Bacterial liquid propagation reaction device and method

Abstract

The invention relates to the technical field of bacterial liquid expanding culture, in particular to a bacterial liquid expanding culture reaction device which comprises an expanding culture tank as well as a material homogenizing mechanism, a material transferring mechanism and a filter pressing mechanism which are arranged in the expanding culture tank, the material transferring mechanism comprises a first liquid pumping assembly and a second liquid pumping assembly, the first liquid pumping assembly is arranged in the expanding culture tank and used for sequentially pumping the upper-layer expanding culture liquid from top to bottom, the second liquid pumping assembly is arranged at the bottom of the expanding culture tank and used for pumping the lower-layer expanding culture liquid, and the expanding culture liquid is pumped in a layered mode according to different height distribution of particle metabolites in the expanding culture liquid; the materials are quickly transferred; according to the invention, filtering methods with different fineness degrees can be adopted, spreading culture liquid is extracted while layering is carried out, the spreading culture liquid can be rapidly transferred, the residence time of the spreading culture liquid is reduced, the spreading culture efficiency of bacterial liquid is improved, and the technical problems of relatively large suction resistance and relatively low material transfer efficiency during spreading culture liquid transfer in the prior art are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of bacterial liquid expansion culture, and in particular to a bacterial liquid expansion culture reaction device and method. Background Art

[0002] With the rapid development of biotechnology, bacterial culture expansion plays an increasingly important role in bioengineering, medicine, food and other fields. Traditional bacterial culture expansion methods usually rely on manual operation, which is not only inefficient but also easily affected by human factors, resulting in unstable expansion results. In order to solve this problem, automated bacterial culture expansion devices came into being.

[0003] The patent document with patent number CN2024203994662 discloses a device for automatic expansion and cultivation of tea wine production strains, including: an expansion tank, an air guide plate, a blower, an air guide pipe, a circulation pipeline, a stirring paddle, a bearing, a motor, an exhaust valve, a CIP cleaning port, a material pipe, a rotary joint, a liquid level sensor, a temperature sensor, a dissolved oxygen sensor, an air pressure sensor, a discharge port, a liquid pump, a three-way joint, a material pipeline, a material pipeline valve, a cleaning pipeline, a cleaning pipeline valve and an air guide pipe valve.

[0004] However, in actual use, the inventors found that after the bacterial liquid had been propagated for a period of time, it needed to be transferred to a larger expansion tank for expansion. However, since the metabolites produced by the bacteria were precipitated to the lower layer of the expansion liquid, the traditional method was to use a pump to pump the bacterial liquid from the bottom of the expansion tank for transfer, but the suction resistance was large and the material transfer efficiency was low. Summary of the invention

[0005] The purpose of the present invention is to address the shortcomings of the prior art. By setting up a bacterial liquid expansion reaction device and method, it is possible to adopt filtering methods of different fineness levels, extract the expansion liquid in layers at the same time, quickly transfer the expansion liquid, reduce the retention time of the expansion liquid, and improve the expansion efficiency of the bacterial liquid, thereby solving the technical problems of large suction resistance and low material transfer efficiency when transferring the expansion liquid.

[0006] In view of the above technical problems, the technical solutions adopted are as follows: A bacterial liquid expansion reaction device comprises an expansion tank, and a material mixing mechanism, a material transfer mechanism and a filter pressing mechanism arranged inside the expansion tank; The material transfer mechanism includes a first liquid extraction component arranged inside the culture expansion tank and used to extract the upper layer culture expansion liquid from top to bottom in sequence, and a second liquid extraction component arranged at the bottom of the culture expansion tank and used to extract the lower layer culture expansion liquid, and the culture expansion liquid is extracted in layers according to the different height distributions of the particle metabolites in the culture expansion liquid to quickly transfer the material; The material mixing mechanism mixes the culture liquid and the bacterial liquid back and forth from top to bottom inside the culture tank, and the particulate metabolites are successively precipitated to the bottom of the culture tank. The first liquid extraction component extracts the upper layer of culture liquid from top to bottom in sequence and quickly collects the filtrate. The second liquid extraction component extracts the lower layer of culture liquid and leaves the particulate metabolites at the bottom of the culture tank. The filter pressing mechanism filters the particulate metabolites at the bottom of the culture tank into a mud cake and cleans out the mud cake.

[0007] Preferably, the expansion culture tank comprises a cover body which is slidably and sealingly arranged on the top of the expansion culture tank, and a liquid inlet pipe, a liquid outlet pipe, a steam inlet pipe and a feeding port which are sequentially arranged on the cover body.

[0008] Preferably, the material mixing mechanism comprises a stirring assembly which is lifted and lowered inside the culture expansion tank, and a lifting assembly which is arranged on the cover and is used to drive the stirring assembly to be lifted and lowered.

[0009] Preferably, the lifting assembly includes a first screw rod rotatably arranged in the middle position of the cover body, a first threaded block threadedly arranged on the first screw rod, a special-shaped bottom plate rotatably arranged at the lower end of the first screw rod, a special-shaped slot opened at the bottom of the expansion tank and matching with the special-shaped bottom plate, and a first driving member arranged on the cover body and used to drive the first screw rod.

[0010] Preferably, the stirring assembly includes a disc arranged on the first threaded block and slidingly matched with the inner wall of the expansion tank, a plurality of groups of strip holes opened on the disc along the circumferential direction, a plurality of groups of first notches opened at the edge of the disc along the circumferential direction and corresponding to the positions of the strip holes, a plurality of groups of limiting strip frames erected on the inner wall of the expansion tank and slidingly matched with the first notches, a shaft rotatably arranged inside the strip holes and one end of which passes through the first notches, a mixing plate arranged on the shaft and located inside the strip holes, a gear arranged at one end of the shaft and located inside the first notch, and a rack arranged on an inner side wall of the limiting strip frame and used to drive the gear.

[0011] Preferably, the first liquid extraction assembly comprises a suction unit disposed on the disc and a collection unit disposed on the disc and used for collecting filtered matter; The suction unit comprises a hose arranged on the disc and one end of which is connected to the liquid outlet pipe, a plurality of groups of first filter holes arranged on the inner side walls of the strip holes and connected to the hose, two groups of cavities symmetrically arranged on the two side walls of the mixing plate, a slide plate slidably arranged inside the cavity, an elastic retractable member arranged between one side of the slide plate and the inner wall of the cavity, two groups of folding protective plates symmetrically arranged between one side of the slide plate and the inner wall of the cavity, a triangular scraper arranged on the other side of the slide plate and used for cleaning the inner side walls of the strip holes, a first valve arranged on the liquid outlet pipe, and a first pump body arranged on the liquid outlet pipe; The collecting unit includes several groups of door frames arranged on the disc and corresponding to the positions of the strip holes respectively, two groups of collecting boxes respectively arranged on the two inner walls of the door frames, a scraper arranged between the upper ports of the two collecting boxes and used to scrape off the filtered material on the triangular scraper strip, a second screw rod rotatably arranged between the two side walls of the door frame, a second threaded block threadedly arranged on the second screw rod, a push plate arranged at the bottom of the second threaded block and used to clean the filtered material on the scraper to the collecting box, a chip removal door opened on the side of the collecting box, a water leakage hole arranged on the chip removal door, and a second driving member arranged on the door frame and used to drive the second screw rod.

[0012] Preferably, the second liquid pumping assembly includes a drain pipe arranged at the bottom of the expansion tank, a second filter hole arranged at the connection between one end of the drain pipe and the expansion tank, a second valve arranged on the drain pipe, a second pump body arranged on the drain pipe, a cleaning wastewater drain pipe arranged at the bottom of the expansion tank, and a third valve arranged on the cleaning wastewater drain pipe.

[0013] Preferably, the filter press mechanism comprises a positioning assembly arranged on the disc and used to limit the mixing plate to maintain a horizontal state, and a lifting assembly arranged outside the expansion tank and used to lift the mud cake; The positioning assembly includes several groups of second notches opened at the edge of the disc and respectively located on both sides of the first notch, an L-shaped clamping plate that passes through and is slidably arranged on the inner side wall of the second notch and extends to the inside of the strip hole, an elastic member arranged between the L-shaped clamping plate and the inner side wall of the second notch, and several groups of limiting rails arranged on the inner wall of the expansion tank and used to drive the L-shaped clamping plate to translate to the inside of the strip hole respectively.

[0014] Preferably, the lifting assembly includes an ear plate arranged on the top of the expansion tank, a third screw rod rotatably arranged between the support leg of the expansion tank and the ear plate, a third threaded block threadedly arranged on the third screw rod, a top rod arranged on the third threaded block and whose upper end passes through the ear plate to drive the cover body to rise and fall, and a third driving member arranged on the support leg of the expansion tank and used to drive the third screw rod.

[0015] Preferably, a bacterial liquid expansion method comprises the following steps: Step 1, feeding process, connecting the expansion tanks of different volumes and specifications in sequence through the connecting pipes, each expansion tank is filled with a corresponding volume of expansion liquid, and then the bacterial liquid cultivated in the laboratory is introduced into a smaller volume expansion tank through the liquid inlet pipe; Step 2: Adjustment process, real-time monitoring of the temperature and pH parameters inside the expansion tank, automatically adding cold and heat sources, as well as pH adjustment reagents into the expansion tank through the feeding port, to ensure that the strains grow in a suitable and stable environment; Step 3, mixing process, the material mixing mechanism slowly mixes the culture solution and the bacterial solution from top to bottom inside the culture tank, and the particle metabolites are successively precipitated to the bottom of the culture tank; Step 4, material transfer process, after the bacterial liquid develops in a smaller volume expansion tank for a certain period of time, the first liquid extraction component extracts the upper layer of expansion liquid from top to bottom to the inside of the next expansion tank, and collects the filtrate, while the second liquid extraction component extracts the lower layer of expansion liquid to the inside of the next expansion tank, and retains the particle metabolites at the bottom of the expansion tank. According to the different concentration distribution of the particle metabolites in the upper and lower layers of the expansion liquid, two filtering methods with different fineness are used to quickly transfer the expansion liquid and reduce the retention time of the expansion liquid; Step 5, filter pressing process, in the process of the first liquid extraction component extracting the upper layer of culture liquid from top to bottom in sequence, after the disc descends to the specified position in the culture tank, the positioning component limits the mixing plate to keep it horizontal, the first liquid extraction component stops working, and the disc continues to descend to filter the particulate metabolites at the bottom of the culture tank into a mud cake, and then the lifting component drives the cover body to take out the mud cake for cleaning through the special-shaped bottom plate at the lower end of the first screw rod; Step six, cleaning and sterilization process, clean water is passed from the liquid inlet pipe into the expansion tank for cleaning. After the waste water is discharged from the cleaning waste water discharge pipe, steam is automatically passed from the steam inlet pipe into the expansion tank. The steam is passed into each expansion tank through the connecting pipe for simultaneous sterilization. The automatic control system controls the sterilization temperature to be maintained at 121℃-124℃ through the temperature sensor, and controls the sterilization pressure to be maintained at 0.1MPa through the pressure sensor. The safety valve releases the pressure in case of overpressure, and the sterilization time is 25min-35min.

[0016] Beneficial effects of the present invention: (1) In the present invention, the material mixing mechanism and the material transfer mechanism are arranged to cooperate. On the one hand, according to the different height distributions of the particulate metabolites in the culture solution, filtering methods with different fineness are adopted to extract the culture solution in layers at the same time, so that the culture solution can be quickly transferred, the retention time of the culture solution is reduced, and the culture efficiency of the bacterial solution is improved; in the process of sucking the upper layer of culture solution for transfer, the first liquid extraction component extracts the upper layer of culture solution from top to bottom in sequence, and the first liquid extraction component follows the liquid level of the culture solution to drop, so as to ensure that the supernatant of the culture solution is always extracted, thereby reducing the blockage of the filtrate and further achieving the effect of rapid extraction; (2) The present invention cooperates with the set material mixing mechanism and the suction unit. On the one hand, the upper and middle layer culture liquids can be stirred to ensure that the bacterial liquid and the culture liquid are fully mixed, and the stirring work is completed during the lifting process. The culture liquid can be mixed back and forth at different heights in turn, thereby improving the uniform distribution of the bacterial liquid at different heights of the culture liquid and improving the reproduction efficiency of the strains. On the other hand, in the process of extracting the upper layer culture liquid, the filtered material can be cleaned in time to prevent the suction unit from being blocked, thereby increasing the smoothness of the suction unit and improving the working efficiency of the suction unit. (3) In the present invention, by cooperating with the positioning assembly and the lifting assembly, after the material transfer is completed, the particulate metabolites at the bottom of the expansion tank can be filtered into a mud cake, and more expansion liquid can be transferred out, which is also convenient for processing the particulate metabolites and reducing the discharge of pollutants. On the other hand, the mud cake can be automatically taken out, which is convenient for cleaning the mud cake, reducing the residual particulate metabolites inside the expansion tank, and facilitating the internal cleaning of the expansion tank. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0018] Figure 1 The figure is a schematic diagram of the structure of a bacterial liquid expansion reaction device.

[0019] Figure 2 It is a schematic diagram of the structure of the expansion tank.

[0020] Figure 3 This is a schematic diagram of the internal structure of the expansion tank.

[0021] Figure 4 It is a structural diagram of the material mixing mechanism.

[0022] Figure 5 It is a structural schematic diagram of the material transfer mechanism.

[0023] Figure 6It is a schematic diagram of the structure of the stirring component.

[0024] Figure 7 for Figure 6 Schematic diagram of the structure from an upward perspective.

[0025] Figure 8 It is a structural schematic diagram of the card position component.

[0026] Fig. 9 Schematic diagram of the structure of the collection unit.

[0027] Fig.10 It is a schematic diagram of the structure of the suction unit.

[0028] Fig.11 Schematic diagram of the structure inside the cavity.

[0029] Fig.12 It is a schematic diagram of the structure of the limiting track.

[0030] Fig.13 The figure is a schematic diagram of a process for bacterial liquid expansion. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention are clearly and completely described below in conjunction with the accompanying drawings.

[0032] Embodiment 1 like Figure 1-Figure 12 As shown, a bacterial liquid expansion reaction device comprises an expansion tank 1, and a material mixing mechanism 2, a material transfer mechanism 3, and a filter pressing mechanism 4 arranged inside the expansion tank 1; The material transfer mechanism 3 includes a first liquid extraction component 31 disposed inside the culture expansion tank 1 and used to extract the upper layer culture expansion liquid from top to bottom in sequence, and a second liquid extraction component 32 disposed at the bottom of the culture expansion tank 1 and used to extract the lower layer culture expansion liquid. According to the different height distributions of the particle metabolites in the culture expansion liquid, filtering methods of different fineness are adopted to extract the culture expansion liquid in layers and transfer the materials quickly; The mixing mechanism 2 mixes the culture liquid and the bacterial liquid back and forth from top to bottom inside the culture tank 1, and the particulate metabolites are successively precipitated to the bottom of the culture tank 1. The first liquid extraction component 31 extracts the upper layer of culture liquid from top to bottom in sequence and quickly collects the filtrate. The second liquid extraction component 32 extracts the lower layer of culture liquid and leaves the particulate metabolites at the bottom of the culture tank 1. The filter pressing mechanism 4 filters the particulate metabolites at the bottom of the culture tank 1 into a mud cake and cleans out the mud cake.

[0033] It should be noted that the expansion tanks 1 of different volume specifications are connected in sequence through connecting pipes, and each expansion tank 1 is filled with a corresponding volume of expansion liquid. The bacterial liquid cultivated in the laboratory is introduced into a smaller volume expansion tank 1 through the liquid inlet pipe 12, and after a period of reproduction, it is transferred to a larger volume expansion tank 1 for expansion.

[0034] It is worth mentioning that the present invention also includes an automated control system, which realizes full automated control of the bacterial liquid expansion process through the coordinated work of sensors, actuators and control software. Sensors that can monitor temperature, humidity, pH value and pressure in real time are arranged inside the expansion tank 1. These sensors transmit the collected data to the control software. The actuator performs various operations according to the instructions of the control software, such as adjusting the temperature, controlling the stirring speed, adding nutrients, safety valves, etc., so as to make real-time adjustments. The precise control of the actuator system ensures the stability and consistency of the expansion process.

[0035] It is also worth mentioning that the present invention filters the bacterial liquid after the expansion culture, and then adds it to the raw materials of the koji block culture through a pump body and a spray device to strengthen the koji making and improve the fermentation power, liquefaction power and esterification power indicators of the koji block.

[0036] It should also be noted that the metabolites produced by the strains are precipitated to the lower layer of the expansion culture liquid, and the particulate metabolites are less distributed in the upper layer of the expansion culture liquid. The first liquid extraction component 31 with a smaller filter pore can quickly suck the upper layer of the expansion culture liquid for transfer, and the second liquid extraction component 32 with a larger filter pore can continuously suck the lower layer of the expansion culture liquid for transfer.

[0037] In this embodiment, the material leveling mechanism 2 and the material transfer mechanism 3 are arranged in coordination. On the one hand, according to the different height distributions of the particulate metabolites in the culture solution, filtering methods of different fineness are adopted, and the culture solution is extracted in layers at the same time, so that the culture solution can be quickly transferred, the retention time of the culture solution is reduced, and the culture efficiency of the bacterial solution is improved; in the process of pumping the upper culture solution for transfer, the first liquid extraction component 31 extracts the upper culture solution from top to bottom in sequence, and the first liquid extraction component 31 follows the liquid level of the culture solution to drop, ensuring that the supernatant of the culture solution is always extracted, reducing the blockage of the filtrate, and further achieving the effect of rapid extraction.

[0038] In detail, first, the expansion tanks 1 of different volume specifications are connected in sequence through the connecting pipe, and the corresponding volume of expansion liquid is injected into each expansion tank 1, and then the bacterial liquid cultivated in the laboratory is introduced into a smaller volume expansion tank 1 through the liquid inlet pipe 12; then, the temperature, pH value and other parameters inside the expansion tank 1 are monitored in real time, and cold and heat sources, as well as reagents for adjusting the pH value are automatically added to the expansion tank 1 through the feeding port 15 to ensure that the bacteria grow in a suitable and stable environment; then, the material mixing mechanism 2 reciprocates and slowly mixes the expansion liquid and the bacterial liquid from top to bottom inside the expansion tank 1, and the particulate metabolites are precipitated to the bottom of the expansion tank 1 in sequence; then, after the bacterial liquid develops in a smaller volume expansion tank 1 for a certain period of time, the first liquid extraction component 31 extracts the upper layer of expansion liquid from top to bottom to the inside of the next expansion tank 1 in sequence, and collects the filtrate, while the second liquid extraction component 32 extracts the lower layer of expansion liquid to the inside of the next expansion tank 1, The particle metabolites are retained at the bottom of the culture tank 1. According to the different concentration distribution of the particle metabolites in the upper and lower layers of the culture liquid, two filtering methods with different fineness are used to quickly transfer the culture liquid and reduce the retention time of the culture liquid. Then, in the process of the first liquid extraction component 31 extracting the upper layer of culture liquid from top to bottom in sequence, after the disc 211 descends to the specified position in the culture tank 1, the positioning component 41 limits the mixing plate 216 to maintain a horizontal state, the first liquid extraction component 31 stops working, and the disc 211 continues to descend to filter the particle metabolites at the bottom of the culture tank 1 into a mud cake. Then, the lifting component 42 drives the cover body 11 to take out the mud cake for cleaning through the special-shaped bottom plate 223 at the lower end of the first screw rod 221. Finally, clean water is introduced into the culture tank 1 from the liquid inlet pipe 12 for cleaning. After the waste water is discharged from the cleaning waste water discharge pipe 325, high-temperature steam is automatically introduced into the culture tank 1 from the steam inlet pipe 14 for sterilization.

[0039] Further, if Figure 1-Figure 8 and Fig.12 As shown, the expansion tank 1 comprises a cover body 11 which is slidably and sealingly arranged on the top of the expansion tank 1, and a liquid inlet pipe 12, a liquid outlet pipe 13, a steam inlet pipe 14, and a feeding port 15 which are sequentially arranged on the cover body 11; It should be noted that a sealing ring is provided at the connection between the cover 11 and the upper port of the expansion tank 1 to improve the sealing performance; The material mixing mechanism 2 includes a stirring assembly 21 which is lifted and lowered inside the expansion tank 1 and a lifting assembly 22 which is arranged on the cover 11 and is used to drive the stirring assembly 21 to be lifted and lowered; The lifting assembly 22 includes a first screw rod 221 rotatably disposed at the middle of the cover body 11, a first threaded block 222 threadedly disposed on the first screw rod 221, a special-shaped bottom plate 223 rotatably disposed at the lower end of the first screw rod 221, a special-shaped slot 224 opened at the bottom of the expansion tank 1 and matched with the special-shaped bottom plate 223, and a first driving member 225 disposed on the cover body 11 and used to drive the first screw rod 221; The stirring assembly 21 includes a disk 211 disposed on the first threaded block 222 and slidingly matched with the inner wall of the expansion tank 1, a plurality of groups of strip holes 212 opened on the disk 211 along the circumferential direction, a plurality of groups of first notches 213 opened at the edge of the disk 211 along the circumferential direction and corresponding to the positions of the strip holes 212, a plurality of groups of limit strip frames 214 vertically disposed on the inner wall of the expansion tank 1 and correspondingly corresponding to the first notches 213 for sliding cooperation, a shaft 215 rotatably disposed inside the strip holes 212 and one end of which passes through the first notches 213, a mixing plate 216 disposed on the shaft 215 and located inside the strip holes 212, a gear 217 disposed at one end of the shaft 215 and located inside the first notch 213, and a rack 218 disposed on an inner side wall of the limit strip frame 214 and used to drive the gear 217.

[0040] It should be noted that the first driving member 225 adopts an existing stepping motor.

[0041] It is worth mentioning that the gear 217 adopts an existing ratchet. When the disc 211 descends, the rack 218 can drive the gear 217 to rotate. When the disc 211 rises, the rack 218 cannot drive the gear 217 to rotate.

[0042] In this embodiment, by cooperating with the set material mixing mechanism 2 and the suction unit 311, on the one hand, the upper and middle layer culture liquids can be stirred to ensure that the bacterial liquid and the culture liquid are fully mixed, and the stirring work is completed during the lifting process, and the culture liquid can be mixed back and forth at different heights in turn, thereby improving the uniform distribution of the bacterial liquid at different heights of the culture liquid and improving the reproduction efficiency of the strains; on the other hand, in the process of extracting the upper layer culture liquid, the filtered material can be cleaned in time to prevent the suction unit 311 from being blocked, thereby increasing the smoothness of the suction unit 311 and improving the working efficiency of the suction unit 311.

[0043] In detail, the first driving member 225 drives the first threaded block 222 to rise and fall through the first screw rod 221, and the first threaded block 222 drives the disc 211 to rise and fall along the limiting frame 214, and the rack 218 inside the limiting frame 214 drives the gear 217 to rotate with the shaft 215, and the shaft 215 rotates with the mixing plate 216, so as to stir the culture liquid at different heights. In the process that the suction unit 311 descends and gradually extracts the culture liquid in the upper layer, the shaft 215 also rotates with the mixing plate 216. In the time period before and after the mixing plate 216 turns to a horizontal state, the triangular scraper 3117 moves along the inner wall of the strip hole 212, forcing the triangular scraper 3117 to drive the slide plate 3114 to move into the cavity 3113 of the mixing plate 216, so that the triangular scraper 3117 can clean the filtered material intercepted on the first filter hole 3112 on the inner wall of the strip hole 212.

[0044] Further, if Figure 3-Figure 12 As shown, the first liquid extraction component 31 includes a suction unit 311 disposed on the disc 211 and a collection unit 312 disposed on the disc 211 and used to collect filtered matter; The suction unit 311 includes a hose 3111 arranged on the disc 211 and one end of which is connected to the liquid outlet pipe 13, a plurality of groups of first filter holes 3112 opened on the two inner walls of the strip hole 212 and connected to the hose 3111, two groups of cavities 3113 symmetrically opened on the two side walls of the mixing plate 216, a slide plate 3114 slidably arranged inside the cavity 3113, an elastic telescopic member 3115 arranged between one side of the slide plate 3114 and the inner wall of the cavity 3113, two groups of folding protective plates 3116 symmetrically arranged between one side of the slide plate 3114 and the inner wall of the cavity 3113, a triangular scraper 3117 arranged on the other side of the slide plate 3114 and used for cleaning the two inner walls of the strip hole 212, a first valve 3118 arranged on the liquid outlet pipe 13, and a first pump body 3119 arranged on the liquid outlet pipe 13.

[0045] It should be noted that, since the first liquid extraction component 31 extracts the upper layer of the culture fluid from top to bottom in sequence, the first liquid extraction component 31 follows the liquid level of the culture fluid to drop, ensuring that the supernatant of the culture fluid is always extracted. Therefore, the aperture of the first filter hole 3112 is smaller than the aperture of the second filter hole 322, so that the upper layer of the culture fluid can be quickly extracted.

[0046] It should also be noted that when the mixing plate 216 is turned to a horizontal state, the first filter hole 3112 is located above the triangular scraper 3117, thereby preventing particulate metabolites at the bottom of the expansion tank 1 from entering the first filter hole 3112 during the filtration process.

[0047] It is worth mentioning that the elastic telescopic member 3115 is composed of an existing spring and a telescopic rod.

[0048] It is also worth mentioning that the folding protection plate 3116 prevents the expansion liquid from entering the cavity 3113 of the mixing plate 216; It should also be noted that, according to the specifications of the slide plate 3114 and the triangular scraper 3117, before the mixing plate 216 turns to the horizontal state, the triangular scraper 3117 just contacts the upper edge of the inner wall of the strip hole 212, ensuring that the triangular scraper 3117 can stick to the inner wall of the strip hole 212 and enter the strip hole 212, so that the mixing plate 216 can rotate continuously and the triangular scraper 3117 can clean the filtered material intercepted by the first filter hole 3112 on the inner wall of the strip hole 212; The collecting unit 312 includes several groups of door frames 3121 arranged on the disc 211 and corresponding to the positions of the strip holes 212 respectively, two groups of collecting boxes 3122 respectively arranged on the two inner walls of the door frames 3121, a scraper 3123 arranged between the two upper ports of the collecting boxes 3122 and used to scrape off the filtered matter on the triangular scraper 3117, a second screw rod 3124 rotatably arranged between the two side walls of the door frame 3121, a second threaded block 3125 threadedly arranged on the second screw rod 3124, a push plate 3126 arranged at the bottom of the second threaded block 3125 and used to clean the filtered matter on the scraper 3123 to the collecting box 3122, a chip removal door 3127 opened on the side of the collecting box 3122, a water leakage hole arranged on the chip removal door 3127, and a second driving member arranged on the door frame 3121 and used to drive the second screw rod 3124.

[0049] It should be noted that according to the height design of the scraper 3123, before the mixing plate 216 turns to the vertical state, the bottom edge of the inclined surface of the triangular scraper strip 3117 just contacts the side of the scraper 3123, which means that the mixing plate 216 can rotate continuously and the scraper 3123 can clean the filtered material on the triangular scraper strip 3117.

[0050] It should also be noted that the second driving member adopts an existing stepping motor.

[0051] In this embodiment, the suction unit 311 and the collection unit 312 cooperate to continuously extract the upper layer of the culture solution from top to bottom, and clean and collect the filtered matter in time.

[0052] In detail, after the strains have been propagated for a period of time, the lifting assembly 22 drives the disc 211 to descend below the liquid level of the upper layer of the culture liquid, and the first pump body 3119 extracts the upper layer of the culture liquid through the hose 3111. The upper layer of the culture liquid flows into the hose 3111 from the first filter hole 3112, and the disc 211 slowly descends along with the liquid level of the culture liquid. During the descent, the rack 218 drives the gear 217 to slowly rotate with the mixing plate 216. When the mixing plate 216 turns to a horizontal state, the triangular scraper 3117 can clean and take away the strip hole 211. When the mixing plate 216 is turned to the vertical state, the scraper 3123 cleans the filtered matter intercepted by the first filter hole 3112 of the inner wall. At the same time, the second driving member drives the second threaded block 3125 to move the push plate 3126 back and forth on the scraper 3123 through the second screw rod 3124, and cleans the filtered matter on the scraper 3123 into the collection box 3122. After the lifting assembly 42 takes out the mud cake through the special-shaped bottom plate 223, the chip discharge door 3127 is opened to clean the filtered matter in the collection box 3122.

[0053] Further, if Figure 1-Figure 3 and Fig.12 As shown, the second liquid pumping assembly 32 includes a drain pipe 321 arranged at the bottom of the expansion tank 1, a second filter hole 322 arranged at the connection between one end of the drain pipe 321 and the expansion tank 1, a second valve 323 arranged on the drain pipe 321, a second pump body 324 arranged on the drain pipe 321, a cleaning wastewater drain pipe 325 arranged at the bottom of the expansion tank 1, and a third valve arranged on the cleaning wastewater drain pipe 325.

[0054] It should be noted that the first valve 3118 and the second valve 323 both adopt existing check valves to prevent the transferred culture liquid from flowing back into the original culture tank 1.

[0055] In this embodiment, the second liquid extraction component 32 is provided to continuously extract the lower layer of culture fluid for transfer.

[0056] In detail, the second pump body 324 continuously extracts the lower layer of culture fluid through the discharge pipe 321, and merges with the upper layer of culture fluid extracted by the first extraction component 31 to flow into the larger volume culture tank 1, and the particulate metabolites are intercepted at the bottom of the original culture tank 1.

[0057] Further, if Figure 2 and Figure 5-Figure 12 As shown, the filter press mechanism 4 includes a positioning assembly 41 disposed on the disc 211 and used to limit the mixing plate 216 to maintain a horizontal state, and a lifting assembly 42 disposed outside the expansion tank 1 and used to lift the mud cake; The positioning assembly 41 includes a plurality of second notches 411 opened at the edge of the disk 211 and respectively located on both sides of the first notch 213, an L-shaped clamping plate 412 penetrating and slidably arranged on the inner side wall of the second notch 411 and extending to the inside of the strip hole 212, an elastic member 413 arranged between the L-shaped clamping plate 412 and the inner side wall of the second notch 411, and a plurality of limiting rails 414 arranged on the inner wall of the expansion tank 1 and used to drive the L-shaped clamping plate 412 to translate to the inside of the strip hole 212 respectively; The lifting assembly 42 includes an ear plate 421 arranged on the top of the expansion tank 1, a third screw rod 422 rotatably arranged between the support leg of the expansion tank 1 and the ear plate 421, a third threaded block 423 threadedly arranged on the third screw rod 422, a top rod 424 arranged on the third threaded block 423 and having an upper end thereof passing through the ear plate 421 to drive the cover body 11 to rise and fall, and a third driving member arranged on the support leg of the expansion tank 1 and used to drive the third screw rod 422.

[0058] It should be noted that the starting end of the lifting portion of the limiting track 414 and the lower terminal end of the rack 218 are in the same horizontal plane, that is, when the mixing plate 216 passes the position of the lower terminal end of the rack 218, it is just in a horizontal state, the disc 211 continues to descend, and the mixing plate 216 will no longer rotate. At the same time, the lifting portion of the limiting track 414 drives the L-shaped clamping plate 412 to clamp the upper surface of the mixing plate 216, thereby preventing the mixing plate 216 from rotating during the filtration process, thereby improving the filtration effect.

[0059] It should also be noted that the third driving component adopts an existing stepping motor.

[0060] In this embodiment, by cooperating with the provided positioning assembly 41 and the lifting assembly 42, after the material transfer is completed, the particulate metabolites at the bottom of the cultivation tank 1 can be filtered into a mud cake, and more cultivation liquid can be transferred out, which is also convenient for processing the particulate metabolites and reducing the discharge of pollutants. On the other hand, the mud cake can be automatically taken out to facilitate the cleaning of the mud cake, reduce the residual particulate metabolites inside the cultivation tank 1, and facilitate the internal cleaning of the cultivation tank 1.

[0061] Specifically, when the disc 211 descends to the position of the lower terminal of the rack 218, the first liquid extraction component 31 stops working, the mixing plate 216 is just in a horizontal state, the disc 211 continues to descend, and the lifting portion of the limiting track 414 drives the L-shaped clamping plate 412 to clamp the upper surface of the mixing plate 216, so that the mixing plate 216 and the triangular scraper 3117 block the strip hole 212 of the disc 211, and the disc 211 continues to descend to filter the particulate metabolites at the bottom of the expansion tank 1, and the second liquid extraction component 32 continues to work until the disc 211 removes the particulate metabolites at the bottom of the expansion tank 1. After the filter press forms a mud cake, the lifting assembly 22 stops working and the lifting assembly 42 starts working. The third driving member drives the third threaded block 423 with the push rod 424 through the third screw rod 422 to lift the cover body 11. The cover body 11 lifts the mud cake through the special-shaped bottom plate 223 at the lower end of the first screw rod 221 until the mud cake rises to the upper port of the expansion tank 1. Then, the first screw rod 221 is manually fixed and the disc 211 is rotated to make the disc 211 rise away from the special-shaped bottom plate 223. The mud cake is then removed and the chip removal door 3127 is opened to clean the filtered material in the collection box 3122.

[0062] Embodiment 2 like Fig.13 As shown, the components identical or corresponding to those in the first embodiment are marked with the corresponding reference numerals in the first embodiment. For the sake of simplicity, only the differences from the first embodiment are described below. The second embodiment differs from the first embodiment in that: A bacterial liquid expansion method comprises the following steps: Step 1, feeding process, connecting the expansion tanks 1 of different volume specifications in sequence through the connecting pipe, a flow meter is provided on the connecting pipe between two adjacent expansion tanks, each expansion tank 1 is filled with a corresponding volume of expansion liquid, and then the bacterial liquid cultivated in the laboratory is introduced into a smaller volume expansion tank 1 through the liquid inlet pipe 12; Step 2, the adjustment process, real-time monitoring of the temperature and pH parameters inside the expansion tank 1, automatically adding cold and heat sources, as well as reagents for adjusting the pH value, into the expansion tank 1 through the feeding port 15 to ensure that the strains grow in a suitable and stable environment; Step 3, mixing process, the material mixing mechanism 2 slowly mixes the culture solution and the bacterial solution from top to bottom in the culture tank 1, and the particle metabolites are successively precipitated to the bottom of the culture tank 1; Step 4, material transfer process, after the bacterial liquid develops in a smaller volume expansion tank 1 for a certain period of time, the first liquid extraction component 31 extracts the upper layer of expansion liquid from top to bottom to the inside of the next expansion tank 1, and collects the filtrate, while the second liquid extraction component 32 extracts the lower layer of expansion liquid to the inside of the next expansion tank 1, and leaves the particle metabolites at the bottom of the expansion tank 1. According to the different concentration distribution of the particle metabolites in the upper and lower layers of the expansion liquid, two filtering methods with different fineness are used, which can quickly transfer the expansion liquid and reduce the retention time of the expansion liquid; Step 5, filter pressing process, in the process of the first liquid extraction component 31 extracting the upper layer of culture liquid from top to bottom in sequence, after the disc 211 descends to the specified position in the culture tank 1, the positioning component 41 limits the mixing plate 216 to maintain a horizontal state, the first liquid extraction component 31 stops working, and the disc 211 continues to descend to filter the particulate metabolites at the bottom of the culture tank 1 into a mud cake, and then the lifting component 42 drives the cover body 11 to take out the mud cake for cleaning through the special-shaped bottom plate 223 at the lower end of the first screw rod 221; Step six, cleaning and sterilization process, clean water is introduced into the expansion tank 1 from the liquid inlet pipe 12 for cleaning. After the waste water is discharged from the cleaning waste water discharge pipe 325, steam is automatically introduced into the expansion tank 1 from the steam inlet pipe 14. The steam is introduced into each expansion tank through the connecting pipe for simultaneous sterilization. The automatic control system controls the sterilization temperature to be maintained at 121°C-124°C through the temperature sensor, and controls the sterilization pressure to be maintained at 0.1MPa through the pressure sensor. The safety valve releases the pressure when overpressure occurs, and the sterilization time is 25min-35min.

[0063] In the description of the present invention, it is necessary to understand that the directions or positional relationships indicated by the terms "front and back", "left and right", etc. are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or component referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the invention.

[0064] Of course, in the present technical solution, those skilled in the art should understand that the term "one" should be understood as "at least one" or "one or more", that is, in one embodiment, the number of an element may be one, while in another embodiment, the number of the element may be multiple, and the term "one" should not be understood as a limitation on the quantity.

[0065] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art under the technical guidance of the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A bacterial liquid expansion reaction device, characterized in that: It comprises an expansion tank, and a material mixing mechanism, a material transfer mechanism, and a filter pressing mechanism arranged inside the expansion tank; The material transfer mechanism includes a first liquid extraction component arranged inside the culture expansion tank and used to extract the upper layer culture expansion liquid from top to bottom in sequence, and a second liquid extraction component arranged at the bottom of the culture expansion tank and used to extract the lower layer culture expansion liquid, and the culture expansion liquid is extracted in layers according to the different height distributions of the particle metabolites in the culture expansion liquid to quickly transfer the material; The material mixing mechanism mixes the culture liquid and the bacterial liquid back and forth from top to bottom inside the culture tank, and the particulate metabolites are successively precipitated to the bottom of the culture tank. The first liquid extraction component extracts the upper layer of culture liquid from top to bottom in sequence and quickly collects the filtrate. The second liquid extraction component extracts the lower layer of culture liquid and leaves the particulate metabolites at the bottom of the culture tank. The filter pressing mechanism filters the particulate metabolites at the bottom of the culture tank into a mud cake and cleans out the mud cake.

2. A bacterial liquid expansion reaction device according to claim 1, characterized in that: The expansion and cultivation tank comprises a cover body which is slidably and sealingly arranged on the top of the expansion and cultivation tank, and a liquid inlet pipe, a liquid outlet pipe, a steam inlet pipe and a feeding port which are sequentially arranged on the cover body.

3. A bacterial liquid expansion reaction device according to claim 2, characterized in that: The material mixing mechanism comprises a stirring assembly which is lifted and lowered inside the expansion tank and a lifting assembly which is arranged on the cover and is used to drive the stirring assembly to lift and lower.

4. A bacterial liquid expansion reaction device according to claim 3, characterized in that: The lifting assembly includes a first screw rod rotatably arranged in the middle position of the cover body, a first threaded block threadedly arranged on the first screw rod, a special-shaped bottom plate rotatably arranged at the lower end of the first screw rod, a special-shaped slot opened at the bottom of the expansion tank and matched with the special-shaped bottom plate, and a first driving member arranged on the cover body and used to drive the first screw rod.

5. A bacterial liquid expansion reaction device according to claim 4, characterized in that: The stirring assembly includes a disc arranged on the first threaded block and slidingly matched with the inner wall of the expansion tank, a plurality of groups of strip holes opened on the disc along the circumferential direction, a plurality of groups of first notches opened at the edge of the disc along the circumferential direction and corresponding to the positions of the strip holes, a plurality of groups of limit strip frames vertically arranged on the inner wall of the expansion tank and slidingly matched with the first notches, a shaft rotatably arranged inside the strip holes and one end of which passes through the first notches, a mixing plate arranged on the shaft and located inside the strip holes, a gear arranged at one end of the shaft and located inside the first notch, and a rack arranged on an inner side wall of the limit strip frame and used to drive the gear.

6. A bacterial liquid expansion reaction device according to claim 5, characterized in that: The first liquid extraction assembly comprises a suction unit disposed on the disc and a collection unit disposed on the disc and used to collect filtered matter; The suction unit comprises a hose arranged on the disc and one end of which is connected to the liquid outlet pipe, a plurality of groups of first filter holes arranged on the inner side walls of the strip holes and connected to the hose, two groups of cavities symmetrically arranged on the two side walls of the mixing plate, a slide plate slidably arranged inside the cavity, an elastic retractable member arranged between one side of the slide plate and the inner wall of the cavity, two groups of folding protective plates symmetrically arranged between one side of the slide plate and the inner wall of the cavity, a triangular scraper arranged on the other side of the slide plate and used for cleaning the inner side walls of the strip holes, a first valve arranged on the liquid outlet pipe, and a first pump body arranged on the liquid outlet pipe; The collecting unit includes several groups of door frames arranged on the disc and corresponding to the positions of the strip holes respectively, two groups of collecting boxes respectively arranged on the two inner walls of the door frames, a scraper arranged between the upper ports of the two collecting boxes and used to scrape off the filtered material on the triangular scraper strip, a second screw rod rotatably arranged between the two side walls of the door frame, a second threaded block threadedly arranged on the second screw rod, a push plate arranged at the bottom of the second threaded block and used to clean the filtered material on the scraper to the collecting box, a chip removal door opened on the side of the collecting box, a water leakage hole arranged on the chip removal door, and a second driving member arranged on the door frame and used to drive the second screw rod.

7. A bacterial liquid expansion reaction device according to claim 6, characterized in that: The second liquid pumping assembly includes a drain pipe arranged at the bottom of the expansion tank, a second filter hole arranged at the connection between one end of the drain pipe and the expansion tank, a second valve arranged on the drain pipe, a second pump body arranged on the drain pipe, a cleaning wastewater drain pipe arranged at the bottom of the expansion tank, and a third valve arranged on the cleaning wastewater drain pipe.

8. A bacterial liquid expansion reaction device according to claim 7, characterized in that: The filter press mechanism includes a positioning assembly arranged on the disc and used to limit the mixing plate to maintain a horizontal state, and a lifting assembly arranged outside the expansion tank and used to lift the mud cake; The positioning assembly includes several groups of second notches opened at the edge of the disc and respectively located on both sides of the first notch, an L-shaped clamping plate that passes through and is slidably arranged on the inner side wall of the second notch and extends to the inside of the strip hole, an elastic member arranged between the L-shaped clamping plate and the inner side wall of the second notch, and several groups of limiting rails arranged on the inner wall of the expansion tank and used to drive the L-shaped clamping plate to translate to the inside of the strip hole respectively.

9. A bacterial liquid expansion reaction device according to claim 8, characterized in that: The lifting assembly includes an ear plate arranged on the top of the expansion tank, a third screw rod rotatably arranged between the support leg of the expansion tank and the ear plate, a third threaded block threadedly arranged on the third screw rod, a top rod arranged on the third threaded block and having an upper end thereof passing through the ear plate to drive the cover body to be lifted and lowered, and a third driving member arranged on the support leg of the expansion tank and used to drive the third screw rod.

10. A bacterial liquid expansion method, applied to a bacterial liquid expansion reaction device as claimed in any one of claims 1 to 9, characterized in that: The following steps are involved: Step 1, feeding process, connecting the expansion tanks of different volumes and specifications in sequence through the connecting pipes, each expansion tank is filled with a corresponding volume of expansion liquid, and then the bacterial liquid cultivated in the laboratory is introduced into a smaller volume expansion tank through the liquid inlet pipe; Step 2: Adjustment process, real-time monitoring of the temperature and pH parameters inside the expansion tank, automatically adding cold and heat sources, as well as pH adjustment reagents into the expansion tank through the feeding port, to ensure that the strains grow in a suitable and stable environment; Step 3, mixing process, the material mixing mechanism slowly mixes the culture solution and the bacterial solution from top to bottom inside the culture tank, and the particle metabolites are successively precipitated to the bottom of the culture tank; Step 4, material transfer process, after the bacterial liquid develops in a smaller volume expansion tank for a certain period of time, the first liquid extraction component extracts the upper layer of expansion liquid from top to bottom to the inside of the next expansion tank, and collects the filtrate, while the second liquid extraction component extracts the lower layer of expansion liquid to the inside of the next expansion tank, and retains the particle metabolites at the bottom of the expansion tank. According to the different concentration distribution of the particle metabolites in the upper and lower layers of the expansion liquid, two filtering methods with different fineness are used to quickly transfer the expansion liquid and reduce the retention time of the expansion liquid; Step 5, filter pressing process, in the process of the first liquid extraction component extracting the upper layer of culture liquid from top to bottom in sequence, after the disc descends to the specified position in the culture tank, the positioning component limits the mixing plate to keep it horizontal, the first liquid extraction component stops working, and the disc continues to descend to filter the particulate metabolites at the bottom of the culture tank into a mud cake, and then the lifting component drives the cover body to take out the mud cake for cleaning through the special-shaped bottom plate at the lower end of the first screw rod; Step six, cleaning and sterilization process, clean water is passed into the expansion tank from the liquid inlet pipe for cleaning. After the waste water is discharged from the cleaning waste water discharge pipe, high-temperature steam is automatically passed into the expansion tank from the steam inlet pipe for sterilization.

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