Extraction method and application of freeze-dried moringa seed polysaccharide

Abstract

The extraction method of the freeze-dried moringa seed polysaccharide comprises the following steps: (1) performing degreasing treatment on hulled and crushed moringa seed kernels to obtain degreased moringa seed meal; (2) preparing a natural eutectic solvent from betaine and 1, 3-butanediol; (3) moringa seed polysaccharide extraction: preparing a DESs solution, carrying out polysaccharide extraction on moringa seed meal to obtain moringa seed meal mixed homogenate, carrying out graded filtration on the moringa seed meal mixed homogenate by adopting a filter device to obtain a clear moringa seed polysaccharide extracting solution, and carrying out pressure reduction, concentration, drying, washing and filtration twice to obtain moringa seed polysaccharide; and (4) freeze-drying the moringa seed polysaccharide. According to the invention, a natural eutectic solvent extraction technology is combined with a freeze drying technology, the extraction technology of the moringa seed meal raw material is innovatively optimized, and a corresponding filtering device is matched, so that the extraction and separation efficiency of moringa seed polysaccharide is improved, and a new idea is provided for cosmetic raw materials while the deep development of the moringa seed industry is promoted.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a filtering device, an extraction method and an application thereof for extracting freeze-dried Moringa seed polysaccharide. Background Art

[0002] Moringa is native to India. As the fruit of Moringa, Moringa seeds are rich in plant oils and a variety of active substances, and have good edible and medicinal value. At present, the extraction methods of Moringa seed oil are commonly used, such as oil solvent extraction, enzymatic extraction, and ultrasonic assisted extraction, which have low extraction rates and the risk of solvent residues.

[0003] Moringa seed meal is a byproduct of moringa seed oil extraction. Studies have shown that it is rich in protein, polysaccharides and polyphenol compounds. Research data has shown that moringa seed polysaccharides have antioxidant, antibacterial, hypoglycemic, immune-regulating effects. As a natural plant-derived functional ingredient, it has potential development prospects in the cosmetics field. The current extraction method of plant polysaccharides is mostly water extraction, which is relatively simple, has a long extraction cycle and low efficiency, resulting in an increase in extraction costs and is not conducive to industrial scale-up production.

[0004] Currently, moringa seed meal is mostly processed as animal feed, which has problems such as relatively low economic value and insufficient resource utilization. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a filtering device, an extraction method and an application for extracting freeze-dried Moringa seed polysaccharides.

[0006] The present invention performs green extraction on moringa seed meal through a natural low eutectic solvent to obtain freeze-dried moringa seed polysaccharide, and applies the freeze-dried moringa seed polysaccharide to a cosmetic formula. The safety and effectiveness of the moringa seed polysaccharide are verified through in vitro efficacy tests, cell tests and human efficacy tests, and the by-products of moringa seed oil extraction are successfully utilized and the extraction process is optimized.

[0007] The invention discloses a filtration device for extracting freeze-dried Moringa seed polysaccharides, wherein five layers of filtration structures are fixed in sequence from top to bottom: a first filtration screen, a second filtration screen, a macroporous resin, an activated carbon filtration layer, and filter paper and a ceramic filter sheet layer.

[0008] The filtering device for extracting freeze-dried Moringa seed polysaccharides described in the present invention, wherein the first filter screen is 80 mesh, the second filter screen is 120 mesh, the macroporous resin is D101 macroporous resin, the thickness of the activated carbon filter layer is 5 mm, the filter paper and ceramic filter layer are 10ppi ceramic filter sheets, and a single layer of qualitative filter paper is arranged thereon.

[0009] The filtering device for extracting freeze-dried Moringa seed polysaccharides described in the present invention comprises five layers of filtering structures fixed on a filtering layer frame in sequence from top to bottom, a liquid collecting hopper is arranged at the lower part of the filtering layer frame, and is connected to the filter paper and the ceramic filter layer, the filtering layer frame and the liquid collecting hopper are fixed inside the filter cylinder wall, and a liquid collecting funnel, a liquid valve and a pressure regulating pipe are respectively arranged on the top, bottom and side of the filter cylinder wall; the liquid collecting funnel is connected to the internal space of the filtering layer frame, the liquid valve is connected to the bottom of the liquid collecting hopper, the upper and lower parts of the pressure regulating pipe are respectively connected to the upper space of the first filter screen and the upper space of the liquid collecting hopper, and a pressure regulating valve is arranged on the pressure regulating pipe.

[0010] The liquid collecting funnel, the filter cylinder wall, the filter layer frame, the first filter screen, the second filter screen, the liquid collecting bucket, the liquid valve, the pressure regulating pipeline and the pressure regulating valve are all made of 304 stainless steel.

[0011] A method for extracting freeze-dried Moringa seed polysaccharides comprises the following steps:

[0012] (1) degreasing the shelled and crushed Moringa seed kernels by supercritical carbon dioxide fluid extraction to obtain defatted Moringa seed meal;

[0013] (2) natural deep eutectic solvents, i.e., DESs, were prepared using betaine and 1,3-butanediol;

[0014] (3) Extraction of Moringa Seed Polysaccharide: Prepare a DESs solution, extract polysaccharide from Moringa seed meal, obtain a mixed slurry of Moringa seed meal, and grade filter the mixed slurry of Moringa seed meal using the filtering device of the present invention to obtain a clarified Moringa seed polysaccharide extract, and obtain Moringa seed polysaccharide after decompression, concentration, drying, washing and filtering twice;

[0015] (4) freeze-drying the Moringa seed polysaccharide to obtain the final product, Moringa seed polysaccharide freeze-dried powder.

[0016] The method for extracting freeze-dried Moringa seed polysaccharides of the present invention is characterized in that the supercritical carbon dioxide fluid extraction method comprises the following steps:

[0017] The moringa seeds are crushed and loaded into the extraction kettle, the carbon dioxide switch is turned on, and the pressure and temperature of the extraction kettle, the first separation kettle and the second separation kettle are adjusted. When the extraction pressure is 35MPa and the extraction temperature is 40°C, the pressure of the first separation kettle is 12MPa and the separation temperature is 45°C, and the pressure of the second separation kettle is 5MPa and the separation temperature is 35°C, the valve is adjusted to stabilize; the carbon dioxide fluid is fully in contact with the moringa seed material in the extraction kettle, and the carbon dioxide fluid carrying moringa seed oil is passed into the first separation kettle through a pipeline to separate the moringa seed oil for the first time, and then a small amount of unseparated moringa seed oil is allowed to enter the second separation kettle with the carbon dioxide fluid for secondary separation, and the separated carbon dioxide is circulated through a loop; when the preset pressure and temperature of the extraction kettle, the first separation kettle and the second separation kettle are stable for a period of time, the moringa seed oil is collected from the first separation kettle and the second separation kettle every 30 minutes; the overall extraction time is 3h, and after the extraction is completed, the moringa seed oil is obtained by merging, and the defatted moringa seed meal in the extraction kettle is collected.

[0018] The method for extracting freeze-dried Moringa seed polysaccharides described in the present invention, wherein step (2) specifically comprises the following steps: placing betaine and 1,3-butanediol in a beaker at a molar ratio of 1:1, heating and stirring at 80°C until a transparent and uniform liquid is formed, a natural low eutectic solvent, i.e., DESs, is cooled to room temperature and stored for later use.

[0019] The method for extracting freeze-dried Moringa seed polysaccharides of the present invention, wherein step (3) specifically comprises the following steps: uniformly mixing DESs with distilled water to prepare a DESs solution with a water content of 85%, extracting polysaccharides from Moringa seed meal at a solid-liquid ratio of Moringa seed meal to DESs solution of 1:5, extracting at a temperature of 70° C. for 120 min, and obtaining a mixed slurry of Moringa seed meal;

[0020] The mixed slurry of Moringa seed meal is graded and filtered through a filtering device to obtain a clarified Moringa seed polysaccharide extract, which is concentrated to 1 / 3 of the original volume by vacuum concentration and drying at a pressure of -0.1 MPa and a temperature of 50-60°C, and then anhydrous ethanol is added in a ratio of 1:3 between the volume of Moringa seed polysaccharide extract and anhydrous ethanol, and the mixture is precipitated with alcohol for 12 hours, and the supernatant is removed by high-speed centrifugation at 10000 r / min for 10 minutes, and water is added to dissolve the mixture, and the mixture is filtered with a single layer of qualitative filter paper, and vacuum concentration is performed at a pressure of -0.1 MPa and a temperature of 50-60°C, and the mixture is concentrated to 1 / 3 of the original volume, and anhydrous ethanol is added in a ratio of 1:3 between the volume of Moringa seed polysaccharide extract and anhydrous ethanol, and the mixture is precipitated with alcohol for 12 hours, and the Moringa seed polysaccharide is obtained by centrifugation, and the mixture is repeatedly washed with 95% anhydrous ethanol until the washing liquid is clarified, and the Moringa seed polysaccharide is obtained by filtration.

[0021] The method for extracting freeze-dried Moringa seed polysaccharides described in the present invention, wherein step (4) specifically comprises the following steps: pre-freezing the obtained Moringa seed polysaccharides at -80°C for 12 hours, and freeze-drying for 3-4 hours at -80°C and 0.37 mbar to finally obtain Moringa seed polysaccharide freeze-dried powder.

[0022] Application of the Moringa seed polysaccharide freeze-dried powder of the present invention in cosmetics.

[0023] The application of the present invention, wherein the cosmetic is one of the following:

[0024] (1) Moringa seed polysaccharide essence water is prepared from the following raw materials in parts by weight:

[0025] 1,3-Butanediol 2-3%, ammonium acryloyldimethyltaurate / VP copolymer 0.1-0.5%, carbomer 0.05-0.1%, betaine 0.2-0.5%, p-hydroxyacetophenone 0.3-0.5%, Moringa seed polysaccharide 0.1-0.15%, 1,2-hexanediol 0.3-0.5%, triethanolamine 0.1-0.3%, the balance is water;

[0026] (3) Moringa seed polysaccharide essence milk, prepared from the following raw materials in parts by weight:

[0027] Acrylates / C10-30 Alkyl Acrylate Crosspolymer 0.1-0.15%, Xanthan Gum 0.05-0.1%, 1,3-Butanediol 3-5%, Dipropylene Glycol 3-5%, Glycerin 2-3%, Sodium Hyaluronate 0.01-0.02%, Moringa Seed Polysaccharide Freeze-dried Powder 0.1-0.12%, Polydimethylsiloxane 2-5%, Squalane 1-2%, 9% KOH 0.3-0.5%, Phenoxyethanol 0.5-0.8%, the balance is water;

[0028] (2) Moringa seed polysaccharide essence cream is made from the following raw materials in parts by weight:

[0029] Sodium hyaluronate 0.01-0.05%, Moringa seed polysaccharide freeze-dried powder 0.05-0.1%, xanthan gum 0.1-0.3%, EDTA-Na 0.05-0.1%, 1,3-butylene glycol 2-3%, glycerol 2-5%, cetearyl alcohol 0.5-1%, squalane 2-5%, squalane 1-3%, caprylic / capric triglyceride 2-3%, p-hydroxyacetophenone 0.5-0.8%, 1,2-hexanediol 0.3-0.6%, Seppic 305 (polyacrylamide, C13-14 isoparaffin, laureth-7) 0.8-1%, and the balance is water.

[0030] The differences between the present invention and the prior art, the technical problems addressed and the processing methods (beneficiary effects):

[0031] First, the current utilization rate of Moringa seeds is relatively low. Moringa seed meal after oil extraction is mostly directly processed as animal feed. The various active substances such as protein, polysaccharide, polyphenol, etc. in Moringa seeds are not fully utilized, and farmers' income is relatively low. The present invention uses a natural low eutectic solvent to extract Moringa seed polysaccharides from Moringa seed meal, improves the application efficiency of Moringa seeds, and enhances the deep processing degree of related industries. The extraction solvents used are all raw materials included in the "List of Used Cosmetic Raw Materials", which guarantees the safety of the raw materials to the greatest extent, avoids contact with toxic and harmful reagents, and ensures the safety of consumers.

[0032] Second, a set of corresponding filtering devices is designed for the process of extracting Moringa seed polysaccharides with natural low eutectic solvents. Since Moringa seed meal has a high viscosity after extraction, the filtering efficiency of Moringa seed polysaccharide extract is greatly reduced, which wastes manpower and material resources and increases production costs. The present invention is matched with the designed filtering device to filter it in stages, which improves the filtering effect to a certain extent and saves time and cost.

[0033] Third, the Moringa polysaccharide is freeze-dried to remove moisture from the Moringa seed polysaccharide under a low temperature environment, thereby extending the shelf life of the Moringa seed polysaccharide and saving storage area without preservation. Fourth, based on the extraction of Moringa seed polysaccharide, the feasibility and stability of its application in cosmetic formulas are verified, providing support for the application of Moringa seed polysaccharide extracted by natural low eutectic solvent in cosmetics.

[0034] As a substitute for traditional solvents, natural low eutectic solvents are low eutectic mixtures composed of (HBA) hydrogen bond acceptors or (HBD) hydrogen bond donors in natural substances. They are a new type of solvent. Compared with traditional extraction solvents, natural low eutectic solvents have higher solubility and extraction effect, relatively low melting point, easy access to required materials, simple synthesis methods, low toxicity, high environmental protection, good recyclability, and good biodegradability. In summary, natural low eutectic solvents composed of natural compounds in the "List of Used Cosmetic Raw Materials" such as sugars, organic acids, and polyols are more suitable for the extraction of cosmetic raw materials with higher requirements for safety and efficacy, and effectively avoid contact with toxic or non-biodegradable organic solvents.

[0035] The present invention extracts Moringa seed polysaccharides from the Moringa seed meal remaining after supercritical fluid technology extracts oil through a natural low eutectic solvent, and uses betaine as a hydrogen bond acceptor and propylene glycol as a hydrogen bond donor, and configures them in a molar ratio of 1:1. The extracted Moringa seed polysaccharides are concentrated under reduced pressure, allowed to stand by alcohol precipitation, and washed with anhydrous ethanol for multiple times, and the separated Moringa seed polysaccharides are freeze-dried under the conditions of 0.37 bar and -80 degrees Celsius. Moringa seed polysaccharides are applied in cosmetic formulas in a certain proportion, and the moisturizing effect and safety of Moringa seed polysaccharides are verified by weighing moisturizing tests, cell tests, human efficacy tests, etc., to obtain products with moisturizing effects, and provide support for application in cosmetics. Moringa seed polysaccharides, as a plant-derived raw material extracted by a safe and effective green process, can be widely used in cosmetic products and can achieve good moisturizing effects. Extracting plant polysaccharides from the by-products of Moringa seed oil has far-reaching significance for developing Moringa seed resources, improving the economic added value of related industries, promoting farmers' income, and expanding the application range of Moringa seed raw materials.

[0036] Currently, moringa seed meal is mostly used as animal feed, which has relatively low economic value and insufficient resource utilization. The present invention combines natural low eutectic solvent extraction technology with freeze-drying technology to optimize the extraction process of moringa seed meal raw materials, and cooperates with corresponding filtering devices to improve the extraction and separation efficiency of moringa seed polysaccharides. While promoting the in-depth development of the moringa seed industry, it also provides new ideas for cosmetic raw materials.

[0037] The following is a further description of the extraction device, extraction method and application of the freeze-dried Moringa seed polysaccharide of the present invention in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a schematic diagram of the longitudinal cross-sectional structure of the filtering device for extracting freeze-dried Moringa seed polysaccharides according to the present invention;

[0039] Figure 2 The present invention is a flow chart of the method for extracting freeze-dried Moringa seed polysaccharides. DETAILED DESCRIPTION

[0040] 1. Filtration device for extracting freeze-dried Moringa seed polysaccharides

[0041] like Figure 1 As shown, the filtering device for extracting freeze-dried Moringa seed polysaccharides has five layers of filtering structures fixed in sequence from top to bottom: a first filter screen 4, a second filter screen 5, a macroporous resin 6, an activated carbon filter layer 7, and a filter paper and ceramic filter layer 8.

[0042] The first filter screen 4 is 80 mesh, the second filter screen 5 is 120 mesh, the macroporous resin 6 is D101 macroporous resin, the thickness of the activated carbon filter layer 7 is 5 mm, the filter paper and ceramic filter sheet layer 8 is a 10ppi ceramic filter sheet, on which a single layer of qualitative filter paper is placed.

[0043] The five-layer filter structure is fixed on the filter layer frame 3 from top to bottom in sequence. A liquid collecting hopper 9 is arranged at the lower part of the filter layer frame 3 and is connected with the filter paper and the ceramic filter sheet layer 8. The filter layer frame 3 and the liquid collecting hopper 9 are fixed inside the filter cylinder wall 2. A liquid collecting funnel 1, a liquid valve 10 and a pressure regulating pipe 11 are respectively arranged on the top, bottom and side of the filter cylinder wall 2; the liquid collecting funnel 1 is connected with the internal space of the filter layer frame 3, the liquid valve 10 is connected with the bottom of the liquid collecting hopper 9, the upper part and the lower part of the pressure regulating pipe 11 are respectively connected with the upper space of the first filter screen 4 and the upper space of the liquid collecting hopper 9, and a pressure regulating valve 12 is arranged on the pressure regulating pipe 11;

[0044] The liquid collecting funnel 1, the filter cylinder wall 2, the filter layer frame 3, the first filter screen 4, the second filter screen 5, the liquid collecting bucket 9, the liquid valve 10, the pressure regulating pipeline 11 and the pressure regulating valve 12 are all made of 304 stainless steel.

[0045] 2. Extraction method of freeze-dried Moringa seed polysaccharide

[0046] like Figure 2 As shown, the freeze-dried Moringa seed polysaccharide extraction method comprises the following steps:

[0047] 1. Defatted Moringa seeds

[0048] The shelled and crushed Moringa seed kernels are degreased by supercritical carbon dioxide fluid extraction to obtain defatted Moringa seed meal.

[0049] The supercritical carbon dioxide fluid extraction method comprises the following steps:

[0050] The Moringa seeds were crushed and loaded into the extraction kettle of HA221-50-10-C supercritical carbon dioxide extraction device (Nantong Hua'an Supercritical Extraction Co., Ltd., the present invention uses an extraction kettle and two separation kettles of the extraction device, namely the first separation kettle and the second separation kettle), the carbon dioxide switch was turned on, and the pressure and temperature of the extraction kettle and the first separation kettle and the second separation kettle were adjusted. When the extraction pressure reached 35MPa and the extraction temperature reached 40°C, the pressure of the first separation kettle was 12MPa and the separation temperature was 45°C, and the pressure of the second separation kettle was 5MPa and the separation temperature was 35°C, the valve was adjusted to stabilize it; the carbon dioxide fluid and the The moringa seed material in the extraction kettle is fully contacted, and the carbon dioxide fluid carrying moringa seed oil is passed into the first separation kettle through a pipeline to separate the moringa seed oil for the first time, and then a small amount of unseparated moringa seed oil is allowed to enter the second separation kettle with the carbon dioxide fluid for secondary separation, and the separated carbon dioxide is circulated through a loop; when the preset pressure and temperature of the extraction kettle, the first separation kettle and the second separation kettle are stable for a period of time, the moringa seed oil is collected from the first separation kettle and the second separation kettle every 30 minutes; the overall extraction time is 3 hours, and after the extraction is completed, the moringa seed oil is obtained by merging, and the defatted moringa seed meal in the extraction kettle is collected.

[0051] 2. Preparation of natural deep eutectic solvent

[0052] Betaine and 1,3-butanediol were placed in a beaker at a molar ratio of 1:1, heated and stirred at 80°C until a transparent and uniform liquid was formed, and then cooled to room temperature and stored for later use (DESs).

[0053] 3. Moringa seed polysaccharide extraction

[0054] DESs and distilled water were mixed evenly to prepare a DESs solution with a water content of 85%, and polysaccharide was extracted from Moringa seed meal according to the conditions of the solid-liquid ratio (g:mL) of Moringa seed meal: DESs solution in Table 1. The mixed moringa seed meal slurry was graded and filtered through the extraction and filtration device of the present invention to obtain a clarified Moringa seed polysaccharide extract. After reduced pressure concentration and drying (-0.1MPa; 50-60°C), it was concentrated to 1 / 3 of the original volume, and then anhydrous ethanol was added according to the ratio of Moringa seed polysaccharide extract: anhydrous ethanol of 1:3 (mL:mL), and the alcohol precipitation was static for 12 hours. The supernatant was removed by high-speed centrifugation at 10000r / min for 10min, and water was added to dissolve, filtered with a single-layer qualitative filter paper (model: 101; filtration rate: fast), and concentrated under reduced pressure (-0.1MPa; 50-60°C) to 1 / 3 of the original volume, and anhydrous ethanol was added in a ratio of 1:3 (mL:mL) of Moringa seed polysaccharide extract: anhydrous ethanol. The mixture was precipitated with alcohol for 12h and centrifuged to obtain Moringa seed polysaccharide. The extract was repeatedly washed with 95% anhydrous ethanol until the washing liquid was clear, and the Moringa seed polysaccharide was filtered to obtain the Moringa seed polysaccharide.

[0055] Table 1 Extraction conditions of Moringa seed polysaccharides

[0056] Material-liquid ratio (g:mL) Extraction temperature (℃) Extraction time (min) Polysaccharide yield (%) 1:5 70 120 24.5％ 1:20 70 120 3.6％ 1:30 70 120 3.2％ 1:50 70 120 2.3％

[0057] Note: After the conditions in Table 1 were optimized, the final results showed that the polysaccharide yield was the highest when the solid-liquid ratio (g:mL) of Moringa seed meal:DESs solution was 1:5.

[0058] 4. Freeze drying of Moringa seed polysaccharide

[0059] The obtained Moringa seed polysaccharide was pre-frozen at -80°C for 12 hours, and freeze-dried for 3-4 hours at -80°C and 0.37 mbar to finally obtain Moringa seed polysaccharide freeze-dried powder.

[0060] 3. Application of Moringa Seed Polysaccharide Freeze-dried Powder

[0061] 1. Moringa seed polysaccharide essence water

[0062] (1) Formulation preparation

[0063] The raw materials are calculated based on 100% total mass, including 2-3% 1,3-butanediol, 0.1-0.5% ammonium acryloyldimethyltaurate / VP copolymer, 0.05-0.1% carbomer, 0.2-0.5% betaine, 0.3-0.5% p-hydroxyacetophenone, 0.1-0.15% moringa seed polysaccharide, 0.3-0.5% 1,2-hexanediol, 0.1-0.3% triethanolamine, and the balance is water.

[0064] The preparation method of the moringa seed polysaccharide essence water comprises the following steps: adding phase A raw materials in sequence, stirring and heating to 80-85°C, and keeping warm for 20 minutes; cooling down after the materials are completely dispersed; cooling down to 75°C, adding phase B raw materials, and stirring evenly; cooling down to below 45°C, adding phase C and phase D raw materials in sequence, and stirring for 5-10 minutes until the materials are evenly dispersed.

[0065] Table 2 Moringa seed polysaccharide essence water formula

[0066]

[0067] (2) Cytotoxicity test

[0068] The cytotoxicity of the samples was evaluated by testing the effect of Moringa seed polysaccharide essence water on the survival rate of HaCaT cells in the stratum corneum of human skin.

[0069] Table 3 Analysis of cytotoxicity test results

[0070] Concentration (%, W / V) Cell survival rate (%) Standard deviation (%) Negative control 100.00 0.01 0.05 99.93 0.02 0.10 99.69 0.02 0.25 99.53 0.01 0.50 99.25 0.04 1.00 91.84 0.02 2.00 86.96 0.04

[0071] Moringa seed polysaccharide extract water had no obvious cytotoxicity to HaCaT cells at a concentration below 2% after 24 hours.

[0072] (3) Human patch test

[0073] Patch test method: Select qualified patch test equipment and use the closed patch test method. Place approximately 0.020g~0.025g (solid) or 0.020mL~0.025mL (liquid) of the test substance in the patch test device and apply it to the subject's forearm with low-allergenic tape. Remove the test substance after 24 hours and observe the skin reaction at 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standards in the current valid technical specifications.

[0074] Table 4 Human skin patch test results

[0075]

[0076] Test results: The results of the human skin patch test showed that 30 subjects had no adverse skin reactions in the test area 0.5h, 24h, and 48h after removing the test patch, indicating that Moringa seed polysaccharide essence water is non-irritating to the human body surface.

[0077] (4) Moisturizing effect evaluation (physical weighing test)

[0078] Evaluation method: Under constant temperature and humidity conditions, weigh the mass difference of the sample before and after placement, find out the loss of sample mass, and calculate the moisturizing effect.

[0079] Table 5 Physical moisturizing weighing test

[0080] Test time point (h) 16 24 40 48 64 72 Moisturizing rate (%) 98.76 98.16 96.15 95.34 93.43 92.16

[0081] Test results: The moisturizing rate of Moringa seed polysaccharide essence water was above 90% at the test times of 16h, 24h, 40h, 48h, 64h and 72h, respectively, indicating that it has moisturizing effect under the test conditions of physical weighing method.

[0082] (5) Human efficacy test

[0083] The data information on the moisture content of the stratum corneum of 31 subjects was collected through the skin stratum corneum moisture content test probe, and the moisturizing effect of Moringa seed polysaccharide essence water was analyzed.

[0084] Table 6 Statistical table of moisture content of cheek skin stratum corneum

[0085]

[0086] Note: Improvement rate after using the product (%) = (test value after using the product for N days - basic value) / basic value × 100%

[0087] Test results: 20 minutes after using Moringa seed polysaccharide essence water, compared with before use, the moisture content of the skin stratum corneum increased by 16.95% (p < 0.05), indicating that the use of Moringa seed polysaccharide essence water has a moisturizing effect.

[0088] (6) Consumer use test

[0089] Experimental method: The subjects should visit the laboratory 14 days (D14) and 28 days (D28) after using Moringa seed polysaccharide essence water, clean the test area with cleaning products, wipe it dry and sit quietly in a constant temperature and humidity room. During the meditation period, fill in the questionnaire based on the subjective feelings after using the sample.

[0090] Table 7 Statistical table of sensory evaluation results of the questionnaire survey of subjects (n=30)

[0091]

[0092] Scoring criteria: 5-strongly agree; 4-agree; 3-neutral; 2-disagree; 1-strongly disagree. Scores > 3 are considered positive options.

[0093] Results of sensory evaluation tests by consumers: After using Moringa Seed Polysaccharide Essence Water, 93.10% of the subjects believed that [the product has a pleasant smell]; 96.67% of the subjects believed that [the product is absorbed quickly when used]; 96.67% of the subjects believed that [the product feels good on the skin]; 93.33% of the subjects believed that [the product is gentle and non-irritating when used]; 96.67% of the subjects believed that [the skin is not sticky after using the product].

[0094] Table 8 Statistics of the efficacy evaluation results of the questionnaire survey of subjects (n=30)

[0095]

[0096] Rating criteria: 5-strongly agree / satisfied; 4-agree / satisfied; 3-average; 2-disagree / dissatisfied; 1-strongly disagree / strongly dissatisfied. Ratings > 3 are considered positive options.

[0097] Results of the efficacy evaluation test by consumers: After using Moringa Seed Polysaccharide Essence Water for 14 days (D14) and 28 days (D28), the results of the efficacy evaluation part of the questionnaire survey showed that 63.33% and 79.31% of the subjects believed that [skin sensitivity and redness were improved]; 70.00% and 86.67% of the subjects believed that [skin barrier ability was improved]; 76.67% and 83.33% of the subjects believed that [skin elasticity was improved]; 73.33% and 86.67% of the subjects believed that [skin elasticity was improved]; .33% and 83.33% of the subjects respectively believed that [dry and fine lines on the skin were improved]; 70.00% and 76.67% of the subjects respectively believed that [dull skin tone was improved]; 80.00% and 90.00% of the subjects respectively believed that [skin hydration was improved]; 80.00% and 86.67% of the subjects respectively believed that [skin felt lastingly moisturized]; 100.00% and 100.00% of the subjects respectively believed that [overall, they were satisfied with the use of the product].

[0098] 2. Moringa Seed Polysaccharide Essence Milk

[0099] (1) Formulation preparation

[0100] The raw materials are calculated based on the total mass of 100%, including 0.1-0.15% of acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.05-0.1% of xanthan gum, 3-5% of 1,3-butylene glycol, 3-5% of dipropylene glycol, 2-3% of glycerol, 0.01-0.02% of sodium hyaluronate, 0.1-0.12% of moringa seed polysaccharide freeze-dried powder, 2-5% of polydimethylsiloxane, 1-2% of squalane, 0.3-0.5% of 9% KOH, 0.5-0.8% of phenoxyethanol, and the balance is water.

[0101] The preparation method of the moringa seed polysaccharide essence milk comprises the following steps: dispersing phase A evenly in water; after pre-dispersing phase B in water, adding phase A, and stirring evenly at room temperature; heating phase A+B and phase C to 75° C. respectively; adding phase C to phase A+B while stirring, and homogenizing with a homogenizer of 4500cp for 10 seconds; lowering the temperature to room temperature, adding KOH, and adjusting the pH to about 6.5; adding phenoxyethanol, and discharging.

[0102] Table 9 Moringa seed polysaccharide essence milk formula

[0103]

[0104] (2) Cytotoxicity test

[0105] The cytotoxicity of the samples was evaluated by testing the effect of Moringa seed polysaccharide essence on the survival rate of HaCaT cells in the stratum corneum of human skin.

[0106] Table 10 Analysis of cytotoxicity test results

[0107] Concentration (%, W / V) Cell survival rate (%) Standard deviation (%) Negative control 100.00 0.02 0.05 97.36 0.01 0.10 91.23 0.04 0.25 90.44 0.01 0.50 89.41 0.03 1.00 85.34 0.02

[0108] Moringa seed polysaccharide extract milk had no obvious cytotoxicity to HaCaT cells at a concentration of 1.00% for 24 hours.

[0109] (3) Human patch test

[0110] Patch test method: Select qualified patch test equipment and use the closed patch test method. Place approximately 0.020g~0.025g (solid) or 0.020mL~0.025mL (liquid) of the test substance in the patch test device and apply it to the subject's forearm with low-allergenic tape. Remove the test substance after 24 hours and observe the skin reaction at 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standards in the current valid technical specifications.

[0111] Table 11 Human skin patch test results

[0112]

[0113] Test results: The results of the human skin patch test showed that 30 subjects had no adverse skin reactions in the test areas 0.5h, 24h, and 48h after removing the test patch, indicating that Moringa seed polysaccharide essence milk is non-irritating to the human body surface.

[0114] (4) Moisturizing effect evaluation (physical weighing test)

[0115] Evaluation method: Under constant temperature and humidity conditions, weigh the mass difference of the sample before and after placement, find out the loss of sample mass, and calculate the moisturizing effect.

[0116] Table 12 Physical moisturizing weighing experiment

[0117] Test time point (h) 16 24 40 48 64 72 Moisturizing rate (%) 98.15 97.56 96.25 95.19 93.54 92.25

[0118] Test results: The moisturizing rate of Moringa seed polysaccharide essence lotion was above 90% at the test times of 16h, 24h, 40h, 48h, 64h and 72h, respectively, indicating that it has moisturizing effect under the test conditions of physical weighing method.

[0119] (5) Human efficacy test

[0120] The data information on the moisture content of the stratum corneum of 31 subjects was collected by using a skin stratum corneum moisture content test probe, and the moisturizing effect of Moringa seed polysaccharide essence lotion was analyzed.

[0121] Table 13 Statistics of moisture content of cheek skin stratum corneum

[0122]

[0123] Note: Improvement rate after using the product (%) = (test value after using the product for N days - basic value) / basic value × 100%

[0124] Test results: 20 minutes after using Moringa polysaccharide essence lotion, the moisture content of the skin stratum corneum increased by 18.47% compared with before use (p < 0.05), indicating that the use of Moringa polysaccharide essence lotion has a moisturizing effect.

[0125] (6) Consumer use test

[0126] Experimental method: The subjects should visit the laboratory 14 days (D14) and 28 days (D28) after using Moringa seed polysaccharide essence milk, clean the test area with cleaning products, dry it and sit quietly in a constant temperature and humidity room. During the meditation period, fill in the questionnaire based on the subjective feelings after using the sample.

[0127] Table 14 Statistical table of sensory evaluation results of the questionnaire survey of subjects (n=30)

[0128]

[0129] Scoring criteria: 5-strongly agree; 4-agree; 3-neutral; 2-disagree; 1-strongly disagree. Scores > 3 are considered positive options.

[0130] Results of consumer sensory evaluation tests: After using the Moringa Seed Polysaccharide Essence Lotion, 96.67% of the subjects believed that [the product has a pleasant smell]; 93.55% of the subjects believed that [the product is absorbed quickly when used]; 93.33% of the subjects believed that [the product feels good on the skin]; 96.67% of the subjects believed that [the product is gentle and non-irritating when used]; 93.33% of the subjects believed that [the skin is not sticky after using the product].

[0131] Table 15 Statistics of the results of the efficacy evaluation part of the questionnaire survey of subjects (n=30)

[0132]

[0133] Rating criteria: 5-strongly agree / satisfied; 4-agree / satisfied; 3-average; 2-disagree / dissatisfied; 1-strongly disagree / strongly dissatisfied. Ratings > 3 are considered positive options.

[0134] Results of the efficacy evaluation test by consumers: After using the Moringa Seed Polysaccharide Essence Lotion for 14 days (D14) and 28 days (D28), the results of the efficacy evaluation part of the questionnaire survey showed that 66.67% and 83.33% of the subjects believed that [skin sensitivity and redness were improved]; 73.33% and 93.10% of the subjects believed that [skin barrier ability was improved]; 73.33% and 90.00% of the subjects believed that [skin elasticity ...3.10% of the subjects believed that [skin barrier ability was improved]; 73.33% and 90.00% of the subjects believed that [skin elasticity was improved]; 73.33% and 93.10% of the subjects believed that [skin elasticity was improved]; 73.33% and 90.00% of the subjects believed that [skin elasticity was improved]; 73.33% and 93.10% of the subjects believed that [skin sensitivity and redness were improved]; 73.33% and 93.10% of the subjects believed that [skin barrier ability was improved]; 73.33% and 90.00% of the subjects believed that [skin elasticity was improved]; 73.33% and 93.10% of the subjects believed that [skin barrier ability was improved]; 73.33% and 93.10% of the subjects believed that [skin elasticity was improved]; 73.33% and 93. .33% and 86.67% of the subjects respectively believed that [dry and fine lines on the skin were improved]; 76.67% and 86.67% of the subjects respectively believed that [dull skin tone was improved]; 83.33% and 96.67% of the subjects respectively believed that [skin hydration was improved]; 80.00% and 93.33% of the subjects respectively believed that [skin felt lastingly moisturized]; 100.00% and 100.00% of the subjects respectively believed that [overall, they were satisfied with the use of the product].

[0135] 3. Moringa Seed Polysaccharide Essence Cream

[0136] (1) Formulation preparation

[0137] The raw materials are calculated based on the total mass of 100%, including 0.01-0.05% sodium hyaluronate, 0.05-0.1% freeze-dried powder of moringa seed polysaccharide, 0.1-0.3% xanthan gum, 0.05-0.1% EDTA-Na, 2-3% 1,3-butylene glycol, 2-5% glycerol, 0.5-1% cetearyl alcohol, 2-5% squalane, 1-3% squalane, 2-3% caprylic / capric triglyceride, 0.5-0.8% p-hydroxyacetophenone, 0.3-0.6% 1,2-hexanediol, 0.8-1% Seppic 305 (polyacrylamide, C13-14 isoparaffin, laureth-7), and the balance is water.

[0138] The preparation method of the moringa seed polysaccharide essence cream comprises the following steps: mixing phase A uniformly, stirring or homogenizing at a low speed to disperse uniformly, and then heating to 70-80°C; mixing phase B and heating to 70-80°C, then adding phase B to phase A, homogenizing, stirring and cooling to 40-45°C; heating and dissolving except Seppic 305 (polyacrylamide, C13-14 isoparaffin, laureth-7) in phase C in advance, cooling to room temperature, adding Seppic 305 (polyacrylamide, C13-14 isoparaffin, laureth-7) and mixing, adding the mixed phase C to phases A and B, homogenizing, cooling to room temperature and then discharging.

[0139] Table 16 Moringa seed polysaccharide essence cream formula

[0140]

[0141] (2) Cytotoxicity test

[0142] The cytotoxicity of the samples was evaluated by testing the effect of Moringa seed polysaccharide essence cream on the survival rate of HaCaT cells in the stratum corneum of human skin.

[0143] Table 17 Cytotoxicity test results analysis

[0144] Concentration (%, W / V) Cell survival rate (%) Standard deviation (%) Negative control 100.00 0.02 0.05 96.51 0.02 0.10 90.25 0.04 0.25 89.15 0.02 0.50 85.21 0.03

[0145] Moringa seed polysaccharide essence cream had no obvious cytotoxicity to HaCaT cells at a concentration of 0.5% after 24 hours of action.

[0146] (3) Human patch test

[0147] Patch test method: Select qualified patch test equipment and use the closed patch test method. Place approximately 0.020g~0.025g (solid) or 0.020mL~0.025mL (liquid) of the test substance in the patch test device and apply it to the subject's forearm with low-allergenic tape. Remove the test substance after 24 hours and observe the skin reaction at 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standards in the current valid technical specifications.

[0148] Table 18 Human skin patch test results

[0149]

[0150] Test results: The results of the human skin patch test showed that 29 subjects had no adverse skin reactions in the test area 0.5h, 24h, and 48h after removing the test patch, indicating that Moringa seed polysaccharide essence cream is non-irritating to the human body surface.

[0151] (4) Moisturizing effect evaluation (physical weighing test)

[0152] Evaluation method: Under constant temperature and humidity conditions, weigh the mass difference of the sample before and after placement, find out the loss of sample mass, and calculate the moisturizing effect.

[0153] Table 19 Physical moisturizing weighing experiment

[0154] Test time point (h) 16 24 40 48 64 72 Moisturizing rate (%) 98.75 97.63 96.27 95.55 93.12 91.56

[0155] Test results: The moisturizing rate of Moringa seed polysaccharide essence cream was above 90% at the test times of 16h, 24h, 40h, 48h, 64h and 72h, respectively, indicating that it has moisturizing effect under the test conditions of physical weighing method.

[0156] (5) Human efficacy test

[0157] The data information on the moisture content of the stratum corneum of 31 subjects was collected by using a skin stratum corneum moisture content test probe, and the moisturizing effect of the Moringa seed polysaccharide essence cream was analyzed.

[0158] Table 20 Statistics of moisture content of cheek skin stratum corneum

[0159]

[0160] Note: Improvement rate after using the product (%) = (test value after using the product for N days - basic value) / basic value × 100%

[0161] Test results: 20 minutes after using Moringa seed polysaccharide essence cream, compared with before use, the moisture content of the skin stratum corneum increased by 32.12% (p < 0.05), indicating that the use of Moringa seed polysaccharide essence cream has a moisturizing effect.

[0162] (6) Consumer use test

[0163] Experimental method: The subjects should visit the laboratory 14 days (D14) and 28 days (D28) after using the Moringa seed polysaccharide essence cream, clean the test area with a cleaning product, dry it and sit quietly in a constant temperature and humidity room. During the meditation period, fill in the questionnaire based on the subjective feelings after using the sample.

[0164] Table 21 Statistical table of sensory evaluation results of the questionnaire survey of subjects (n=30)

[0165]

[0166] Scoring criteria: 5-strongly agree; 4-agree; 3-neutral; 2-disagree; 1-strongly disagree. Scores > 3 are considered positive options.

[0167] Results of consumer sensory evaluation tests: After using the Moringa Seed Polysaccharide Essence Cream, 93.33% of the subjects believed that [the product has a pleasant smell]; 96.67% of the subjects believed that [the product is absorbed quickly when used]; 96.67% of the subjects believed that [the product feels good on the skin]; 93.33% of the subjects believed that [the product is gentle and non-irritating when used]; 90.00% of the subjects believed that [the skin is not sticky after using the product].

[0168] Table 22 Statistics of the efficacy evaluation results of the questionnaire survey of subjects (n=30)

[0169]

[0170] Rating criteria: 5-strongly agree / satisfied; 4-agree / satisfied; 3-average; 2-disagree / dissatisfied; 1-strongly disagree / strongly dissatisfied. Ratings > 3 are considered positive options.

[0171] Results of the efficacy evaluation test by consumers: After using the Moringa Seed Polysaccharide Essence Cream for 14 days (D14) and 28 days (D28), the results of the efficacy evaluation part of the questionnaire survey showed that 65.52% and 80.00% of the subjects believed that [skin sensitivity and redness were improved]; 73.33% and 90.00% of the subjects believed that [skin barrier ability was improved]; 73.33% and 96.67% of the subjects believed that [skin elasticity was improved]; 76 .67% and 83.33% of the subjects respectively believed that [dry and fine lines on the skin were improved]; 73.33% and 90.00% of the subjects respectively believed that [dull skin tone was improved]; 86.67% and 100.00% of the subjects respectively believed that [skin hydration was improved]; 83.33% and 96.67% of the subjects respectively believed that [skin felt lastingly moisturized]; 100.00% and 100.00% of the subjects respectively believed that [overall, they were satisfied with the use of the product].

[0172] The embodiments described above are merely descriptions of preferred implementation modes of the present invention and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A filtering device for extracting freeze-dried Moringa seed polysaccharides, characterized in that: Five layers of filtering structures are fixed in sequence from top to bottom inside the filter: a first filtering screen (4), a second filtering screen (5), a macroporous resin (6), an activated carbon filtering layer (7), and a filter paper and ceramic filtering sheet layer (8).

2. The filtration device for extracting freeze-dried Moringa seed polysaccharides according to claim 1, characterized in that: The first filter screen (4) is 80 mesh, the second filter screen (5) is 120 mesh, the macroporous resin (6) is D101 macroporous resin, the thickness of the activated carbon filter layer (7) is 5 mm, the filter paper and ceramic filter sheet layer (8) is a 10ppi ceramic filter sheet, on which a single layer of qualitative filter paper is arranged.

3. The filtration device for extracting freeze-dried Moringa seed polysaccharides according to claim 2, characterized in that: The five-layer filter structure is fixed on the filter layer frame (3) in sequence from top to bottom. A liquid collecting hopper (9) is provided at the bottom of the filter layer frame (3) and is connected to the filter paper and the ceramic filter sheet layer (8). The filter layer frame (3) and the liquid collecting hopper (9) are fixed inside the filter cylinder wall (2). A liquid collecting funnel (1), a liquid valve (10) and a pressure regulating pipe (11) are respectively provided on the top, bottom and side of the filter cylinder wall (2); the liquid collecting funnel (1) is connected to the internal space of the filter layer frame (3), the liquid valve (10) is connected to the bottom of the liquid collecting hopper (9), the upper part and the lower part of the pressure regulating pipe (11) are respectively connected to the upper space of the first filter screen (4) and the upper space of the liquid collecting hopper (9), and a pressure regulating valve (12) is provided on the pressure regulating pipe (11); The liquid collecting funnel (1), the filter cylinder wall (2), the filter layer frame (3), the first filter screen (4), the second filter screen (5), the liquid collecting hopper (9), the liquid valve (10), the pressure regulating pipe (11) and the pressure regulating valve (12) are all made of 304 stainless steel.

4. A method for extracting freeze-dried Moringa seed polysaccharides, characterized in that: The steps include: (1) degreasing the shelled and crushed Moringa seed kernels by supercritical carbon dioxide fluid extraction to obtain defatted Moringa seed meal; (2) natural deep eutectic solvents, i.e., DESs, were prepared using betaine and 1,3-butanediol; (3) Extraction of Moringa Seed Polysaccharides: Prepare a DESs solution, extract polysaccharides from Moringa seed meal, obtain a mixed slurry of Moringa seed meal, and grade filter the mixed slurry of Moringa seed meal using the filtering device described in claim 1 to obtain a clarified Moringa seed polysaccharide extract, and obtain Moringa seed polysaccharides after decompression, concentration, drying, washing and filtering twice; (4) freeze-drying the Moringa seed polysaccharide to obtain the final product, Moringa seed polysaccharide freeze-dried powder.

5. The method for extracting freeze-dried Moringa seed polysaccharides according to claim 4, characterized in that: The supercritical carbon dioxide fluid extraction method comprises the following steps: The moringa seeds are crushed and loaded into the extraction kettle, the carbon dioxide switch is turned on, and the pressure and temperature of the extraction kettle, the first separation kettle and the second separation kettle are adjusted. When the extraction pressure is 35MPa and the extraction temperature is 40°C, the pressure of the first separation kettle is 12MPa and the separation temperature is 45°C, and the pressure of the second separation kettle is 5MPa and the separation temperature is 35°C, the valve is adjusted to stabilize; the carbon dioxide fluid is fully in contact with the moringa seed material in the extraction kettle, and the carbon dioxide fluid carrying moringa seed oil is passed into the first separation kettle through a pipeline to separate the moringa seed oil for the first time, and then a small amount of unseparated moringa seed oil is allowed to enter the second separation kettle with the carbon dioxide fluid for secondary separation, and the separated carbon dioxide is circulated through a loop; when the preset pressure and temperature of the extraction kettle, the first separation kettle and the second separation kettle are stable for a period of time, the moringa seed oil is collected from the first separation kettle and the second separation kettle every 30 minutes; the overall extraction time is 3h, and after the extraction is completed, the moringa seed oil is obtained by merging, and the defatted moringa seed meal in the extraction kettle is collected.

6. The method for extracting freeze-dried Moringa seed polysaccharides according to claim 5, characterized in that: Step (2) specifically comprises the following steps: betaine and 1,3-butanediol are placed in a beaker at a molar ratio of 1:1, heated and stirred at 80° C. until a transparent and uniform liquid, a natural low eutectic solvent, i.e., DESs, is formed, and cooled to room temperature for storage.

7. The method for extracting freeze-dried Moringa seed polysaccharides according to claim 6, characterized in that: Step (3) specifically comprises the following steps: uniformly mixing DESs with distilled water to prepare a DESs solution with a water content of 85%, extracting polysaccharides from the Moringa seed meal at a solid-liquid ratio of Moringa seed meal to DESs solution of 1:5, at an extraction temperature of 70° C. for 120 min, to obtain a mixed slurry of Moringa seed meal; The mixed slurry of Moringa seed meal is graded and filtered through a filtering device to obtain a clarified Moringa seed polysaccharide extract, which is concentrated to 1 / 3 of the original volume by vacuum concentration and drying at a pressure of -0.1 MPa and a temperature of 50-60°C, and then anhydrous ethanol is added in a ratio of 1:3 between the volume of Moringa seed polysaccharide extract and anhydrous ethanol, and the mixture is precipitated with alcohol for 12 hours, and the supernatant is removed by high-speed centrifugation at 10000 r / min for 10 minutes, and water is added to dissolve the mixture, and the mixture is filtered with a single layer of qualitative filter paper, and vacuum concentration is performed at a pressure of -0.1 MPa and a temperature of 50-60°C, and the mixture is concentrated to 1 / 3 of the original volume, and anhydrous ethanol is added in a ratio of 1:3 between the volume of Moringa seed polysaccharide extract and anhydrous ethanol, and the mixture is precipitated with alcohol for 12 hours, and the Moringa seed polysaccharide is obtained by centrifugation, and the mixture is repeatedly washed with 95% anhydrous ethanol until the washing liquid is clarified, and the Moringa seed polysaccharide is obtained by filtration.

8. The method for extracting freeze-dried Moringa seed polysaccharides according to claim 7, characterized in that: Step (4) specifically includes the following steps: pre-freezing the obtained Moringa seed polysaccharide at -80°C for 12 hours, freeze-drying for 3-4 hours at -80°C and 0.37 mbar, and finally obtaining Moringa seed polysaccharide freeze-dried powder.

9. Use of the Moringa seed polysaccharide freeze-dried powder according to any one of claims 4 to 8 in cosmetics.

10. The use according to claim 9, characterized in that: The cosmetic is one of the following: (1) Moringa seed polysaccharide essence water is prepared from the following raw materials in parts by weight: 1,3-Butanediol 2-3%, ammonium acryloyldimethyltaurate / VP copolymer 0.1-0.5%, carbomer 0.05-0.1%, betaine 0.2-0.5%, p-hydroxyacetophenone 0.3-0.5%, Moringa seed polysaccharide 0.1-0.15%, 1,2-hexanediol 0.3-0.5%, triethanolamine 0.1-0.3%, the balance is water; (3) Moringa seed polysaccharide essence milk, prepared from the following raw materials in parts by weight: Acrylates / C10-30 Alkyl Acrylate Crosspolymer 0.1-0.15%, Xanthan Gum 0.05-0.1%, 1,3-Butanediol 3-5%, Dipropylene Glycol 3-5%, Glycerin 2-3%, Sodium Hyaluronate 0.01-0.02%, Moringa Seed Polysaccharide Freeze-dried Powder 0.1-0.12%, Polydimethylsiloxane 2-5%, Squalane 1-2%, 9% KOH 0.3-0.5%, Phenoxyethanol 0.5-0.8%, the balance is water; (2) Moringa seed polysaccharide essence cream is made from the following raw materials in parts by weight: Sodium hyaluronate 0.01-0.05%, Moringa seed polysaccharide freeze-dried powder 0.05-0.1%, xanthan gum 0.1-0.3%, EDTA-Na 0.05-0.1%, 1,3-butylene glycol 2-3%, glycerol 2-5%, cetearyl alcohol 0.5-1%, squalane 2-5%, squalane 1-3%, caprylic / capric triglyceride 2-3%, p-hydroxyacetophenone 0.5-0.8%, 1,2-hexanediol 0.3-0.6%, Seppic 305 (polyacrylamide, C13-14 isoparaffin, laureth-7) 0.8-1%, and the balance is water.

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