Traditional Chinese medicine compound for preventing and treating mycotoxin pollution of mutton sheep feed and preparation method of traditional Chinese medicine compound

Abstract

The invention discloses a traditional Chinese medicine compound for preventing and treating mycotoxin poisoning of livestock, which is prepared by uniformly mixing golden larch bark, lightyellow sophora root, milkvetch root, corydalis tuber, giant knotweed rhizome, glucose oxidase and sodium diacetate as raw materials in proportion according to different characteristics of each traditional Chinese medicine, and performing vacuum drying to prepare powder. The formula and process are unique and scientific, and the treatment effect is remarkable.

Description

Technical Field

[0001] The invention belongs to the field of feed additives, and in particular relates to a traditional Chinese medicine compound for preventing and treating mycotoxin contamination in mutton sheep feed and a preparation method thereof. Background Art

[0002] Mycotoxins are an important class of harmful substances in animal feed and food. They are metabolites produced by certain molds (such as Aspergillus, Penicillium and Fusarium) and can be produced in agricultural products such as grains, feed and nuts. According to statistics from the Food and Agriculture Organization of the United Nations (FAO), nearly 25% of forage crops are contaminated with mycotoxins to varying degrees worldwide each year, of which 2% of forage crops will directly lose their economic value due to serious pollution, with losses of up to tens of billions of dollars. In my country, the detection rate of mycotoxins in silage is as high as 70% to 100%, and forage raw materials and hay are contaminated with mycotoxins to varying degrees, with as few as 2 to 3 types detected and as many as 7 to 8 types. In particular, the detection rate in the hot and humid southern region is higher than that in the north. In recent years, the contamination of mycotoxins in forage and compound feed has become more serious, the detection rate remains high, and the rate of exceeding the standard has also tended to rise.

[0003] Mycotoxins can suppress the immune system of animals, causing them to become less resistant to diseases. Mycotoxins may also cause reproductive problems, including infertility and miscarriage. Aflatoxin, a type of mycotoxin, is particularly known for its toxicity to the liver, which can cause liver enlargement and liver dysfunction; while ochratoxin mainly affects the kidneys, leading to renal failure. In addition, the presence of mycotoxins can lead to a reduction in the animal's feed intake, which directly affects its growth rate and production performance. Due to the inhibition of feed digestion and absorption, the animal's growth rate will be significantly reduced, affecting its final weight and meat quality. Mycotoxins not only affect the health and production performance of animals, but also cause significant economic losses. Due to the influence of mycotoxins, animals may require more veterinary care, increasing the economic burden on breeders. In addition, mycotoxins will enter the animal's body with feed, remain in the body and accumulate, causing poisoning and even death in livestock. At the same time, mycotoxins can also enter the human body with meat, eggs, milk, etc., causing serious health hazards such as cancer, deformity, and infertility.

[0004] In modern agricultural production, if the feed is not kept dry and ventilated during storage, or the humidity of the storage environment is too high, the feed is prone to mold. Feed contaminated with mycotoxins is usually discarded or treated harmlessly, which further increases the cost of breeding. However, in some cases, livestock may still accidentally eat moldy or mycotoxin-contaminated feed. On the one hand, feed is usually stored in piles. Although the moldy feed is cleaned up, the non-moldy part may still be contaminated by mycotoxins. On the other hand, when feed is short of supply, farmers may be forced to use moldy feed to ensure that the animals have enough food. Furthermore, some farmers may lack awareness of the hazards of moldy feed and think that as long as the animals are willing to eat it, they can continue to use it.

[0005] Therefore, it is urgent to solve the problem of mycotoxin contamination in feed, reduce production costs and protect livestock health. Summary of the invention

[0006] In order to solve the problems existing in the prior art, the present invention provides a traditional Chinese medicine compound for preventing and treating mycotoxin poisoning in livestock, which has a unique and scientific formula and process and has a significant effect in preventing livestock from being affected by mycotoxins.

[0007] To this end, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention relates to a traditional Chinese medicine compound for preventing and treating mycotoxin contamination in livestock feed, comprising thorn bark, sophora flavescens, astragalus, corydalis, polygonum cuspidatum and an immunomodulator.

[0009] Among them, the Chinese medicinal raw materials are:

[0010] Tujingpi: It is the dried root bark or near-root bark of Pseudolarix kaempferi Gord., a plant of the Pinaceae family. It is pungent and warm. It enters the lung and spleen meridians. It dispels wind and dampness; kills insects and relieves itching.

[0011] Sophora flavescens: It is the dried root of Sophora flavescens Ait. of the Leguminosae family. It tastes bitter and cold. It enters the heart, liver, stomach, large intestine and bladder meridians. It clears away heat and dampness, kills insects and is a diuretic.

[0012] Astragalus: It is the dried root of Astragalus membranaceus (Fisch) Bge. var. mongholicus (Bge) Hsiao or Astragalus membranaceus (Fisch) Bge. of the Leguminosae family. It is sweet and slightly warm. It enters the lung and spleen meridians. It replenishes qi and raises yang, consolidates the exterior and stops sweating, promotes diuresis and reduces swelling, promotes the production of body fluids and nourishes blood, relieves stagnation and arthralgia, removes toxins and discharges pus, and heals sores and promotes tissue regeneration.

[0013] Corydalis edulis Maxim: The whole herb or root of Corydalis edulis Maxim, a plant of the Poppy family. Bitter, astringent, and cool. Enters the lung, kidney, and spleen meridians. Clears away heat and detoxifies; kills insects and relieves itching.

[0014] Polygonum cuspidatum: It is the dried rhizome and root of Polygonum cuspidatum Sieb.et Zucc. of the Polygonaceae family. It tastes slightly bitter and cold. It enters the liver, gallbladder and lung meridians. It can remove dampness and jaundice, clear away heat and detoxify, disperse blood stasis and relieve pain, and relieve cough and resolve phlegm.

[0015] Furthermore, the traditional Chinese medicine compound for preventing and treating mycotoxin contamination in livestock feed comprises 20-45 parts of thorn bark, 20-45 parts of sophora flavescens, 60-90 parts of astragalus, 10-35 parts of corydalis, 20-45 parts of knotweed and 0.5-15 parts of immunomodulator.

[0016] Wherein, the immune additive is selected from one or more of inulin, ginseng, vitamin E, glucose oxidase, sodium butyrate, sodium diacetate, EPA, DHA and lactoferrin.

[0017] Furthermore, the traditional Chinese medicine compound for preventing and treating mycotoxin contamination in livestock feed comprises 30 parts of thorn bark, 30 parts of sophora flavescens, 70 parts of astragalus, 20 parts of corydalis, 30 parts of knotweed and 6 parts of immunomodulator.

[0018] Wherein, the immune additive is selected from glucose oxidase and sodium diacetate; the mass ratio of glucose oxidase to sodium diacetate is 1:5.

[0019] In a second aspect, the present invention relates to a traditional Chinese medicine compound for preventing and treating mycotoxin contamination in livestock feed, which is prepared from 30 parts of thorn bark, 30 parts of sophora flavescens, 70 parts of astragalus, 20 parts of corydalis, 30 parts of knotweed, 1 part of glucose oxidase and 5 parts of sodium diacetate.

[0020] In a third aspect, the present invention relates to a method for preparing a traditional Chinese medicine compound for preventing and treating mycotoxin contamination in livestock feed, characterized in that the method comprises the following steps:

[0021] ① According to the formula ratio described in any of the above aspects, weigh the bark of thorns, sophora flavescens, astragalus, corydalis and polygonum cuspidatum, vacuum dry them, crush them, sieve them, and obtain a Chinese medicine raw material powder for standby use;

[0022] ② Weigh the immunomodulator according to the formula ratio, sieve it, and obtain the immunomodulator powder for later use;

[0023] ③Mix the Chinese medicine raw material powder and the immunomodulator powder, stir evenly and sieve to obtain the Chinese medicine complex.

[0024] In a fourth aspect, the present invention relates to a Chinese medicine complex for preventing and treating mycotoxin contamination in livestock feed as described in any one of the first aspect or the second aspect, or a use of the Chinese medicine complex prepared by the preparation method described in the third aspect of the present invention in preparing a feed additive for preventing and treating mycotoxin contamination in livestock feed.

[0025] Further, wherein the livestock is a mammal;

[0026] Preferably, the livestock is a ruminant;

[0027] Preferably, the livestock are cattle, sheep or horses.

[0028] Furthermore, the feed additive can inhibit the reduction of feed intake, decreased immunity and inflammatory response of livestock caused by mycotoxin contamination.

[0029] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0030] The invention uses Chinese medicinal materials of thorn bark, sophora flavescens, astragalus, corydalis, and polygonum cuspidatum as raw materials and immunomodulators of glucose oxidase and sodium diacetate as raw materials, and reasonably matches the raw materials with different characteristics, so as to effectively prevent livestock from having reduced feed intake, decreased immunity, and inflammatory reactions due to eating moldy feed by mistake, increase the feed intake of livestock, promote the improvement of livestock production performance, and prevent livestock from getting sick. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 .Effects of different drug formulations on ZEA toxicity

[0032] Figure 2 .Mycotoxin content in each group of formulas

[0033] Figure 3 .Effects of each group of formula on livestock growth performance

[0034] Figure 4 .Effects of each group of formula on livestock immunoglobulin

[0035] Figure 5 .Effects of each group of formula on livestock cytokines DETAILED DESCRIPTION

[0036] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The raw materials, reagents or devices used in the following examples, unless otherwise specified, can be obtained from conventional commercial sources, or can be obtained by existing known methods. The experimental methods without specifying specific conditions are usually based on conventional conditions or the conditions recommended by the manufacturer. Unless otherwise stated, parts and percentages are parts by weight and weight percentages.

[0037] Example 1 Optimization and preparation of traditional Chinese medicine compound formula

[0038] 1. Experimental samples

[0039] Dry each Chinese medicine raw material in a drying oven, take 10g of each single Chinese medicine, add 8 times the volume of the weighed Chinese medicine to soak for 2h, boil over high heat, continue to simmer for 30min, filter with gauze. Set aside the filtrate, add 5 times the volume of sterilized water to the filter residue, boil it, simmer for 20min, combine the two filtrates, vacuum dry it and keep it as a solid for later use.

[0040] Zea mays erythroenone (ZEA) was dissolved in DMSO to prepare a 0.1 M ZEA solution, which was then aliquoted and stored at -20°C in the dark for later use. It was diluted with PBS to the desired concentration before use.

[0041] Table 1 Experimental drug formulation

[0042] Raw material mg / mL Experimental Group 1 Experimental Group 2 Experimental Group 3 Experimental Group 4 Experimental Group 5 Experimental Group 6 Psoralea corylifolia 30 30 90 30 30 30 Sophora flavescens 30 30 90 30 30 30 Astragalus 70 70 / / 70 70 Corydalis 20 20 / 20 20 / Knotweed 30 30 / 30 / 30 honeysuckle / / / / / 20 Artemisia capillaris / / / / 30 / Codonopsis pilosula / / / 70 / / Glucose oxidase 1 / 3 1 1 1 Sodium diacetate 5 / 3 5 5 5 Lactoferrin / 1 / / / / Sodium Butyrate / 5 / / / /

[0043] According to the formula in Table 1, the required mass of each raw material was weighed and dissolved with PBS. The final concentration of each raw material in the solution was shown in Table 1 to prepare a mother solution. The prepared mother solution of each experimental group was diluted with PBS to 1 / 100 of the mother solution before adding to the cell plate and filtered with a 0.22 μm filter to remove bacteria and pyrogens.

[0044] 2. Experimental Methods

[0045] MTT method:

[0046] Sheep lung fibroblast (primary immortalized) cells OAR-L1 were cultured in DMEM medium (containing 10% FBS, 1% PS) at 5% CO2 and 37°C;

[0047] Collect cells growing in the logarithmic phase, adjust the concentration of the cell suspension, inoculate the cell suspension in a 96-well plate, and plate the cells to be tested so that the density is adjusted to about 1×10 4 / well, 100 μL cell suspension per well, incubate at 5% CO2, 37°C for 24h, observe under microscope that cells adhere to the wall and grow, add 10 μL ZEA to the culture plate, where the final concentration of ZEA is 100 μM. After incubation for 1h, add 10 μL of the drug solution of experimental groups 1 to 6 to each well (so that the final concentration of the drug solution added to the cells is μg / mL), and finally add PBS as a blank control. The culture plate was placed in a 5% CO2, 37°C constant temperature incubator for culture. After 24h, take out the culture plate, add 10 μl of MTT (5mg / mL) solution to each well, continue to culture for 4h, terminate the culture, and carefully absorb the culture solution in the well. Then add 100 μl DMSO to each well, place it under a shaker and oscillate at a low speed for 10min to fully dissolve the purple crystals. Detect the absorbance value of each well at 490nm on an automatic microplate reader.

[0048] 3. Experimental results

[0049] The experimental results are as follows Figure 1 shown.

[0050] The results showed that ZEA could induce OAR-L1 death, and the drug in experimental group 1 could significantly protect OAR-L1 cells from cell death caused by ZEA. Experimental group 2 replaced the immunomodulation in experimental group 1, and the cell protection effect disappeared. Experimental group 3 only retained the bark of thorns, sophora flavescens glucose oxidase and sodium diacetate, and changed the raw material dosage ratio, and the cell protection effect disappeared. Experimental group 4 used Codonopsis pilosula, which is also a qi-invigorating drug, to replace Astragalus, experimental group 5 used Artemisia capillaris, which is also a diuretic drug, to replace Polygonum cuspidatum, and experimental group 6 used Honeysuckle, which is also a heat-clearing and detoxifying drug, to replace Corydalis. However, the results showed that the use of similar medicinal flavors with similar efficacy and medicinal properties caused the cell protection effect of experimental group 1 to disappear. It can be seen that the medicinal flavors and dosage ratios selected by the present invention have unexpected technical effects.

[0051] Example 2 Verification of the effect of Chinese herbal medicine compound in preventing and treating mycotoxin poisoning in livestock

[0052] The Chinese medicine compound used for verification was prepared by the following method:

[0053] ① Weigh 30 parts of Radix Chaenomelis, 30 parts of Radix Sophorae Flavescentis, 70 parts of Radix Astragali, 20 parts of Rhizoma Corydalis and 30 parts of Polygonum Cuspidati, vacuum dry and crush, sieve to obtain Chinese medicine raw material powder, and set aside;

[0054] ② Weigh 1 part of glucose oxidase and 5 parts of sodium diacetate, sieve to obtain immunomodulator powder, and set aside;

[0055] ③Mix the Chinese medicine raw material powder and the immunomodulator powder, stir evenly and sieve to obtain the Chinese medicine complex.

[0056] 1. Experimental Protocol

[0057] The feeds for each group were prepared according to the formula in Table 2.

[0058] Table 2 Feed test formula

[0059] Raw materials / mass Blank control group Model control group Chinese medicine compound group corn 555 290 282 Pit material + moldy corn / 265 260 Second powder 20 20 20 Corn Germ Oil 200 200 196 Soybean meal 46% 145 145 142 Slow-release urea (200%) 5 5 5 Molasses 15 15 15 Stone powder 10 10 10 Calcium Hydrogen Phosphate 5 5 5 Sodium chloride 10 10 10 Soybean Oil 10 10 10 Premix (Z600) 25 25 25 Chinese herbal medicine complex / / 20 total 1000 1000 1000

[0060] 2. Experimental Grouping

[0061] The experiment was divided into blank control group, model control group and Chinese medicine compound group.

[0062] Blank control group: fed with the blank control group formula in Table 1.

[0063] Model control group: The rats were fed the model control group formula in Table 1, in which part of the moldy corn was substituted for normal corn.

[0064] Chinese medicine complex group: The rats were fed the Chinese medicine complex group formula in Table 1, in which part of the moldy corn was used to replace the normal corn, and the prepared Chinese medicine complex was added.

[0065] 3. Experimental Design

[0066] The animal experiment used 8-month-old female Hu sheep from Inner Mongolia Shengle Animal Husbandry, with an average weight of 32.80 kg. The experimental animals were randomly divided into a blank control group (n=10), a model control group (n=10) and a Chinese medicine compound group (n=10), and were fed with the blank control group formula in Table 1, the model control group formula in Table 1 and the Chinese medicine compound group formula in Table 1, respectively. The experimental period was 90 days. Before the feeding experiment, the animals went through a 10-day adaptation period to obtain an appropriate amount of feed intake. During the experiment, the animals could eat and drink freely.

[0067] 4. Weighing and Sample Collection

[0068] Before the start of the experiment, all sheep were weighed by an automatic weighing system to obtain their initial body weight. The body weight on the 90th day was then weighed to calculate the daily weight gain. The remaining feed intake was collected every day to calculate the feed intake of the mutton sheep. Blood was collected from the jugular vein on an empty stomach and placed in a 5mL vacuum negative pressure tube. It was centrifuged at 3000rpm for 8min, and the supernatant was taken and immediately stored in a -80℃ refrigerator and sent to the laboratory for further analysis. At the end of the trial period, 10 Hu sheep in each group were slaughtered and tissue samples were collected. Within 20 minutes after slaughter, the longissimus dorsi muscle on the left side of the carcass and subcutaneous fat tissue samples (approximately 0.5×0.5×0.5cm 3 ), placed in 2 mL cryovials, and immediately frozen in liquid nitrogen for subsequent analysis.

[0069] 5. Detection indicators

[0070] 5.1 Mycotoxin content in each group of formulas

[0071] The UPLC-QQQ-MS method was established for quantitative analysis, and the results were as follows Figure 2 As shown in the figure, in the model control group feed that replaced normal corn with moldy corn, the representative mycotoxins exceeded the standard significantly, among which aflatoxin B1 (AFB1) was 4.5ppb, about 5 times that of the blank group, the content of vomitoxin (DON) was 2 times that of the blank group, and the content of zeaxenone (ZEA) was 7-8 times that of the blank group. The above results indicate that the modeling of this experiment was successful. We used the formula of the model control group as the basis and added the Chinese medicine compound for intervention treatment.

[0072] 5.2 Growth performance

[0073] Before and at the end of the formal experiment, the experimental sheep were fasted for 12 hours and weighed as the initial weight and final weight to calculate the average daily weight gain. During the experiment, the feeding amount and residual feed amount of each repetition were accurately recorded every day to calculate the daily feed intake. The feed-to-weight ratio was calculated from the average daily feed intake and the average daily weight gain.

[0074] Average daily weight gain = (average weight at the end of the test - average weight at the beginning of the test) / number of test days × number of heads in each group

[0075] Average daily feed intake = total feed consumption per group / (number of test days × number of heads per group)

[0076] Feed to weight ratio = average daily feed intake / average daily weight gain

[0077] 5.3 Blood immune indicators

[0078] Before morning feeding on the day when the formal experiment ended, 10 mL of blood was collected from the subjugular vein of the sheep using a vacuum blood collection tube, centrifuged at 3000 rmin for 15 min, and the upper serum was aspirated, divided into 2 mL centrifuge tubes and marked, and stored in a -20 ° C refrigerator. The serum immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), interleukin-6 (IL-6), interleukin-10 (IL-10), and tumor necrosis factor (TNF-α) were tested according to the immune index kit and automatic biochemical analyzer of Nanjing Jiancheng Reagent Co., Ltd.

[0079] 6. Experimental Results

[0080] 6.1 Growth performance

[0081] like Figure 3 As shown in A., the initial weight of each group of Hu sheep was similar, with an average initial weight of 32.1±0.6kg. After 100 days of feeding treatment, the average weight of Hu sheep in the model control group decreased by 4.59kg, a decrease of 10.9%; compared with the model control group, the weight of the Chinese medicine complex group increased by 4.76kg, an increase of 12.8% (see the results Figure 3B). Compared with the blank control group, the average daily feed intake of the model control group decreased by 49.26g, a decrease of 6.35%. Compared with the model control group, the average daily feed intake of the Chinese medicine compound group increased by 19.6g, an increase of 2.69% (see the results Figure 3 C). The average daily weight gain of the Chinese medicine complex was significantly increased (see the results in Figure 3 D.).

[0082] The above experimental results show that compared with the model control group, the Chinese medicine compound involved in the present invention significantly increased the final body weight and average daily weight gain of Hu sheep, indicating that the Chinese medicine compound in the present invention can effectively improve the growth performance of Hu sheep, thereby reducing feeding costs.

[0083] 6.2 Blood immune indicators

[0084] IgM, IgA and IgG are close indicators of animal immunity. The decline of Huyang's immunity will cause pathogens to invade and affect the health of Huyang. IgM plays an important role in binding with antigens and hemolysis, IgA can resist virus invasion and kill foreign viruses, and IgG can regulate immune phagocytes and kill viruses.

[0085] like Figure 4 As shown in the figure, the IgM, IgA and IgG of the model control group were significantly increased, indicating that feeding feed contaminated with mycotoxins would lead to a significant decrease in the immunity of Hu sheep. Compared with the model control group, the serum IgM, IgA and IgG levels of Hu sheep in the group fed with the Chinese herbal compound decreased significantly, indicating that the immunity of Hu sheep fed with the Chinese herbal compound was significantly higher than that of the model group, thus enhancing the immunity of Hu sheep.

[0086] Low levels of IL-10 often coexist with high levels of the pro-inflammatory cytokines TNF-α and IL-6, suggesting an unbalanced immune state.

[0087] like Figure 5 As shown in the figure, the TNF-α and IL-6 of the Hu sheep in the model control group increased significantly, and IL-10 decreased significantly, indicating that feeding feed contaminated with mycotoxins would lead to active inflammatory response and immune imbalance in Hu sheep. Compared with the model control group, the serum TNF-α and IL-6 of the Hu sheep in the group fed with the Chinese medicine compound decreased significantly, while IL-10 increased significantly, indicating that feeding the Chinese medicine compound can effectively improve the inflammatory response and immune imbalance caused by mycotoxin contamination, reduce inflammatory response and enhance immunity.

[0088] In summary, the Chinese medicine complex described in the present invention can effectively protect mutton cells from cell death caused by ZEA, and has a significant cell protection effect. The Chinese medicine complex is used as a feed additive and added to moldy feed, which can significantly reduce the negative impact of moldy feed on the production performance of mutton sheep such as feeding and weight gain. At the same time, it can also significantly enhance the immunity of livestock, and significantly inhibit the inflammatory response caused by livestock accidentally eating moldy feed. It can be seen that the Chinese medicine complex provided by the present invention can effectively avoid various adverse reactions caused by livestock accidentally eating moldy feed during the production process, greatly reducing the feeding costs of farmers, and has broad prospects for economic and social benefits.

[0089] Special note: Instructions for use with drawings Figure 2 , Figure 3 , Figure 4 and Figure 5 The Chinese herbal medicine additive group is equivalent to the Chinese medicine compound group in the specification of the present invention.

[0090] The above contents are further detailed descriptions of the present invention in combination with specific preferred embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the protection scope of the present invention.

Claims

1. A Chinese medicinal compound for preventing and treating mycotoxin contamination of livestock feed, comprising thorn bark, sophora flavescens, astragalus, corydalis, polygonum cuspidatum and an immunomodulator.

2. The Chinese medicine complex according to claim 1, comprising 20-45 parts of thorn bark, 20-45 parts of sophora flavescens, 60-90 parts of astragalus, 10-35 parts of corydalis, 20-45 parts of knotweed and 0.5-15 parts of immunomodulator.

3. The traditional Chinese medicine complex according to claim 1 or 2, wherein the immune additive is selected from one or more of inulin, ginseng, vitamin E, glucose oxidase, sodium butyrate, sodium diacetate, EPA, DHA and lactoferrin.

4. The Chinese medicine complex according to any one of claims 1 to 3, comprising 30 parts of thorn bark, 30 parts of sophora flavescens, 70 parts of astragalus, 20 parts of corydalis, 30 parts of knotweed and 6 parts of immunomodulator.

5. The Chinese medicine complex according to any one of claims 1-4, wherein the immune additive is selected from glucose oxidase and sodium diacetate; the mass ratio of glucose oxidase to sodium diacetate is 1:

5.

6. The Chinese medicine composite according to any one of claims 1-5, which is prepared from 30 parts of wormwood bark, 30 parts of sophora flavescens, 70 parts of astragalus, 20 parts of corydalis, 30 parts of knotweed, 1 part of glucose oxidase and 5 parts of sodium diacetate.

7. The method for preparing the traditional Chinese medicine complex according to any one of claims 1 to 6, characterized in that: The following steps are involved: ① Weigh the bark of thorny thorn, sophora flavescens, astragalus, corydalis and polygonum cuspidatum according to the formula ratio, vacuum dry, crush and sieve to obtain a Chinese medicine raw material powder for standby use; ② Weigh the immunomodulator according to the formula ratio, sieve it, and obtain the immunomodulator powder for later use; ③Mix the Chinese medicine raw material powder and the immunomodulator powder, stir evenly and sieve to obtain the Chinese medicine complex.

8. Use of the Chinese medicinal compound according to any one of claims 1 to 6 or the Chinese medicinal compound prepared according to claim 7 in the preparation of a feed additive for preventing and controlling mycotoxin contamination in livestock feed.

9. The use according to claim 8, wherein the livestock is a mammal; Preferably, the livestock is a ruminant; Preferably, the livestock are cattle, sheep or horses.

10. The use according to claim 8 or 9, wherein the feed additive can inhibit the reduction of livestock feed intake, decreased immunity and inflammatory response caused by mycotoxin contamination.

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