Stem cell filtering separator with high uniformity

Abstract

The invention discloses a stem cell filtering separator with high uniformity, and relates to the technical field of stem cell filtering separation. According to the stem cell filtering separator with the high uniformity, through the arrangement of the filtering cylinder and the arc-shaped filtering frame, when a stem cell solution decomposed by the mixing and separating box enters the filtering cylinder through the liquid discharging frame, the stem cell solution can uniformly fall into the arc-shaped filtering frame along with the rotation of the arc-shaped filtering frame in the filtering cylinder; according to the stem cell solution filtering device, the contact area of a solution and the arc-shaped filtering frame is effectively increased, the filtering effect is guaranteed while the filtering speed is increased, meanwhile, through cooperation of the assembly frame and the filtering plate frame, the stem cell solution filtering device can reciprocate up and down along with the filtering plate frame to drive the solution to conduct secondary filtering operation, the filtering uniformity of the stem cell solution is guaranteed, and the filtering effect of the device is improved.

Description

Technical Field

[0001] The invention relates to the technical field of stem cell filtration separation, in particular to a stem cell filtration separator with high uniformity. Background Art

[0002] Stem cells refer to primitive cells with the potential for self-renewal and multidirectional differentiation. They are the origin cells of the filter body and can differentiate into one or more cells that constitute human tissues or organs under specific conditions. Self-renewal means that stem cells can maintain the size of the stem cell population through cell division; multidirectional differentiation means that stem cells have plasticity and can differentiate into different tissue cells under different environments to form various complex tissues and organs of the filter body. When used in clinical practice, stem cells are often separated by cell sieves. Referring to the Chinese patent, the announcement number is "CN108060082A" "A stem cell filter separator", which points out that the purpose of separating cells from digested tissue blocks is achieved by shaking, stirring and oscillating. This stem cell separation method has the problems of low separation efficiency, uneven stirring and stem cells being easily contaminated; but the application still has the problems of poor filtration efficiency and uniformity. In response to this, we propose a stem cell filter separator with high uniformity to solve the above problems. Summary of the invention

[0003] In view of the shortcomings of the prior art, the present invention provides a stem cell filtration separator with high uniformity, which solves the problems raised in the above-mentioned background technology.

[0004] To achieve the above objectives, the present invention is implemented through the following technical solutions: a stem cell filtration separator with high uniformity, comprising a filter body, a mixing and separation box installed inside the filter body, a liquid inlet hopper fixed on the top surface of the filter body, a liquid inlet pipe connected between the liquid inlet hopper and the mixing and separation box for conveying the solution, and the mixing and separation box for mixing the solution and decomposing the stem cells;

[0005] A filter cartridge is fixedly installed inside the filter body, and the installation position of the filter cartridge is located below the mixing and separation box. A transmission disc frame is rotatably installed inside the filter cartridge, and an arc filter frame is fixedly installed inside the transmission disc frame. The arc filter frame is used to perform the first filtering operation on the solution;

[0006] The bottom of the mixing and separation box is connected with a drain pipe, the end of which extends to the arc-shaped filter frame, and is used to transport the solution to the inside of the running arc-shaped filter frame;

[0007] An assembly frame is installed below the filter cartridge, and a filter plate frame is inserted inside the assembly frame. The filter plate frame is used to perform a second filtering treatment on the solution. A transmission component is provided at the bottom of the assembly frame to drive the assembly frame and the filter plate frame to reciprocate at the bottom of the filter cartridge to achieve filtering treatment of the solution. A drainage frame is provided below the filter cartridge, and the drainage frame is fixed to the inner bottom of the filter body. The top surface of the drainage frame is provided as an inclined surface for transporting the stem cell solution.

[0008] Preferably, a cleaning assembly is provided inside the arc-shaped filter frame for cleaning impurities remaining inside the arc-shaped filter frame, the cleaning assembly includes an arc-shaped liquid pushing plate, a collecting plate frame is fixedly installed inside the filter cartridge, the arc-shaped liquid pushing plate is fixedly installed at the end of the collecting plate frame, a rectangular groove is provided at one end of the collecting plate frame close to the arc-shaped liquid pushing plate, and a filter mesh frame is fixedly installed at the bottom of the collecting plate frame.

[0009] Preferably, the end of the arc-shaped liquid pushing plate extends to the inside of the arc-shaped filter frame, and the arc-shaped liquid pushing plate and the inner wall of the arc-shaped filter frame are in contact with each other.

[0010] Preferably, a negative pressure device is fixedly installed inside the filter cartridge, and a delivery pipe is connected between the negative pressure device and the inside of the collecting plate frame for extracting the collected impurities.

[0011] Preferably, a sealing door is rotatably connected to the side of the filter body, a control panel is fixedly installed on the outside of the filter body, and a liquid outlet is opened on the side of the filter body, and the liquid outlet is kept flush with the end of the liquid drain frame.

[0012] Preferably, a stirring shaft is rotatably installed inside the mixing and separation box, a plurality of stirring blades are fixedly installed outside the stirring shaft, a servo motor is fixedly installed outside the mixing and separation box, a transmission chain is connected between the output end of the servo motor and the end of the stirring shaft, and an electric butterfly valve is installed inside the discharge pipe.

[0013] Preferably, a driving assembly is provided on the outer side of the filter cartridge for driving the transmission disc frame to rotate inside it, a fixing frame is fixedly sleeved on the outer side of the filter cartridge, and the fixing frame is fixedly installed inside the filter body, the driving assembly includes a driving gear, a driving motor 1 is fixedly installed on the outer side of the filter cartridge, the driving gear is fixedly sleeved on the output end of the driving motor 1, a transmission gear ring is fixedly installed on the top surface of the transmission disc frame, and the driving gear and the transmission gear ring are meshed with each other.

[0014] Preferably, the transmission assembly includes a transmission frame, there are two transmission frames and both are fixed at the bottom of the assembly frame, one end of the assembly frame away from the transmission frame is rotatably connected to the inner wall of the filter body, a transmission shaft is rotatably installed inside the filter body, and a plurality of arc-shaped transmission blocks are fixedly sleeved on the outer side of the transmission shaft, and the arc-shaped transmission blocks are respectively located inside the corresponding transmission frames and are used to lift the transmission frames.

[0015] Preferably, the plurality of arc-shaped transmission blocks are respectively matched with the plurality of transmission frames.

[0016] Preferably, a second driving motor is fixedly installed inside the filter body, and a transmission belt is rotatably connected between the output end of the second driving motor and the transmission shaft.

[0017] The present invention provides a stem cell filtration separator with high uniformity. Compared with the prior art, it has the following beneficial effects:

[0018] (1) The stem cell filter separator with high uniformity is provided with a filter cartridge and an arc filter frame. When the stem cell solution that has been decomposed by the mixing and separation box enters the filter cartridge through the drainage frame, as the arc filter frame rotates inside the filter cartridge, the stem cell solution can fall evenly inside the arc filter frame, effectively increasing the contact area between the solution and the arc filter frame, improving the filtration speed while ensuring the filtration effect. At the same time, through the cooperation of the assembly frame and the filter plate frame, the filter plate frame can reciprocate up and down to drive the solution to perform a secondary filtration operation, thereby ensuring the filtration uniformity of the stem cell solution and improving the filtration effect of the equipment.

[0019] (2) The high uniformity stem cell filter separator has a cleaning component. When the arc filter frame is running to filter the solution, as the arc filter frame rotates, the solution attached to the inner wall of the arc filter frame can be pushed out through the arc liquid pushing plate. On the one hand, it can avoid solution retention. At the same time, during the filtration process, impurities can enter the interior of the collection plate frame along the arc surface of the arc liquid pushing plate, completing the self-cleaning operation of the arc filter frame. On the one hand, it improves the filtering effect of the solution, and at the same time improves the convenience of equipment maintenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0021] Figure 2 This is a schematic diagram of the cross-sectional structure of the filter body of the present invention;

[0022] Figure 3 For the present invention Figure 2 Front view;

[0023] Figure 4 For the present invention Figure 3 The enlarged structural diagram at A in the middle;

[0024] Figure 5 This is a schematic cross-sectional view of the structure of the mixing and separation box of the present invention;

[0025] Figure 6 This is a schematic diagram of the cross-sectional structure of the filter cartridge of the present invention;

[0026] Figure 7 For the present invention Figure 6 The enlarged structural diagram at B in the middle;

[0027] Figure 8 It is a schematic diagram of the cross-sectional structure of the collecting plate frame of the present invention.

[0028] In the figure: 1, filter body; 101, control panel; 2, sealing door; 3, liquid outlet; 4, liquid inlet bucket; 401, liquid inlet pipe; 5, mixing and separation box; 6, stirring shaft; 601, stirring blade; 7, liquid discharge pipe; 701, electric butterfly valve; 8, servo motor; 801, transmission chain; 9, filter cartridge; 901, fixed frame; 10, transmission disc frame; 1001, arc filter frame; 1002, transmission gear ring; 11, drive motor 1; 1101, drive gear; 12, collection plate frame; 13, arc liquid push plate; 14, negative pressure device; 1201, filter screen frame; 15, assembly frame; 1501, filter plate frame; 16, transmission frame; 17, transmission shaft; 1701, arc transmission block; 18, drive motor 2; 1801, transmission belt; 19, liquid discharge frame. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] See also Figure 1-Figure 8 The present invention provides two technical solutions, which specifically include the following embodiments:

[0031] Embodiment 1:

[0032] In an embodiment of the present invention, a stem cell filtration separator with high uniformity includes a filter body 1, a mixing and separation box 5 is installed inside the filter body 1, a liquid inlet hopper 4 is fixed on the top surface of the filter body 1, and a liquid inlet pipe 401 is connected between the liquid inlet hopper 4 and the mixing and separation box 5 for conveying the solution, and the mixing and separation box 5 is used to mix the solution and decompose the stem cells;

[0033] In the embodiment of the present invention, specifically, the solution to be filtered is poured into the liquid inlet bucket 4, so that the solution enters the mixing and separation box 5 through the liquid inlet bucket 4, and the solution is stirred through the mixing and separation box 5 to decompose the stem cells;

[0034] In the embodiment of the present invention, specifically, a filter cartridge 9 is fixedly installed inside the filter body 1, and the installation position of the filter cartridge 9 is located below the mixing and separation box 5. A transmission disc frame 10 is rotatably installed inside the filter cartridge 9, and an arc filter frame 1001 is fixedly installed inside the transmission disc frame 10. The arc filter frame 1001 is used to perform the first filtering operation on the solution;

[0035] In the embodiment of the present invention, a drain pipe 7 is connected to the bottom of the mixing and separation box 5, and the end of the drain pipe 7 extends to the arc-shaped filter frame 1001, which is used to transport the solution to the inside of the running arc-shaped filter frame 1001;

[0036] In the embodiment of the present invention, specifically, the decomposed solution can enter the filter cartridge 9, and the arc filter frame 1001 inside the transmission disc frame 10 completes the first filtration. During the filtration process, as the arc filter frame 1001 rotates with the transmission disc frame 10, the solution entering the arc filter frame 1001 through the drainage pipe 7 slides down along its arc surface, and the impurities in the solution are filtered, thereby ensuring the filtration uniformity of the solution.

[0037] In the embodiment of the present invention, further, an assembly frame 15 is installed below the filter cartridge 9, and a filter plate frame 1501 is inserted into the assembly frame 15, and the filter plate frame 1501 is used to perform a second filtration treatment on the solution. A drainage frame 19 is provided below the filter cartridge 9, and the drainage frame 19 is fixed to the inner bottom of the filter body 1. The top surface of the drainage frame 19 is provided as an inclined surface for transporting the stem cell solution;

[0038] In the embodiment of the present invention, further, a cleaning component is provided inside the arc filter frame 1001 for cleaning the impurities remaining inside the arc filter frame 1001, and the cleaning component includes an arc liquid pushing plate 13, and a collecting plate frame 12 is fixedly installed inside the filter cartridge 9, and the arc liquid pushing plate 13 is fixedly installed at the end of the collecting plate frame 12, and a rectangular groove is provided at one end of the collecting plate frame 12 close to the arc liquid pushing plate 13, and a filter frame 1201 is fixedly installed at the bottom of the collecting plate frame 12. When the arc filter frame 1001 is running to remove the solvent, When filtering the liquid, the arc-shaped liquid pushing plate 13 is fitted with the inner wall of the arc-shaped filter frame 1001. As the arc-shaped filter frame 1001 rotates, the solution attached to the inner wall of the arc-shaped filter frame 1001 can be pushed by the arc-shaped liquid pushing plate 13. On the one hand, it can avoid the retention of the solution. At the same time, during the filtering process, impurities can enter the inside of the collecting plate frame 12 along the arc surface of the arc-shaped liquid pushing plate 13, completing the self-cleaning operation of the arc-shaped filter frame 1001. On the one hand, it improves the filtering effect of the solution, and at the same time improves the convenience of maintenance of the equipment;

[0039] In the embodiment of the present invention, specifically, the end of the arc-shaped liquid-pushing plate 13 extends to the inside of the arc-shaped filter frame 1001, and the arc-shaped liquid-pushing plate 13 and the inner wall of the arc-shaped filter frame 1001 are mutually attached, and the shape of the arc-shaped liquid-pushing plate 13 is arc-shaped. When the solution is pushed, the solution can pass through the arc-shaped filter frame 1001 and be discharged downward, and the filtered impurities can enter the inside of the collecting plate frame 12 along the arc-shaped liquid-pushing plate 13;

[0040] In the embodiment of the present invention, specifically, a negative pressure device 14 is fixedly installed inside the filter cartridge 9, and a conveying pipe is connected between the negative pressure device 14 and the inside of the collecting plate frame 12, which is used to extract the collected impurities. The impurities entering the collecting plate frame 12 can fall into the filter frame 1201. At this time, the negative pressure device 14 is operated to extract the impurities through the conveying pipe, thereby improving the convenience of maintenance;

[0041] In the embodiment of the present invention, specifically, the negative pressure device 14 is an existing negative pressure pump, which will not be described in detail here;

[0042] In the embodiment of the present invention, specifically, a sealing door 2 is rotatably connected to the side of the filter body 1, a control panel 101 is fixedly installed on the outside of the filter body 1, a liquid outlet 3 is opened on the side of the filter body 1, and the liquid outlet 3 is kept flush with the end of the liquid drain frame 19. The control panel 101 is controlled based on an existing PLC control module, and the liquid outlet 3 is used to discharge the stem cell solution;

[0043] In the embodiment of the present invention, specifically, a stirring shaft 6 is rotatably installed inside the mixing and separation box 5, a plurality of stirring blades 601 are fixedly installed outside the mixing and separation box 5, a servo motor 8 is fixedly installed outside the mixing and separation box 5, a transmission chain 801 is connected between the output end of the servo motor 8 and the end of the stirring shaft 6, an electric butterfly valve 701 is installed inside the discharge pipe 7, and the transmission chain 801 is an existing transmission structure, and the transmission operation is realized by mutually adapted sprockets, which are not shown in the figure;

[0044] In the embodiment of the present invention, after the solution enters the mixing and separation box 5, the servo motor 8 is operated to drive the stirring shaft 6 to operate, and the stirring blade 601 is operated to stir the solution to separate the stem cells;

[0045] In the embodiment of the present invention, specifically, a driving assembly is provided on the outer side of the filter cartridge 9 for driving the transmission disc frame 10 to rotate therein, a fixing frame 901 is fixedly sleeved on the outer side of the filter cartridge 9, and the fixing frame 901 is fixedly installed inside the filter body 1, the driving assembly includes a driving gear 1101, a driving motor 11 is fixedly installed on the outer side of the filter cartridge 9, the driving gear 1101 is fixedly sleeved on the output end of the driving motor 11, a driving gear ring 1002 is fixedly installed on the top surface of the transmission disc frame 10, the driving gear 1101 and the driving gear ring 1002 are meshed with each other, as the driving motor 11 runs, the driving gear 1101 and the driving gear ring 1002 are meshed with each other, driving the transmission disc frame 10 to rotate inside the filter cartridge 9, so that the arc filter frame 1001 can rotate inside the filter cartridge 9 along with the driving disc frame 10, so that when the solution is discharged through the drain pipe 7, it can evenly fall on the inner surface of the arc filter frame 1001, thereby ensuring the filtering effect.

[0046] Embodiment 2: Based on embodiment 1, in the embodiment of the present invention, further, a transmission assembly is provided at the bottom of the assembly frame 15, which is used to drive the assembly frame 15 and the filter plate frame 1501 to reciprocate at the bottom of the filter cartridge 9 to achieve filtering treatment of the solution;

[0047] In the embodiment of the present invention, specifically, the transmission assembly includes a transmission frame 16, and there are two transmission frames 16, which are both fixed at the bottom of the assembly frame 15. One end of the assembly frame 15 away from the transmission frame 16 is rotatably connected to the inner wall of the filter body 1. A transmission shaft 17 is rotatably installed inside the filter body 1. A plurality of arc-shaped transmission blocks 1701 are fixedly sleeved on the outer side of the transmission shaft 17. The arc-shaped transmission blocks 1701 are respectively located inside the corresponding transmission frames 16 and are used to lift the transmission frame 16. When the transmission shaft 17 rotates, it can drive the plurality of arc-shaped transmission blocks 1701 fixedly sleeved on the outer side to rotate. As the plurality of arc-shaped transmission blocks 1701 rotate inside the corresponding transmission frames 16, the assembly frame 15 can run up and down along the inner wall of the filter body 1 and make reciprocating motion, so that the filter plate frame 1501 and the solution above can run with the assembly frame 15, thereby ensuring the filtration uniformity and filtration effect of the stem cell solution.

[0048] In the embodiment of the present invention, specifically, the solution that has completed the first filtration through the arc filter frame 1001 can flow downward and enter the filter plate frame 1501. At this time, as the transmission component drives the filter plate frame 1501 to move up and down, the filtration speed can be accelerated while further increasing the contact area between the solution and the filter plate frame 1501, further improving the filtration uniformity, until the solution that has completed the second filtration is discharged through the drainage frame 19.

[0049] In the embodiment of the present invention, specifically, the plurality of arc-shaped transmission blocks 1701 are respectively adapted to the plurality of transmission frames 16;

[0050] In the embodiment of the present invention, specifically, a drive motor 18 is fixedly installed inside the filter body 1, and a transmission belt 1801 is rotatably connected between the output end of the drive motor 18 and the drive shaft 17. The drive motor 18 is an existing device used for driving operation. As the drive motor 18 runs, the drive shaft 17 can be driven to run through the cooperation of the transmission belt 1801.

[0051] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0052] The above is a detailed description of an embodiment of the present invention, but the content is only a preferred embodiment of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the present invention.

Claims

1. A stem cell filtration separator with high uniformity, comprising a filter body (1), characterized in that: A mixing and separation box (5) is installed inside the filter body (1), a liquid inlet hopper (4) is fixed on the top surface of the filter body (1), a liquid inlet pipe (401) is connected between the liquid inlet hopper (4) and the mixing and separation box (5) for conveying the solution, and the mixing and separation box (5) is used to mix the solution and decompose the stem cells; A filter cartridge (9) is fixedly installed inside the filter body (1), and the installation position of the filter cartridge (9) is located below the mixing and separation box (5). A transmission disc frame (10) is rotatably installed inside the filter cartridge (9), and an arc-shaped filter frame (1001) is fixedly installed inside the transmission disc frame (10), and the arc-shaped filter frame (1001) is used to perform a first filtering operation on the solution; The bottom of the mixing and separation box (5) is connected to a drain pipe (7), the end of which extends to the arc-shaped filter frame (1001) and is used to transport the solution to the inside of the running arc-shaped filter frame (1001); An assembly frame (15) is installed below the filter cartridge (9), and a filter plate frame (1501) is inserted into the assembly frame (15). The filter plate frame (1501) is used to perform a second filtering treatment on the solution. A transmission component is provided at the bottom of the assembly frame (15) for driving the assembly frame (15) and the filter plate frame (1501) to reciprocate at the bottom of the filter cartridge (9) to achieve filtering treatment on the solution. A drainage frame (19) is provided below the filter cartridge (9). The drainage frame (19) is fixed to the inner bottom of the filter body (1). The top surface of the drainage frame (19) is provided as an inclined surface for conveying the stem cell solution.

2. The stem cell filtration separator with high uniformity according to claim 1, characterized in that: A cleaning component is provided inside the arc-shaped filter frame (1001) for cleaning impurities remaining inside the arc-shaped filter frame (1001), the cleaning component comprising an arc-shaped liquid pushing plate (13), a collecting plate frame (12) is fixedly installed inside the filter cartridge (9), the arc-shaped liquid pushing plate (13) is fixedly installed at the end of the collecting plate frame (12), a rectangular groove is provided at one end of the collecting plate frame (12) close to the arc-shaped liquid pushing plate (13), and a filter frame (1201) is fixedly installed at the bottom of the collecting plate frame (12).

3. The stem cell filtration separator with high uniformity according to claim 2, characterized in that: The end of the arc-shaped liquid pushing plate (13) extends into the interior of the arc-shaped filter frame (1001), and the arc-shaped liquid pushing plate (13) and the inner wall of the arc-shaped filter frame (1001) are in contact with each other.

4. The stem cell filtration separator with high uniformity according to claim 2, characterized in that: A negative pressure device (14) is fixedly installed inside the filter cartridge (9), and a conveying pipe is connected between the negative pressure device (14) and the inside of the collecting plate frame (12) for extracting the collected impurities.

5. The stem cell filtration separator with high uniformity according to claim 1, characterized in that: A sealing door (2) is rotatably connected to the side of the filter body (1), a control panel (101) is fixedly mounted on the outside of the filter body (1), and a liquid outlet (3) is provided on the side of the filter body (1), and the liquid outlet (3) is kept flush with the end of the liquid drain frame (19).

6. The stem cell filtration separator with high uniformity according to claim 1, characterized in that: A stirring shaft (6) is rotatably mounted inside the mixing and separation box (5), a plurality of stirring blades (601) are fixedly mounted outside the mixing and separation box (5), a servo motor (8) is fixedly mounted outside the mixing and separation box (5), a transmission chain (801) is connected between the output end of the servo motor (8) and the end of the stirring shaft (6), and an electric butterfly valve (701) is mounted inside the discharge pipe (7).

7. The stem cell filtration separator with high uniformity according to claim 1, characterized in that: A driving assembly is provided on the outer side of the filter cartridge (9) for driving the transmission disc frame (10) to rotate inside the filter cartridge (9); a fixing frame (901) is fixedly sleeved on the outer side of the filter cartridge (9); the fixing frame (901) is fixedly installed inside the filter body (1); the driving assembly comprises a driving gear (1101); a driving motor (11) is fixedly installed on the outer side of the filter cartridge (9); the driving gear (1101) is fixedly sleeved on the output end of the driving motor (11); a driving gear ring (1002) is fixedly installed on the top surface of the transmission disc frame (10); the driving gear (1101) and the driving gear ring (1002) are meshed with each other.

8. The stem cell filtration separator with high uniformity according to claim 1, characterized in that: The transmission assembly comprises a transmission frame (16), wherein there are two transmission frames (16) and both are fixed at the bottom of the assembly frame (15); one end of the assembly frame (15) away from the transmission frame (16) is rotatably connected to the inner wall of the filter body (1); a transmission shaft (17) is rotatably mounted inside the filter body (1); a plurality of arc-shaped transmission blocks (1701) are fixedly sleeved on the outer side of the transmission shaft (17); the arc-shaped transmission blocks (1701) are respectively located inside corresponding transmission frames (16) and are used to lift up the transmission frames (16).

9. The stem cell filtration separator with high uniformity according to claim 8, characterized in that: The plurality of arc-shaped transmission blocks (1701) are respectively adapted to fit with the plurality of transmission frames (16).

10. The stem cell filtration separator with high uniformity according to claim 8, characterized in that: A second driving motor (18) is fixedly installed inside the filter body (1), and a transmission belt (1801) is rotatably connected between the output end of the second driving motor (18) and the transmission shaft (17).

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