Unattended full-automatic immunoblotting detection system and method

Abstract

The invention relates to the technical field of immunoblotting detection, in particular to an unattended full-automatic immunoblotting detection system and method. After the sample adding needle is cleaned, part of the cleaning liquid stays on the surface of the sample adding needle, and the sample adding needle needs to move upwards to reset after the sample adding needle is cleaned, so that the mounting pipe and the cleaning plate are in a static state in the upward moving process of the sample adding needle; the sample adding needle, the mounting pipe and the cleaning plate are in a relative motion state, so that the sample adding needle moves upwards in the through hole in the middle of the cleaning plate, and the inner wall of the through hole is in close contact with the outer side of the sample adding needle in the process, so that the cleaning plate scrapes cleaning liquid on the surface of the sample adding needle; the problem of cross infection generated when the sample adding needle samples the to-be-detected sample is avoided, so that the detection accuracy is improved.

Description

Technical Field

[0001] The invention relates to the technical field of immunoblotting detection, in particular to an unattended fully automatic immunoblotting detection system and method. Background Art

[0002] Immunoblotting analyzer, also known as Western Blot analyzer, uses immunoblotting technology to complete the entire process of sample processing, immune response, detection and result analysis. It is a high-throughput laboratory analysis equipment;

[0003] The principle of immunoblot analyzer is;

[0004] Sample preparation: Extract the protein of the sample to be tested, lyse it and separate the protein.

[0005] Electrophoretic separation: The separated proteins are separated by electrophoresis on the gel according to their molecular weight to form a protein spectrum in the form of strips.

[0006] Membrane transfer: Transfer the proteins on the gel to a nitrocellulose membrane (NC membrane) or PVDF membrane to form protein bands.

[0007] Blocking: Block nonspecific binding sites on the membrane to prevent antibodies from binding to non-target proteins.

[0008] Antibody incubation: The labeled specific antibody is incubated with the target protein on the membrane to allow the antibody to bind to the target protein.

[0009] Wash: Wash away unbound antibody to increase signal intensity.

[0010] Color development: Depending on the type of labeled antibody, use the corresponding color developing reagent to display color at the site where the antibody binds to the target protein.

[0011] Detection signal: The developed membrane is scanned and analyzed by an instrument to obtain the signal intensity of the target protein.

[0012] Result analysis: The system automatically analyzes the signal intensity and compares it with the standard to obtain the content or activity of the target protein.

[0013] However, the existing detection methods for immunoblotting are divided into two categories: manual detection and instrument detection. The manual detection method requires the replacement of different reagents. Frequent operations increase the workload of the experimenter and waste time. In addition, manual operations are tedious and complicated, people have more contact with potentially infectious items, and the detection safety is poor.

[0014] In summary, in order to solve the technical problems raised in this article, the present invention proposes an unattended fully automatic immunoblotting detection system and method. Summary of the invention

[0015] The present invention proposes an unattended fully automatic immunoblotting detection system, which comprises an automatic immunoblotting detector, which comprises an instrument body, on which a sample pool, a reagent pool and a reaction pool are provided;

[0016] A sample disk is rotatably connected inside the sample pool, and a plurality of sample holes are provided on the sample disk;

[0017] The reagent pool is rotatably connected to a reagent disk, the reagent disk is provided with a refrigeration device, and the reagent disk is provided with a plurality of reagent kit placement slots;

[0018] The reaction pool is rotatably connected to an incubation tray, the incubation tray is provided with a heating device, and a plurality of reaction vessel placement slots are provided on the incubation tray;

[0019] A rotating column No. 1 is arranged between the sample pool and the reaction pool, a limiting column No. 1 is arranged at the center of the upper end of the rotating column No. 1, and a telescopic rod is eccentrically arranged at the upper end of the rotating column No. 1; a sample adding plate is slidably connected to the limiting column No. 1, and the sample adding plate is connected to the output end of the telescopic rod, and a sample adding needle is arranged at the lower end of the sample adding plate away from the limiting column No. 1;

[0020] A No. 2 rotating column is arranged between the reagent pool and the reaction pool, a No. 2 limiting column is arranged at the center of the upper end of the No. 2 rotating column, a telescopic rod is eccentrically arranged at the upper end of the No. 2 rotating column, a reagent plate is slidably connected to the No. 2 limiting column, and the reagent plate is connected to the output end of the telescopic rod; a reagent needle is arranged at the lower end of the reagent plate away from the No. 2 limiting column;

[0021] A No. 3 rotating column is arranged on one side of the reaction pool, a telescopic rod is arranged on the No. 3 rotating column, a waste liquid recovery plate is arranged on the telescopic rod of the No. 3 rotating column, and a waste liquid recovery needle is arranged at the lower end of the waste liquid recovery plate; a waste liquid recovery hole is opened at the upper end of the instrument body; a waste liquid recovery box is arranged inside the instrument body, and the waste liquid recovery box is connected with the waste liquid recovery hole through a hose;

[0022] The upper end of the instrument body is provided with two cleaning grooves. In the initial state, one of the cleaning grooves is located directly below the sample injection needle, and the other cleaning groove is located directly below the reagent needle. A cleaning liquid storage box is provided inside the instrument body, and the cleaning liquid storage box is connected to the cleaning groove through a hose. A nozzle is provided inside the cleaning groove, and the nozzle is connected to the cleaning liquid storage box through a hose.

[0023] An arc plate is arranged on the instrument body, a telescopic rod is arranged below one side of the arc plate, the telescopic rod below the arc plate is arranged on the instrument body, an ultrasonic vibrator and a reading and analysis module are arranged on the other side of the arc plate; the ultrasonic vibrator and the reading and analysis module are located above the incubation tray.

[0024] As a preferred solution of the present application; the upper ends of the No. 1 limiting column and the No. 2 limiting column are both provided with mounting plates, the mounting plate on the No. 1 limiting column is located directly above the sample adding plate, and the mounting plate on the No. 2 limiting column is located directly above the reagent plate, and the two mounting plates are both provided with mounting tubes at one end away from the telescopic rod, and a slide groove is provided on the side of the mounting tube; the mounting tube on the No. 1 limiting column passes through the sample adding plate and is located below the sample adding plate, the mounting tube on the No. 1 limiting column is slidably connected to the sample adding plate, and the sample adding needle is located in the middle of the mounting tube, and the part between the sample adding needle and the sample adding plate is slidably connected inside the slide groove;

[0025] The mounting tube on the No. 2 limiting column passes through the reagent plate and is located below the reagent plate. The mounting tube on the No. 2 limiting column is slidably connected to the reagent plate, and the reagent needle is located in the middle of the mounting tube. The part between the reagent needle and the reagent plate is slidably connected inside the slide groove.

[0026] As a preferred solution of the present application; a cleaning plate of a disc structure is arranged on the inner side of the lower end of the mounting tube, a through hole is arranged in the middle of the cleaning plate; a chamfer is arranged at the opening of the upper end of the through hole; a leakage hole is arranged in the middle of the cleaning plate, one end of the leakage hole is located at the chamfer of the through hole, and the other end is located at the lower end of the cleaning plate, the leakage hole is arranged to be inclined downward, and the lower end of the leakage hole is far away from the lower end opening of the through hole;

[0027] The sample adding needle and the reagent needle both pass through the through hole in the middle of the corresponding cleaning plate.

[0028] As a preferred solution of the present application; a plurality of cylinders are arranged on the upper end of the cleaning plate, the interior of the cylinders is slidably connected to the slide rods, and the slide rods are sealed and slidably connected to the inner wall of the cylinders;

[0029] An annular groove is provided inside the cleaning plate, and the annular groove is connected to the air outlet of the cylinder. A plurality of ventilation grooves are provided inside the cleaning plate, and the upper ends of the ventilation grooves are connected to the annular grooves, and the lower ends of the ventilation grooves are located at the lower end of the cleaning plate, and the ventilation grooves are inclined toward the middle of the cleaning plate.

[0030] As a preferred solution of the present application; the annular groove is not connected to the leakage hole, and a connecting hole is provided between the vent groove and the leakage hole, the connecting hole connects the vent groove and the leakage hole, and the connecting hole is inclined toward the vent groove.

[0031] As a preferred solution of the present application; an annular plate is provided at the upper end of the plurality of slide rods, a spiral groove is provided on the inner wall of the mounting tube, a sliding block is provided on the outer side of the annular plate, and the sliding block is slidably connected to the inside of the spiral groove.

[0032] As a preferred solution of the present application, a ball bearing is arranged at the upper end of the annular plate.

[0033] As a preferred solution of the present application, a cylinder is arranged between the plurality of cylinders, an ultraviolet light belt is arranged on the inner wall of the cylinder, and the ultraviolet light belt wraps the corresponding sample addition needle and reagent needle.

[0034] An unattended fully automatic immunoblotting detection method, which is applicable to the above-mentioned unattended fully automatic immunoblotting detection system; the method comprises:

[0035] S1: First, the staff places the sample to be tested into the sample pool, places the sample to be tested into the sample hole on the sample tray, then places the reagent into the reagent pool, places the reagent solution box into the reagent kit placement slot; finally, places the incubation dish into the reaction dish placement slot on the incubation tray;

[0036] S2: Based on the above S1, the automatic immunoblotting detector is then started. After the instrument is started, the No. 1 rotating column drives the No. 1 limiting column and the sample adding plate to rotate, so that the sample adding needle under the sample adding plate is located under its corresponding cleaning tank. During the process, the sample adding needle moves downward to the inside of the cleaning tank, and then the nozzle sprays the cleaning liquid to clean the sample adding needle;

[0037] S3: Based on the above S2, after cleaning, the sample needle moves out of the cleaning tank; then the No. 1 rotating column rotates, and after the sample needle is moved to the top of the sample pool, the sample needle moves downward to extract the sample to be tested in the sample tube, and then the No. 1 rotating column rotates to rotate the sample needle to the top of the reaction pool, and then the sample needle injects the sample to be tested into the incubation dish; then the incubation dish inside the reaction pool rotates;

[0038] S4: During the above S3 process, the telescopic rod on the second rotating column is retracted downward to send the reagent needle into the cleaning tank for cleaning. After the reagent needle is cleaned, the second rotating column moves the reagent needle to the top of the reagent pool, and then the reagent needle extracts the reagent solution in the reagent kit; after the reagent needle is extracted, the reagent needle injects the reagent solution into the incubation dish;

[0039] S5: On the basis of the above S4, the incubation plate is then rotated, and the sample to be tested in the incubation dish is mixed with the reagent solution during the process. When the incubation plate first passes through the position of the No. 3 rotating column and the waste liquid recovery needle during rotation, the incubation plate does not stop; and when the incubation dish containing the reagent solution and the sample to be tested rotates to the lower end of the arc plate, the ultrasonic vibrator vibrates the reagent solution and the sample to be tested inside the incubation dish, so that the reagent solution and the sample to be tested are fully mixed;

[0040] S6: Based on the above S5, the incubation plate is then continuously rotated until the incubation plate drives the incubation dish to rotate to the position of the third rotating column for the second time, and the third rotating column drives the waste liquid recovery needle to move to the top of the incubation dish through the waste liquid recovery plate, and then the waste liquid recovery needle extracts the solution inside the incubation dish. After the collection, the waste liquid recovery needle injects the extracted solution into the waste liquid recovery hole, and the solution flows into the waste liquid recovery box through the waste liquid recovery hole;

[0041] S7: Based on the above S6, the incubation plate continues to rotate until the incubation dish containing the mixed solution of the extracted reagent solution and the test solution moves to the bottom of the arc plate for the second time, and the interpretation and analysis module at the lower end of the arc plate takes an image of the incubation dish, thereby realizing fully automatic detection, improving detection effect, and avoiding waste of manpower.

[0042] The beneficial effects of the present invention are as follows:

[0043] After the sample needle is cleaned, part of the cleaning liquid will remain on the surface of the sample needle. At this time, the sample needle needs to move upward to reset. During the upward movement of the sample needle, the mounting tube and the cleaning plate are in a stationary state. Therefore, the sample needle, the mounting tube and the cleaning plate are in a relative motion state, so that the sample needle moves upward in the through hole in the middle of the cleaning plate. During the process, the inner wall of the through hole is in close contact with the outer side of the sample needle, so that the cleaning plate can scrape off the cleaning liquid on the surface of the sample needle, avoiding the problem of cross infection when sampling the sample to be tested, thereby improving the accuracy of the detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 is a three-dimensional diagram of the instrument body of the present invention;

[0045] Figure 2 It is a stereogram of the instrument body of the present invention from another viewing angle;

[0046] Figure 3 is a top view of the instrument body of the present invention;

[0047] Figure 4 It is a structural view of the cleaning tank and the waste liquid recovery hole in the present invention;

[0048] Figure 5 It is a structural view of the first rotating column and the second rotating column in the present invention;

[0049] Figure 6 The present invention Figure 5 Cross-sectional view of the sample plate;

[0050] Figure 7 It is a structural view of the sample plate in the present invention;

[0051] Figure 8is a view of the internal structure of the installation tube in the present invention;

[0052] Fig. 9 is a cross-sectional view of the installation pipe in the present invention;

[0053] Fig.10 It is a structural view of the spiral groove in the present invention;

[0054] Fig.11 is a cross-sectional view of a cleaning plate in the present invention;

[0055] Fig.12 is a flow chart of the method in the present invention;

[0056] In the figure: instrument body 1, sample pool 11, sample tray 111, sample hole 112, reagent pool 12, reagent tray 121, reagent kit placement slot 122, reaction pool 13, incubation tray 131, reaction vessel placement slot 132, No. 1 rotating column 14, No. 1 limiting column 141, sample adding plate 142, sample adding needle 143, No. 2 rotating column 15, No. 2 limiting column 151, reagent plate 152, reagent needle 153, No. 3 rotating column 16, waste liquid recovery plate 161, waste liquid recovery needle 162 , waste liquid recovery hole 163, waste liquid recovery box 164, cleaning tank 17, cleaning liquid storage box 171, nozzle 172, arc plate 18, ultrasonic vibrator 181, judgment and analysis module 182, mounting plate 2, mounting tube 21, slide groove 22, cleaning plate 23, through hole 231, chamfer 232, annular groove 233, ventilation groove 234, connecting hole 235, leakage hole 24, cylinder 25, slide rod 26, annular plate 27, ball 271, spiral groove 28, cylinder 29. DETAILED DESCRIPTION

[0057] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0058] Embodiment 1:

[0059] like Figures 1 to 11 As shown; an unattended fully automatic immunoblotting detection system, the monitoring system includes an automatic immunoblotting detector, the automatic immunoblotting detector includes an instrument body 1, the instrument body 1 is provided with a sample pool 11, a reagent pool 12 and a reaction pool 13;

[0060] A sample disk 111 is rotatably connected to the sample pool 11, and a plurality of sample holes 112 are provided on the sample disk 111;

[0061] The reagent pool 12 is rotatably connected to a reagent tray 121, a refrigeration device is provided inside the reagent tray 121, and a plurality of reagent tray placement slots 122 are provided on the reagent tray 121;

[0062] The reaction pool 13 is rotatably connected to an incubation tray 131, a heating device is provided inside the incubation tray 131, and a plurality of reaction vessel placement slots 132 are provided on the incubation tray 131;

[0063] A rotating column 14 is arranged between the sample pool 11 and the reaction pool 13, a limiting column 141 is arranged at the center of the upper end of the rotating column 14, and a telescopic rod is eccentrically arranged at the upper end of the rotating column 14; a sample adding plate 142 is slidably connected to the limiting column 141, the sample adding plate 142 is connected to the output end of the telescopic rod, and a sample adding needle 143 is arranged at the lower end of the sample adding plate 142 away from the limiting column 141;

[0064] A second rotating column 15 is arranged between the reagent pool 12 and the reaction pool 13, a second limiting column 151 is arranged at the center of the upper end of the second rotating column 15, a telescopic rod is eccentrically arranged at the upper end of the second rotating column 15, a reagent plate 152 is slidably connected to the second limiting column 151, and the reagent plate 152 is connected to the output end of the telescopic rod; a reagent needle 153 is arranged at the lower end of the reagent plate 152 away from the second limiting column 151;

[0065] A third rotating column 16 is provided on one side of the reaction pool 13, a telescopic rod is provided on the third rotating column 16, a waste liquid recovery plate 161 is provided on the telescopic rod of the third rotating column 16, and a waste liquid recovery needle 162 is provided at the lower end of the waste liquid recovery plate 161; a waste liquid recovery hole 163 is provided at the upper end of the instrument body 1; a waste liquid recovery box 164 is provided inside the instrument body 1, and the waste liquid recovery box 164 is connected to the waste liquid recovery hole 163 through a hose;

[0066] The upper end of the instrument body 1 is provided with two cleaning grooves 17. In the initial state, one of the cleaning grooves 17 is located directly below the sample injection needle 143, and the other cleaning groove 17 is located directly below the reagent needle 153. A cleaning liquid storage box 171 is provided inside the instrument body 1, and the cleaning liquid storage box 171 is connected to the cleaning groove 17 through a hose. A nozzle 172 is provided inside the cleaning groove 17, and the nozzle 172 is connected to the cleaning liquid storage box 171 through a hose.

[0067] The instrument body 1 is provided with an arc plate 18, a telescopic rod is provided below one side of the arc plate 18, the telescopic rod below the arc plate 18 is provided on the instrument body 1, and an ultrasonic vibrator 181 and a reading and analysis module 182 are provided on the other side of the arc plate 18; the ultrasonic vibrator 181 and the reading and analysis module are located above the incubation tray 131;

[0068] The upper ends of the No. 1 limiting column 141 and the No. 2 limiting column 151 are both provided with mounting plates 2, the mounting plates 2 on the No. 1 limiting column 141 are located directly above the sample adding plate 142, and the mounting plates 2 on the No. 2 limiting column 151 are located directly above the reagent plate 152, and the ends of the two mounting plates 2 away from the telescopic rod are both provided with mounting tubes 21, and the sides of the mounting tubes 21 are provided with slide grooves 22; the mounting tube 21 on the No. 1 limiting column 141 passes through the sample adding plate 142 and is located below the sample adding plate 142, the mounting tube 21 on the No. 1 limiting column 141 is slidably connected with the sample adding plate 142, and the sample adding needle 143 is located in the middle of the mounting tube 21, and the part between the sample adding needle 143 and the sample adding plate 142 is slidably connected inside the slide groove 22;

[0069] The mounting tube 21 on the second limiting column 151 passes through the reagent plate 152 and is located below the reagent plate 152. The mounting tube 21 on the second limiting column 151 is slidably connected to the reagent plate 152, and the reagent needle 153 is located in the middle of the mounting tube 21. The part between the reagent needle 153 and the reagent plate 152 is slidably connected inside the slide groove 22.

[0070] The specific workflow is as follows;

[0071] When using the reagent, the staff first places the sample to be tested into the sample pool 11, places the sample to be tested into the sample hole on the sample tray, then places the reagent into the reagent pool 12, places the reagent liquid box into the reagent box placement slot 122; finally, places the incubation dish into the reaction dish placement slot 132 on the incubation tray 131;

[0072] Then the automatic immunoblotting detector is started. After the instrument is started, the No. 1 rotating column 14, the No. 2 rotating column 15 and the No. 3 rotating column 16 on the instrument body 1 can rotate on the instrument body 1. The rotation method is driven by a stepper motor arranged under the three. When the sample to be tested is tested, the No. 1 rotating column 14 rotates first, and the No. 1 rotating column 14 drives the No. 1 limiting column 141 and the sample adding plate 142 to rotate, so that the sample adding needle 143 under the sample adding plate 142 is located under its corresponding cleaning tank 17. Then the telescopic rod on the No. 1 rotating column 14 is retracted, driving the sample adding plate 142 to rotate. 2 moves downward, during which the sample adding needle 143 moves downward to the inside of the cleaning tank 17, and then the nozzle 172 in the cleaning tank 17 is started, and the nozzle 172 pumps the cleaning liquid in the cleaning liquid storage box 171 into the inside of the nozzle 172 through the pump provided on the instrument body 1, and then the nozzle 172 sprays the cleaning liquid to clean the sample adding needle 143. After cleaning, the telescopic rod on the No. 1 rotating column 14 extends, driving the sample adding plate 142 and the sample adding needle 143 to move upward, and the sample adding needle 143 moves out of the cleaning tank 17; then the No. 1 rotating column 14 rotates, driving the No. 1 limiting column 141 and the sample adding needle 143 to move upward, and the sample adding needle 143 moves out of the cleaning tank 17; The sample plate 142 and the sample adding needle 143 rotate synchronously until the sample adding needle 143 is located directly above the sample pool. At this time, the sample plate inside the sample pool rotates until the sample tube to be tested rotates to directly below the sample adding needle 143. The telescopic rod on the No. 1 rotating column 14 contracts, driving the sample adding plate 142 to move downward on the No. 1 limiting column 141. During the process, the sample adding needle 143 moves downward until the sample adding needle 143 moves to the inside of the sample tube to be tested. Then, the sample adding needle 143 extracts the sample to be tested inside the sample tube (the extraction method is the pneumatic extraction in the prior art, which will not be described in detail here). (It will be repeated) After the sampling is completed, the telescopic rod on the No. 1 rotating column 14 drives the sample adding needle 143 to move upward; then the No. 1 rotating column 14 rotates to rotate the sample adding needle 143 to the top of the reaction pool 13, and then the telescopic rod on the No. 1 rotating column 14 contracts downward to move the sample adding needle 143 to the incubation dish on the incubation disk 131, and then the sample adding needle 143 injects the sample to be tested into the incubation dish; then the incubation disk 131 inside the reaction pool 13 rotates, and the rotation mode of the incubation disk 131 is driven by the driving motor arranged at the lower end of the incubation disk 131, and during the rotation of the incubation disk 131;

[0073] The second rotating column 15 rotates, and when the second rotating column 15 drives the second limiting column 151, the reagent disk 121 and the reagent needle 153 to the top of the cleaning groove 17 corresponding to the rotating column, the telescopic rod on the second rotating column 15 shrinks downward to send the reagent needle 153 into the cleaning groove 17, and then the nozzle 172 in the cleaning groove 17 corresponding to the reagent needle 153 cleans the reagent needle 153 (the cleaning method and cleaning principle of the reagent needle 153 are the same as the cleaning method of the above-mentioned sample addition needle 143); after the cleaning of the reagent needle 153 is completed, the telescopic rod on the second rotating column 15 extends to drive the reagent needle 153 to reset, and after the reagent needle 153 is reset, the second rotating column 15 drives it to rotate until the reagent needle 153 moves to the top of the reagent pool 12, and then the reagent disk 121 inside the reagent pool 12 rotates. During the rotation of the reagent disk 121, the refrigeration device arranged inside the reagent pool 12 cools the reagent liquid. , so that the temperature of the reagent solution is maintained between 0-6°C, until the required reagent solution box rotates to the bottom of the reagent needle 153, the telescopic rod on the No. 2 rotating column 15 contracts, and the reagent needle 153 is sent into the reagent solution box, and then the reagent needle 153 extracts the reagent solution in the reagent kit (the extraction method is the same as the extraction method of the sample injection needle 143); after the reagent needle 153 is extracted, the telescopic rod on the No. 2 rotating column 15 extends and resets, driving the reagent needle 153 to reset, and then the No. 2 rotating column 15 rotates to rotate the reagent needle 153 to the top of the reaction pool 13; in this process, the incubation disk 131 rotates until the incubation dish where the sample injection needle 143 is injected into the sample to be tested rotates to the bottom of the reagent needle 153, the telescopic rod on the No. 2 rotating column drives the reagent needle 153 to move downward, and sends the reagent needle 153 to the top of the incubation dish, and then the reagent needle 153 injects the reagent solution into the incubation dish;

[0074] Then the incubation plate 131 rotates, and during the rotation, the heating device arranged inside the reaction pool 13 heats the incubation dish (the heating device is a prior art and will not be described in detail here); during the rotation of the incubation plate 131, the sample to be tested in the incubation dish is mixed with the reagent solution, and when the incubation plate 131 passes through the position of the third rotating column 16 and the waste liquid recovery needle 162 for the first time during rotation, the incubation plate 131 does not stop; and when the incubation dish containing the reagent solution and the sample to be tested rotates to the lower end of the arc plate 18, the arc plate The telescopic rod at the lower end of 18 drives the arc plate 18 to move downward, and the ultrasonic vibrator 181 at the lower end of the arc plate 18 vibrates the reagent solution and the sample to be tested in the incubation dish, so that the reagent solution and the sample to be tested are fully mixed, and then the incubation plate 131 continues to rotate until the incubation dish 131 drives the incubation dish to rotate to the position of the third rotating column 16 for the second time, and the third rotating column 16 drives the waste liquid recovery needle 162 to move to the top of the incubation dish through the waste liquid recovery plate 161, and then the telescopic rod above the third rotating column 16 is retracted. The waste liquid recovery needle 162 is retracted to transport it to the inside of the incubation dish, and then the waste liquid recovery needle 162 extracts the solution inside the incubation dish. After the collection, the telescopic rod on the No. 3 rotating column 16 is extended, and then the No. 3 rotating column 16 rotates the waste liquid recovery needle 162 to the top of the waste liquid recovery hole 163, and then the telescopic rod on the No. 3 rotating column 16 transports the waste liquid recovery needle 162 into the waste liquid recovery hole 163, and then the solution extracted by the waste liquid recovery needle 162 is injected into the waste liquid recovery hole 163, and the solution passes through the waste liquid recovery hole 163 flows into the interior of the waste liquid recovery box 164, and then the incubation plate 131 continues to rotate until the incubation dish containing the mixed solution of the extracted reagent liquid and the test liquid moves to the bottom of the arc plate 18 for the second time, and the judgment and analysis module 182 at the lower end of the arc plate 18 takes an image of the incubation dish (the judgment and analysis module 182 is a camera, which analyzes the taken image and outputs the result, and transmits the output result to the display on the instrument body 1); thereby achieving full-automatic detection, improving detection effect, and avoiding waste of manpower;

[0075] In this process, the sample needle 143 and the reagent needle 153 are used to extract the sample or reagent solution to be tested; through the installation of the installation tube 21 and the installation plate 2; the following effects are achieved; since the reagent needle 153 and the installation tube 21 installed outside the sample needle 143 have the same effect and principle, the sample needle 143 is taken as an example here;

[0076] A mounting plate 2 is provided at the upper end of the No. 1 limiting column 141, and the mounting plate 2 is located directly above the sample adding plate 142, and the mounting plate 2 and the sample adding plate 142 are in a state of synchronous movement; in the process of the sample adding needle 143 sampling the sample to be tested inside the sample pool, when the sample adding needle 143 moves to the top of the sample pool, the telescopic rod on the No. 1 rotating column 14 contracts, and the sample adding moves downward. In the process, the sample adding plate 142 moves downward on the No. 1 limiting column 141, but the mounting plate 2 and the No. 1 limiting column 141 are fixedly connected, so the mounting plate 2 will not move downward, so that the mounting tube 21 will not move downward, because the lower end of the mounting tube 21 passes through the sample adding plate 142 and is slidably connected to the sample adding plate 142, and the side wall of the mounting tube 21 is provided with a slide groove 22, so that a partial area between the sample adding needle 143 and the sample adding plate 142 is slidably connected to the slide The inside of the groove 22 ensures that the sample adding needle 143 will not be suspended. During the downward movement of the sample adding needle 143 and the sample adding plate 142, the area between the sample adding needle 143 and the sample adding plate 142 moves downward inside the slide groove 22. During the process, the lower end of the sample adding needle 143 will gradually extend out of the mounting tube 21, allowing the sample adding needle 143 to extract the sample to be tested; after the extraction is completed, the sample adding needle 143 rises, and the sample adding plate 142 rises on the No. 1 limiting column 141, but the mounting tube 21 does not move, allowing the sample adding needle 143 to enter the inside of the mounting tube 21. Since the sample adding needle 143 is in the non-working state, the sample adding needle 143 is in the temporal part of the mounting tube 21, the mounting tube 21 protects the sample adding needle 143 to avoid the problem of people accidentally touching the sample adding needle 143; thereby improving the safety of the sample adding needle 143 and avoiding damage to it.

[0077] Embodiment 2:

[0078] like Figures 2 to 11 As shown; a cleaning plate 23 of a disc structure is arranged on the inner side of the lower end of the mounting tube 21, and a through hole 231 is opened in the middle of the cleaning plate 23; a chamfer 232 is opened at the opening of the upper end of the through hole 231; a leakage hole 24 is opened in the middle of the cleaning plate 23, one end of the leakage hole 24 is located at the chamfer 232 of the through hole 231, and the other end is located at the lower end of the cleaning plate 23, and the leakage hole 24 is arranged to be inclined downward, and the lower end of the leakage hole 24 is away from the lower end opening of the through hole 231;

[0079] The sample injection needle 143 and the reagent needle 153 both pass through the through hole 231 in the middle of the corresponding cleaning plate 23;

[0080] The specific workflow is as follows;

[0081] A cleaning plate 23 with a disc structure is provided on the inner side of the lower end of the mounting tube 21, and a through hole 231 is provided in the middle of the cleaning plate 23, so that the sample needle 143 and the reagent needle 153 extend from the corresponding through hole 231; based on the above embodiment, after the sample needle 143 is cleaned, part of the cleaning liquid will remain on the surface of the sample needle 143. At this time, since the sample needle 143 needs to move upward to reset after the cleaning is completed, during the upward movement of the sample needle 143 , the mounting tube 21 and the cleaning plate 23 are in a stationary state, so the sample needle 143 is in a relative motion state with the mounting tube 21 and the cleaning plate 23, so that the sample needle 143 moves upward in the through hole 231 in the middle of the cleaning plate 23. During the process, the inner wall of the through hole 231 is in close contact with the outer side of the sample needle 143, so that the cleaning plate 23 scrapes off the cleaning liquid on the surface of the sample needle 143, thereby avoiding the problem of cross infection when the sample needle 143 samples the sample to be tested, thereby improving the accuracy of the detection;

[0082] A chamfer 232 is provided on the inner side of the upper end of the through hole 231, and a leakage hole 24 is provided inside the cleaning plate 23. When the sample adding needle 143 contacts the through hole 231, an annular groove is generated between the sample adding needle 143 and the chamfer 232 on the through hole 231. Since the sample adding needle 143 is in a frequent working state, if the liquid on its surface is not completely cleaned during the upward movement of the sample adding needle 143 and enters the top of the cleaning plate 23, when the sample adding needle 143 moves downward, the cleaning plate 23 moves upward on the surface of the sample adding needle 143. At this time, the liquid of the sample adding needle 143 located above the cleaning plate 23 will gather at the chamfer 232, and then can leak out from the cleaning plate 23 through the leakage hole 24, thereby improving the cleaning effect of the sample adding needle 143, further avoiding cross infection, and improving the detection effect.

[0083] Embodiment three:

[0084] like Figures 2 to 10 As shown; a plurality of cylinders 25 are provided at the upper end of the cleaning plate 23, the inner part of the cylinder 25 is slidably connected to the slide rod 26, and the slide rod 26 is sealed and slidably connected to the inner wall of the cylinder 25;

[0085] An annular groove 233 is provided inside the cleaning plate 23, and the annular groove 233 is communicated with the air outlet of the cylinder 25. A plurality of ventilation grooves 234 are provided inside the cleaning plate 23, and the upper ends of the ventilation grooves 234 are communicated with the annular groove 233, and the lower ends of the ventilation grooves 234 are located at the lower end of the cleaning plate 23, and the ventilation grooves 234 are inclined toward the middle of the cleaning plate 23;

[0086] The annular groove 233 is not connected to the leakage hole 24, and a connecting hole 235 is provided between the vent groove 234 and the leakage hole 24. The connecting hole 235 connects the vent groove 234 and the leakage hole 24, and the connecting hole 231 is inclined toward the vent groove 234.

[0087] The specific workflow is as follows;

[0088] A plurality of cylinders 25 are arranged at the upper end of the cleaning plate 23, and the interior of the cylinder 25 is slidably connected to a connecting rod, and the connecting rod is sealed and slidably connected to the inner wall of the cylinder 25, so that when the sample needle 143 is cleaned, the sample needle 143 moves upward, and after the cleaning plate 23 and the through hole 231 perform the initial cleaning on its surface, when the sample needle 143 takes samples of the sample to be tested, the sample needle 143 moves downward, and the sample plate 142 moves downward during the process, the mounting tube 21 and the cleaning plate 23 remain stationary, and the portion of the sample plate 142 located inside the mounting tube 21 pushes the upper end of the slide bar 26, so that the slide bar 26 moves downward in the cylinder 25, squeezing the gas in the cylinder 25; at this time, the gas in the cylinder 25 The gas will enter the annular groove 233 provided in the cleaning plate 23. Since the cleaning plate 23 is also provided with a ventilation groove 234, which is connected to the annular groove 233, the gas in the annular groove 233 will enter the ventilation groove 234, and the gas will be ejected from the ventilation groove 234. Since the ventilation groove 234 is inclined toward the middle of the cleaning plate 23, and the sample needle 143 passes through the middle of the cleaning plate 23, when the sample needle 143 moves downward to take a sample, the gas ejected from the ventilation groove 234 will blow on the surface of the sample needle 143, thereby preventing the surface of the sample needle 143 from being in a wet state and preventing dust from appearing on the surface of the sample needle 143, which will affect the detection accuracy.

[0089] The annular groove 233 is not connected to the leakage hole 24, so that the gas inside the annular groove 233 will not enter the leakage hole 24, and the ventilation groove 234 is connected to the leakage hole 24 through the connecting hole 235 opened in the cleaning plate 23, and the connecting hole 235 is inclined downward, and the connecting hole 235 is inclined toward the ventilation groove 234. When the gas inside the annular groove 233 is ejected from the ventilation groove 234, the gas moves inside the ventilation groove 234 and passes through the connecting hole 235. Due to the flow of gas, the gas flow rate inside the ventilation groove 234 is large, which will attract the gas inside the leakage hole 24, so that the gas inside the leakage hole 24 enters the ventilation groove 234, and then drives the gas inside the leakage hole 24 to circulate, so as to avoid the leakage hole 24 being in a humid state for a long time and the air is not circulating, so as to avoid the problem of easy breeding of bacteria inside the leakage hole 24, further improve the cleaning effect of the sampling needle 143, and then improve the detection accuracy.

[0090] Embodiment 4:

[0091] like Figures 1 to 11 As shown; an annular plate 27 is provided at the upper end of the plurality of slide rods 26, a spiral groove 28 is provided on the inner wall of the mounting tube 21, a slider is provided on the outer side of the annular plate 27, and the slider is slidably connected to the inside of the spiral groove 28;

[0092] The upper end of the annular plate 27 is provided with a ball 271;

[0093] A cylinder 29 is disposed between the plurality of cylinders 25, and an ultraviolet light belt is disposed on the inner wall of the cylinder 29, and the ultraviolet light belt wraps the corresponding sample injection needle 143 and reagent needle 153;

[0094] The specific workflow is as follows;

[0095] An annular plate 27 is provided at the upper end of the slide bar 26, and a spiral groove 28 is provided on the inner wall of the mounting tube 21, and a slider is provided on the outer side of the annular plate 27, and the slider is slidably connected to the inside of the spiral groove 28; so that during the downward movement of the sample adding needle 143, one end of the sample adding plate 142 located inside the mounting tube 21 presses the annular plate 27, and the annular plate 27 pushes the slide bar 26 to move downward inside the cylinder 25. During the downward movement of the annular plate 27, since the outer side of the annular plate 27 is slidably connected to the inside of the spiral groove 28 through the slider, The slider is guided by the spiral groove 28 to rotate, thereby driving the annular plate 27 to rotate. The annular plate 27 drives the cylinder 25 to rotate through the slide rod 26. The cylinder 25 is fixedly connected to the cleaning plate 23. The cleaning plate 23 is slidably connected to the inner wall of the mounting tube 21. Therefore, the cleaning plate 23 rotates with the cleaning plate 23. When the cleaning plate 23 rotates, it rotates and wipes the surface of the sample adding needle 143, thereby improving the cleaning effect of the surface of the sample adding needle 143, further avoiding the problem of cross infection of the sample adding needle 143, and further improving the detection accuracy.

[0096] A ball 271 is arranged on the annular plate 27 to reduce the friction between the annular plate 27 and the sample adding plate 142 during rotation, thereby reducing the resistance to annular rotation and improving the service life. A cylinder 29 is arranged between the plurality of cylinders 25, and an ultraviolet light strip is arranged on the inner wall of the cylinder 29, so that the ultraviolet light and the like can wrap around the sample adding needle 143 and the reagent needle 153, so that in the process of cleaning the surface of the sample adding needle 143 or the reagent needle 153, the ultraviolet light strip is always irradiating and sterilizing the surface of the sample adding needle 143 or the reagent needle 153, thereby further improving the cleaning effect of the surface of the sample adding needle 143 and the reagent needle 153, thereby avoiding cross contamination and improving the detection accuracy.

[0097] Embodiment five:

[0098] like Fig.12As shown; an unattended fully automatic immunoblotting detection method, the method is applicable to the above unattended fully automatic immunoblotting detection system; the method comprises:

[0099] S1: First, the staff places the sample to be tested into the sample pool 11, places the sample to be tested into the sample hole on the sample tray, then places the reagent into the reagent pool 12, places the reagent solution box into the reagent kit placement slot 122; finally, places the incubation dish into the reaction dish placement slot 132 on the incubation tray 131;

[0100] S2: Based on the above S1, the automatic immunoblotting detector is then started. After the instrument is started, the first rotating column 14 drives the first limiting column 141 and the sample adding plate 142 to rotate, so that the sample adding needle 143 under the sample adding plate 142 is located under its corresponding cleaning tank 17. During the process, the sample adding needle 143 moves downward to the inside of the cleaning tank 17, and then the nozzle 172 sprays the cleaning liquid to clean the sample adding needle 143;

[0101] S3: Based on the above S2, after cleaning, the sample adding needle 143 moves out of the cleaning tank 17; then the No. 1 rotating column 14 rotates, and after the sample adding needle 143 is moved to the top of the sample pool, the sample adding needle 143 moves downward to extract the sample to be tested in the sample tube, and then the No. 1 rotating column 14 rotates to rotate the sample adding needle 143 to the top of the reaction pool 13, and then the sample adding needle 143 injects the sample to be tested into the incubation dish; then the incubation plate 131 in the reaction pool 13 rotates;

[0102] S4: During the above S3 process, the telescopic rod on the second rotating column 15 is retracted downward, and the reagent needle 153 is sent to the cleaning tank 17 for cleaning. After the reagent needle 153 is cleaned, the second rotating column 15 moves the reagent needle 153 to the top of the reagent pool 12, and then the reagent needle 153 extracts the reagent solution in the reagent kit; after the reagent needle 153 extracts the reagent solution, the reagent needle 153 injects the reagent solution into the incubation dish;

[0103] S5: On the basis of the above S4, the incubation plate 131 then rotates, and the sample to be tested in the incubation dish is mixed with the reagent solution during the process. When the incubation plate 131 first passes through the position of the third rotating column 16 and the waste liquid recovery needle 162 during the rotation, the incubation plate 131 does not stop; and when the incubation dish containing the reagent solution and the sample to be tested rotates to the lower end of the arc plate 18, the ultrasonic vibrator 181 vibrates the reagent solution and the sample to be tested inside the incubation dish, so that the reagent solution and the sample to be tested are fully mixed;

[0104] S6: On the basis of the above S5, the incubation tray 131 then continues to rotate until the incubation tray 131 drives the incubation dish to rotate to the position of the third rotating column 16 for the second time, and the third rotating column 16 drives the waste liquid recovery needle 162 to move to the top of the incubation dish through the waste liquid recovery plate 161, and then the waste liquid recovery needle 162 extracts the solution inside the incubation dish. After the collection, the waste liquid recovery needle 162 injects the extracted solution into the waste liquid recovery hole 163, and the solution flows into the waste liquid recovery box 164 through the waste liquid recovery hole 163;

[0105] S7: Based on the above S6, the incubation plate 131 continues to rotate until the incubation dish containing the mixed solution of the extracted reagent solution and the test solution moves to the bottom of the arc plate 18 for the second time. The interpretation and analysis module 182 at the lower end of the arc plate 18 takes an image of the incubation dish, thereby realizing fully automatic detection, improving detection effect, and avoiding waste of manpower.

Claims

1. An unattended fully automatic immunoblot detection system, the monitoring system comprising an automatic immunoblot detector, the automatic immunoblot detector comprising an instrument body (1), characterized in that: The instrument body (1) is provided with a sample pool (11), a reagent pool (12) and a reaction pool (13); A sample disk (111) is rotatably connected inside the sample pool (11), and a plurality of sample holes (112) are provided on the sample disk (111); The reagent pool (12) is rotatably connected to a reagent disk (121), a refrigeration device is provided inside the reagent disk (121), and a plurality of reagent kit placement slots (122) are provided on the reagent disk (121); The reaction pool (13) is rotatably connected to an incubation tray (131), a heating device is provided inside the incubation tray (131), and a plurality of reaction vessel placement slots (132) are provided on the incubation tray (131); A No. 1 rotating column (14) is arranged between the sample pool (11) and the reaction pool (13); a No. 1 limiting column (141) is arranged at the center of the upper end of the No. 1 rotating column (14); a telescopic rod is eccentrically arranged at the upper end of the No. 1 rotating column (14); a sample adding plate (142) is slidably connected to the No. 1 limiting column (141); the sample adding plate (142) is connected to the output end of the telescopic rod; a sample adding needle (143) is arranged at the lower end of the sample adding plate (142) on a side away from the No. 1 limiting column (141); A second rotating column (15) is arranged between the reagent pool (12) and the reaction pool (13); a second limiting column (151) is arranged at the center of the upper end of the second rotating column (15); a telescopic rod is eccentrically arranged at the upper end of the second rotating column (15); a reagent plate (152) is slidably connected to the second limiting column (151); the reagent plate (152) is connected to the output end of the telescopic rod; a reagent needle (153) is arranged at the lower end of the reagent plate (152) away from the second limiting column (151); A third rotating column (16) is arranged on one side of the reaction pool (13), a telescopic rod is arranged on the third rotating column (16), a waste liquid recovery plate (161) is arranged on the telescopic rod of the third rotating column (16), and a waste liquid recovery needle (162) is arranged at the lower end of the waste liquid recovery plate (161); a waste liquid recovery hole (163) is opened at the upper end of the instrument body (1); a waste liquid recovery box (164) is arranged inside the instrument body (1), and the waste liquid recovery box (164) is connected to the waste liquid recovery hole (163) through a hose; The upper end of the instrument body (1) is provided with two cleaning grooves (17). In an initial state, one of the cleaning grooves (17) is located directly below the sample injection needle (143), and the other cleaning groove (17) is located directly below the reagent needle (153). A cleaning liquid storage box (171) is provided inside the instrument body (1), and the cleaning liquid storage box (171) is connected to the cleaning groove (17) through a hose. A nozzle (172) is provided inside the cleaning groove (17), and the nozzle (172) is connected to the cleaning liquid storage box (171) through a hose. The instrument body (1) is provided with an arc-shaped plate (18), a telescopic rod is provided below one side of the arc-shaped plate (18), the telescopic rod below the arc-shaped plate (18) is provided on the instrument body (1), and an ultrasonic vibrator (181) and a reading and analysis module (182) are provided on the other side of the arc-shaped plate (18); the ultrasonic vibrator (181) and the reading and analysis module are located above the incubation tray (131).

2. An unattended fully automatic immunoblotting detection system as claimed in claim 1, characterized in that: The upper ends of the first limiting column (141) and the second limiting column (151) are both provided with mounting plates (2); the mounting plate (2) on the first limiting column (141) is located directly above the sample adding plate (142); the mounting plate (2) on the second limiting column (151) is located directly above the reagent plate (152); the ends of the two mounting plates (2) away from the telescopic rod are both provided with mounting tubes (21); and the side surfaces of the mounting tubes (21) are provided with mounting tubes (21). A slide groove (22) is provided; the mounting tube (21) on the first limiting column (141) passes through the sample adding plate (142) and is located below the sample adding plate (142); the mounting tube (21) on the first limiting column (141) is slidably connected to the sample adding plate (142); the sample adding needle (143) is located in the middle of the mounting tube (21); and the portion between the sample adding needle (143) and the sample adding plate (142) is slidably connected inside the slide groove (22); The mounting tube (21) on the second limiting column (151) passes through the reagent plate (152) and is located below the reagent plate (152); the mounting tube (21) on the second limiting column (151) is slidably connected to the reagent plate (152); the reagent needle (153) is located in the middle of the mounting tube (21); and the portion between the reagent needle (153) and the reagent plate (152) is slidably connected inside the slide groove (22).

3. An unattended fully automatic immunoblotting detection system as claimed in claim 2, characterized in that: A cleaning plate (23) with a disc structure is arranged on the inner side of the lower end of the mounting tube (21), and a through hole (231) is provided in the middle of the cleaning plate (23); a chamfer (232) is provided at the opening of the upper end of the through hole (231); a liquid leakage hole (24) is provided in the middle of the cleaning plate (23), one end of the liquid leakage hole (24) is located at the chamfer (232) of the through hole (231), and the other end is located at the lower end of the cleaning plate (23); the liquid leakage hole (24) is arranged to be inclined downward, and the lower end of the liquid leakage hole (24) is away from the lower end opening of the through hole (231); The sample adding needle (143) and the reagent needle (153) both pass through the through hole (231) in the middle of the corresponding cleaning plate (23).

4. An unattended fully automatic immunoblotting detection system as claimed in claim 3, characterized in that: A plurality of cylinders (25) are arranged at the upper end of the cleaning plate (23), the interior of the cylinders (25) is slidably connected to a slide rod (26), and the slide rod (26) is sealed and slidably connected to the inner wall of the cylinder (25); An annular groove (233) is provided inside the cleaning plate (23), and the annular groove (233) is communicated with the air outlet of the cylinder (25). A plurality of ventilation grooves (234) are provided inside the cleaning plate (23), and the upper ends of the ventilation grooves (234) are communicated with the annular groove (233), and the lower ends of the ventilation grooves (234) are located at the lower end of the cleaning plate (23), and the ventilation grooves (234) are inclined toward the middle of the cleaning plate (23).

5. An unattended fully automatic immunoblotting detection system as claimed in claim 4, characterized in that: The annular groove (233) is not connected to the liquid leakage hole (24); a connecting hole (235) is provided between the vent groove (234) and the liquid leakage hole (24); the connecting hole (235) enables the vent groove (234) and the liquid leakage hole (24) to be connected; and the connecting hole (231) is inclined toward the vent groove (234).

6. An unattended fully automatic immunoblotting detection system as claimed in claim 4, characterized in that: An annular plate (27) is provided at the upper end of the plurality of slide rods (26), a spiral groove (28) is provided on the inner wall of the mounting tube (21), and a sliding block is provided on the outer side of the annular plate (27), and the sliding block is slidably connected inside the spiral groove (28).

7. An unattended fully automatic immunoblotting detection system as claimed in claim 6, characterized in that: A ball (271) is disposed at the upper end of the annular plate (27).

8. An unattended fully automatic immunoblotting detection system as claimed in claim 4, characterized in that: A cylinder (29) is arranged between the plurality of cylinders (25), and an ultraviolet light belt is arranged on the inner wall of the cylinder (29), and the ultraviolet light belt wraps the corresponding sample addition needle (143) and reagent needle (153).

9. An unattended fully automatic immunoblotting detection method, which is applicable to the unattended fully automatic immunoblotting detection system of any one of claims 1 to 8 above; characterized in that: The method includes: S1: First, the staff places the sample to be tested into the sample pool (11), places the sample to be tested into the sample hole on the sample tray, then places the reagent into the reagent pool (12), places the reagent solution box into the reagent kit placement slot (122); finally, places the incubation dish into the reaction dish placement slot (132) on the incubation tray (131); S2: Based on the above S1, the automatic immunoblotting detector is then started. After the instrument is started, the first rotating column (14) drives the first limiting column (141) and the sample adding plate (142) to rotate, so that the sample adding needle (143) below the sample adding plate (142) is located below its corresponding cleaning tank (17). During the process, the sample adding needle (143) moves downward to the inside of the cleaning tank (17), and then the nozzle (172) sprays a cleaning liquid to clean the sample adding needle (143); S3: Based on the above S2, after cleaning, the sample adding needle (143) moves out of the cleaning tank (17); then the No. 1 rotating column (14) rotates, and after the sample adding needle (143) is moved to the top of the sample pool, the sample adding needle (143) moves downward to extract the sample to be tested inside the sample tube, and then the No. 1 rotating column (14) rotates to rotate the sample adding needle (143) to the top of the reaction pool (13), and then the sample adding needle (143) injects the sample to be tested into the incubation dish; then the incubation plate (131) inside the reaction pool (13) rotates; S4: During the above S3 process, the telescopic rod on the second rotating column (15) is retracted downward, and the reagent needle (153) is sent to the cleaning tank (17) for cleaning. After the reagent needle (153) is cleaned, the second rotating column (15) moves the reagent needle (153) to the top of the reagent tank (12), and then the reagent needle (153) extracts the reagent solution in the reagent kit; after the reagent needle (153) extracts the reagent solution, the reagent needle (153) injects the reagent solution into the incubation dish; S5: Based on the above S4, the incubation plate (131) is then rotated, and the sample to be tested in the incubation dish is mixed with the reagent solution during the process. When the incubation plate (131) first passes through the position of the third rotating column (16) and the waste liquid recovery needle (162) during the rotation, the incubation plate (131) does not stop; and when the incubation dish containing the reagent solution and the sample to be tested rotates to the lower end of the arc plate (18), the ultrasonic vibrator (181) vibrates the reagent solution and the sample to be tested inside the incubation dish, so that the reagent solution and the sample to be tested are fully mixed; S6: Based on the above S5, the incubation plate (131) is then continuously rotated until the incubation plate (131) drives the incubation dish to rotate twice to the position of the third rotating column (16), and the third rotating column (16) drives the waste liquid recovery needle (162) to move to the top of the incubation dish through the waste liquid recovery plate (161), and then the waste liquid recovery needle (162) extracts the solution inside the incubation dish. After the collection, the waste liquid recovery needle (162) injects the extracted solution into the waste liquid recovery hole (163), and the solution flows into the waste liquid recovery box (164) through the waste liquid recovery hole (163); S7: Based on the above S6, the incubation plate (131) is then continuously rotated until the incubation dish containing the mixed solution of the extracted reagent solution and the test solution moves to the bottom of the arc plate (18) for the second time, and the interpretation and analysis module (182) at the lower end of the arc plate (18) takes an image of the incubation dish, thereby achieving fully automatic detection, improving detection results, and avoiding waste of manpower.

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