins, etc. Cornus decoction can inhibit the growth of Staphylococcus aureus in vitro, the sour liquid obtained from the fresh pulp has an inhibitory effect on typhoid and dysentery bacilli, and the water extract has different degrees of inhibitory effect on Trichophyton violaceum in test tubes. Different doses of Cornus officinalis can significantly increase the content of serum IgG in mice. It also shows a significant inhibitory effect on contact dermatitis caused by DNCB. Cornus officinalis has a significant effect on counteracting adrenergic hyperglycemia and can increase liver glycogen. In addition, Cornus officinalis has certain diuretic, anti-inflammatory, anti-cancer, antioxidant, and anti-aging effects.

[0029] "Huangjing" is the dried rhizome of Polygonatum sibiricum Red., Polygonatum cyrtonema Hua or Polygonatum kingianum Coll.et Hemsl. of the genus Polygonatum in the Liliaceae family. It has the effects of nourishing yin and moistening the lungs, tonifying the spleen and replenishing qi, nourishing the kidneys and replenishing essence. It is mainly used to treat yin deficiency and fatigue cough, dry lung cough, spleen deficiency and fatigue, poor appetite and dry mouth, thirst; kidney deficiency and soreness of the waist and knees, impotence and spermatorrhea, tinnitus and dark eyes, premature graying of hair and beard, physical weakness and emaciation, wind leprosy and tinea. Huangjing contains mucus, starch, sugar, niacin and quinones. Polygonatum cyrtonema contains diaminobutyric acid, aspartic acid, homoserine, digitalis glycosides and various anthraquinone compounds. Huangjing has anti-pathogenic microorganisms, lowers blood lipids, delays aging and other effects, and has effects on the cardiovascular system, immune function, cyclic nucleotide content and blood sugar.

[0030] "Ligustrum lucidum fruit" is the mature fruit of Ligustrum lucidum Ait., a plant of the Oleaceae family. It has the effects of nourishing the liver and kidneys, improving eyesight and blackening hair. It is a yin-tonifying medicine under the category of tonic medicine. It is used to treat tinnitus, deafness, dizziness, soreness of the waist and knees, premature graying of hair and beard, etc. caused by liver and kidney yin deficiency. Modern pharmacological research shows that Ligustrum lucidum fruit and its peel contain oleanolic acid, ursolic acid, palmitic acid, stearic acid, linoleic acid, oleic acid, acetic oleanolic acid, etc. Ligustrum lucidum fruit contains a variety of chemical components, which have anti-cancer, anti-oxidation and aging, immune regulation, hypoglycemic and blood pressure lowering effects. In addition, it is also used to treat chronic atrophic gastritis, etc.

[0031] "Atractylodes" is the rhizome of Atractylodes macrocephala Koidz., a plant of the Asteraceae family. It has the effects of strengthening the spleen, replenishing qi, drying dampness and promoting diuresis, stopping sweating, and calming the fetus. It is a qi-tonifying medicine under the category of tonic medicine. It is used to treat weak spleen and stomach qi, loss of appetite, fatigue and lack of energy, bloating, diarrhea, phlegm and fluid, edema, jaundice, dampness and arthralgia, urinary difficulties, dizziness, spontaneous sweating, and fetal restlessness. Modern pharmacological research shows that the components of Atractylodes are volatile oils, acetic acid compounds, polysaccharides, glycosides, amino acids and other compounds. Atractylodes has high medicinal value, has more than 20 pharmacological effects such as promoting cellular immune function, significantly increasing serum IgG, improving the body's disease resistance, regulating gastrointestinal function, diuresis, anti-tumor, anti-inflammatory, antibacterial, anti-coagulation, and anti-aging.

[0032] "Mulberry bark" is the root bark of Morus alba L., a plant of the genus Morus in the Moraceae family. It has the effects of purging the lungs and relieving asthma, promoting diuresis and reducing swelling. It is mainly used to treat lung heat, cough and asthma, and edema. Mulberry bark contains a variety of flavonoid derivatives, such as morusin, morusin, morusin, etc.; umbelliferone, scopolamine, and also contains antihypertensive components with similar effects to acetylcholine; recently, mulberry furan A has been extracted. Mulberry bark has a mild cough-relieving effect and can act as a diuretic, increasing urine volume and the excretion of sodium, potassium, and chloride; the decoction and its ethanol, ether, and methanol extracts have different degrees of antihypertensive effects; it has sedative, stable, anticonvulsant, analgesic, and cooling effects on the nervous system; it has an excitatory effect on the intestines and uterus. The decoction has an inhibitory effect on Staphylococcus aureus, Salmonella typhi, and Shigella dysenteriae. Mulberry bark has an inhibitory effect on cervical cancer JTC28 and lung cancer cells. Recent studies have also shown that it can also resist HIV.

[0033] "Chrysanthemum" is the dried inflorescence of Chrysanthemum morifolium Ramat., a plant of the genus Chrysanthemum in the Asteraceae family. It has the effects of dispersing wind-heat, calming liver yang, clearing the liver and improving eyesight, clearing away heat and detoxifying. It is mainly used to treat wind-heat colds, the initial stage of febrile diseases, dizziness caused by liver yang, liver wind syndrome, red and blurred eyes, sores, carbuncle and swelling. Chrysanthemum contains volatile oils, including borneol, camphor, and chrysanthemum oil cycloketone. In addition, it also contains chrysanthemum glycosides, adenine, choline, flavonoids, stachydrine, vitamin A, vitamin B1, vitamin E, amino acids and acacia. Chrysanthemum water extract or decoction has a certain antibacterial effect on Staphylococcus aureus, various pathogenic bacilli and skin fungi. This product also has an inhibitory effect on influenza virus PR3 and Leptospira. Chrysanthemum preparations have the effects of dilating coronary arteries, increasing coronary blood flow, and increasing myocardial oxygen consumption, and have the effects of lowering blood pressure, shortening coagulation time, antipyretic, anti-inflammatory and sedative.

[0034] The present invention will be further described with reference to specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0035] If no specific techniques or conditions are specified in the examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0036] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples are commercially available products unless otherwise specified.

[0037] Preparation Example:

[0038] Embodiment 1:

[0039] A traditional Chinese medicine composition for relieving visual fatigue is prepared from the following raw materials in parts by weight: 7g of Schisandra chinensis, 7g of Cornus officinalis, 7g of Polygonatum sibiricum, 5g of Ligustrum lucidum, 5g of Atractylodes macrocephala, 2g of Morus alba bark, and 3g of Chrysanthemum.

[0040] The preparation method of the traditional Chinese medicine composition is as follows: for the first decoction, 500 mL of cold water is added to cover the medicine surface by about 3 cm, and the mixture is decocted after soaking for 30 minutes, firstly boiling over high heat, then decocting over low heat for 30-40 minutes, filtering the residue to obtain juice; for the second decoction, 500 mL of cold water is added to cover the medicine surface by about 2 cm, and the mixture is decocted for 20 minutes after the water boils, and the juice is obtained by filtering the residue, the two medicinal liquids are mixed, and 200 mL of concentrated liquid is obtained by vacuum concentration.

[0041] Embodiment 2:

[0042] A traditional Chinese medicine composition for relieving visual fatigue is prepared from the following raw materials in parts by weight: 9g of Schisandra chinensis, 7g of Cornus officinalis, 6g of Polygonatum sibiricum, 5g of Ligustrum lucidum, 5g of Atractylodes macrocephala, 2g of Morus alba bark, and 2g of Chrysanthemum.

[0043] The preparation method is the same as Example 1.

[0044] Embodiment 3:

[0045] A traditional Chinese medicine composition for relieving visual fatigue is prepared from the following raw materials in parts by weight: 6g of Schisandra chinensis, 7g of Cornus officinalis, 9g of Polygonatum sibiricum, 5g of Ligustrum lucidum, 5g of Atractylodes macrocephala, 2g of Morus alba bark, and 2g of Chrysanthemum.

[0046] The preparation method is the same as Example 1.

[0047] Comparative Example 1:

[0048] A traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 7g of wolfberry, 7g of cornus officinalis, 7g of polygonatum, 5g of glossy privet fruit, 5g of atractylodes macrocephala, 2g of mulberry bark, and 3g of chrysanthemum.

[0049] The preparation method is the same as Example 1.

[0050] Comparative Example 2:

[0051] A traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 9g of Schisandra chinensis, 7g of Cornus officinalis, 7g of Polygonatum sibiricum, 5g of Ligustrum lucidum, 5g of Atractylodes macrocephala, and 3g of Chrysanthemum.

[0052] The preparation method is the same as Example 1.

[0053] Comparative Example 3 (positive control drug, prepared according to the composition of Qiju Dihuang Pills):

[0054] A traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 7g of wolfberry, 3g of chrysanthemum, 7g of prepared rehmannia, 7g of cornus, 3g of peony bark, 3g of yam, 3g of poria, and 3g of oriental rhizome.

[0055] The preparation method is the same as Example 1.

[0056] Effect embodiment:

[0057] Effect Example 1: Effect of the Chinese medicine composition of the present invention on visual fatigue in rats caused by light 1. Experimental animals:

[0058] Clean grade male Sprague Dawley rats (weight 200-220g) were selected and adaptively fed for one week, and the experimental animals were fed and drank water normally. Then, the experimental animals were randomly divided into 8 groups, 8 in each group, namely normal control group, model control group, Example 1-3 groups, and Comparative Example 1-3 groups.

[0059] 2. Method of administration:

[0060] The normal control group and the model control group were gavaged with distilled water for one week. The Example 1-3 group and the Comparative Example 1-3 group were gavaged with the Chinese medicine composition prepared in the above "Preparation Example" for one week, and the dosage was 500 mg / kg / day.

[0061] 3. Lighting model construction:

[0062] The rats in the normal control group were not exposed to light. The other groups were exposed to light after the last administration. The light source was a 100W incandescent lamp at a vertical height of 30cm from the rats, and the illumination time was 20min. After the illumination ended, the state of the eyeballs of the rats in each group was observed under a slit lamp and recorded.

[0063] 4. Detection indicators:

[0064] The pupil diameter and eyelid opening value of rats in each group were measured, and the ratio of the two, i.e., pupil diameter / eyelid opening value, was calculated.

[0065] 5. Statistical analysis methods:

[0066] SPSS23.0 statistical software was used for data analysis, and the measurement data were expressed as mean ± standard deviation (mean ± SD). One-way ANOVA was used for comparison between groups, and the LSD test was used for equal variance test, and the Euskal-Walli SH method was used for non-parametric test for unequal variance. P < 0.05 was considered statistically significant, and P < 0.01 was considered extremely statistically significant.

[0067] 6. Experimental results:

[0068] The experimental results of pupil diameter / eyelid opening value of rats in each group are shown in Table 1.

[0069] Table 1: Effect of the Chinese medicine composition of the present invention on pupil diameter / eyelid opening value of rats

[0070] Group Pupil diameter / eyelid opening Normal control group 0.1852±0.0114 Model control group <![CDATA[0.2903±0.0547 ** ]]> Example 1 Group <![CDATA[0.1905±0.0228 ## ]]> Example 2 Group <![CDATA[0.2061±0.0239 ## ]]> Example 3 Group <![CDATA[0.2176±0.0391 # ]]> Comparative Example 1 0.2447±0.0413 Comparative Example 2 0.2529±0.0485 Comparative Example 3 (positive control) 0.2324±0.0532

[0071] Note: Compared with the normal control group, ** p<0.01, with extremely significant difference in statistical significance; compared with the model control group, # p<0.05, there is a statistically significant difference. ## p<0.01, which is a statistically significant difference.

[0072] As can be seen from the experimental results in Table 1, compared with the normal control group, the pupil diameter / eyelid opening value of the model control group rats was extremely significantly increased (P<0.01), indicating that the model was successfully prepared. The pupil diameter / eyelid opening value of the rats in the Example 1-3 group and the Comparative Example 1-3 group were reduced to varying degrees compared with the model control group rats. Among them, the experimental results of the Example 1-3 group were statistically significant compared with the model control group (P<0.05, P<0.01), and the effect of the Chinese medicine composition of the Example 1 group was the best. The experimental results of the Example 1-3 group were all better than the Comparative Example 3 (positive control) group to varying degrees.

[0073] In addition, it can be seen from the experimental results of comparative examples 1-2 that the type of raw materials used in the Chinese medicine composition of the present invention has a great influence on its efficacy. After removing some medicinal flavors from the Chinese medicine composition of the present invention or replacing them with medicinal flavors with similar efficacy, the overall efficacy of the Chinese medicine composition is significantly reduced.

[0074] Effect Example 2: Effect of the Chinese medicine composition of the present invention on dry eye caused by visual fatigue 1. Experimental animals:

[0075] Clean grade male healthy adult New Zealand white rabbits (weight 1.5-2.0 kg) were selected and adaptively fed for one week. The experimental animals were fed and drank water normally. Then, the experimental animals were randomly divided into 8 groups, 8 in each group. They were normal control group, model control group, Example 1-3 groups, and Comparative Example 1-3 groups.

[0076] 2. Construction of dry eye model:

[0077] The male rabbits were placed on a rabbit rack and anesthetized with ketamine intramuscularly. The skin was prepared, disinfected and draped. Then lidocaine was injected locally in the surgical area for local anesthesia. The scrotum was incised without testicle removal as the normal control group. The other 7 groups of male rabbits underwent bilateral orchiectomy, testicle and epididymis removal, vas deferens and spermatic vein ligation, scrotal skin continuous suture, and iodine was applied locally to prevent infection. Eight weeks after castration, the castrated male rabbit dry eye model was established.

[0078] 3. Method of administration:

[0079] After the ovariectomized male rabbit dry eye model was successfully established, the normal control group and the model control group were gavaged with distilled water for two weeks. The Example 1-3 group and the Comparative Example 1-3 group were gavaged with the Chinese medicine composition prepared in the above "Preparation Example" for two weeks, and the dosage was 10 g / kg / day.

[0080] 4. Detection indicators:

[0081] 4.1 Tear secretion volume (SIT) and tear breakup time (BUT):

[0082] At the end of the experiment, the tear secretion volume (SIT) and tear break-up time (BUT) were measured. The measurement method was to take a tear flow detection filter paper strip, fold it along the notch mark, and place the blunt end into the conjunctival sac of the middle and outer 1 / 3 of the rabbit's lower eyelid. The rabbit's eyes were naturally and completely closed. The length of the stained part was read after 5 minutes. If the length was <5mm, it indicated dry eyes. Tear break-up time detection: a glass rod dipped in fluorescein was dropped into the conjunctival sac of the rabbit's lower eyelid. The eyelids were closed to allow the fluorescein to be evenly distributed on the corneal surface. The upper and lower eyelids were then fixed to fully expose the cornea. The cornea was observed under a slit lamp with diamond blue light. The examination started from the last blink and the time when the first rupture point appeared on the conjunctiva was recorded.

[0083] 4.2 TNF-α and IL-6 levels in tears:

[0084] At the end of the experiment, 60 μL of 0.9% saline was instilled into the conjunctival sac of each group of male rabbits, and the lower eyelid conjunctiva was gently pulled and slightly pressed. The tears were aspirated with a capillary glass tube and transferred to a centrifuge tube and stored at -80°C. The levels of TNF-α and IL-6 in the tears were determined by enzyme-linked immunosorbent assay. OD was measured with an enzyme marker 450 value and calculate its corresponding concentration.

[0085] 5. Statistical analysis methods:

[0086] SPSS23.0 statistical software was used for data analysis, and the measurement data were expressed as mean ± standard deviation (mean ± SD). One-way ANOVA was used for comparison between groups, and the LSD test was used for equal variance test, and the Euskal-Walli SH method was used for non-parametric test for unequal variance. P < 0.05 was considered statistically significant, and P < 0.01 was considered extremely statistically significant.

[0087] 6. Experimental results:

[0088] 6.1 Tear secretion volume (SIT) and tear breakup time (BUT) of male rabbits in each group:

[0089] The experimental results of tear secretion volume (SIT) and tear breakup time (BUT) of male rabbits in each group are shown in Table 2.

[0090] Table 2: Effects of the Chinese medicine composition of the present invention on tear secretion (SIT) and tear breakup time (BUT) of male rabbits

[0091]

[0092]

[0093] Note: Compared with the normal control group, ** p<0.01, with extremely significant difference in statistical significance; compared with the model control group, # p<0.05, there is a statistically significant difference. ## p<0.01, which is a statistically significant difference.

[0094] As shown in the experimental results in Table 2, compared with the normal control group, the SIT and BUT of the male rabbits in the model control group were extremely significantly reduced (P<0.01), indicating that the model was successfully prepared. The SIT and BUT of the male rabbits in the embodiment 1-3 group and the comparative example 1-3 group were increased to varying degrees compared with the male rabbits in the model control group. Among them, the experimental results of the embodiment 1-3 group were statistically significant compared with the model control group (P<0.01), and the effect of the Chinese medicine composition in the embodiment 1 group was the best. The experimental results of the comparative example 1-2 group were also statistically significant compared with the model control group (P<0.05). The experimental results of the embodiment 1-3 group and the comparative example 1-2 group were better than the comparative example 3 (positive control) group, which shows that the Chinese medicine composition in the positive control group may only have the effect of relieving visual fatigue, but not the effect of treating dry eye.

[0095] In addition, it can be seen from the experimental results of comparative examples 1-2 that the type of raw materials used in the Chinese medicine composition of the present invention has a great influence on its efficacy. After removing some medicinal flavors from the Chinese medicine composition of the present invention or replacing them with medicinal flavors with similar efficacy, the overall efficacy of the Chinese medicine composition is significantly reduced.

[0096] 6.2 Levels of TNF-α and IL-6 in the tears of male rabbits in each group:

[0097] The experimental results of TNF-α and IL-6 levels in the tears of male rabbits in each group are shown in Table 3.

[0098] Table 3: Effects of the Chinese medicine composition of the present invention on the levels of TNF-α and IL-6 in male rabbit tears

[0099] Group TNF-α(ng / L) IL-6 (pg / L) Normal control group 90.38±5.94 81.76±4.08 Model control group <![CDATA[355.28±13.41 ** ]]> <![CDATA[218.65±9.57 ** ]]> Example 1 Group <![CDATA[194.75±10.62 ## ]]> <![CDATA[133.47±8.54 ## ]]> Example 2 Group <![CDATA[205.33±11.59 ## ]]> <![CDATA[152.91±9.03 ## ]]> Example 3 Group <![CDATA[218.43±2.64 ## ]]> <![CDATA[166.09±8.42 # ]]> Comparative Example 1 269.08±14.73 181.25±8.21 Comparative Example 2 276.19±11.16 187.82±8.31 Comparative Example 3 (positive control) 295.67±12.58 200.44±9.89

[0100] Note: Compared with the normal control group, ** p<0.01, with extremely significant difference in statistical significance; compared with the model control group, # p<0.05, there is a statistically significant difference. ## p<0.01, which is a statistically significant difference.

[0101] As shown in the experimental results in Table 3, compared with the normal control group, the TNF-α and IL-6 levels of male rabbits in the model control group were extremely significantly increased (P<0.01). The TNF-α and IL-6 levels of male rabbits in the embodiment 1-3 group and the comparative example 1-3 group were reduced to varying degrees compared with the male rabbits in the model control group. Among them, the experimental results of the embodiment 1-3 group were statistically significant compared with the model control group (P<0.01, P<0.05), and the effect of the Chinese medicine composition in the embodiment 1 group was the best. The experimental results of the embodiment 1-3 group and the comparative example 1-2 group were better than those of the comparative example 3 (positive control) group.

[0102] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A Chinese medicine composition for relieving visual fatigue, characterized in that: The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 1-20 parts of Schisandra chinensis, 1-20 parts of Cornus officinalis, 1-20 parts of Polygonatum sibiricum, 1-15 parts of Ligustrum lucidum, 1-15 parts of Atractylodes macrocephala, 1-10 parts of Morus alba bark, and 1-10 parts of Chrysanthemum.

2. The Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 1-15 parts of Schisandra chinensis, 1-15 parts of Cornus officinalis, 1-15 parts of Polygonatum sibiricum, 1-10 parts of Ligustrum lucidum, 1-10 parts of Atractylodes macrocephala, 1-8 parts of Morus alba bark, and 1-8 parts of Chrysanthemum.

3. The Chinese medicine composition according to claim 2, characterized in that: The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 5-10 parts of Schisandra chinensis, 5-10 parts of Cornus officinalis, 5-10 parts of Polygonatum sibiricum, 4-7 parts of Ligustrum lucidum, 4-7 parts of Atractylodes macrocephala, 2-5 parts of Morus alba bark, and 2-5 parts of Chrysanthemum.

4. The method for preparing the Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The preparation method comprises the following steps: Weigh each Chinese medicinal material in proportion by weight according to any one of claims 1 to 3, decoct for the first time, cover the medicinal surface with cold water, soak and then decoct, first boil over high heat, then decoct for a period of time over low heat, filter the residue and take the juice; decoct for the second time, cover the medicinal surface with cold water, decoct for a period of time after the water boils, filter the residue and take the juice, mix the two medicinal liquids and concentrate.

5. The preparation method according to claim 4, characterized in that: During the first decoction, the cold water should cover the surface of the medicine by about 3 cm. Soak for 30 minutes before decocting. Boil over high heat first, then simmer over low heat for 30-40 minutes. During the second decoction, the cold water should cover the surface of the medicine by about 2 cm. After the water boils, decoct for 20 minutes.

6. Use of the Chinese medicine composition according to any one of claims 1 to 3 or the Chinese medicine composition prepared by the preparation method according to claim 4 or claim 5 in the preparation of a medicament for relieving visual fatigue.

7. The use according to claim 6, characterized in that: The medicine is used to relieve the symptoms of blurred vision, dry eyes, swollen eyes, and sore eyes and eye sockets caused by visual fatigue.

8. The use according to claim 6, characterized in that: The medicine is used for relieving dry eyes caused by visual fatigue.

9. The use according to claim 6, characterized in that: The drug is in solid form, semi-solid form or liquid form.

10. The use according to claim 8, characterized in that: The medicine is in the form of decoction pieces, decoctions, powders, oral liquids, pastes, pills, granules, tablets or capsules.

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