Process for extracting peony flower cell water from peony flowers

Abstract

The invention provides a process for extracting fresh peony flower cell water from fresh peony flowers. The extraction process comprises the following steps: A, firstly, picking fresh peony flowers; b, the picked peony flowers are pretreated; c, performing shearing treatment on the pretreated peony flowers; d, the sheared peony flowers are added into a presser to be pressed, and pressed residues and filtrate are obtained; e, adding the pressure slag into a distillation retort for primary distillation treatment, and filtering to obtain primary distillate; f, mixing the filtrate and the primary distillate, adding the mixture into a reaction kettle, and adding enzymatic hydrolysate for enzymolysis treatment to obtain a primary enzymolysis solution; and G, filtering the primary enzymolysis solution, adding the filtered solution into a distillation retort, carrying out secondary distillation treatment, and filtering to obtain a secondary distillate. The extracted cell water does not contain impurity ions and harmful substances, spice components and thermosensitive substances in the peony petals are reserved, and the activity of cosmetics can be effectively improved when the cell water is added into the cosmetics.

Description

Technical Field

[0001] The present application relates to the technical field of cell water extraction, and in particular to a process for extracting peony flower cell water from peony flowers. Background Art

[0002] Peony flowers are brightly colored and elegant. There are hundreds of varieties classified according to different colors. The flowers are large and fragrant, and are known as "national beauty and heavenly fragrance". Peony flowers have been hailed as the king of flowers since ancient times, and there are rich cultural and painting works. Peony flowers are perennial deciduous shrubs that grow slowly and have a small plant shape. The plant height is mostly between 0.5 and 2 meters. The roots are fleshy, thick and long, with a woody center, and are generally 0.5 to 0.8 meters long. A few roots can reach 2 meters in length. The color of the root bark and root flesh varies with the variety. The branches of peonies are upright and brittle, round, and are clustered from the rhizome to form a shrub-like shape. The branches of the current year are smooth, herbaceous, yellow-brown, and often cracked and peeled off. The leaves are alternate, and the leaves are usually two-three compound leaves. The upper part of the peony branch is often single-leaved, with leaflets in shapes such as lanceolate, oval, and elliptical. The terminal leaflets are often 2 to 3-lobed, dark green or yellow-green on the upper side, gray-green on the lower side, smooth or hairy; the peony petiole is 8 to 20 cm long, with grooves on the surface; the flowers are solitary at the top of the branches of the year, bisexual, large and colorful, beautiful in shape, with a diameter of 10 to 30 cm; the colors of the flowers include white, yellow, pink, red, purple-red, purple, ink purple (black), snow blue (pink blue), green, and complex colors.

[0003] At present, the process of extracting cellular water from peony flowers is complicated. A large amount of organic solvents are added during the extraction process, which affects the activity cost of the cellular water and easily introduces harmful substances. Therefore, it is necessary to design a process for extracting peony flower cellular water from peony flowers. Summary of the invention

[0004] The present application provides a process for extracting peony flower cell water from peony flowers, so as to solve the problems in the prior art of complicated extraction steps and impurities in the extracted cell water, thereby achieving the goal of containing no impurity ions and harmful substances in the extracted cell water and retaining the fragrance components, heat-sensitive substances and other active ingredients in the peony petals.

[0005] The present application provides a process for extracting peony flower cell water from peony flowers, and the extraction process comprises the following steps: A. First, pick fresh peony flowers; B, pre-treating the picked peony flowers; C, shearing the pretreated peony flowers; D. Add the sheared peony flowers into a squeezer, add deionized water for squeezing, and filter to obtain a pressed residue and a filtrate; E. adding the pressed slag into a distillation tank for primary distillation, filtering, and obtaining a primary distillate; F. Mixing the filtrate and the primary distillate, adding the mixture into a reactor, and adding an enzymolysis solution for enzymolysis to obtain a primary enzymolysis solution; G. After filtering the primary enzymatic solution, the filtered solution is added to a distillation tank for secondary distillation, filtered, and a secondary distillate is obtained; H. adding enzymolysis solution to the secondary distilled liquid for enzymolysis treatment to obtain a secondary enzymolysis solution; 1. Adding the secondary enzymolysis solution into a centrifuge and performing a centrifugation to obtain a centrifuge; J. Adding the enzymatic hydrolysis solution into the centrifugal solution again for enzymatic hydrolysis, and then performing a second centrifugation, and filtering to obtain peony flower cell water.

[0006] Preferably, the pretreatment method in step B is as follows: a. Rinse the peony flowers in clean water for 3-5 times and dry them at room temperature; b. Then immerse the cleaned peony flowers in the ethanol solution for 10-18 minutes, take them out, rinse them with clean water again, and dry them at room temperature.

[0007] Preferably, in step C, the processed peony flowers are added to a shearing machine and sheared into fragments with a side length of 10 mm-15 mm.

[0008] Preferably, in step D, the peony flower is squeezed and ultrasonicated simultaneously, the ultrasonic frequency is 20-30 kHz, and the time is 30 min-40 min.

[0009] Preferably, the first distillation temperature in step E is 75°C-90°C, and the time is 20min-30min; the second distillation temperature in step G is 85°C-95°C, and the time is 10min-20min.

[0010] Preferably, the enzymatic hydrolysate components include 2-5 parts of α-amylase, 1-4 parts of β-amylase, 1-4 parts of pullulanase, 2-6 parts of hemicellulase, and 2-6 parts of alkaline protease by weight.

[0011] Preferably, in step I, the centrifugation rate is 2000-4000 rpm, and the centrifugation time is 15 min-25 min.

[0012] Preferably, in step J, the secondary centrifugation rate is 3000-5000 rpm and the time is 20 min-30 min. Beneficial Effects

[0013] (1) The extraction process of the present invention is simple. The peony used in the present invention is selected from Heyang, Shaanxi and Luoyang, Henan. The cell water extracted by the extraction process of the present invention does not contain impurity ions and harmful substances, and retains the fragrance components, heat-sensitive substances and other active ingredients in the peony petals. The cell water is added to cosmetics to effectively improve the activity of the cosmetics.

[0014] (2) In the extraction process of the present invention, the steps of performing a first distillation, a first enzymatic hydrolysis, a second distillation, a second enzymatic hydrolysis, a first centrifugation, a second enzymatic hydrolysis, and a second centrifugation on the peony flower can improve the content and purity of cell water.

[0015] (3) The enzymatic hydrolysate used in the present invention can release the active substances in the peony flowers to the greatest extent, further increasing the content of effective ingredients in the cell water.

[0016] The above description is only an overview of the technical solution of the embodiment of the present application. In order to more clearly understand the technical means of the embodiment of the present application, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the embodiment of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field to which this application belongs; the terms used in the specification of the application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0019] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase "embodiments" in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0020] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application is clearly and completely described below. Embodiment 1;

[0021] The present application provides a process for extracting peony flower cell water from peony flowers, and the extraction process comprises the following steps: A. First, pick fresh peony flowers; B, pre-treating the picked peony flowers; C, shearing the pretreated peony flowers; D. Add the sheared peony flowers into a squeezer, add deionized water for squeezing, and filter to obtain a pressed residue and a filtrate; E. adding the pressed slag into a distillation tank for primary distillation, filtering, and obtaining a primary distillate; F. Mixing the filtrate and the primary distillate, adding the mixture into a reactor, and adding an enzymolysis solution for enzymolysis to obtain a primary enzymolysis solution; G. After filtering the primary enzymatic solution, the filtered solution is added to a distillation tank for secondary distillation, filtered, and a secondary distillate is obtained; H. adding enzymolysis solution to the secondary distilled liquid for enzymolysis treatment to obtain a secondary enzymolysis solution; 1. Adding the secondary enzymolysis solution into a centrifuge and performing a centrifugation to obtain a centrifuge; J. Adding the enzymatic hydrolysis solution into the centrifugal solution again for enzymatic hydrolysis, and then performing a second centrifugation, and filtering to obtain peony flower cell water.

[0022] In this embodiment, the pretreatment method in step B is as follows: a. Rinse the peony flowers in clean water for 3 times and dry them at room temperature; b. Then immerse the cleaned peony flowers in the ethanol solution for 10 minutes, take them out, rinse them with clean water again, and dry them at room temperature.

[0023] In this embodiment, in step C, the processed peony flowers are added to a shearing machine and cut into pieces with a side length of 10 mm.

[0024] In this embodiment, in step D, ultrasonication is performed while squeezing the peony flowers, and the ultrasonic frequency is 20 kHz and the time is 30 minutes.

[0025] In this embodiment, the first distillation temperature in step E is 75° C. and the time is 20 min; the second distillation temperature in step G is 85° C. and the time is 10 min.

[0026] In this embodiment, the enzymatic hydrolysate components include 2 parts of α-amylase, 1 part of β-amylase, 1 part of pullulanase, 2 parts of hemicellulase, and 2 parts of alkaline protease by weight. The enzymatic hydrolysate used in the present invention can release the active substances in the peony flower to the greatest extent, and further increase the content of effective ingredients in the cell water.

[0027] In this embodiment, the centrifugal speed in step I is 2000 rpm and the centrifugal time is 15 min.

[0028] In this embodiment, the secondary centrifugation rate in step J is 3000 rpm and the time is 20 min. Embodiment 2:

[0029] A process for extracting peony flower cell water from peony flowers, the extraction process comprising the following steps: A. First, pick fresh peony flowers; B, pre-treating the picked peony flowers; C, shearing the pretreated peony flowers; D. Add the sheared peony flowers into a squeezer, add deionized water for squeezing, and filter to obtain a pressed residue and a filtrate; E. adding the pressed slag into a distillation tank for primary distillation, filtering, and obtaining a primary distillate; F. Mixing the filtrate and the primary distillate, adding the mixture into a reactor, and adding an enzymolysis solution for enzymolysis to obtain a primary enzymolysis solution; G. After filtering the primary enzymatic solution, the filtered solution is added to a distillation tank for secondary distillation, filtered, and a secondary distillate is obtained; H. adding enzymolysis solution to the secondary distilled liquid for enzymolysis treatment to obtain a secondary enzymolysis solution; 1. Adding the secondary enzymolysis solution into a centrifuge and performing a centrifugation to obtain a centrifuge; J. Adding the enzymatic hydrolysis solution into the centrifugal solution again for enzymatic hydrolysis, and then performing a second centrifugation, and filtering to obtain peony flower cell water.

[0030] In this embodiment, the pretreatment method in step B is as follows: a. Rinse the peony flowers in clean water for 5 times and dry them at room temperature; b. Then immerse the cleaned peony flowers in the ethanol solution for 18 minutes, take them out, rinse them with clean water again, and dry them at room temperature.

[0031] In this embodiment, in step C, the processed peony flowers are added to a shearing machine and sheared into pieces with a side length of 15 mm.

[0032] In this embodiment, in step D, ultrasonication is performed while squeezing the peony flowers, and the ultrasonic frequency is 30 kHz and the time is 40 minutes.

[0033] In this embodiment, the first distillation temperature in step E is 90° C. and the time is 30 min; the second distillation temperature in step G is 95° C. and the time is 20 min.

[0034] In this embodiment, the enzymatic hydrolysate components include 5 parts of α-amylase, 4 parts of β-amylase, 4 parts of pullulanase, 6 parts of hemicellulase, and 6 parts of alkaline protease by weight. The enzymatic hydrolysate used in the present invention can release the active substances in the peony flower to the maximum extent, and further increase the content of effective ingredients in cell water.

[0035] In this embodiment, the centrifugal speed in step I is 4000 rpm and the centrifugal time is 25 min.

[0036] In this embodiment, the secondary centrifugation rate in step J is 5000 rpm and the time is 30 min. Embodiment three;

[0037] A process for extracting peony flower cell water from peony flowers, the extraction process comprising the following steps: A. First, pick fresh peony flowers; B, pre-treating the picked peony flowers; C, shearing the pretreated peony flowers; D. Add the sheared peony flowers into a squeezer, add deionized water for squeezing, and filter to obtain a pressed residue and a filtrate; E. adding the pressed slag into a distillation tank for primary distillation, filtering, and obtaining a primary distillate; F. Mixing the filtrate and the primary distillate, adding the mixture into a reactor, and adding an enzymolysis solution for enzymolysis to obtain a primary enzymolysis solution; G. After filtering the primary enzymatic solution, the filtered solution is added to a distillation tank for secondary distillation, filtered, and a secondary distillate is obtained; H. adding enzymolysis solution to the secondary distilled liquid for enzymolysis treatment to obtain a secondary enzymolysis solution; 1. Adding the secondary enzymolysis solution into a centrifuge and performing a centrifugation to obtain a centrifuge; J. Adding the enzymatic hydrolysis solution into the centrifugal solution again for enzymatic hydrolysis, and then performing a second centrifugation, and filtering to obtain peony flower cell water.

[0038] In this embodiment, the pretreatment method in step B is as follows: a. Rinse the peony flowers in clean water for 3 times and dry them at room temperature; b. Then immerse the cleaned peony flowers in the ethanol solution for 11 minutes, take them out, rinse them with clean water again, and dry them at room temperature.

[0039] In this embodiment, in step C, the processed peony flowers are added to a shearing machine and cut into pieces with a side length of 11 mm.

[0040] In this embodiment, in step D, ultrasonication is performed while squeezing the peony flowers, and the ultrasonic frequency is 22 kHz and the time is 32 minutes.

[0041] In this embodiment, the first distillation temperature in step E is 77° C. and the time is 22 min; the second distillation temperature in step G is 87° C. and the time is 12 min.

[0042] In this embodiment, the enzymatic hydrolysate components include 3 parts of α-amylase, 2 parts of β-amylase, 2 parts of pullulanase, 3 parts of hemicellulase, and 3 parts of alkaline protease by weight. The enzymatic hydrolysate used in the present invention can release the active substances in the peony flower to the maximum extent, and further increase the content of effective ingredients in the cell water.

[0043] In this embodiment, the centrifugal speed in step I is 2300 rpm and the centrifugal time is 17 min.

[0044] In this embodiment, the secondary centrifugation rate in step J is 3200 rpm and the time is 22 min. Embodiment 4:

[0045] A process for extracting peony flower cell water from peony flowers, the extraction process comprising the following steps: A. First, pick fresh peony flowers; B, pre-treating the picked peony flowers; C, shearing the pretreated peony flowers; D. Add the sheared peony flowers into a squeezer, add deionized water for squeezing, and filter to obtain a pressed residue and a filtrate; E. adding the pressed slag into a distillation tank for primary distillation, filtering, and obtaining a primary distillate; F. Mixing the filtrate and the primary distillate, adding the mixture into a reactor, and adding an enzymolysis solution for enzymolysis to obtain a primary enzymolysis solution; G. After filtering the primary enzymatic solution, the filtered solution is added to a distillation tank for secondary distillation, filtered, and a secondary distillate is obtained; H. adding enzymolysis solution to the secondary distilled liquid for enzymolysis treatment to obtain a secondary enzymolysis solution; 1. Adding the secondary enzymolysis solution into a centrifuge and performing a centrifugation to obtain a centrifuge; J. Adding the enzymatic hydrolysis solution into the centrifugal solution again for enzymatic hydrolysis, and then performing a second centrifugation, and filtering to obtain peony flower cell water.

[0046] In this embodiment, the pretreatment method in step B is as follows: a. Rinse the peony flowers in clean water for 5 times and dry them at room temperature; b. Then immerse the cleaned peony flowers in the ethanol solution for 17 minutes, take them out, rinse them with clean water again, and dry them at room temperature.

[0047] In this embodiment, in step C, the processed peony flowers are added to a shearing machine and sheared into pieces with a side length of 14 mm.

[0048] In this embodiment, in step D, ultrasonication is performed while squeezing the peony flowers, and the ultrasonic frequency is 28 kHz and the time is 38 minutes.

[0049] In this embodiment, the first distillation temperature in step E is 88° C. and the time is 28 min; the second distillation temperature in step G is 92° C. and the time is 18 min.

[0050] In this embodiment, the enzymatic solution components include 4 parts of α-amylase, 3 parts of β-amylase, 3 parts of pullulanase, 5 parts of hemicellulase, and 5 parts of alkaline protease by weight. The enzymatic solution used in the present invention can release the active substances in the peony flower to the greatest extent, and further increase the content of effective ingredients in the cell water.

[0051] In this embodiment, the centrifugal speed in step I is 3600 rpm and the centrifugal time is 22 min.

[0052] In this embodiment, the secondary centrifugation rate in step J is 4800 rpm and the time is 28 min. Embodiment five;

[0053] A process for extracting peony flower cell water from peony flowers, the extraction process comprising the following steps: A. First, pick fresh peony flowers; B, pre-treating the picked peony flowers; C, shearing the pretreated peony flowers; D. Add the sheared peony flowers into a squeezer, add deionized water for squeezing, and filter to obtain a pressed residue and a filtrate; E. adding the pressed slag into a distillation tank for primary distillation, filtering, and obtaining a primary distillate; F. Mixing the filtrate and the primary distillate, adding the mixture into a reactor, and adding an enzymolysis solution for enzymolysis to obtain a primary enzymolysis solution; G. After filtering the primary enzymatic solution, the filtered solution is added to a distillation tank for secondary distillation, filtered, and a secondary distillate is obtained; H. adding enzymolysis solution to the secondary distilled liquid for enzymolysis treatment to obtain a secondary enzymolysis solution; 1. Adding the secondary enzymolysis solution into a centrifuge and performing a centrifugation to obtain a centrifuge; J. Adding the enzymatic hydrolysis solution into the centrifugal solution again for enzymatic hydrolysis, and then performing a second centrifugation, and filtering to obtain peony flower cell water.

[0054] In this embodiment, the pretreatment method in step B is as follows: a. Rinse the peony flowers in clean water for 5 times and dry them at room temperature; b. Then immerse the cleaned peony flowers in the ethanol solution for 13 minutes, take them out, rinse them with clean water again, and dry them at room temperature.

[0055] In this embodiment, in step C, the processed peony flowers are added to a shearing machine and cut into pieces with a side length of 11 mm.

[0056] In this embodiment, in step D, ultrasonication is performed while squeezing the peony flowers, and the ultrasonic frequency is 24 kHz and the time is 33 minutes.

[0057] In this embodiment, the first distillation temperature in step E is 79° C. and the time is 24 min; the second distillation temperature in step G is 88° C. and the time is 14 min.

[0058] In this embodiment, the enzymatic hydrolysate components include 3 parts of α-amylase, 3 parts of β-amylase, 2 parts of pullulanase, 5 parts of hemicellulase, and 3 parts of alkaline protease by weight. The enzymatic hydrolysate used in the present invention can release the active substances in the peony flower to the maximum extent, and further increase the content of effective ingredients in the cell water.

[0059] In this embodiment, the centrifugal speed in step I is 2500 rpm and the centrifugal time is 17 min.

[0060] In this embodiment, the secondary centrifugation rate in step J is 3400 rpm and the time is 24 min. Embodiment six;

[0061] A process for extracting peony flower cell water from peony flowers, the extraction process comprising the following steps: A. First, pick fresh peony flowers; B, pre-treating the picked peony flowers; C, shearing the pretreated peony flowers; D. Add the sheared peony flowers into a squeezer, add deionized water for squeezing, and filter to obtain a pressed residue and a filtrate; E. adding the pressed slag into a distillation tank for primary distillation, filtering, and obtaining a primary distillate; F. Mixing the filtrate and the primary distillate, adding the mixture into a reactor, and adding an enzymolysis solution for enzymolysis to obtain a primary enzymolysis solution; G. After filtering the primary enzymatic solution, the filtered solution is added to a distillation tank for secondary distillation, filtered, and a secondary distillate is obtained; H. adding enzymolysis solution to the secondary distilled liquid for enzymolysis treatment to obtain a secondary enzymolysis solution; 1. Adding the secondary enzymolysis solution into a centrifuge and performing a centrifugation to obtain a centrifuge; J. Adding the enzymatic hydrolysis solution into the centrifugal solution again for enzymatic hydrolysis, and then performing a second centrifugation, and filtering to obtain peony flower cell water.

[0062] In this embodiment, the pretreatment method in step B is as follows: a. Rinse the peony flowers in clean water for 3 times and dry them at room temperature; b. Then immerse the cleaned peony flowers in the ethanol solution for 17 minutes, take them out, rinse them with clean water again, and dry them at room temperature.

[0063] In this embodiment, in step C, the processed peony flowers are added to a shearing machine and sheared into pieces with a side length of 12 mm.

[0064] In this embodiment, in step D, ultrasonication is performed while squeezing the peony flowers, and the ultrasonic frequency is 26 kHz and the time is 36 minutes.

[0065] In this embodiment, the first distillation temperature in step E is 85° C. and the time is 27 min; the second distillation temperature in step G is 92° C. and the time is 16 min.

[0066] In this embodiment, the enzymatic hydrolysate components include 4 parts of α-amylase, 2 parts of β-amylase, 2 parts of pullulanase, 5 parts of hemicellulase, and 5 parts of alkaline protease by weight. The enzymatic hydrolysate used in the present invention can release the active substances in the peony flower to the maximum extent, and further increase the content of effective ingredients in the cell water.

[0067] In this embodiment, the centrifugal speed in step I is 2900 rpm and the centrifugal time is 19 min.

[0068] In this embodiment, the secondary centrifugation rate in step J is 4200 rpm and the time is 27 min. Embodiment seven;

[0069] A process for extracting peony flower cell water from peony flowers, the extraction process comprising the following steps: A. First, pick fresh peony flowers; B, pre-treating the picked peony flowers; C, shearing the pretreated peony flowers; D. Add the sheared peony flowers into a squeezer, add deionized water for squeezing, and filter to obtain a pressed residue and a filtrate; E. adding the pressed slag into a distillation tank for primary distillation, filtering, and obtaining a primary distillate; F. Mixing the filtrate and the primary distillate, adding the mixture into a reactor, and adding an enzymolysis solution for enzymolysis to obtain a primary enzymolysis solution; G. After filtering the primary enzymatic solution, the filtered solution is added to a distillation tank for secondary distillation, filtered, and a secondary distillate is obtained; H. adding enzymolysis solution to the secondary distilled liquid for enzymolysis treatment to obtain a secondary enzymolysis solution; 1. Adding the secondary enzymolysis solution into a centrifuge and performing a centrifugation to obtain a centrifuge; J. Adding the enzymatic hydrolysis solution into the centrifugal solution again for enzymatic hydrolysis, and then performing a second centrifugation, and filtering to obtain peony flower cell water.

[0070] In this embodiment, the pretreatment method in step B is as follows: a. Rinse the peony flowers in clean water for 3 times and dry them at room temperature; b. Then immerse the cleaned peony flowers in the ethanol solution for 18 minutes, take them out, rinse them with clean water again, and dry them at room temperature.

[0071] In this embodiment, in step C, the processed peony flowers are added to a shearing machine and cut into pieces with a side length of 10 mm.

[0072] In this embodiment, in step D, ultrasonication is performed while squeezing the peony flowers, and the ultrasonic frequency is 30 kHz and the time is 30 minutes.

[0073] In this embodiment, the first distillation temperature in step E is 80° C. and the time is 23 min; the second distillation temperature in step G is 90° C. and the time is 17 min.

[0074] In this embodiment, the enzymatic hydrolysate components include 2 parts of α-amylase, 4 parts of β-amylase, 1 part of pullulanase, 6 parts of hemicellulase, and 2 parts of alkaline protease by weight. The enzymatic hydrolysate used in the present invention can release the active substances in the peony flower to the greatest extent, and further increase the content of effective ingredients in the cell water.

[0075] In this embodiment, the centrifugal speed in step I is 4000 rpm and the centrifugal time is 15 min.

[0076] In this embodiment, the secondary centrifugation rate in step J is 5000 rpm and the time is 20 min. Embodiment eight;

[0077] A process for extracting peony flower cell water from peony flowers, the extraction process comprising the following steps: A. First, pick fresh peony flowers; B, pre-treating the picked peony flowers; C, shearing the pretreated peony flowers; D. Add the sheared peony flowers into a squeezer, add deionized water for squeezing, and filter to obtain a pressed residue and a filtrate; E. adding the pressed slag into a distillation tank for primary distillation, filtering, and obtaining a primary distillate; F. Mixing the filtrate and the primary distillate, adding the mixture into a reactor, and adding an enzymolysis solution for enzymolysis to obtain a primary enzymolysis solution; G. After filtering the primary enzymatic solution, the filtered solution is added to a distillation tank for secondary distillation, filtered, and a secondary distillate is obtained; H. adding enzymolysis solution to the secondary distilled liquid for enzymolysis treatment to obtain a secondary enzymolysis solution; 1. Adding the secondary enzymolysis solution into a centrifuge and performing a centrifugation to obtain a centrifuge; J. Adding the enzymatic hydrolysis solution into the centrifugal solution again for enzymatic hydrolysis, and then performing a second centrifugation, and filtering to obtain peony flower cell water.

[0078] In this embodiment, the pretreatment method in step B is as follows: a. Rinse the peony flowers in clean water for 4 times and dry them at room temperature; b. Then immerse the cleaned peony flowers in the ethanol solution for 14 minutes, take them out, rinse them with clean water again, and dry them at room temperature.

[0079] In this embodiment, in step C, the processed peony flowers are added to a shearing machine and sheared into pieces with a side length of 13 mm.

[0080] In this embodiment, in step D, ultrasonication is performed while squeezing the peony flowers, and the ultrasonic frequency is 25 kHz and the time is 35 minutes.

[0081] In this embodiment, the first distillation temperature in step E is 85° C. and the time is 25 min; the second distillation temperature in step G is 90° C. and the time is 15 min.

[0082] In this embodiment, the enzymatic hydrolysate components include 4 parts of α-amylase, 3 parts of β-amylase, 3 parts of pullulanase, 4 parts of hemicellulase, and 4 parts of alkaline protease by weight. The enzymatic hydrolysate used in the present invention can release the active substances in the peony flower to the greatest extent, and further increase the content of effective ingredients in the cell water.

[0083] In this embodiment, the centrifugal speed in step I is 3000 rpm and the centrifugal time is 20 min.

[0084] In this embodiment, the secondary centrifugation rate in step J is 4000 rpm and the time is 25 min.

[0085] Comparative Example: A process for extracting peony flower cell water from peony flowers, the extraction process comprising the following steps: A. First, pick fresh peony flowers; B, pre-treating the picked peony flowers; C, shearing the pretreated peony flowers; D. Add the sheared peony flowers into a squeezer, add deionized water for squeezing, and filter to obtain a pressed residue and a filtrate; E. adding the pressed slag into a distillation tank for primary distillation, filtering, and obtaining a primary distillate; F. Mixing the filtrate and the primary distillate, adding the mixture into a reactor, and adding an enzymolysis solution for enzymolysis to obtain a primary enzymolysis solution; G. After filtering the primary enzymatic solution, the filtered solution is added to a distillation tank for secondary distillation, filtered, and a secondary distillate is obtained; H. adding enzymolysis solution to the secondary distilled liquid for enzymolysis treatment to obtain a secondary enzymolysis solution; 1. Adding the secondary enzymolysis solution into a centrifuge and performing a centrifugation to obtain a centrifuge; J. Adding the enzymatic hydrolysis solution into the centrifugal solution again for enzymatic hydrolysis, and then performing a second centrifugation, and filtering to obtain peony flower cell water.

[0086] In this embodiment, the pretreatment method in step B is as follows: a. Rinse the peony flowers in clean water for 3 times and dry them at room temperature; b. Then immerse the cleaned peony flowers in the ethanol solution for 10 minutes, take them out, rinse them with clean water again, and dry them at room temperature.

[0087] In this embodiment, in step C, the processed peony flowers are added to a shearing machine and cut into pieces with a side length of 10 mm.

[0088] In this embodiment, in step D, ultrasonication is performed while squeezing the peony flowers, and the ultrasonic frequency is 20 kHz and the time is 30 minutes.

[0089] In this embodiment, the first distillation temperature in step E is 75° C. and the time is 20 min; the second distillation temperature in step G is 85° C. and the time is 10 min.

[0090] In this embodiment, the centrifugal speed in step I is 2000 rpm and the centrifugal time is 15 min.

[0091] In this embodiment, the secondary centrifugation rate in step J is 3000 rpm and the time is 20 min.

[0092] Experimental example: The cell water prepared by each embodiment of the present invention was used to perform performance tests, and the data obtained are as follows: The steps of distilling, enzymolysis, centrifuging once, enzymolysis again and centrifuging twice on the peony flowers in the extraction process of the present invention can improve the content and purity of cell water.

[0093] In summary, the extraction process of the present invention is simple, the extracted cell water does not contain impurity ions and harmful substances, and retains the fragrance components, heat-sensitive substances and other active ingredients in the peony petals. The cell water is added to cosmetics to effectively improve the activity of the cosmetics.

[0094] As described above, the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A process for extracting peony flower cell water from peony flowers, characterized in that: The extraction process includes the following steps: A. First, pick fresh peony flowers; B, pre-treating the picked peony flowers; C, shearing the pretreated peony flowers; D. Add the sheared peony flowers into a squeezer, add deionized water for squeezing, and filter to obtain a pressed residue and a filtrate; E. adding the pressed slag into a distillation tank for primary distillation, filtering, and obtaining a primary distillate; F. Mixing the filtrate and the primary distillate, adding the mixture into a reactor, and adding an enzymolysis solution for enzymolysis to obtain a primary enzymolysis solution; G. After filtering the primary enzymatic solution, the filtered solution is added to a distillation tank for secondary distillation, filtered, and a secondary distillate is obtained; H. adding enzymolysis solution to the secondary distilled liquid for enzymolysis treatment to obtain a secondary enzymolysis solution; 1. Adding the secondary enzymolysis solution into a centrifuge and performing a centrifugation to obtain a centrifuge; J. Adding the enzymatic hydrolysis solution into the centrifugal solution again for enzymatic hydrolysis, and then performing a second centrifugation, and filtering to obtain peony flower cell water.

2. A process for extracting peony flower cell water from peony flowers according to claim 1, characterized in that: The pretreatment method in step B is as follows: a. Rinse the peony flowers in clean water for 3-5 times and dry them at room temperature; b. Then immerse the cleaned peony flowers in the ethanol solution for 10-18 minutes, take them out, rinse them with clean water again, and dry them at room temperature.

3. A process for extracting peony flower cell water from peony flowers according to claim 1, characterized in that: In the step C, the processed peony flowers are put into a shearing machine and sheared into pieces with a side length of 10 mm to 15 mm.

4. A process for extracting peony flower cell water from peony flowers according to claim 1, characterized in that: In the step D, the peony flower is squeezed and ultrasonicated simultaneously, the ultrasonic frequency is 20-30 kHz, and the time is 30 min-40 min.

5. A process for extracting peony flower cell water from peony flowers according to claim 1, characterized in that: In step E, the primary distillation temperature is 75°C-90°C, and the time is 20min-30min; in step G, the secondary distillation temperature is 85°C-95°C, and the time is 10min-20min.

6. A process for extracting peony flower cell water from peony flowers according to claim 1, characterized in that: The enzymatic hydrolysate components include 2-5 parts of alpha-amylase, 1-4 parts of beta-amylase, 1-4 parts of pullulanase, 2-6 parts of hemicellulase and 2-6 parts of alkaline protease by weight.

7. A process for extracting peony flower cell water from peony flowers according to claim 1, characterized in that: In the step I, the centrifugal speed is 2000-4000 rpm, and the centrifugal time is 15 min-25 min.

8. A process for extracting peony flower cell water from peony flowers according to claim 1, characterized in that: In the step J, the secondary centrifugation rate is 3000-5000 rpm, and the time is 20 min-30 min.

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