Agar gel plate puncher

Abstract

The invention relates to an agar gel plate puncher, and relates to the field of agar gel punching devices.The agar gel plate puncher comprises a negative pressure generation assembly, a punching pipe, a switch control assembly and a display assembly, a negative pressure space can be generated in the negative pressure generation assembly, the negative pressure space communicates with the punching pipe, and the switch control assembly is connected with the display assembly; the negative pressure generating assembly is arranged between the negative pressure space and the punching pipe so that agar blocks can be sucked into the punching pipe, the switch control assembly is arranged between the negative pressure generating assembly and the punching pipe so that connection and disconnection of a pipeline between the negative pressure space and the punching pipe can be controlled, and the display assembly is communicated with the negative pressure space and can display the pressure state of the negative pressure space. According to the device, continuity of punching and agar block taking-out steps is achieved, a needle is not needed to take out the agar block, damage to the hole edge is effectively avoided, residues of the agar block are reduced, influences on experimental results are reduced, and the accuracy of the experimental results is improved.

Description

Technical Field

[0001] The present application relates to the field of agar gel punching devices, and in particular to an agar gel plate puncher. Background Art

[0002] In immunological testing technology, gel precipitation reaction and immunoelectrophoresis are commonly used experimental methods in clinical medical testing and biology. Because the method is simple, the results are intuitive, and no special instruments and equipment are required, it is widely used in the detection of biological samples. The puncher is an indispensable tool in immunological testing. In the test, a series of circular holes of different numbers and diameters need to be punched on the agar plate with the puncher, and then the biological sample is added into it.

[0003] The existing method is manual punching, that is, using a puncher to punch a small hole on the agar plate, and then using a needle or other tools to remove the agar block in the hole. The disadvantage of this punching method is that the punching and the removal of agar cannot be completed in one step, the operation is cumbersome, the efficiency is low, especially when doing a large number of samples, and it is easy to damage the edge of the hole or residual agar block, affecting the experimental results. Summary of the invention

[0004] In order to improve the problem that when agar blocks are taken out with a needle during manual punching, the hole edge may be easily damaged or agar blocks may remain, thus affecting the experimental results, the present application provides an agar gel plate puncher.

[0005] The agar gel plate puncher provided in the present application adopts the following technical solution: An agar gel plate puncher comprises a negative pressure generating component, a punching tube, a switch control component and a display component. The negative pressure generating component can generate a negative pressure space inside, and the negative pressure space is connected to the punching tube so that agar blocks can be sucked into the punching tube. The switch control component is arranged between the negative pressure generating component and the punching tube so as to control the on-off of the pipeline between the negative pressure space and the punching tube. The display component is connected to the negative pressure space and can display the pressure state of the negative pressure space.

[0006] By adopting the above technical scheme, when punching holes in the agar plate, negative pressure is first generated in the negative pressure space by the negative pressure generating component, and then the punching tube is inserted into the agar plate to perform the punching operation, and then the switch control component is used to control the negative pressure space to be connected with the punching tube, and the agar block generated after the punching is sucked into the punching tube under the action of negative pressure. When the agar block moves a distance greater than the length of the agar block in the punching tube, the switch control component controls the negative pressure space to be disconnected from the punching tube, and the agar block stays in the punching tube at this time, and then the punching tube performs the next punching operation, and then the switch control component controls the connection and disconnection of the negative pressure space and the punching tube, and the agar block is sucked into the punching tube, so as to achieve the continuity of the punching and agar block removal steps, and there is no need to use a needle to remove the agar block, which effectively avoids damaging the hole edge, reduces the residue of the agar block, reduces the influence on the experimental results, and improves the accuracy of the experimental results; with the adsorption of the agar block, the display component intuitively reflects the change of the negative pressure in the negative pressure space, which is convenient for people to control the adsorption of the agar block.

[0007] In a specific possible implementation mode, the negative pressure generating component includes a syringe tube, a piston and a piston handle. The piston is slidably disposed in the syringe tube to form the negative pressure space in the syringe tube. The end of the syringe tube forms an air outlet pipe connected to the negative pressure space. The piston handle is fixedly disposed on a side of the piston facing away from the negative pressure space. The syringe tube is provided with a fixing member for limiting the movement of the piston under the action of the negative pressure space.

[0008] By adopting the above technical solution, the piston is pulled to move by the piston handle to generate a negative pressure space in the syringe tube, which has a simple structure and is easy to operate. Then, the position of the piston is fixed by a fixing part to improve the stability of the negative pressure space.

[0009] In a specific possible implementation scheme, the fixing member includes a clamping plate arranged on the syringe tube, and the piston handle is provided with a clamping groove cooperating with the clamping plate. Rotating the clamping plate can make the clamping plate inserted into the clamping groove to fix the clamping plate and the piston handle.

[0010] By adopting the above technical solution, when the piston handle is pulled to move and generate a negative pressure space, the piston handle drives the slot to move to a position relative to the card plate, and then the piston handle is rotated so that the card plate is inserted into the slot, thereby completing the fixation of the piston handle and simplifying the piston handle fixing process.

[0011] In a specific possible implementation scheme, the fixing member includes a spring clip and a fixing part, the fixing part is arranged on the spring clip, the spring clip is fixed to the piston, a fixing hole for inserting the fixing part is opened on the syringe tube, and the spring clip can push the fixing part to insert into the fixing hole to fix the piston to the syringe tube.

[0012] By adopting the above technical solution, when the piston handle is pulled to move to generate a negative pressure space, the fixing part slides to a position relative to the fixing hole as the piston moves, and the spring clip pushes the fixing part to be inserted into the fixing hole to complete the fixing of the piston.

[0013] In a specific possible implementation scheme, the fixing member includes a fixing rod and a fixing spring. The fixing rod is slidably arranged on the syringe tube toward the piston handle. A positioning hole for inserting the fixing rod is formed on the piston handle. The fixing spring is connected to the syringe tube and the fixing rod so as to be able to drive the fixing rod to be inserted into the positioning hole.

[0014] By adopting the above technical solution, when the piston handle is pulled to move and a negative pressure space is generated, the piston handle drives the positioning hole to slide. When the positioning hole slides to a position relative to the fixing rod, the fixing spring pushes the fixing rod to insert into the positioning hole, thereby fixing the piston handle.

[0015] In a specific possible implementation, the switch control component includes a rubber tube and a hard ball. The rubber tube is connected between the air outlet pipe and the perforated tube. The hard ball is arranged in the rubber tube and is used to control the opening and closing of the rubber tube. The hard ball can squeeze the rubber tube to bulge outward and press the rubber tube onto the hard ball to open the rubber tube.

[0016] By adopting the above technical scheme, when connecting the negative pressure space with the perforated tube, the operator presses the rubber tube against the hard ball, causing the contact part of the rubber tube and the hard ball to deform and produce an opening, thereby realizing the connection between the negative pressure space and the perforated tube; when isolating the negative pressure space from the perforated tube, the rubber tube is loosened, and the rubber tube returns to a state of close fit with the hard ball, thereby realizing the isolation of the negative pressure space from the perforated tube, thereby improving the sensitivity of the on-off control of the negative pressure space and the perforated tube.

[0017] In a specific possible implementation scheme, the display assembly includes a balloon, and the balloon is connected to the air outlet pipe and the rubber tube through a three-way pipe joint.

[0018] By adopting the above technical solution, since the balloon is connected to the negative pressure space through the three-way pipe joint, as the negative pressure in the negative pressure space decreases, the balloon will gradually swell from being deflated, playing a role in monitoring the negative pressure, and reflecting the negative pressure more intuitively.

[0019] In a specific implementation scheme, the three-way pipe joint is connected to the rubber tube and an intermediate negative pressure pipe is provided. The intermediate negative pressure pipe and the three-way pipe joint are connected by a control valve. The control valve is used to control the connection and disconnection of the intermediate negative pressure pipe and the perforated tube.

[0020] By adopting the above technical solution, initially, the control valve is opened, the middle negative pressure tube is connected to the negative pressure space and the pressure is the same. When adsorbing the agar block, the control valve is closed, and the negative pressure generated by the middle negative pressure tube is used to adsorb the agar block, thereby reducing the acceleration of the agar block sliding in the perforated tube, making it easier to control the sliding distance of the agar block, so that the perforated tube can absorb more agar blocks.

[0021] In a specific possible implementation scheme, the control valve component includes an intermediate tube, a connecting hose and a control ball. One end of the intermediate hose is connected to the three-way pipe joint, and the other end is connected to the connecting hose. The connecting hose is connected to the intermediate negative pressure pipe. The intermediate tube forms a conical head at the end connected to the connecting hose. An extrusion spring is provided in the intermediate tube. The extrusion spring is connected to the control ball and can drive the control ball to abut against the conical head to form a closure of the connecting pipeline between the three-way pipe joint and the intermediate negative pressure pipe. An opening member is provided on the intermediate tube for driving the control ball to disengage from the conical head.

[0022] By adopting the above technical solution, when the negative pressure space and the middle negative pressure tube are isolated, the extrusion spring pushes the control ball to press against the conical head to seal the middle tube, thereby realizing the isolation of the negative pressure space and the middle negative pressure tube.

[0023] In a specific possible implementation scheme, the opening member includes an opening rod and a driving rod. The circumferential outward protrusion of the connecting hose forms a control groove. The driving rod is embedded in the control groove and is used to push the control ball out of the conical head. One end of the opening rod is fixedly connected to the control ball, and the other end is fixedly connected to the driving rod.

[0024] By adopting the above technical solution, when the negative pressure space is connected with the middle negative pressure tube, the driving rod is pushed toward the direction of the middle tube. The driving rod moves by utilizing the deformation of the connecting hose, and drives the control ball to detach from the conical head through the opening rod, thereby realizing the connection between the negative pressure space and the middle negative pressure tube.

[0025] In summary, the present application includes at least one of the following beneficial technical effects: 1. A negative pressure generating component generates negative pressure in the negative pressure space, and then a punching tube is inserted into the agar plate to perform a punching operation. The switch control component controls the negative pressure space to be connected with the punching tube, and the agar block generated after the punching is sucked into the punching tube under the action of negative pressure, thereby realizing the continuity of the punching and agar block removal steps, and there is no need to use a needle to remove the agar block, which effectively avoids damaging the hole edge, reduces the residue of agar blocks, reduces the impact on the experimental results, and improves the accuracy of the experimental results; 2. The balloon is connected to the negative pressure space through a three-way pipe joint. As the negative pressure in the negative pressure space decreases, the balloon will gradually swell from being deflated, playing the role of negative pressure monitoring, and the reflection of negative pressure is more intuitive; 3. Initially, the control valve is opened, the middle negative pressure tube is connected to the negative pressure space and the pressure is the same. When the agar block is adsorbed, the control valve is closed, and the negative pressure generated by the middle negative pressure tube is used to adsorb the agar block, reducing the acceleration of the agar block sliding in the perforated tube, making it easier to control the sliding distance of the agar block, so that the perforated tube can absorb more agar blocks. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the structure of an agar gel plate puncher in Example 1 of the present application.

[0027] Figure 2 is along Figure 1 Section view along line AA.

[0028] Figure 3 yes Figure 1 Enlarged view of part B in the middle.

[0029] Figure 4 yes Figure 2 Enlarged view of part C in the middle.

[0030] Figure 5 It is a schematic diagram of the structure of an agar gel plate puncher in Example 2 of the present application.

[0031] Figure 6 It is a schematic diagram used to show the structure of the fixing parts.

[0032] Figure 7 is along Figure 5 Sectional view along line DD.

[0033] Figure 8 It is a schematic diagram of the structure of an agar gel plate puncher in Example 3 of the present application.

[0034] Fig. 9 yes Figure 8 Enlarged view of middle part E.

[0035] Fig.10 It is a schematic diagram of the structure of an agar gel plate puncher according to Example 4 of the present application.

[0036] Fig.11 is along Fig.10 Cross-sectional view along line FF.

[0037] Fig.12 yes Fig.11 Enlarged view of the middle G section.

[0038] Explanation of reference numerals: 1. Negative pressure generating assembly; 11. Negative pressure space; 12. Syringe tube; 13. Piston; 14. Piston handle; 15. Exhaust pipe; 2. Perforated tube; 3. Switch control assembly; 31. Rubber tube; 32. Hard ball; 33. Glass branch tube; 34. Button; 4. Display assembly; 41. Airbag; 6. Three-way pipe joint; 7. Handle; 8. Fixing piece; 811. Card plate; 812. Card slot; 821. Spring clip; 822. Fixing part ;823, fixing hole;831, fixing rod;832, fixing spring;833, support plate;834, positioning hole;835, lever;836, positioning pin;91, intermediate negative pressure tube;92, control valve member;921, intermediate tube;922, connecting hose;923, control ball;924, conical head;925, support rod;926, extrusion spring;93, opening member;931, opening rod;932, driving rod;933, control groove. DETAILED DESCRIPTION

[0039] The following is combined with Figure 1-12 This application is described in further detail.

[0040] The embodiment of the present application discloses an agar gel plate puncher.

[0041] Example 1 Reference Figure 1 , Figure 2 An agar gel plate puncher includes a negative pressure generating component 1, a punching tube 2, a switch control component 3 and a display component 4. The negative pressure generating component 1 can generate a negative pressure space 11 inside, and the negative pressure space 11 is connected to the punching tube 2, and can suck agar blocks into the punching tube 2. The switch control component 3 is arranged between the negative pressure generating component 1 and the punching tube 2, and can control the on-off of the pipeline between the negative pressure space 11 and the punching tube 2. The display component 4 is connected to the negative pressure space 11 and can display the pressure state of the negative pressure space 11.

[0042] When punching holes in the agar plate, firstly, negative pressure is generated in the negative pressure space 11 through the negative pressure generating component 1, and then the punching tube 2 is inserted into the agar plate for punching operation, and then the switch control component 3 is used to control the negative pressure space 11 to be connected with the punching tube 2, and the agar block generated after the punching is sucked into the punching tube 2 under the action of negative pressure, and when the agar block moves a distance greater than the length of the agar block in the punching tube 2, the switch control component 3 controls the negative pressure space 11 to be disconnected from the punching tube 2, and then the punching tube 2 performs the next punching operation, and then the switch control component 3 controls the connection and disconnection of the negative pressure space 11 and the punching tube 2 to suck the agar block into the punching tube 2, so as to realize the continuity of the punching and agar block removal steps, and there is no need to use a needle to remove the agar block, which effectively avoids damaging the hole edge, reduces the residue of agar block, reduces the influence on the experimental results, and improves the accuracy of the experimental results. As the agar block is adsorbed, the display component 4 intuitively reflects the change of the negative pressure in the negative pressure space 11, which facilitates people to control the adsorption of the agar block.

[0043] Reference Figure 1 , Figure 2 The negative pressure generating assembly 1 in this embodiment includes a syringe tube 12, a piston 13 and a piston handle 14. The syringe tube 12 and the piston handle 14 are made of plastic material, and the piston 13 is made of rubber material. The piston 13 is slidably sealed and arranged in the syringe tube 12. The end of the syringe tube 12 bulges outward to form an air outlet pipe 15, which drives the piston 13 to slide, and a negative pressure space 11 is formed between the piston 13 and the air outlet pipe 15. The piston handle 14 in this embodiment is a four-sided piston handle. The piston handle 14 is fixedly installed on the side of the piston 13 away from the negative pressure space 11. The four edges of the piston handle 14 are arranged perpendicular to each other. A fixing member 8 is provided on the syringe tube 12. When the piston 13 is pulled to generate the negative pressure space 11, the piston 13 is fixed by the fixing member 8. The piston 13 is pulled to move by the piston handle 14 to generate the negative pressure space 11 in the syringe tube 12. The structure is simple, easy to operate, and easy to carry. The position of the piston 13 is fixed by the fixing member 8 to improve the stability of the negative pressure space 11.

[0044] Reference Figure 1 , Figure 3, in this embodiment, the fixing member 8 includes a clamping plate 811. The clamping plate 811 is in a U shape and is horizontally and fixedly installed at the open end of the syringe tube 12. The piston handle 14 passes through the space formed in the middle of the clamping plate 811. Two edges of the piston handle 14 are in contact with one side of the clamping plate 811, and the other two edges are in contact with the other side of the clamping plate 811, so that the clamping plate 811 restricts the rotation of the piston handle 14. The piston handle 14 is provided with a slot 812 for the clamping plate 811 to insert on each edge. When the piston handle 14 drives the slot 812 to rotate to a position opposite to the clamping plate 811, the piston handle 14 is rotated to make the clamping plate 811 insert into the slot 812, thereby realizing the fixation of the piston handle 14 and ensuring the stability of the negative pressure space 11.

[0045] Refer to Figure 1 , Figure 2 , a three-way pipe joint 6 is connected to the end of the air outlet pipe 15. The display component 4 in this embodiment is an airbag 41. The airbag 41 is connected to one of the joints of the three-way pipe joint 6. When pulling the piston 13 to generate the negative pressure space 11, the airbag 41 deflates. As the negative pressure space 11 continuously sucks in the agar block, the pressure in the negative pressure space 11 is continuously increasing. At this time, the airbag 41 gradually bulges. By the state of passing through the airbag 41, the monitoring of the negative pressure is realized, and the negative pressure can be more intuitively reflected.

[0046] Refer to Figure 2 , Figure 4 , the switch control component 3 in this embodiment includes a rubber tube 31 and a hard ball 32. The hard ball 32 in this embodiment is a glass ball. One end of the rubber tube 31 is connected to one of the joints of the three-way pipe joint 6 through a glass tube, and the other end is connected to the punching tube 2. The hard ball 32 is plugged into the rubber tube 31. The diameter of the hard ball 32 is larger than the inner diameter of the rubber tube 31 in its natural state, ensuring that when the hard ball 32 is stuffed into the rubber tube 31, the hard ball 32 is tightly pressed against the inner wall of the rubber tube 31 to realize the blocking of the rubber tube 31. A glass branch tube 33 is connected between the glass tube and the punching tube 2. The rubber tube 31 and the hard ball 32 are both located inside the glass branch tube 33. A key 34 is provided at a position opposite to the hard ball 32 on the glass branch tube 33. The key 34 is slidably arranged along the radial direction of the glass branch tube 33.

[0047] When connecting the negative pressure space 11 with the punching tube 2, the operator presses the key 34 to press the rubber tube 31 against the hard ball 32, so that the contact part between the rubber tube 31 and the hard ball 32 deforms to generate an opening, thereby realizing the connection between the negative pressure space 11 and the punching tube 2. When disconnecting the negative pressure space 11 from the punching tube 2, the key 34 is released, and the rubber tube 31 returns to a state of being tightly attached to the hard ball 32, thereby realizing the disconnection between the negative pressure space 11 and the punching tube 2, and improving the sensitivity of the on-off control between the negative pressure space 11 and the punching tube 2.

[0048] Refer to Figure 1 A handle 7 is provided on the perforated tube 2 to facilitate holding the perforated tube 2.

[0049] In immunological testing, the commonly used sample volume in the gel hole is 5ul or 10ul, which can correspond to 2mm or 3mm perforating tube 2 respectively. This example tests the punching success rate of these two punchers. According to the implementation plan, 5ml and 10ml syringe punchers (diameter 2mm and 3mm perforating tube 2) are used to punch holes on the paved agar gel plate (thickness 2mm). Each puncher punches 3 batches, 100 holes per batch, and if agar blocks remain in the hole, it means that the punching fails. Calculate the punching success rate (%): Punching success rate (%) = number of residual agar holes / total number of punched holes * 100% Comparative Example 1: No airbag 41 negative pressure monitoring; It can be seen that the drilling success rate of the airbag 41 with negative pressure monitoring is greater than the success rate without negative pressure monitoring.

[0050] The implementation principle of Example 1 is as follows: when punching, first pull open the syringe piston handle 14 so that the slots 812 on the four edges are opposite to the card plate 811, then rotate the piston handle 14 at a 45-degree angle to clamp it. By fixing the piston handle 14, negative pressure can be formed in the syringe, and the balloon is completely deflated due to the negative pressure, indicating the existence of negative pressure. Then the operator holds the handle 7 with one hand and inserts the punching tube 2 vertically to the bottom of the gel plate according to the pre-designed template. The other hand presses the button 34 to open the air circuit, so that the negative pressure space 11 and the punching tube 2 air circuit are connected and form an equal negative pressure, and then releases the button 34, and the air circuit is closed. At this time, the negative pressure formed in the handle 7 can only clamp the agar block but cannot be sucked into the tube. Lift the punching tube 2. At this time, the inside of the punching tube 2 is in a closed state, and the negative pressure is equal to the negative pressure of the negative pressure space 11. Due to the internal and external pressure difference, the agar block is sucked into the puncher cavity. During the continuous punching process, the negative pressure is gradually released and the balloon gradually bulges, indicating that the negative pressure has been basically released and the glue block in the hole can no longer be sucked in. The syringe needs to be pulled out again to form negative pressure. Before pulling out again to form negative pressure, press the button 34 first, pull out repeatedly, and discharge the sucked agar block. If the discharge is not smooth, a small amount of water can be sucked into the handle 7, and then the water and glue are discharged together.

[0051] The continuity of the steps of punching and taking out the agar block is achieved, and there is no need to use a needle to take out the agar block, which effectively avoids damaging the edge of the hole, reduces the residue of the agar block, reduces the impact on the experimental results, and improves the accuracy of the experimental results.

[0052] Example 2 Reference Figure 5 , Figure 6 and Figure 7 The difference between this embodiment and embodiment 1 is that the fixing member 8 in this embodiment includes a spring clip 821 and a fixing portion 822, the spring clip 821 is arranged on the piston handle 14, and the fixing portion 822 is arranged on the spring clip 821, and the spring clip 821 can push the fixing portion 822 to expand outward so that the fixing portion 822 abuts against the inner wall of the syringe tube 12, and a fixing hole 823 is provided on the inner wall of the syringe tube 12, and the fixing hole 823 is located on the sliding track of the fixing portion 822.

[0053] The implementation principle of Example 2 is as follows: when the fixing portion 822 slides to be opposite to the fixing hole 823 , the spring clip 821 pushes the fixing portion 822 to be inserted into the fixing hole 823 , thereby completing the fixed connection between the piston 13 and the syringe tube 12 .

[0054] Example 3 Reference Figure 8 , Fig. 9 The difference between this embodiment and embodiment 1 is that the fixing member 8 in this embodiment includes a fixing rod 831 and a fixing spring 832, a support plate 833 is fixedly provided at the open end of the syringe tube 12, the fixing rod 831 is slidably set on the support plate 833 through a guide rail, and is set toward the piston handle 14, a baffle is provided at the edge of the support plate 833, a positioning hole 834 for inserting the fixing rod 831 is opened on the piston handle 14, a horizontally placed shifting rod 835 is fixedly provided on the fixing rod 831, a fixing spring 832 is sleeved on the fixing rod 831, one end of the fixing spring 832 is fixedly connected to the baffle, and the other end is fixedly connected to the shifting rod 835, a positioning pin 836 for blocking the movement of the shifting rod 835 is provided on the support plate 833, and initially, the fixing spring 832 pushes the fixing rod 831 to slide on the piston handle 14.

[0055] The implementation principle of Example 3: when the piston handle 14 is pulled to move and generate the negative pressure space 11, the piston handle 14 drives the positioning hole 834 to slide. When the positioning hole 834 slides to a position relative to the fixing rod 831, the fixing spring 832 pushes the fixing rod 831 to insert into the positioning hole 834, thereby fixing the piston handle 14.

[0056] Example 4 Reference Fig.10 , Fig.11 The difference between this embodiment and embodiment 1 is that the three-way pipe joint 6 is connected to the rubber tube 31 and is provided with an intermediate negative pressure tube 91. The diameter of the intermediate negative pressure tube 91 is larger than the diameter of the perforated tube 2 but smaller than the diameter of the syringe tube 12. The intermediate negative pressure tube 91 is connected to the three-way pipe joint 6 by a control valve component 92. The control valve component 92 is used to control the connection and disconnection of the intermediate negative pressure tube 91 and the perforated tube 2.

[0057] When the negative pressure space 11 sucks the agar block into the perforated tube 2, the change in the volume of the agar block in the perforated tube 2 is not obvious relative to the negative pressure space 11, so that the acceleration of the agar block sliding in the perforated tube 2 due to the negative pressure adsorption of the negative pressure space 11 is large, and it is not easy to control the sliding distance of the agar block. For this reason, through the setting of the middle negative pressure tube 91, the change in the volume of the agar block in the perforated tube 2 is made more obvious, the acceleration of the agar block sliding in the perforated tube 2 is reduced, the convenience of controlling the sliding distance of the agar block is improved, and the distance between the two agar blocks is reduced, so that more agar blocks can be stored in the perforated tube 2.

[0058] Reference Fig.11 , Fig.12 The control valve member 92 in this embodiment includes an intermediate tube 921, a connecting hose 922 and a control ball 923. One end of the intermediate hose is connected to the three-way pipe joint 6, and the other end is connected to the connecting hose 922. The connecting hose 922 is connected to the intermediate negative pressure pipe 91. The intermediate tube 921 forms a conical head 924 at one end connected to the connecting hose 922. The intermediate tube 921 is provided with a support rod 925 at the upstream of the conical head 924. An extrusion spring 926 is provided in the intermediate tube 921. One end of the extrusion spring 926 is fixedly connected to the support rod 925, and the other end is fixedly connected to the control ball 923. The control ball 923 in this embodiment is a rubber ball. The extrusion spring 926 abuts the control ball 923 against the conical head 924, forming a closure of the pipeline connecting the three-way pipe joint 6 and the intermediate negative pressure pipe 91.

[0059] Reference Fig.11 , Fig.12 An opening piece 93 for driving the control ball 923 to disengage from the conical head 924 is provided on the middle tube 921. The opening piece 93 in this embodiment includes an opening rod 931 and a driving rod 932. The circumferential outward protrusion of the connecting hose 922 forms a control groove 933. The driving rod 932 is embedded in the control groove 933 and is used to push the control ball 923 to disengage from the conical head 924. One end of the opening rod 931 is fixedly connected to the control ball 923, and the other end is fixedly connected to the driving rod 932.

[0060] When the negative pressure space 11 is isolated from the middle negative pressure tube 91, the extrusion spring 926 pushes the control ball 923 against the conical head 924 to block the middle tube 921, thereby realizing the isolation of the negative pressure space 11 from the middle negative pressure tube 91. When the negative pressure space 11 is connected to the middle negative pressure tube 91, the driving rod 932 is pushed toward the middle tube 921, and the driving rod 932 moves by the deformation of the connecting hose 922, and drives the control ball 923 to disengage from the conical head 924 through the opening rod 931, thereby realizing the connection between the negative pressure space 11 and the middle negative pressure tube 91. When the agar block in the perforated tube 2 is discharged, the operator needs to maintain the upward push action of the driving rod 932 so that the middle tube 921 is always in an open state.

[0061] The implementation principle of Example 4 is as follows: by setting the middle negative pressure tube 91, the change in the volume of the agar block in the perforated tube 2 is made more obvious, the acceleration of the agar block sliding in the perforated tube 2 is reduced, the convenience of controlling the sliding distance of the agar block is improved, and the distance between the two agar blocks is reduced, so that more agar blocks can be stored in the perforated tube 2.

[0062] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. An agar gel plate puncher, characterized in that: The invention comprises a negative pressure generating component (1), a perforated tube (2), a switch control component (3) and a display component (4); the negative pressure generating component (1) can generate a negative pressure space (11) inside; the negative pressure space (11) is connected to the perforated tube (2) so that agar blocks can be sucked into the perforated tube (2); the switch control component (3) is arranged between the negative pressure generating component (1) and the perforated tube (2) so as to control the on / off of the pipeline between the negative pressure space (11) and the perforated tube (2); the display component (4) is connected to the negative pressure space (11) and can display the pressure state of the negative pressure space (11).

2. The agar gel plate puncher according to claim 1, characterized in that: The negative pressure generating assembly (1) comprises a syringe tube (12), a piston (13) and a piston handle (14); the piston (13) is slidably arranged in the syringe tube (12) and forms the negative pressure space (11) in the syringe tube (12); an air outlet pipe (15) connected to the negative pressure space (11) is formed at the end of the syringe tube (12); the piston handle (14) is fixedly arranged on a side of the piston (13) away from the negative pressure space (11); and a fixing member (8) is provided on the syringe tube (12) for limiting the movement of the piston (13) under the action of the negative pressure space (11).

3. The agar gel plate puncher according to claim 2, characterized in that: The fixing member (8) comprises a clamping plate (811) arranged on the syringe tube (12); a clamping groove (812) cooperating with the clamping plate (811) is provided on the piston handle (14); the clamping plate (811) can be rotated so that the clamping plate (811) is inserted into the clamping groove (812) to fix the clamping plate (811) and the piston handle (14).

4. The agar gel plate puncher according to claim 2, characterized in that: The fixing member (8) comprises a spring clip (821) and a fixing portion (822), wherein the fixing portion (822) is arranged on the spring clip (821), the spring clip (821) and the piston (13) are fixedly arranged, and the syringe tube (12) is provided with a fixing hole (823) for the fixing portion (822) to be inserted, and the spring clip (821) can push the fixing portion (822) to be inserted into the fixing hole (823) so as to fix the piston (13) and the syringe tube (12).

5. The agar gel plate puncher according to claim 2, characterized in that: The fixing member (8) comprises a fixing rod (831) and a fixing spring (832); the fixing rod (831) is slidably arranged on the syringe tube (12) toward the piston handle (14); a positioning hole (834) for inserting the fixing rod (831) is provided on the piston handle (14); the fixing spring (832) is connected to the syringe tube (12) and the fixing rod (831) so as to be able to drive the fixing rod (831) to be inserted into the positioning hole (834).

6. The agar gel plate puncher according to claim 2, characterized in that: The switch control assembly (3) comprises a rubber tube (31) and a hard ball (32). The rubber tube (31) is connected between the air outlet tube (15) and the perforated tube (2). The hard ball (32) is arranged in the rubber tube (31) and is used to control the opening and closing of the rubber tube (31). The hard ball (32) can squeeze the rubber tube (31) to bulge outwards and press the rubber tube (31) onto the hard ball (32) so that the rubber tube (31) is opened.

7. The agar gel plate puncher according to claim 6, characterized in that: The display assembly (4) comprises a balloon, and the balloon is connected to the air outlet pipe (15) and the rubber tube (31) via a three-way pipe joint (6).

8. The agar gel plate puncher according to claim 7, characterized in that: The three-way pipe joint (6) is connected to the rubber tube (31) and is provided with an intermediate negative pressure pipe (91). The intermediate negative pressure pipe (91) and the three-way pipe joint (6) are connected via a control valve component (92). The control valve component (92) is used to control the connection and disconnection of the intermediate negative pressure pipe (91) and the perforated pipe (2).

9. The agar gel plate puncher according to claim 8, characterized in that: The control valve component (92) comprises an intermediate tube (921), a connecting hose (922) and a control ball (923). One end of the intermediate hose is connected to the three-way pipe joint (6), and the other end is connected to the connecting hose (922). The connecting hose (922) is connected to the intermediate negative pressure pipe (91). The intermediate tube (921) forms a conical head (924) at the end connected to the connecting hose (922). An extrusion spring (926) is provided in the intermediate tube (921). The extrusion spring (926) is connected to the control ball (923) and can drive the control ball (923) to abut against the conical head (924) to close the connecting pipeline between the three-way pipe joint (6) and the intermediate negative pressure pipe (91). An opening member (93) is provided on the intermediate tube (921) for driving the control ball (923) to separate from the conical head (924).

10. The agar gel plate puncher according to claim 9, characterized in that: The opening member (93) includes an opening rod (931) and a driving rod (932). The circumferential outward protrusion of the connecting hose (922) forms a control groove (933). The driving rod (932) is embedded in the control groove (933) and is used to push the control ball (923) out of the conical head (924). One end of the opening rod (931) is fixedly connected to the control ball (923), and the other end is fixedly connected to the driving rod (932).

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