Preparation method and application of water-soluble zinc oxide

Abstract

The invention belongs to the technical field of feed, discloses a preparation method and application of water-soluble zinc oxide, and particularly discloses a stomach-passing coating material which comprises acrylate, water-soluble sugar and water-soluble salt. The invention provides a stomach-passing coating material which can ensure that zinc oxide is not damaged by gastric acid and can be directly absorbed and utilized in intestinal tracts. The water-soluble zinc oxide is further prepared by using the stomach-passing coating material, and the water-soluble zinc oxide not only can be added into a feed, but also can be directly added into drinking water of livestock and poultry, so that a higher dose of zinc oxide can reach intestinal tracts to play a role, the diarrhea rate of piglets in a weaning stage is reduced, and the breeding cost is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of feed, and particularly relates to a preparation method of water-soluble zinc oxide and application thereof. Background Art

[0002] Zinc is an essential trace element for animals. It is directly involved in the metabolism of proteins, fats and carbohydrates in animals and plays an important role in improving the body's immune and disease resistance, antioxidant capacity and production performance. In addition, zinc is also directly involved in the synthesis of nucleic acids and proteins, redox, cellular immunity and humoral immunity.

[0003] The absorption of ordinary zinc is not ideal. Some of the zinc that is not absorbed by the animal body will be excreted with feces, causing pollution to the soil and rivers. Zinc deficiency can easily lead to metabolic disorders, decreased immunity, and affect animal growth. Studies have shown that nano zinc oxide is the best zinc source. It has a very high absorption and utilization rate in the animal body and is widely distributed inside the body. It can directly avoid adverse reactions with other drugs through penetration and consolidate the absorption effect.

[0004] In 1989, Poulsen first reported that pharmacological doses of zinc oxide (2500-4000 mg / kg) can be used to prevent and treat diarrhea in weaned piglets and promote their growth and development. High doses of zinc are beneficial to the improvement of the intestines of weaned piglets and reduce the diarrhea rate. Fang Rui et al. (2017) added 2500 mg / kg of high-dose zinc oxide to the diet of 24-day-old weaned piglets and found that the average daily weight gain and average daily feed intake of piglets were significantly higher than those of the low-zinc group after 14 days, the feed-to-meat ratio was reduced, and the diarrhea rate was significantly reduced. High doses of zinc oxide also increased the apparent digestibility of dry matter, crude protein and crude fat in the diet of weaned piglets. Early weaning of piglets will cause intestinal villi atrophy, reduce villus height, and increase crypt depth, resulting in obstruction of nutrient digestion and absorption. Undigested substances enter the hind intestine and are used by harmful microorganisms, causing excessive reproduction of harmful microorganisms, which can easily cause diarrhea. When piglets were fed a diet containing high doses of zinc oxide, the height of the villi in the duodenum and ileum increased, and the ratio of the villi height to the crypt depth in the jejunum and ileum increased significantly. This proves that high doses of zinc oxide reduce the diarrhea rate in early weaned piglets by improving the intestinal structure of piglets. The effect of high doses of zinc oxide on the permeability of piglet mucosa. The study suggests that zinc oxide prevents diarrhea by reducing bacterial adhesion and invasion of intestinal cells and preventing the increase in tight junction permeability.

[0005] Zinc oxide is an amphiphilic molecule that is almost insoluble in water. When the pH in the stomach is 3.5-4.5, most of the insoluble zinc oxide is converted into zinc ions, and a small amount of zinc oxide can work in the intestines. Zinc oxide can effectively inhibit harmful microorganisms in the intestines of animals and promote rapid growth of animals. Most of the zinc oxide on the market is water-insoluble zinc oxide. For example, Chinese invention patent CN109511786A points out a method for preparing a feed additive containing zinc oxide, which is water-insoluble and has less zinc oxide that actually reaches the intestines of animals to play a role. Although Chinese invention patent CN102659171A points out a method for preparing zinc oxide nanoparticles, the zinc oxide nanoparticles are not feed additives and have no related breeding applications.

[0006] Zinc oxide is an amphoteric molecule that is almost insoluble in water. When the pH in the stomach is 3.5-4.5, most of the insoluble zinc oxide is converted into zinc ions, and a small amount of zinc oxide can work in the intestine. Therefore, the absorption of ordinary zinc is not ideal. Some zinc that is not absorbed by the animal body will be excreted with feces, causing pollution to the soil and rivers. Zinc deficiency can easily lead to metabolic disorders, decreased immunity, and affect animal growth. Studies have shown that nano zinc oxide is the best zinc source, with a very high absorption and utilization rate in the animal body, and is widely distributed inside the body. It can directly avoid adverse reactions with other drugs through penetration and consolidate the absorption effect. Summary of the invention

[0007] In view of the technical problems existing in the prior art, the present invention aims to provide a gastric coating material and nano zinc oxide that can be dispersed in water and a preparation method thereof. The gastric coating material can ensure that zinc oxide will not be destroyed by gastric acid and can be directly absorbed and utilized in the intestine.

[0008] The first aspect of the present invention aims to provide a gastric coating material.

[0009] The second aspect of the present invention aims to provide a water-soluble zinc oxide.

[0010] The third aspect of the present invention aims to provide a method for preparing the water-soluble zinc oxide of the second aspect of the present invention.

[0011] The purpose of the fourth aspect of the present invention is to provide use of the water-soluble zinc oxide of the second aspect of the present invention in preparing a product.

[0012] The fifth aspect of the present invention aims to provide a feed.

[0013] In order to achieve the above object, the technical solution adopted by the present invention is:

[0014] The first aspect of the present invention provides a gastric coating material comprising acrylate, water-soluble sugar and water-soluble salt.

[0015] In some embodiments of the present invention, the gastric coating material includes, by weight, 1 to 8 parts of acrylate, 1 to 5 parts of water-soluble sugar and 1 to 6 parts of water-soluble salt.

[0016] In some embodiments of the present invention, the gastric coating material includes, by weight, 4 to 8 parts of acrylate, 1 to 4 parts of water-soluble sugar and 1 to 4 parts of water-soluble salt.

[0017] In some embodiments of the present invention, the gastric coating material includes 4 to 6 parts of acrylate, 1 to 2 parts of water-soluble sugar and 2 to 4 parts of water-soluble salt, by weight.

[0018] In some embodiments of the present invention, the acrylate salt includes at least one of sodium acrylate salt, potassium acrylate salt, ammonium acrylate salt, magnesium acrylate salt, and calcium acrylate salt having a molecular weight of 3,000 to 1,000,000.

[0019] In some embodiments of the present invention, the acrylate includes at least one of ammonium polyacrylate, potassium polyacrylate, and sodium polyacrylate.

[0020] In some embodiments of the present invention, the water-soluble salt includes at least one of sodium citrate, potassium citrate, sodium succinate, sodium chloride, potassium chloride, calcium chloride, potassium phosphate, magnesium phosphate, sodium phosphate, sodium adipate, sodium fumarate, sodium benzoate, and potassium sorbate.

[0021] In some embodiments of the present invention, the water-soluble salt includes sodium citrate and / or potassium citrate.

[0022] In some embodiments of the present invention, the water-soluble sugar includes at least one of monosaccharides, disaccharides and polysaccharides.

[0023] In some embodiments of the present invention, the monosaccharide includes at least one of glucose, mannose, ribose, xylose, arabinose, rhamnose, mannose, galactose, and fructose.

[0024] In some embodiments of the present invention, the disaccharide includes at least one of sucrose, gentiobiose, maltose, cellobiose, lactose and laminaribiose.

[0025] In some embodiments of the present invention, the polysaccharide includes at least one of starch, glycogen, and raffinose, and the starch is soluble modified starch.

[0026] In some embodiments of the present invention, the starch comprises hydroxypropyl starch.

[0027] The second aspect of the present invention provides a water-soluble zinc oxide, which is made from the following raw materials: the gastric coating material of the first aspect of the present invention and zinc oxide.

[0028] In some embodiments of the present invention, the water-soluble zinc oxide is made of the following raw materials, calculated by weight: 3 to 20 parts of gastric coating material and 15 to 30 parts of zinc oxide.

[0029] In some embodiments of the present invention, the water-soluble zinc oxide is made of the following raw materials, calculated by weight: 5 to 15 parts of gastric coating material and 20 to 25 parts of zinc oxide.

[0030] In some embodiments of the present invention, the particle size of the water-soluble zinc oxide is 180 to 210 nm.

[0031] In some embodiments of the present invention, the zinc oxide accounts for 50 wt% to 75 wt% of the water-soluble zinc oxide, preferably 65 wt%.

[0032] The water-soluble sugar in water-soluble zinc oxide has a large number of network structures, which can tightly wrap the tiny zinc oxide particles through the intermolecular force. This can reduce the agglomeration of zinc oxide particles in solution and after drying, and also reduce the contact between zinc oxide and gastric acid in the stomach, reducing the loss of zinc oxide. In the intestine, zinc oxide will be released under the action of amylase and the like. Due to its nanometer particle size, it can be better absorbed and utilized by tissues, solving the problem of low utilization of zinc oxide and waste of resources. Adding acrylate can make the network structure of starch and acrylic acid more stable, so that the zinc oxide grinding liquid is stable for a long time. Due to the presence of water-soluble salts and water-soluble sugars, the dried zinc oxide powder can dissolve faster.

[0033] The third aspect of the present invention provides a method for preparing the water-soluble zinc oxide of the second aspect of the present invention, comprising the following steps: dissolving the gastric coating material in water, mixing evenly, and mixing with zinc oxide to obtain a mixture; and homogenizing the mixture to obtain water-soluble zinc oxide.

[0034] In some embodiments of the present invention, the homogenization conditions are 20-50° C., 20-100 MPa, and the homogenization times are 1 to 5 times.

[0035] In some embodiments of the present invention, the homogenization conditions are 40-50° C., 40-50 MPa, and the homogenization times are 3-4 times.

[0036] In some embodiments of the present invention, the homogenization is performed by grinding with a homogenizer, such as a high-pressure homogenizer or a basket homogenizer.

[0037] In some embodiments of the present invention, the zinc oxide is subjected to ultrafine grinding and passed through a 1200-1300 mesh sieve before mixing.

[0038] In some embodiments of the present invention, the preparation method further comprises a drying step, wherein the drying is performed by vacuum drying, air drying, freeze drying, etc.; preferably, a centrifugal drying tower is used for the drying.

[0039] In some embodiments of the present invention, the air inlet temperature of the centrifugal drying tower is controlled at 150-250°C, and the air outlet temperature is controlled at 60-100°C.

[0040] The fourth aspect of the present invention provides the use of the water-soluble zinc oxide according to the second aspect of the present invention in the preparation of a product, wherein the product includes at least one of a reagent, a kit, a medicine, a feed, a cosmetic, a bactericide, and an aqueous conductive material.

[0041] In some embodiments of the present invention, the product has at least the following functions: improving animal immunity, promoting animal growth, reducing animal diarrhea rate, treating tumors, drug delivery, fluorescence imaging, cell labeling, conductivity, antibacterial, photocatalyst, catalyst carrier, sewage pollutant adsorption, etc.

[0042] A fifth aspect of the present invention provides a feed comprising the water-soluble zinc oxide according to the second aspect of the present invention.

[0043] In some embodiments of the present invention, the feed further comprises a basal diet.

[0044] In some embodiments of the present invention, the basal diet comprises.

[0045] In some embodiments of the present invention, the basic diet includes soybean oil, corn, soybean meal, soybeans, whey powder, plasma protein powder, wheat hydrolyzed protein, fish meal, sucrose, soybean oil, glucose, calcium hydrogen phosphate, yeast extract, stone powder, sodium chloride, choline chloride, zinc oxide, acidifier, mildew inhibitor, enzyme preparation, phytase, sweetener, amino acid and multivitamin and multi-mineral premix.

[0046] In some embodiments of the present invention, the amino acids include lysine, methionine, threonine, tryptophan and valine.

[0047] In some embodiments of the present invention, the multivitamin and multimineral premix includes vitamin A, vitamin B2, pantothenic acid, vitamin B 12 , Vitamin D3, Vitamin E, Vitamin K, Biotin, Niacin, Copper, Iron, Manganese, Zinc, Iodine and Selenium.

[0048] In some embodiments of the present invention, the feed contains 0.1 wt% to 0.5 wt% of water-soluble zinc oxide.

[0049] The beneficial effects of the present invention are:

[0050] The invention provides a gastric coating material, which can ensure that zinc oxide will not be destroyed by gastric acid and can be directly absorbed and utilized in the intestinal tract.

[0051] The present invention provides a water-soluble zinc oxide, which can not only reduce the production cost of zinc oxide products, but also enable a higher dose of zinc oxide to reach the intestine to exert its effects, thereby reducing the diarrhea rate of piglets during the weaning stage and reducing breeding costs. The water-soluble zinc oxide can be added not only to feed, but also directly to the drinking water of livestock and poultry, and smoothly enter the intestine of the animal to exert its effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 This is an electron microscope image of the water-soluble zinc oxide prepared in Example 1.

[0053] Figure 2 This is an electron microscope image of the water-soluble zinc oxide prepared in Example 1.

[0054] Figure 3 This is an electron microscope image of the water-soluble zinc oxide prepared in Example 2.

[0055] Figure 4 This is an electron micrograph of the particle coating layer of the water-soluble zinc oxide prepared in Example 1.

[0056] Figure 5 This is an electron micrograph of the particle coating layer of the water-soluble zinc oxide prepared in Example 2. DETAILED DESCRIPTION

[0057] The present invention is further described in detail below through specific examples.

[0058] It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0059] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0060] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0061] Example 1

[0062] A method for preparing water-soluble zinc oxide comprises the following steps:

[0063] Take 10,000 grams of water, add 200 grams of sodium polyacrylate with a molecular weight of 10,000 to 30,000 and 300 grams of potassium polyacrylate with a molecular weight of 5,000 to 10,000, stir and dissolve, then add 300 grams of potassium citrate and 200 grams of glucose, stir and dissolve, then add 2,300 grams of zinc oxide that has been ultrafinely crushed and passed through a 1,250-mesh sieve, stir and disperse, then add the mixed solution into a homogenizer with 45 MPa, a feed liquid temperature of 45° C., and a homogenization number of 4 times. After homogenization, dry it in a centrifugal drying tower, the air inlet temperature of the centrifugal drying tower is controlled at 200° C., and the air outlet temperature is 100° C. After drying, collect the material, pass it through a 20-100 mesh sieve, and obtain water-soluble zinc oxide.

[0064] The electron microscope image of the prepared water-soluble zinc oxide is as follows: Figure 1 , Figure 2 and Figure 4 shown.

[0065] Example 2

[0066] A method for preparing water-soluble zinc oxide comprises the following steps:

[0067] Take 10,000 grams of water, add 150 grams of ammonium polyacrylate with a molecular weight of 3,000 to 20,000, 400 grams of potassium polyacrylate with a molecular weight of 5,000 to 10,000, stir and dissolve, then add 100 grams of maltose syrup, 50 grams of hydroxypropyl starch, 50 grams of lactose, and 300 grams of sodium citrate, stir and dissolve, then add 2,000 grams of zinc oxide that has been ultrafinely crushed and passed through a 1,250-mesh sieve, stir and disperse, then add the mixed solution into a homogenizer with 55 MPa, a feed liquid temperature of 50° C., and a homogenization number of 4 times. After homogenization, dry it in a centrifugal drying tower, the air inlet temperature of the centrifugal drying tower is controlled at 190° C., and the air outlet temperature is 95° C. After drying, collect the material, pass it through a 20-100 mesh sieve, and obtain water-soluble zinc oxide.

[0068] The electron microscope image of the prepared water-soluble zinc oxide is as follows: Figure 3 and Figure 5 shown.

[0069] Comparative Example 1

[0070] A method for preparing zinc oxide, which differs from Example 1 only in that no water-soluble salt is added (ie, no potassium citrate is added).

[0071] Specifically as follows: take 10,000 grams of water, add 200 grams of sodium polyacrylate with a molecular weight of 10,000 to 30,000 and 300 grams of potassium polyacrylate with a molecular weight of 5,000 to 10,000, stir and dissolve, then add 200 grams of glucose and stir and dissolve, then add 2,300 grams of zinc oxide that has been ultrafinely crushed and passed through a 1,250-mesh sieve and stir and disperse, then add the mixed solution into a homogenizer with a pressure of 45 MPa, a feed liquid temperature of 45°C, and a homogenization number of 4 times. After homogenization, dry it in a centrifugal drying tower, the air inlet temperature of the centrifugal drying tower is controlled at 200°C, and the air outlet temperature is 100°C. After drying, collect the material, pass it through a 20-100 mesh sieve to obtain zinc oxide.

[0072] Comparative Example 2

[0073] A method for preparing zinc oxide, which is different from Example 1 only in that no water-soluble sugar is added (ie, no glucose is added).

[0074] Specifically as follows: take 10,000 grams of water, add 200 grams of sodium polyacrylate with a molecular weight of 10,000 to 30,000 and 300 grams of potassium polyacrylate with a molecular weight of 5,000 to 10,000, stir and dissolve, then stir and dissolve 300 grams of potassium citrate, then add 2,300 grams of zinc oxide that has been ultrafinely crushed and passed through a 1,250-mesh sieve and stir and disperse, then add the mixed solution into a homogenizer with a pressure of 45 MPa, a feed liquid temperature of 45°C, and a homogenization number of 4 times. After homogenization, dry it in a centrifugal drying tower, the air inlet temperature of the centrifugal drying tower is controlled at 200°C, and the air outlet temperature is 100°C. After drying, collect the material, pass it through a 20-100 mesh sieve to obtain zinc oxide.

[0075] Comparative Example 3

[0076] A method for preparing zinc oxide, which differs from Example 2 only in that no water-soluble salt is added (ie, no sodium citrate is added).

[0077] Take 10,000 grams of water, add 150 grams of ammonium polyacrylate with a molecular weight of 3,000 to 20,000, 400 grams of potassium polyacrylate with a molecular weight of 5,000 to 10,000, stir and dissolve, then add 100 grams of maltose syrup, 50 grams of hydroxypropyl starch, and 50 grams of lactose, stir and dissolve, then add 2,000 grams of zinc oxide that has been ultrafinely crushed and passed through a 1,250-mesh sieve, stir and disperse, then add the mixed solution into a homogenizer with 55 MPa, a feed liquid temperature of 50° C., and a homogenization number of 4 times. After homogenization, dry it in a centrifugal drying tower, the air inlet temperature of the centrifugal drying tower is controlled at 190° C., and the air outlet temperature is 95° C. After drying, collect the material, pass it through a 20-100 mesh sieve, and obtain zinc oxide.

[0078] Comparative Example 4

[0079] A method for preparing zinc oxide, which is different from Example 2 only in that no water-soluble sugar is added (ie, no maltose syrup, hydroxypropyl starch and lactose are added).

[0080] The details are as follows: take 10,000 grams of water, add 150 grams of ammonium polyacrylate with a molecular weight of 3,000 to 20,000, 400 grams of potassium polyacrylate with a molecular weight of 5,000 to 10,000, stir and dissolve, then add 300 grams of sodium citrate and stir and dissolve, then add 2,000 grams of zinc oxide that has been ultrafinely crushed and passed through a 1,250-mesh sieve and stir and disperse, then add the mixed solution into a homogenizer with 55 MPa, a feed liquid temperature of 50°C, and a homogenization number of 4 times. After homogenization, dry it in a centrifugal drying tower, the air inlet temperature of the centrifugal drying tower is controlled at 190°C, and the air outlet temperature is at 95°C. After drying, collect the material, pass it through a 20-100 mesh sieve, and obtain zinc oxide.

[0081] Comparative Example 5

[0082] A method for preparing zinc oxide, which is different from Example 1 only in that no water-soluble sugar and water-soluble salt are added (ie, no glucose and potassium citrate are added).

[0083] The details are as follows: take 10,000 grams of water, add 200 grams of sodium polyacrylate with a molecular weight of 10,000 to 30,000 and 300 grams of potassium polyacrylate with a molecular weight of 5,000 to 10,000, stir and dissolve, then add 2,300 grams of zinc oxide that has been ultrafinely crushed and passed through a 1,250-mesh sieve, stir and disperse, then add the mixed solution into a homogenizer with a pressure of 45 MPa, a feed liquid temperature of 45°C, and a homogenization number of 4 times. After homogenization, dry it in a centrifugal drying tower, the air inlet temperature of the centrifugal drying tower is controlled at 200°C, and the air outlet temperature is 100°C. After drying, collect the material, pass it through a 20-100 mesh sieve to obtain zinc oxide.

[0084] Comparative Example 6

[0085] A method for preparing zinc oxide, which differs from Example 2 only in that no water-soluble sugar and water-soluble salt are added (ie, no maltose syrup, hydroxypropyl starch, lactose and sodium citrate are added).

[0086] The details are as follows: take 10,000 grams of water, add 150 grams of ammonium polyacrylate with a molecular weight of 3,000 to 20,000, and 400 grams of potassium polyacrylate with a molecular weight of 5,000 to 10,000, stir and dissolve, then add 2,000 grams of zinc oxide that has been ultrafinely crushed and passed through a 1,250-mesh sieve, stir and disperse, then add the mixed solution into a homogenizer with 55 MPa, a feed liquid temperature of 50°C, and a homogenization number of 4 times. After homogenization, dry it in a centrifugal drying tower, the air inlet temperature of the centrifugal drying tower is controlled at 190°C, and the air outlet temperature is at 95°C. After drying, collect the material, pass it through a 20-100 mesh sieve, and obtain zinc oxide.

[0087] Comparative Example 7

[0088] Water-soluble zinc oxide was prepared by the method in Chinese invention patent CN102659171A (the amount of zinc oxide added during the preparation process was the same as that in Example 1).

[0089] The specific method is as follows: (1) dissolving 2300 g of zinc salt and 2300 g of modifier in 45000 g of organic solvent, and stirring to prepare a transparent solution; (2) pouring the solution obtained in step (1) into a three-necked flask, introducing nitrogen or argon as a protective gas, with a gas flow rate of 1000 to 5000 ml / min, heating to 200 to 320°C, keeping the temperature for 2 hours, and then cooling; centrifuging the reaction solution at 5000 to 15000 rpm to obtain zinc oxide; the obtained zinc oxide is monodisperse granular nanoparticles.

[0090] Comparative Example 8

[0091] A method for preparing zinc oxide, which is different from Example 1 only in that no acrylate is added (ie, no sodium polyacrylate and potassium polyacrylate are added).

[0092] The details are as follows: take 10,000 grams of water, add 300 grams of potassium citrate and 200 grams of glucose and stir to dissolve, then add 2,300 grams of zinc oxide that has been ultrafinely crushed and passed through a 1,250-mesh sieve and stir to disperse, then add the mixed solution into a homogenizer with a pressure of 45 MPa, a liquid temperature of 45°C, and a homogenization number of 4 times. After homogenization, dry it in a centrifugal drying tower. The air inlet temperature of the centrifugal drying tower is controlled at 200°C, and the air outlet temperature is 100°C. After drying, collect the material, pass it through a 20-100 mesh sieve to obtain zinc oxide.

[0093] Comparative Example 9

[0094] A method for preparing zinc oxide, which differs from Example 2 only in that no acrylate is added (ie, no ammonium polyacrylate and potassium polyacrylate are added).

[0095] The specific preparation method is as follows: take 10,000 grams of water, add 100 grams of maltose syrup, 50 grams of hydroxypropyl starch, 50 grams of lactose, and 300 grams of sodium citrate, stir to dissolve, then add 2,000 grams of ultrafinely crushed zinc oxide that has passed through a 1,250-mesh sieve, stir and disperse, then add the mixed solution into a homogenizer with 55 MPa, a feed liquid temperature of 50°C, and a homogenization number of 4 times. After homogenization, dry it in a centrifugal drying tower. The air inlet temperature of the centrifugal drying tower is controlled at 190°C, and the air outlet temperature is at 95°C. After drying, collect the material, pass it through a 20-100 mesh sieve, and obtain water-soluble zinc oxide.

[0096] Comparative Example 10

[0097] Enteric zinc oxide was prepared by the method in Chinese invention patent CN101803684A (the amount of zinc oxide added during the preparation process was the same as that in Example 1).

[0098] The details are as follows: 1) Preparation of granular zinc oxide: 2300 g of zinc oxide, 986 g of microcrystalline cellulose, and 1971 g of 3% polyethylene glycol aqueous solution were weighed and put into the hopper of HWMG200 high-efficiency wet mixing granulator, and the materials were extruded by a low-temperature extruder at a speed of 40 rpm and a pore size of 0.42 mm, and then the oxidative particles were spheronized by a spheronizer with a spheronization disk diameter of 700 mm at a speed of 300 r / min;

[0099] 2) Preparation of coating material: weigh 131 g of polypropylene resin, 131 g of talcum powder, 66 kg of diethyl phthalate, and 2957 g of 95% alcohol; dissolve the polypropylene resin in 95% alcohol, then add talcum powder and diethyl phthalate, and stir evenly;

[0100] 3) The granular zinc oxide obtained in step 1) and the coating material obtained in step 2) are mixed in a weight ratio of 1:0.1 and put into a LBF-200 cyclone fluidized granulation coating machine, and the coating is carried out under the conditions of a wind speed of 30 to 35 MHz, an air inlet temperature of 50° C., and an air outlet temperature of 35° C. The coating time is 6 hours per batch to obtain an enteric zinc oxide product.

[0101] Comparative Example 11

[0102] The coated submicron feed-grade zinc oxide in Chinese invention patent CN102318750A was used (the amount of zinc oxide added during the preparation process was the same as that in Example 1).

[0103] Specifically as follows: a coated submicron feed-grade zinc oxide, comprising the following components by weight: 2300 grams of submicron feed-grade zinc oxide, 671 grams of composite shielding agent; the composite shielding agent comprises the following components by weight: 90 parts of potato starch, 6 parts of ethyl cellulose, 4 parts of anhydrous ethanol, and 15 grams of magnesium stearate. The particle size of the submicron feed-grade zinc oxide is 10 μm. The particle size of the potato starch is 300 μm. It is prepared according to Example 7 in its specification.

[0104] Effect Example

[0105] 1. Water solubility test

[0106] The water solubility of the water-soluble zinc oxide prepared in Examples 1 to 2 and the zinc oxide prepared in Comparative Examples 1 to 6 was measured, as follows:

[0107] The experimental design and grouping were carried out according to Table 1.

[0108] Table 1 Experimental design and grouping

[0109] Group Add a plan Treatment 1 group Add 10g of zinc oxide prepared in Comparative Example 1 of the present invention into 1L of water. Treatment 2 groups Add 10g of zinc oxide prepared in Comparative Example 2 of the present invention into 1L of water. Treatment 3 groups Add 10g of zinc oxide prepared in Comparative Example 3 of the present invention into 1L of water. Treatment 4 groups Add 10g of zinc oxide prepared in Comparative Example 4 of the present invention into 1L of water. Treatment 5 groups Add 10g of zinc oxide prepared in Comparative Example 5 of the present invention into 1L of water. Treatment 6 groups Add 10g of zinc oxide prepared in Comparative Example 6 of the present invention into 1L of water. Treatment 7 groups 10 g of the water-soluble zinc oxide prepared in Example 1 of the present invention was added to 1 L of water. Processing 8 groups 10 g of the water-soluble zinc oxide prepared in Example 1 of the present invention was added to 1 L of water. Processing 9 groups Add 10 g of zinc oxide prepared in Comparative Example 7 of the present invention into 1 L of water.

[0110] Measurement items

[0111] Water solubility rate: Under room temperature, weigh 10g of sample from the treatment group (accurate to 0.01g) and dissolve it in 1L of grade tertiary water. Stir while adding the water. Start timing from the time the sample is added to the water and calculate the time required for the sample to be completely dissolved.

[0112] The results are shown in Table 2. No water-soluble sugar and / or water-soluble salt is added during the preparation process, and the prepared zinc oxide is still insoluble in water. The zinc oxide prepared by the method of Examples 1 to 2 has good water solubility and can be completely dissolved in water in less than 50 seconds. Its effect is better than the zinc oxide prepared by the method in the prior art (Comparative Example 7).

[0113] Table 2 Water solubility results of zinc oxide under different treatments

[0114] Treatment Group Time required for complete dissolution Treatment Group 1 Insoluble Treatment Group 2 Insoluble Treatment Group 3 Insoluble Treatment Group 4 Insoluble Treatment Group 5 Insoluble Treatment Group 6 Insoluble Treatment Group 7 44 seconds Treatment Group 8 47 seconds Treatment Group 9 2 minutes 4 seconds

[0115] 2. Supplementary data on the gastric effect of water-soluble zinc oxide:

[0116] The experimental design is shown in Table 3.

[0117] Table 3 Determination time points of zinc oxide in artificial simulated gastric fluid

[0118]

[0119] Preparation of artificial simulated gastric juice: take 16.4 mL of dilute hydrochloric acid (take 234 mL of hydrochloric acid, add water to 1 000 mL, and mix well), add about 800 mL of water and 10.0 g of pepsin (enzyme activity ≥ 1 200 U / g, CAS: 9001-75-6), stir well, add water to make up to 1000 mL.

[0120] Detection method and sample processing:

[0121] Determine according to the requirements of 5.5.2.3.2 of HG / 2792-2011.

[0122] Weigh about 1 g of the water-soluble zinc oxide sample prepared in Example 1, add it to 100 mL of artificial simulated gastric juice solution, place it in a water bath at 38-38.1° C. and shake it. According to the experimental design time, take out the liquid and determine the zinc oxide according to the provisions of 5.5.2.3.2 of HG / 2792-2011, and calculate the dissolution rate of the water-soluble zinc oxide.

[0123] The results are shown in Table 4. Within 2 hours, the dissolution of water-soluble zinc oxide in artificial simulated gastric juice was very slow, proving that zinc oxide was hardly destroyed by gastric juice.

[0124] Table 4 Dissolution rate of water-soluble zinc oxide at different times (%)

[0125] time 15 30 45 60 90 120 Dissolution rate 0.11％ 0.24％ 0.39％ 0.65％ 0.96％ 1.75％

[0126] 3. Effects of zinc oxide on growth performance and diarrhea in weaned piglets

[0127] The experimental design and grouping were carried out according to Table 5.

[0128] Table 5 Experimental design and grouping

[0129] Group Add a plan Treatment 1 group Add 1300 g / t of zinc oxide prepared in Comparative Example 8 of the present invention to the basic diet Treatment 2 groups Add 1300 g / t of zinc oxide prepared in Comparative Example 9 of the present invention to the basic diet Treatment 3 groups Add 1300 g / t of zinc oxide prepared in Comparative Example 5 of the present invention to the basic diet Treatment 4 groups Add 1300 g / t of zinc oxide prepared in Comparative Example 10 of the present invention to the basic diet Treatment 5 groups Add 1300 g / t of zinc oxide prepared in Comparative Example 7 of the present invention to the basic diet Treatment 6 groups Add 1300 g / t of zinc oxide prepared in Comparative Example 11 of the present invention to the basic diet Treatment 7 groups Add 1300 g / t of the water-soluble zinc oxide prepared in Example 1 of the present invention to the basic diet. Processing 8 groups 3000g / t zinc oxide in the basal diet (uncoated, high dose of water-insoluble zinc oxide added)

[0130] Note: The basic diet composition and nutritional value are shown in Table 6. 80 ppm of doxycycline was added to the basic diet. Coated zinc oxide ≥ 70% (i.e., the water-soluble zinc oxide content of the added treatment group ≥ 70%).

[0131] Table 6 Basic diet

[0132]

[0133]

[0134] Table 7 Ratio of premix

[0135]

[0136] 240 healthy weaned piglets of the same age (about 7.8 kg / pig) were selected and randomly divided into 8 treatments, with 2 replicates in each treatment and 15 piglets in each replicate. According to the experimental design (see Table 5), each group was fed with the corresponding experimental diet for 18 days, and each group was weighed before and after the experiment.

[0137] Feeding and management: During the trial, the daily management of the experimental pig house was carried out according to the regular procedures of the pig farm. Adopt the feeding method of free access to food and free drinking water. Keep the pig house clean and hygienic, and keep good ventilation. During the trial, regularly observe and record the piglet diseases and deaths every day.

[0138] Measurement items:

[0139] (1) Growth performance: The feed intake, number and weight of pigs that died and were culled were recorded every day, and the average daily weight gain, daily feed consumption, feed-to-meat ratio and mortality rate at each stage during the trial were calculated.

[0140] Average daily feed intake = (total amount of added feed - remaining feed in the trough) / (number of remaining pigs in the pen × total number of experimental days + number of dead pigs × number of experimental days);

[0141] Average daily weight gain = (weight at the end of the pen - weight at the beginning of the pen + weight at the end of dead pigs) / (number of pigs remaining in the pen × total number of days of the experiment + number of dead pigs × number of days of the experiment);

[0142] Feed to meat ratio = average daily feed intake / average daily weight gain;

[0143] Mortality rate = (total number of deaths × 100%) / total number of piglets in each group.

[0144] (2) Diarrhea rate: The diarrhea situation of piglets was recorded every day and the diarrhea rate was calculated. Diarrhea rate = total number of diarrhea times × 100% / (total number of pigs tested × total number of days tested).

[0145] (3) Appearance scoring: The pigs are observed and compared once a week. Based on their coat and color, they can be divided into four grades: poor, medium, good, and excellent (with scores of 1, 2, 3, and 4 points respectively).

[0146] Data statistics and analysis: The experimental data were sorted using Excel 2010, SPSS16.0 software was used for one-way analysis of variance, and the Dun-can method was used for significant difference test. The results were expressed as mean ± standard error.

[0147] result:

[0148] It can be seen from Table 8 that the effects of adding zinc oxide treated by different treatment methods to the piglet diet are quite different. Specifically, the average final weight, average feed intake and average daily weight gain of the piglets in treatment group 7 (adding the water-soluble zinc oxide prepared by Example 1 of the present invention) are significantly higher than those in treatment groups 1 to 6, and better than the treatment group adding high-dose zinc oxide; in terms of feed-to-meat ratio, the feed-to-meat ratio of the piglets in treatment group 7 is better than that of the control treatment groups 1 to 6; in terms of diarrhea rate, the diarrhea rate of the piglets in treatment group 7 is the lowest; there is no significant difference in the coat color appearance of the piglets in each treatment group.

[0149] Table 8 Effects of zinc oxide prepared by different methods on the production performance of suckling piglets

[0150]

[0151]

[0152] Note: The letters on the right shoulder of each data in the table represent significance. If the letters are the same, there is no significant difference; if the letters are different, there is a significant difference.

[0153] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge of ordinary technicians in the relevant technical field without departing from the purpose of the present invention. In addition, the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

Claims

1. A gastric coating material comprising acrylate, water-soluble sugar and water-soluble salt.

2. The gastric coating material according to claim 1, characterized in that: The gastric coating material comprises, by weight, 1 to 8 parts of acrylate, 1 to 5 parts of water-soluble sugar and 1 to 6 parts of water-soluble salt.

3. The water-soluble zinc oxide according to claim 1 or 2, characterized in that The acrylate salt includes at least one of sodium acrylate, potassium acrylate, ammonium acrylate, magnesium acrylate, and calcium acrylate with a molecular weight of 3000 to 1000 000; and / or the water-soluble salt includes at least one of sodium citrate, potassium citrate, sodium succinate, sodium chloride, potassium chloride, calcium chloride, potassium phosphate, magnesium phosphate, sodium phosphate, sodium adipate, sodium fumarate, sodium benzoate, and potassium sorbate.

4. The gastric coating material according to claim 1 or 2, characterized in that: The water-soluble sugar includes at least one of monosaccharide, disaccharide and polysaccharide; Preferably, the monosaccharide includes at least one of glucose, mannose, ribose, xylose, arabinose, rhamnose, mannose, galactose and fructose; and / or, the disaccharide includes at least one of sucrose, gentiobiose, maltose, cellobiose, lactose and laminaribiose; and / or, the polysaccharide includes at least one of starch, glycogen and raffinose, and the starch is soluble modified starch.

5. A water-soluble zinc oxide, made from the following raw materials: the gastric coating material according to any one of claims 1 to 4 and zinc oxide.

6. The water-soluble zinc oxide according to claim 5, characterized in that The water-soluble zinc oxide is prepared from the following raw materials, by weight: 3 to 20 parts of gastric coating material and 15 to 30 parts of zinc oxide.

7. The method for preparing the water-soluble zinc oxide according to claim 5 or 6, comprising the following steps: The gastric coating material is dissolved in water, mixed evenly, and mixed with zinc oxide to obtain a mixture; the mixture is homogenized to obtain water-soluble zinc oxide; Preferably, the homogenization conditions are 20-50° C., 20-100 MPa, and the homogenization times are 1 to 5 times.

8. Use of the water-soluble zinc oxide according to claim 5 or 6 in the preparation of a product, wherein the product comprises at least one of a reagent, a kit, a medicine, a feed, a cosmetic, a bactericide, and an aqueous conductive material.

9. A feed comprising the water-soluble zinc oxide according to claim 5 or 6.

10. The feed according to claim 9, characterized in that The feed also includes a basal diet.

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