Protease chromatography extraction device and extraction method
By designing a protease chromatography extraction device, the clamping assembly and adjustment assembly are used to ensure that the diversion glass rod comes into contact with the inner wall of the chromatography column, the problem of instability in solution addition is solved and the chromatography effect is improved.
Patent Information
- Application Number
- CN202411988676.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-04-15
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In the existing protease chromatography extraction methods, the solution is unstable, which easily produces bubbles, interferes with the interaction between protease and chromatography column fillers, and reduces the chromatography effect.
A protease chromatography extraction device is designed to fix the chromatography column by clamping the assembly, adjust the angle and length of the diversion glass rod to make it come into contact with the inner wall of the chromatography column, ensuring that the solution flows stably along the inner wall of the chromatography column.
The stable and uniform addition of the solution is achieved, which reduces the generation of bubbles, improves the interaction between protease and chromatography column filler, and significantly improves the chromatography effect.
Smart Images
Figure CN119818993A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of enzyme extraction, and in particular relates to a protease chromatography extraction device and an extraction method. Background Art
[0002] Protease is a general term for a class of enzymes that hydrolyze protein peptide chains. According to the way they degrade polypeptides, they are divided into two categories: endopeptidases and terminal peptidases. The former can cut large molecular weight polypeptide chains from the middle to form smaller molecular weight proteins and peptidases; the latter can be divided into carboxypeptidases and aminopeptidases, which hydrolyze polypeptide chains one by one from the free carboxyl end or free amino end of the polypeptide to generate amino acids.
[0003] The existing chromatographic extraction method of protease is mainly through gel filtration. The principle of gel filtration chromatography is to utilize the degree of adsorption of different molecules by gel particles to extract protease. In the existing protease chromatographic extraction, the staff mostly holds a glass rod and extends it into the chromatography column for diversion. As a result, it is difficult to maintain the stability of the glass rod during the process of adding the solution, resulting in the solution not being able to be added to the chromatography column stably and uniformly. As a result, air is generated when the solution enters the chromatography column, interfering with the interaction between the protease and the chromatography column filler, thereby reducing the chromatography effect. Summary of the invention
[0004] The purpose of the present invention is to provide a protease chromatography extraction device and extraction method, aiming to solve the technical problem in the prior art that the solution cannot be stably added, resulting in the generation of air when the solution enters the chromatography column.
[0005] The present invention is achieved as follows: a protease chromatography extraction device comprises a base, a vertical plate is fixedly mounted on the base, a clamping assembly is arranged on the vertical plate, a chromatography column clamped by the clamping assembly is arranged on the vertical plate, a second horizontal plate is slidably mounted on the vertical plate, a second adjusting threaded rod is rotatably mounted on the vertical plate to drive the second horizontal plate to move, a rotating plate is rotatably mounted on the second horizontal plate, a rotating power part for driving the rotating plate to rotate is fixedly mounted on the second horizontal plate, two groups of parallel brackets are fixedly mounted on the rotating plate, a mobile frame is slidably mounted on the bracket, the mobile frame is a frame-type structure and a mounting block is rotatably mounted inside the mobile frame, a guide glass rod is slidably mounted on the mounting block, and an adjusting assembly for adjusting the angle of the mounting block is arranged on the mobile frame.
[0006] Further technical solution: the axis of the chromatography column and the axis of rotation of the rotating plate are located on the same vertical line.
[0007] Further technical solution: The clamping assembly includes a first horizontal plate, which is slidably mounted on the vertical plate and fixed by fixing screws, a mounting frame is fixedly mounted on the end of the first horizontal plate away from the vertical plate, two groups of symmetrically distributed movable plates are slidably mounted on the mounting frame, a clamping plate is fixedly mounted on the end of the movable plate away from the mounting frame, and a first adjusting threaded rod is rotatably mounted on the mounting frame to drive the two groups of movable plates to move in opposite directions at the same time.
[0008] Further technical solution: the cross section of the clamping plate is a V-shaped structure, and the openings of the two groups of clamping plates are opposite to each other, and the chromatography column is in conflict with the two groups of clamping plates.
[0009] Further technical solution: the adjustment component includes a sliding rod, which is slidably installed on the mobile frame and points to the mounting block. A limiting rod is fixedly installed on one end of the sliding rod close to the mounting block. An elastic member distributed around the sliding rod is fixedly installed on the mobile frame. One end of the elastic member away from the mobile frame is fixedly connected to one end of the sliding rod close to the limiting rod. A third adjusting threaded rod is threadedly installed on one end of the mobile frame away from the sliding rod. The third adjusting threaded rod points to the mounting block and conflicts with the mounting block.
[0010] Further technical solution: a telescopic rod is fixedly installed on the rotating plate, the telescopic rod points to the base and a lifting plate is fixedly installed on one end of the telescopic rod close to the base, a barrel is arranged on the lifting plate, a drop pipe is connected to the barrel, the drop pipe is in sliding contact with the lifting plate and the drop pipe passes through the lifting plate, a cover plate is threadedly installed on the barrel, and air holes are opened on the side wall of the cover plate.
[0011] Further technical solution: the axis of the blanking pipe and the rotation axis of the rotating plate are located on the same vertical line, the blanking pipe and the guide glass rod are distributed in the same plane, and a manual valve is provided on the blanking pipe.
[0012] Further technical solution: a shielding cylinder is fixedly installed on the lifting plate, the axis of the shielding cylinder coincides with the axis of the blanking tube, the blanking tube extends into the shielding cylinder, and a through hole is opened on the top side wall of the shielding cylinder for the guide glass rod to pass through, an elastic membrane is fixedly installed in the through hole, an elastic coil is fixedly connected to the middle of the elastic membrane, the elastic coil is sleeved on the guide glass rod, and the flat area of the elastic membrane is larger than the area of the through hole.
[0013] Further technical solution: A coaxially arranged shielding ring is slidably mounted on the shielding tube, an internal thread is provided on the top of the shielding ring, and external threads that cooperate with the internal thread are provided on the upper and lower ends of the shielding tube.
[0014] The present invention also provides a protease chromatography extraction method, which is applied to the protease chromatography extraction device described above, and comprises the following steps: Step S1: clamping the chromatography column by a clamping assembly and adjusting the height of the chromatography column; Step S2: adjusting the angle of the guide glass rod by adjusting the assembly, then adjusting the length of the guide glass rod extending downward, and then rotating the second adjusting threaded rod to drive the second horizontal plate to move downward so that the lower end of the guide glass rod enters the chromatography column, so that the lower end of the guide glass rod contacts the inner wall of the chromatography column; Step S3: A staff member holds a solution containing protease and adds the solution into a chromatography column through a guide glass rod for chromatography; Step S4: After the set time, the staff adds the buffer solution into the chromatography column through the guide glass rod to complete the chromatography.
[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. Adjust the angle of the guide glass rod by adjusting the assembly, then adjust the length of the guide glass rod extending downward, and then make the lower end of the guide glass rod enter the chromatography column, so that the lower end of the guide glass rod contacts the inner wall of the chromatography column, and add the solution into the chromatography column through the guide glass rod. The solution moves into the chromatography column along the guide glass rod and then flows downward along the inner wall of the chromatography column. Since the guide glass rod is in a fixed state, the solution can stably flow downward along the inner wall of the chromatography column, reducing the generation of bubbles when the solution is added, avoiding interference with the interaction between the protease and the chromatography column filler, and can make the protease solution evenly distributed in the chromatography column, thereby improving the chromatography effect.
[0016] 2. After the angle adjustment of the guide glass rod is completed, adjust the height of the lifting plate so that the lower end of the drop tube contacts the guide glass rod. During chromatography, open the manual valve on the drop tube, and the solution can drop directly onto the guide glass rod from the drop tube, so that the solution can stably transfer to the guide glass rod and flow downward along the guide glass rod, avoiding the splashing of the solution and improving the stability of the solution during dropping. At the same time, since the contact position between the drop tube and the guide glass rod is closer to the chromatography column, the distance and time of the solution flowing on the guide glass rod are reduced, the probability of impurities in the air entering the solution is reduced, and the chromatography effect is further improved.
[0017] 3. During chromatography, the rotating plate is driven to rotate slowly by the rotating power part. At this time, the guide glass rod and the barrel will rotate synchronously. Since the axis of the drop tube and the rotating axis of the rotating plate are located on the same vertical line, the solution will keep falling in a straight line to avoid affecting the flow of the solution to the guide glass rod. When the guide glass rod rotates, its contact position with the chromatography column will change into a circular shape with the inner wall of the chromatography column, so that the solution flowing down from the guide glass rod can enter and be distributed in the chromatography column more evenly, further improving the chromatography effect.
[0018] 4. During chromatography, the internal thread on the shielding ring is connected to the external thread below the shielding tube, and the lower surface of the shielding ring is lower than the upper surface of the chromatography column, so that the area where the solution flows on the guide glass rod is shielded by the shielding tube and the shielding ring, and the probability of impurities entering the solution in the process of the solution entering the chromatography column from the barrel is further reduced, thereby further improving the purity of the solution and the chromatography effect. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention.
[0020] Figure 2 It is a schematic diagram of the structure of the rotating plate in the present invention.
[0021] Figure 3 It is a schematic diagram of the cross-sectional structure of the shielding tube in the present invention.
[0022] Figure 4 It is a schematic diagram of the structure of the clamping assembly in the present invention.
[0023] Figure 5 for Figure 3 A magnified schematic diagram of the A1 region in the middle.
[0024] Figure 6 for Figure 3 A magnified schematic diagram of the A2 region in the middle.
[0025] In the attached drawings: 1. base; 2. vertical plate; 3. first horizontal plate; 4. mounting frame; 5. movable plate; 6. clamping plate; 7. first adjusting threaded rod; 8. chromatography column; 9. second horizontal plate; 10. second adjusting threaded rod; 11. rotating power part; 12. rotating plate; 13. bracket; 14. movable frame; 15. mounting block; 16. guide glass rod; 17. sliding rod; 18. limiting rod; 19. elastic part; 20. third adjusting threaded rod; 21. telescopic rod; 22. lifting plate; 23. barrel; 24. drop pipe; 25. cover plate; 26. shielding barrel; 27. shielding ring; 28. elastic membrane; 29. elastic coil; 30. clamping assembly; 31. adjusting assembly. DETAILED DESCRIPTION
[0026] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.
[0027] The specific implementation of the present invention is described in detail below in conjunction with specific embodiments.
[0028] like Figure 1-Figure 6As shown, a protease chromatography extraction device provided by the present invention comprises a base 1, a vertical plate 2 is fixedly mounted on the base 1, a clamping assembly 30 is arranged on the vertical plate 2, a chromatography column 8 clamped by the clamping assembly 30 is arranged on the vertical plate 2, a second horizontal plate 9 is slidably mounted on the vertical plate 2, a second adjusting threaded rod 10 is rotatably mounted on the vertical plate 2 to drive the second horizontal plate 9 to move, a rotating plate 12 is rotatably mounted on the second horizontal plate 9, a rotating power member 11 is fixedly mounted on the second horizontal plate 9 to drive the rotating plate 12 to rotate, the axis of the chromatography column 8 and the axis of rotation of the rotating plate 12 are located on the same vertical line, two groups of parallel brackets 13 are fixedly mounted on the rotating plate 12, a moving frame 14 is slidably mounted on the bracket 13, the moving frame 14 is a frame-type structure and a mounting block 15 is rotatably mounted in the moving frame 14, a guide glass rod 16 is slidably mounted on the mounting block 15, and an adjusting assembly 31 for adjusting the angle of the mounting block 15 is arranged on the moving frame 14.
[0029] In actual application of this embodiment, the chromatography column 8 is clamped by the clamping assembly 30, and then the angle of the guide glass rod 16 is adjusted by the adjusting assembly 31. The fixing screws on the mounting block 15 that fix the guide glass rod 16 can adjust the downward extension length of the guide glass rod 16. The second adjusting threaded rod 10 is rotated to drive the second horizontal plate 9 to move downward so that the lower end of the guide glass rod 16 enters the chromatography column 8. After the adjustment is completed, the lower end of the guide glass rod 16 contacts the inner wall of the chromatography column 8. The rotating plate 12 is driven to rotate by the rotating power member 11 to adjust the position of the guide glass rod 16, so that the subsequent solution can be drained through the guide glass rod 16. After the adjustment is completed, the staff holds the solution containing protease and adds the solution to the chromatography column 8 through the guide glass rod 16. The solution moves into the chromatography column 8 along the guide glass rod 16 and then flows downward along the inner wall of the chromatography column 8. Since the guide glass rod 16 is in a fixed state, the solution can stably flow downward along the inner wall of the chromatography column 8, reducing the generation of bubbles when the solution is added, avoiding interference with the interaction between the protease and the chromatography column 8 filler, and enabling the protease solution to be evenly distributed in the chromatography column 8, thereby improving the chromatography effect. Afterwards, the buffer solution is added to the chromatography column 8 through the guide glass rod 16 to complete the chromatography.
[0030] like Figure 1 , Figure 4 As shown, a protease chromatography extraction device provided by the present invention, the clamping assembly 30 includes a first horizontal plate 3, the first horizontal plate 3 is slidably mounted on the vertical plate 2 and fixed by fixing screws, a mounting frame 4 is fixedly mounted on one end of the first horizontal plate 3 away from the vertical plate 2, two groups of symmetrically distributed moving plates 5 are slidably mounted on the mounting frame 4, a clamping plate 6 is fixedly mounted on one end of the moving plate 5 away from the mounting frame 4, and a first adjusting threaded rod 7 is rotatably mounted on the mounting frame 4 to drive the two groups of moving plates 5 to move in opposite directions at the same time.
[0031] Specifically, the cross section of the clamping plates 6 is a V-shaped structure, and the openings of the two groups of clamping plates 6 are opposite to each other, and the chromatography column 8 is in conflict with the two groups of clamping plates 6 .
[0032] In actual application of this embodiment, the chromatography column 8 is placed between the two groups of clamping plates 6, and the first adjusting threaded rod 7 is rotated to drive the two groups of movable plates 5 to move inward at the same time. When the movable plate 5 drives the clamping plate 6 to move, the clamping plate 6 contacts the surface of the chromatography column 8, and then the chromatography column 8 is clamped and fixed by the clamping plate 6. Due to the V-shaped structure of the clamping plate 6, the positioning of the chromatography column 8 is achieved, ensuring that the axis of the chromatography column 8 and the rotation axis of the rotating plate 12 are located on the same vertical line. The height of the chromatography column 8 can be adjusted by adjusting the position of the first horizontal plate 3, and then the first horizontal plate 3 can be fixed by fixing screws.
[0033] like Figure 1-Figure 3 , Figure 5 As shown, a protease chromatography extraction device provided by the present invention, the adjustment component 31 includes a slide rod 17, the slide rod 17 is slidably installed on the movable frame 14 and the slide rod 17 points to the mounting block 15, a limit rod 18 is fixedly installed on one end of the slide rod 17 close to the mounting block 15, an elastic member 19 distributed around the slide rod 17 is fixedly installed on the movable frame 14, an end of the elastic member 19 away from the movable frame 14 is fixedly connected to an end of the slide rod 17 close to the limit rod 18, and a third adjustment threaded rod 20 is threadedly installed on one end of the movable frame 14 away from the slide rod 17, and the third adjustment threaded rod 20 points to the mounting block 15 and conflicts with the mounting block 15.
[0034] In actual application of this embodiment, the third adjusting threaded rod 20 is rotated. When the third adjusting threaded rod 20 moves to the side close to the limiting rod 18, it will push the mounting block 15 to rotate and further compress the elastic member 19. When the third adjusting threaded rod 20 moves to the side away from the limiting rod 18, since the elastic member 19 is in a compressed state, the mounting block 15 is rotated in the opposite direction under the action of the elastic member 19, so that the mounting block 15 is always in contact with the third adjusting threaded rod 20. The mounting block 15 can be rotated to adjust the inclination angle of the guide glass rod 16. The inclination angle of the guide glass rod 16 can be adjusted according to the flow rate of the solution and the diameter of the chromatography column 8, so that the solution can enter the chromatography column 8 evenly and smoothly, thereby improving the flexibility of device adjustment, ensuring the uniformity of solution flow, and improving the chromatography effect.
[0035] In an embodiment of the present invention, the elastic member 19 is a spring, and of course it can also be other elastic members such as an elastic ball, and the spring is compressed to apply a thrust to the mounting block 15 .
[0036] like Figure 1-Figure 3 , Figure 6As shown, a protease chromatography extraction device provided by the present invention is provided, a telescopic rod 21 is fixedly installed on the rotating plate 12, the telescopic rod 21 points to the base 1 and a lifting plate 22 is fixedly installed on the end of the telescopic rod 21 close to the base 1, a barrel 23 is arranged on the lifting plate 22, the barrel 23 is connected with a drop pipe 24, the drop pipe 24 is in sliding contact with the lifting plate 22 and the drop pipe 24 passes through the lifting plate 22, a cover plate 25 is threadedly installed on the barrel 23, and air holes are opened on the side wall of the cover plate 25.
[0037] Specifically, the axis of the blanking pipe 24 and the rotation axis of the rotating plate 12 are located on the same vertical line, the blanking pipe 24 and the guide glass rod 16 are distributed in the same plane, and a manual valve is provided on the blanking pipe 24 .
[0038] In actual application of this embodiment, after the angle adjustment of the guide glass rod 16 is completed, the height of the lifting plate 22 is adjusted so that the lower end of the drop tube 24 contacts the guide glass rod 16, and then the solution can be introduced into the barrel 23 and covered with the cover plate 25. During chromatography, the lower end of the guide glass rod 16 enters the chromatography column 8 and contacts the inner wall of the chromatography column 8. The manual valve on the drop tube 24 is opened, and the solution can directly fall from the drop tube 24 onto the guide glass rod 16, so that the solution can be stably transferred to the guide glass rod 16 and flow downward along the guide glass rod 16, avoiding the solution from splashing and improving the stability of the solution when dropping. At the same time, because the contact position of the drop tube 24 and the guide glass rod 16 is closer to the chromatography column 8, the distance and time of the solution flowing in the guide glass rod 16 are reduced, the probability of impurities in the air entering the solution is reduced, and the chromatography effect is further improved. During chromatography, the rotating plate 12 is driven to rotate slowly by the rotating power part 11. At this time, the guide glass rod 16 and the barrel 23 will rotate synchronously. Since the axis of the drop tube 24 and the rotation axis of the rotating plate 12 are located on the same vertical line, the solution will keep falling in a straight line to avoid affecting the flow of the solution to the guide glass rod 16. When the guide glass rod 16 rotates, its contact position with the chromatography column 8 will change into a circular shape with the inner wall of the chromatography column 8, so that the solution flowing down from the guide glass rod 16 can enter and be distributed in the chromatography column 8 more evenly, further improving the chromatography effect. The same operation is performed when the buffer solution is added later.
[0039] like Figure 1-Figure 3 As shown, a protease chromatography extraction device provided by the present invention is shown. In actual application of this embodiment, a shielding tube 26 is fixedly installed on the lifting plate 22, and the axis of the shielding tube 26 coincides with the axis of the blanking tube 24. The blanking tube 24 extends into the shielding tube 26. A through hole is opened on the top side wall of the shielding tube 26 for the guide glass rod 16 to pass through. An elastic membrane 28 is fixedly installed in the through hole, and an elastic coil 29 is fixedly connected to the middle part of the elastic membrane 28. The elastic coil 29 is sleeved on the guide glass rod 16, and the flat area of the elastic membrane 28 is larger than the area of the through hole.
[0040] Specifically, a coaxially arranged shielding ring 27 is slidably mounted on the shielding tube 26 , an internal thread is provided on the top of the shielding ring 27 , and external threads that cooperate with the internal thread are provided on the upper and lower ends of the shielding tube 26 .
[0041] In actual application of this embodiment, the guide glass rod 16 passes through the elastic coil 29, and the elastic coil 29 can fit tightly on the guide glass rod 16 to complete the sealing. Since the flat area of the elastic membrane 28 is larger than the area of the through hole, the adjustment of the inclination angle of the guide glass rod 16 is avoided. When adjusting the angle of the guide glass rod 16, the internal thread on the shielding ring 27 is connected to the external thread above the shielding tube 26, thereby avoiding blocking the squatting of the guide glass rod 16 and facilitating the adjustment of the guide glass rod 16. During subsequent chromatography, the internal thread on the shielding ring 27 is connected to the external thread below the shielding tube 26, and the lower surface of the shielding ring 27 is lower than the upper surface of the chromatography column 8, so that the area where the solution flows on the guide glass rod 16 is shielded by the shielding tube 26 and the shielding ring 27, and the probability of impurities entering the solution in the process of the solution entering the chromatography column 8 from the barrel 23 is further reduced, thereby further improving the purity of the solution and the chromatography effect.
[0042] like Figure 1-Figure 6 As shown, the present invention provides a protease chromatography extraction method, which is applied to the protease chromatography extraction device described above, and comprises the following steps: Step S1: clamping the chromatography column 8 by the clamping assembly 30 and adjusting the height of the chromatography column 8; Step S2: adjusting the angle of the guide glass rod 16 by adjusting the assembly 31, then adjusting the length of the guide glass rod 16 extending downward, and then rotating the second adjusting threaded rod 10 to drive the second horizontal plate 9 to move downward so that the lower end of the guide glass rod 16 enters the chromatography column 8, so that the lower end of the guide glass rod 16 contacts the inner wall of the chromatography column 8; Step S3: The staff holds the solution containing the protease and adds the solution into the chromatography column 8 through the guide glass rod 16 for chromatography; Step S4: After the set time, the staff adds the buffer solution into the chromatography column 8 through the guide glass rod 16 to complete the chromatography.
[0043] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
[0044] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.
Claims
1. A protease chromatography extraction device, comprising a base (1), on which a vertical plate (2) is fixedly mounted, characterized in that: The vertical plate (2) is provided with a clamping assembly (30), the vertical plate (2) is provided with a chromatography column (8) clamped by the clamping assembly (30), the vertical plate (2) is slidably mounted with a second horizontal plate (9), the vertical plate (2) is rotatably mounted with a second adjusting threaded rod (10) for driving the second horizontal plate (9) to move, the second horizontal plate (9) is rotatably mounted with a rotating plate (12), the second horizontal plate (9) is fixedly mounted with a rotating power member (11) for driving the rotating plate (12) to rotate, the rotating plate (12) is fixedly mounted with two sets of parallel brackets (13), a moving frame (14) is slidably mounted on the bracket (13), the moving frame (14) is a frame-type structure, a mounting block (15) is rotatably mounted inside the moving frame (14), a guide glass rod (16) is slidably mounted on the mounting block (15), and an adjusting assembly (31) for adjusting the angle of the mounting block (15) is provided on the moving frame (14).
2. A protease chromatography extraction device according to claim 1, characterized in that: The axis of the chromatography column (8) and the axis of rotation of the rotating plate (12) are located on the same vertical line.
3. A protease chromatography extraction device according to claim 1, characterized in that: The clamping assembly (30) comprises a first transverse plate (3), the first transverse plate (3) being slidably mounted on the vertical plate (2) and fixed by fixing screws, a mounting frame (4) being fixedly mounted on one end of the first transverse plate (3) away from the vertical plate (2), two groups of symmetrically distributed moving plates (5) being slidably mounted on the mounting frame (4), a clamping plate (6) being fixedly mounted on one end of the moving plate (5) away from the mounting frame (4), and a first adjusting threaded rod (7) being rotatably mounted on the mounting frame (4) for driving the two groups of moving plates (5) to move in opposite directions simultaneously.
4. A protease chromatography extraction device according to claim 3, characterized in that: The cross section of the clamping plates (6) is a V-shaped structure, and the openings of the two groups of clamping plates (6) are opposite to each other, and the chromatography column (8) is in contact with the two groups of clamping plates (6).
5. A protease chromatography extraction device according to claim 1, characterized in that: The adjustment assembly (31) comprises a slide bar (17), the slide bar (17) being slidably mounted on the movable frame (14) and the slide bar (17) pointing to the mounting block (15), a limit rod (18) being fixedly mounted on one end of the slide bar (17) close to the mounting block (15), an elastic member (19) distributed around the slide bar (17) being fixedly mounted on the movable frame (14), an end of the elastic member (19) away from the movable frame (14) being fixedly connected to an end of the slide bar (17) close to the limit rod (18), and a third adjustment threaded rod (20) being threadedly mounted on one end of the movable frame (14) away from the slide bar (17), the third adjustment threaded rod (20) pointing to the mounting block (15) and abutting against the mounting block (15).
6. A protease chromatography extraction device according to claim 1, characterized in that: A telescopic rod (21) is fixedly mounted on the rotating plate (12), the telescopic rod (21) points toward the base (1), and a lifting plate (22) is fixedly mounted on one end of the telescopic rod (21) close to the base (1), a material barrel (23) is arranged on the lifting plate (22), a material drop pipe (24) is connected to the material barrel (23), the material drop pipe (24) is in sliding contact with the lifting plate (22), and the material drop pipe (24) passes through the lifting plate (22), a cover plate (25) is threadedly mounted on the material barrel (23), and air holes are opened on the side wall of the cover plate (25).
7. A protease chromatography extraction device according to claim 6, characterized in that: The axis of the blanking pipe (24) and the rotation axis of the rotating plate (12) are located on the same vertical line, the blanking pipe (24) and the guide glass rod (16) are distributed in the same plane, and a manual valve is provided on the blanking pipe (24).
8. A protease chromatography extraction device according to claim 7, characterized in that: A shielding tube (26) is fixedly mounted on the lifting plate (22), the axis of the shielding tube (26) coincides with the axis of the blanking tube (24), the blanking tube (24) extends into the shielding tube (26), a through hole is provided on the top side wall of the shielding tube (26) for the guide glass rod (16) to pass through, an elastic membrane (28) is fixedly mounted in the through hole, an elastic coil (29) is fixedly connected to the middle of the elastic membrane (28), the elastic coil (29) is sleeved on the guide glass rod (16), and the flattened area of the elastic membrane (28) is larger than the area of the through hole.
9. A protease chromatography extraction device according to claim 8, characterized in that: A coaxially arranged shielding ring (27) is slidably mounted on the shielding cylinder (26); an internal thread is provided on the top of the shielding ring (27); and external threads that cooperate with the internal thread are provided on the upper and lower ends of the shielding cylinder (26).
10. A protease chromatography extraction method, characterized in that: A protease chromatography extraction device as described in any one of claims 1 to 9, comprising the following steps: Step S1: clamping the chromatography column (8) by means of the clamping assembly (30) and adjusting the height of the chromatography column (8); Step S2: adjusting the angle of the guide glass rod (16) by means of the adjusting assembly (31), then adjusting the length of the guide glass rod (16) extending downward, and then rotating the second adjusting threaded rod (10) to drive the second horizontal plate (9) to move downward so that the lower end of the guide glass rod (16) enters the chromatography column (8), so that the lower end of the guide glass rod (16) contacts the inner wall of the chromatography column (8); Step S3: A worker holds a solution containing protease and adds the solution to the chromatography column (8) through a guide glass rod (16) for chromatography; Step S4: After the set time, the staff adds the buffer solution into the chromatography column (8) through the guide glass rod (16) to complete the chromatography.