Platinum nanoflower particles with uniform particle size as well as rapid synthesis method and application of platinum nanoflower particles

Abstract

The invention discloses platinum nanoflower particles with uniform particle size and a rapid synthesis method and application thereof. The synthesis method comprises the following steps: uniformly mixing a chloroplatinic acid solution with a reducing agent, and standing; adding a stabilizer into the solution obtained in the previous step, uniformly mixing, and reacting at 10-95 DEG C; and cleaning to obtain the platinum nanoflower particles. The method has the advantages that the repetition rate is high, a large number of platinum nanoflower particles with good morphology can be effectively obtained, raw materials are easy to obtain, process conditions are mild, and popularization of the platinum nanoflower particles is facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of nano material preparation, and in particular to a method for synthesizing metal nano flowers. Background Art

[0002] Nano-platinum (also known as platinum nanoflower) has good catalytic effect, dispersion effect, free radical removal effect, antioxidant effect, UV shielding effect, antibacterial effect, etc. Therefore, nano-platinum is widely used in many fields such as catalysts, pharmaceutical products, cosmetics, testing products, UV disinfection and sterilization equipment, etc. The preparation methods of nano-platinum are mainly divided into: solid phase method, gas phase method and liquid phase method. The solid phase method includes high-energy ball milling method, solid phase reaction method and large plastic deformation method; the gas phase method includes chemical vapor deposition, molecular beam epitaxy, resistance heating method, etc.; the liquid phase method includes precipitation method, microemulsion method, hydrothermal method, etc. The platinum nanoparticles prepared by these traditional chemical process methods are easy to precipitate and aggregate, and the morphology is difficult to control. In addition, the preparation process requires high-intensity instruments, raw materials and intermediates are easy to pollute the environment, the cost is high, and the preparation process is complicated. If a new synthesis process can be developed, the preparation process is simple, the operation requirements are not high, and nano-platinum with uniform morphology and particle size can be quickly obtained, the application of nano-platinum can be made wider and the application prospects are better. Summary of the invention

[0003] The purpose of the present invention is to provide a method for rapidly synthesizing platinum nanoflower particles with uniform particle size, so as to solve the problem of high requirements on the preparation conditions of nanoflowers in the prior art.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] A method for rapidly synthesizing platinum nanoflower particles with uniform particle size.

[0006] The steps include:

[0007] (1) mixing chloroplatinic acid solution and reducing agent evenly and allowing to stand until the solution is stable;

[0008] (2) Add a stabilizer to the solution obtained in the previous step, mix well, and then place the obtained solution at 10-95° C. for reaction, and wash to obtain platinum nanoflower particles.

[0009] Furthermore, the reducing agent is tripropylamine buffer.

[0010] Furthermore, the process of step (1) comprises: adding 100-500 μl of 10-50 nM chloroplatinic acid solution and 150-500 μl of 100-400 mmol / L tripropylamine buffer into 1000 μl of water, mixing evenly at room temperature, and standing until the solution is stable.

[0011] Furthermore, in step (1), the chloroplatinic acid solution and the reducing agent are mixed evenly and then allowed to stand for 2-5 minutes.

[0012] Furthermore, the stabilizer is one or more of polyvinyl pyrrolidone solution, ascorbic acid, sodium citrate, thiol compounds, polyethylene glycol, dendrimers, bovine serum albumin, dextran, chitosan, and ionic liquids.

[0013] Furthermore, the process of step (2) includes: adding 10-40 μl of 1%-20% polyvinyl pyrrolidone solution and 10-80 μl of 50-200 mg / ml ascorbic acid to the solution obtained in the previous step and mixing them evenly, reacting the obtained mixed solution at 10-95° C., and washing to obtain platinum nanoflower particles.

[0014] Furthermore, in step (2), the mixed solution is reacted in a water bath at 10-95° C. for 20-50 min.

[0015] The invention also provides platinum nanoflower particles with uniform particle size obtained by the synthesis method.

[0016] The present invention also provides an application of the platinum nanoflower particles with uniform particle size in the preparation of biomedical markers, drug delivery carriers and sensors.

[0017] The present invention also provides an application of the platinum nanoflower particles with uniform particle size in the preparation of biomacromolecule-related drugs.

[0018] The advantages of the present invention are: tripropylamine is used as a reducing agent, and the raw material chloroplatinic acid only needs to be simply mixed with the reducing agent and the stabilizer, and the platinum nanoflower particles can be synthesized in a short time of 20-50 minutes by controlling the reaction temperature in a water bath. The reducing agent tripropylamine buffer used is chemically stable at room temperature and is convenient for transportation and storage. The shortcomings of the existing platinum nanoflower particle synthesis steps, long synthesis time, high cost, and high operating requirements are solved. The repetition rate is high, and a large number of platinum nanoflower particles with good morphology can be effectively obtained, and the raw materials are easy to obtain, and the process conditions are mild, which is conducive to the promotion of platinum nanoflower particles. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The drawings described herein are used to provide a further understanding of the present invention, constitute a part of the present application, and do not constitute an improper limitation of the present invention. In the drawings:

[0020] Figure 1 It is a synthetic method flow chart of Example 1;

[0021] Figure 2 is the potential diagram of platinum nanoflower particles (PtNPs) obtained in Example 1;

[0022] Figure 3 is a transmission electron micrograph of platinum nanoflower particles (PtNPs) obtained in Example 1;

[0023] Figure 4 This is an experimental diagram of Example 4 verifying that platinum nanoflower particles (PtNPs) have peroxidase-like activity;

[0024] Figure 5-6 This is Example 5 about the combination of Pt@SANPs and SA antibody and the use of peroxidase-like activity to enhance the color development. DETAILED DESCRIPTION

[0025] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. The exemplary embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

[0026] Example 1

[0027] like Figure 1 As shown, this embodiment provides a method for rapidly synthesizing platinum nanoflower particles with uniform particle size, the steps comprising:

[0028] (1) Add 250 μl of 40 nM chloroplatinic acid solution and 250 μl of 200 mmol / L tripropylamine buffer to 1000 μl of water and mix well at room temperature;

[0029] (2) Add 40 μl of 15% polyvinyl pyrrolidone solution and 60 μl of 150 mg / ml ascorbic acid to the solution obtained in step (1), mix well, and then react the obtained mixed solution in a 60° C. water bath for 30 min, and wash to obtain platinum nanoflower particles (PtNPs).

[0030] The potential diagram of PtNPs obtained in this example is shown in Figure 2 As shown in FIG. 1 , it can be seen that the potential of these PtNPs is about -43 mV. The transmission electron microscopy photo of the PtNPs obtained in this example is shown in FIG. Figure 3 As shown, the particle size of these PtNPs is about 20-30 nm.

[0031] Example 2

[0032] This embodiment provides a method for rapidly synthesizing platinum nanoflower particles with uniform particle size, the steps comprising:

[0033] (1) 150 μl of 50 nM chloroplatinic acid solution and 200 μl of 150 mmol / L tripropylamine buffer were mixed at room temperature and allowed to stand until the solution was stable;

[0034] (2) Add 20 μl of 20% polyvinyl pyrrolidone solution and 50 mg / ml ascorbic acid to the solution obtained in step (1), mix well, and then react the resulting mixed solution in a 50° C. water bath for 20 min.

[0035] The potential and particle size of the PtNPs obtained in this example are comparable to those of the PtNPs in Example 1.

[0036] Example 3

[0037] This embodiment provides a method for rapidly synthesizing platinum nanoflower particles with uniform particle size, the steps comprising:

[0038] (1) 400 μl of 10 nM chloroplatinic acid solution and 200 μl of 300 mmol / L tripropylamine buffer were mixed at room temperature;

[0039] (2) Add 1% polyvinyl pyrrolidone solution and 20 μl of 200 mg / ml ascorbic acid to the solution obtained in step (1), mix well, and then react the resulting mixed solution in a 60° C. water bath for 50 min.

[0040] The potential and particle size of the PtNPs obtained in this example are comparable to those of the PtNPs in Example 1.

[0041] Example 4

[0042] This embodiment uses a typical H 2 O 2 -TMB system was used to study the peroxidase-like activity of PtNPs. Two experimental groups were set up, one with TMB, H 2 O 2 , 1 experimental group has H 2 O 2 , TMB, PtNPs, the results are as follows Figure 4 As shown in the figure, the results of the above two experimental groups correspond to samples No. 1 and No. 2 in the figure. 2 O 2 In the presence of PtNPs, TMB was rapidly oxidized to a blue product (OX-TMB). In the absence of PtNPs, TMB and H 2 O 2 The reaction solution was colorless, which indicated that PtNPs had peroxidase-like activity.

[0043] Experimental Example 5

[0044] The protrusions on the surface of PtNPs make it easy to modify biomacromolecules. In this experiment, streptavidin was modified on the surface of PtNPs. The process is as follows:

[0045] First, 20 μl PBS and 20 μl 1 mg / ml streptavidin (SA) were added to 200 μl PtNPs at the same time and vortexed for 2 hours; then, 1% BSA solution was added and incubated for 1 hour to block the unbound sites on the material; finally, the solution was centrifuged to remove the unbound SA. The precipitate was collected and resuspended in PBST (PBS containing 0.05% Tween-20) to obtain a Pt@SANPs solution. The Pt@SANPs solution was added dropwise to a test strip marked with SA antibody strips. When Pt@SANPs flowed through the SA antibody strips, Figure 5 , 6 As shown, No. 1 is a test strip with SA antibody strips, and No. 2 is a photo of Pt@SANPs binding to SA antibodies and developing color after flowing through the SA antibody strips, indicating that Pt@SANPs can be used as a signal material for test strips.

[0046] Add the peroxidase substrate 3-amino-9-ethylcarbazole (AEC) to the test strip. Figure 5 , 6 As shown in the photo No. 3, the gray strip turns reddish brown and the color is enhanced, indicating that Pt@SANPs can be used to enhance the sensitivity of the test strip.

[0047] The PtNPs synthesis process provided by the present invention solves the shortcomings of the existing PtNPs synthesis process, which is complicated, takes a long time and has high cost. It is simple and easy to operate. The PtNPs synthesis method provided by the present invention can synthesize PtNPs in a short time of only about 20-50 minutes.

[0048] The high repetition rate can effectively obtain a large number of PtNPs with good morphology and continuously adjustable size. Moreover, the raw materials are easily available and can be widely promoted.

[0049] The technical solutions provided by the embodiments of the present invention are introduced in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the embodiments of the present invention. The description of the above embodiments is only applicable to help understand the principles of the embodiments of the present invention. At the same time, for those skilled in the art, according to the embodiments of the present invention, there will be changes in the specific implementation methods and application scopes. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A method for rapidly synthesizing platinum nanoflower particles with uniform particle size, characterized in that: The steps include: (1) mixing chloroplatinic acid solution and reducing agent evenly and allowing to stand until the solution is stable; (2) Add a stabilizer to the solution obtained in the previous step, mix well, and then place the obtained solution at 10-95° C. for reaction, and wash to obtain platinum nanoflower particles.

2. The method for rapidly synthesizing platinum nanoflower particles with uniform particle size according to claim 1, characterized in that: The reducing agent is tripropylamine buffer.

3. A method for rapidly synthesizing platinum nanoflower particles with uniform particle size according to claim 1 or 2, characterized in that: The process of step (1) comprises: adding 100-500 μl of 10-50 nM chloroplatinic acid solution and 150-500 μl of 100-400 mmol / L tripropylamine buffer into 1000 μl of water, mixing evenly at room temperature, and standing until the solution is stable.

4. A method for rapidly synthesizing platinum nanoflower particles with uniform particle size according to claim 1 or 2, characterized in that: In the step (1), the chloroplatinic acid solution and the reducing agent are mixed evenly and then allowed to stand for 2-5 minutes.

5. The method for rapidly synthesizing platinum nanoflower particles with uniform particle size according to claim 1, characterized in that: The stabilizer is one or more than two of polyvinyl pyrrolidone solution, ascorbic acid, sodium citrate, thiol compounds, polyethylene glycol, dendritic polymer, bovine serum albumin, dextran, chitosan and ionic liquid.

6. A method for rapidly synthesizing platinum nanoflower particles with uniform particle size according to claim 1 or 5, characterized in that: The process of step (2) comprises: adding 10-40 μl of 1%-20% polyvinyl pyrrolidone solution and 10-80 μl of 50-200 mg / ml ascorbic acid to the solution obtained in the previous step and mixing them evenly, reacting the obtained mixed solution at 10-95° C., and washing to obtain platinum nanoflower particles.

7. The method for rapidly synthesizing platinum nanoflower particles with uniform particle size according to claim 1 or 5, characterized in that: The mixed solution in step (2) is reacted in a water bath at 10-95° C. for 20-50 min.

8. Platinum nanoflower particles with uniform particle size obtained by the synthesis method as described in any one of claims 1 to 7.

9. Use of the platinum nanoflower particles with uniform particle size as claimed in claim 8 in the preparation of biomedical markers, drug delivery carriers, and sensors.

10. Use of the platinum nanoflower particles with uniform particle size as claimed in claim 8 in the preparation of biomacromolecule-related drugs.

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