Compound microbial agent for liquid dung treatment as well as preparation method and application of compound microbial agent

Abstract

The invention belongs to the technical field of composite microbial agents, and particularly relates to a composite microbial agent for liquid dung treatment and a preparation method and application thereof. The compound microbial agent is a mixed microbial agent of streptomyces isothiocyanate (HBU11), aspergillus niger (Aspergillus niger) HBUN19, bacillus subtilis (HBU29) and Hydrogenobacterium bissanensis HBU34, and the compound microbial agent is a mixed microbial agent of bacillus subtilis (HBU19), bacillus subtilis (HBU29), bacillus subtilis (HBU34), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29), bacillus subtilis (HBU29) and bacillus subtilis (HBU34). The compound microbial agent is efficient, non-toxic and biodegradable, and can effectively degrade organic matters in the liquid dung and remarkably reduce the content of pollutants such as ammonia nitrogen and total phosphorus in the liquid dung through compound use of various efficient strains, so that harmless treatment of the liquid dung is realized.

Description

Technical Field

[0001] The invention belongs to the technical field of composite microbial agents, and specifically relates to a composite microbial agent for manure water treatment, and a preparation method and application thereof. Background Art

[0002] With the development of animal husbandry, a large amount of waste manure and water has been generated. At present, the treatment of livestock and poultry manure and sewage in large-scale farms in my country is basically a model of direct utilization after solid-liquid separation. After collecting fresh manure and sewage from dairy farms, the breeding company uniformly transports them to the sewage collection tank. After solid-liquid separation in the sewage collection tank, the cow manure and sewage are discharged into the storage tank. When it reaches a certain amount, it is directly discharged into the crop planting area for irrigation. This method of direct utilization after solid-liquid separation is easy to pollute the surrounding environment, and the cow manure and sewage obtained under this mode is basically untreated raw liquid, and its manure liquid is easy to contain pathogens and parasite eggs. These wastes contain a large amount of organic matter, nitrogen, phosphorus and other nutrients. If they are discharged directly without treatment, they will not only cause environmental pollution, but also affect the aquatic ecosystem. Therefore, how to effectively treat manure and sewage is an important topic in the current field of environmental protection.

[0003] Existing manure treatment technologies usually use physical, chemical, and biological methods. Among them, biological treatment has been widely used due to its low cost, good treatment effect, and environmental protection. However, the bacterial agents currently on the market often have problems such as poor adaptability and low treatment efficiency. Therefore, it is urgent to develop a new and efficient bacterial agent for efficient treatment of manure. Summary of the invention

[0004] The object of the present invention is to provide a novel composite microbial agent for manure water treatment, and a preparation method and application thereof. The composite microbial agent is efficient, non-toxic and biodegradable. Through the combined use of multiple high-efficiency strains, it can effectively degrade organic matter in manure water and significantly reduce the content of pollutants such as ammonia nitrogen and total phosphorus therein, thereby achieving harmless treatment of manure water.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] The invention provides a composite microbial agent for sewage treatment. The composite microbial agent is a mixed agent of Streptomyces althioticus HBU11, Aspergillus niger HBUN19, Bacillus subtilis HBU29 and Hydrogenophaga bisanensis HBU34.

[0007] Further, the Streptomyces isothioic acid HBU11 was deposited in the General Microbiological Center (CGMCC) of the China Microbiological Culture Collection Administration on October 28, 2024, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No: 32381.

[0008] The Aspergillus niger HBUN19 was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration (CGMCC) on October 28, 2024, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No: 41558.

[0009] The Bacillus subtilis HBU29 was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration (CGMCC) on October 28, 2024, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No: 32382.

[0010] The Hydrogenophaga bisanensis HBU34 was deposited in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Administration on October 28, 2024, at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number: CGMCC No: 32384.

[0011] Furthermore, in the above-mentioned composite microbial agent for sewage treatment, the ratio of the spore count of Streptomyces isothiocyanate HBU11 spores, the spore count of Aspergillus niger HBUN19 spores, the effective viable bacteria count of Bacillus subtilis HBU29 fermentation broth and the effective viable bacteria count of Hydrogenophaga bisanensis HBU34 fermentation broth is 1:1:1:1, and the effective viable bacteria count of each species is ≥1×108CFU / mL.

[0012] The preparation method of the composite microbial agent for sewage treatment comprises the following steps:

[0013] (1) taking frozen Bacillus subtilis HBU29 strain and Hydrogenophaga bisanensis HBU34 strain and inoculating them into sterilized LB liquid medium respectively, and culturing them under shaking conditions at 37° C. for 24 hours to obtain seed bacterial liquid; taking the seed bacterial liquid at a volume ratio of 1% and inoculating it into sterilized and cooled LB medium, and fermenting and culturing it in a shaking incubator for 48 hours to obtain fermentation liquids of Bacillus subtilis HBU29 and Hydrogenophaga bisanensis HBU34 respectively;

[0014] (2) taking frozen and stored Streptomyces disulfide HBU11 spores and Aspergillus niger HBUN19 spores and inoculating them into sterilized Gao's No. 1 liquid medium and PDA liquid medium respectively, and culturing them under shaking conditions at 30° C. for 12 hours to obtain seed culture liquid; applying the seed culture liquid of Streptomyces disulfide HBU11 on Gao's No. 1 solid plate or eggplant bottle, and applying the seed culture liquid of Aspergillus niger HBUN19 on PDA solid plate or eggplant bottle, culturing at 28-30° C. for 5-6 days, and then washing and collecting the spores of Streptomyces disulfide HBU11 and Aspergillus niger HBUN19;

[0015] (3) By microscopic counting, the fermentation broths of Bacillus subtilis HBU29 and Hydrogenophaga bisanensis HBU34, spores of Streptomyces isothiocyanate HBU11 and spores of Aspergillus niger HBUN19 were combined in a ratio of 1:1:1:1 of effective viable bacteria or spore counts to obtain a composite microbial agent for sewage treatment.

[0016] The present invention also provides application of the composite microbial agent for manure water treatment in the degradation treatment of manure water.

[0017] Furthermore, during the degradation treatment of manure water, activated carbon is first added into the manure water at an amount of 10 g / L, and then 0.5% to 1.25% by volume of the composite microbial agent for manure water treatment according to claim 1 is added, and continuous aeration treatment is carried out for 8 to 10 days under pH 7 to 9.

[0018] Preferably, the best degradation effect of manure water is achieved when 10 g / L of activated carbon and 1.25% of the composite microbial agent of the present invention are added and aeration treatment is carried out continuously for 10 days in a slightly alkaline environment.

[0019] Furthermore, the manure water is cow dung water.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The invention obtains a composite microbial agent prepared by Streptomyces althioticus HBU11, Aspergillus niger HBUN19, Bacillus subtilis HBU29 and Hydrogenophaga bisanensis HBU34 by screening fermented bedding mixed with 30% sawdust, 60% lees, 10% wheat straw and cow feces. The composite microbial agent has low production cost, strong bacterial colony survival ability, easy cultivation, fast reproduction, strong adaptability to the environment and natural evolution and other characteristics.

[0022] The composite microorganisms of the present invention can be applied to a variety of wastewater environments and have a wide range of applications. The composite microbial agent of the present invention is used to degrade manure water, and the removal effects of BOD and COD in high-concentration manure water can reach 90.89% and 91.64% respectively within 8 to 10 days. The treated manure water fully meets the national comprehensive sewage discharge standards, which is of great significance for reducing the processing load of the back-end process and realizing the harmless treatment of manure water. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is the flow chart of cow dung water treatment.

[0024] Figure 2 The effect of activated carbon dosage on the adsorption of feces and water.

[0025] Figure 3 This is the effect of the percentage of composite microbial agents on the adsorption effect of cow dung wastewater.

[0026] Figure 4 The effect of different pH values ​​on the adsorption of cow dung and dung water.

[0027] Figure 5 The effects of different aeration treatments on manure treatment, where CK represents the original state, Figure A represents closed treatment, Figure B represents intermittent aeration, and Figure C represents continuous aeration. DETAILED DESCRIPTION

[0028] The present invention will be further described below in conjunction with embodiments, but the present invention is not limited thereto.

[0029] Example 1

[0030] Screening of compound microbial agents

[0031] 1. Enrichment, separation and purification of functional bacteria

[0032] The unsterilized mixture (30% sawdust, 60% distiller's grains and 10% wheat straw) and cow manure were mixed evenly and added to a large test tube with a diameter of 30.36 mm and a length of 200 mm, and then placed in a 37°C incubator for fermentation for 30 days. Water was added every 7 days to keep the moisture content at 50%. The fermented mixture was used as the test material for strain screening to screen strains that efficiently remove ammonia and hydrogen sulfide and efficiently degrade lignin and cellulose.

[0033] Take 10 g of the fermented litter and add it to 90 mL of sterile water, shake it for 30 min, let it stand for 30 s, take 10 mL of the supernatant and add it to 100 mL of liquid NH3 selective medium (5 g of glucose,

[0034] (NH4)2SO4 2.0g, NaCl 2g, FeSO4·7H2O 0.4g, MgSO4·7H2O 0.5g, K2HPO4

[0035] 1.0g), H2S selective medium (KH2PO4 1.2g, K2HPO4 1.2g, NH4Cl 0.4g, MgCl2·6H2O0.2g, FeCl3 0.01g, Na2S2O3·5H2O 8.0g), sodium carboxymethyl cellulose medium (MgSO4 0.5g, (NH4)2SO42g, K2HPO4 1g, NaCl 0.5g, sodium carboxymethyl cellulose 5g) and alkaline lignin medium (MgSO4 0.5g, (NH4)2SO4 2g, K2HPO4 1g, NaCl 0.5g, alkaline lignin 5g), 37°C, 180rpm culture for 7 days, continuous enrichment three times. The enriched bacterial solution was appropriately diluted, spread on the corresponding solid selection plate, and cultured at 37°C. Pick a single colony with good growth for streak purification.

[0036] 2. Initial screening of functional strains

[0037] 200 pure cultures were isolated and purified from four selective culture plates. These strains were initially screened to obtain 49 strains, including 24 bacteria, 15 actinomycetes and 10 fungi.

[0038] 3. Functional strain rescreening and species identification

[0039] All 49 strains obtained in the initial screening were further screened, and after the determination of ammonia, hydrogen sulfide, cellulose and lignin degradation rates, 10 strains with good ammonia and hydrogen sulfide degradation effects and 10 strains with good cellulose and lignin degradation effects were obtained; the 20 strains obtained in the re-screening were used for fecal water treatment tests, and 4 strains with better effects were selected from different microbial groups for species identification and subsequent bacterial agent composite tests.

[0040] The 16SrDNA bacterial species identification and ITS fungal species identification methods were used to identify the four strains as Streptomyces althioticus HBU11, Aspergillus niger HBUN19, Bacillus subtilis HBU29 and Hydrogenophaga bisanensis HBU34. The composite microbial agent of the present invention is prepared from these four strains.

[0041] Among them, the Streptomyces isothioic acid HBU11 was deposited in the General Microbiological Center (CGMCC) of the China Microbiological Culture Collection Administration on October 28, 2024, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No: 32381.

[0042] The Aspergillus niger HBUN19 was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration (CGMCC) on October 28, 2024, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No: 41558.

[0043] The Bacillus subtilis HBU29 was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration (CGMCC) on October 28, 2024, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No: 32382.

[0044] The Hydrogenophaga bisanensis HBU34 was deposited in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Administration on October 28, 2024, at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number: CGMCC No: 32384.

[0045] Example 2

[0046] The preparation of the composite microbial agent of the present invention comprises the following steps:

[0047] (1) Take the frozen strains HBU29 and HBU34, inoculate them into sterilized LB liquid culture medium respectively, and shake culture them at 37°C and 180 rpm for 24 hours to obtain seed bacterial liquid. Take the seed bacterial liquid at a volume ratio of 1% and inoculate it into the sterilized and cooled LB medium, and ferment and culture it in a shaker for 48 hours at a temperature of 37°C and a speed of 180 rpm to obtain the fermentation liquid of the two strains;

[0048] (2) Take the frozen HBU11 strain spores and inoculate them into the sterilized Gao's No. 1 liquid culture medium, and culture them at 30°C and 180 rpm for 12 hours to obtain the seed culture liquid. Take an appropriate amount of the culture liquid and spread it on Gao's No. 1 solid plate or eggplant bottle, culture it at 30°C for 5-6 days, and then wash and collect the spores;

[0049] (3) Take the frozen HBUN19 strain spores and inoculate them into the sterilized PDA liquid culture medium. After shaking and culturing at 30°C and 180 rpm for 12 hours, obtain the seed bacterial solution. Take an appropriate amount of the bacterial solution and spread it on the PDA solid plate or eggplant bottle. After culturing at 28°C for 5-6 days, wash and collect the spores;

[0050] (4) Through microscopic counting, the liquid composite bacterial agent was obtained by combining the bacterial count or spore count in a ratio of 1:1:1:1 and stored at 4°C for future use. There was no antagonism between the strains, and the effective viable bacterial count of each strain was determined to be greater than 1×10 8 CFU / mL.

[0051] Example 3

[0052] The composite microbial agent for treating manure water of the present invention is used in the degradation treatment of manure water.

[0053] 1. Original sample analysis

[0054] The experimental raw materials of this embodiment come from a certain animal husbandry company, and the original samples are collected from the feces storage tank of the breeding farm. The quality analysis results of the original sample liquid feces are shown in Table 1.

[0055] Table 1 Liquid feces and sewage quality analysis

[0056] Main indicators Value (mg / L) COD 6740.42 BOD 4128.36

[0057] 2. Process

[0058] According to the actual situation, the large particles of impurities in the original cow dung water are first removed. Since there are still many small and medium-sized unknown particles attached to the surface of the dung water, a certain amount of activated carbon is first added for adsorption treatment. After the treatment, the composite microbial agent prepared in Example 2 is added to the cow dung water sample for further degradation treatment. The specific cow dung water treatment process is as follows: Figure 1 shown.

[0059] 2.1 Activated carbon adsorption treatment

[0060] Take 7 portions of 1L of manure water from the original sample of cow dung water from which sludge and large particles have been removed as treated samples, and mark the 7 samples as A, B, C, D, E, F, and G. Different amounts of activated carbon were added, and the amounts of AG were 2g / L, 4g / L, 6g / L, 8g / L, 10g / L, 12g / L, and 14g / L, respectively. Seal the conical flask containing the sample solution with a plastic film, place it on a shaker and shake it for 8 hours, let it stand for 30 minutes after the shaking ends, take the supernatant of the sample solution in each conical flask for the determination of COD and BOD content, and determine the optimal amount of activated carbon based on the determination results.

[0061] Table 2 Effect of activated carbon dosage on adsorption effect

[0062]

[0063] From Table 2 and Figure 2As shown in the figure, with the addition of activated carbon, the COD and BOD values ​​in the manure water are constantly decreasing. When the amount of activated carbon added is 10g / L, it tends to a stable state. After the amount added is greater than 10g / L, the COD and BOD values ​​no longer increase, indicating that the optimal amount of activated carbon for the treatment of manure water is 10g / L.

[0064] 2.2 Effect of bacterial liquid volume percentage on cow dung wastewater

[0065] According to the method in 2.1, take cow dung sewage into conical bottles, mark them with numbers, add different amounts of homemade composite microbial agents to AG, and add the corresponding volume percentages of 0.5%, 0.75%, 1%, 1.25%, 1.5%, 1.75%, and 2% of the agents. Treat for 7 days, and shake them manually during the treatment process to allow the bacterial solution to fully react with the dung water. As the degradation treatment proceeds, measure and record the COD and BOD contents in each treatment to obtain the optimal amount of microbial agents for cow dung water treatment.

[0066] Table 3 Effect of percentage of composite microbial agent on adsorption of cow dung sewage

[0067]

[0068] From Table 3 and Figure 3 It can be seen that with the increase of the input of microbial agents, the values ​​of COD and BOD in the manure water are gradually decreasing. When the input of microbial agents is 1.25%, a critical point appears. When the input is greater than 1.25%, the content of COD and BOD in the manure water also increases with the increase of the input of agents. When the volume ratio of the composite microbial agent is 1.25%, the treatment of manure water reaches the best state; the input amount greater than 1.25% will cause the content of microbial agents in manure water to exceed the normal capacity, and the balance between the microbial community and the water environment will be broken, thereby affecting the decomposition effect. It can be concluded from the test results that the optimal volume percentage of composite microbial agents that can be used for manure water treatment is 1.25%.

[0069] 2.3 Effect of pH value on treatment of cow dung wastewater

[0070] The cow dung and manure water sample liquid treated with the optimal amount of composite microbial agent was selected, and the same type of sampling was numbered based on the sample liquid. The pH value of the sample liquid was adjusted within the range of 4-10, and the COD and BOD contents in the sample liquid were measured under different pH value ranges, and finally the pH value with the best manure water treatment effect was determined.

[0071] Table 4 Treatment results of cow dung water at different pH values

[0072]

[0073] From Table 4 and Figure 4 It can be seen that with the change of pH, the COD and BOD values ​​in the manure water are also changing. As the pH gradually increases, the adsorption and degradation of the manure water also gradually increases. When the pH is 8, a different trend of change appears; therefore, it can be concluded that the optimal pH for treating cow dung water in this experiment is neutral to slightly alkaline.

[0074] 2.4 Effect of time on treatment of cow dung wastewater

[0075] The optimal amount of compound microbial agent and the optimal pH value for treating cow dung and manure water have been determined before. The optimal treatment time for manure water treatment is further determined by selecting manure water samples that have been determined to be optimal. The treated manure water samples are renumbered and marked, and the COD and BOD values ​​in the manure water are measured in the morning, noon and evening every day until the optimal treatment time is determined.

[0076] Table 5 Treatment results of cow dung water at different times

[0077] Serial number Time (d) COD COD removal rate (%) BOD BOD removal rate (%) 1 1 4851.08 28.03 3627.59 12.13 2 2 3560.29 47.18 3478.56 15.74 3 3 2746.72 59.25 3288.65 20.34 4 4 1739.70 74.19 3007.92 27.14 5 5 1263.83 81.25 2516.24 39.05 6 6 990.84 85.30 2287.52 44.59 7 7 777.17 88.47 1585.70 61.59 8 8 508.23 92.46 1401.58 66.05 9 9 515.64 92.35 844.25 79.55 10 10 507.55 92.47 441.73 89.30 11 11 505.53 92.50 394.26 90.45 12 12 508.90 92.45 409.95 90.07 13 13 505.53 92.50 397.97 90.36

[0078] It can be concluded from Table 5 that the degradation effect of cow dung water increases with time, and correspondingly, the COD and BOD values ​​in the water decrease with time. Before the 10th day of treatment, the treatment effect was obvious, with the COD value decreasing from 6740.42 to 507.55 and the BOD value decreasing from 4128.36 to 441.73, and the removal rates of the two reaching 92.47% and 89.30%; the treatment effect was the best on the 10th day, and after the 10th day, the treatment effect did not change significantly with the increase of time, and was basically in a flat state.

[0079] 2.5 Effect of aeration on the treatment of cow dung wastewater

[0080] On the basis of the previously determined indicators, the cow dung and manure water samples were treated with aeration and non-aeration to explore the changes in the manure water under these two treatment methods. The degradation effects of continuous aeration and intermittent aeration were compared on the basis of aeration treatment. Finally, the best treatment method was determined through the COD and BOD values.

[0081] Table 6 Effect of aeration on cow dung and manure water treatment under optimal conditions

[0082]

[0083] From Table 6 and Figure 5It can be concluded that the treatment effect of manure water under aeration conditions is obvious. Compared with closed treatment, aeration treatment has more obvious advantages, and the COD and BOD contents in manure water have decreased significantly. Under aeration treatment, continuous aeration is better than intermittent aeration treatment, which can reduce the COD and BOD values ​​in manure water to 536.8 and 376.3 mg / L. The reason may be that aerobic microorganisms play a dominant role in decomposing insoluble organic matter in manure water.

[0084] In summary, after a series of process flows such as adding activated carbon - determining the optimal volume percentage of composite microbial agents - determining the optimal treatment environment (pH) - determining the optimal treatment time - continuous aeration treatment, the BOD and COD values ​​of the original dung water were reduced to 376.3 and 563.8 mg / L. Compared with my country's comprehensive sewage discharge standard GB8978-1996, cow dung water belongs to its second-class sewage discharge. The state stipulates that the COD and BOD values ​​of urban tertiary sewage discharge cannot exceed 600 mg / L. The degradation treatment of cow dung water in this experiment meets the national emission requirements and has met the standards.

[0085] From the results of the above-mentioned process treatments, it can be concluded that the treatment steps of each scheme have a significant effect on the degradation and purification of cow dung and manure. For the large-scale farm manure treatment, the manure degradation scheme based on the high-efficiency composite microbial agent of the present invention is: adding 10g / L of activated carbon, 1.25% of the composite microbial agent of the present invention, and continuously aerating for 10 days in a slightly alkaline environment to achieve the best degradation effect of manure.

[0086] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composite microbial agent for sewage treatment, characterized in that: The composite microbial agent is Streptomyces isothiurans ( Streptomyces althioticus )HBU11, Aspergillus niger ( Aspergillus niger ) HBUN19, Bacillus subtilis ( Bacillus subtilis )HBU29 and Hydrogenophaga bisanensis Mixed inoculant of HBU34.

2. The composite microbial agent for sewage treatment according to claim 1, characterized in that: The streptomyces isothioic acid HBU11 was deposited in the General Microbiology Center (CGMCC) of the China National Microbiological Culture Collection Administration on October 28, 2024, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No. 32381; the Aspergillus niger HBUN19 was deposited in the General Microbiology Center (CGMCC) of the China National Microbiological Culture Collection Administration on October 28, 2024, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No. 41558; the Bacillus subtilis HBU29 was deposited in the General Microbiology Center (CGMCC) of the China National Microbiological Culture Collection Administration on October 28, 2024, at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No. 32382; Hydrogenophaga bisanensis HBU34 was deposited on October 28, 2024 at the General Microbiology Center of China Culture Collection Administration (CGMCC), address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number: CGMCC No: 32384.

3. The composite microbial agent for sewage treatment according to claim 1, characterized in that: In the composite microbial agent, the number of spores of Streptomyces isothioic acid HBU11, the number of spores of Aspergillus niger HBUN19, the number of effective live bacteria in the fermentation liquid of Bacillus subtilis HBU29 and Hydrogenophaga bisanensis The ratio of effective viable bacteria count in HBU34 fermentation broth was 1:1:1:1, and the effective viable bacteria count of each strain was ≥1×10 8 CFU / mL.

4. The method for preparing the composite microbial agent for sewage treatment according to claim 1, characterized in that: The following steps are involved: (1) Take the frozen Bacillus subtilis HBU29 strain and Hydrogenophaga bisanensis The HBU34 strain was inoculated into the sterilized LB liquid medium and cultured at 37°C for 24 hours to obtain the seed bacterial solution; the seed bacterial solution was inoculated into the sterilized and cooled LB medium at a volume ratio of 1%, and fermented and cultured in a shaker for 48 hours to obtain Bacillus subtilis HBU29 and Hydrogenophaga bisanensis Fermentation broth of HBU34; (2) Take frozen and stored Streptomyces disulfide HBU11 spores and Aspergillus niger HBUN19 spores and inoculate them into sterilized Gao's No. 1 liquid medium and PDA liquid medium respectively, and culture them at 30°C under shaking conditions for 12 hours to obtain seed culture liquid; spread the seed culture liquid of Streptomyces disulfide HBU11 on Gao's No. 1 solid plate or eggplant bottle, and spread the seed culture liquid of Aspergillus niger HBUN19 on PDA solid plate or eggplant bottle, culture them at 28~30°C for 5-6 days, and then wash and collect the spores of Streptomyces disulfide HBU11 and Aspergillus niger HBUN19; (3) By microscopic counting, the number of effective viable bacteria or spores was 1:1:1:1, and the number of Bacillus subtilis HBU29 and Hydrogenophaga bisanensis The fermentation broth of HBU34, spores of Streptomyces isothiocyanate HBU11 and spores of Aspergillus niger HBUN19 were used to obtain a composite microbial agent for sewage treatment.

5. Use of the composite microbial agent for manure water treatment according to claim 1 in the degradation treatment of manure water.

6. The use according to claim 5, characterized in that: During the degradation treatment of manure water, activated carbon is first added into the manure water at an amount of 10 g / L, and then 0.5% to 1.25% by volume of the composite microbial agent for manure water treatment according to claim 1 is added, and continuous aeration treatment is performed for 8 to 10 days under pH 7 to 9.

7. The use according to claim 5, characterized in that: The dung water is cow dung dung water.

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