Sampling and collecting device for intestinal flora gene detection

Abstract

The invention discloses a sampling and collecting device for intestinal flora gene detection, the sampling and collecting device comprises a collecting storage bottle and a collecting assembly, the collecting assembly comprises a suction sealing cover, a collecting pipe fitting, a sealing cover and a sealing sleeve, and the suction sealing cover comprises an elastic bag, a sealing joint, a sealing plug and a connecting rod; the sealing cover comprises a cover body and a protruding pipe part, the upper end of the protruding pipe part is connected with the sealing connector, a sealing sleeve clamping groove is formed in the lower portion of the cover body, and a liquid outlet hole and a guide groove are formed in the wall face of the sealing sleeve clamping groove. The sealing sleeve is fixedly connected in the sealing sleeve clamping groove; the collecting pipe fitting comprises a straight pipe with the upper portion inserted into the sealing cover, a hook groove, a side hole, a baffle ring and a collecting spoon are sequentially arranged on the straight pipe from top to bottom, and when the upper face of the baffle ring makes contact with the lower face of the clamping groove of the sealing sleeve, the side hole can coincide with the liquid outlet hole; a bottle body of the collecting and storing bottle is provided with an annular clamping groove and a corrugated pipe section, the annular clamping groove is connected with a C-shaped clamping ring, and a bottle body opening is connected with the cover body. The sample collection process is simpler, pollution is not prone to occurring, consumption of consumables in the follow-up detection link is small, and operation is more convenient and efficient.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a sampling and collecting device for intestinal flora gene detection. Background Art

[0002] The human intestinal flora is closely related to a variety of diseases. When the dynamic balance between the intestinal flora, the human body and the external environment is broken, intestinal flora imbalance will occur, which will in turn cause disease or aggravate the original condition.

[0003] The detection of intestinal flora is a common test item in modern gastroenterology. Its principle is mainly based on high-throughput sequencing technology and bioinformatics analysis of modern biotechnology. By analyzing the DNA sequence of the microbial genome in the sample, the composition and diversity of the individual's intestinal flora can be revealed. This detection method is of great significance for understanding human health, disease mechanisms and personalized medicine.

[0004] To obtain bacterial samples from the intestines of patients or experimental subjects, the common ways to obtain samples are fecal sampling and mucosal sampling. Among them, fecal sampling is a more common sampling method. The most common sampling process requires the experimental subject to use a sterilized sampling cotton swab to directly obtain fecal samples from toilet paper or feces in the toilet during defecation, and then put the fecal sample into the reagent solution tube and shake it well before sending it for testing. Although this method is simple and easy to use, it still has the following problems: 1. After the tester receives the sample to be tested, he needs to open the sample storage bottle and use a handheld pipette or other tools to extend into the storage bottle for sampling. Not only does it consume the pipette tip, but it also needs to operate the pipette, which increases the operation steps and pollution links; 2. The sampling cotton swab needs to be inserted into the storage bottle and stirred and eluted before being discarded. Due to the personal quality of the collector and other issues, there is a potential risk of contamination and safety. In addition, the operation effects of different people in the process of opening the cover, stirring and eluting the sample vary greatly, and the elution may be unqualified, and it is also easy to cause problems such as splashing of dirt. Summary of the invention

[0005] The purpose of the present invention includes providing a sampling and collection device for intestinal flora gene detection that does not require the collector to elute the sample specifically or the elution operation is easier, the sample elution process is not easy to be contaminated, the subsequent detection operation link consumes less consumables, and the operation is more convenient and efficient.

[0006] In order to achieve the above object, the present invention has the following technical solutions:

[0007] The present invention provides a sampling and collecting device for intestinal flora gene detection, comprising a collection storage bottle and a collection component, wherein the collection component comprises a suction sealing cover, a collection pipe, a sealing cover and a sealing sleeve.

[0008] The suction seal cover comprises an elastic bag, a sealing joint, a sealing plug and at least two connecting rods, the lower side of the connecting rods is connected with a barb, the connecting rods are connected between the sealing plug and the inner upper end of the sealing joint through their two ends, the upper end of the sealing joint is sealed and connected to the lower end opening of the elastic bag, the lower part of the sealing joint is provided with a detachable connection structure such as a thread, and the sealing joint and the elastic bag are connected through the through hole between the connecting rods;

[0009] The sealing cover comprises a cover body and a convex tube portion vertically connected to the cover body, the upper end of the convex tube portion is provided with a joint such as a thread for detachably connecting a sealing joint, the lower side of the cover body is provided with a sealing sleeve groove coinciding with the axis of the convex tube portion, the upper wall surface of the sealing sleeve groove is provided with a liquid outlet hole, the outer wall surface is provided with a guide groove, the outer wall surface of the sealing sleeve groove is provided with an installation indicator line near the guide groove, and the cover body is detachably connected to the mouth of the collection storage bottle;

[0010] The sealing sleeve is connected in the sealing sleeve slot, and the liquid outlet hole on the upper wall of the sealing sleeve coincides with the liquid outlet hole on the sealing sleeve slot;

[0011] The collection tube comprises a straight tube, the upper portion of which is sealed and inserted into a sealing cover after passing through a sealing sleeve. The upper end of the straight tube is provided with a hook groove for connecting with the barb hook, the lower end is provided with a collection spoon for collecting samples, the middle portion is provided with a retaining ring, and the wall surface of the straight tube is provided with a side hole, the side hole is located at the upper portion of the retaining ring and under the sealing sleeve, the upper edge of the retaining ring is vertically connected with an anti-rotation limit rod for slidingly inserting with the guide groove, and when the upper portion of the retaining ring contacts the lower portion of the sealing sleeve slot, the side hole can coincide with the liquid outlet.

[0012] Preferably, the inner wall surface of the sealing sleeve is radially provided with a plurality of convex rings, which are similar to the inner convex rings of the sealing ring for the solar vacuum tube.

[0013] Preferably, the inner diameter of the convex ring is smaller than the outer diameter of the straight tube.

[0014] Preferably, the elastic bag is a corrugated blind tube integrally formed with the upper end of the sealing joint.

[0015] Preferably, a spring is connected between the corrugated blind pipe and the sealing joint.

[0016] Preferably, a sampling marking line is radially provided on the outer wall surface of the straight tube.

[0017] Preferably, the wall surface of the convex tube portion is provided with two opposite long holes, through which the straight tube can be contacted.

[0018] Preferably, the inner hole of the convex tube portion is a tapered hole whose diameter gradually increases towards the cover body.

[0019] Preferably, the diameter of the tapered hole is larger than the outer diameter of the straight tube.

[0020] Preferably, the bottom surface of the sealing plug is in the shape of a spherical surface or a conical surface.

[0021] Preferably, a pointed protrusion is connected to the lower end of the collecting spoon.

[0022] Preferably, the body of the collection storage bottle comprises a bellows section which is integrally formed with the body and is easily compressible.

[0023] Preferably, the outer wall surface of the body of the collection storage bottle includes at least two annular grooves, the annular grooves are connected with C-shaped clamping rings, and the bellows section is located between the annular grooves and below the C-shaped clamping ring.

[0024] Preferably, the collection storage bottle contains a preservation solution.

[0025] Preferably, the body of the collection storage bottle is made of polypropylene plastic or high-density polyethylene plastic.

[0026] Preferably, the collection tube is made of polymethyl methacrylate plastic.

[0027] Preferably, the C-shaped clamping ring is made of polymethyl methacrylate plastic or ABS plastic.

[0028] Preferably, the collection component and the collection storage bottle included in the present invention are independently aseptically packaged in the same packaging box, and the collection component is aseptically packaged in a ready-to-collect state and placed in a packaging slot that matches its shape in the ready-to-collect state.

[0029] Due to the adoption of the above technical solution, the advantages of the present invention are:

[0030] 1. After receiving the present invention containing the sample to be tested, the testing personnel can remove the suction cover from the upper end of the convex tube part, and directly add the sample to be tested by pointing the hook groove end of the straight tube toward the mouth of the centrifugal tube such as the EP tube, without using sample transfer tools such as a pipette, saving pipette tips, fewer operation links, and more convenient and efficient operation.

[0031] 2. The present invention can perform shaking elution and mixing operations after sealing, or no special elution and mixing operations are required, and the elution and mixing of the preservation solution and the sample can be achieved during transportation. The elution and mixing operations without special elution or shaking are easier than opening the lid and stirring for elution, and the elution effect is better. It is also not easy to cause splashing of dirt during stirring as in the prior art, and pipetting contamination in the detection and sampling process.

[0032] 3. The present invention can also wash away the feces adhering to the collection spoon with the sucked liquid by squeezing the upper end of the corrugated blind tube before the cover body is sealed with the collection storage bottle, thereby ensuring that the sample can be splash-proof and mixed with the preservation solution more quickly.

[0033] 4. The present invention has a pointed protrusion at the lower end of the collection spoon, which is conducive to breaking up relatively dry feces for sampling. In addition, the present invention also has sampling methods such as scooping, sucking, and scraping, which can better adapt to the sample collection of feces with various characteristics.

[0034] 5. The collection storage bottle of the present invention is provided with a preservation solution, which can maintain the vitality of the microorganisms in the sample and ensure the accuracy of the experimental data. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0036] Figure 1 A schematic front view of a sampling and collecting device for intestinal flora gene detection provided by an embodiment of the present invention;

[0037] Figure 2 for Figure 1 A schematic diagram of the main view of the collection component after removing the suction cover;

[0038] Figure 3 for Figure 2 Schematic diagram of top view;

[0039] Figure 4 for Figure 1 A bottom view of the suction cover;

[0040] Figure 5 It is a schematic axial partial cross-sectional view of the collection assembly when it is pre-connected to the collection storage bottle;

[0041] Figure 6 for Figure 1 A schematic diagram of an axial partial cross-section;

[0042] Figure 7 for Figure 6 A magnified schematic diagram of point A;

[0043] Figure 8 For the general Figure 6 A schematic diagram of an axial partial cross-section after the suction seal cover is removed.

[0044] In the figure: 1, thread; 2, liquid outlet; 3, through hole; 10, collection component; 11, suction sealing cover; 111, corrugated blind pipe; 112, sealing joint; 113, connecting rod; 1131, barb; 114, sealing plug; 12, collection pipe fitting; 121, straight pipe; 122, side hole; 123, retaining ring; 1231, anti-rotation limit rod; 124, collection spoon; 125, pointed protrusion; 126, hook groove; 127, sampling mark line; 13, sealing cover; 131, convex pipe part; 1311, long hole; 132, cover body; 1321, sealing sleeve slot; 1322, guide groove; 1323, installation indicator line; 14, sealing sleeve; 20, collection storage bottle; 21, annular slot; 22, corrugated pipe section; 30, C-shaped clamping ring; 31, inner convex clamping strip. DETAILED DESCRIPTION

[0045] In the description of the present invention, it should be noted that if the terms "upper", "lower", "inside", "outside", "left", "right", "front", "back" and the like appear to indicate directions or positional relationships, they are based on the directions or positional relationships shown in the accompanying drawings, or are the directions or positional relationships in which the inventive product is usually placed when in use. These are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.

[0046] It should be noted that, in the absence of conflict, the features in the embodiments of the present invention may be combined with each other.

[0047] The specific implementation method is now described as follows:

[0048] An embodiment of a sampling and collecting device for intestinal flora gene detection provided by the present invention is shown in Figure 1 , Figure 5 , including a collection storage bottle 20 and a collection component 10,

[0049] The collection assembly 10 includes a suction sealing cover 11, a collection pipe 12, a sealing cover 13 and a sealing sleeve 14, wherein the suction sealing cover 11 includes a sealing joint 112 with a thread 1 on the inner surface, a corrugated blind tube 111 integrally formed with the upper end of the sealing joint 112, a spring connected between the corrugated blind tube 111 and the sealing joint 112, a sealing plug 114 and three connecting rods 113, the lower side of the connecting rod 113 is connected with a barb 1131, the connecting rod 113 is connected between the sealing plug 114 and the inner upper end of the sealing joint 112 through its two ends, the lower side of the sealing plug 114 is spherical, the sealing joint 112 and the corrugated blind tube 111 are connected by a through hole 3 between the connecting rods 113, see Figure 1 , Figure 4 , Figure 5 ;

[0050] The sealing cover 13 comprises a cover body 132 and a convex tube portion 131 vertically connected to the cover body 132, the upper end of the convex tube portion 131 is provided with a thread 1 for threading the sealing joint 112, the wall surface of the convex tube portion 131 is provided with two opposite long holes 1311, through which the straight tube 121 can be contacted, the inner hole of the convex tube portion 131 is a tapered hole with a gradually increasing aperture close to the cover body 132, and the aperture of the tapered hole is larger than the outer diameter of the straight tube 121; the lower side of the cover body 132 is provided with a sealing sleeve groove 1321 coinciding with the axis of the convex tube portion 131, the upper wall surface of the sealing sleeve groove 1321 is provided with a liquid outlet 2, and the outer wall surface is provided with a guide groove 1322, and the outer wall surface of the sealing sleeve groove 1321 is provided with an installation indicator line 1323 near the guide groove 1322, and the cover body 132 is threadedly connected to the mouth of the collection storage bottle 20, see Figure 1 , Figure 2 , Figure 3 , Figure 5 ;

[0051] The inner wall surface of the sealing sleeve 14 is radially provided with a plurality of convex rings, the inner diameter of the convex rings is smaller than the outer diameter of the straight tube 121, and the straight tube 121 can slide axially in the sealing sleeve 14 under external force. The sealing sleeve 14 is fixedly connected in the sealing sleeve groove 1321, and the liquid outlet hole 2 on the upper wall thereof coincides with the liquid outlet hole 2 on the sealing sleeve groove 1321, see Figure 2 , Figure 5 ;

[0052] The collection tube 12 comprises a straight tube 121, the upper end of which is provided with a hook groove 126 for hooking with the barb 1131, the lower end of which is provided with a collection spoon 124, the lower end of which is connected with a sharp protrusion 125, a retaining ring 123 is provided in the middle of the straight tube 121, and a side hole 122 and a sampling mark line 127 are provided on the wall of the straight tube 121, the side hole 122 is located at the upper part of the retaining ring 123 and is wrapped by the sealing sleeve 14, the upper edge of the retaining ring 123 is vertically connected with an anti-rotation limit rod 1231 for slidingly plugging with the guide groove 1322, the upper part of the straight tube 121 is sealed through the sealing sleeve 14 and then plugged into the sealing cover 13 (at this time, the upper end of the anti-rotation limit rod 1231 is located between the installation indicator lines 1323, that is, in the state of waiting for collection, for example Figure 5 The state of the acquisition component 10 shown in Figure 2 When the upper end of the retaining ring 123 contacts the lower side of the sealing sleeve groove 1321, the side hole 122 can overlap with the liquid outlet hole 2, see Figure 2 , Figure 3 , Figure 7 , Figure 8 .

[0053] The body of the collection storage bottle 20 includes a bellows section 22 which is easily compressed and is integrally formed with the body. The outer wall of the body of the collection storage bottle 20 is provided with two annular grooves 21, and the annular grooves 21 are clamped with C-shaped clamping rings 30. The bellows section 22 is located between the annular grooves 21 and below the C-shaped clamping ring 30. Figure 1 , Figure 6 , Figure 8 .

[0054] In this embodiment, the suction cover 11 and the body of the collecting storage bottle 20 are both made of polypropylene plastic; the collecting tube 12 is made of polymethyl methacrylate plastic; the C-shaped clamp 30 is made of polymethyl methacrylate plastic or ABS plastic; the collecting storage bottle 20 is filled with preservation liquid; the collecting assembly 10 and the collecting storage bottle 20 included in the present invention are independently aseptically packaged in the same packaging box, and the collecting assembly 12 is aseptically packaged in a state to be collected and then placed in a packaging groove that matches its appearance in the state to be collected.

[0055] Method for collecting stool samples of the present invention

[0056] A. Normal stool sample collection method:

[0057] 1. The sampler holds the collection assembly 10 at the long hole 1311 and uses the collection spoon 124 to collect about a flat spoonful of feces from the upper part of the feces or toilet paper;

[0058] 2. Insert the collection spoon 124 into the collection bottle 20, and then tighten the cover 132 on the mouth of the collection bottle 20 (if the sample is directly tested, shake the collection bottle 20 after tightening the cover 132 to dissolve the feces in the preservation solution), see Figure 6 At this time, the bottom of the collection storage bottle 20 pushes the lower end of the collection tube 12, so that the collection tube 12 moves up to the upper end of the straight tube 121 and is tightly sealed and connected to the bottom of the sealing plug 114.

[0059] Note: When the inspector finds that there is still feces adhered to the collection spoon 124 after receiving the sample, and the color of the preservation solution is too light to meet the requirements, the inspector can shake the collection storage bottle 20 to dissolve the feces adhered to the collection spoon 124 into the preservation solution.

[0060] B. Methods for collecting drier stool samples:

[0061] 1. The sampler holds the collection assembly 10 at the long hole 1311, inserts the collection spoon 124 into the preservation solution in the collection storage bottle 20, then presses down the corrugated blind tube 111 and releases the hand to suck part of the preservation solution into the straight tube 121, then moves the collection spoon 124 to the feces to squeeze the corrugated blind tube 111, moistens the feces with the preservation solution, and then uses the collection spoon 124 to take a flat spoon of feces;

[0062] 2. Same as step 2 of the above method for collecting normal stool samples.

[0063] C. Methods for collecting diarrhea samples:

[0064] 1. The sampler holds the collection assembly 10 at the long hole 1311, scoops up the feces with the collection spoon 124, and / or squeezes the corrugated blind tube 111 and uses the lower end of the straight tube 121 to suck the feces to the sampling mark line 127;

[0065] 2. Same as step 2 of the above method for collecting normal stool samples.

[0066] After receiving the present invention containing the sample to be tested, the test personnel use the obtained sample for testing, and turn the suction seal cover 11 end upward, screw the sealing joint 112 to remove the suction seal cover 11 from the upper end of the convex tube part 131 (in the process of screwing the sealing joint 112, the sealing joint 112 moves up on the upper end of the convex tube part 131, and at the same time, the hook groove 126 end of the straight tube 121 is pulled out of the convex tube part 131 by the barb 1131 to pull the hook groove 126. When the suction seal cover 11 is separated from the convex tube part 131, the side hole 122 moves up to coincide with the liquid outlet hole 2, see 2, Figure 8 ), and then place the hook groove 126 end of the straight tube 121 that exposes the upper end of the convex tube part 131 downward on the mouth of a centrifugal tube such as an EP tube, hold the body of the collection storage bottle 20 and use the heat of the hand to conduct to the bottle body, so that the pressure in the collection storage bottle 20 increases and the liquid drips from the hook groove 126 end to the EP tube and other centrifugal tubes for a set number of drops, and then the subsequent detection operation can be carried out.

[0067] If you want to speed up the dripping speed of the sample, use your fingers to remove the C-shaped snap ring 30 stuck on the annular groove 21 (it is better to block one end of the C-shaped snap ring 30 with your fingers, and then use another finger to remove it in the direction away from the annular groove 21. In this process, the two ends of the annular groove 21 are separated from each other, the mouth is expanded, and the inner convex card strip 31 inside is out of the annular groove 21). When the bottom of the collection storage bottle 20 is lightly pressed, the bellows section 22 on the collection storage bottle 20 is appropriately compressed and deformed, and the collection storage bottle 20 is reduced in volume, and the internal liquid drips out faster. It can also be set that when the bellows section 22 on the collection storage bottle 20 is fully compressed, the amount of liquid discharged is equal to the required amount of the sample, so that the bellows section 22 is fully compressed with fingers to quickly remove the sample.

[0068] The above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is impossible to list all the embodiments here. Any obvious changes or modifications derived from the technical solution of the present invention are still within the scope of protection of the present invention.

Claims

1. A sampling and collecting device for intestinal flora gene detection, comprising a collection storage bottle (20) and a collection component (10), characterized in that: The collection assembly (10) comprises a suction sealing cover (11), a collection tube (12), a sealing cover (13) and a sealing sleeve (14). The suction sealing cover (11) comprises an elastic bag, a sealing joint (112), a sealing plug (114) and at least two connecting rods (113); a barb (1131) is connected to the bottom of the connecting rod (113); the connecting rod (113) is connected between the sealing plug (114) and the inner upper end of the sealing joint (112) through its two ends; the upper end of the sealing joint (112) is sealedly connected to the lower end opening of the elastic bag; the sealing joint (112) and the elastic bag are connected via a through hole (3) between the connecting rods (113); The sealing cover (13) comprises a cover body (132) and a convex tube portion (131) vertically connected to the cover body (132); a joint for detachably connecting to a sealing joint (112) is provided at the upper end of the convex tube portion (131); a sealing sleeve groove (1321) coinciding with the axis of the convex tube portion (131) is provided at the lower side of the cover body (132); a liquid outlet hole (2) is provided on the upper wall surface of the sealing sleeve groove (1321); a guide groove (1322) is provided on the outer wall surface; an installation indicator line (1323) is provided on the outer wall surface of the sealing sleeve groove (1321) near the guide groove (1322); and the cover body (132) is detachably connected to the mouth of the collection storage bottle (20); The sealing sleeve (14) is connected to the sealing sleeve slot (1321), and the liquid outlet hole (2) on the upper wall thereof coincides with the liquid outlet hole (2) on the sealing sleeve slot (1321); The collection pipe (12) comprises a straight pipe (121). The upper part of the straight pipe (121) is sealed through the sealing sleeve (14) and then plugged into the sealing cover (13). The upper end of the straight pipe (121) is provided with a hook groove (126) for hooking with the barb (1131), the lower end is provided with a collection spoon (124), and the middle part is provided with a retaining ring (123). The wall surface of the straight pipe (121) is provided with a side hole (122). The side hole (122) is located at the upper part of the retaining ring (123). The upper edge of the retaining ring (123) is vertically connected with an anti-rotation limit rod (1231) for slidingly plugging with the guide groove (1322). When the upper part of the retaining ring (123) contacts the lower part of the sealing sleeve slot (1321), the side hole (122) can overlap with the liquid outlet (2).

2. The sampling and collecting device for intestinal flora gene detection according to claim 1, characterized in that: The inner wall surface of the sealing sleeve (14) is radially provided with a plurality of convex rings.

3. The sampling and collecting device for intestinal flora gene detection according to claim 1, characterized in that: The elastic bag is a corrugated blind tube (111) integrally formed with the upper end of the sealing joint (112).

4. The sampling and collecting device for intestinal flora gene detection according to claim 3, characterized in that: A spring is connected between the corrugated blind tube (111) and the sealing joint (112).

5. The sampling and collecting device for intestinal flora gene detection according to claim 1, characterized in that: The outer wall surface of the straight tube (121) is radially provided with a sampling mark line (127).

6. The sampling and collecting device for intestinal flora gene detection according to claim 1, characterized in that: The wall surface of the convex tube portion (131) is provided with two opposite long holes (1311), and the straight tube (121) can be contacted through the long holes (1311).

7. The sampling and collecting device for intestinal flora gene detection according to claim 1, characterized in that: The lower end of the collecting spoon (124) is connected with a pointed protrusion (125).

8. The sampling and collecting device for intestinal flora gene detection according to claim 1, characterized in that: The body of the collection storage bottle (20) comprises a bellows section (22) which is integrally formed with the body and is easy to be compressed.

9. The sampling and collecting device for intestinal flora gene detection according to claim 8, characterized in that: The outer wall surface of the body of the collection storage bottle (20) comprises at least two annular grooves (21), the annular grooves (21) are connected to a C-shaped clamping ring (30), and the bellows section (22) is located between the annular grooves (21) and below the C-shaped clamping ring (30).

10. The sampling and collecting device for intestinal flora gene detection according to claim 8 or 9, characterized in that: The body of the collection storage bottle (20) is made of high-density polyethylene plastic.

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