Composite peptide dietary supplement for improving specific endowment constitution as well as preparation method, preparation and application of composite peptide dietary supplement

Abstract

The invention provides a composite peptide dietary supplement for improving specific endowment constitution as well as a preparation method, a preparation and application of the composite peptide dietary supplement, and belongs to the technical field of food biology. On the first aspect, the invention provides the composite peptide dietary supplement, and the composite peptide dietary supplement comprises the following raw materials: sea-buckthorn, wrinkled gianthyssop herb, perilla frutescens, oat, smoked plum, heartleaf houttuynia herb, mint, radix angelicae, gordon euryale seed, red date, lactitol, isomaltooligosacharide and liquorice; on the second aspect, the invention provides a preparation method of the composite peptide dietary supplement; and thirdly, the invention provides a preparation of the composite peptide dietary supplement. In the fourth aspect, the invention also provides application of the composite peptide dietary supplement and the preparation thereof in preparation of food or medicines suitable for people with specific endowment constitution.

Description

Technical Field

[0001] The present invention relates to the field of food biotechnology, and in particular to a composite peptide dietary supplement for improving special constitutions, and a preparation method, preparation, and application thereof. Background Art

[0002] With the progress of human society, world medicine is changing from disease medicine to "human" medicine. From health to sub-health and then to disease, the influence of physical factors cannot be ignored. Various physical biases are the inherent basis for the occurrence of diseases. At the same time, it is precisely because of the differences in physical constitution that the occurrence and outcome of body diseases are also different. Human physique is both stable and variable. Through intervention, the imbalanced state of human physique can be improved and adjusted, thereby restoring health. Diet for body identification and improvement of biased physique can not only adjust the sub-health state and block its transformation to the disease state, but also achieve the health care effect of active prevention.

[0003] Constitution in traditional Chinese medicine refers to the comprehensive and relatively stable characteristics of an individual in terms of morphological structure, physiological function and psychological state formed during the life process of birth, growth, maturity and aging, which are manifested in individual differences in structure, function, metabolism and response to external stimuli, susceptibility to certain causes and diseases, and certain tendencies in the transmission and progression of diseases. Epidemiological surveys have found that special constitution is a common biased constitution among the nine types of constitution.

[0004] There are three types of special constitutions: the first is allergic constitution, which includes people with allergic rhinitis, allergic asthma, allergic purpura, eczema, urticaria and other allergic diseases. The second is genetic disease constitution, which includes people with a family history of genetic diseases or congenital diseases. This type is difficult to cure. The third is fetal constitution, which is a constitution caused by the adverse effects of the mother during pregnancy being transmitted to the fetus.

[0005] People with special constitutions have poor adaptability to the outside world. For example, people with allergies have poor adaptability to allergy seasons, which easily triggers old illnesses. In addition, some people with allergies often have stuffy noses, sneezing, and runny noses even if they don't have a cold. They are prone to asthma. Some are prone to allergies to drugs, food, smells, pollen, and seasons. Some are prone to urticaria on their skin. Purple-red spots and bruises often appear on the skin due to allergies. The skin often turns red and scratches appear when scratched.

[0006] The constitution theory believes that "physique and disease are related". Constitution is not only an internal factor in the occurrence of disease, but also affects the development and outcome of the disease.

[0007] At present, no candy health food for people with special constitutions using seabuckthorn, Patchouli, etc. as raw materials has been found in the market. In view of this, the present invention formulates a formula according to the standard of "Classification and Determination of Constitution in Traditional Chinese Medicine" officially released by the Chinese Association of Traditional Chinese Medicine on April 9, 2009, selects raw materials that are both medicinal and edible, and adopts scientific technology to make concentrated small molecule peptide tablet candy. The candy can achieve the effect of regulating the sub-health state of people with special constitutions.

[0008] Therefore, the present invention provides a dietary supplement of seabuckthorn, patchouli, perilla, oat, ebony, houttuynia, mint, angelica dahurica, euryale ferox, red dates, lactitol, isomaltooligosaccharide and licorice composite peptide, and a preparation method, preparation and application thereof. Summary of the invention

[0009] The object of the present invention is to provide a composite peptide dietary supplement for improving special constitution and a preparation method, preparation and application thereof.

[0010] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0011] In a first aspect, the present invention provides a composite peptide dietary supplement for improving a special constitution. The raw materials of the composite peptide dietary supplement include seabuckthorn, patchouli, perilla, oats, ebony, houttuynia cordata, mint, angelica dahurica, Euryale ferox, red dates, lactitol, and isomaltooligosaccharide.

[0012] Furthermore, the raw material of the composite peptide dietary supplement also includes liquorice.

[0013] Furthermore, the raw materials of the composite peptide dietary supplement are, by weight, 9-15 parts of seabuckthorn, 2-10 parts of patchouli, 2-10 parts of perilla, 2-8 parts of oats, 2-8 parts of black plums, 2-8 parts of houttuynia cordata, 2-8 parts of mint, 2-8 parts of angelica dahurica, 2-8 parts of Euryale ferox, 2-8 parts of red dates, 1-3 parts of lactitol, 1-3 parts of isomaltooligosaccharides, and 2-8 parts of licorice.

[0014] Furthermore, the raw materials of the composite peptide dietary supplement are, by weight, 12 parts of seabuckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of ebony, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of euryale, 6 parts of red dates, 2 parts of lactitol, 2 parts of isomaltooligosaccharide, and 6 parts of licorice; or 10 parts of seabuckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of ebony, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of euryale, 2 parts of red dates, 2 parts of lactitol, 2 parts of isomaltooligosaccharide, and 4 parts of licorice.

[0015] In a second aspect, the present invention provides a method for preparing a composite peptide dietary supplement for improving a special constitution, comprising:

[0016] The method comprises the following steps: the first step is to prepare the original pulp; the second step is to prepare the fermentation agent; the third step is to prepare the diluted mixed pulp; the fourth step is to prepare the fermentation liquid; the fifth step is the yeast autolysis and ultrasonic wall breaking; the sixth step is the ultrafiltration; the seventh step is the purification; and the eighth step is the drying.

[0017] Furthermore, the specific process of preparing the raw pulp in the first step is to take sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica dahurica, Euryale ferox, red dates, licorice and other raw materials, wash them separately, soak them until there is no dry core, drain the surface water, crush them into raw pulp, and set aside.

[0018] Furthermore, the specific process of preparing the yeast agent in the second step is to select high-activity, high-temperature-resistant and high-activity yeast for inoculation and culture to form a binary yeast liquid A, and to culture Lactobacillus acidophilus to form a yeast liquid B for standby use.

[0019] Furthermore, the specific process for preparing the diluted mixed slurry in the third step is to add lactitol, isomaltooligosaccharide, an appropriate amount of purified water, and an appropriate amount of brown sugar into the fermentation tank according to the weight of the original slurry obtained in the first step, boil it for a period of time, stop heating, cool it down, and obtain the diluted mixed slurry for standby use.

[0020] Furthermore, the specific process for preparing the fermentation liquid in the fourth step is to inoculate the binary yeast liquid A and yeast liquid B in the second step into the diluted mixed slurry obtained in the third step at a ratio of 0.02 to 0.06 times by weight.

[0021] Furthermore, the specific process of the fifth step of yeast autolysis and ultrasonic cell wall breaking is to control appropriate yeast autolysis conditions after terminating the fermentation, autolyze the yeast in the fermentation broth and use ultrasonic cell wall breaking extraction technology to obtain rich yeast small molecule peptides and amino acids.

[0022] Furthermore, the specific process of the sixth step ultrafiltration is to take the supernatant and use an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons for membrane separation to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0023] Furthermore, the specific process of the seventh step of purification is purification using anion exchange resin TEHUAMP-600.

[0024] Furthermore, the specific process of the eighth step of drying is to freeze-dry the purified product to obtain the active polypeptide or spray-dry it.

[0025] Furthermore, in the second step, the high-activity yeast and the high-temperature-resistant high-activity yeast are inoculated into a culture medium at a temperature of 29 to 33° C., cultured at 160 rpm for 12 to 36 hours to prepare a binary yeast solution A; and Lactobacillus acidophilus is inoculated into a culture medium at a temperature of 33 to 37° C., cultured for 36 to 60 hours to prepare a yeast solution B.

[0026] Furthermore, in the second step, the culture medium is yeast extract powder, brown sugar and potato.

[0027] Furthermore, in the third step, based on the weight of the raw pulp obtained in the first step, lactitol, isomaltooligosaccharide, 2 to 5 times of purified water, and 0.1 to 0.2 times of brown sugar are added to the raw pulp in the fermentation tank, and the mixture is boiled for 5 minutes, and the heating is stopped and the mixture is cooled to obtain a diluted mixed pulp for standby use.

[0028] Furthermore, in the fourth step, the fermentation time is 1 to 2 days.

[0029] In a third aspect, the present invention provides a preparation of the composite peptide dietary supplement, which can be a tablet, an granule or an oral solution.

[0030] In a fourth aspect, the present invention also provides a use of the composite peptide dietary supplement and the preparation thereof in preparing food or medicine suitable for people with special constitutions.

[0031] Compared with the prior art, the beneficial effects of the present invention include:

[0032] Compared with the prior art, the composite peptide dietary supplement provided by the present invention has a more scientific and reasonable formula, a high efficiency and low cost preparation method, high activity and high yield of active peptides, high bioavailability of the preparation, and a significant effect on improving special constitution, with significant improvements in many aspects. DETAILED DESCRIPTION

[0033] The embodiments of the present invention will be described in detail below in conjunction with the examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present invention and should not be construed as limiting the scope of the present invention. If specific conditions are not specified in the examples, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0034] The features and performance of the present invention are further described in detail below in conjunction with the embodiments:

[0035] Example 1

[0036] The first step is to prepare the stock solution: take 12 parts of sea buckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plum, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 6 parts of red dates, 2 parts of lactitol, and 2 parts of isomaltooligosaccharide. Wash sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, and red dates separately, soak until there is no dry core, drain the surface water, crush into stock solution, and set aside.

[0037] The second step is to prepare the yeast agent: the culture medium is 2% yeast extract powder, 50% water, 9% brown sugar and 39% potato, and activated high-temperature resistant and highly active yeast is selected, the inoculation amount is 2%, 29°C, 160 rpm, and aeration culture is carried out for 36 hours to make yeast solution A for standby use; the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% potato, and activated Lactobacillus acidophilus is selected, the inoculation amount is 2%, and closed culture is carried out at 33°C for 60 hours to make binary yeast solution B for standby use.

[0038] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add lactitol, isomaltooligosaccharide, 3.5 times purified water, and 0.15 times brown sugar into the fermentation tank, boil for 5 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0039] The fourth step is to prepare the fermentation liquid: the binary yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight respectively, the fermentation temperature is 33° C., and the fermentation time is 36 hours.

[0040] The fifth step is yeast autolysis and ultrasonic cell wall breaking: after the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, the yeast autolysis conditions are controlled at 55°C, 24h, and then the ultrasonic cell wall breaking process is adopted, ultrasonic breaking for 1min, rest for 1min, and breaking 20 times.

[0041] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0042] The seventh step is purification: purification using anion exchange resin TEHUAMP-600.

[0043] Step 8: Drying: The purified product is spray dried.

[0044] Example 2

[0045] The first step is to prepare the stock solution: take 15 parts of sea buckthorn, 2 parts of patchouli, 10 parts of perilla, 2 parts of oats, 8 parts of black plum, 2 parts of houttuynia, 8 parts of mint, 2 parts of angelica, 8 parts of Euryale, 2 parts of red dates, 3 parts of lactitol, 1 part of isomaltooligosaccharide, and 8 parts of licorice. Wash sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, red dates, and licorice separately, soak until there is no dry core, drain the surface water, and crush into stock solution for standby use.

[0046] The second step is to prepare the yeast agent: the culture medium is 2% yeast extract powder, 50% water, 9% brown sugar and 39% potato, and activated high-temperature resistant and highly active yeast is selected, the inoculation amount is 2%, 33°C, 160 rpm, and aeration culture is carried out for 12 hours to make yeast solution A for standby use; the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% potato, and activated Lactobacillus acidophilus is selected, the inoculation amount is 2%, and closed culture is carried out at 37°C for 36 hours to make binary yeast solution B for standby use.

[0047] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add lactitol, isomaltooligosaccharide, 5 times purified water, and 0.1 times brown sugar into the fermentation tank, boil for 7 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0048] The fourth step is to prepare the fermentation liquid: inoculate the binary yeast liquid A and yeast liquid B in the second step into the diluted mixed slurry obtained in the third step at a ratio of 0.03 times by weight, respectively, at a fermentation temperature of 36° C. and a fermentation time of 24 hours.

[0049] The fifth step is yeast autolysis and ultrasonic cell wall breaking: after the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, the yeast autolysis conditions are controlled at 57°C for 12 hours, and then the ultrasonic cell wall breaking process is adopted, ultrasonic breaking for 1.5 minutes, rest for 2 minutes, and breaking for 10 times.

[0050] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0051] The seventh step is purification: purification using anion exchange resin TEHUAMP-600.

[0052] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0053] Example 3

[0054] The first step is to prepare the stock solution: 9 parts of seabuckthorn, 10 parts of patchouli, 2 parts of perilla, 8 parts of oats, 2 parts of black plum, 8 parts of houttuynia, 2 parts of mint, 8 parts of angelica, 2 parts of Euryale, 8 parts of red dates, 1 part of lactitol, 3 parts of isomaltooligosaccharide, and 2 parts of licorice. Wash seabuckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, red dates, and licorice separately, soak until there is no dry core, drain the surface water, and crush into stock solution for standby use.

[0055] The second step is to prepare the yeast agent: the culture medium is 2% yeast extract powder, 50% water, 9% brown sugar and 39% potato, and activated high-temperature resistant and highly active yeast is selected, the inoculation amount is 2%, 29°C, 160 rpm, and aeration culture is carried out for 36 hours to make yeast solution A for standby use; the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% potato, and activated Lactobacillus acidophilus is selected, the inoculation amount is 2%, and closed culture is carried out at 33°C for 60 hours to make binary yeast solution B for standby use.

[0056] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add lactitol, isomaltooligosaccharide, 2 times of pure water, and 0.2 times of brown sugar into the fermentation tank, boil for 3 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0057] The fourth step is to prepare the fermentation liquid: the binary yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.05 times by weight, respectively. The fermentation temperature is 30° C. and the fermentation time is 48 hours.

[0058] The fifth step is yeast autolysis and ultrasonic cell wall breaking: after the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, the yeast autolysis conditions are controlled at 53°C and 36h, and then the ultrasonic cell wall breaking process is adopted, ultrasonic breaking for 0.5min, rest for 0.5min, and breaking for 30 times.

[0059] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0060] The seventh step is purification: purification using anion exchange resin TEHUAMP-600.

[0061] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0062] Example 4

[0063] The first step is to prepare the stock solution: take 10 parts of sea buckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plum, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 2 parts of red dates, 2 parts of lactitol, 2 parts of isomaltooligosaccharide, and 4 parts of licorice. Wash sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, red dates, and licorice separately, soak until there is no dry core, drain the surface water, and crush into stock solution for standby use.

[0064] The second step is to prepare the yeast agent: the culture medium is 2% yeast extract powder, 50% water, 9% brown sugar and 39% potato, and activated high-temperature resistant and highly active yeast is selected, the inoculation amount is 2%, 33°C, 160 rpm, and aeration culture is carried out for 12 hours to make yeast solution A for standby use; the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% potato, and activated Lactobacillus acidophilus is selected, the inoculation amount is 2%, and closed culture is carried out at 37°C for 36 hours to make binary yeast solution B for standby use.

[0065] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add lactitol, isomaltooligosaccharide, 3.5 times purified water, and 0.15 times brown sugar into the fermentation tank, boil for 5 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0066] The fourth step is to prepare the fermentation liquid: the binary yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight respectively, the fermentation temperature is 33° C., and the fermentation time is 36 hours.

[0067] The fifth step is yeast autolysis and ultrasonic cell wall breaking: after the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, the yeast autolysis conditions are controlled at 55°C, 24h, and then the ultrasonic cell wall breaking process is adopted, ultrasonic breaking for 1min, rest for 1min, and breaking 20 times.

[0068] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0069] The seventh step is purification: purification using anion exchange resin TEHUAMP-600.

[0070] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0071] Example 5

[0072] The first step is to prepare the stock solution: take 12 parts of sea buckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plum, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 2 parts of red dates, 2 parts of lactitol, 2 parts of isomaltooligosaccharide, and 6 parts of licorice. Wash sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, red dates, and licorice separately, soak until there is no dry core, drain the surface water, and crush into stock solution for standby use.

[0073] The second step is to prepare the yeast agent: the culture medium is 2% yeast extract powder, 50% water, 9% brown sugar and 39% potato, and activated high-temperature resistant and highly active yeast is selected, the inoculation amount is 2%, 31°C, 160 rpm, and aeration culture is carried out for 24 hours to prepare yeast solution A for standby use; the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% potato, and activated Lactobacillus acidophilus is selected, the inoculation amount is 2%, and closed culture is carried out at 35°C for 48 hours to prepare binary yeast solution B for standby use.

[0074] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add lactitol, isomaltooligosaccharide, 3.5 times purified water, and 0.15 times brown sugar into the fermentation tank, boil for 5 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0075] The fourth step is to prepare the fermentation liquid: the binary yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight respectively, the fermentation temperature is 33° C., and the fermentation time is 36 hours.

[0076] The fifth step is yeast autolysis and ultrasonic cell wall breaking: after the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, the yeast autolysis conditions are controlled at 55°C, 24h, and then the ultrasonic cell wall breaking process is adopted, ultrasonic breaking for 1min, rest for 1min, and breaking 20 times.

[0077] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0078] The seventh step is purification: purification using anion exchange resin TEHUAMP-600.

[0079] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0080] Comparative Example 1

[0081] The first step is to prepare ultrafine powder: take 12 parts of seabuckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plums, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 6 parts of red dates, and 6 parts of licorice. Crush them into powder with a particle size of about 100 μm and set aside.

[0082] The second step is water extraction method: add 10 times the amount of distilled water, soak for 4 hours, heat to boil, decoct for 2 hours, filter, add 5 times the amount of distilled water to the residue, heat to boil, decoct for 1 hour, filter; mix the two filtrates.

[0083] The third step is to obtain the product: after the filtrate is appropriately concentrated, freeze-drying is performed to obtain the product.

[0084] Comparative Example 2

[0085] The first step is to prepare ultrafine powder: take 12 parts of seabuckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plums, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 6 parts of red dates, and 6 parts of licorice. Crush them into powder with a particle size of about 100 μm and set aside.

[0086] The second step is ethanol extraction method: add 10 times the amount of 95% ethanol, soak for 4 hours, heat under reflux for extraction for 2 hours and filter, add 5 times the amount of 95% ethanol to the residue, heat under reflux for extraction for 2 hours and filter; mix the two filtrates.

[0087] The third step is to obtain the product: after the filtrate is appropriately concentrated, freeze-drying is performed to obtain the product.

[0088] Comparative Example 3

[0089] The first step is to prepare ultrafine powder: take 12 parts of seabuckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plums, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 6 parts of red dates, and 6 parts of licorice. Crush them into powder with a particle size of about 100 μm and set aside.

[0090] The second step is to extract with ethanol first and then with water: add 10 times the amount of 95% ethanol, soak for 4 hours, heat and reflux to extract for 2 hours, filter, add 5 times the amount of 95% ethanol to the residue, heat and reflux to extract for 2 hours, filter; mix the two filtrates. Add 10 times the amount of distilled water to the residue, soak for 4 hours, heat and boil for 2 hours, filter, add 5 times the amount of distilled water to the residue, heat and boil for 1 hour, filter; mix the two filtrates with the alcohol extraction filtrate.

[0091] The third step is to obtain the product: after the filtrate is appropriately concentrated, freeze-drying is performed to obtain the product.

[0092] Comparative Example 4

[0093] The first step is to prepare the stock solution: take 12 parts of sea buckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plum, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 2 parts of red dates, 2 parts of lactitol, 2 parts of isomaltooligosaccharide, and 6 parts of licorice. Wash sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, red dates, and licorice separately, soak until there is no dry core, drain the surface water, and crush into stock solution for standby use.

[0094] The second step is to prepare the fermentation agent: the culture medium is 40.5g / L glucose, 36.8g / L peptone, 17.3g / L corn steep liquor, 14g / L potassium nitrate, 1.5g / L yeast nutrient salt, 0.6g / L potassium dihydrogen phosphate, and 0.8g / L magnesium sulfate. Select activated high-temperature resistant and highly active yeast, with an inoculation amount of 2%, 31°C, 160 rpm, and aeration culture for 24 hours to make fermentation liquid A for standby use; select activated Lactobacillus acidophilus, with an inoculation amount of 2%, and culture at 35°C in a closed environment for 48 hours to make binary fermentation liquid B.

[0095] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add lactitol, isomaltooligosaccharide, 3.5 times purified water, and 0.15 times brown sugar into the fermentation tank, boil for 5 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0096] The fourth step is to prepare the fermentation liquid: the binary yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight respectively, the fermentation temperature is 33° C., and the fermentation time is 36 hours.

[0097] The fifth step is yeast autolysis and ultrasonic cell wall breaking: after the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, the yeast autolysis conditions are controlled at 55°C, 24h, and then the ultrasonic cell wall breaking process is adopted, ultrasonic breaking for 1min, rest for 1min, and breaking 20 times.

[0098] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0099] The seventh step is purification: purification using anion exchange resin TEHUAMP-600.

[0100] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0101] Comparative Example 5

[0102] The first step is to prepare the stock solution: take 12 parts of sea buckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plum, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 2 parts of red dates, 2 parts of lactitol, 2 parts of isomaltooligosaccharide, and 6 parts of licorice. Wash sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, red dates, and licorice separately, soak until there is no dry core, drain the surface water, and crush into stock solution for standby use.

[0103] The second step is to prepare the yeast agent: the culture medium is 2% yeast extract powder, 50% water, 9% brown sugar and 39% potato, and activated high-temperature resistant and highly active yeast is selected, the inoculation amount is 2%, 31°C, 160 rpm, and aeration culture is carried out for 24 hours to prepare yeast solution A for standby use; the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% potato, and activated Lactobacillus acidophilus is selected, the inoculation amount is 2%, and closed culture is carried out at 35°C for 48 hours to prepare binary yeast solution B for standby use.

[0104] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add lactitol, isomaltooligosaccharide, 3.5 times purified water, and 0.15 times brown sugar into the fermentation tank, boil for 5 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0105] The fourth step is to prepare the fermentation liquid: the binary yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight respectively, the fermentation temperature is 33° C., and the fermentation time is 36 hours.

[0106] The fifth step is yeast autolysis and ultrasonic cell wall breaking: after the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, the yeast autolysis conditions are controlled at 55°C, 24h, and then the ultrasonic cell wall breaking process is adopted, ultrasonic breaking for 1min, rest for 1min, and breaking 20 times.

[0107] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0108] The seventh step is purification: purification using anion exchange resin TEHUAMP-600.

[0109] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0110] Comparative Example 6

[0111] The first step is to prepare the stock solution: take 12 parts of sea buckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plum, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 2 parts of red dates, 2 parts of lactitol, 2 parts of isomaltooligosaccharide, and 6 parts of licorice. Wash sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, red dates, and licorice separately, soak until there is no dry core, drain the surface water, and crush into stock solution for standby use.

[0112] The second step is to prepare the yeast agent: the culture medium is 2% yeast extract powder, 50% water, 9% brown sugar and 39% potato, and activated Aspergillus oryzae is selected, the inoculation amount is 2%, 31°C, 160 rpm, and aeration culture is carried out for 24 hours to prepare yeast solution A for standby use; the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% potato, and activated Streptococcus thermophilus is selected, the inoculation amount is 2%, and closed culture is carried out at 35°C for 48 hours to prepare binary yeast solution B for standby use.

[0113] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add lactitol, isomaltooligosaccharide, 3.5 times purified water, and 0.15 times brown sugar into the fermentation tank, boil for 5 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0114] The fourth step is to prepare the fermentation liquid: the binary yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight respectively, the fermentation temperature is 33° C., and the fermentation time is 36 hours.

[0115] The fifth step is yeast autolysis and ultrasonic cell wall breaking: after the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, the yeast autolysis conditions are controlled at 55°C, 24h, and then the ultrasonic cell wall breaking process is adopted, ultrasonic breaking for 1min, rest for 1min, and breaking 20 times.

[0116] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0117] The seventh step is purification: purification using anion exchange resin TEHUAMP-600.

[0118] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0119] Comparative Example 7

[0120] The first step is to prepare the stock solution: take 12 parts of sea buckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plum, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 2 parts of red dates, 2 parts of lactitol, 2 parts of isomaltooligosaccharide, and 6 parts of licorice. Wash sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, red dates, and licorice separately, soak until there is no dry core, drain the surface water, and crush into stock solution for standby use.

[0121] The second step is to prepare the yeast agent: the culture medium is 2% yeast extract powder, 50% water, 9% brown sugar and 39% potato, and activated high-temperature resistant and highly active yeast is selected, the inoculation amount is 2%, 31°C, 160 rpm, and aeration culture is carried out for 24 hours to prepare yeast solution A for standby use; the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% potato, and activated Lactobacillus acidophilus is selected, the inoculation amount is 2%, and closed culture is carried out at 35°C for 48 hours to prepare binary yeast solution B for standby use.

[0122] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add lactitol, isomaltooligosaccharide, 3.5 times purified water, and 0.15 times glucose into the fermentation tank, boil for 5 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0123] The fourth step is to prepare the fermentation liquid: the binary yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight respectively, the fermentation temperature is 33° C., and the fermentation time is 36 hours.

[0124] The fifth step is yeast autolysis and ultrasonic cell wall breaking: after the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, the yeast autolysis conditions are controlled at 55°C, 24h, and then the ultrasonic cell wall breaking process is adopted, ultrasonic breaking for 1min, rest for 1min, and breaking 20 times.

[0125] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0126] The seventh step is purification: purification using anion exchange resin TEHUAMP-600.

[0127] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0128] Comparative Example 8

[0129] The first step is to prepare the stock solution: take 12 parts of sea buckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plum, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 2 parts of red dates, 2 parts of lactitol, 2 parts of isomaltooligosaccharide, and 6 parts of licorice. Wash sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, red dates, and licorice separately, soak until there is no dry core, drain the surface water, and crush into stock solution for standby use.

[0130] The second step is to prepare the yeast agent: the culture medium is 2% yeast extract powder, 50% water, 9% brown sugar and 39% potato, and activated high-temperature resistant and highly active yeast is selected, the inoculation amount is 2%, 31°C, 160 rpm, and aeration culture is carried out for 24 hours to prepare yeast solution A for standby use; the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% potato, and activated Lactobacillus acidophilus is selected, the inoculation amount is 2%, and closed culture is carried out at 35°C for 48 hours to prepare binary yeast solution B for standby use.

[0131] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add lactitol, isomaltooligosaccharide, 3.5 times purified water, and 0.15 times brown sugar into the fermentation tank, boil for 5 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0132] The fourth step is to prepare the fermentation liquid: the binary yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight respectively, the fermentation temperature is 33° C., and the fermentation time is 36 hours.

[0133] The fifth step is ultrasonic wall breaking: ultrasonic wall breaking technology is used, ultrasonic breaking for 1 minute, rest for 1 minute, and breaking 20 times.

[0134] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0135] The seventh step is purification: purification using anion exchange resin TEHUAMP-600.

[0136] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0137] Comparative Example 9

[0138] The first step is to prepare the stock solution: take 12 parts of sea buckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plum, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 2 parts of red dates, 2 parts of lactitol, 2 parts of isomaltooligosaccharide, and 6 parts of licorice. Wash sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, red dates, and licorice separately, soak until there is no dry core, drain the surface water, and crush into stock solution for standby use.

[0139] The second step is to prepare the yeast agent: the culture medium is 2% yeast extract powder, 50% water, 9% brown sugar and 39% potato, and activated high-temperature resistant and highly active yeast is selected, the inoculation amount is 2%, 31°C, 160 rpm, and aeration culture is carried out for 24 hours to prepare yeast solution A for standby use; the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% potato, and activated Lactobacillus acidophilus is selected, the inoculation amount is 2%, and closed culture is carried out at 35°C for 48 hours to prepare binary yeast solution B for standby use.

[0140] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add lactitol, isomaltooligosaccharide, 3.5 times purified water, and 0.15 times brown sugar into the fermentation tank, boil for 5 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0141] The fourth step is to prepare the fermentation liquid: the binary yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight respectively, the fermentation temperature is 33° C., and the fermentation time is 36 hours.

[0142] Step 5: yeast autolysis: After the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, and the yeast autolysis conditions are controlled at 55°C and 24h.

[0143] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0144] The seventh step is purification: purification using anion exchange resin TEHUAMP-600.

[0145] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0146] Comparative Example 10

[0147] The first step is to prepare the stock solution: take 12 parts of sea buckthorn, 8 parts of patchouli, 8 parts of perilla, 6 parts of oats, 6 parts of black plum, 6 parts of houttuynia, 6 parts of mint, 6 parts of angelica, 6 parts of Euryale, 2 parts of red dates, 2 parts of lactitol, 2 parts of isomaltooligosaccharide, and 6 parts of licorice. Wash sea buckthorn, patchouli, perilla, oats, black plum, houttuynia, mint, angelica, Euryale, red dates, and licorice separately, soak until there is no dry core, drain the surface water, and crush into stock solution for standby use.

[0148] The second step is ultrafiltration: After the original slurry is fully stirred with 3 times the volume of water, the supernatant is coarsely filtered and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0149] The third step is purification: purification using anion exchange resin TEHUAMP-600.

[0150] The fourth step is drying: freeze-drying the purified product to obtain active polypeptides.

[0151] Comparative Example 11

[0152] The first step is to prepare the stock solution: take 8 parts of ginseng, 6 parts of wolfberry, 6 parts of hawthorn, 6 parts of chicken gizzard lining, 6 parts of orange peel, 8 parts of peach kernel, and 6 parts of chicory. Wash them separately, soak them until there is no dry core, drain the surface water, crush them into stock solution, and set aside.

[0153] The second step is to prepare the fermentation agent: the culture medium is 2% yeast extract powder, 2% glucose and 5% soybean meal, and the balance is water. The activated Paecilomyces punctatus is inoculated at an inoculation rate of 2%, and aerobically cultured at 160 rpm and 25°C for 30 hours to prepare a binary fermentation liquid A; the activated Bifidobacterium lactis is inoculated at an inoculation rate of 2%, and sealed cultured at 36°C for 12 hours to prepare a fermentation liquid B for standby use.

[0154] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add 3.5 times of pure water and 0.15 times of brown sugar into the fermentation tank, boil for 5 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0155] Step 4: prepare the fermentation liquid: inoculate the fermentation liquid A and B in the second step into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight, respectively, and ferment at a closed temperature of 33° C. for 36 hours.

[0156] The fifth step is ultrasonic cell wall breaking: after the fermentation is terminated, ultrasonic cell wall breaking technology is used, ultrasonic breaking for 1 minute, rest for 1 minute, and breaking 20 times.

[0157] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 6000 Daltons to remove macromolecular impurities and obtain components such as polypeptides below 6000 Daltons.

[0158] Step 7: Purification: Purification using 724 cation exchange resin.

[0159] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0160] Comparative Example 12

[0161] The first step is to prepare the stock solution: take 8 parts of ginseng, 6 parts of lotus seeds, 6 parts of Amomum villosum, 8 parts of Chinese yam, 6 parts of Poria cocos, 8 parts of white hyacinth bean, 6 parts of orange peel, 6 parts of licorice, and 6 parts of jujube. Wash them separately, soak them until there is no dry core, drain the surface water, crush them into stock solution, and set aside.

[0162] The second step is to prepare the fermentation agent: the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% soybean meal superfine powder. Select activated Kluyveromyces marxii, inoculated at 2.5%, 160 rpm, 28℃ sealed fermentation for 8 hours, and then aerate and culture for 30 hours to make fermentation liquid A; select activated Lactobacillus bulgaricus, Bifidobacterium longum, and Lactobacillus acidophilus inoculated at a ratio of 1:1:1, inoculated at 2% respectively, and mixed cultured at 36℃ for 12 hours to make fermentation liquid B for standby use.

[0163] The third step is to prepare the diluted mixed slurry: according to the weight of the original slurry obtained in the first step, add 3.5 times of pure water and 0.15 times of brown sugar into the fermentation tank, boil for 5 minutes, stop heating, cool down, and obtain the diluted mixed slurry for standby use.

[0164] The fourth step is to prepare the fermentation liquid: the yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight, respectively. The fermentation temperature is 33° C. and the time is 36 hours.

[0165] The fifth step is yeast autolysis and ultrasonic cell wall breaking: after the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, the yeast autolysis conditions are controlled at 55°C, 24h, and then the ultrasonic cell wall breaking process is adopted, ultrasonic breaking for 1min, rest for 1min, and breaking 20 times.

[0166] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0167] Step 7: Purification: Purification using Tehua IRA204 anion exchange resin.

[0168] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0169] Comparative Example 13

[0170] The first step is to prepare the stock solution: take 8 parts of bergamot, 8 parts of amomum, 8 parts of citron, 6 parts of poria, 6 parts of malt, 6 parts of cordyceps militaris, 6 parts of jujube, 6 parts of licorice, 6 parts of ginseng, 3 parts of sorbitol, 3 parts of arabinose, 2 parts of tea theanine, and 6 parts of mint. Wash bergamot, amomum, citron, poria, malt, cordyceps militaris, jujube, licorice, ginseng, and mint separately, soak until there is no dry core, drain the surface water, crush into stock solution, and set aside.

[0171] The second step is to prepare the fermentation agent: the culture medium is 1% yeast extract powder, 77% water, 4% brown sugar and 18% potato. Select activated high temperature resistant high activity yeast and Aspergillus oryzae in the inoculation ratio of 1:1, the inoculation amount is 2.5%, 160 rpm, 34℃ mixed culture for 30h, to make binary fermentation liquid A; select activated Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus in the inoculation ratio of 1:1:1, the inoculation amount is 2%, 36℃ mixed culture for 12h, to make fermentation liquid B, and set aside.

[0172] The third step is to prepare the diluted mixed slurry: based on the weight of the original slurry obtained in the first step, add sorbitol, arabinose, tea theanine, 3.5 times of pure water, and 0.15 times of brown sugar to the original slurry in the fermentation tank, boil it for 5 minutes, stop heating, cool it down, and obtain the diluted mixed slurry for standby use.

[0173] The fourth step is to prepare the fermentation liquid: the binary yeast liquid A and the yeast liquid B in the second step are inoculated into the diluted mixed slurry obtained in the third step at a ratio of 0.04 times by weight respectively, the fermentation temperature is 33° C., and the fermentation time is 36 hours.

[0174] The fifth step is yeast autolysis and ultrasonic cell wall breaking: after the fermentation is terminated, the yeast in the fermentation liquid is autolyzed, the yeast autolysis conditions are controlled at 55°C, 24h, and then the ultrasonic cell wall breaking process is adopted, ultrasonic breaking for 1min, rest for 1min, and breaking 20 times.

[0175] The sixth step is ultrafiltration: the supernatant is taken and separated using an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons to remove large molecular impurities and obtain small molecular peptides and other components below 10,000 Daltons.

[0176] Step 7: Purification: Purification using 732 type cation exchange resin.

[0177] Step 8: Drying: The purified product is freeze-dried to obtain the active polypeptide.

[0178] Experimental Example 1

[0179] The results are shown in Table 1:

[0180]

[0181]

[0182] As can be seen from Table 1, the polypeptide yield, polypeptide purity and histamine content of the corresponding products obtained from the raw materials of seabuckthorn, patchouli, perilla, oats, ebony, houttuynia, mint, angelica dahurica, Euryale ferox, red dates, lactitol, isomaltooligosaccharide, licorice and the like in Examples 1 to 5 of the present invention are significantly improved compared with the relevant comparative examples.

[0183] Experimental Example 2

[0184] 1. Methods

[0185] Rats were randomly divided into a normal group, a model group, groups 1 to 5 of embodiments and each group of the comparative example, with 10 rats in each group. The model group, groups 1 to 5 of embodiments and each group of the comparative example were given 1 mg / mL ovalbumin (OVA) by gavage (1 mL / d), and no immune adjuvant was used throughout the process; the normal group was given normal saline by gavage (1 mL / d); at the same time, the daily gavage dosage of groups 1 to 5 of embodiments and each group of the comparative example was calculated according to the standard animal equivalent dose conversion coefficient method (based on 50g of raw medicinal materials / kg body weight / day for rats), and the rats in the normal group and the model group were gavaged with starch of equal weight, twice a day. After continuous gavage for 30 days, the model group, groups 1 to 5 of embodiments and each group of the comparative example were given OVA 100 mg / rat gavage on the 31st day. After 1 hour, about 1 mL of blood was taken from the medial canthus vein of each group of rats, and the serum was separated and placed in a -20°C refrigerator for testing. During this period, the rats were free to eat and drink water.

[0186] The blood samples of rats were collected by ophthalmic venous plexus blood collection method into tube A (anticoagulant tube containing heparin, used for separating plasma) and tube B (without anticoagulant, used for separating serum). After collection in tube A, the samples were mixed and centrifuged at 3000 rpm for 15 min to obtain plasma, which was then aliquoted and placed in a -20℃ refrigerator for later use; tube B was placed in a 37℃ water bath for 1 hour, then centrifuged at 3000 rpm for 15 min, the supernatant was aspirated and aliquoted, and frozen at -20℃ for later use. Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum OVA-IgE antibody and plasma histamine levels of the two groups of rats. The OVA-IgE ELISA and histamine ELISA kits were from TaKaRa.

[0187] 2. Results

[0188] Statistical results of serum OVA-IgE antibody and plasma histamine determination in rats of each group See Table 2.

[0189] Table 2

[0190]

[0191]

[0192] The links not described in detail in the present invention are all common knowledge that can be selected by ordinary technicians in the field. Although the present invention has been described in detail above with general descriptions and specific implementation schemes, it is obvious to ordinary technicians in the field that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. A composite peptide dietary supplement for improving special constitution, characterized in that: The composite peptide dietary supplement comprises the following raw materials: seabuckthorn, patchouli, perilla, oat, black plum, houttuynia cordata, mint, angelica dahurica, euryale ferox, red dates, lactitol and isomaltooligosaccharide.

2. The composite peptide dietary supplement according to claim 1, characterized in that: The composite peptide dietary supplement raw material also includes liquorice.

3. The composite peptide dietary supplement according to claim 2, characterized in that: In terms of weight, the sea buckthorn is 9-15 parts, the patchouli is 2-10 parts, the perilla is 2-10 parts, the oats are 2-8 parts, the black plums are 2-8 parts, the houttuynia cordata is 2-8 parts, the mint is 2-8 parts, the angelica dahurica is 2-8 parts, the Euryale ferox is 2-8 parts, the red dates are 2-8 parts, the lactitol is 1-3 parts, the isomaltooligosaccharide is 1-3 parts, and the licorice is 2-8 parts.

4. The composite peptide dietary supplement according to claim 2, characterized in that: In terms of weight, the seabuckthorn is 12 parts, the patchouli is 8 parts, the perilla is 8 parts, the oats is 6 parts, the black plum is 6 parts, the houttuynia cordata is 6 parts, the mint is 6 parts, the angelica is 6 parts, the euryale is 6 parts, the red dates are 6 parts, the lactitol is 2 parts, the isomaltooligosaccharide is 2 parts, and the licorice is 6 parts; or the seabuckthorn is 10 parts, the patchouli is 8 parts, the perilla is 8 parts, the oats is 6 parts, the black plum is 6 parts, the houttuynia cordata is 6 parts, the mint is 6 parts, the angelica is 4 parts, the euryale is 4 parts, the red dates are 2 parts, the lactitol is 2 parts, the isomaltooligosaccharide is 2 parts, and the licorice is 4 parts.

5. The method for preparing the composite peptide dietary supplement according to any one of claims 1 to 4, characterized in that: The following steps are involved: The first step is to prepare the original slurry; the second step is to prepare the yeast agent; the third step is to prepare the diluted mixed slurry; the fourth step is to prepare the fermentation liquid; the fifth step is to autolyze the yeast and break the wall with ultrasonic wave; the sixth step is to ultrafilter; the seventh step is to purify; and the eighth step is to dry.

6. The method for preparing the composite peptide dietary supplement according to claim 5, characterized in that: The specific process of preparing the stock solution in the first step is to take sea buckthorn, patchouli, perilla, oat, black plum, houttuynia, mint, angelica dahurica, Euryale ferox, red dates, licorice and other raw materials, wash them separately, soak them until there is no dry core, drain the surface water, crush them into stock solution, and set aside; The specific process of preparing the fermentation agent in the second step is to select high-temperature resistant and highly active yeast for ventilation culture to form a binary fermentation liquid A, and to culture Lactobacillus acidophilus in a closed manner to form a fermentation liquid B for standby use; The specific process of preparing the diluted mixed slurry in the third step is to add lactitol, isomaltooligosaccharide, an appropriate amount of purified water, and an appropriate amount of brown sugar into the fermentation tank according to the weight of the original slurry obtained in the first step, keep boiling for a period of time, stop heating, cool down, and obtain the diluted mixed slurry for standby use; The specific process of preparing the fermentation liquid in the fourth step is to inoculate the binary yeast liquid A and yeast liquid B in the second step into the diluted mixed slurry obtained in the third step at a ratio of 0.02 to 0.06 times by weight; The specific process of the fifth step of yeast autolysis and ultrasonic cell wall breaking is to control appropriate yeast autolysis conditions after terminating the fermentation, autolyze the yeast in the fermentation broth and use ultrasonic cell wall breaking extraction process to obtain rich yeast small molecule peptides and amino acids; The specific process of the sixth step of ultrafiltration is to take the supernatant and use an ultrafiltration membrane with a molecular weight cutoff of 10,000 Daltons for membrane separation to remove macromolecular impurities and obtain small molecular peptides and other components below 10,000 Daltons; The specific process of the seventh step of purification is purification using anion exchange resin TEHUAMP-600; The specific process of the eighth step of drying is to freeze-dry the purified product to obtain the active polypeptide or spray-dry it.

7. The method for preparing the composite peptide dietary supplement according to claim 6, characterized in that: In the second step, the high-activity, high-temperature-resistant and high-activity yeast is inoculated into a culture medium at a temperature of 29 to 33° C., cultured at 160 rpm for 12 to 36 hours to prepare a binary fermentation liquid A; Lactobacillus acidophilus is inoculated into a culture medium at a temperature of 33 to 37° C., cultured for 36 to 60 hours to prepare a fermentation liquid B; in the third step, based on the weight of the original slurry obtained in the first step, lactitol, isomaltooligosaccharide, 2 to 5 times of pure water, and 0.1 to 0.2 times of brown sugar are added to the original slurry in the fermentation tank, the mixture is boiled and kept for 5 minutes, the heating is stopped, the temperature is lowered, and a diluted mixed slurry is obtained for standby use; in the fourth step, the fermentation time is 1 to 2 days.

8. The method for preparing the composite peptide dietary supplement according to claim 6, characterized in that: In the second step, the culture medium of the yeast agent is yeast extract powder, brown sugar and potato.

9. A preparation of a composite peptide dietary supplement for improving a special constitution, characterized in that: The preparation is in the form of tablets, granules or oral liquid.

10. Use of the composite peptide dietary supplement according to claims 1 to 5, characterized in that: The application refers to the use in preparing food or medicine suitable for people with special constitutions.

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