Algal oil protein composition for reducing fishy smell and increasing freshness of largemouth bass meat as well as preparation method and application of algal oil protein composition

Abstract

The invention belongs to the technical field of aquaculture, and discloses an algal oil protein composition for reducing fishy smell and increasing freshness of largemouth bass meat as well as a preparation method and application of the algal oil protein composition. The algal oil protein composition comprises 2-10 parts of algal oil powder, 15-25 parts of porcine spray-dried animal blood cells and 18-32 parts of dephenolized cottonseed protein. The algal oil powder is prepared by mixing cassava porous starch, sucrose fatty ester and DHA algal oil, dissolving the mixture in distilled water at 50-90 DEG C, stirring, homogenizing under the pressure of 60-100 MPa to obtain a stable emulsion, and performing spray drying. According to the invention, two animal and plant protein sources, namely porcine spray-dried blood cells and degossypolized cottonseed protein, which are relatively low in price and complementary in amino acid component are selected, and the three components are scientifically matched to play a synergistic effect by virtue of a microcapsule method. The composition can promote growth of micropterus salmoides, increase the content of inosinic acid in fish meat, achieve the effects of reducing cost, increasing efficiency and improving the delicate flavor of the fish meat, and can be applied to the field of micropterus salmoides culture.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to an algae oil protein composition for reducing the fishy smell and increasing the freshness of largemouth bass meat, and a preparation method and application thereof. Background Art

[0002] With the rapid development of intensive farming, the demand for fish meal in aquaculture has increased dramatically. However, due to the impact of overfishing and the El Niño phenomenon, the total output of fish meal has decreased year by year, while the price has continued to rise. These factors have seriously restricted the sustainable development of aquaculture. At present, animal and plant protein sources are considered to be the most effective fish meal substitutes, which can significantly reduce the amount of fish meal in feed and improve the economic benefits of farming. However, after replacing fish meal, these raw materials will have a serious negative impact on the quality of fish meat, especially the taste and flavor, such as the increase of fish smell, which in turn affects people's desire to buy the aquatic product. The taste of fish meat mainly depends on amino acids and nucleotides, among which inosinic acid (IMP) has the strongest umami effect, and the higher the inosinic acid content in muscle, the lower the fish smell of meat and the more delicious the taste. In view of this, the development of fish meal substitute products that can increase the IMP content of fish meat will help improve the quality of aquatic products and increase the economic benefits of farmers. However, so far, there has been no application report of related products.

[0003] Porcine hemoglobin powder is made by separating fresh pig blood from plasma, hydrolyzing it with enzymes, and then drying it. Its protein content is greater than 80%; dephenolized cottonseed protein is a plant high-protein product made by depilating and shelling cottonseeds and then removing the cottonphenol. Its protein content is greater than 50%. Both are often used as alternative sources of fish meal in feed. However, when porcine hemoglobin powder or dephenolized cottonseed protein is used alone to replace fish meal in feed, the fish's feed utilization rate will be significantly reduced due to its own amino acid imbalance, such as low content of arginine, lysine and methionine. In addition, compared with fish meal, both lack n-3 polyunsaturated fatty acids (DHA) in their nutritional components, which further restricts their use in fish feed.

[0004] DHA is extremely unstable in structure and easily oxidized and deteriorated when heated, which endangers the health of living organisms. In addition, DHA oil is not easy to mix with other feed ingredients. Therefore, the application of directly adding exogenous DHA oil to feed is greatly limited. Microencapsulation technology is a technology that uses special materials as wall materials to form a semi-enclosed or enclosed space to bury solid, liquid and other substances. It can effectively retain the original nutrients and biological activity of the core material and improve the stability of the core material. However, there have been no reports on the preparation of DHA oil powder with the help of microencapsulation technology and its addition to aquatic feed. Summary of the invention

[0005] In order to solve the deficiencies and shortcomings of the above-mentioned prior art, the primary purpose of the present invention is to provide an algae oil protein composition for reducing the fishy smell and increasing the freshness of largemouth black bass meat. The composition significantly increases the inosinic acid content in fish meat by replacing a high proportion of fish meal in sea bass feed, thereby achieving the effect of reducing costs, increasing efficiency and improving the quality of fish meat.

[0006] Another object of the present invention is to provide a method for preparing the algae oil protein composition. The method uses microencapsulation technology to prepare algae oil powder, and fully mixes it with porcine hemoglobin powder and dephenolized cottonseed protein to obtain the algae oil protein composition.

[0007] Another object of the present invention is to provide applications of the algae oil protein composition.

[0008] The purpose of the present invention is achieved through the following technical solutions:

[0009] A algae oil protein composition for reducing the fishy smell and increasing the freshness of largemouth bass meat comprises 2-10 parts of algae oil powder, 15-25 parts of porcine hemoglobin powder and 18-32 parts of dephenolized cottonseed protein; the algae oil powder is prepared by mixing cassava porous starch, sucrose fatty ester and DHA algae oil, dissolving in 50-90°C distilled water with stirring, homogenizing under a pressure of 60-100MPa to obtain a stable emulsion, and spray drying.

[0010] Preferably, the algae oil protein composition comprises 5 to 9 parts of algae oil powder, 16 to 22 parts of porcine hemoglobin powder and 20 to 29 parts of dephenolized cottonseed protein.

[0011] More preferably, the algae oil protein composition comprises 7-8 parts of algae oil powder, 17-20 parts of porcine hemoglobin powder and 25-28 parts of dephenolized cottonseed protein.

[0012] Preferably, the concentration of cassava porous starch in the stable emulsion is 5-25wt%, the concentration of sucrose fatty ester in the stable emulsion is 10-35wt%, and the concentration of DHA algae oil in the stable emulsion is 5-22wt%.

[0013] Preferably, the volume ratio of the total mass of the cassava porous starch, sucrose fatty ester and DHA algae oil to distilled water is (1-41) g: (50-500) mL.

[0014] The preparation method of the algae oil protein composition for reducing the fishy smell and increasing the freshness of largemouth bass meat comprises the following steps:

[0015] S1. Mix cassava porous starch, sucrose fatty ester and DHA algae oil, dissolve in 50-90°C distilled water and stir, homogenize at 60-100MPa to obtain a stable emulsion, spray dry the emulsion, and the inlet and outlet air temperatures are 120-160°C and 60-80°C, respectively, at a flow rate of 500-800mL / h to obtain algae oil powder;

[0016] S2. The algae oil powder, which was crushed at 35-40° C. and passed through a 80-150 mesh sieve, the porcine hemoglobin powder and the dephenolized cottonseed protein were mixed evenly by a step-by-step mixing method to obtain an algae oil protein composition.

[0017] Preferably, the stirring speed in step S1 is 2000-4500 r / min, and the stirring time is 12-24 h.

[0018] Preferably, the homogenization time in step S1 is 40 to 120 minutes.

[0019] The algae oil protein composition for reducing the fishy smell and increasing the freshness of largemouth bass meat is used in the field of largemouth bass breeding.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The present invention selects low-priced animal and plant protein sources with complementary amino acid components, namely, porcine hemoglobin powder and dephenolized cottonseed protein, and adds algae oil powder, which can provide essential fatty acid nutrition required by fish. The three ingredients are scientifically matched to exert synergistic effects, and an algae oil protein composition for largemouth bass breeding is developed.

[0022] 2. The present invention also applies microcapsule technology to the processing of the composition, which greatly retains the nutritional components and biological activity of the algae oil and improves its bioavailability by aquatic animals.

[0023] 3. The present invention replaces the amount of fish meal in sea bass feed in a high proportion, significantly increases the inosinic acid content in fish meat, achieves the effect of reducing costs, increasing efficiency and improving the quality of fish meat. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is the thermal stability of DHA algae oil and algae oil powder in Application Example 1.

[0025] Figure 2 This is the effect of feed in different treatment groups in Application Example 4 on the meat texture indicators of largemouth bass. DETAILED DESCRIPTION

[0026] The content of the present invention is further described below in conjunction with specific examples, but it should not be construed as limiting the present invention. If not specifically indicated, the technical means used in the examples are conventional means well known to those skilled in the art. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0027] Example 1

[0028] 1. Weigh cassava porous starch, sucrose fatty ester and DHA algae oil and dissolve them in 500 mL of 50° C. distilled water to obtain 5 wt% cassava porous starch, 10 wt% sucrose fatty ester and 5 wt% DHA. Stir at 2000 r / min for 12 h, and then homogenize 4 times (10 min each time) at 60 MPa pressure with the help of a high-pressure homogenizer to obtain a uniform stable emulsion. The emulsion is spray-dried with the inlet and outlet temperatures of 120° C. and 80° C., respectively, at a flow rate of 500 mL / h to obtain algae oil powder.

[0029] 2. Use a step-by-step mixing method to fully and evenly mix 2 parts of algae oil powder that has been crushed at 40°C and passed through an 80-mesh sieve, 24 parts of porcine hemoglobin powder, and 24 parts of dephenolized cottonseed protein. This process must ensure that the mixture is uniform and stable, and obtain 50 parts of algae oil protein compositions.

[0030] Example 2

[0031] 1. Weigh cassava porous starch, sucrose fatty ester and DHA algae oil and dissolve them in 500 mL of 55° C. distilled water to obtain 10 wt% cassava porous starch, 12 wt% sucrose fatty ester and 8 wt% DHA. Stir at 2000 r / min for 15 h, and then homogenize 4 times at 60 MPa pressure with the help of a high-pressure homogenizer, each time for 12 min, to obtain a uniform stable emulsion. The emulsion is spray-dried with the inlet and outlet air temperatures of 120° C. and 60° C., respectively, and the flow rate is 500 mL / h to obtain algae oil powder.

[0032] 2. Use a step-by-step mixing method to fully and evenly mix 2 parts of algae oil powder that was crushed at 40°C and passed through a 100-mesh sieve, 16 parts of porcine hemoglobin powder, and 32 parts of dephenolized cottonseed protein. This process must ensure that the mixture is uniform and stable, and obtain 50 parts of algae oil protein compositions.

[0033] Example 3

[0034] 1. Weigh cassava porous starch, sucrose fatty ester and DHA algae oil and dissolve them in 500 mL of 65° C. distilled water to obtain 15 wt% cassava porous starch, 20 wt% sucrose fatty ester and 10 wt% DHA. Stir at 3000 r / min for 20 h, and then homogenize 4 times at 70 MPa pressure with the help of a high-pressure homogenizer for 15 min each time to obtain a uniform stable emulsion. The emulsion is spray-dried with the inlet and outlet air temperatures of 150° C. and 60° C., respectively, at a flow rate of 600 mL / h to obtain algae oil powder.

[0035] 2. Use a step-by-step mixing method to fully and evenly mix 5 parts of algae oil powder that were crushed at 35°C and passed through a 100-mesh sieve, 16 parts of porcine hemoglobin powder, and 29 parts of dephenolized cottonseed protein. This process must ensure that the mixture is uniform and stable, and obtain 50 parts of algae oil protein compositions.

[0036] Example 4

[0037] 1. Weigh cassava porous starch, sucrose fatty ester and DHA algae oil and dissolve them in 500 mL of 75°C distilled water to obtain 20wt% cassava porous starch, 20wt% sucrose fatty ester and 10wt% DHA, stir at 3500r / min for 20h, and then homogenize 4 times at 70MPa pressure with the help of a high-pressure homogenizer, each time for 20min, to obtain a uniform stable emulsion, and spray dry the emulsion, with the inlet and outlet temperatures of 140°C and 60°C respectively, and the flow rate of 600mL / h to obtain algae oil powder.

[0038] 2. Use a step-by-step mixing method to fully mix 5 parts of algae oil powder that have been crushed at 35°C and passed through a 120-mesh sieve, 20 parts of porcine hemoglobin powder, and 25 parts of dephenolized cottonseed protein. This process must ensure that the mixture is uniform and stable, and obtain 50 parts of algae oil protein compositions.

[0039] Example 5

[0040] 1. Weigh cassava porous starch, sucrose fatty ester and DHA algae oil and dissolve them in 500 mL of 80°C distilled water to obtain 25wt% cassava porous starch, 28wt% sucrose fatty ester and 18wt% DHA, stir at 4000r / min for 12h, and then homogenize 4 times at 75MPa pressure with the help of a high-pressure homogenizer, each time for 20min, to obtain a uniform and stable emulsion, and spray dry the emulsion, with the inlet and outlet air temperatures of 150°C and 70°C respectively, and the flow rate of 650mL / h to obtain algae oil powder.

[0041] 2. Use a step-by-step mixing method to fully mix 7 parts of algae oil powder that were crushed at 35°C and passed through a 120-mesh sieve, 25 parts of porcine hemoglobin powder, and 18 parts of dephenolized cottonseed protein. This process must ensure that the mixture is uniform and stable, and obtain 50 parts of algae oil protein compositions.

[0042] Example 6

[0043] 1. Weigh cassava porous starch, sucrose fatty ester and DHA algae oil and dissolve them in 500 mL of 80°C distilled water to obtain 25wt% cassava porous starch, 30wt% sucrose fatty ester and 20wt% DHA, stir at 4000r / min for 20h, and then homogenize 4 times at 80MPa pressure with the help of a high-pressure homogenizer, each time for 30min, to obtain a uniform stable emulsion, and spray dry the emulsion, with the inlet and outlet temperatures of 150°C and 70°C respectively, and the flow rate of 700mL / h to obtain algae oil powder.

[0044] 2. Use a step-by-step mixing method to fully mix 7 parts of algae oil powder that have been crushed at 35°C and passed through a 150-mesh sieve, 20 parts of porcine hemoglobin powder, and 23 parts of dephenolized cottonseed protein. This process must ensure that the mixture is uniform and stable, and obtain 50 parts of algae oil protein compositions.

[0045] Example 7

[0046] 1. Weigh cassava porous starch, sucrose fatty ester and DHA algae oil and dissolve them in 500 mL of 90° C. distilled water to obtain 25 wt% cassava porous starch, 35 wt% sucrose fatty ester and 20 wt% DHA. Stir at 4000 r / min for 24 h, and then homogenize 4 times at a pressure of 100 MPa with the help of a high-pressure homogenizer for 30 min each time to obtain a uniform stable emulsion. The emulsion is spray-dried with the inlet and outlet air temperatures of 160° C. and 60° C., respectively, at a flow rate of 700 mL / h to obtain algae oil powder.

[0047] 2. Use a step-by-step mixing method to fully mix 10 parts of algae oil powder that have been crushed at 35°C and passed through a 150-mesh sieve, 15 parts of porcine hemoglobin powder, and 25 parts of dephenolized cottonseed protein. This process must ensure that the mixture is uniform and stable, and obtain 50 parts of algae oil protein compositions.

[0048] Example 8

[0049] 1. Weigh cassava porous starch, sucrose fatty ester and DHA algae oil and dissolve them in 500 mL of 90° C. distilled water to obtain 25 wt% cassava porous starch, 35 wt% sucrose fatty ester and 22 wt% DHA. Stir at 4500 r / min for 18 h, and then homogenize 4 times at a pressure of 100 MPa with the help of a high-pressure homogenizer for 30 min each time to obtain a uniform stable emulsion. The emulsion is spray-dried with the inlet and outlet air temperatures of 160° C. and 60° C., respectively, at a flow rate of 800 mL / h to obtain algae oil powder.

[0050] 2. Use a step-by-step mixing method to fully mix 8 parts of algae oil powder that were crushed at 35°C and passed through a 150-mesh sieve, 17 parts of porcine hemoglobin powder, and 25 parts of dephenolized cottonseed protein. This process must ensure that the mixture is uniform and stable, and obtain 50 parts of algae oil protein compositions.

[0051] Application Example 1

[0052] Accelerated oxidation experiment of DHA algae oil: 40g of DHA algae oil and algae oil powder of Example 1 were placed in a 100mL stoppered conical flask and kept at 60°C in dark for 24h. The samples were taken and the peroxide value (POV) was determined according to GB / T5009.37-2003 "Analysis Method of Hygienic Standard for Edible Vegetable Oils"; the thiobarbituric acid reactant was determined by trichloroacetic acid extraction method, and the result was calculated as malondialdehyde (MDA).

[0053] Figure 1 The thermal stability of DHA algae oil and algae oil powder in Application Example 1. Figure 1It can be seen that after heating treatment, the peroxide value and malondialdehyde content of algae oil powder are significantly lower than those of DHA algae oil, indicating that algae oil powder has better thermal stability and can better retain the nutritional components and biological activity of algae oil.

[0054] Application Example 2

[0055] The breeding experiment was conducted in the recirculating aquaculture system of South China Agricultural University, where each aquarium had a water volume of 300L. 480 healthy young fish were randomly selected, with an average weight of 35.69±0.03g, for a 12-week breeding experiment. The experiment was divided into 6 groups, with 4 replicates in each group. That is:

[0056] ①Basic group: fed with feed containing 50wt% fish meal;

[0057] ②Porcine hemoglobin replacement group: fed with feed containing 25wt% fish meal + 25wt% porcine hemoglobin;

[0058] ③Dephenolized cottonseed protein replacement group: fed with a feed containing 25wt% fish meal + 25wt% dephenolized cottonseed protein;

[0059] ④ A group of pig hemoglobin and dephenolized cottonseed protein combination replacements: fed with a feed composed of 25wt% fish meal + 25wt% pig hemoglobin and dephenolized cottonseed protein (the mass ratio of pig hemoglobin to dephenolized cottonseed protein was 1:1);

[0060] ⑤Algae oil protein composition 1 replacement group: fed with feed containing 25wt% fish meal + 25wt% algae oil protein composition 1;

[0061] ⑥Algae oil protein composition 2 replacement group: fed with feed containing 25wt% fish meal + 25wt% algae oil protein composition 2.

[0062] Table 1 Formula of experimental feed

[0063]

[0064] The premix was purchased from Guangzhou Feixi Special Aquatic Products Technology Co., Ltd., which contains minerals (g / kg) and vitamins (IU or mg / kg) per kilogram of premix: CuSO4·5H2O 2.0g; FeSO4·7H2O 25g; ZnSO4·7H2O 22g; MnSO4·4H2O 7g; Na2SeO30.04 g; KI0.026g; CoCl2·6H2O 0.1g; VA 900,000IU; VD 200,000IU; VE 4500mg; VK3220mg; VB1320 mg; VB21090 mg; niacin 2800mg; VB52000mg; VB6500 mg; VB 121.6 mg; VC 5000mg; pantothenic acid 1000mg; folic acid 165mg; choline 60000mg; inositol 15000mg.

[0065] During the breeding experiment, the fish were fed twice a day (8:00 and 17:00), and all the fish were starved for 24 hours after the breeding. Then the number and weight of each group of fish were counted. After that, 6 fish were selected from each tank and quickly anesthetized with 100 mg / L MS-222, and their muscle tissue was taken for analysis of inosine and protein content.

[0066] The method for determining the inosinic acid content is as follows: Take 1.25g of fresh meat sample and put it into a petri dish. Cut it into pieces with scissors and put it into a homogenizing tube. Add 4mL of 5vol% perchloric acid to make a homogenate to extract inosinic acid. Transfer the extract into a 10mL centrifuge tube, wash the homogenizing tube with 1mL of 5% perchloric acid, pour it into a centrifuge tube and heat at 3500r min. -1 Centrifuge for 5 minutes, filter the supernatant, precipitate in a 50 mL beaker, shake with 5% perchloric acid for 5 minutes, centrifuge and filter the supernatant, combine with the previous supernatant, and use 5.0 and 0.5 mol L -1 Adjust the pH to 6.5 with sodium hydroxide, transfer to a 25 mL volumetric flask, dilute to volume with distilled water, shake well, and filter with a 0.5 μm filter membrane to obtain the supernatant for HPLC analysis.

[0067] Table 2 Effects of different treatment groups of feed on growth and physiological and biochemical parameters of largemouth bass

[0068]

[0069]

[0070] Note: The same lowercase letters in the same row indicate no significant difference

[0071] Table 2 shows the effects of feeds of different treatment groups on the growth and physiological and biochemical indicators of largemouth bass in Application Example 2. As shown in Table 2, in the fish meal replacement group (i.e., pig hemoglobin replacement group, dephenolized cottonseed protein replacement group, pig hemoglobin and dephenolized cottonseed protein combination replacement group, algae oil protein composition 1 replacement group, and algae oil protein composition 2 replacement group), the algae oil protein composition 2 replacement group feed significantly increased the weight gain rate, specific growth rate, muscle inosine and protein content of largemouth bass, but the feed coefficient was significantly reduced. This shows that the algae oil protein composition 2 replacement group feed is better in improving fish growth, feed utilization, and muscle inosine and protein content.

[0072] Application Example 3

[0073] The breeding experiment was conducted in the recirculating aquaculture system of South China Agricultural University, where each aquarium had a water volume of 300L. 400 healthy young fish were randomly selected, with an average weight of 40.35±0.06)g, and a 12-week breeding experiment was conducted. The experiment was divided into 5 groups, with 4 replicates in each group. That is:

[0074] ① Algal oil protein composition 2 replacement group: fed with feed containing 25wt% fish meal + 25wt% algal oil protein composition 2;

[0075] ②Algae oil protein composition 3 replacement group: fed with feed containing 25wt% fish meal + 25wt% algae oil protein composition 3;

[0076] ③Algae oil protein composition 4 replacement group: fed with feed containing 25wt% fish meal + 25wt% algae oil protein composition 4;

[0077] ④Algae oil protein composition 5 replacement group: fed with feed containing 25wt% fish meal + 25wt% algae oil protein composition 5;

[0078] ⑤ Algal oil protein composition 6 replacement group: fed with feed containing 25wt% fish meal + 25wt% algal oil protein composition 6.

[0079] The feed formula of the basic group was the same as that of Application Example 2. During the breeding experiment, the fish were fed twice a day (8:00 and 17:00). After the breeding, all the fish were starved for 24 hours, and then the number and weight of fish in each group were counted. After that, 6 fish were selected from each tank and quickly anesthetized with 100 mg / L MS-222, and their muscle tissues were taken for analysis of inosinic acid and protein content.

[0080] Table 3 Effects of different treatment groups of feed on growth and physiological and biochemical parameters of largemouth bass

[0081]

[0082] Note: The same lowercase letters in the same row indicate no significant difference

[0083] Table 3 shows the effects of feeds of different treatment groups on the growth and physiological and biochemical indicators of largemouth bass in Application Example 3. As shown in Table 3, the feed of the algae oil protein composition 6 replacement group significantly increased the weight gain rate, specific growth rate, muscle inosine and protein content of largemouth bass, but the feed conversion ratio was significantly reduced. This shows that the feed of the algae oil protein composition 6 replacement group is better in improving fish growth, feed utilization, and muscle inosine and protein content.

[0084] Application Example 4

[0085] The breeding experiment was conducted in the recirculating aquaculture system of South China Agricultural University, where each aquarium had a water volume of 300 L. 240 healthy juvenile fish were randomly selected, with an average weight of 52.35 ± 0.08) g, and a 12-week breeding experiment was conducted. The experiment was divided into 3 groups, with 4 replicates in each group, as shown below:

[0086] ① Algae oil protein composition 6 replacement group: fed with feed containing 25wt% fish meal + 25wt% algae oil protein composition 6;

[0087] ②Algae oil protein composition 7 replacement group: fed with feed containing 25wt% fish meal + 25wt% algae oil protein composition 7;

[0088] ③Algae oil protein composition 8 replacement group: fed with feed containing 25wt% fish meal + 25wt% algae oil protein composition 8.

[0089] The feed formula of the basic group was the same as that of Application Example 2. During the breeding experiment, the fish were fed twice a day (8:00 and 17:00). After the breeding, all the fish were starved for 24 hours, and then the number and weight of fish in each group were counted. After that, 6 fish were selected from each tank and quickly anesthetized with 100 mg / L MS-222, and their muscle tissues were taken for analysis of inosinic acid and protein content and texture indexes.

[0090] Table 4 Effects of different treatment groups of feed on growth and physiological and biochemical parameters of largemouth bass

[0091]

[0092] Note: The same lowercase letters in the same row indicate no significant difference

[0093] Figure 2 Table 4 shows the effects of feeds of different treatment groups in Application Example 4 on the meat texture indexes of largemouth bass. Table 4 shows the effects of feeds of different treatment groups in Application Example 4 on the growth and physiological and biochemical indexes of largemouth bass. Figure 2 It can be seen that the feed replaced by algae oil protein composition 8 significantly increased the weight gain rate, specific growth rate, muscle inosine and protein content, and meat hardness, adhesion, elasticity and chewiness of largemouth bass, but the feed conversion rate was significantly reduced. This shows that the feed replaced by algae oil protein composition 8 is better in improving fish growth, feed utilization, muscle inosine and protein content and meat quality.

[0094] The present invention combines the growth, feed utilization, inosinic acid and protein content and texture index results of relevant breeding experiments, confirming that the algae oil protein composition 8 replaces 50wt% of fish meal in feed to promote the growth of sea bass, improve feed utilization and muscle inosinic acid content, and improve the texture characteristics of fish meat, especially the elasticity and chewiness of fish meat. At the same time, the higher the inosinic acid content in fish meat, the lower the fishy smell of the meat and the more delicious the taste.

[0095] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. An algae oil protein composition for reducing the fishy smell and enhancing the freshness of largemouth bass meat, characterized in that: The algae oil protein composition comprises 2 to 10 parts of algae oil powder, 15 to 25 parts of porcine hemoglobin powder and 18 to 32 parts of dephenolized cottonseed protein; the algae oil powder is prepared by mixing cassava porous starch, sucrose fatty ester and DHA algae oil, dissolving in 50 to 90° C. distilled water, stirring, homogenizing under a pressure of 60 to 100 MPa to obtain a stable emulsion, and spray drying.

2. The algae oil protein composition for reducing the fishy smell and enhancing the freshness of largemouth bass meat according to claim 1, characterized in that: The algae oil protein composition comprises 5-9 parts of algae oil powder, 16-22 parts of pig hemoglobin powder and 20-29 parts of dephenolized cottonseed protein.

3. The algae oil protein composition for reducing the fishy smell and enhancing the freshness of largemouth bass meat according to claim 2, characterized in that: The algae oil protein composition comprises 7-8 parts of algae oil powder, 17-20 parts of pig hemoglobin powder and 25-28 parts of dephenolized cottonseed protein.

4. The algae oil protein composition for reducing the fishy smell and enhancing the freshness of largemouth bass meat according to claim 1, characterized in that: The concentration of cassava porous starch in the stable emulsion is 5-25wt%, the concentration of sucrose fatty ester in the stable emulsion is 10-35wt%, and the concentration of DHA algae oil in the stable emulsion is 5-22wt%.

5. The algae oil protein composition for reducing the fishy smell and enhancing the freshness of largemouth bass meat according to claim 1, characterized in that: The volume ratio of the total mass of the cassava porous starch, sucrose fatty ester and DHA algae oil to distilled water is (1-41) g: (50-500) mL.

6. The method for preparing the algae oil protein composition for reducing the fishy smell and increasing the freshness of the meat of largemouth bass according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1. Mix cassava porous starch, sucrose fatty ester and DHA algae oil, dissolve in 50-90°C distilled water and stir, homogenize at 60-100MPa to obtain a stable emulsion, spray dry the emulsion, and the inlet and outlet air temperatures are 120-160°C and 60-80°C, respectively, at a flow rate of 500-800mL / h to obtain algae oil powder; S2. The algae oil powder, which was crushed at 35-40° C. and passed through a 80-150 mesh sieve, the porcine hemoglobin powder and the dephenolized cottonseed protein were mixed evenly by a step-by-step mixing method to obtain an algae oil protein composition.

7. The method for preparing the algae oil protein composition for reducing the fishy smell and increasing the freshness of largemouth bass meat according to claim 6, characterized in that: The stirring speed in step S1 is 2000-4500 r / min, and the stirring time is 12-24 h.

8. The method for preparing the algae oil protein composition for reducing the fishy smell and increasing the freshness of largemouth bass meat according to claim 6, characterized in that: The homogenization time in step S1 is 40 to 120 minutes.

9. Use of the algae oil protein composition for reducing the fishy smell and increasing the freshness of largemouth bass meat as described in any one of claims 1 to 5 in the field of largemouth bass breeding.

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