Anti-aging composition and preparation method thereof

Abstract

The invention belongs to the technical field of medical preparations, and particularly relates to an anti-aging composition and a preparation method thereof. The anti-aging composition is prepared from the following raw materials in parts by weight: 5 to 10 parts of bovine collagen tripeptide, 5 to 10 parts of erythritol, 1 to 5 parts of concentrated apple juice, 0.05 to 1 part of hydrolyzed collagen, 0.05 to 1 part of trout essence powder, 0.05 to 1 part of fish maw oligopeptide, 0.05 to 1 part of szechwan chinaberry fruit powder, 0.05 to 1 part of brown sugar powder, 0.05 to 1 part of albumin polypeptide, 0.01 to 0.05 part of sucralose, 1 to 5 parts of anhydrous citric acid and 55 to 90 parts of purified water. According to the present invention, the collagen required by the human body can be supplemented, the skin elasticity can be improved, and the free radical can be removed.

Description

Technical Field

[0001] The invention belongs to the technical field of medical preparations, and specifically relates to an anti-aging composition and a preparation method thereof. Background Art

[0002] Collagen is the most abundant fibrous protein in the human body, accounting for about 25% of the total body protein. It is the main component of skin, bones, cartilage, hair, fingernails and toenails. Many studies have confirmed that collagen has the dual functions of maintaining skin moisture and regulating skin elasticity. Not all collagen can be effectively absorbed and utilized. The collagen that the skin really needs is actually a type of collagen composed of three amino acid molecules, namely "collagen tripeptide".

[0003] The advantages of collagen tripeptide are as follows: 1. The molecular weight of collagen is very large (100,000-300,000 Daltons), and the human body can hardly digest and absorb it directly; collagen peptides are composed of 30-50 amino acids. After entering the body, they are randomly synthesized into proteins or collagen in various parts of the body, and the absorption rate is also low; while the molecular weight of collagen tripeptide is only 250-1000 Daltons, which can be directly absorbed and utilized by the small intestine quickly and efficiently. Under the premise of taking the same amount, the absorption rate is higher than that of ordinary collagen peptides, which can reach more than 95%.

[0004] 2. Collagen tripeptide has good skin permeability and can be directly transported to skin fibroblasts through the blood. It can be absorbed and utilized by the skin without being decomposed. GPH in collagen tripeptide can be deposited in the skin in a targeted manner, stimulating dermal fibroblasts to produce collagen in the skin and activating the hyaluronic acid synthesis factor HAS2 (hyaluronansynthase 2), which is equivalent to replenishing collagen and hyaluronic acid at the same time, repairing skin cells and replenishing moisture.

[0005] 3. Collagen tripeptide is colorless, odorless, pH stable and temperature stable. Experiments have shown that after adding collagen tripeptide, the active ingredient GPH is not decomposed by gastric acid and pepsin for up to 2 hours, showing its high stability.

[0006] Chinese patent CN 113520900 A discloses a hypoallergenic, anti-aging yak collagen composition and its application, wherein the composition comprises the following components in percentage by mass: 1-5% yak collagen, 0.1-1.0% dipotassium glycyrrhizinate, 0.5-5% white willow bark extract, 0.5-3% ellagic acid, 0.5-3% resveratrol, 0.1-0.3% acetyl hexapeptide-8, 1-3% glycerol, 0.1-1.0% vitamin E, 0.5-1.0% hyaluronic acid, 0.05-0.2% methylparaben, and 0.01-0.14% propylparaben.

[0007] Chinese patent CN 113876609 A discloses an anti-aging composition containing hydroxypropyl tetrahydropyrantriol and polypeptide protein and its application. In parts by weight, the anti-aging composition includes 0.1-20 parts by weight of hydroxypropyl tetrahydropyrantriol, 0.1-20 parts by weight of snake venom peptide, 0.1-20 parts by weight of recombinant human-like type III collagen, 5-30 parts by weight of butanediol and 20-70 parts by weight of deionized water.

[0008] The collagen used in the above patent cannot be effectively absorbed and utilized by the human body, and its anti-aging effect is limited. Summary of the invention

[0009] The purpose of the present invention is to provide an anti-aging composition, which can not only supplement the collagen required by the human body, but also improve skin elasticity and eliminate free radicals; the present invention also provides a preparation method of the anti-aging composition.

[0010] The anti-aging composition of the present invention is made of the following raw materials in parts by weight: 5-10 parts of bovine collagen tripeptide, 5-10 parts of erythritol, 1-5 parts of concentrated apple juice, 0.05-1 parts of hydrolyzed collagen, 0.05-1 parts of salmon powder, 0.05-1 parts of fish maw oligopeptide, 0.05-1 parts of golden bell powder, 0.05-1 parts of brown sugar powder, 0.05-1 parts of albumin polypeptide, 0.01-0.05 parts of sucralose, 1-5 parts of anhydrous citric acid, 55-90 parts of purified water.

[0011] The anti-aging composition of the present invention is preferably made of the following raw materials in parts by weight: 6 parts of bovine collagen tripeptide, 7 parts of erythritol, 3 parts of concentrated apple juice, 0.1 parts of hydrolyzed collagen, 0.08 parts of salmon powder, 0.1 parts of fish maw oligopeptide, 0.05 parts of golden bell powder, 0.05 parts of brown sugar powder, 0.1 parts of albumin polypeptide, 0.02 parts of sucralose, 3 parts of anhydrous citric acid, and 80.5 parts of purified water.

[0012] The method for preparing the anti-aging composition of the present invention comprises the following steps: (1) heating a portion of purified water to obtain preheated purified water; (2) Mix salmon powder, fish maw oligopeptide and anhydrous citric acid and dissolve them in preheated purified water, apply an electric field, add brown sugar powder and mix, and finally add bovine collagen tripeptide, erythritol, concentrated apple juice, hydrolyzed collagen, golden bell fruit powder, albumin polypeptide, sucralose and the remaining purified water and mix evenly to obtain a mixed solution; (3) Filter, fill and sterilize the mixed solution in sequence.

[0013] The heating temperature in step (1) is 50-60° C.; the mass of the purified water is 40-60% of the total mass of the purified water, preferably 50%.

[0014] The voltage of the electric field in step (2) is 50-100 V, and the time for applying the electric field is 20-30 min.

[0015] The filter mesh used in the filtration in step (3) has a size of 200-400 mesh, preferably 200 mesh.

[0016] The sterilization pressure in step (3) is 1-2 bar, preferably 1.6 bar; the sterilization temperature is 115-130° C., preferably 115° C.; and the sterilization time is 20-45 min, preferably 40 min.

[0017] The raw materials of the anti-aging composition of the present invention also include 0.05-1 part of sea buckthorn juice, 0.05-1 part of acai juice, 0.05-1 part of pomegranate concentrated powder, 0.05-1 part of Paecilomyces aviculare mycelium powder and 0.05-0.1 part of mixed fruit essence, calculated in parts by weight; preferably 0.05 part of sea buckthorn juice, 0.12 part of acai juice, 0.08 part of pomegranate concentrated powder, 0.08 part of Paecilomyces aviculare mycelium powder and 0.1 part of mixed fruit essence.

[0018] The method for preparing the anti-aging composition of the present invention comprises the following steps: (1) heating a portion of purified water to obtain preheated purified water; (2) Mix salmon powder, fish maw oligopeptide and anhydrous citric acid and dissolve them in preheated purified water, apply an electric field, add brown sugar powder and mix, and finally add bovine collagen tripeptide, erythritol, concentrated apple juice, hydrolyzed collagen, golden bell fruit powder, albumin polypeptide, sucralose, sea buckthorn juice, acai juice, pomegranate concentrate powder, Paecilomyces pilosula mycelium powder, mixed fruit essence and the remaining purified water and mix evenly to obtain a mixed solution; (3) Filter, fill and sterilize the mixed solution in sequence.

[0019] The heating temperature in step (1) is 50-60° C.; the mass of the purified water is 40-60% of the total mass of the purified water, preferably 50%.

[0020] The voltage of the electric field in step (2) is 50-100 V, and the time for applying the electric field is 20-30 min.

[0021] The filter mesh used in the filtration in step (3) has a size of 200-400 mesh, preferably 200 mesh.

[0022] The sterilization pressure in step (3) is 1-2 bar, preferably 1.6 bar; the sterilization temperature is 115-130° C., preferably 115° C.; and the sterilization time is 20-45 min, preferably 40 min.

[0023] The anti-aging composition of the present invention is a beverage product.

[0024] The beneficial effects of the present invention are as follows: (1) The content of hydroxyproline (Hyp) in bovine collagen tripeptide is significantly higher than that in collagen tripeptide from other sources, and the triple helix structure is more stable. The product has a higher active ingredient content and a higher absorption rate by the human body.

[0025] (2) Hydrolyzed collagen has a smaller molecular weight and is easier for the human body to absorb.

[0026] (3) Concentrated apple juice can effectively neutralize the unique fishy smell of collagen tripeptide, making it more acceptable to people.

[0027] (4) The nucleoprotein in salmon powder has a direct antioxidant effect, and it can synergize with fish maw oligopeptide to enhance the antioxidant activity and enhance the activity and elasticity of human skin. However, if salmon powder and fish maw oligopeptide are taken directly, they are absorbed by the human body separately, and their synergistic antioxidant effect is not obvious. In the present invention, salmon powder, fish maw oligopeptide and anhydrous citric acid are first added to water, and the acidity of anhydrous citric acid is used to make salmon powder and fish maw oligopeptide carry positive charge under acidic conditions, and an electric field is applied to make salmon powder and fish maw oligopeptide aggregate together, and then brown sugar powder is added. After being added to water, brown sugar powder has viscosity, and salmon powder and fish maw oligopeptide are bonded together by brown sugar powder. After bonding, salmon powder and fish maw oligopeptide can be absorbed by the human body together, and the synergistic antioxidant effect is strong. The nucleoprotein in salmon powder contains amino and hydroxyl groups, while the amino acids in fish maw oligopeptide contain carboxyl groups. The amino and hydroxyl groups of the nucleoprotein in salmon powder and the carboxyl groups of the amino acids in fish maw oligopeptide not only attract each other, but also have intermolecular forces, forming a stable antioxidant system together. In addition, brown sugar powder contains a large amount of hydroxyl groups, which can further enhance the antioxidant capacity of salmon powder and fish maw oligopeptide.

[0028] (5) Albumin polypeptide contains 20 essential and non-essential amino acids required by the human body, and can supplement the various amino acids required by the body. At the same time, its composition ratio is very similar to that of human serum protein (albumin), and it can replace serum protein (albumin) to maintain plasma colloidal osmotic pressure and assist in the transport of various substances in the body; it can also accelerate the removal of free radicals, play an anti-aging role and slow down the formation of skin wrinkles.

[0029] (6) Pomegranate concentrate powder is rich in bioactive substances such as punicalagin and ellagic acid. It can not only effectively inhibit and remove glycation in the body and skin, and reduce the impact of glycation on the skin, but also prevent and reduce the degradation of collagen and cell apoptosis in the skin after ultraviolet radiation, thereby playing a role in moisturizing, anti-wrinkle, anti-inflammatory, whitening and other skin beautifying effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is the facial skin MI value result chart.

[0031] Figure 2 This is the result chart of facial skin EI value.

[0032] Figure 3 This is a graph showing the basal cell counts containing melanin granules in mouse basal cells. DETAILED DESCRIPTION

[0033] The present invention is further described below with reference to the embodiments.

[0034] Example 1 (1) heating 40.25 parts of purified water to 60° C. to obtain preheated purified water; (2) 0.08 parts of salmon powder, 0.1 parts of fish maw oligopeptide and 3 parts of anhydrous citric acid were mixed and dissolved in preheated purified water, and an electric field was applied. The voltage of the electric field was 80 V and the time of applying the electric field was 25 minutes. Then 0.05 parts of brown sugar powder were added and mixed. Finally, 6 parts of bovine collagen tripeptide, 7 parts of erythritol, 3 parts of concentrated apple juice, 0.1 parts of hydrolyzed collagen, 0.05 parts of golden bell fruit powder, 0.1 parts of albumin polypeptide, 0.02 parts of sucralose, 0.05 parts of sea buckthorn juice, 0.12 parts of acai juice, 0.08 parts of red pomegranate concentrated powder, 0.08 parts of Paecilomyces pyralis mycelium powder, 0.1 parts of mixed fruit essence and 40.25 parts of purified water were added and mixed evenly to obtain a mixed solution; (3) The mixed liquid is filtered, filled, and sterilized in sequence to obtain the product; wherein the filter mesh used in the filtration is 200 mesh; the sterilization pressure is 1.6 bar, the sterilization temperature is 115°C, and the sterilization time is 40 min.

[0035] Example 2 (1) heating 33 parts of purified water to 50° C. to obtain preheated purified water; (2) 0.1 parts of salmon powder, 1 part of fish maw oligopeptide and 2 parts of anhydrous citric acid were mixed and dissolved in preheated purified water, and an electric field was applied. The voltage of the electric field was 50 V and the time of applying the electric field was 30 minutes. Then 0.1 parts of brown sugar powder were added and mixed. Finally, 5 parts of bovine collagen tripeptide, 5 parts of erythritol, 5 parts of concentrated apple juice, 1 part of hydrolyzed collagen, 0.1 parts of golden bell fruit powder, 0.05 parts of albumin polypeptide, 0.01 parts of sucralose, 0.1 parts of sea buckthorn juice, 0.05 parts of acai juice, 1 part of red pomegranate concentrated powder, 1 part of Paecilomyces pyralis mycelium powder, 0.05 parts of mixed fruit essence and 22 parts of purified water were added and mixed evenly to obtain a mixed solution; (3) The mixed liquid is filtered, filled and sterilized in sequence to obtain the product; wherein the filter mesh used in the filtration is 300 mesh; the sterilization pressure is 1 bar, the sterilization temperature is 130°C, and the sterilization time is 20 min.

[0036] Example 3 (1) heating 36 parts of purified water to 55° C. to obtain preheated purified water; (2) 1 part of salmon powder, 0.2 part of fish maw oligopeptide and 1 part of anhydrous citric acid were mixed and dissolved in preheated purified water, and an electric field was applied with a voltage of 100 V for 20 min. Then 0.3 part of brown sugar powder was added and mixed. Finally, 7 parts of bovine collagen tripeptide, 8 parts of erythritol, 2 parts of concentrated apple juice, 0.3 parts of hydrolyzed collagen, 0.3 parts of golden bell fruit powder, 0.2 parts of albumin polypeptide, 0.03 parts of sucralose, 1 part of sea buckthorn juice, 1 part of acai juice, 0.05 parts of red pomegranate concentrated powder, 0.05 parts of Paecilomyces hymenii mycelium powder, 0.08 parts of mixed fruit essence and 54 parts of purified water were added and mixed evenly to obtain a mixed solution; (3) The mixed liquid is filtered, filled, and sterilized in sequence to obtain the product; wherein the filter mesh used in the filtration is 400 mesh; the sterilization pressure is 2 bar, the sterilization temperature is 120°C, and the sterilization time is 30 min.

[0037] Example 4 (1) heating 40 parts of purified water to 60° C. to obtain preheated purified water; (2) 0.05 parts of salmon powder, 0.05 parts of fish maw oligopeptide and 5 parts of anhydrous citric acid were mixed and dissolved in preheated purified water, and an electric field was applied. The voltage of the electric field was 70 V and the time of applying the electric field was 27 minutes. Then 1 part of brown sugar powder was added and mixed. Finally, 10 parts of bovine collagen tripeptide, 10 parts of erythritol, 1 part of concentrated apple juice, 0.05 parts of hydrolyzed collagen, 1 part of golden bell fruit powder, 1 part of albumin polypeptide, 0.05 parts of sucralose and 40 parts of purified water were added and mixed evenly to obtain a mixed solution; (3) The mixed liquid is filtered, filled and sterilized in sequence to obtain the product; wherein the filter mesh used in the filtration is 200 mesh; the sterilization pressure is 1.5 bar, the sterilization temperature is 115°C, and the sterilization time is 45 min.

[0038] Comparative Example 1 Without adding salmon powder, the other steps are the same as in Example 1.

[0039] Comparative Example 2 No fish maw oligopeptide was added, and other steps were the same as in Example 1.

[0040] Comparative Example 3 Without adding brown sugar powder, the other steps are the same as in Example 1.

[0041] Comparative Example 4 No electric field is applied, and the other steps are the same as in Example 1.

[0042] 1. Skin test experiment: Experimental subjects: 450 healthy women aged 18-60 years old were selected.

[0043] Dosage and usage of the test sample: 450 subjects were randomly divided into 9 groups, each with 50 subjects, namely, the control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group and Comparative Example 4 group. The control group had a normal diet; the Example 1 group, Example 2 group, Example 3 group and Example 4 group consumed the products of Example 1, Example 2, Example 3 and Example 4 respectively, and the Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group and Comparative Example 4 group consumed the products of Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 respectively, twice a day, 50 grams each time, and taken directly by drinking. During the trial period, the original eating habits were not changed, and the diet was normal.

[0044] Use the skin melanin and hemoglobin test probe Mexameter® MX18 to detect the melanin and hemoglobin content of the cheek skin. Test once before the experiment, and test the melanin and hemoglobin content of the cheek skin again after taking it for 56 consecutive days. The smaller the MI value, the lower the skin melanin content; the smaller the EI value, the lower the skin hemoglobin content. Test principle: Based on the simple reflection spectrophotometry, the specific absorption spectrum of melanin and hemoglobin (green light 568nm, red light 660nm, infrared light 880nm) is selected to irradiate the skin, and a built-in photoelectric detector receives the reflected light, and converts it into the content value of melanin and hemoglobin by calculating the amount of skin absorption and reflection.

[0045] Note: Before the test, the subjects should clean their faces and rest in a constant temperature and humidity room to ensure that the skin remains stable for 30 minutes before the test. During the test, the subjects should maintain normal skin care habits, not change new skin care products, and reduce outdoor strong sun exposure activities and not travel or go on vacation.

[0046] Experimental results: The results of facial skin MI value (skin melanin index) are shown in Figure 1 , the results of facial skin EI value (erythema index) are shown in Figure 2 .

[0047] Depend on Figure 1 It can be seen that taking the products of Examples 1-4 can reduce the skin MI value, and taking the products of Examples 1-4 is more effective in reducing the skin MI value than taking the products of Comparative Examples 1-4.

[0048] Depend on Figure 2 It can be seen that taking the products of Examples 1-4 can reduce the EI value of the skin, and taking the products of Examples 1-4 is more effective in reducing the EI value of the skin than taking the products of Comparative Examples 1-4.

[0049] The above results show that the anti-aging compositions prepared in Examples 1-4 can effectively improve skin condition.

[0050] 2. Mouse Experiment: Experimental subjects: 450 adult brown guinea pigs were selected.

[0051] Dosage and usage of the test sample: 450 guinea pigs were randomly divided into 9 groups, each with 50 guinea pigs, namely, the control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group and Comparative Example 4 group. The control group was fed normally; the Example 1 group, Example 2 group, Example 3 group and Example 4 group were fed with the products of Example 1, Example 2, Example 3 and Example 4, respectively; the Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group and Comparative Example 4 group were fed with the products of Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4, respectively, once a day, 20 grams each time, and the original feeding rules were not changed during the experiment, and the feeding was normal.

[0052] Before the experiment, a guinea pig skin part was selected and depilated, and UVB ultraviolet irradiation was performed. The UVB ultraviolet lamp had an irradiation wavelength of 310 nm. After a period of time to induce skin pigmentation in the corresponding part, the irradiation was stopped. The embodiment and comparative example products were fed once a day, 20 grams each time. On the 1st, 3rd, 6th, 9th, and 12th days, the skin biopsy sections of the guinea pigs were stained, and histological observations were performed, and optical density analysis was performed. The basal cells containing melanin granules in the basal cells were counted, and the average value was taken to calculate the percentage.

[0053] Experimental results: The basal cell counts containing melanin granules in mouse basal cells are shown in the figure Figure 3 .

[0054] Depend on Figure 3 It can be seen that with the passage of time, the proportion of basal cells containing melanin granules in the basal cells of mice in the control group continued to increase, and the proportion of basal cells containing melanin granules in the basal cells of mice in Example 1, Example 2, Example 3 and Example 4 continued to decrease; the proportion of basal cells containing melanin granules in the basal cells of mice in Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 also continued to decrease, but the degree of decrease was not as great as that in Example 1.

Claims

1. An anti-aging composition, characterized in that The invention is made of the following raw materials in parts by weight: 5-10 parts of bovine collagen tripeptide, 5-10 parts of erythritol, 1-5 parts of concentrated apple juice, 0.05-1 parts of hydrolyzed collagen, 0.05-1 parts of salmon powder, 0.05-1 parts of fish maw oligopeptide, 0.05-1 parts of golden bell powder, 0.05-1 parts of brown sugar powder, 0.05-1 parts of albumin polypeptide, 0.01-0.05 parts of sucralose, 1-5 parts of anhydrous citric acid, 55-90 parts of purified water.

2. The anti-aging composition according to claim 1, characterized in that The raw materials also include 0.05-1 part of sea buckthorn juice, 0.05-1 part of acai juice, 0.05-1 part of red pomegranate concentrated powder, 0.05-1 part of Paecilomyces hymenii mycelium powder and 0.05-0.1 part of mixed fruit essence, calculated by weight.

3. A method for preparing the anti-aging composition according to claim 1, characterized in that The steps include: (1) heating a portion of purified water to obtain preheated purified water; (2) Mix salmon powder, fish maw oligopeptide and anhydrous citric acid and dissolve them in preheated purified water, apply an electric field, add brown sugar powder and mix, and finally add bovine collagen tripeptide, erythritol, concentrated apple juice, hydrolyzed collagen, golden bell fruit powder, albumin polypeptide, sucralose and the remaining purified water and mix evenly to obtain a mixed solution; (3) Filter, fill and sterilize the mixed solution in sequence.

4. The method for preparing the anti-aging composition according to claim 3, characterized in that In step (1), the heating temperature is 50-60° C., and the mass of the purified water is 40-60% of the total mass of the purified water.

5. The method for preparing the anti-aging composition according to claim 3, characterized in that In step (2), the voltage of the electric field is 50-100 V, and the time for applying the electric field is 20-30 min.

6. The method for preparing the anti-aging composition according to claim 3, characterized in that The filter mesh used in the filtration in step (3) is 200-400 mesh; the sterilization pressure is 1-2 bar, the sterilization temperature is 115-130° C., and the sterilization time is 20-45 min.

7. A method for preparing the anti-aging composition according to claim 2, characterized in that The steps include: (1) heating a portion of purified water to obtain preheated purified water; (2) Mix salmon powder, fish maw oligopeptide and anhydrous citric acid and dissolve them in preheated purified water, apply an electric field, add brown sugar powder and mix, and finally add bovine collagen tripeptide, erythritol, concentrated apple juice, hydrolyzed collagen, golden bell fruit powder, albumin polypeptide, sucralose, sea buckthorn juice, acai juice, pomegranate concentrate powder, Paecilomyces pilosula mycelium powder, mixed fruit essence and the remaining purified water and mix evenly to obtain a mixed solution; (3) Filter, fill and sterilize the mixed solution in sequence.

8. The method for preparing the anti-aging composition according to claim 7, characterized in that In step (1), the heating temperature is 50-60° C., and the mass of the purified water is 40-60% of the total mass of the purified water.

9. The method for preparing the anti-aging composition according to claim 7, characterized in that In step (2), the voltage of the electric field is 50-100 V, and the time for applying the electric field is 20-30 min.

10. The method for preparing the anti-aging composition according to claim 7, characterized in that The filter mesh used in the filtration in step (3) is 200-400 mesh; the sterilization pressure is 1-2 bar, the sterilization temperature is 115-130° C., and the sterilization time is 20-45 min.

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