Blood fat test card based on photochemical principle and blood fat detection method

Abstract

The invention discloses a blood fat test card based on a photochemical principle, and belongs to the technical field of in-vitro detection. The blood fat detection card comprises a card body, the card body is provided with a sample adding port, a correction area and at least one test area, the sample adding port is respectively communicated with the correction area and the test area, and the correction area and the test area are respectively provided with an air hole communicated with the outside; the correction area is provided with a reaction film and a hemolytic agent; and the test area is provided with an enzyme layer and an interference removing film corresponding to a to-be-detected item. The photochemical principle is adopted, the reflected light values of the test area and the correction area before and after the reaction are obtained, the luminosity value difference related to the initial concentration and hematocrit of the item to be detected is obtained, the concentration value of the item to be detected is corrected through the luminosity value difference related to the hematocrit, and therefore the corrected concentration value of the item to be detected is calculated. The detection result is more accurate and reliable, and the operation is simple.

Description

Technical Field

[0001] The present invention relates to the technical field of blood lipid detection, and in particular to a blood lipid test card and a blood lipid detection method based on photochemical principle. Background Art

[0002] The rapid detection system product consisting of blood lipid test cards and blood lipid analyzers can quickly and accurately detect patients' blood lipid levels, provide early warning for potential patients with hyperlipidemia, and provide a scientific basis for accurate diagnosis and rational use of drugs. It is now widely used in the medical system.

[0003] However, the existing blood lipid test cards and test methods still have the following problems in actual use:

[0004] 1. The blood lipid test card described in "A blood lipid multi-function test card with precise control structure" disclosed in Chinese patent application number CN202310402379.8 is complicated to operate. After blood collection, it is necessary to use a quantitative adding tool for adding samples. The test results are affected by the user's operating proficiency. Pauses during the adding process and too fast or too slow adding speeds will affect the amount of samples flowing to each reaction well, which may cause the test results to be prone to abnormalities.

[0005] 2. For example, the blood lipid test strip described in the "Blood lipid three-item test strip and preparation method thereof" disclosed in Chinese patent application number CN201721135281.7 can achieve self-priming function through a hydrophilic membrane structure, but the anti-hematocrit data disclosed therein show that when the hematocrit is 45%, the concentration deviation exceeds -10%; when the hematocrit is 55%, the concentration deviation is close to -20%. It can be seen that the effect of hematocrit is not corrected or the correction effect is poor, resulting in inaccurate final test results.

[0006] In view of this, it is necessary to propose a new technical solution to solve the above technical problems. Summary of the invention

[0007] The object of the present invention is to provide a blood lipid detection card and a blood lipid detection method to solve the problems existing in the above-mentioned prior art.

[0008] To achieve the above purpose, the present invention adopts the following technical means:

[0009] The first aspect of the present invention provides a blood lipid test card based on the photochemical principle, comprising a card body, wherein the card body is provided with a sample addition port, a calibration area and at least one test area, wherein the sample addition port is connected to the calibration area and the test area respectively, and the calibration area and the test area are both provided with air holes connected to the outside world;

[0010] The calibration area is provided with a reaction membrane and a hemolytic agent, and the calibration area is used to detect the photometric value difference related to the hematocrit in the blood sample;

[0011] The test area is provided with an enzyme layer and an interference removal membrane corresponding to the item to be tested, and the test area is used to detect the photometric value difference related to the initial concentration of the item to be tested in the blood sample.

[0012] As a further improvement, the card body includes a bottom plate and an upper cover that can be attached together;

[0013] The bottom plate is provided with a plurality of grooves, and the plurality of grooves form the calibration area and the test area correspondingly;

[0014] The cover plate is provided with a notch and a plurality of flow channels, and the notch forms the sample addition port;

[0015] One ends of the plurality of flow channels are connected to the notch, and the other ends of the plurality of flow channels are connected to the plurality of grooves respectively;

[0016] The air hole is arranged on the cover plate, and the air hole is connected to the corresponding flow channel.

[0017] As a further improvement, the items to be tested include one or more of total cholesterol, triglycerides, and high-density lipoprotein cholesterol;

[0018] The enzyme layer includes a reaction enzyme corresponding to the item to be tested.

[0019] As a further improvement, the reaction enzyme corresponding to the total cholesterol includes 0.3-0.5U of cholesterol oxidase, 0.2-0.3U of cholesterol esterase and 1-1.2U of peroxidase;

[0020] The reaction enzymes corresponding to the triglyceride mainly include 0.2-0.3U cholesterol oxidase, 0.1-0.2U cholesterol esterase and 0.5-0.6U peroxidase;

[0021] The reaction enzyme corresponding to the high-density lipoprotein cholesterol comprises 0.8-1U lipoprotein esterase, 0.5-0.7U glycerol kinase, 0.4-5U glycerol triphosphate oxidase and 0.5-0.6U peroxidase.

[0022] As a further improvement, the test area further comprises a sunscreen, and the sunscreen comprises zinc oxide or hydrated magnesium silicate.

[0023] As a further improvement, the enzyme layer also includes a color developer, an auxiliary color developer, a buffer, a surfactant, a protective agent, a film-forming agent and a preservative.

[0024] As a further improvement, the material of the interference removal membrane includes glass fiber membrane or polysulfone membrane;

[0025] The interference removal film includes at least one film layer with holes.

[0026] As a further improvement, the interference-removing membrane corresponding to the total cholesterol and triglyceride is labeled with ascorbic acid oxidase and bilirubin oxidase;

[0027] The interference-free membrane corresponding to the high-density lipoprotein cholesterol is labeled with ascorbic acid oxidase, bilirubin oxidase, phosphotungstic acid and magnesium ions.

[0028] As a further improvement, the reaction membrane includes a nylon membrane or a PES membrane with holes; the hemolytic agent includes sodium deoxycholate, nitrite or phosphate buffer.

[0029] A second aspect of the present invention provides a blood lipid detection method using the blood lipid test card as described above, used together with an optical detector, wherein the blood sample comprises whole blood or serum, and the method comprises the following steps:

[0030] Obtain the reflectance luminosity value λ0 of the test area before the reaction and the reflectance luminosity value λ after the reaction, calculate the luminosity value difference Δλ before and after the reaction = λ0-λ, and calculate the initial concentration value M0 of the item to be tested corresponding to the test area according to the following equation:

[0031] M 0血清 =A 血清 *Δλ+B 血清 (1)

[0032] M 0全血 =A 全血 *Δλ+B 全血 (2)

[0033] Get the reflectance value λ before adding sample to the calibration area HB0 , and the reflectance value λ after the reaction HB , calculate the difference in reflectance value Δλ before and after the reaction HB =λ HB0 -λ HB , according to the luminosity difference Δλ HB The size of the blood sample determines whether it is serum or whole blood.

[0034] As Δλ HB ≤0.15λ HB0 , then it is judged to be serum, then the final concentration value of the item to be tested corresponding to the test area is equal to the initial concentration value M 0血清 ;

[0035] As Δλ HB >0.15λ HB0 , then it is judged to be whole blood, and the final concentration value M of the item to be tested corresponding to the test area is calculated according to the following equation:

[0036] M= M 0全血 *(C*Δλ HB 2 + D*Δλ HB +E) (3)

[0037] The A, B, C, D and E are all batch-specific fitting constants.

[0038] Compared with the prior art, the present invention brings the following technical effects:

[0039] The present invention adopts the principle of photochemistry to obtain the reflected light values ​​of the test area and the calibration area before and after the reaction, obtains the photometric value difference related to the initial concentration and hematocrit of the item to be tested, and corrects the concentration value of the item to be tested by the photometric value difference related to the hematocrit, thereby calculating the corrected concentration value of the item to be tested. The method can effectively reduce the interference of hematocrit in the blood sample and improve the accuracy of the blood lipid detection result through actual measurement, and can detect whole blood and serum, expanding the application scope of the blood lipid detection card; in addition, the sample inlet of the blood lipid detection card is connected with the test area and the calibration area, and a blood sample can be evenly distributed without delay through the siphon effect, ensuring the high consistency of the samples obtained in each detection area, and the operation is simple. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0041] Figure 1 A top view of a blood lipid detection card according to a preferred embodiment of the present invention is shown;

[0042] Figure 2 A perspective exploded view of the present invention from a first viewing angle is shown;

[0043] Figure 3 A perspective exploded view of the present invention from a second viewing angle is shown.

[0044] Description of main component symbols:

[0045] Card body-10; bottom plate-11; groove-111; upper cover-12; flow channel-121; diffusion grid-122; sample adding port-20; calibration area-30; test area-40; air hole-50. DETAILED DESCRIPTION

[0046] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0047] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other. The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements with the same or similar functions.

[0048] Example

[0049] This embodiment provides a blood lipid detection card based on the photochemical principle. The photochemical principle means that the detection card needs to be used in conjunction with an optical detector. The optical detector emits incident light to the detection area of ​​the blood lipid detection card on the one hand, and receives reflected light from the detection area on the other hand, and obtains the detection result by analyzing the changes in the light before and after. This detection technology based on the photochemical principle is quite common in the field of in vitro detection. The present invention will directly quote the relevant existing technologies in this field without making too many elaborations.

[0050] See also Figures 1 to 3 Specifically in this embodiment, the blood lipid test card includes a card body 10, and the card body 10 is provided with a sample addition port 20, a calibration area 30 and three test areas 40; the sample addition port 20 is connected with the calibration area 30 and the three test areas 40 respectively, and the calibration area 30 and the three test areas 40 are provided with air holes 50 connected with the outside.

[0051] More specifically, the sample adding port 20 is located at the edge of the card body 10, and the calibration area 30 and the three test areas 40 are distributed inside the card body 10, and are separated from each other by a certain distance. Because the calibration area 30 and the three test areas 40 will react during use, these areas can be collectively referred to as reaction areas for the convenience of description. There are fine channels between the sample adding port 20 and each reaction area, and these fine channels can establish a siphon effect between the reaction area and the sample adding port 20. Therefore, after the blood sample is dripped into the sample adding port 20, it will quickly flow into the reaction area due to the siphon effect. Compared with the blood lipid detection card structure in the prior art that requires dripping blood samples into the reaction areas separately, the present embodiment can complete the time-free and uniform distribution of multiple reaction areas to a blood sample at one time, ensuring the high consistency of the sample obtained in each reaction area, and the operation is simpler, thereby ensuring the credibility of the test results.

[0052] Specifically in this embodiment, the calibration area 30 is provided with a reaction membrane and a hemolytic agent, and the calibration area 30 is used to detect the photometric value difference related to the hematocrit in the blood sample. The three test areas 40 are provided with enzyme layers and interference removal membranes corresponding to the items to be tested, and the test areas 40 are used to detect the photometric value difference related to the initial concentration of the items to be tested in the blood sample. In this embodiment, the three test areas 40 correspond to the detection of three indicators: total cholesterol, triglycerides, and high-density lipoprotein cholesterol. The specific reaction process and test method of the above reaction areas are detailed below.

[0053] Please continue reading Figure 2 and Figure 3 , which shows a three-dimensional exploded view of a blood lipid test card. Specifically, the card body 10 includes a bottom plate 11 and an upper cover 12 that can be bonded together, and the bonding method includes ultrasonic welding or gluing.

[0054] Specifically, the bottom plate 11 is provided with a plurality of grooves 111, and the plurality of grooves 111 correspond to form a calibration area 30 and three test areas 40; the upper cover 12 is provided with a notch and a plurality of flow channels 121, and the notch forms a sample addition port 20, and one end of the plurality of flow channels 121 are connected to the notch, and the other ends of the plurality of flow channels 121 are respectively connected to the plurality of grooves 111; the air hole 50 is provided on the upper cover 12, and the air hole 50 is connected to the corresponding flow channel 121. After the upper cover 12 and the bottom plate 11 are bonded together, a finished product of a blood lipid detection card is formed. Of course, before bonding, the reaction membrane, hemolytic agent, enzyme layer and interference removal membrane of each reaction area need to be loaded into the corresponding groove 111. The sample injection port 20 of this embodiment passes through the upper cover 12 and the bottom plate 11. Of course, in some other embodiments, the sample injection port 20 can also be set to pass through only the upper cover 12. The plurality of flow channels 121 can be set to share a section of the flow channel (such as Figure 2 As shown), it can also be set to not share the flow channel.

[0055] Preferably, a diffusion grid 122 is further provided on the upper cover 12 corresponding to each groove 111 . The diffusion grid 122 and the groove 111 together form a reaction zone. The diffusion grid 122 facilitates the blood sample to diffuse more quickly to the entire reaction zone.

[0056] Description of reactants in each reaction zone

[0057] The specific application scenario of this embodiment is the detection of three blood lipids, so there are three test areas 40, corresponding to the detection of the concentrations of total cholesterol, triglycerides, and high-density lipoprotein cholesterol.

[0058] The reactants in the test area 40 all include an enzyme layer and an interference removal membrane, wherein the interference removal membranes in the three test areas 40 all use at least one porous membrane layer, the apertures of the multiple membrane layers may be aligned vertically or not, and the membrane material includes a glass fiber membrane or a polysulfone membrane.

[0059] The enzyme layer contains reaction enzymes and some auxiliary reagents. Specifically, the reaction enzymes corresponding to total cholesterol include 0.3-0.5U cholesterol oxidase, 0.2-0.3U cholesterol esterase and 1-1.2U peroxidase; the reaction enzymes corresponding to triglycerides mainly include 0.2-0.3U cholesterol oxidase, 0.1-0.2U cholesterol esterase and 0.5-0.6U peroxidase; the reaction enzymes corresponding to high-density lipoprotein cholesterol include 0.8-1U lipoprotein esterase, 0.5-0.7U glycerol kinase, 0.4-5U glycerol triphosphate oxidase and 0.5-0.6U peroxidase. After the above reaction enzymes come into contact with the blood sample, they react with the corresponding items to be tested, thereby causing the difference in incident light before and after the reaction, thereby providing information on the concentration of the items to be tested.

[0060] The reaction enzyme is matched with each item to be tested, and the auxiliary reagents can be universal. Generally, the auxiliary reagents include colorimetric agents, auxiliary colorimetric agents, buffers, surfactants, protective agents, film-forming agents and preservatives. These auxiliary reagents are used to ensure that the test results are better from some minor aspects. Therefore, those skilled in the art can appropriately increase, decrease and adjust them according to actual conditions.

[0061] Preferably, in this embodiment, a sunscreen is added to the auxiliary reagent, such as zinc oxide or hydrated magnesium silicate. The sunscreen can block the light-transmitting film layer, prevent the color of red blood cells on the interference film from absorbing light, causing the reflected light to decrease, thereby affecting the test value of the product, so the use of the sunscreen can improve the test results.

[0062] In this embodiment, the interference removal membrane corresponding to total cholesterol and triglycerides is labeled with ascorbic acid oxidase and bilirubin oxidase; the interference removal membrane corresponding to high-density lipoprotein cholesterol is labeled with ascorbic acid oxidase, bilirubin oxidase, phosphotungstic acid and magnesium ions. These reactants can specifically eliminate the interference of other factors in the blood sample, which is conducive to improving the accuracy of the final test results.

[0063] The reaction membrane of the calibration area 30 is a nylon membrane or a PES membrane with holes, and the hemolytic agent includes sodium deoxycholate, nitrite or phosphate buffer. After the blood sample enters the calibration area 30, it reacts with the hemolytic agent, thereby causing a difference in the incident light before and after the reaction, thereby providing information related to the hematocrit in the blood sample.

[0064] Detection process and principle description

[0065] The blood lipid detection card of the present invention needs to be used together with an optical detector, and the two together form a complete blood lipid in vitro detection system. The blood sample to be tested does not need to be distinguished between whole blood and serum, thus expanding the scope of application of the blood lipid detection card. The details are as follows:

[0066] Obtain the reflectance luminosity value λ0 of the test area 40 before the reaction and the reflectance luminosity value λ after the reaction, calculate the luminosity value difference Δλ before and after the reaction = λ0-λ, and calculate the initial concentration value M0 of the item to be tested corresponding to the test area 40 according to the following equation:

[0067] M 0血清 =A 血清 *Δλ+B 血清 (1)

[0068] M 0全血 =A 全血 *Δλ+B 全血 (2)

[0069] Get the reflectance value λ of the calibration area 30 before adding the sample HB0 , and the reflectance value λ after the reaction HB , calculate the difference in reflectance value Δλ before and after the reaction HB =λ HB0 -λ HB , according to the luminosity difference Δλ HB The size of the blood sample determines whether it is serum or whole blood.

[0070] As Δλ HB ≤0.15λ HB0 , it is determined to be serum, then the final concentration value of the item to be tested corresponding to the test area 40 is equal to the initial concentration value M 0血清 ;

[0071] As Δλ HB >0.15λ HB0 , then it is determined to be whole blood, and the final concentration value M of the item to be tested corresponding to the test area 40 is calculated according to the following equation:

[0072] M= M 0全血 *(C*Δλ HB 2 + D*Δλ HB +E) (3)

[0073] The A, B, C, D and E are all batch-specific fitting constants.

[0074] Specifically, the step of obtaining the photometric value difference of each reaction area before and after the reaction can be performed as follows: the blood lipid test card is placed in the observation area of ​​the optical detector, and the optical detector irradiates each reaction area of ​​the blood lipid test card with incident light of a preset wavelength, and obtains the reflection photometric value λ0 of the test area 40 before the reaction and the reflection photometric value λ0 of the calibration area 30 before the sample is added. HB0 Drop the blood sample in, wait for the reactant to react with the blood sample fully, and obtain the reflectance value λ of the test area 40 after the reaction and the reflectance value λ of the calibration area 30 after the sample is added. HB——Then we get Δλ and Δλ HB .

[0075] It should be noted that the wavelength of the incident light of the three test areas 40 may be 620-630 nm, preferably 625 nm is the best wavelength of the incident light. The wavelength of the incident light of the calibration area 30 may be 520-530 nm, preferably 525 nm is the best wavelength of the incident light.

[0076] In addition, the constants in formulas (1)-(3) can be obtained by performing linear fitting after multiple actual measurements of samples with known concentrations and known hematocrit. This method of obtaining formula constants is a common method in the industry and will not be described in detail in this embodiment. For example, it can be obtained as follows:

[0077] A, B acquisition method: select 9 to 11 serum samples with known actual concentrations or whole blood samples with a hematocrit of 42% covering the test range, collect the photometric value differences of the corresponding test areas for multiple times by the present invention, and calculate the average of the photometric value differences by As the horizontal axis, the actual concentration of the known sample is used as the vertical axis for linear fitting. The slope of the linear equation is A, and the intercept is B. In this way, we can obtain A of formula (1): 血清、 B 血清 , and A in formula (2) 全血、 B 全血 .

[0078] The establishment method of C, D, and E is as follows: select medium-concentration venous whole blood, divide it into eight equal parts, adjust the proportion of red blood cells, and make the hematocrit of these eight blood samples 20%, 25%, 30%, 35%, 42%, 50%, 55%, and 60% respectively. The initial concentration M0 whole blood and the photometric difference with the calibration area of ​​these eight samples are obtained multiple times by the present invention. And calculate the average initial concentration of each sample The mean value of the photometric difference between the sum and the calibration area The average initial concentration of the test area with a hematocrit of 42% was taken as the intermediate hematocrit concentration to obtain the intermediate hematocrit concentration. The ratio coefficient K between each compressed concentration is by is the X value, the corresponding K is the Y value, and a quadratic equation is fitted. Get C, D, E.

[0079] Measured data

[0080] The following six groups of experiments are used to verify the effectiveness of the blood lipid detection card and the blood lipid detection method of the present invention. The six groups of experiments correspond to known concentrations of low-concentration total cholesterol, high-concentration total cholesterol, low-concentration high-density lipoprotein cholesterol, high-concentration high-density lipoprotein cholesterol, low-concentration triglycerides, and high-concentration triglycerides. Each group of experiments uses blood samples containing different hematocrit values ​​(known), and the blood samples include whole blood and serum.

[0081] The test data of the above six groups of experiments are recorded as follows:

[0082]

[0083]

[0084]

[0085]

[0086] Note: In the above table, the hematocrit value of 42% corresponds to the blood sample of a normal person.

[0087] From the above six sets of experimental data, we can see that:

[0088] 1. The measurement results without hematocrit correction cannot measure serum, but the present invention can measure serum;

[0089] 2. In the measurement results without hematocrit correction, the average value AV of the measurement results obtained shows great divergence, whether it is the deviation from the normal value or the deviation from the reference value. However, in the measurement results after hematocrit correction of the present invention, the average value AV shows excellent convergence, whether it is the deviation from the normal value or the deviation from the reference value.

[0090] It can be seen that in the scenario of in vitro blood lipid detection, the measurement results after hematocrit correction using the present invention are more authentic and accurate.

[0091] Other Notes

[0092] The above embodiment is provided with three test areas, which are for the scenario of three routine blood lipid items. However, according to the purpose of the present invention, in some other embodiments, at least one test area can be set, such as one test area, two test areas, or four or more test areas; but no matter how it is set, the correction area is necessary.

[0093] Obviously, it is not necessary and impossible to list all the embodiments here, and the obvious changes or modifications derived therefrom still fall within the protection scope of the invention.

Claims

1. A blood lipid test card based on photochemical principle, characterized in that: The card body comprises a sample adding port, a calibration area and at least one test area, the sample adding port is connected to the calibration area and the test area respectively, and the calibration area and the test area are both provided with air holes connected to the outside; The calibration area is provided with a reaction membrane and a hemolytic agent, and the calibration area is used to detect the photometric value difference related to the hematocrit in the blood sample; The test area is provided with an enzyme layer and an interference removal membrane corresponding to the item to be tested, and the test area is used to detect the photometric value difference related to the initial concentration of the item to be tested in the blood sample.

2. The blood lipid detection card according to claim 1, characterized in that: The card body includes a bottom plate and an upper cover that can be attached together; The bottom plate is provided with a plurality of grooves, and the plurality of grooves form the calibration area and the test area correspondingly; The cover plate is provided with a notch and a plurality of flow channels, and the notch forms the sample addition port; One ends of the plurality of flow channels are connected to the notch, and the other ends of the plurality of flow channels are connected to the plurality of grooves respectively; The air hole is arranged on the cover plate, and the air hole is connected to the corresponding flow channel.

3. The blood lipid detection card according to claim 1, characterized in that: The items to be tested include one or more of total cholesterol, triglycerides, and high-density lipoprotein cholesterol; The enzyme layer includes a reaction enzyme corresponding to the item to be tested.

4. The blood lipid detection card according to claim 3, characterized in that: The reaction enzymes corresponding to the total cholesterol include 0.3-0.5U cholesterol oxidase, 0.2-0.3U cholesterol esterase and 1-1.2U peroxidase; The reaction enzymes corresponding to the triglyceride mainly include 0.2-0.3U cholesterol oxidase, 0.1-0.2U cholesterol esterase and 0.5-0.6U peroxidase; The reaction enzyme corresponding to the high-density lipoprotein cholesterol comprises 0.8-1U lipoprotein esterase, 0.5-0.7U glycerol kinase, 0.4-5U glycerol triphosphate oxidase and 0.5-0.6U peroxidase.

5. The blood lipid detection card according to claim 1, characterized in that: The test area also includes a sunscreen comprising zinc oxide or hydrous magnesium silicate.

6. The blood lipid detection card according to claim 5, characterized in that: The enzyme layer also includes a color developing agent, an auxiliary color developing agent, a buffer, a surfactant, a protective agent, a film forming agent and a preservative.

7. The blood lipid detection card according to claim 1, characterized in that: The material of the interference removal membrane includes glass fiber membrane or polysulfone membrane; The interference removal film includes at least one film layer with holes.

8. The blood lipid detection card according to claim 3, characterized in that: The interference-free membrane corresponding to the total cholesterol and triglyceride is labeled with ascorbic acid oxidase and bilirubin oxidase; The interference-free membrane corresponding to the high-density lipoprotein cholesterol is labeled with ascorbic acid oxidase, bilirubin oxidase, phosphotungstic acid and magnesium ions.

9. The blood lipid detection card according to claim 1, characterized in that: The reaction membrane includes a nylon membrane or a PES membrane with holes; the hemolytic agent includes sodium deoxycholate, nitrite or phosphate buffer.

10. A method for detecting blood lipids using the blood lipid test card according to any one of claims 1 to 9, used together with an optical detector, wherein the blood sample comprises whole blood or serum, characterized in that: The following steps are involved: Obtain the reflectance luminosity value λ0 of the test area before the reaction and the reflectance luminosity value λ after the reaction, calculate the luminosity value difference Δλ before and after the reaction = λ0-λ, and calculate the initial concentration value M0 of the item to be tested corresponding to the test area according to the following equation: M 0血清 =A 血清 *Dl+B 血清 (1) M 0全血 =A 全血 *Dl+B 全血 (2) Get the reflectance value λ before adding sample to the calibration area HB0 , and the reflectance value λ after the reaction HB , calculate the difference in reflectance value Δλ before and after the reaction HB =λ HB0 -λ HB , according to the luminosity difference Δλ HB The size of the blood sample determines whether it is serum or whole blood. As Δλ HB ≤0.15λ HB0 , then it is judged to be serum, then the final concentration value of the item to be tested corresponding to the test area is equal to the initial concentration value M 0血清 ; As Δλ HB >0.15λ HB0 , then it is judged to be whole blood, and the final concentration value M of the item to be tested corresponding to the test area is calculated according to the following equation: M=M 0全血 *(C*Dl HB 2 +D*Dl HB +E) (3) The A, B, C, D and E are all batch-specific fitting constants.

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