Cell culture and monitoring integrated device

Abstract

The invention relates to the technical field of biomedicine, and discloses a cell culture and monitoring integrated device which comprises an overturning assembly and an overturning piece, the overturning assembly comprises an overturning frame, a clamping plate, a supporting column, an extrusion column and a rotating disc, the clamping plate is located in the overturning frame, the clamping plate is movably connected with the overturning frame, and the rotating disc is located in the overturning frame. The supporting column is fixed to one side of the clamping plate, the supporting column is connected to one side of the overturning frame in an inserted mode, the supporting column is movably connected with the overturning frame, the extrusion column is arranged on one side of the supporting column, and the rotating disc is fixed to one side of the overturning frame. The invention has the following beneficial effects: by arranging the turnover mechanism on the detector, the cell culture container can be turned over when the other side of the cell needs to be detected, so that the cell culture container can be turned over and then recovered to the original position and angle, thereby ensuring that the position of the cell in the visual field is almost unchanged when the two sides of the cell are observed; and data observed before and after can be accurately compared.

Description

Technical Field

[0001] The invention relates to the field of biomedical technology, in particular to a cell culture and monitoring integrated device. Background Art

[0002] When studying the basic growth characteristics of cells, such as the cell cycle and cell division rate, it is necessary to test the cells. Live cell imaging can continuously observe living cells for a long time and record the dynamic changes of cells in real time, such as cell growth, division, migration, movement and other behaviors, to provide direct visual evidence for studying the physiological processes of cells. Some special culture dishes or culture containers have transparent upper and lower bottoms, which can detect both sides of the cells. However, when testing both sides of the cells, it is necessary to manually touch the container to flip it. Manual flipping makes it difficult to ensure that the container returns to its original precise position and angle. In subsequent observations, this may cause the position of the cells in the field of view to change, making it difficult to accurately compare the data from previous and subsequent observations. Summary of the invention

[0003] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0004] In view of the above problems and / or the problems existing in the existing integrated cell culture and monitoring devices, the present invention is proposed.

[0005] Therefore, the problem to be solved by the present invention is that when testing both sides of cells, it is necessary to manually touch the container to flip it, and it is difficult to ensure that the container returns to its original precise position and angle by manual flipping.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a cell culture and monitoring integrated device, comprising a flip assembly, including a flip part, including a flip frame, a clamping plate, a support column, a squeezing column and a turntable, wherein the clamping plate is located in the flip frame, the clamping plate and the flip frame are movably connected, the support column is fixed to one side of the clamping plate, the support column is plugged into one side of the flip frame, the support column and the flip frame are movably connected, the squeezing column is arranged on one side of the support column, and the turntable is fixed to one side of the flip frame;

[0007] A bearing assembly is arranged on the flip frame, including a bearing member, including a bearing plate, a moving block, an extrusion block, an inclined block and a lower pressure strip, wherein the bearing plate is arranged at the bottom of the flip frame, the moving block is fixed to one side of the bearing plate, the extrusion block is arranged on one side of the moving block, the inclined block is fixed to one side of the extrusion block, and the lower pressure strip is arranged on the top of the inclined block.

[0008] As a preferred solution of the integrated cell culture and monitoring device of the present invention, the flip assembly further comprises a moving part, which is arranged on the clamping plate, and a connecting block is fixed to one side of the clamping plate.

[0009] As a preferred solution of the integrated cell culture and monitoring device described in the present invention, a rack is fixed on one side of the connecting block, a gear is provided on one side of the rack, the rack and the gear are meshed, and the gear is rotatably connected to the inner wall of the flip frame through a rotating shaft.

[0010] As a preferred solution of the integrated cell culture and monitoring device of the present invention, a slider is fixed to one side of the clamping plate, a first spring is fixed to one side of the slider, and the slider slides in the flip frame.

[0011] As a preferred solution of the integrated cell culture and monitoring device of the present invention, a support bar is fixed to one side of the slider, a support block is fixed to one end of the first spring, and the support block is fixed to the inner wall of the flip frame.

[0012] As a preferred solution of the integrated cell culture and monitoring device of the present invention, the bearing assembly further comprises a limiter disposed on the bearing plate, and a second spring is fixed to one side of the bearing plate.

[0013] As a preferred solution of the integrated cell culture and monitoring device of the present invention, a hinged rod is provided on one side of the lower pressing bar, a limit block is provided at one end of the hinged rod, and the hinged rod is hinged to the lower pressing bar and the limit block.

[0014] As a preferred solution of the integrated cell culture and monitoring device described in the present invention, a positioning plate is provided on one side of the hinged rod, the positioning plate and the hinged rod are hinged, a torsion spring is fixed on one side of the positioning plate, the torsion spring is fixed to one side of the hinged rod, a support frame is sleeved on the outer side of the lower pressure strip, and the support frame and the lower pressure strip are movably connected.

[0015] As a preferred solution of the integrated cell culture and monitoring device described in the present invention, it also includes a main body component, which is arranged on the flip frame, including a detection table and a detection instrument, the detection table is sleeved on the outside of the support column, the detection table and the support column are rotatably connected by a rotating shaft, and the detection instrument is fixed on one side of the detection table.

[0016] As a preferred solution of the integrated cell culture and monitoring device of the present invention, a base is fixed at the bottom of the detection platform.

[0017] The beneficial effect of the present invention is that by arranging a flipping mechanism on the detector, it can be flipped when the other side of the cell needs to be detected, so that the cell culture container can be restored to its original position and angle after flipping, which ensures that when observing both sides of the cell, the position of the cell in the field of view hardly changes, so that the data of the previous and next observations can be accurately compared. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0019] Figure 1 This is the overall structural diagram of the integrated cell culture and monitoring device.

[0020] Figure 2 This is a cross-sectional structural diagram of the detection platform of the integrated cell culture and monitoring device.

[0021] Figure 3 An integrated device for cell culture and monitoring Figure 2 A partial enlarged structural diagram in the middle.

[0022] Figure 4 This is a structural diagram of the mobile blocks of the integrated cell culture and monitoring device.

[0023] Figure 5 This is a diagram of the rack structure of the integrated cell culture and monitoring device.

[0024] Figure 6 An integrated device for cell culture and monitoring Figure 5 A partial enlarged structural diagram of point B in the middle. DETAILED DESCRIPTION

[0025] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.

[0026] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0027] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0028] Example 1

[0029] Reference Figure 2 to Figure 6 , which is the first embodiment of the present invention, provides an integrated cell culture and monitoring device, which includes a flipping component 200, a supporting component 300 and a main body component 100. The three components cooperate to flip the cell culture container and restore it to its original position and angle for detection.

[0030] The flip assembly 200 includes a flip part 201, including a flip frame 201a, a clamping plate 201b, a support column 201c, an extrusion column 201d and a turntable 201e. The clamping plate 201b is located in the flip frame 201a, the clamping plate 201b and the flip frame 201a are movably connected, the support column 201c is fixed to one side of the clamping plate 201b, the support column 201c is plugged into one side of the flip frame 201a, the support column 201c and the flip frame 201a are movably connected, the extrusion column 201d is set on one side of the support column 201c, and the turntable 201e is fixed to one side of the flip frame 201a.

[0031] There are two clamping plates 201b, both of which are located in the flip frame 201a. The squeezing column 201d is fixed to the inner wall of the detection platform 101. One end of the support column 201c is set as an inclined surface, and the squeezing column 201d is set as an inclined surface. After the cell container is placed on the detection platform 101 for detection, it is necessary to detect the other side of the cell. By rotating the turntable 201e, the rotation of the turntable 201e will drive the flip frame 201a to rotate. When the flip frame 201a rotates, it will drive the support column 201c to rotate. At this time, the inclined surface on the squeezing column 201d will squeeze the support column 201c to move the support column 201c. The movement of the support column 201c can drive the clamping plate 201b to move. At this time, the two clamping plates 201b can be moved toward each other, so that the cell container can be clamped, and then it can be turned over. After the turning is completed, the inclined surfaces of the support column 201c and the squeezing column 201d will be engaged, so that the clamping of the cell container can be released.

[0032] The supporting assembly 300 is arranged on the flip frame 201a, and includes a supporting member 301, including a supporting plate 301a, a moving block 301b, an extrusion block 301c, an inclined block 301d and a lower pressure strip 301e. The supporting plate 301a is arranged at the bottom of the flip frame 201a, the moving block 301b is fixed to one side of the supporting plate 301a, the extrusion block 301c is arranged at one side of the moving block 301b, the inclined block 301d is fixed to one side of the extrusion block 301c, and the lower pressure strip 301e is arranged at the top of the inclined block 301d.

[0033] There are two carrying plates 301a, both of which are located in the detection platform 101. The carrying plates 301a are movably connected to the detection platform 101. When the cells are detected, the cell container can be supported by the setting of the carrying plates 301a to prevent the cell container from shifting. When the flip frame 201a needs to be flipped, the limit of the turntable 201e needs to be released first. When the limit of the turntable 201e is released, the lower pressing bar 301e needs to be pressed down. The lower pressing bar 301e squeezes the inclined surface block 301d. The inclined surface block 301d moves to squeeze the squeezing block 301c. The squeezing block 301c moves It can drive the moving block 301b to move. There are two moving blocks 301b, both of which are fixed on one side of the carrying plate 301a. The movement of the moving block 301b will drive the carrying plate 301a to move. At this time, the carrying plate 301a can be moved into the detection table 101. A friction block is provided on one side of the clamping plate 201b, and one side of the container is engaged with the friction block. At this time, the container will not fall. Then, the flip frame 201a is flipped to drive the clamping plate 201b to move and clamp the container tightly so that it can be flipped. After the flipping is completed, the turntable 201e is limited, so that the carrying plate 301a can return to its original position.

[0034] Example 2

[0035] Reference Figure 2 to Figure 6 , which is the second embodiment of the present invention, and this embodiment is based on the previous embodiment.

[0036] Specifically, the flip assembly 200 further includes a moving member 202 disposed on a clamping plate 201 b , and a connecting block 202 a is fixed to one side of the clamping plate 201 b .

[0037] There are two connecting blocks 202a, both fixed on one side of the clamping plate 201b. The connecting blocks 202a slide inside the flip frame 201a. When the clamping plate 201b moves, the connecting blocks 202a can be driven to move. The movement of the connecting blocks 202a can support the clamping plate 201b to prevent the clamping plate 201b from shifting.

[0038] Specifically, a rack 202b is fixed to one side of the connecting block 202a, a gear 202c is provided to one side of the rack 202b, the rack 202b and the gear 202c are meshed, and the gear 202c is rotatably connected to the inner wall of the flip frame 201a through a rotating shaft.

[0039] There are two racks 202b, both fixed on one side of the connecting block 202a. When the connecting block 202a moves, the rack 202b can be driven to move. The movement of the rack 202b will drive the gear 202c to rotate. The rotation of the gear 202c drives the other rack 202b to move. The movement of the rack 202b will drive the other clamping plate 201b to move, so that the clamping plates 201b can move toward each other.

[0040] Specifically, a slider 202d is fixed to one side of the clamping plate 201b, a first spring 202e is fixed to one side of the slider 202d, and the slider 202d slides inside the flip frame 201a.

[0041] There are two sliders 202d, both of which slide in the flip frame 201a. There are two first springs 202e, both of which are fixed to the slider 202d for movement. When the slider 202d moves, a squeezing force can be applied to the first spring 202e, and the rebound force of the first spring 202e can drive the slider 202d to return to its original position.

[0042] Specifically, a support bar 202f is fixed to one side of the sliding block 202d, a support block 202g is fixed to one end of the first spring 202e, and the support block 202g is fixed to the inner wall of the flip frame 201a.

[0043] There are two support bars 202f, both located inside the first spring 202e. The support bars 202f can support the first spring 202e to prevent the first spring 202e from shifting when the first spring 202e is squeezed. The support block 202g is used to support the first spring 202e to prevent the first spring 202e from shifting.

[0044] Specifically, the bearing assembly 300 further includes a limiting member 302 disposed on the bearing plate 301 a , and a second spring 302 a is fixed to one side of the bearing plate 301 a .

[0045] There are four second springs 302a, all fixed to the inner wall of the testing platform 101. When the supporting plate 301a moves, a squeezing force can be applied to the second springs 302a, and the rebound force of the second springs 302a can drive the supporting plate 301a to return to its original position.

[0046] Example 3

[0047] Reference Figure 1 to Figure 6 , which is the third embodiment of the present invention, and is based on the first two embodiments.

[0048] Specifically, a hinged rod 302b is provided at one side of the lower pressing strip 301e, a limiting block 302c is provided at one end of the hinged rod 302b, and the hinged rod 302b is hinged to the lower pressing strip 301e and the limiting block 302c.

[0049] A limiting groove corresponding to the limiting block 302c is provided on the turntable 201e. The turntable 201e can be limited by engaging the limiting groove with the limiting block 302c. When the turntable 201e needs to be limited, the lower pressure bar 301e is pressed. At this time, the lower pressure bar 301e will drive the hinge rod 302b to move. The movement of the hinge rod 302b will drive the limiting groove and the limiting block 302c to separate, thereby releasing the limit on the turntable 201e.

[0050] Specifically, a positioning plate 302d is provided on one side of the hinged rod 302b, the positioning plate 302d and the hinged rod 302b are hinged, a torsion spring 302e is fixed on one side of the positioning plate 302d, the torsion spring 302e is fixed to one side of the hinged rod 302b, a support frame 302f is sleeved on the outer side of the lower pressure strip 301e, and the support frame 302f and the lower pressure strip 301e are movably connected.

[0051] There are two positioning plates 302d, both of which are fixed on the top of the testing platform 101. The support frame 302f is fixed on the top of the testing platform 101. When the hinged rod 302b is moved, a torsional force can be applied to the torsion spring 302e. When the hinged rod 302b is released, the rotational force of the torsion spring 302e can drive the hinged rod 302b to return to its original position. The positioning plate 302d is used to support the hinged rod 302b to prevent the hinged rod 302b from shifting. The support frame 302f is used to support the lower pressure strip 301e to prevent the lower pressure strip 301e from shifting.

[0052] Specifically, it also includes a main body component 100, which is arranged on the flip frame 201a, including a detection table 101 and a detection instrument 102. The detection table 101 is sleeved on the outside of the support column 201c, and the detection table 101 and the support column 201c are rotatably connected through a rotating shaft. The detection instrument 102 is fixed to one side of the detection table 101.

[0053] The detection table 101 is a platform for placing cell samples. The detection table 101 provides a stable support surface for the cell culture container to ensure that the cell sample can maintain a fixed position during the imaging process. The detector 102 can convert information such as cell morphology, structure, and activity into image signals and transmit the image data to a computer or other data storage device for subsequent analysis and research.

[0054] Specifically, a base 103 is fixed at the bottom of the testing platform 101 .

[0055] The base 103 is the basic supporting structure of the testing platform 101 , and provides stable support for the entire testing platform 101 .

[0056] When in use, after the cell container is placed on the testing table 101 for testing, it is necessary to test the other side of the cell. First, the limit of the turntable 201e needs to be released by pressing the lower pressure bar 301e. The lower pressure bar 301e can drive the hinge rod 302b to move when it moves. The hinge rod 302b can apply a torsional force to the torsion spring 302e when it moves. The hinge rod 302b moves to drive the limit block 302c to separate from the turntable 201e, so that the limit of the turntable 201e can be released. When the pressure strip 301e is pressed, the inclined surface block 301d is squeezed at the same time. The inclined surface block 301d moves to squeeze the squeezing block 301c. The movement of the squeezing block 301c can drive the moving block 301b to move. The movement of the moving block 301b can drive the carrying plate 301a to move. At this time, the carrying plate 301a can be moved into the testing platform 101. A friction block is provided on one side of the clamping plate 201b. One side of the container is engaged with the friction block. At this time, the container will not fall, and then the flip frame 201a can be flipped.

[0057] By rotating the turntable 201e, the rotation of the turntable 201e will drive the flip frame 201a to rotate, and when the flip frame 201a rotates, it will drive the support column 201c to rotate. At this time, the inclined surface on the squeezing column 201d will squeeze the support column 201c to move the support column 201c, and the movement of the support column 201c can drive the clamping plate 201b to move. At this time, the two clamping plates 201b can move toward each other, so that the cell container can be clamped, and then it can be flipped. After the flipping is completed, the limit of the turntable 201e is released, and the supporting plate 301a will return to its original position to support the container.

[0058] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. An integrated cell culture and monitoring device, characterized in that: include, A flip assembly (200) comprises a flip part (201), comprising a flip frame (201a), a clamping plate (201b), a support column (201c), a pressing column (201d) and a turntable (201e), wherein the clamping plate (201b) is located in the flip frame (201a), the clamping plate (201b) and the flip frame (201a) are movably connected, the support column (201c) is fixed to one side of the clamping plate (201b), the support column (201c) is plugged into one side of the flip frame (201a), the support column (201c) and the flip frame (201a) are movably connected, the pressing column (201d) is arranged on one side of the support column (201c), and the turntable (201e) is fixed to one side of the flip frame (201a); The bearing assembly (300) is arranged on the flip frame (201a), comprising a bearing member (301), comprising a bearing plate (301a), a moving block (301b), an extrusion block (301c), an inclined block (301d) and a lower pressing strip (301e), wherein the bearing plate (301a) is arranged at the bottom of the flip frame (201a), the moving block (301b) is fixed to one side of the bearing plate (301a), the extrusion block (301c) is arranged at one side of the moving block (301b), the inclined block (301d) is fixed to one side of the extrusion block (301c), and the lower pressing strip (301e) is arranged at the top of the inclined block (301d).

2. The integrated cell culture and monitoring device according to claim 1, characterized in that: The turnover assembly (200) further comprises a moving part (202) which is arranged on the clamping plate (201b), and a connecting block (202a) is fixed on one side of the clamping plate (201b).

3. The integrated cell culture and monitoring device according to claim 2, characterized in that: A rack (202b) is fixed on one side of the connection block (202a), a gear (202c) is arranged on one side of the rack (202b), the rack (202b) and the gear (202c) are meshed, and the gear (202c) is rotatably connected to the inner wall of the flip frame (201a) via a rotating shaft.

4. The integrated cell culture and monitoring device according to claim 3, characterized in that: A slider (202d) is fixed on one side of the clamping plate (201b), a first spring (202e) is fixed on one side of the slider (202d), and the slider (202d) slides inside the flip frame (201a).

5. The integrated cell culture and monitoring device according to claim 4, characterized in that: A support bar (202f) is fixed to one side of the sliding block (202d), a support block (202g) is fixed to one end of the first spring (202e), and the support block (202g) is fixed to the inner wall of the flip frame (201a).

6. The integrated cell culture and monitoring device according to claim 4 or 5, characterized in that: The bearing assembly (300) further comprises a limiting member (302) which is arranged on the bearing plate (301a), and a second spring (302a) is fixed to one side of the bearing plate (301a).

7. The integrated cell culture and monitoring device according to claim 6, characterized in that: A hinged rod (302b) is provided on one side of the lower pressing strip (301e), a limit block (302c) is provided on one end of the hinged rod (302b), and the hinged rod (302b) is hingedly connected to the lower pressing strip (301e) and the limit block (302c).

8. The integrated cell culture and monitoring device according to claim 7, characterized in that: A positioning plate (302d) is provided on one side of the hinge rod (302b), the positioning plate (302d) and the hinge rod (302b) are hinged, a torsion spring (302e) is fixed on one side of the positioning plate (302d), the torsion spring (302e) is fixed to one side of the hinge rod (302b), a support frame (302f) is sleeved on the outer side of the lower pressure strip (301e), and the support frame (302f) and the lower pressure strip (301e) are movably connected.

9. The integrated cell culture and monitoring device according to claim 7 or 8, characterized in that: It also comprises a main body assembly (100), which is arranged on the flip frame (201a), and comprises a detection platform (101) and a detection instrument (102); the detection platform (101) is sleeved on the outside of the support column (201c); the detection platform (101) and the support column (201c) are rotatably connected via a rotating shaft; and the detection instrument (102) is fixed to one side of the detection platform (101).

10. The integrated cell culture and monitoring device according to claim 9, characterized in that: A base (103) is fixed at the bottom of the detection platform (101).

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