Constant-turbidity continuous culture system and constant-turbidity continuous culture method

Abstract

The invention provides a constant-turbidity continuous culture system which comprises a photoreactor used for containing a culture solution; the peristaltic pump array comprises a plurality of peristaltic pumps, part of the peristaltic pumps are used for pumping nutritional ingredients into the photoreactor, and the other part of the peristaltic pumps are used for pumping out a culture solution in the photoreactor; the sensor group is positioned in the photoreactor and is used for continuously monitoring the environmental information of the growth of the photosynthetic organisms in the culture solution; and the controller is electrically connected with the plurality of peristaltic pumps and the sensor group and is used for driving the plurality of peristaltic pumps to work according to the environment information. The invention further provides a constant-turbidity continuous culture method.

Description

Technical Field

[0001] The present application relates to the technical field of photosynthetic organism cultivation, and in particular to a turbidostatic continuous cultivation system and a turbidostatic continuous cultivation method for photosynthetic organisms. Background Art

[0002] Traditional large-scale cultivation methods for microalgae use batch culture, which means that after inoculation, they continue to grow to a preset concentration and then are harvested in batches.

[0003] The above batch culture method has the following main problems: after each inoculation, it is necessary to start growing from a low concentration to a preset concentration before harvesting, which requires a delay period, a logarithmic period, and a stable period before reaching the preset concentration, resulting in a waste of production time; the culture system needs to be emptied after each harvest, which easily introduces pollutants and reduces the service life of the photoreactor; each harvest is large, and a large container is required as a buffer, which wastes workshop space and is not conducive to assembly line production. Summary of the invention

[0004] The first aspect of the present application provides a constant turbidity continuous culture system, comprising: a photoreactor for containing a culture solution; a peristaltic pump array, comprising a plurality of peristaltic pumps, some of which are used to pump nutrients into the photoreactor, and another portion of which is used to pump out the culture solution in the photoreactor; a sensor group, located in the photoreactor, for continuously monitoring environmental information of the growth of photosynthetic organisms in the culture solution; and a controller, electrically connected to the plurality of peristaltic pumps and the sensor group, respectively, for driving the plurality of peristaltic pumps to work according to the environmental information.

[0005] The second aspect of the present application provides a constant turbidity continuous culture method, which is applied to the above-mentioned constant turbidity continuous culture system; the constant turbidity continuous culture method includes: monitoring the environmental information of the growth of photosynthetic organisms in the culture solution in the photoreactor; according to the environmental information, controlling part of the peristaltic pump to pump nutrients into the photoreactor, and controlling another part of the peristaltic pump to pump out the culture solution in the photoreactor.

[0006] The above-mentioned turbidity-constant culture system and turbidity-constant culture method of the present application of the embodiment of the present application monitor the environmental information of the growth of photosynthetic organisms in real time through the sensor group, and feedback control the peristaltic pump array to pump in the culture medium, pH adjustment components, other special components and pump out the culture solution. In this way, the turbidity-constant culture system can continuously harvest photosynthetic organisms on the basis of maintaining a better culture state. Further, the above-mentioned feedback control process maintains the growth of photosynthetic organisms within a preset concentration range, and the preset concentration range is based on the maximum yield concentration under the culture medium, light, temperature, and aeration conditions provided by the turbidity-constant culture system, to achieve high efficiency (because continuous production is maintained at the highest yield concentration), low cost (no need to waste a lot of electricity to carry out meaningless delay period and logarithmic period growth), and simple maintenance (no need for a lot of manpower participation every time harvesting) production. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] Figure 1 2 is a system architecture diagram of a turbidostatic continuous culture system according to an embodiment of the present application.

[0008] Figure 2 Flow chart of the turbidostatic continuous culture method according to an embodiment of the present application.

[0009] Main component symbols Constant turbidity continuous culture system: 100; Photoreactor: 1; Sensor Group: 2; Raman sensor: 21; pH sensor: 22; Temperature sensor: 23; Dissolved oxygen sensor: 24; Salinity sensor: 25; Peristaltic pump array: 3; Peristaltic pump: 31; Culture condition control components: 4; Lighting control device: 41; Water cooling control device: 42; Aeration control device: 43; Controllers: 5; Assembly shell: 6; Interfaces: 61; Display: 7; Antenna: 8; Culture medium:200.

[0010] The following specific implementation methods will further illustrate the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0011] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all of the embodiments.

[0012] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0013] In order to further explain the technical means and effects adopted by the present application to achieve the intended purpose, the present application is described in detail below in conjunction with the accompanying drawings and preferred implementation methods.

[0014] Photosynthetic organisms (such as microalgae, duckweed, etc.) have the ability of photosynthesis and can be widely used in the fields of medicine, energy, environment, food, etc. The present application provides a constant turbidity continuous culture system, which can continuously culture and harvest photosynthetic organisms while maintaining the highest growth efficiency of photosynthetic organisms by real-time monitoring of the environmental information of photosynthetic organisms, the growth information of photosynthetic organisms, and the state information of the culture solution, and feedback control of the operation of each module. The constant turbidity continuous culture system can be applied to both laboratory culture scenarios and industrial culture scenarios.

[0015] See also Figure 1 The turbidostatic continuous culture system 100 of the embodiment of the present application includes a photoreactor 1, a sensor group 2, a peristaltic pump array 3, a culture condition control component 4 and a controller 5.

[0016] The photoreactor 1 is used to hold the culture solution 200 of photosynthetic organisms for the growth of photosynthetic organisms. The culture solution 200 referred to in this embodiment includes the whole material contained in the photoreactor 1, such as photosynthetic biological cells, water, and culture medium. The sensor group 2 is located in the photoreactor 1 and is directly immersed in the culture solution 200, and is used to monitor the environmental information of the growth of photosynthetic organisms, the growth information of photosynthetic organisms, and the status information of the culture solution in real time. The peristaltic pump array 3 is connected to the photoreactor 1 and the external container, and is used to pump various growth components into the photoreactor 1 or to pump out the culture solution in the photoreactor 1. The culture condition control component 4 is used to provide the culture conditions (such as light, temperature, etc.) required for the growth of photosynthetic organisms. The controller 5 is electrically connected to the sensor group 2, the peristaltic pump array 3, and the culture condition control component 4, respectively, and is used to feedback control the peristaltic pump array 3 and the culture condition control component 4 according to the environmental information, growth information, and status information transmitted back by the sensor group 2.

[0017] The sensor group 2 includes a plurality of sensors that work independently and have different functions. In this embodiment, the sensor group 2 includes five sensors, namely a Raman sensor 21, a pH sensor 22, a temperature sensor 23, a dissolved oxygen sensor 24, and a salinity sensor 25. During the operation of the turbidostatic continuous culture system 100, the Raman sensor 21, the pH sensor 22, the temperature sensor 23, the dissolved oxygen sensor 24, and the salinity sensor 25 are all immersed in the culture solution 200.

[0018] The Raman sensor 21 is used to monitor the environmental information of the photosynthetic organisms growing in the culture solution 200 and the growth information of the photosynthetic organisms in real time. The environmental information includes the content of each nutrient salt in the culture medium in the culture solution 200, and the growth information includes the cell dry weight, pigment content, cell wall component content, cell membrane and other component content of the photosynthetic organisms. The light source of the Raman sensor 21 emits reference light (laser) to the culture solution 200, and obtains the above-mentioned environmental information and growth information based on the received detection light spectrum. The wavelength selection of the reference light needs to consider the fluorescence interference of the photosynthetic organisms on the one hand, and the penetration depth of the reference light itself on the other hand.

[0019] For fluorescence interference: Using a shorter wavelength laser (such as an ultraviolet laser) may excite the fluorescence of the sample, thereby interfering with the Raman signal. Long-wavelength lasers (such as near-infrared lasers) can usually reduce the autofluorescence of the sample, which is particularly important for samples with strong fluorescence backgrounds. In this embodiment, photosynthetic organisms are microalgae as an example, and the main pigments in microalgae cells include chlorophyll a and chlorophyll b. The fluorescence emission peak of chlorophyll a is usually around 685 nanometers, which is the typical fluorescence emission wavelength of chlorophyll a, but the specific value may vary slightly due to factors such as the type of microalgae and environmental conditions. The fluorescence emission peak of chlorophyll b is usually around 730 nanometers, but its content is usually less than that of chlorophyll a, and the fluorescence intensity is also lower. In addition, microalgae may also contain a small amount of other pigments besides chlorophyll a and chlorophyll b, such as carotenoids (including carotene and lutein), whose fluorescence emission peaks are usually at longer wavelengths, such as in the range of 500-550 nanometers. Therefore, in this embodiment, the main fluorescence interference wavelengths are about 685 nanometers, about 730 nanometers, and 500-550 nanometers.

[0020] Regarding penetration depth: the longer the laser wavelength, the stronger its penetration in the sample. Therefore, in this embodiment, using a laser with a longer wavelength to detect the environmental information and growth information of the algae liquid is conducive to obtaining more accurate results.

[0021] In at least one embodiment of the present application, the reference light emitted by the Raman sensor 21 is a laser with a wavelength of 785 nanometers. Laser with this wavelength can reduce the fluorescence background of various samples and is suitable for detection and analysis of biological samples and certain organic compounds.

[0022] In at least one embodiment of the present application, the reference light emitted by the Raman sensor 21 is a laser with a wavelength of 1064 nanometers. Laser with this wavelength can be used for deep tissue penetration or to reduce fluorescence interference.

[0023] In this embodiment, the pH sensor 22, the temperature sensor 23, the dissolved oxygen sensor 24 and the salinity sensor 25 are used to monitor the status information of the culture solution 200 in real time. The pH sensor 22 is used to monitor the pH value of the culture solution 200 in real time. The temperature sensor 23 is used to monitor the temperature value of the culture solution 200 in real time. The dissolved oxygen sensor 24 is used to monitor the dissolved oxygen content of the culture solution 200 in real time. The salinity sensor 25 is used to monitor the salinity value of the culture solution 200 in real time. That is, the above-mentioned status information includes the pH value, temperature value, dissolved oxygen content and salinity value of the culture solution 200.

[0024] The peristaltic pump array 3 includes multiple peristaltic pumps 31, some of which are used to pump culture medium into the photoreactor 1, some of which are used to pump pH adjusting components into the reactor 1, some of which are used to pump special components for specific types of photosynthetic organisms into the photoreactor 1, and another part of the peristaltic pumps 31 is used to pump out culture medium to harvest photosynthetic organisms when the photosynthetic organisms grow to a preset state, or to pump out a small amount of culture medium for sampling and observation during the culture process.

[0025] In this embodiment, the culture medium includes the following nutrients 1-10 (all in solution).

[0026] 1. Nitrogen (N), common form: nitrate ( )、ammonium( ), urea; Nitrogen is essential for protein synthesis and cell growth.

[0027] 2. Phosphorus (P), common form: phosphate ( ). Phosphorus is essential for energy transfer (ATP) and nucleic acid synthesis.

[0028] 3. Potassium (K), which is essential for enzyme activation and osmotic regulation.

[0029] 4. Magnesium (Mg), the central component of the chlorophyll molecule, is important for photosynthesis and enzyme function.

[0030] 5. Calcium (Ca), which is important for cell wall stability and signal transduction.

[0031] 6. Sulfur (S), certain amino acids (cysteine ​​and methionine) and vitamins.

[0032] 7. Iron (Fe), essential for electron transport in photosynthesis and respiration.

[0033] 8. Trace elements (trace nutrients, mixed solutions), including: Manganese (Mn), an enzyme cofactor in photosynthesis; Zinc (Zn), enzyme activation and protein synthesis; Copper (Cu), electron transport in photosynthesis and respiration; Molybdenum (Mo), nitrogen assimilation; Cobalt (Co), synthesis of vitamin B12; Boron (B), cell wall formation and stability.

[0034] 9. Carbon (C) (optional) if not using carbon dioxide ( ) as a carbon source, bicarbonate ( ) in the form of carbon. Carbon is the backbone of all organic molecules and is essential for photosynthesis.

[0035] 10. Vitamins (optional), some algae require specific vitamins (e.g., B12, thiamine) for optimal growth.

[0036] In this embodiment, the pH adjusting component includes: 11. Buffer, add buffer, such as sodium bicarbonate ( ) or potassium bicarbonate ( ) to stabilize pH. Buffers help resist changes in pH by neutralizing acids or bases; Adjusting buffer concentration: Determine the optimal concentration of buffer for a specific algae species and culture conditions; 12. Acid (to lower pH) by adding a small controlled amount of dilute acid, such as hydrochloric acid (HCl) or sulfuric acid ( ); 13. Alkali (to raise pH) by adding a dilute base such as sodium hydroxide (NaOH) or potassium hydroxide (KOH) in small, controlled amounts.

[0037] In this embodiment, the above-mentioned other special components mainly include: 14. Growth factors (optional) and hormones (optional), sometimes plant hormones such as gibberellins, cytokinins, etc. are added; 15. Buffer (optional): used to maintain the pH value of the culture medium, such as HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic; 16. Antibiotics (optional), used to inhibit the growth of bacteria and other microorganisms, such as streptomycin, penicillin, etc.; 17. Concentrated sea salt (optional when cultivating seaweed). When replenishing the mother solution, if the concentration of the mother solution is low, the salinity of the culture solution may decrease. Appropriate supplementation of sea salt can maintain the salinity and osmotic pressure. 18. Pure water (optional when cultivating seaweed). Aeration will take away water and may increase salinity. Adding pure water can moderately dilute and maintain salinity and osmotic pressure. 19. Defoamer (optional). If the amount of organic matter increases during the culture process, the surface tension of the liquid may become stronger, and a large number of bubbles will exist on the liquid surface. The generation of foam may cause a series of problems, such as overflow, reduced oxygen transfer efficiency, reduced stirring efficiency, and damaged product quality. Defoamer molecules have low surface tension, and they can quickly diffuse to the surface of the foam, destroying the stability of the foam. 20. Heavy metal chelators (optional), such as EDTA, used to chelate heavy metal ions that may be toxic to cells; 21. Antioxidants (optional), such as ascorbic acid (vitamin C), α-tocopherol (vitamin E), etc., are used to protect cells from oxidative stress; 22. Toxins (optional) and Inhibitors (optional). In some experiments, you may need to add specific toxins or inhibitors to study the response of cells to stress. For example, Haematococcus pluvialis needs to be under stress to produce astaxanthin.

[0038] In this embodiment, one of the peristaltic pumps 31 is used to pump out the culture solution in the photoreactor 1 to harvest photosynthetic organisms, and one of the peristaltic pumps 31 is used to pump out a small amount of culture solution in the photoreactor 1 for sampling and observation. Each of the above-mentioned culture medium, pH adjustment components and other special components is pumped into the photoreactor 1 by a peristaltic pump 31, so 24 peristaltic pumps 31 are required in this embodiment.

[0039] The culture condition control assembly 4 includes a light control device 41, a water cooling control device 42 and an aeration control device 43. The light control device 41, the water cooling control device 42 and the aeration control device 43 are electrically connected to the controller 5 and work independently of each other. The light control device 41 is used to control the light intensity and light quality during the culture process to provide light conditions suitable for the growth of photosynthetic organisms. The water cooling control device 42 is used to control the temperature of the culture solution 200. The aeration control device 42 is used to control the amount of carbon dioxide pumped into the culture solution 200.

[0040] In this embodiment, the aeration control device 43 can be based on The pH value of the culture solution 200 can be adjusted according to the supply ratio of the microalgae. The pH value of the culture solution 200 can also be adjusted according to the growth stage of the microalgae. The faster the growth, the higher the pH value. The more there is, the higher the pH will be.

[0041] In at least one embodiment of the present application, the following method is used to adjust Pumping amount: The real-time pH value of the culture liquid 200 and the number of operations of the peristaltic pump 31 that pumps out the algae liquid are monitored by the pH sensor 22. If the pH value deviates from the target range, the amount of acid or alkali that needs to be supplemented is calculated based on the degree of deviation and the number of operations of the discharge pump (reflecting the growth rate). The specific operation is: when the number of times the peristaltic pump pumps out decreases and the pH drops (growth slows down), or the pH value is lower than the lower limit of the above target range, the controller 5 calculates the amount of alkali that needs to be supplemented, and controls the aeration control device 43 to appropriately reduce the amount of alkali. Supply; when the peristaltic pump 31 pumps out more times and the pH rises (growth becomes faster), or when the pH value is higher than the upper limit of the target range, the controller 5 calculates the amount of acid required to be added and controls the aeration control device 43 to increase supply.

[0042] The amount of supplement should be adjusted according to the pH deviation and the number of times the peristaltic pump 31 runs to ensure that the pH value is stable within the target range and meet the growth requirements of photosynthetic organisms. Only when the deviation is large, it is necessary to add acid or alkali to adjust the pH value. In some embodiments, the buffer is coupled with the operation of the peristaltic pump 31 to maintain a certain concentration of the buffer to prevent the pH value of the culture solution 200 from fluctuating too much.

[0043] The controller 5 is a circuit board, including various electronic components such as computing chips, and is used to receive information fed back by each sensor in the sensor group 2, determine the working mode of the peristaltic pump array 3 and the culture condition control component 4 based on a preset feedback control algorithm, and drive the peristaltic pump array 3 and the culture condition control component 4 to work, so that during the cultivation of photosynthetic organisms, the culture solution 200 can be maintained in a state where the photosynthetic organisms can grow efficiently, which is conducive to continuous and efficient harvesting of photosynthetic organisms.

[0044] In the present embodiment, the constant turbidity continuous culture system 100 also includes an assembly shell 6. The assembly shell 6 as a whole is roughly a hollow rectangular parallelepiped structure. 24 peristaltic pumps 31 are arranged in an array including two rows and twelve columns. The 24 peristaltic pumps 31 are embedded in the same surface of the assembly shell 6. An interface connected to the sensor group 2 is provided on the surface of the assembly shell 6 on which the peristaltic pumps 31 are embedded. In the present embodiment, five interfaces 61 are provided on the assembly shell 6, and the five interfaces 61 are connected one-to-one with the five sensors in the sensor group 2. The controller 5 is located inside the assembly shell 6, and is electrically connected to the 24 peristaltic pumps, the five interfaces 61 and the culture condition control component 4 respectively. In the present embodiment, by providing the assembly shell 6, the overall integration of the system is made higher.

[0045] In this embodiment, the turbidity-controlled continuous culture system 100 further includes a display screen 7 and an antenna 8. The display screen 7 and the peristaltic pump 31 are embedded on the same surface of the assembly shell 6, and the antenna is fixedly connected to the other surface of the assembly shell 6. The display screen 7 and the antenna 8 are electrically connected to the controller 5, respectively. The display screen 7 is used to display various types of information (such as the aforementioned environmental information, growth information, and status information) of the turbidity-controlled continuous culture system 100 during operation. The antenna can be used to transmit data generated by the turbidity-controlled continuous culture system 100 to an external terminal device (such as an intelligent portable terminal, a host).

[0046] The embodiment of the present application also provides a turbidostatic continuous culture method, which is applied to the above-mentioned turbidostatic continuous culture system 100 .

[0047] See also Figure 2 The constant turbidity continuous culture method of this embodiment includes: Step S1, real-time monitoring of environmental information of photosynthetic organism growth and real-time monitoring of status information of culture solution.

[0048] In this embodiment, step S1 includes: real-time monitoring of environmental information of photosynthetic organism growth through a Raman sensor 21. The environmental information includes the content of each nutrient salt in the culture medium in the culture solution. In this embodiment, step S1 also includes: real-time monitoring of the pH value of the culture solution through a pH sensor 22, real-time monitoring of the temperature value of the culture solution through a temperature sensor 23, real-time monitoring of the dissolved oxygen content of the culture solution through a dissolved oxygen sensor 24, and monitoring of the salinity of the culture solution through a salinity sensor 25. That is, the status information includes the pH value, temperature value, dissolved oxygen content and salinity value of the culture solution. In other embodiments of the present application, the status information may include one or any combination of the pH value, temperature value, dissolved oxygen content and salinity value of the culture solution.

[0049] In at least one embodiment of the present application, step S1 further includes: real-time monitoring of growth information of photosynthetic organisms by means of Raman sensor 21. The growth information includes cell dry weight, pigment content, cell wall component content, cell membrane component content, etc. of photosynthetic organisms.

[0050] Step S2, controlling part of the peristaltic pump to pump culture medium into the photoreactor, and controlling part of the peristaltic pump to pump out culture medium in the photoreactor according to the environmental information, the dissolved oxygen content and the salinity value; Step S3, according to the pH value control part, the peristaltic pump pumps the pH value adjusting component into the photoreactor; and / or, according to the pH value, the aeration control device controls the aeration control device to pump carbon dioxide into the photoreactor; Step S4, controlling the water cooling control device to cool the culture solution according to the temperature value; Step S5: driving the lighting control device to control light intensity and light quality according to the environmental information and the state information.

[0051] In this embodiment, after the step of controlling the peristaltic pump to pump out the culture solution in the photoreactor, step S6 is further included to harvest the photosynthetic organisms and extract the target product.

[0052] In at least one embodiment of the present application, the controller 5 predicts the cell dry weight of photosynthetic organisms based on the Raman spectrum obtained by the Raman sensor 21, and predicts the content of each nutrient salt in the culture medium in the culture solution 200 in combination with a deep learning algorithm, so as to control the operation of each peristaltic pump 31 and supplement the content of different components, so that the content of each nutrient salt in the culture medium is maintained at an ideal value (the value that maximizes the growth efficiency of photosynthetic organisms).

[0053] In other modified embodiments of the present application, the operation of each functional module in the turbidostatic continuous culture system 100 can also be controlled by an external control device other than the controller 5. For example, the concentration of the input culture medium is adjusted by a proportional control valve (a peristaltic pump 31 pumps in a more concentrated culture medium, and a peristaltic pump 31 pumps in pure water to adjust the concentration of the input culture medium by dilution in proportion).

[0054] The above-mentioned turbidity-constant culture system 100 and turbidity-constant culture method of the present application monitors the environmental information of photosynthetic organisms, the growth information of photosynthetic organisms and the state information of the culture solution in real time through the sensor group 2 (including Raman sensor 21, pH sensor 22, temperature sensor 23, dissolved oxygen sensor 24 and salinity sensor 25), and feedback controls the peristaltic pump array 3 to pump in the culture medium, pH value adjustment components, other special components and pump out the culture solution, and feedback controls the culture condition control component 4 (including the light control device 41, the water cooling control device 42 and the aeration control device 43) to work. In this way, the turbidity-constant culture system 100 can continuously harvest photosynthetic organisms on the basis of maintaining a relatively optimal culture state.

[0055] Taking the photosynthetic organism P. triangularis as an example, the peristaltic pump 31 is controlled to pump out the algae liquid, and the peristaltic pump 31 is controlled to add culture medium according to the ratio (content) information of the nutrient salts in the culture liquid sent back in real time by the Raman sensor 21 (the amount of each component in the culture medium added is determined according to the information sent back by the Raman sensor 21), so that the growth of P. triangularis is maintained within a preset concentration range. The preset concentration range is based on the maximum yield concentration of fucoxanthin under the culture medium, light, temperature, and aeration conditions provided by the turbidity-constant continuous culture system 100, so as to achieve high efficiency (because continuous production is maintained at the highest yield concentration), low cost (no need to waste a lot of electricity for meaningless delay period and logarithmic period growth), and simple maintenance (no need for a lot of manpower participation each time harvesting) of fucoxanthin production.

[0056] A traditional continuous culture mode is mostly based on the principle of chemostat culture. This culture method limits the precise control of culture medium components to a certain extent, and thus may not always ensure that photosynthetic organisms (such as microalgae) are in the optimal growth state. Raman sensor is a detection technology based on the principle of Raman scattering, which can provide information about the molecular vibration of a substance. In the field of biotechnology, Raman spectroscopy can be used to analyze biological samples, such as the chemical composition of microalgae, culture medium components, etc. The embodiment of the present application predicts the components in the culture medium by using Raman sensors combined with deep learning algorithms, and realizes stable continuous culture through a multi-channel precise control system.

[0057] Those skilled in the art should recognize that the above embodiments are only used to illustrate the present application and are not intended to be limiting of the present application. As long as they are within the spirit and scope of the present application, appropriate changes and modifications to the above embodiments are within the scope of protection claimed in the present application.

Claims

1. A turbidostatic continuous culture system, characterized in that: include: A photoreactor for containing a culture solution; A peristaltic pump array, comprising a plurality of peristaltic pumps, some of which are used to pump nutrients into the photoreactor, and another portion of which are used to pump out the culture solution in the photoreactor; A sensor group, located in the photoreactor, for continuously monitoring environmental information of the growth of photosynthetic organisms in the culture solution; as well as The controller is electrically connected to the multiple peristaltic pumps and the sensor group, and is used to drive the multiple peristaltic pumps to work according to the environmental information.

2. The turbidostatic continuous culture system according to claim 1, characterized in that: The sensor group is also used to monitor the status information of the culture solution, and the constant turbidity continuous culture system also includes a culture condition control component; The controller is electrically connected to the culture condition control component, and is also used to control the operation of the culture condition control component according to the environmental information and the status information.

3. The turbidostatic continuous culture system according to claim 2, characterized in that: The sensor group includes a Raman sensor electrically connected to the controller, and the Raman sensor is used to continuously monitor the environmental information.

4. The turbidostatic continuous culture system according to claim 3, characterized in that: The Raman sensor is also used to continuously monitor the growth information of the photosynthetic organism.

5. The turbidostatic continuous culture system according to claim 4, characterized in that: The photosynthetic organism is microalgae, and the Raman sensor is used to emit a reference light of 785 nm or 1064 nm to the photosynthetic organism to detect the environmental information and the growth information; The environmental information includes the nutrient salt content of the culture medium in the culture solution, and the growth information includes one or any combination of the cell dry weight, pigment content, cell wall component content, and cell membrane of the photosynthetic organism.

6. The turbidostatic continuous culture system according to claim 2, characterized in that: The sensor group includes a pH sensor for monitoring the pH value of the culture solution; The nutritional ingredients include pH adjusting ingredients The culture condition control assembly includes an aeration control device; The controller is electrically connected to the pH sensor and the aeration control device, respectively, and is used to control the peristaltic pump to pump the pH adjusting component into the photoreactor according to the pH value, and / or to drive the aeration control device to control the amount of carbon dioxide pumped into the photoreactor according to the pH value.

7. The turbidostatic continuous culture system according to claim 2, characterized in that: The sensor group includes a temperature sensor for monitoring the temperature value of the culture solution; The culture condition control component includes a water cooling control device; The controller is electrically connected to the temperature sensor and the water cooling control device, respectively, and is used to control the water cooling control device to cool the culture solution according to the temperature value.

8. The turbidostatic continuous culture system according to claim 2, characterized in that: The sensor group includes a dissolved oxygen sensor and a salinity sensor which are electrically connected to the controller respectively. The dissolved oxygen sensor is used to monitor the dissolved oxygen value of the culture solution, and the salinity sensor is used to monitor the salinity value of the culture solution.

9. The turbidostatic continuous culture system according to claim 2, characterized in that: The culture condition control assembly also includes a lighting control device; The controller is electrically connected to the lighting control device, and is used to drive the lighting control device to control light intensity and light quality according to the environmental information and the state information.

10. The turbidostatic continuous culture system according to any one of claims 1 to 9, characterized in that: Part of the peristaltic pump is used to pump culture medium into the photoreactor, part of the peristaltic pump is used to pump pH adjustment components into the photoreactor, and part of the peristaltic pump is used to pump special components into the photoreactor, wherein the special components include one or any combination of growth factors and hormones, buffers, antibiotics, concentrated sea salt, pure water, defoaming agents, heavy metal chelators, antioxidants, toxins and inhibitors.

11. The turbidostatic continuous culture system according to any one of claims 1 to 9, characterized in that: It also includes a hollow assembly shell, on which a plurality of interfaces are formed; Each of the peristaltic pumps is embedded in the same surface of the assembly shell, the controller is located inside the assembly shell, and the sensor group is connected to the multiple interfaces.

12. The turbidostatic continuous culture system according to claim 11, characterized in that: An antenna connected to the controller is also fixedly arranged on the assembly housing; The surface of the assembly housing where the peristaltic pump array is arranged is also provided with a display screen.

13. A turbidity-constant continuous culture method, characterized in that: Used in the turbidostatic continuous culture system as claimed in claim 1; The constant turbidity continuous culture method comprises: Monitoring the environmental information of the growth of photosynthetic organisms in the culture solution in the photoreactor; According to the environmental information, part of the peristaltic pump is controlled to pump nutrients into the photoreactor, and another part of the peristaltic pump is controlled to pump out the culture solution in the photoreactor.

14. The turbidostatic continuous culture method according to claim 13, characterized in that: The turbidostatic continuous culture system also includes a culture condition control component; The constant turbidity continuous culture method also includes: Monitoring the status information of the culture solution in the photoreactor; The culture condition control component is controlled to operate according to the environmental information and the status information.

15. The turbidostatic continuous culture method according to claim 14, characterized in that: The step of monitoring the environmental information of the growth of photosynthetic organisms in the culture solution in the photoreactor comprises: The environmental information of photosynthetic organisms in the culture solution in the photoreactor is monitored in real time by a Raman sensor.

16. The turbidostatic continuous culture method according to claim 15, characterized in that: The step of real-time monitoring of environmental information of photosynthetic organisms in the culture solution in the photoreactor by a Raman sensor comprises: The environmental information of the photosynthetic organisms in the culture solution in the photoreactor and the growth information of the photosynthetic organisms are monitored in real time by a Raman sensor.

17. The turbidostatic continuous culture method according to claim 16, characterized in that: The step of monitoring the environmental information of photosynthetic organisms in the culture solution in the photoreactor and the growth information of the photosynthetic organisms in real time by using a Raman sensor comprises: A Raman sensor is used to emit a reference light of 785 nm or 1064 nm to the photosynthetic organism to detect the environmental information and the growth information, wherein the environmental information includes the content of each nutrient salt in the culture medium in the culture solution, and the growth information includes one or any combination of the cell dry weight, pigment content, cell wall component content, and cell membrane of the photosynthetic organism.

18. The turbidostatic continuous culture method according to claim 14, characterized in that: The sensor group includes a pH sensor, the nutrient component includes a pH value adjusting component, and the culture condition control component includes an aeration control device; The step of controlling the operation of the culture condition control component according to the environment information and the state information comprises: The peristaltic pump is used to pump the pH adjusting component into the photoreactor according to the pH value control part, and / or is used to drive the aeration control device to control the amount of carbon dioxide pumped into the photoreactor according to the pH value.

19. The turbidostatic continuous culture method according to claim 14, characterized in that: The sensor group includes a temperature sensor, and the culture condition control component includes a water cooling control device; The step of controlling the operation of the culture condition control component according to the environment information and the state information comprises: The water cooling control device is controlled according to the temperature value to cool the culture solution.

20. The turbidostatic continuous culture method according to claim 14, characterized in that: The sensor group includes a dissolved oxygen sensor and a salinity sensor electrically connected to the controller respectively, and the step of monitoring the state information of the culture solution in the photoreactor includes: The dissolved oxygen value and salinity value of the culture solution in the photoreactor are monitored.

21. The turbidostatic continuous culture method according to claim 14, characterized in that: The culture condition control assembly also includes a lighting control device; The step of controlling the operation of the culture condition control component according to the environment information and the state information comprises: The lighting control device is driven according to the environmental information and the state information to control the light intensity and light quality.

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