Bacterial liquid concentration detection device and method based on multi-angle rotation spectrum

Abstract

The invention discloses a bacterial liquid concentration detection device and method based on a multi-angle rotation spectrum, and relates to the technical field of bacterial liquid concentration detection.The device comprises a light source, a transmitting optical fiber terminal, a centrifugal tube, a receiving optical fiber terminal, a spectrograph, a rotating platform and a computer; the rotating platform is used for driving the transmitting optical fiber terminal to rotate; the computer extracts multi-angle rotation spectrum fusion features according to original spectrum data, and the original spectrum data comprises spectrum data in different wave band ranges at different transmitting optical fiber terminal positions; the multi-angle rotation spectrum fusion features serve as input, the concentration of the sample solution to be detected is obtained through a concentration detection model, and the purpose of nondestructive detection of the concentration of the coprophilous bacteria solution is achieved. By acquiring spectral data of different wave band ranges at different transmitting optical fiber terminal positions, spectral characteristic information related to activity in the coprophilous fungus liquid can be effectively improved, so that the concentration of the coprophilous fungus liquid is accurately detected.

Description

Technical Field

[0001] The present application relates to the technical field of bacterial liquid concentration detection, and in particular to a bacterial liquid concentration detection device and method based on multi-angle rotation spectrum. Background Art

[0002] At present, there are various technical means for detecting solution concentration, including turbidimetry, plate culture counting method, flow cytometer detection method, ATP bioluminescence method and electrochemical sensing method. However, each method has its limitations. Turbidimetry is easily interfered by impurities in the sample, resulting in inaccurate measurement results; the plate culture counting method is cumbersome and time-consuming, and is only applicable to microorganisms grown on solid culture media; flow cytometer detection method requires expensive equipment and complex operation procedures, and samples also need to be specially stained and processed; ATP bioluminescence method requires special luminescence detection equipment, and the sample preparation process is complicated; the sensor of electrochemical sensing method is easily contaminated by impurities in the sample.

[0003] To address the above issues, we can use the rapid, non-contact, and non-destructive detection characteristics of spectral technology to conduct low-cost rapid and non-destructive testing of sample concentrations. This does not require additional sample processing, is simple to operate, and does not contaminate the sample.

[0004] However, traditional spectral measurement methods usually only use light of a single wavelength. In practical applications, due to the complex properties of fecal fungus liquid and numerous interference factors, traditional spectral methods are often unable to effectively detect the concentration information of fecal fungus liquid, affecting the detection accuracy of fecal fungus liquid concentration. Summary of the invention

[0005] The purpose of this application is to provide a bacterial liquid concentration detection device and method based on multi-angle rotation spectrum, which can effectively detect the concentration information of fecal bacteria liquid and improve the detection accuracy of fecal bacteria liquid concentration.

[0006] To achieve the above objectives, this application provides the following solutions:

[0007] In a first aspect, the present application provides a bacterial liquid concentration detection device based on multi-angle rotation spectroscopy, comprising:

[0008] Light source, transmitting optical fiber terminal, centrifuge tube, receiving optical fiber terminal, spectrometer, rotating platform and computer;

[0009] The light source is connected to the transmitting optical fiber terminal via an optical fiber;

[0010] The receiving optical fiber terminal is connected to the spectrometer via an optical fiber;

[0011] The spectrometer is connected to the computer;

[0012] The light source is used to provide photons in different wavelength bands;

[0013] The centrifuge tube is used to hold the sample solution to be tested;

[0014] The transmitting optical fiber terminal is used to irradiate photons to the sample solution to be tested;

[0015] The receiving optical fiber terminal is used to receive photons transmitted from the sample solution to be tested;

[0016] The transmitting optical fiber terminal and the centrifuge tube are both located on the rotating platform;

[0017] The rotating platform is used to drive the transmitting optical fiber terminal to rotate;

[0018] The spectrometer is used to obtain original spectral data according to the photons transmitted by the sample solution to be tested, wherein the original spectral data includes spectral data of different wavelength ranges at different emission optical fiber terminal positions;

[0019] The computer is used to extract multi-angle rotation spectrum fusion features according to the original spectrum data; and use the multi-angle rotation spectrum fusion features as input to obtain the concentration of the sample solution to be tested using a concentration detection model.

[0020] In a second aspect, the present application provides a method for detecting bacterial liquid concentration based on multi-angle rotation spectroscopy, wherein the method for detecting bacterial liquid concentration based on multi-angle rotation spectroscopy is implemented using the bacterial liquid concentration detection device based on multi-angle rotation spectroscopy described in the first aspect, and the method for detecting bacterial liquid concentration based on multi-angle rotation spectroscopy includes:

[0021] Acquire original spectral data of the sample solution to be tested, wherein the original spectral data includes spectral data of different wavelength bands at different transmitting optical fiber terminal positions;

[0022] Extracting multi-angle rotation spectrum fusion features according to the original spectrum data;

[0023] The multi-angle rotation spectrum fusion feature is used as input, and the concentration of the sample solution to be tested is obtained using a concentration detection model.

[0024] According to the specific embodiments provided in this application, this application discloses the following technical effects:

[0025] The present application provides a device and method for detecting the concentration of bacterial liquid based on multi-angle rotation spectrum, the device comprising: a light source, a transmitting optical fiber terminal, a centrifuge tube, a receiving optical fiber terminal, a spectrometer, a rotating platform and a computer; the rotating platform is used to drive the transmitting optical fiber terminal to rotate; the computer extracts the multi-angle rotation spectrum fusion feature according to the original spectrum data, wherein the original spectrum data includes spectrum data of different wavelength ranges at different transmitting optical fiber terminal positions; the multi-angle rotation spectrum fusion feature is used as input, and the concentration of the sample solution to be tested is obtained using the concentration detection model, so as to achieve the purpose of non-destructive detection of fecal bacteria liquid concentration. By obtaining spectrum data of different wavelength ranges at different transmitting optical fiber terminal positions, the spectral feature information about the activity in the fecal bacteria liquid can be effectively improved, so as to accurately detect the concentration of the fecal bacteria liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0027] FIG1( a ) is a front view of a bacterial liquid concentration detection device based on multi-angle rotation spectroscopy in Example 1 of the present application;

[0028] FIG1( b ) is a top view of a bacterial liquid concentration detection device based on multi-angle rotation spectrum in Example 1 of the present application;

[0029] Figure 2 This is a schematic diagram of a process for detecting bacterial liquid concentration based on multi-angle rotation spectroscopy in Example 2 of the present application;

[0030] Figure 3 Schematic diagram of the technical concept of a bacterial liquid concentration detection method based on multi-angle rotation spectroscopy in Example 2 of the present application.

[0031] Reference numerals:

[0032] 1-light source; 2-transmitting optical fiber; 3-transmitting optical fiber terminal; 4-transmitting optical fiber terminal bracket; 5-centrifuge tube; 6-centrifuge tube bracket; 7-receiving optical fiber terminal; 8-receiving optical fiber terminal bracket; 9-one-to-two optical fiber; 10-visible light spectrometer; 11-near-infrared light spectrometer; 12-rotating platform; 13-base. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0034] The study found that traditional spectral methods often only use one band of light and measure at a fixed angle, which is easily interfered by multiple factors. At the same time, it was found that in actual applications, the properties of fecal bacteria liquid are complex and the interference factors are huge. Traditional spectral methods still require sampling and dilution operations to detect effective concentration information.

[0035] Based on this, this embodiment provides a bacterial liquid concentration detection device and method based on multi-angle rotation spectroscopy. By utilizing a rotating platform and using light in different wavelength ranges to perform measurements at any angle, the concentration of fecal bacteria liquid is predicted in combination with corresponding data processing methods. This can effectively improve the spectral characteristic information related to the activity in the bacterial liquid, and provide a new method for fecal bacteria liquid concentration detection in practical applications.

[0036] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the present application is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0037] Example 1

[0038] This embodiment provides a bacterial liquid concentration detection device based on multi-angle rotation spectrum, including:

[0039] Light source, transmitting optical fiber terminal, transmitting optical fiber terminal bracket, centrifuge tube, centrifuge tube bracket, receiving optical fiber terminal, receiving optical fiber terminal bracket, rotating platform, spectrometer, computer and base.

[0040] The light source is connected to the transmitting optical fiber terminal through an optical fiber.

[0041] The receiving optical fiber terminal is connected to the spectrometer via an optical fiber.

[0042] The spectrometer is connected to the computer.

[0043] The centrifuge tube is used to hold the sample solution to be tested.

[0044] The centrifuge tube support is used to fix the centrifuge tube.

[0045] The emitting optical fiber terminal is used to irradiate photons to the sample solution to be tested.

[0046] The receiving optical fiber terminal is used to receive photons transmitted from the sample solution to be tested.

[0047] The transmitting optical fiber terminal bracket is used to fix the transmitting optical fiber terminal.

[0048] The receiving optical fiber terminal bracket is used to fix the receiving optical fiber terminal.

[0049] The rotating platform is used to drive the transmitting optical fiber terminal to rotate.

[0050] The centrifuge tube support and the transmitting optical fiber terminal support are both located on the rotating platform.

[0051] The spectrometer is used to obtain original spectral data according to the photons transmitted by the sample solution to be tested, wherein the original spectral data includes spectral data of different wavelength ranges at different emission optical fiber terminal positions, and the spectrometer includes a visible light spectrometer and a near-infrared light spectrometer; the visible light spectrometer and the near-infrared light spectrometer are respectively connected to the computer; the visible light spectrometer and the near-infrared light spectrometer are respectively connected to the receiving optical fiber terminal through optical fibers; the visible light spectrometer is used to obtain visible light spectral data at different emission optical fiber terminal positions according to the visible light photons transmitted by the sample solution to be tested; the near-infrared light spectrometer is used to obtain near-infrared light spectral data at different emission optical fiber terminal positions according to the near-infrared photons transmitted by the sample solution to be tested.

[0052] The computer is used to extract multi-angle rotation spectrum fusion features according to the original spectrum data; and use the multi-angle rotation spectrum fusion features as input to obtain the concentration of the sample solution to be tested using a concentration detection model.

[0053] The rotating platform and the receiving optical fiber terminal bracket are both fixed on the base.

[0054] As an example, as shown in Figure 1(a) and Figure 1(b), the nondestructive detection device for bacterial liquid activity based on multi-angle rotation spectrum in this embodiment includes a light source 1, a transmitting optical fiber 2, a transmitting optical fiber terminal 3, a transmitting optical fiber terminal bracket 4, a centrifuge tube 5, a centrifuge tube bracket 6, a receiving optical fiber terminal 7, a receiving optical fiber terminal bracket 8, a one-to-two optical fiber 9, a visible light spectrometer 10, a near-infrared spectrometer 11, a rotating platform 12 and a base 13.

[0055] The light source 1 is used to provide incident light with a wavelength ranging from 360nm to 2400nm.

[0056] The light source 1 is directly connected to the transmitting optical fiber 2.

[0057] The transmitting optical fiber 2 is used to transmit the incident light provided by the light source 1 to the transmitting optical fiber terminal 3 .

[0058] The transmitting optical fiber 2 is directly connected to the transmitting optical fiber terminal 3.

[0059] The transmitting optical fiber terminal 3 is used to transmit photons to irradiate the sample (ie, the sample solution to be tested).

[0060] The transmitting optical fiber terminal 3 is fixed on the transmitting optical fiber terminal bracket 4 .

[0061] The transmitting optical fiber terminal bracket 4 is fixed on the rotating platform 12 and can rotate with the rotating platform 12. The rotatable angle is 0° to 360°. The angle direction and range used in the analysis of this embodiment is 0° to 90° clockwise.

[0062] The centrifuge tube 5 is used to store the sample solution to be tested.

[0063] The centrifuge tube 5 is placed on the centrifuge tube holder 6 .

[0064] The receiving optical fiber terminal 7 is used to receive the photons that pass through the sample and transmit them to the one-to-two optical fiber 9 .

[0065] The receiving optical fiber terminal 7 is fixed on the receiving optical fiber terminal bracket 8 .

[0066] The receiving optical fiber terminal 7 is directly connected to the one-to-two optical fiber 9 .

[0067] The one-to-two optical fiber 9 is used to split the photons received in the receiving optical fiber terminal 7 into two parts, and transmit them to the visible light spectrometer 10 and the near infrared spectrometer 11 respectively.

[0068] The one-to-two optical fiber 9 is directly connected to the visible light spectrometer 10 and the near infrared spectrometer 11 respectively.

[0069] The visible light spectrometer 10 is used to analyze the light in the visible light band in the one-to-two optical fiber 9 and transmit the analysis data to the computer.

[0070] The visible light spectrometer 10 is directly connected to the computer.

[0071] The near infrared spectrometer 11 is used to analyze the light in the near infrared band in the one-to-two optical fiber 9 and transmit the analysis data to the computer.

[0072] The near infrared spectrometer 11 is directly connected to the computer.

[0073] The rotating platform 12 can rotate 0° to 360°. For the convenience of description, position a in FIG1(b) is defined as 0°. Position a rotates 90° clockwise to position b. When performing rotational spectral analysis, only the angle between position a and position b is used for analysis.

[0074] The rotating platform 12 drives the transmitting optical fiber terminal bracket 4 to rotate, thereby changing the angle formed by the transmitting optical fiber terminal 3 and the receiving optical fiber terminal 7, thereby achieving the purpose of changing the angle of the incident light.

[0075] The base 13 is used to fix or support various brackets and equipment. The centrifuge tube bracket 6 , the receiving optical fiber terminal bracket 8 and the rotating platform 12 are all fixed on the base 13 .

[0076] The bacterial liquid concentration detection device based on multi-angle rotation spectrum provided in this embodiment can effectively enhance the spectral characteristic information related to the activity in the fecal bacteria liquid by acquiring spectral data in different bands at different transmitting optical fiber terminal positions, thereby accurately detecting the concentration of the fecal bacteria liquid.

[0077] Example 2

[0078] like Figure 2 As shown, this embodiment provides a method for detecting bacterial liquid concentration based on multi-angle rotation spectroscopy, which is implemented using the bacterial liquid activity nondestructive detection device based on multi-angle rotation spectroscopy described in Example 1. The bacterial liquid activity nondestructive detection method based on multi-angle rotation spectroscopy includes:

[0079] S1: Acquire original spectral data of the sample solution to be tested, wherein the original spectral data includes spectral data of different wavelength bands at different emission optical fiber terminal positions.

[0080] S2: Extracting multi-angle rotation spectrum fusion features according to the original spectrum data.

[0081] S2 specifically includes:

[0082] S2-1: Obtain an absorbance curve data group based on the original spectral data, wherein the absorbance curve data group includes absorbance curves at several different transmitting optical fiber terminal positions, and each absorbance curve refers to a curve determined according to the absorbance of photons in different wavelength ranges at the same transmitting optical fiber terminal position.

[0083] S2-2: Determine a first eigenvector according to the absorbance curve data set.

[0084] S2-3: Calculate the area of ​​the target graph, wherein the target graph refers to a graph enclosed by each of the absorbance curves and the coordinate axis.

[0085] S2-4: Determine a second eigenvector according to the area.

[0086] S2-5: Determine the peak point in each absorbance curve.

[0087] S2-6: Determine a third eigenvector according to the peak point.

[0088] S2-7: Determine the fourth eigenvector based on the first absorbance and the second absorbance, wherein the first absorbance refers to the absorbance of photons in different wavelength ranges at different transmitting optical fiber terminal positions, and the second absorbance refers to the absorbance of photons in different wavelength ranges at the initial position, and the initial position refers to the transmitting optical fiber terminal position when the angle with the receiving optical fiber terminal is 0.

[0089] S2-7 specifically includes:

[0090] S2-7-1: Calculate the rotational proportional absorbance according to the position angle and the second absorbance, wherein the position angle refers to the angle between the positions of different transmitting optical fiber terminals and the initial position.

[0091] S2-7-2: Calculate the difference between the first absorbance and the rotation-ratio absorbance.

[0092] S2-7-3: Determine the fourth eigenvector based on the difference.

[0093] S2-8: Concatenate the first eigenvector, the second eigenvector, the third eigenvector and the fourth eigenvector to obtain a multi-angle rotation spectrum fusion feature, wherein the data dimensions of the first eigenvector, the second eigenvector, the third eigenvector and the fourth eigenvector are the same.

[0094] S3: using the multi-angle rotation spectrum fusion feature as input, and using a concentration detection model to obtain the concentration of the sample solution to be tested.

[0095] As an example, this embodiment is based on FIG. 1(a) and FIG. 1(b), combined with Figure 3 , the above S1-S3 process is explained in detail.

[0096] 1) Inject the fecal bacteria sample to be tested into a centrifuge tube and place it on the centrifuge tube stand.

[0097] 2) Use a light source and a spectrometer to obtain dual-band spectrum data of the sample at position a, where:

[0098] Position a is the position when the angle between the transmitting optical fiber terminal and the receiving optical fiber terminal is 0°. Position a is the position where the transmitting optical fiber terminal and the receiving optical fiber terminal are on the same straight line. After the laser passes through the centrifuge tube, part of it is blocked by the sample, another part is scattered or refracted to other angles, and another part passes through the centrifuge tube and is received by the receiving optical fiber terminal to measure the intensity of the light. This direct light intensity loss is minimal, and the received light intensity is maximum. If there is a certain angle, the light intensity will gradually decrease. When the incident light and the receiving optical fiber terminal are 90 degrees, the received light intensity is the lowest. Most of the light directly penetrates the centrifuge tube and irradiates the opposite side, and only a small part of the light irradiates the bacterial liquid sample and reflects to the 90-degree direction. During the test, the centrifuge tube can be placed at any angle. After it is placed, the transmitting optical fiber terminal rotates from 0°, and the light intensity received by the receiving terminal in the range of 0-90° is continuously tested.

[0099] The dual-band spectral data are visible light band and near-infrared band.

[0100] 3) Rotate the rotating platform clockwise from position a by an angle θ, obtain the dual-band spectrum data of the sample at this new position, and record this position as position k, where k ≥ 1.

[0101] 4) Repeat step 3) to obtain dual-band spectral data of samples at multiple positions.

[0102] 5) Rotate the rotating platform 90° clockwise from position a, record this position as position b, and obtain the dual-band spectrum data of the sample at position b.

[0103] 6) Assuming that step 3 is repeated k (k ≥ 1) times, data at k+2 positions are obtained in total, and these data are called original spectral data; the original spectral data at k+2 positions obtained by the visible light spectrometer (10) and the near infrared spectrometer (11) are preprocessed by a computer, and the preprocessing method is as follows:

[0104] (1) The absorbance at each wavelength is calculated using the original light intensity data. The calculation formula is as follows:

[0105]

[0106] Wherein, A is the absorbance at the current transmitting optical fiber terminal position; I0 is the light intensity value emitted by the transmitting optical fiber terminal at the current transmitting optical fiber terminal position; I1 is the light intensity value received by the receiving optical fiber terminal at the current transmitting optical fiber terminal position; I background It is the background light intensity value at the current transmitting optical fiber terminal position.

[0107] (2) The absorbance A at each wavelength constitutes the absorbance curve l. For the convenience of expression, the absorbance at position a is recorded as A a The corresponding absorbance curve is recorded as la , the absorbance at position b is recorded as A b The corresponding absorbance curve is recorded as l b , the absorbance at position k is recorded as A k The corresponding absorbance curve is recorded as l k ; The absorbance curve of each sample is l a ,l1,l2,…,l k ,l b , a total of k+2, these k+2 absorbance curves are used as the spectral feature 1 of the sample, the x-axis of the absorbance curve is wavelength, and the y-axis is absorbance.

[0108] (3) Calculate the area under each absorbance curve l, denoted as S i , where i=1,2,…,k+2; S1,S2,…,S k+2 As the spectral characteristics of the sample 2.

[0109] 7) Find the peak value of each absorbance curve l, recorded as P i , where i = 1, 2, ..., k + 2; P1, P2, ..., P k+2 As the spectral characteristics of the sample 3.

[0110] 8) Calculate the rotation ratio absorbance A' at each position using the following formula:

[0111]

[0112] Among them, A a is the absorbance at position a, and θ is the angle between the current position and position a.

[0113] At this time, there will be a difference between the absorbance A measured at each position and the rotation ratio absorbance A' calculated at this position, which is recorded as δ i , that is, δ i =A i -A' i , where i = 1, 2, ..., k + 2; the difference values ​​δ1, δ2, ..., δ at each position of the sample are k+2 As the spectral feature 4 of the sample, for example:

[0114] a. When in position 1, θ = 0°, A a =A' a , that is, δ1=0;

[0115] b. When at position k, 0°<θ<90°, δ k =A k -A' k ;

[0116] c. When at position b, θ = 90°, A' b =0, that is, δ k+2 =A b .

[0117] 9) Spectral feature 1, spectral feature 2, spectral feature 3 and spectral feature 4 are fused to obtain a multi-angle rotational spectral fusion feature, and the fusion method is: each feature is spliced ​​end to end in sequence.

[0118] 10) Establishing a concentration detection model, the concentration detection model is: artificial neural network, convolutional neural network or partial least squares regression fitting, etc.

[0119] 11) After the concentration detection model is established, the multi-angle rotation spectrum fusion features and concentration detection model are used to achieve non-destructive detection of fecal bacteria liquid concentration.

[0120] 1. The detection device and method of this embodiment take into account the actual conditions of fecal bacteria liquid, such as turbidity, weak spectral signals, and the need for non-destructive testing. Compared with other existing methods and systems, the detection process is simple and effective.

[0121] 2. This embodiment uses a multi-angle rotation spectroscopy method and device to detect the concentration of fecal bacteria liquid. Its advantage is that any number of different rotation angles can be selected for analysis, which improves the stability and applicability of the system.

[0122] 3. This embodiment proposes an effective data preprocessing method for rotation spectrum. By extracting different features of the rotation spectrum, the macroscopic changes of optical parameters such as absorption coefficient and scattering coefficient of the sample to be tested can be reflected.

[0123] 4. This embodiment uses a self-designed dual-band rotational spectrum detection device and method to detect the concentration of actual fecal bacteria liquid. Compared with the existing methods, it does not require complicated sampling, dilution or cultivation operations. It only needs to put the sample to be tested into the detection system, which can achieve the purpose of non-destructive detection of fecal bacteria liquid concentration.

[0124] 5. The data processing and modeling method used in this embodiment uses four spectral features in two bands, which greatly increases the amount of information compared with the currently commonly used spectral method for detecting concentration, thereby effectively improving the detection accuracy.

[0125] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0126] This article uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only used to help understand the method and core ideas of this application. At the same time, for those skilled in the art, according to the ideas of this application, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting this application.

Claims

1. A bacterial liquid concentration detection device based on multi-angle rotation spectrum, characterized in that: The bacterial liquid concentration detection device based on multi-angle rotation spectrum includes: Light source, transmitting optical fiber terminal, centrifuge tube, receiving optical fiber terminal, spectrometer, rotating platform and computer; The light source is connected to the transmitting optical fiber terminal via an optical fiber; The receiving optical fiber terminal is connected to the spectrometer via an optical fiber; The spectrometer is connected to the computer; The light source is used to provide photons in different wavelength bands; The centrifuge tube is used to hold the sample solution to be tested; The transmitting optical fiber terminal is used to irradiate photons to the sample solution to be tested; The receiving optical fiber terminal is used to receive photons transmitted from the sample solution to be tested; The transmitting optical fiber terminal and the centrifuge tube are both located on the rotating platform; The rotating platform is used to drive the transmitting optical fiber terminal to rotate; The spectrometer is used to obtain original spectral data according to the photons transmitted by the sample solution to be tested, wherein the original spectral data includes spectral data of different wavelength ranges at different emission optical fiber terminal positions; The computer is used to extract multi-angle rotation spectrum fusion features according to the original spectrum data; and use the multi-angle rotation spectrum fusion features as input to obtain the concentration of the sample solution to be tested using a concentration detection model.

2. The bacterial liquid concentration detection device based on multi-angle rotation spectrum according to claim 1 is characterized in that: The spectrometer includes a visible light spectrometer and a near infrared light spectrometer; The visible light spectrometer and the near infrared light spectrometer are respectively connected to the computer; The visible light spectrometer and the near infrared light spectrometer are respectively connected to the receiving optical fiber terminal through optical fibers; The visible light spectrometer is used to obtain visible light spectrum data at different emission optical fiber terminal positions according to the visible light photons transmitted by the sample solution to be tested; The near-infrared spectrometer is used to obtain near-infrared light spectrum data at different emission optical fiber terminal positions according to the near-infrared photons transmitted by the sample solution to be tested.

3. The bacterial liquid concentration detection device based on multi-angle rotation spectrum according to claim 1 is characterized in that: The bacterial liquid concentration detection device based on multi-angle rotation spectrum also includes: a transmitting optical fiber terminal bracket and a receiving optical fiber terminal bracket; The transmitting optical fiber terminal bracket is used to fix the transmitting optical fiber terminal; The receiving optical fiber terminal bracket is used to fix the receiving optical fiber terminal; The transmitting optical fiber terminal bracket is located on the rotating platform.

4. The bacterial liquid concentration detection device based on multi-angle rotation spectrum according to claim 1, characterized in that: The bacterial liquid concentration detection device based on multi-angle rotation spectrum also includes: a centrifuge tube support; the centrifuge tube support is used to fix the centrifuge tube; the centrifuge tube support is located on the rotating platform.

5. The bacterial liquid concentration detection device based on multi-angle rotation spectrum according to claim 3 is characterized in that: The bacterial liquid concentration detection device based on multi-angle rotation spectrum also includes a base; the rotating platform and the receiving optical fiber terminal bracket are both fixed on the base.

6. A method for detecting bacterial liquid concentration based on multi-angle rotation spectrum, characterized in that: The bacterial liquid concentration detection method based on multi-angle rotation spectrum is implemented by using the bacterial liquid concentration detection device based on multi-angle rotation spectrum according to any one of claims 1 to 5, and the bacterial liquid concentration detection method based on multi-angle rotation spectrum includes: Acquire original spectral data of the sample solution to be tested, wherein the original spectral data includes spectral data of different wavelength bands at different transmitting optical fiber terminal positions; Extracting multi-angle rotation spectrum fusion features according to the original spectrum data; The multi-angle rotation spectrum fusion feature is used as input, and the concentration of the sample solution to be tested is obtained using a concentration detection model.

7. The method for detecting bacterial liquid concentration based on multi-angle rotation spectrum according to claim 6, characterized in that: According to the original spectral data, extracting multi-angle rotation spectral fusion features specifically includes: According to the original spectral data, an absorbance curve data group is obtained, wherein the absorbance curve data group includes absorbance curves at a plurality of different transmitting optical fiber terminal positions, and each absorbance curve refers to a curve determined according to the absorbance of photons in different wavelength ranges at the same transmitting optical fiber terminal position; Determine a first eigenvector according to the absorbance curve data set; Calculating the area of ​​a target graph, wherein the target graph refers to a graph enclosed by each of the absorbance curves and the coordinate axis; determining a second eigenvector based on the area; Determine the peak point in each absorbance curve; Determine a third eigenvector according to the peak point; Determine a fourth eigenvector according to the first absorbance and the second absorbance, wherein the first absorbance refers to the absorbance of photons in different wavelength ranges at different transmitting optical fiber terminal positions, and the second absorbance refers to the absorbance of photons in different wavelength ranges at an initial position, and the initial position refers to the transmitting optical fiber terminal position when the angle with the receiving optical fiber terminal is 0; The first eigenvector, the second eigenvector, the third eigenvector and the fourth eigenvector are concatenated to obtain a multi-angle rotation spectrum fusion feature, wherein the data dimensions of the first eigenvector, the second eigenvector, the third eigenvector and the fourth eigenvector are the same.

8. The method for detecting bacterial liquid concentration based on multi-angle rotation spectrum according to claim 7, characterized in that: Determining a fourth eigenvector according to the first absorbance and the second absorbance specifically includes: Calculate the rotational proportional absorbance according to the position angle and the second absorbance, wherein the position angle refers to the angle between the positions of different transmitting optical fiber terminals and the initial position; calculating a difference between the first absorbance and the rotation-scale absorbance; A fourth eigenvector is determined based on the difference.

9. The method for detecting bacterial liquid concentration based on multi-angle rotation spectrum according to claim 8, characterized in that: The calculation formula of the rotation ratio absorbance is: Where A' is the rotational proportional absorbance at the current transmitting optical fiber terminal position; A a is the second absorbance; θ is the angle between the current transmitting optical fiber terminal position and the initial position.

10. The method for detecting bacterial liquid concentration based on multi-angle rotation spectrum according to claim 7, characterized in that: The calculation formula of the absorbance is: Wherein, A is the absorbance at the current transmitting optical fiber terminal position; I0 is the light intensity value emitted by the transmitting optical fiber terminal at the current transmitting optical fiber terminal position; I1 is the light intensity value received by the receiving optical fiber terminal at the current transmitting optical fiber terminal position; I background It is the background light intensity value at the current transmitting optical fiber terminal position.

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