Composition with skin repairing effect, application and essence

Abstract

The invention belongs to the technical field of skin repair, and discloses a composition with a skin repair effect, application of the composition and essence. Comprising 20-30 parts of aloe barbadensis leaf water, 8-12 parts of apple fruit cell culture extract, 2-5 parts of grape flower cell extract and 0.5-1 part of oriental cherry leaf extract. The aloe barbadensis leaf water, the apple fruit cell culture extract, the grape flower cell extract and the oriental cherry leaf extract are compounded, and the apple fruit cell culture further promotes supplement of dermal cells such as fibroblasts and the like under the provision of substances of the grape flower cell extract and the oriental cherry leaf extract; and the aloe barbadensis leaf water and the oriental cherry leaf extract promote formation of cuticle and repair of damaged skin in the process again, and the effect of synergistically improving the skin repair effect is generated under the condition of repairing the skin in multiple directions.

Description

Technical Field

[0001] The present invention relates to the technical field of skin repair, and in particular to a composition, use and essence having skin repairing effect. Background Art

[0002] For the current skin care market, skin repair products are crucial, mainly based on the following core reasons:

[0003] (1) Damage to the skin barrier. The skin barrier is the first line of defense of the skin and is composed of the stratum corneum and sebum membrane. It can prevent water loss and invasion of foreign substances. When the skin barrier is damaged, it will lead to excessive water loss, the skin will become sensitive and susceptible to external stimuli, and then cause a series of skin problems such as dryness, tightness, redness, etc.

[0004] (2) Skin aging. As we age, the collagen and elastic fibers in the skin gradually decrease, causing the skin to sag and wrinkles to form. Proper skin repair can slow down the aging process and keep the skin firm and shiny.

[0005] (3) External environmental stimulation. Daily ultraviolet radiation, environmental pollution, etc. can cause damage to the skin, leading to skin photoaging, dryness, sensitivity and dullness. Skin repair can help repair these skin problems caused by external environmental stimulation and restore the skin to a healthy state.

[0006] Therefore, skin repair is the key direction of skin care product research and development. Prior art 1: Chinese patent application 202411624692.7 discloses a soothing and repairing composition, which comprises, by mass percentage, 45% to 55% raspberry fruit extract solution, 40% to 50% acerola cherry extract solution, and 5% to 15% oat kernel extract powder. The combination of the three can enhance the soothing and repairing effect of raspberry fruit. In addition, a cosmetic is also disclosed, comprising 0.2 to 1.5 wt% of the above-mentioned composition.

[0007] The specification of prior art 1 states: "Raspberry fruit extract solution is rich in anthocyanins, polyphenols, flavonoids, vitamin C, vitamin E and other active substances, which have the effects of repairing skin barrier function, inhibiting skin inflammatory response, and calming excessive nerve response to achieve the effect of soothing sensitive skin"; it means that prior art 1 is based on raspberry fruit extract solution to achieve skin repair by repairing skin barrier function; it mainly improves the problem of dry and sensitive skin through the compounding of components.

[0008] However, it is not enough to only focus on skin barrier repair when it comes to skin repair. It has good short-term effects but does not provide in-depth skin repair. Frequent use of skin repair products is required over a long period of time. Therefore, it is necessary to conduct multi-faceted skin repair research to avoid the problem of skin condition being in long-term need of skin repair. Summary of the invention

[0009] One of the purposes of the present invention is to provide a composition with skin repairing effect. By compounding aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract and Tokyo cherry leaf extract, the composition can repair the skin from multiple aspects such as repairing the stratum corneum, replenishing fibroblasts and providing supporting structural materials for fibroblasts, thereby improving the skin repairing effect of the composition.

[0010] Another object of the present invention is to provide a use of a composition with skin repairing effect for preparing cosmetics. The composition with skin repairing effect is used to prepare cosmetics, which can effectively improve the skin repairing effect of the cosmetics.

[0011] At the same time, the present invention also provides an essence containing the composition with skin repairing effect of the present invention, which has excellent skin repairing effect.

[0012] To achieve the above object, the present invention provides a composition with skin repairing effect, which comprises, by weight, 20-30 parts of aloe vera leaf water, 8-12 parts of apple fruit cell culture extract, 2-5 parts of grape flower cell extract, and 0.5-1 part of Tokyo cherry leaf extract.

[0013] The acetylated polysaccharide components in aloe barbadensis leaf water can enhance the skin's resistance, accelerate the healing of damaged tissues, and bring immediate relief to the skin. In addition, it can effectively repair skin epidermal cells damaged by ultraviolet rays and promote rapid recovery of sunburned skin. The various active ingredients in aloe barbadensis leaf water can accelerate the self-repair process of damaged skin.

[0014] Apple fruit cell culture extract can continuously renew the outermost epidermis, making the skin smooth and healthy. Human stem cells have the effect of replenishing dermal cells such as fibroblasts and helping wound healing, while apple fruit cell culture extract can provide important nutrients for human stem cells and improve the activity and health of human stem cells, effectively protecting and maintaining the efficacy of skin stem cells. Therefore, apple fruit cell culture extract can effectively promote the replenishment of dermal cells such as fibroblasts and promote wound healing.

[0015] Grape flower cell extract has the effect of increasing the proportion of collagen and promoting the synthesis of proteoglycans. After the skin is damaged, the dermis will produce new skin tissue through fibroblasts in a short period of time. These cells produce two substances that are indispensable for the skin's supporting structure: collagen and proteoglycans; therefore, grape flower cell extract can effectively provide fibroblasts with the substances needed for the supporting structure, effectively promoting skin repair;

[0016] Certain ingredients in Tokyo cherry leaf extract, such as sakura acid and sakura alkaloids, can promote the formation of stratum corneum and repair damaged skin; and Tokyo cherry leaf extract can promote the regeneration of collagen in the skin, achieving the purpose of firming the skin and delaying aging.

[0017] The present invention produces a synergistic effect of improving skin repair efficacy by compounding aloe barbadensis leaf water, apple fruit cell culture extract, grape flower cell extract and Tokyo cherry leaf extract. It is preliminarily judged that its synergistic mechanism should be: the apple fruit cell culture further promotes the supplement of dermal cells such as fibroblasts under the material provision of grape flower cell extract and Tokyo cherry leaf extract, and the aloe barbadensis leaf water and Tokyo cherry leaf extract promote the formation of the stratum corneum and repair damaged skin in the process again, and the skin is repaired in multiple directions, resulting in a synergistic effect of improving skin repair efficacy.

[0018] Preferably, the composition having skin repairing effect of the present invention further comprises a solvent.

[0019] Further preferably, the solvent includes glycerin, butylene glycol, water and phenoxyethanol.

[0020] Furthermore, the composition having skin repairing effect also includes lecithin and xanthan gum.

[0021] Preferably, the Tokyo cherry leaf extract is extracted by the following steps:

[0022] Step 1: Add Tokyo cherry leaf powder to an ethanol solution with an ethanol mass fraction of 40-60% at a solid-liquid ratio of 35 ml / g, then add 5-8% of the weight of Tokyo cherry leaf powder and organic acid, extract at an extraction temperature of 60-70°C for 35-45 minutes, and separate to obtain a filtrate;

[0023] Step 2: subjecting the filtrate to ethanol precipitation for 12-14 hours to obtain an alcohol precipitation solution, and then concentrating the solution to obtain an alcohol-free concentrate;

[0024] Step 3: Add an extractant to the alcohol-free concentrate to extract and obtain an extract, namely, Tokyo cherry leaf extract.

[0025] Preferably, in step 2, those skilled in the art may perform alcohol precipitation according to the existing alcohol precipitation process; more preferably, the specific steps of step 2 are: first, the filtrate is concentrated to 20% of the original mass using a single-effect concentrator, and then an equal mass of 90% ethanol solution is added and allowed to stand for 12-14 hours, centrifuged, and the supernatant is taken to obtain an alcohol precipitate, and the alcohol precipitate is transferred to a falling film concentrator, and the filtrate is vacuum concentrated to zero ethanol content at a concentration temperature of 50-60° C. and a concentration pressure of -0.07 MPa to -0.09 MPa to obtain an alcohol-free concentrate.

[0026] Preferably, the organic acid is one of tartaric acid, citric acid and ascorbic acid.

[0027] Preferably, the extractant is at least one of water, butylene glycol, glycerol and methylpropanediol, the amount of the extractant added is 1 to 5 times the weight of Tokyo cherry leaf powder, and the extraction time is 30 to 50 minutes.

[0028] In order to further increase the content of sakura alkaloids in Tokyo cherry leaf extract, the present invention discloses a method for extracting Tokyo cherry leaf extract, which improves the extraction efficiency of sakura alkaloids by adding organic acid into the extraction system, while not affecting the extraction content of other components in Tokyo cherry leaf extract; the Tokyo cherry leaf extract obtained by the extraction method is compounded with aloe vera leaf water, apple fruit cell culture extract, and grape flower cell extract to achieve a more significant synergistic effect.

[0029] The invention also discloses a use of the composition with skin repairing effect in preparing cosmetics.

[0030] At the same time, the invention also discloses an essence liquid containing 0.5-20% of the above composition with skin repairing effect.

[0031] Beneficial Effects

[0032] Compared with the prior art, the present invention has at least the following advantages:

[0033] (1) Apple fruit cell culture extract can continuously renew the outermost epidermis, making the skin smooth and healthy. Human stem cells have the effect of replenishing dermal cells such as fibroblasts and helping wound healing. Apple fruit cell culture extract can provide important nutrients for human stem cells and improve the activity and health of human stem cells, effectively protecting and maintaining the efficacy of skin stem cells. Therefore, apple fruit cell culture extract can effectively promote the replenishment of dermal cells such as fibroblasts and promote wound healing.

[0034] (2) The acetylated polysaccharide components in aloe barbadensis leaf water can enhance the skin's resistance, accelerate the healing of damaged tissues, and bring immediate soothing sensation to the skin. In addition, it can effectively repair skin epidermal cells damaged by ultraviolet rays and promote the rapid recovery of sunburned skin. The various active ingredients in aloe barbadensis leaf water can accelerate the self-repair process of damaged skin.

[0035] (3) Grape flower cell extract has the effect of increasing the proportion of collagen and promoting the synthesis of proteoglycans. After the skin is damaged, the dermis will produce new skin tissue through fibroblasts in a short period of time. These cells produce two substances that are indispensable for the skin's supporting structure: collagen and proteoglycans; therefore, grape flower cell extract can effectively provide fibroblasts with the substances needed for the supporting structure, effectively promoting skin repair;

[0036] (4) Certain ingredients in Tokyo cherry leaf extract, such as sakura acid and sakura alkaloids, can promote the formation of the stratum corneum and repair damaged skin; and Tokyo cherry leaf extract can promote the regeneration of collagen in the skin, thereby achieving the purpose of firming the skin and delaying aging.

[0037] (5) The present invention is compounded by aloe barbadensis leaf water, apple fruit cell culture extract, grape flower cell extract and Tokyo cherry leaf extract, wherein the apple fruit cell culture further promotes the replenishment of dermal cells such as fibroblasts with the substances provided by the grape flower cell extract and Tokyo cherry leaf extract, and the aloe barbadensis leaf water and Tokyo cherry leaf extract promote the formation of the stratum corneum and the repair of damaged skin in the process, thereby repairing the skin in multiple directions and producing a synergistic effect of enhancing the skin repair effect. DETAILED DESCRIPTION

[0038] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0039] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0040] In the following examples and comparative examples, unless otherwise specified, the aloe vera leaf water is purchased from Yunnan Wanlv Biological Co., Ltd., with the trade name of Wanlv brand aloe vera leaf water A04-3, and the aloe vera leaf water content is 100wt%; the apple fruit cell culture extract is purchased from Guangzhou Lingyu Trading Co., Ltd., with the trade name of apple stem cells, and the composition is: 88.06wt% water, 9wt% apple fruit cell culture extract, 0.14wt% lecithin, 0.4wt% glycerol, 1.4wt% phenoxyethanol, and 1wt% xanthan gum; the grape flower cell extract is purchased from NAOLYS, with the trade name of Healthy Perfection (Vitis flower)2S-Gly, composition: grape flower cell extract 20wt%, glycerol 80wt%; Tokyo cherry leaf extract was purchased from Japan Ichimaru Natural Beauty Co., Ltd., with the trade name Tokyo cherry leaf extract, and the composition: 49wt% butanediol, 49wt% water, and 2wt% Tokyo cherry leaf extract; Portulaca oleracea extract was purchased from Lanzhou Waters Biotechnology Co., Ltd., and the properties were powder; Alpine edelweiss callus extract was purchased from Guangzhou Huayang Biotechnology Co., Ltd., with the trade name edelweiss fermentation, components: 78wt% butanediol, 20wt% alpine edelweiss callus extract, and 2wt% filtrate of Leuconostoc / radish root fermentation product; Olive leaf extract was purchased from Guangzhou Huayang Biotechnology Co., Ltd., with the trade name Olive leaf fermentation, components: 54.4wt% water, 2wt% lactobacillus, and 43.5wt% olive leaf extract; Centella asiatica extract was purchased from Xi'an Anao Biotechnology Co., Ltd., with the item number: QAD-5803, and the properties were powder.

[0041] The technical solution of the present invention can be implemented by other commercially available products, and is not limited to the raw materials from the above sources.

[0042] Unless otherwise specified, in the following examples and comparative examples, the parts and % refer to parts by weight and percentage by weight, respectively.

[0043] According to the formula table in Table 1, the composition is prepared as follows:

[0044] Grape flower cell extract or olive leaf extract, aloe vera leaf water or purslane extract, Tokyo cherry leaf extract or Centella asiatica extract, apple fruit cell culture extract or alpine edelweiss callus extract are added into 80 parts of deionized water and stirred until completely dissolved.

[0045] It should be noted that in the following examples and comparative examples, deionized water is the solvent. The main purpose is to mix grape flower cell extract or olive leaf extract, aloe vera leaf water or purslane extract, Tokyo cherry leaf extract or Centella asiatica extract, apple fruit cell culture extract or alpine edelweiss callus extract, which has no effect on the efficacy of the composition.

[0046] It should be noted that the Tokyo cherry leaf extract in Example 5 and Example 6 in Table 1 was extracted by the following steps:

[0047] Step 1: Add Tokyo cherry leaf powder to an ethanol solution with a mass fraction of 60% ethanol at a solid-liquid ratio of 35 ml / g, then add citric acid with a mass fraction of 7% of the Tokyo cherry leaf powder, extract at an extraction temperature of 60°C for 45 minutes, and separate to obtain a filtrate;

[0048] Step 2: First, the filtrate is concentrated to 20% of the original mass using a single-effect concentrator, and then an equal mass of 90% ethanol solution is added and allowed to stand for 12-14 hours, centrifuged, and the supernatant is taken to obtain an alcohol precipitate, and the alcohol precipitate is transferred to a falling film concentrator, and the filtrate is vacuum concentrated to zero ethanol content at a concentration temperature of 50° C. and a concentration pressure of -0.08 MPa to obtain an alcohol-free concentrate;

[0049] Step 3: Add 3 times the weight of Tokyo cherry leaf powder butanediol to the alcohol-free concentrate and extract for 45 minutes to obtain an extract, namely, Tokyo cherry leaf extract.

[0050] Table 1 Composition formula

[0051]

[0052]

[0053] Efficacy Test

[0054] 1. Single component efficacy test

[0055] Test method:

[0056] Fibroblast scratch assay

[0057] 1.1 Experimental Setup

[0058] Component test: The sample was diluted with pure water to a solution with a volume fraction of 4%, and then filtered with a 0.22 μm filter, and the filtrate was collected as the sample mother solution.

[0059] Negative control: basic culture medium (cell activity test); incomplete culture medium (scratch test).

[0060] Positive control: basal culture medium.

[0061] 1.2 Experimental methods

[0062] Fibroblast scratch test: After fibroblasts were plated on a 6-well plate and cultured for 24 hours, a line was drawn in the middle of the 6-well plate with a sterile pipette tip to produce a scratch, and the culture medium was discarded. The basal culture medium (positive control), incomplete culture medium (negative control), and culture medium of the corresponding sample group were added respectively, and the cell healing was observed at 0h and 24h, and photos were taken.

[0063] 1.3 Calculation formula

[0064]

[0065] 1.4 Data Analysis

[0066] Image-pro plus (IPP) image analysis software was used to quantitatively analyze the cell scratch images at 0h and 24h.

[0067] Grape flower cell extract, olive leaf extract, aloe vera leaf water, purslane extract, Tokyo cherry leaf extract used in Examples 1-4, Tokyo cherry leaf extract used in Examples 5-6, Centella asiatica extract, apple fruit cell culture extract, and alpine edelweiss callus extract were tested according to the above test method, and the results are shown in Table 2;

[0068] Table 2 Results of single component fibroblast scratch test

[0069]

[0070] 2. Composition efficacy test

[0071] Test method:

[0072] Fibroblast scratch assay

[0073] 2.1 Experimental Setup

[0074] Composition test: The sample was diluted with pure water to a solution with a volume fraction of 4%, and then filtered with a 0.22 μm filter, and the filtrate was collected as the sample mother solution.

[0075] Negative control: basic culture medium (cell activity test); incomplete culture medium (scratch test).

[0076] Positive control: basal culture medium.

[0077] 2.2 Experimental methods

[0078] Fibroblast scratch test: After fibroblasts were plated on a 6-well plate and cultured for 24 hours, a line was drawn in the middle of the 6-well plate with a sterile pipette tip to produce a scratch, and the culture medium was discarded. The basal culture medium (positive control), incomplete culture medium (negative control), and culture medium of the corresponding sample group were added respectively, and the cell healing was observed at 0h and 24h, and photos were taken.

[0079] 2.3 Calculation formula

[0080]

[0081] 2.4 Data Analysis

[0082] Image-pro plus (IPP) image analysis software was used to quantitatively analyze the cell scratch images at 0h and 24h.

[0083] The compositions of Examples 1-4 and Comparative Examples 1-8 were tested according to the above test method, and the results are shown in Table 3;

[0084] Table 3 Fibroblast scratch test results of the compositions of Examples 1-4 and Comparative Examples 1-8

[0085]

[0086] According to the results in Table 2 and Table 3:

[0087] According to the data of Examples 1-6, the present invention has excellent skin repairing effect by compounding aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract and Tokyo cherry leaf extract;

[0088] The comparison of the data of Example 3 and Example 4 shows that the increase in the amount of apple fruit cell culture extract and grape flower cell extract can further improve the skin repair effect;

[0089] At the same time, according to the data comparison between Example 5 and Example 3, and the data comparison between Example 6 and Example 4, it can be seen that the Tokyo cherry leaf extract obtained by the specific extraction method of the present invention can significantly improve the skin repair effect of the composition. The reason is that: the present invention improves the extraction efficiency of sakura alkaloids by adding organic acids to the extraction system, while not affecting the extraction content of other components in the Tokyo cherry leaf extract, so that the extracted Tokyo cherry leaf extract has a higher content of sakura alkaloids. It is speculated that in the technical scheme of the present invention, sakura alkaloids play a very important role in the compound synergy with aloe vera leaf water, apple fruit cell culture extract, and grape flower cell extract;

[0090] At the same time, according to the data comparison of Example 5 and Example 6, after using the Tokyo cherry leaf extract obtained by the specific extraction step of the present invention, the difference in the 24h scratch healing rate of fibroblasts in Example 5 and Example 6 is not as large as the difference between Example 3 and Example 4, and the difference in the middle should be the influence of the difference in Tokyo cherry leaf extracts. This further proves that the Tokyo cherry leaf extract obtained by the specific step of the present invention can further enhance the effect of the combination of Tokyo cherry leaf extract and aloe vera leaf water, apple fruit cell culture extract, and grape flower cell extract to synergistically enhance the skin repair effect of the composition.

[0091] According to the data comparison between Example 4 and Comparative Examples 1-4, it can be seen that in the case where Comparative Examples 1-4 lack any one of Tokyo Sakura leaf extract, Aloe Barbadensis leaf water, Apple fruit cell culture extract, and Grape flower cell extract, even if the total amount of components of the composition is the same, the skin repair effect produced is significantly weaker than that of Example 4, and depending on the missing components, the skin repair effects between Comparative Examples 1-4 also have obvious differences, indicating that the present invention uses Aloe Barbadensis leaf water, Apple fruit cell culture extract, Grape flower cell extract and Tokyo Sakura leaf extract to produce a synergistic effect of enhancing the skin repair effect of the composition; while the lack of one of the components can only produce a simple superposition of component effects.

[0092] According to the comparison of the data in Table 2 and the data in Example 4 and Comparative Example 5 in Table 3, it can be seen that when the Portulaca oleracea extract with a higher 24-hour scratch healing rate of fibroblasts is used in Comparative Example 5 to replace the Aloe Barbadensis Leaf Water, the 24-hour scratch healing rate of fibroblasts exhibited by the resulting composition is significantly lower than that of Example 4. This further illustrates that the present invention uses the compound of Aloe Barbadensis Leaf Water, Apple Fruit Cell Culture Extract, Grape Flower Cell Extract and Tokyo Sakura Leaf Extract to synergistically enhance the skin repair efficacy of the composition.

[0093] Similarly, according to the comparison of the data in Table 2 and the data in Table 3 of Example 4 and Comparative Example 6, it can be seen that Comparative Example 6 uses the alpine edelweiss callus extract with a higher fibroblast 24h scratch healing rate to replace the apple fruit cell culture extract, and the resulting composition shows a fibroblast 24h scratch healing rate that is significantly lower than that of Example 4, which further verifies that the present invention uses the compound of aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract and Tokyo cherry leaf extract to synergistically enhance the skin repair efficacy of the composition.

[0094] According to the comparison of the data in Table 2 and the data in Table 3 of Example 4 and Comparative Examples 7 and 8, it can be seen that Comparative Example 7 uses olive leaf extract with a lower 24-hour scratch healing rate of fibroblasts to replace grape flower cell extract, and Comparative Example 8 uses Centella asiatica extract with a lower 24-hour scratch healing rate of fibroblasts to replace Tokyo cherry leaf extract. However, it can be seen from the data in Table 2 that the difference in 24-hour scratch healing rate of fibroblasts between olive leaf extract and grape flower cell extract, and between Centella asiatica extract and Tokyo cherry leaf extract is not huge. However, after forming the composition, the 24-hour scratch healing rate of fibroblasts shown by Comparative Examples 7 and Comparative Examples 8 is much weaker than that of Example 4, indicating that the present invention uses the compound of aloe vera leaf water, apple fruit cell culture extract, grape flower cell extract and Tokyo cherry leaf extract to synergistically enhance the skin repair effect of the composition.

[0095] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. A composition having a skin repairing effect, characterized in that: The invention comprises, by weight, 20-30 parts of aloe vera leaf water, 8-12 parts of apple fruit cell culture extract, 2-5 parts of grape flower cell extract and 0.5-1 part of Tokyo cherry leaf extract.

2. The composition having skin repairing effect according to claim 1, characterized in that: Also included are solvents.

3. The composition having skin repairing effect according to claim 1, characterized in that: The Tokyo cherry leaf extract is extracted by the following steps: Step 1: Add Tokyo cherry leaf powder to an ethanol solution with a mass fraction of ethanol of 40-60%, then add 5-8% of the weight of Tokyo cherry leaf powder and an organic acid, extract at an extraction temperature of 60-70° C. for 35-45 minutes, and separate to obtain a filtrate; Step 2: subjecting the filtrate to ethanol precipitation for 12-14 hours to obtain an alcohol precipitation solution, and then concentrating the solution to obtain an alcohol-free concentrate; Step 3: Add an extractant to the alcohol-free concentrate to extract and obtain an extract, namely, Tokyo cherry leaf extract.

4. Use of the composition with skin repairing effect as claimed in any one of claims 1 to 3 in preparing cosmetics.

5. An essence, characterized in that: The composition contains 0.5-20% of the composition having skin repairing effect as claimed in any one of claims 1-3.

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