Liquid dispensing methods and systems

Abstract

This invention provides a liquid dispensing method and system. The liquid dispensing method includes: controlling the flow of the liquid to be dispensed from the container to the dispensing module through a first transmission pipeline of a quantitative module; detecting the real-time flow rate of the sample liquid flowing into the dispensing module using the quantitative module; performing a first dispensing operation on all pre-processing units according to the real-time flow rate, and then performing a second dispensing operation on the post-processing units according to the real-time flow rate; performing a first purging process on the post-processing units; and performing a second purging process on each pre-processing unit. This invention improves the dispensing accuracy of the liquid to be dispensed while avoiding waste.

Description

Technical Field

[0001] This invention belongs to the field of biomedical technology, and in particular relates to a liquid dispensing method and system. Background Technology

[0002] Cellular formulation dispensing technology is a crucial component of the biomedical field, vital for cell therapy and pharmaceutical preparation. Failure to achieve stable and high-precision dispensing will impact the therapeutic efficacy of cell formulations and delay patient treatment. Current technologies typically measure liquid volume during dispensing by manually starting and stopping peristaltic pumps, weighing, and manually recording data. However, these methods often suffer from large residual volume in the tubing and low dispensing accuracy. The most widely used method, weighing, is particularly susceptible to interference from vibrations in the tubing and other components, leading to further issues such as large residual volume and low dispensing accuracy. Summary of the Invention

[0003] This invention addresses the technical problems of low dispensing accuracy and large residual volume in the dispensing operation of cell preparations in the prior art by providing a liquid dispensing method and system.

[0004] In view of the above technical problems, embodiments of the present invention provide a liquid dispensing method, including:

[0005] The liquid to be dispensed in the container is controlled to flow through the first transmission pipeline of the metering module to the dispensing module; the dispensing module includes at least two dispensing units; each dispensing unit includes a dispensing main pipe connected to the first transmission pipeline and at least one dispensing bag connected to the dispensing main pipe.

[0006] The real-time flow rate of the sample solution flowing into the dispensing module is detected by the quantitative module.

[0007] One post-processing unit is determined from all the packaging units. All other packaging units except the post-processing unit are recorded as pre-processing units. After performing a first packaging operation on all the pre-processing units according to the real-time traffic, a second packaging operation is performed on the post-processing unit according to the real-time traffic.

[0008] The post-processing unit is subjected to a first evacuation process to drain the liquid to be dispensed from the first transmission pipeline and the dispensing main pipe of the post-processing unit into the dispensing bag that is last dispensed in the second dispensing operation.

[0009] A second evacuation process is performed on each of the pretreatment units to drain the liquid to be dispensed from the dispensing main pipe of each pretreatment unit into the dispensing bag that is last dispensed in the first dispensing operation.

[0010] A liquid dispensing system includes a control module and a container to be dispensed, a metering module, and a dispensing module connected in sequence; the control module is connected to the metering module and the dispensing module; the control module is used to execute the liquid dispensing method.

[0011] The liquid dispensing method provided by this invention first uses a quantitative module set between the container to be dispensed and the dispensing module to detect the real-time flow rate of the liquid to be dispensed from the container to the dispensing module. Then, the liquid to be dispensed flowing into the dispensing module is precisely controlled by the measured real-time flow rate. Subsequently, the precise quantitative dispensing of the liquid to be dispensed is achieved through the first dispensing operation and the second dispensing operation. At the same time, the precise quantitative dispensing of the residual liquid to be dispensed in the pipeline is achieved through the first evacuation treatment and the second evacuation treatment. This invention improves the dispensing accuracy of the liquid to be dispensed while avoiding waste. Attached Figure Description

[0012] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0013] Figure 1 This is a schematic flowchart of the liquid dispensing method provided in the first embodiment of the present invention.

[0014] Figure 2 This is a schematic flowchart of the liquid dispensing method provided in the second embodiment of the present invention.

[0015] Figure 3 This is a schematic diagram of the structure of a liquid dispensing system provided in an embodiment of the present invention.

[0016] The reference numerals in the accompanying drawings are as follows:

[0017] 100. Processing module; 110. Container to be dispensed; 200. Quantitative module; 210. First transfer pipeline; 220. First bubble sensor; 230. Quantitative tube; 240. Second bubble sensor; 250. First liquid pump; 260. Dropper; 300. Dispensing module; 310. Dispensing unit; 311. Dispensing main pipe; 312. Dispensing bag; 313. Dispensing switch valve; 314. Dispensing bubble sensor; 315. 316. Branch pipe for dispensing; 317. Branch switch valve; 318. First sterile filter; 400. Pressure sensor; 500. Sample container; 510. Transfer module; 510. Second transfer line; 520. Second liquid pump; 530. First gas line; 540. Second sterile filter; 550. First tracheal valve; 560. First switch valve; 570. Second gas line; 580. Second tracheal valve; 590. Second switch valve. Detailed Implementation

[0018] To make the technical problems solved, the technical solutions, and the beneficial effects of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only for explaining the invention and are not intended to limit the invention.

[0019] It should be understood that the terms "upper", "lower", "left", "right", "front", "rear", "middle", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations of the present invention.

[0020] like Figure 1 and Figure 3 As shown, an embodiment of the present invention provides a liquid dispensing method, including the following steps S100-S500:

[0021] S100, the liquid to be dispensed in the container 110 is controlled to flow through the first transmission pipeline 210 of the metering module 200 to the dispensing module 300; the dispensing module 300 includes at least two dispensing units 310; each dispensing unit 310 includes a dispensing main pipe 311 connected to the first transmission pipeline 210 and at least one dispensing bag 312 connected to the dispensing main pipe 311. Wherein, Figure 3 In the embodiment shown, the container 110 to be dispensed may be part of the processing module 100.

[0022] In this embodiment, the first transmission pipeline 210 connects the container to be packaged 110 and the packaging module 300. The main packaging pipe 311 of each packaging unit 310 is connected to the first transmission pipeline 210.

[0023] Furthermore, the metering module 200 also includes a first liquid pump 250 disposed on the first transmission pipeline 210. The first liquid pump 250 is used to drive the liquid to be dispensed in the container 110 to the dispensing module 300 through the first transmission pipeline 210. The first liquid pump 250 includes a peristaltic pump. Understandably, the peristaltic pump used by the first liquid pump 250 can be determined according to the dispensing capacity of the liquid to be dispensed. For example, when the dispensing capacity of the liquid to be dispensed is small, a small peristaltic pump can be used; when the dispensing capacity of the liquid to be dispensed is large, a large peristaltic pump can be used. The peristaltic pump delivers fluid by alternately squeezing and releasing the elastic delivery hose of the peristaltic pump. The peristaltic pump can rotate forward or backward to drive the fluid (gas or liquid) to flow in different directions.

[0024] Furthermore, gas may be present in the first transmission pipeline 210 and the dispensing main pipe 311 when not in use. To prevent the gas in the first transmission pipeline 210 and the dispensing main pipe 311 from being discharged into the dispensing bag 312, a port connected to the atmospheric environment can be provided on the dispensing main pipe 311 so that the gas in the first transmission pipeline 210 and the dispensing main pipe 311 can be discharged through the port. In one embodiment, to prevent the port from affecting the flow of the liquid to be dispensed into the dispensing bag 312, the dispensing bag 312 is located between the port and the end of the dispensing main pipe 311 that connects to the first transmission pipeline 210. In one embodiment, the dispensing module 300 further includes a first sterile filter 317 disposed at the port; the first sterile filter 317 can prevent bacteria and other substances in the external atmosphere from entering the dispensing module 300.

[0025] S200, the quantitative module 200 detects the real-time flow rate of the sample liquid flowing into the dispensing module 300. Understandably, the quantitative module 200 can accurately detect the real-time flow rate of the sample liquid flowing into the dispensing module 300, which is also the real-time flow rate of the first liquid pump 250.

[0026] In one embodiment, such as Figure 3 As shown, the quantitative module 200 further includes a first bubble sensor 220, a quantitative tube 230, and a second bubble sensor 240 sequentially disposed on the first transmission pipeline 210; the first bubble sensor 220 is located between the quantitative tube 230 and the container 110 to be dispensed. It can be understood that the quantitative tube 230 can be a container with internal space or a fixed pipeline. A quantitative space exists between the first bubble sensor 220 and the second bubble sensor 240, including the internal space of the quantitative tube 230, the space between the first bubble sensor 220 and the quantitative tube 230, and the space between the quantitative tube 230 and the second bubble sensor 240. Optionally, the first liquid pump 250 is located between the container 110 to be dispensed and the first bubble sensor 220.

[0027] In one embodiment, step S200, which involves detecting the real-time flow rate of the sample solution flowing into the dispensing module 300 via the quantitative module 200, includes:

[0028] S210, the third time point at which the liquid to be dispensed flows into the metering space is detected by the first bubble sensor 220; the metering space refers to the flow space in the first transmission pipeline 210 located between the first bubble sensor 220 and the second bubble sensor 240; the metering space includes the internal space of the metering tube 230. That is, the third time point at which the liquid to be dispensed flows into the first bubble sensor 220 can be determined by the first bubble sensor 220.

[0029] S220, the fourth time point at which the liquid to be dispensed flows out of the metering space is detected by the second bubble sensor 240. That is, the fourth time point at which the liquid to be dispensed flows out of the second bubble sensor 240 can be determined by the second bubble sensor 240.

[0030] S230, obtain the spatial capacity of the quantitative space, and determine the real-time flow rate based on the spatial capacity, the third time point, and the fourth time point. That is, first, the time difference between the third time point and the fourth time point is obtained, and then the spatial capacity of the quantitative space is divided by the time difference to determine the current real-time flow rate of the first liquid pump 250. Understandably, in the above-mentioned transfer process of the liquid to be dispensed, if the liquid to be dispensed is a cell preparation, since the density and size of different cells in different liquids to be dispensed are different, the flow rate of the liquid to be dispensed in the first transfer pipeline 210 (the quotient of the real-time flow rate of the first liquid pump 250 and the cross-sectional area of ​​the first transfer pipeline 210) will affect the cell survival rate. Therefore, an initial flow rate can be preset according to the parameters of the liquid to be dispensed (including the type of liquid to be dispensed) and the parameters of the first transfer pipeline 210 (including the cross-sectional area of ​​the first transfer pipeline 210) to make the liquid to be dispensed flow at an initial speed to ensure cell activity, thereby controlling the first liquid pump 250 to operate at the initial flow rate, thereby driving the liquid to be dispensed to flow at the initial speed. However, in the actual transfer process of the liquid to be dispensed, the first liquid pump 250 cannot operate precisely at the initial flow rate, and there will inevitably be a certain error. Therefore, for the present invention, it is necessary to determine the volume of the liquid to be dispensed entering the dispensing module 300 based on the real-time flow rate. The dispensing amount of the liquid to be dispensed into each dispensing bag 312 during the dispensing process is closely related to the aforementioned volume. Therefore, it is necessary to further determine the actual change in the initial flow rate. That is, the real-time flow rate is accurately determined by the aforementioned quantitative module 200, thereby ensuring the accuracy of the dispensing amount of the liquid to be dispensed into each dispensing bag 312, thus improving dispensing accuracy. Understandably, after determining the aforementioned real-time flow rate, this real-time flow rate is fed back to the control module, replacing the initial flow rate, which facilitates retrieval during subsequent dispensing processes.

[0031] S300: A post-processing unit is determined from all the dispensing units 310. All other dispensing units 310 are recorded as pre-processing units. A first dispensing operation is performed on each of the pre-processing units according to the real-time flow. Then, a second dispensing operation is performed on the post-processing unit according to the real-time flow. It can be understood that each dispensing unit 310 includes one post-processing unit and at least one pre-processing unit. After performing the first dispensing operation on all the pre-processing units, all dispensing bags 312 except for the last dispensing bag 312 in the pre-processing units can reach a first preset dispensing capacity. The first preset dispensing capacity is the dispensing capacity that each dispensing bag 312 in the pre-processing unit will be dispensed. The first preset dispensing capacity can be set according to the user's needs within the maximum capacity limit of the dispensing bags 312. The first preset dispensing capacity corresponding to each dispensing bag 312 in the pre-processing unit can be the same or different. After performing the second dispensing operation on the post-processing unit, all dispensing bags 312 in the post-processing unit, except for the last dispensing bag 312, can reach a second preset dispensing capacity. The second preset dispensing capacity is the dispensing capacity that each dispensing bag 312 in the post-processing unit will be dispensed; the second preset dispensing capacity can be set according to user needs within the maximum capacity limit of the dispensing bags 312. The second preset dispensing capacity corresponding to each dispensing bag 312 in the post-processing unit can be the same or different. The second preset dispensing capacity can be equal to or different from the first preset dispensing capacity.

[0032] In one embodiment, each of the dispensing units 310 includes a dispensing switch valve 313 and a dispensing bubble sensor 314 mounted on the dispensing main pipe 311; all the dispensing bags 312 are located on the side of the dispensing switch valve 313 and the dispensing bubble sensor 314 away from the metering module 200. Understandably, the dispensing switch valve 313 is used to control the on / off state of the dispensing main pipe 311 on which the dispensing switch valve 313 is mounted; the dispensing bubble sensor 314 is used to detect the fluid state (e.g., whether it is gas or liquid flowing through) in the dispensing main pipe 311 on which the dispensing switch valve 313 is mounted.

[0033] In one embodiment, step S300 includes the following steps S310-S330:

[0034] S310, the dispensing switch valve 313 and dispensing bubble sensor 314 of the pre-processing unit that is currently undergoing the first dispensing operation are opened, while the other dispensing switch valves 313 remain closed. That is, the object of the first dispensing operation is the pre-processing unit, and the first dispensing operation is performed on one or more pre-processing units at a time.

[0035] S320, obtain the first preset dispensing time corresponding to the pre-processing unit, and perform first capacity error compensation on the first preset dispensing time according to the first preset time compensation rule to determine the first actual dispensing time; wherein, the product of the real-time flow rate and the first preset dispensing time is equal to the sum of the first preset dispensing capacities corresponding to each dispensing bag 312 in the pre-processing unit. It can be understood that the first preset dispensing capacity is also the dispensing capacity that each dispensing bag 312 in the pre-processing unit that needs to perform the first dispensing operation will be dispensed. The first capacity error compensation is used to compensate for pipeline capacity errors caused by pipeline manufacturing or other reasons (such as pipeline deformation caused by pump or valve clamping, etc.), and the first capacity error compensation is set according to experience in actual use. In one embodiment, the dispensing bubble sensor 314 can be directly set at the connection point between the dispensing main pipe 311 and the first transmission pipeline 210, or it can be set at a position on the dispensing main pipe 311 at a certain distance from the connection point.

[0036] S330: Obtain the first time point at which the liquid to be dispensed flows through the dispensing bubble sensor 314 of the pretreatment unit. Starting at the first time point, continue to control the real-time flow rate of the liquid to be dispensed into the dispensing main pipe 311 of the pretreatment unit. After the first actual dispensing time has elapsed, close the dispensing switch valve 313. It can be understood that after the real-time flow rate control continues at the first time point, and after the first actual dispensing time has elapsed, the volume of the liquid to be dispensed flowing into the pretreatment unit is equal to the sum of the first preset dispensing capacities corresponding to each dispensing bag 312 in the pretreatment unit. At this point, the dispensing switch valve 313 can be closed. Understandably, when the real-time flow rate is controlled to control the first actual dispensing time in the dispensing main pipe 311 of the pretreatment unit, it means that as long as all the liquid to be dispensed is dispensed into each dispensing bag 312 in the pretreatment unit, the dispensing capacity in each dispensing bag 312 will be equal to the first preset dispensing capacity corresponding to that dispensing bag 312.

[0037] In one embodiment, step S300, the second dispensing operation includes the following steps S340-S360:

[0038] S340, the dispensing switch valve 313 and dispensing bubble sensor 314 of the post-processing unit are opened, while the other dispensing switch valves 313 remain closed. That is, the object of the second dispensing operation is the post-processing unit.

[0039] S350, obtain the second preset dispensing time corresponding to the post-processing unit, and perform second capacity error compensation on the second preset dispensing time according to the second preset time compensation rule to determine the second actual dispensing time; wherein, the product of the real-time flow rate and the second preset dispensing time is equal to the difference between the total dispensing amount and the pipeline capacity of the first transmission pipeline 210, and the total dispensing amount is equal to the sum of the second preset dispensing capacities corresponding to each dispensing bag 312 in the post-processing unit. It can be understood that the second preset dispensing capacity is also the dispensing capacity that each dispensing bag 312 in the post-processing unit will be dispensed. The second capacity error compensation is used to compensate for pipeline capacity errors caused by pipeline manufacturing or other reasons (such as pipeline deformation caused by pump or valve clamping), and the first capacity error compensation is set based on experience in actual use. The second capacity error compensation may be the same as or different from the first capacity error compensation.

[0040] S360: Obtain the second time point at which the liquid to be dispensed flows through the dispensing bubble sensor 314 of the post-processing unit. Starting at the second time point, continue to control the flow of the liquid to be dispensed into the dispensing main pipe 311 of the post-processing unit at the real-time flow rate. After the second actual dispensing time has elapsed, close the dispensing switch valve 313. It can be understood that after the second time point, the real-time flow rate of the liquid to be dispensed into the dispensing main pipe 311 of the post-processing unit continues, and after the second actual dispensing time has elapsed, the volume of the liquid to be dispensed flowing into the post-processing unit is equal to the difference between the total dispensing volume and the pipe capacity of the first transmission pipeline 210. At this point, the dispensing switch valve 313 can be closed. Understandably, when the real-time flow rate is controlled to control the second actual dispensing time in the dispensing main pipe 311 of the pretreatment unit, it means that as long as all the liquid to be dispensed flowing into the post-treatment unit and all the liquid to be dispensed flowing into the first transmission pipeline 210 (equal to the pipeline capacity of the first transmission pipeline 210) are dispensed into each dispensing bag 312 in the post-treatment unit, the dispensing capacity in each dispensing bag 312 in the post-treatment unit will be equal to the second preset dispensing capacity corresponding to it.

[0041] S400, a first evacuation process is performed on the post-processing unit to drain the liquid to be dispensed from the first transmission pipeline 210 and the dispensing main pipe 311 of the post-processing unit into the dispensing bag 312, which is the last dispensing unit in the second dispensing operation. In this embodiment, after the second dispensing operation is performed on the post-processing unit, to avoid wasting the liquid to be dispensed, the first transmission pipeline 210 and the dispensing main pipe 311 of the post-processing unit can be disconnected from the dispensing container 11 when the sum of the volumes of the liquid to be dispensed in the first transmission pipeline 210 and the dispensing main pipe 311 of the post-processing unit and the liquid to be dispensed in the dispensing bag 312, which is the last dispensing unit in the second dispensing operation, equals the second preset dispensing capacity (the second preset dispensing capacity corresponding to the dispensing bag 312, which is the last dispensing unit in the second dispensing operation). The first transmission pipeline 210 is connected to the atmosphere, thereby draining the liquid to be dispensed from the first transmission pipeline 210 and the dispensing main pipe 311 of the post-processing unit into the last dispensing bag 312 to be dispensed in the second dispensing operation, avoiding waste caused by residual liquid to be dispensed in the pipeline; at the same time, after the first evacuation process, the dispensing capacity of all dispensing bags 312 in the post-processing unit except for the last dispensing bag 312 is accurate and can reach the corresponding second preset dispensing capacity.Understandably, during the second dispensing operation, the liquid to be dispensed in the first transmission pipeline 210 and the dispensing main pipe 311 of the post-processing unit may be greater than, equal to, or less than the second preset dispensing capacity corresponding to the last dispensing bag 312. When the liquid to be dispensed in the first transmission pipeline 210 and the dispensing main pipe 311 of the post-processing unit is equal to the second preset dispensing capacity corresponding to the last dispensing bag 312, the liquid to be dispensed into the last dispensing bag 312 during the second dispensing operation is 0. When the liquid to be dispensed in the first transmission pipeline 210 and the dispensing main pipe 311 of the post-processing unit is less than the second preset dispensing capacity corresponding to the last dispensing bag 312, the liquid to be dispensed into the last dispensing bag 312 during the second dispensing operation is 0. When dispensing, it is only necessary to ensure that the sum of the liquid to be dispensed in the first transmission pipeline 210 and the dispensing main pipe 311 of the post-processing unit and the liquid to be dispensed in the last dispensing bag 312 dispensed in the second dispensing operation is equal to the second preset dispensing capacity. When the liquid to be dispensed in the first transmission pipeline 210 and the dispensing main pipe 311 of the post-processing unit is greater than the second preset dispensing capacity, the number of dispensing bags 312 dispensed last in the second dispensing operation that are not full of liquid to be dispensed needs to be at least two, so as to ensure that the liquid to be dispensed in the first transmission pipeline 210 and the dispensing main pipe 311 of the post-processing unit can enter the dispensing bags 312, and that the dispensing capacity of each dispensing bag 312 after the first emptying process is equal to its corresponding second preset dispensing capacity.

[0042] S500, a second evacuation process is performed on each of the pre-processing units to drain the liquid to be dispensed from the dispensing main pipe 311 of each pre-processing unit into the last dispensing bag 312 that was dispensed in the first dispensing operation. In this embodiment, after the first dispensing operation is performed on the pre-processing unit, the dispensing capacity of all dispensing bags 312 in the pre-processing unit, except for the last dispensing bag 312, reaches their corresponding first preset dispensing capacity. In addition, after the first evacuation process is performed on the post-processing unit, the liquid to be dispensed in the first transmission pipeline 210 has also been discharged into the post-processing unit. To avoid wasting the liquid to be dispensed, the connection between the first transmission pipeline 210 and the container 110 to be dispensed can be disconnected first, and the first transmission pipeline 210 can be connected to the atmosphere. This allows the liquid to be dispensed from the dispensing main pipe 311 of the pretreatment unit to be discharged into the dispensing bag 312, which is the last dispensing bag in the first dispensing operation. This ensures that the liquid to be dispensed in the dispensing main pipe 311 of the pretreatment unit enters the dispensing bag 312, which is the last dispensing bag in the first dispensing operation. Ultimately, the dispensing capacity of the liquid to be dispensed in the dispensing bag 312 is equal to the first preset dispensing capacity. This ensures that the dispensing capacity of all the dispensing bags 312 in the pretreatment unit is equal to the first preset dispensing capacity, and also avoids waste caused by residual liquid to be dispensed in the pipeline. Understandably, during the first dispensing operation, the liquid to be dispensed in the dispensing main pipe 311 of the pretreatment unit may be greater than, equal to, or less than the first preset dispensing capacity corresponding to the last dispensing bag 312 being dispensed. When the liquid to be dispensed in the dispensing main pipe 311 of the pretreatment unit is equal to the first preset dispensing capacity corresponding to the last dispensing bag 312 being dispensed, the liquid to be dispensed into the last dispensing bag 312 during the first dispensing operation can be 0. When the liquid to be dispensed in the dispensing main pipe 311 of the pretreatment unit is less than the first preset dispensing capacity corresponding to the last dispensing bag 312 being dispensed, the liquid to be dispensed into the last dispensing bag 312 during the first dispensing operation can be 0. When dispensing, it is only necessary to ensure that the sum of the liquid to be dispensed in the dispensing main pipe 311 of the pretreatment unit and the liquid to be dispensed in the last dispensing bag 312 in the first dispensing operation is equal to the first preset dispensing capacity. When the liquid to be dispensed in the dispensing main pipe 311 of the pretreatment unit is greater than the first preset dispensing capacity corresponding to the last dispensing bag 312, the number of the last dispensing bags 312 in the first dispensing operation needs to be at least two, so as to ensure that the liquid to be dispensed in the dispensing main pipe 311 of the pretreatment unit can enter the dispensing bags 312, and that the dispensing capacity of each dispensing bag is equal to the first preset dispensing capacity after the second emptying process.

[0043] This invention first uses a quantitative module 200, located between the container 110 and the dispensing module 300, to detect the real-time flow rate of the liquid to be dispensed from the container 110 into the dispensing module 300. Then, it precisely controls the flow of the liquid into the dispensing module 300 based on the measured real-time flow rate. Subsequently, through the first and second dispensing operations, it achieves precise quantitative dispensing of the liquid to be dispensed. At the same time, through the first and second evacuation processes, it achieves precise quantitative dispensing of the residual liquid in the pipeline. This invention improves the dispensing accuracy of the liquid to be dispensed while avoiding waste.

[0044] In one embodiment, such as Figure 3 As shown, each of the dispensing units 310 includes a dispensing branch pipe 315 connecting the dispensing main pipe 311 and the dispensing bag 312, and a branch switch valve 316 installed on the dispensing branch pipe 315; all the dispensing branch pipes 315 are located on the side away from the dispensing switch valve 313 and the dispensing bubble sensor 314. That is, the dispensing bag 312 is connected to the dispensing main pipe 311 through the dispensing branch pipe 315.

[0045] In one embodiment, step S330, which involves continuing to control the flow of the liquid to be dispensed into the dispensing main pipe 311 of the pretreatment unit at the real-time flow rate starting at the first time point, and closing the dispensing switch valve 313 after the first actual dispensing time, includes:

[0046] S331, starting from the first time point, the liquid to be dispensed continues to flow into the dispensing main pipe 311 of the pretreatment unit at the real-time flow control, and continues to flow for a first preset time, so that the liquid to be dispensed flows through the connection points of the dispensing main pipe 311 of the pretreatment unit and all the dispensing branch pipes 315. It can be understood that the dispensing bag 312 is connected to the dispensing main pipe 311 through the dispensing branch pipes 315. After the first time point when the liquid to be dispensed flows through the dispensing bubble sensor 314 of the pretreatment unit, no liquid to be dispensed has yet flowed into the dispensing main pipe 311 after the dispensing bubble sensor 314. Therefore, the liquid to be dispensed is first controlled to flow through the connection points of the dispensing main pipe 311 of the pretreatment unit and each of the dispensing branch pipes 315, thereby facilitating the subsequent dispensing of each dispensing bag 312.

[0047] S332, a first liquid dispensing operation is performed sequentially on at least one of the pretreatment unit's dispensing bags 312 according to a first preset switching sequence. The first liquid dispensing operation includes: controlling the branch switch valve 316 corresponding to the dispensing bag 312 to open, and simultaneously controlling the liquid to be dispensed to flow into the dispensing bag 312 through the dispensing branch pipe 315 corresponding to the dispensing bag 312 at the real-time flow rate; after the branch switch valve 316 remains open for a second preset duration, the branch switch valve 316 is closed, so that the dispensing capacity of the dispensing bag 312 reaches the first preset dispensing capacity. It is understood that the first preset switching sequence can be set according to requirements, for example, sorted according to the distance between the dispensing bag 312 and the dispensing bubble sensor 314. Since the liquid to be dispensed in the dispensing branch pipe 315 will eventually flow into the dispensing bag 312, when performing the first liquid dispensing operation on the dispensing bag 312 of one of the pretreatment units, the product of the second preset duration and the real-time flow rate is equal to the first preset dispensing capacity. In one embodiment, a first dispensing operation can also be performed simultaneously on the dispensing bags 312 of multiple pretreatment units (at this time, the first preset dispensing capacity corresponding to the dispensing bags 312 of multiple pretreatment units should be the same). In this case, the product of a second preset duration and the real-time flow rate is equal to a first preset dispensing capacity.

[0048] S333, when it is confirmed that only one dispensing bag 312 remains in the pretreatment unit that has not undergone the first liquid separation operation, the branch switch valve 316 corresponding to the last dispensing bag 312 is opened, and the liquid to be dispensed flows into the last dispensing bag 312 at the real-time flow rate. After the branch switch valve 316 has been open for a first preset remaining time, the branch switch valve 316 and the dispensing switch valve 313 corresponding to the last dispensing bag 312 are closed. The first preset remaining time is equal to the first actual dispensing time minus the first preset time and all of the second preset times.

[0049] Understandably, in this embodiment, only the case where the liquid to be dispensed in the dispensing main pipe 311 of the pretreatment unit is equal to or less than the first preset dispensing capacity is described (therefore, in step S333, it is only confirmed that there is only one dispensing bag 312 in the pretreatment unit that has not undergone the first dispensing operation). However, referring to this embodiment, the present invention can also describe the case where the liquid to be dispensed in the dispensing main pipe 311 of the pretreatment unit is greater than the first preset dispensing capacity. In this case, it is necessary to first confirm in step S333 whether the number of dispensing bags 312 in the pretreatment unit that has not undergone the first dispensing operation is the preset number and then perform the subsequent operation (the preset number is at least two, and the preset number is equal to the minimum number of dispensing bags required to fully dispense the liquid to be dispensed in the dispensing main pipe 311 of the pretreatment unit).

[0050] In this embodiment, the first preset remaining time is equal to the first actual dispensing time minus the first preset time and all of the second preset times. Therefore, when it is confirmed that only one dispensing bag 312 remains in the pretreatment unit that has not undergone the first liquid separation operation, the branch switch valve 316 corresponding to the last dispensing bag 312 is opened. At the same time, the liquid to be dispensed flows into the last dispensing bag 312 with the real-time flow control and continues for the first preset remaining time. This indicates that the volume of the liquid to be dispensed flowing into the pretreatment unit is equal to the sum of the first preset dispensing volumes corresponding to each dispensing bag 312 in the pretreatment unit (the volume of the liquid to be dispensed flowing into the last dispensing bag and the volume of the liquid to be dispensed in the dispensing main pipe 311 of the pretreatment unit are equal to the first preset dispensing volume corresponding to the last dispensing bag, and the dispensing volumes in other dispensing bags are also equal to their corresponding first preset dispensing volumes). Therefore, at this time, the dispensing switch valve 313 can be closed.

[0051] In one embodiment, such as Figure 3 As shown, each of the dispensing units 310 includes a dispensing branch pipe 315 connecting the dispensing main pipe 311 and the dispensing bag 312, and a branch switch valve 316 installed on the dispensing branch pipe 315; all the dispensing branch pipes 315 are located on the side away from the dispensing switch valve 313 and the dispensing bubble sensor 314.

[0052] In one embodiment, step S360, which involves continuing to control the flow of the liquid to be dispensed into the dispensing main pipe 311 of the post-processing unit at the real-time flow rate starting at the second time point, and closing the dispensing switch valve 313 after the second actual dispensing time, includes:

[0053] S361, at the second time point, the liquid to be dispensed continues to flow into the dispensing main pipe 311 of the post-processing unit with the real-time flow control, and continues to flow for a third preset time period, so that the liquid to be dispensed flows through the connection points of the dispensing main pipe 311 of the post-processing unit and all the dispensing branch pipes 315. Understandably, the dispensing bag 312 is connected to the dispensing main pipe 311 via the dispensing branch pipe 315. After the dispensing liquid flows through the dispensing bubble sensor 314 of the post-processing unit at the second time point, the dispensing liquid to be dispensed has not yet flowed into the dispensing main pipe 311 after the dispensing bubble sensor 314. Therefore, by first controlling the connection points between the dispensing main pipe 311 and the dispensing branch pipe 315 of all the dispensing liquids of the post-processing units, it is possible to avoid compensating for the capacity of the dispensing main pipe 311 after the dispensing bubble sensor 314 when dispensing the dispensing bag 312. This facilitates determining the fourth preset time for the second dispensing operation of the dispensing bag 312 directly according to the second preset dispensing capacity in subsequent steps.

[0054] S362, perform a second liquid dispensing operation on at least one of the dispensing bags 312 of the post-processing unit in sequence according to the second preset switching sequence; the second liquid dispensing operation includes: controlling the branch switch valve corresponding to the dispensing bag to open, and simultaneously controlling the liquid to be dispensed to flow into the dispensing bag through the dispensing branch pipe corresponding to the dispensing bag at the real-time flow rate; after the branch switch valve is continuously open for a fourth preset time, close the branch switch valve so that the dispensing capacity of the dispensing bag reaches the second preset dispensing capacity. It is understood that the second preset switching sequence can be set according to user needs, for example, it can be sorted according to the distance between the dispensing bag 312 and the dispensing bubble sensor 314. Since the liquid to be dispensed in the dispensing branch pipe 315 will eventually flow into the dispensing bag 312, when performing the second liquid dispensing operation on the dispensing bag 312 of one of the post-processing units, the product of the fourth preset time and the real-time flow rate is equal to the second preset dispensing capacity. In one embodiment, a second liquid dispensing operation can also be performed simultaneously on the dispensing bags 312 of multiple post-processing units. In this case, the product of a fourth preset duration and the real-time flow rate is equal to a second preset dispensing capacity.

[0055] S363, when it is confirmed that only one dispensing bag 312 remains in the post-processing unit that has not undergone the second liquid separation operation, the branch switch valve 316 corresponding to the last dispensing bag 312 is opened, and the liquid to be dispensed flows into the last dispensing bag 312 at the real-time flow rate. After the branch switch valve 316 has been open for a second preset remaining time, the branch switch valve 316 and the dispensing switch valve 313 corresponding to the last dispensing bag 312 are closed. The second preset remaining time is equal to the second actual dispensing time minus the third preset time and all of the fourth preset times.

[0056] Understandably, in this embodiment, only the case where the liquid to be dispensed in the dispensing main pipe 311 of the post-processing unit is equal to or less than the second preset dispensing capacity is described (therefore, in step S363, it is only confirmed that there is only one dispensing bag 312 in the post-processing unit that has not undergone the second dispensing operation). However, referring to this embodiment, the present invention can also describe the case where the liquid to be dispensed in the dispensing main pipe 311 of the post-processing unit is greater than the second preset dispensing capacity. In this case, it is necessary to first confirm in step S363 whether the dispensing bag 312 in the pre-processing unit that has not undergone the first dispensing operation is the preset number and then perform the subsequent operation (the preset number is at least two, and the preset number is equal to the minimum number of dispensing bags required to completely dispense the liquid to be dispensed in the dispensing main pipe 311 of the post-processing unit).

[0057] In this embodiment, the second preset remaining time is equal to the second actual dispensing time minus the third preset time and all of the fourth preset times. Therefore, when it is confirmed that only one dispensing bag 312 remains in the post-processing unit that has not undergone the second liquid dispensing operation, the branch switch valve 316 corresponding to the last dispensing bag 312 is opened, and the liquid to be dispensed flows into the last dispensing bag 312 with the real-time flow control. After the branch switch valve 316 has been open for the second preset remaining time, it indicates that the sum of the volume of the liquid to be dispensed flowing into the post-processing unit and the volume of the liquid to be dispensed in the first transmission pipeline 210 is equal to the total dispensing volume required by the post-processing unit (that is, the sum of the first preset dispensing volumes corresponding to each dispensing bag 312 in the post-processing unit). Therefore, at this time, the dispensing switch valve 313 can be closed.

[0058] Furthermore, after step S500, that is, after performing the second emptying process on each of the preprocessing units, the method further includes:

[0059] S600, the branch switch valves 316 of the post-processing unit and all the pre-processing units are opened, so that the liquid to be dispensed in each of the dispensing branch pipes 315 flows into the dispensing bags 312 corresponding to the branch switch valves 316. It can be understood that opening the branch switch valves 316 on the dispensing branch pipes 315 allows the liquid to be dispensed in the dispensing branch pipes 315 to flow into the dispensing bags 312 corresponding to the branch switch valves 316, thus avoiding waste of the liquid while ensuring accurate dispensing. Furthermore, in this embodiment, opening all the branch switch valves 316 also allows all the dispensing bags 312 to be connected to the atmospheric environment through the dispensing branch pipes 315 and the dispensing main pipe 311, thereby balancing the air pressure in all the dispensing bags 312.

[0060] In one embodiment, each of the dispensing units 310 includes a pressure sensor mounted on the dispensing main pipe 311 for detecting the air pressure of the dispensing main pipe 311.

[0061] Step S600, after opening the branch switch valves 316 of the post-processing unit and all the pre-processing units to allow the liquid to be dispensed in each of the dispensing branch pipes 315 to flow into the dispensing bags 312 corresponding to the branch switch valves 316, further includes:

[0062] S700, close all branch switch valves 316. That is, in step S600, after the liquid to be dispensed in all the dispensing branch pipes 315 has flowed into the dispensing bags 312 corresponding to the branch switch valves 316, and the air pressure in all the dispensing bags 312 has been equalized, all the branch switch valves 316 can be closed.

[0063] S800, according to a preset evacuation sequence, evacuation operations are performed on each of the dispensing bags 312 in the post-processing unit and all the pre-processing units. The evacuation operation includes: opening the branch switch valve 316 corresponding to the dispensing bag 312, controlling the gas in the dispensing bag 312 to be discharged through the dispensing branch pipe 315, the dispensing main pipe 311, and the first transmission pipe 210; simultaneously acquiring the real-time pressure value inside the dispensing main pipe 311 measured by the pressure sensor corresponding to the opened branch switch valve 316; and confirming the completion of the evacuation operation for the dispensing bag 312 when the real-time pressure value inside the pipe reaches a preset pressure threshold (the preset pressure threshold is set according to requirements). It can be understood that the preset evacuation sequence can be arranged according to the distance between the dispensing bag 312 and the dispensing bubble sensor 314, or it can be set according to user requirements. The branch valves 316 corresponding to each dispensing bag 312 are opened sequentially according to a preset evacuation sequence to evacuate each dispensing bag 312, thereby achieving precise evacuation and improving the dispensing effect. A pressure sensor monitors the real-time pressure value inside the dispensing main pipe 311. When the real-time pressure value reaches a preset pressure threshold, it indicates that the gas in the dispensing bag 312 has been evacuated. At this point, the branch valve 316 corresponding to that dispensing bag 312 can be closed, and the evacuation operation can be performed on the next dispensing bag 312 according to the preset evacuation sequence. In this way, the gas in each dispensing bag 312 can be evacuated sequentially.

[0064] S900, when the evacuation operation of all the dispensing bags 312 is completed, the dispensing is confirmed to be finished. That is, after the last dispensing bag 312 determined according to the preset evacuation sequence has completed the evacuation operation, it indicates that the dispensing of the liquid to be dispensed in all dispensing bags 312 has been completed. At this time, the next round of dispensing can be carried out. In this case, the next round of dispensing pipeline can be switched by replacing the first transfer pipeline 210, the dispensing main pipeline 311, the dispensing branch pipeline 315, and the dispensing bag 312, or the next round of dispensing can be carried out by directly replacing the dispensing bag 312. Specifically, the pipeline can be cut off by heat sealing, and then the replaced pipeline can be heat sealed and connected by a sterile connection machine.

[0065] In one embodiment, before step S100, which involves controlling the liquid to be dispensed in the container 110 to flow through the first transmission pipeline 210 of the metering module 200 to the dispensing module 300, the method further includes steps S1000-S1100:

[0066] S1000: After the sample liquid in the container 110 to be dispensed is cooled by the cooling module, the sample liquid of a preset capacity in the sample container 400 is controlled to flow to the container 110 to be dispensed through the second transmission pipe 510 of the transmission module 500; the end of the first transmission pipe 210 away from the dispensing module 300 is connected to the second transmission pipe 510. The sample container 400 can be a liquid bag or other container used to hold sample liquids (such as various cell preparations that need to be dispensed). Understandably, before dispensing the sample liquid, the sample container 400 and the container 110 to be dispensed must first be connected so that the sample liquid flows from the sample container 400 into the container 110 to be dispensed. The preset capacity is greater than or equal to the total capacity of the liquid to be dispensed into the dispensing module 300 (i.e., into all dispensing bags 312 of the dispensing module 300), thereby ensuring that the liquid to be dispensed can be dispensed into all dispensing bags 312 of the dispensing module 300 and meet the dispensing capacity requirements.

[0067] Furthermore, such as Figure 3 As shown, the transmission module 500 further includes a second liquid pump 520 disposed on the second transmission pipeline 510. The second liquid pump 520 is used to drive the sample liquid in the sample container 400 to the dispensing container 110 through the second transmission pipeline 510. The preset capacity can be determined by the flow rate of the second liquid pump 520 and the operating time of the second liquid pump 520. Therefore, after confirming that the volume of the dispensing liquid in the dispensing container 110 has reached the preset capacity based on the operating time, the second liquid pump 520 will be controlled to shut down to stop the transfer of sample liquid from the sample container 400 to the dispensing container 110. The second liquid pump 520 includes a peristaltic pump. It can be understood that the peristaltic pump used by the second liquid pump 520 can be determined according to the dispensing capacity of the sample liquid to be transferred. For example, when the volume of the sample liquid to be transferred is small, a small peristaltic pump can be used; when the volume of the sample liquid to be transferred is large, a large peristaltic pump can be used. The peristaltic pump delivers fluid by alternately squeezing and releasing the elastic delivery hose of the peristaltic pump. Peristaltic pumps can rotate in either direction to drive fluids (gas or liquid) to flow in different directions.

[0068] Furthermore, the refrigeration module includes a refrigeration component, a temperature control sensor, and a refrigeration plate that is attached to the container 110 to be dispensed; the refrigeration module is provided with a first refrigeration space for accommodating the refrigeration plate and the container 110 to be dispensed. Both the refrigeration module and the mixing module can be part of the processing module 100.

[0069] After the sample liquid in the container 110 is cooled by the cooling module, the sample liquid of a preset capacity in the sample container 400 is controlled to flow to the container 110 through the second transmission pipeline 510 of the transmission module 500, including:

[0070] The refrigeration module is controlled to cool the first refrigeration space and the refrigeration plate according to the first refrigeration parameters. The refrigeration plate in the refrigeration module, which is attached to the container 110 to be dispensed, provides centralized refrigeration. An insulation layer is provided between the refrigeration plate and other surrounding components to prevent energy loss and maintain the temperature of the container 110. In other words, during liquid dispensing, the refrigeration module needs to cool the first refrigeration space containing the container 110 to bring it to the required temperature range. To achieve a rapid temperature drop, a refrigeration plate is attached to the container 110 for centralized refrigeration. The first refrigeration parameters can be set as needed and may include cooling parameters for the refrigeration module to cool the first refrigeration space and the refrigeration plate, such as the required temperature and cooling duration for each.

[0071] A temperature set containing at least one real-time temperature is obtained by detecting the temperature control sensor. After the temperature set meets the preset temperature requirement, the sample liquid of a preset volume in the sample container 400 is controlled to flow through the second transmission pipe 510 of the transmission module 500 to the dispensing container 110. The temperature control sensor includes at least one first temperature sensor disposed in the first cooling space and a second temperature sensor disposed on the cooling plate. The real-time temperature includes a first real-time temperature measured by any one of the first temperature sensors and a second real-time temperature measured by the second temperature sensor disposed on the cooling plate. In this embodiment, the temperature set may only include the first real-time temperature measured by the first temperature sensor in the first cooling space, or only the second real-time temperature measured by the second temperature sensor; or it may include both the second real-time temperature and one or more first real-time temperatures, as long as all real-time temperatures in the temperature set meet the preset temperature requirement. Meeting the preset temperature requirement can mean that each real-time temperature in the temperature set reaches its corresponding cooling temperature requirement, or it can mean that the average value of all real-time temperatures reaches its corresponding cooling temperature requirement. In other words, the cooling module performs cooling treatment according to the first cooling parameters, reducing the temperature in the first cooling space and / or the temperature of the cooling plate in the cooling module to the required temperature set in the first cooling parameters, so as to meet the preset temperature requirements. The preset temperature requirements may refer to the required temperature range (e.g., 1-25 degrees Celsius) for storing the sample solution. The first cooling parameters are set according to the above-mentioned preset temperature requirements. In this invention, if the sample solution is a cell preparation, it often needs to be stored within the required temperature range to avoid damaging the integrity and activity of the cell preparation. Understandably, the temperature is monitored in real time by a temperature control sensor in the cooling module. The cooling components of the cooling module may include components such as a compressor and an evaporator. The specific cooling method can be referred to as the cooling method of an air conditioner, etc., and will not be elaborated here.

[0072] In one embodiment, the refrigeration module further includes a second refrigeration space for accommodating the dispensing module 300 and communicating with the first refrigeration space; the refrigeration module also includes a temperature transmission component disposed between the first refrigeration space and the second refrigeration space; further, after the sample liquid of a preset capacity in the controlled sample container 400 flows through the second transmission pipe 510 of the transmission module 500 to the dispensing container 110, the module further includes:

[0073] Obtain the second cooling parameters; the second cooling parameters can be set according to the requirements, and can be considered as the cooling parameters for cooling the second cooling space, such as the temperature to which it needs to be reduced and the cooling time.

[0074] According to the second cooling parameters, the temperature transmission component controls the first cooling space and the second cooling space to exchange heat, thereby performing a second cooling process on the second cooling space used to accommodate the dispensing module 300. That is, in this embodiment, the dispensing module 300 is located in the second cooling space, and the temperature in the second cooling space also needs to meet the same preset temperature requirements as the first cooling space. Therefore, a temperature transmission component can be set to exchange heat between the first cooling space and the second cooling space. For example, the temperature transmission component may include an air duct connecting the first cooling space and the second cooling space and a fan installed in the air duct. Then, the fan can drive the cold air in the first cooling space into the second cooling space to cool the second cooling space, thereby making it also meet the preset temperature requirements corresponding to the second cooling space. It is understood that in this invention, the time for performing the second cooling process can be controlled before the sample liquid in the container to be dispensed 110 flows to the dispensing module 300. However, the second cooling process can also be performed during the process of controlling the sample liquid in the container to be dispensed 110 to flow to the dispensing module 300. However, in another embodiment, an additional refrigeration module can be installed in the second refrigeration space to cool it down, thereby rapidly cooling the second refrigeration space to meet the preset temperature requirement.

[0075] S1100, the sample liquid in the container 110 to be dispensed is mixed by the mixing module to form the liquid to be dispensed. The mixing module may be part of the processing module 100.

[0076] The aforementioned mixing process refers to mixing the sample liquid in the container 110 according to pre-set mixing parameters (including, but not limited to, the mixing frequency, mixing amplitude, mixing speed, and mixing acceleration of the mixing component driving the mixing plate to squeeze or tap the container 110 to be dispensed). The mixing module includes a mixing plate (not shown) and a mixing component (not shown) connected to the mixing plate; the mixing plate can be positioned opposite the cooling plate; the container 110 to be dispensed is located between the mixing plate and the cooling plate; in this case, the mixing plate squeezes or taps the container 110 placed on the cooling plate, causing the sample liquid in the container 110 to flow upwards and then sink, thus simultaneously cooling the sample liquid in the container 110 and mixing it.

[0077] In one embodiment, such as Figure 3As shown, the transmission module 500 further includes a first gas pipeline 530 connected to the second transmission pipeline 510, a second sterile filter 540 disposed at one end of the first gas pipeline 530 away from the second transmission pipeline 510, a first gas valve 550 disposed on the first gas pipeline 530, and a first switching valve 560 disposed on the second transmission pipeline 510 and located between the connection point of the first gas pipeline 530 and the second transmission pipeline 510 and the sample container 400; the second sterile filter 540 can prevent bacteria and other substances in the outside atmosphere from entering all pipelines of the transmission module 500.

[0078] Step S1000, after the sample liquid of a preset volume in the controlled sample container 400 flows through the second transmission pipe 510 of the transmission module 500 to the dispensing container 110, further includes:

[0079] S1100, when the volume of sample liquid entering the dispensing container 110 exceeds a preset proportion of the preset capacity, an venting process is performed. This venting process involves disconnecting the second transfer pipe 510 from the sample container 400 and driving the gas in the dispensing container 110 through the first gas pipe 530 via the second liquid pump 520, thereby venting it from the second sterile filter 540. The preset proportion can be set according to requirements, for example, including but not limited to setting it to two-thirds of the preset capacity. That is, at this time, when the volume of liquid to be dispensed from the sample container 400 to the dispensing container 110 exceeds a preset proportion of the preset capacity of the dispensing container 110, it is necessary to partially or completely vent the air in the dispensing container 110 to prevent the dispensing container 110 from bursting during subsequent dispensing of the liquid from the sample container 400 to the dispensing container 110. Therefore, at this time, if... Figure 3 As shown, the first switch valve 560 can be closed first, so that the sample liquid in the sample container 400 will not flow into the dispensing container 110 from the second transfer line 510. Then, the first gas valve 550 is opened, and the gas in the dispensing container 110 is driven by the second liquid pump 520 to flow along the second transfer line 510 through the first gas line 530 and out of the second sterile filter 540.

[0080] In one embodiment, such as Figure 3As shown, the quantitative module 200 further includes a second gas pipeline 570 connected to the first transmission pipeline 210 and located between the connection point of the first transmission pipeline 210 and the second transmission pipeline 510 and the first liquid pump 250; a second gas valve 580 disposed on the second gas pipeline 570; and a second switching valve 590 disposed on the first transmission pipeline 210 and located between the connection point of the first transmission pipeline 210 and the second transmission pipeline 510 and the connection point of the first transmission pipeline 210 and the second gas pipeline 570. One end of the second gas pipeline 570 away from the first transmission pipeline 210 is connected to the first gas pipeline 530 to allow connection to the atmospheric environment. In one embodiment, the end of the second gas pipeline 570 away from the first transmission pipeline 210 can also be directly connected to the atmospheric environment, and a third sterile filter is provided at the end of the second gas pipeline 570 away from the first transmission pipeline 210 to prevent bacteria and other contaminants from the external atmosphere from entering all pipelines of the quantitative module 200.

[0081] In step S400, when the post-processing unit undergoes a first venting process, and in step S500, when the pre-processing unit undergoes a second venting process, the second switching valve 590 can be closed first to prevent gas from flowing from the first transmission line 210 into the dispensing container 110. Then, the second gas valve 580 is opened, allowing the gas from the first and second venting processes to be discharged from the second sterile filter 540 along the first transmission line 210, through the second gas line 570 and the first gas line 530.

[0082] It should be understood that the sequence number of each step in the above embodiments does not imply the order of execution. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present invention.

[0083] like Figure 3 As shown, an embodiment of the present invention also provides a liquid dispensing system for implementing the above-described liquid dispensing method. The liquid dispensing system includes a control module and a container 110 to be dispensed, a metering module 200, and a dispensing module 300 connected in sequence. In one embodiment, the liquid dispensing system includes a processing module 100, and the processing module 100 includes a container 110 for containing the liquid to be dispensed. The processing module 110 may also include the above-described mixing module and cooling module, etc.

[0084] The quantitative module 200 includes a first transmission pipeline 210 connecting the container to be dispensed 110 and the dispensing module 300, and a first liquid pump 250 disposed on the first transmission pipeline 210 and used to drive the flow of fluid in the first transmission pipeline 210.

[0085] The packaging module 300 includes at least two packaging units 310; each packaging unit 310 includes a post-processing unit and at least one pre-processing unit; each packaging unit 310 includes a main packaging pipe 311 connected to the first transmission pipeline 210 and at least one packaging bag 312 connected to the main packaging pipe 311.

[0086] In one embodiment, such as Figure 3 As shown, the liquid dispensing system further includes: a sample container 400, and a transfer module 500 for conveying a sample liquid of a preset capacity in the sample container 400 to the container to be dispensed 110.

[0087] The transmission module 500 includes a second transmission pipeline 510 connecting the sample container 400 and the container to be dispensed 110, and a second liquid pump 520 disposed on the second transmission pipeline 510 for driving the flow of fluid in the second transmission pipeline 510; one end of the first transmission pipeline 210 away from the dispensing module 300 is connected to the second transmission pipeline 510.

[0088] In one embodiment, such as Figure 3 As shown, the quantitative module 200 further includes a dripping funnel 260 disposed on the first transmission pipeline 210; the dripping funnel 260 is disposed on the first transmission pipeline 210 and located between the first bubble sensor 220 and the container to be dispensed 110; the dripping funnel 260 is used to eliminate air bubbles in the liquid to be dispensed entering the dispensing module 300.

[0089] The specific settings of the control module and other modules of the liquid dispensing system correspond one-to-one with the liquid dispensing method described above, and will not be repeated here. Each module in the control module can be implemented entirely or partially through software, hardware, or a combination thereof. These modules can be embedded in the control module in hardware form or independent of it, or stored in the control module in software form, so that the control module can call and execute the operations corresponding to each module.

[0090] An embodiment of the present invention also provides a computer-readable storage medium storing a computer program, characterized in that the computer program, when executed by a processor, implements the liquid dispensing method.

[0091] Those skilled in the art will understand that all or part of the processes in the methods of the above embodiments can be implemented by instructing related hardware with computer-readable instructions. These computer-readable instructions can be stored in a non-volatile readable storage medium or a volatile readable storage medium. When executed, these computer-readable instructions can include the processes of the embodiments of the above methods. Any references to memory, storage, databases, or other media used in the embodiments provided in this application can include non-volatile and / or volatile memory. Non-volatile memory may include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM), or flash memory. Volatile memory may include random access memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in a variety of forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), dual data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous link DRAM (SLDRAM), RAMbus direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM).

[0092] Those skilled in the art will clearly understand that, for the sake of convenience and brevity, the above-described division of functional units and modules is used as an example. In practical applications, the above functions can be assigned to different functional units and modules as needed, that is, the internal structure of the system can be divided into different functional units or modules to complete all or part of the functions described above.

[0093] The above are merely embodiments of the liquid dispensing method and system of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A liquid dispensing method, characterized in that, include: The liquid to be dispensed in the container is controlled to flow through the first transmission pipeline of the metering module to the dispensing module; the dispensing module includes at least two dispensing units; each dispensing unit includes a dispensing main pipe connected to the first transmission pipeline and at least one dispensing bag connected to the dispensing main pipe. The real-time flow rate of the sample solution flowing into the dispensing module is detected by the quantitative module. One post-processing unit is determined from all the packaging units. All other packaging units except the post-processing unit are recorded as pre-processing units. After performing a first packaging operation on all the pre-processing units according to the real-time traffic, a second packaging operation is performed on the post-processing unit according to the real-time traffic. The post-processing unit is subjected to a first evacuation process to drain the liquid to be dispensed from the first transmission pipeline and the dispensing main pipe of the post-processing unit into the dispensing bag that is last dispensed in the second dispensing operation. A second evacuation process is performed on each of the pretreatment units to drain the liquid to be dispensed from the dispensing main pipe of each pretreatment unit into the dispensing bag that is last dispensed in the first dispensing operation.

2. The liquid dispensing method according to claim 1, characterized in that, Each of the dispensing units includes a dispensing switch valve and a dispensing bubble sensor mounted on the dispensing main pipe; all the dispensing bags are located on the side of the dispensing switch valve and the dispensing bubble sensor away from the metering module; The first dispensing operation includes: Turn on the dispensing switch valve and dispensing bubble sensor of the pre-processing unit that needs to perform the first dispensing operation, and keep the other dispensing switch valves closed; The first preset dispensing time corresponding to the preprocessing unit is obtained, and the first preset dispensing time is compensated for a first capacity error according to the first preset time compensation rule to determine the first actual dispensing time; wherein, the product of the real-time flow rate and the first preset dispensing time is equal to the sum of the first preset dispensing capacities corresponding to each dispensing bag in the preprocessing unit. The first time point of the liquid to be dispensed is obtained when it flows through the dispensing bubble sensor of the pretreatment unit. At the first time point, the liquid to be dispensed continues to flow into the dispensing main pipe of the pretreatment unit with the real-time flow control. After the first actual dispensing time has elapsed, the dispensing switch valve is closed.

3. The liquid dispensing method according to claim 2, characterized in that, Each of the dispensing units includes a dispensing branch pipe connecting the dispensing main pipe and the dispensing bag, and a branch switch valve installed on the dispensing branch pipe; The step of continuing to control the flow of the liquid to be dispensed into the dispensing main pipe of the pretreatment unit at the real-time flow rate starting at the first time point, and closing the dispensing switch valve after the first actual dispensing time, includes: Starting at the first time point, the liquid to be dispensed continues to flow into the dispensing main pipe of the pretreatment unit at the real-time flow control, and continues to flow for a first preset time, so that the liquid to be dispensed flows through the connection points of the dispensing main pipe of the pretreatment unit and all the dispensing branch pipes. The first liquid dispensing operation is performed on the dispensing bag of the pretreatment unit in sequence according to the first preset switch sequence; the first liquid dispensing operation includes: controlling the branch switch valve corresponding to the dispensing bag to open, and controlling the liquid to be dispensed to flow into the dispensing bag through the dispensing branch pipe corresponding to the dispensing bag with the real-time flow control; after the branch switch valve is continuously open for a second preset time, the branch switch valve is closed so that the dispensing capacity of the dispensing bag reaches the first preset dispensing capacity. When it is confirmed that only one dispensing bag remains in the pretreatment unit that has not undergone the first liquid separation operation, the branch switch valve corresponding to the last dispensing bag is opened, and the liquid to be dispensed flows into the last dispensing bag at the real-time flow rate. After the branch switch valve remains open for a first preset remaining time, the branch switch valve corresponding to the last dispensing bag and the dispensing switch valve are closed. The first preset remaining time is equal to the first actual dispensing time minus the first preset time and all of the second preset times.

4. The liquid dispensing method according to claim 1, characterized in that, Each of the dispensing units includes a dispensing switch valve and a dispensing bubble sensor mounted on the dispensing main pipe; all the dispensing bags are located on the side of the dispensing switch valve and the dispensing bubble sensor away from the metering module; The second dispensing operation includes: Open the dispensing switch valve and dispensing bubble sensor of the post-processing unit, and keep the other dispensing switch valves closed; The second preset dispensing time corresponding to the post-processing unit is obtained, and the second preset dispensing time is compensated for second capacity error according to the second preset time compensation rule to determine the second actual dispensing time; wherein, the product of the real-time flow rate and the second preset dispensing time is equal to the difference between the total dispensing amount and the pipeline capacity of the first transmission pipeline, and the total dispensing amount is equal to the sum of the second preset dispensing capacities corresponding to each dispensing bag in the post-processing unit; The second time point is obtained when the liquid to be dispensed flows through the dispensing bubble sensor of the post-processing unit. At the second time point, the liquid to be dispensed continues to flow into the dispensing main pipe of the post-processing unit with the real-time flow control. After the second actual dispensing time has elapsed, the dispensing switch valve is closed.

5. The liquid dispensing method according to claim 4, characterized in that, Each of the dispensing units includes a dispensing branch pipe connecting the dispensing main pipe and the dispensing bag, and a branch switch valve installed on the dispensing branch pipe; The step of continuing to control the flow of the liquid to be dispensed into the dispensing main pipe of the post-processing unit at the real-time flow rate starting at the second time point, and closing the dispensing switch valve after the second actual dispensing time, includes: Starting at the second time point, the liquid to be dispensed continues to flow into the dispensing main pipe of the post-processing unit at the real-time flow control, and continues to flow for a third preset time, so that the liquid to be dispensed flows through the connection points of the dispensing main pipe of the post-processing unit and all the dispensing branch pipes. The second liquid dispensing operation is performed on the dispensing bag of the post-processing unit in the second preset switching sequence. The second liquid dispensing operation includes: controlling the branch switch valve corresponding to the dispensing bag to open, and controlling the liquid to be dispensed to flow into the dispensing bag through the dispensing branch pipe corresponding to the dispensing bag with the real-time flow control. After the branch switch valve is continuously open for a fourth preset time, the branch switch valve is closed so that the dispensing capacity of the dispensing bag reaches the second preset dispensing capacity. When it is confirmed that only one dispensing bag remains in the post-processing unit that has not undergone the second dispensing operation, the branch switch valve corresponding to the last dispensing bag is opened, and the dispensing liquid is controlled to flow into the last dispensing bag at the real-time flow rate. After the branch switch valve remains open for a second preset remaining time, the branch switch valve corresponding to the last dispensing bag and the dispensing switch valve are closed. The second preset remaining time is equal to the second actual dispensing time minus the third preset time and all of the fourth preset times.

6. The liquid dispensing method according to claim 3 or 5, characterized in that, After performing the second emptying process on each of the preprocessing units, the method further includes: Open the branch switch valves of the post-processing unit and all the pre-processing units so that the liquid to be dispensed in each of the dispensing branch pipes flows into the dispensing bag corresponding to the branch switch valve.

7. The liquid dispensing method according to claim 6, characterized in that, Each of the dispensing units includes a pressure sensor mounted on the dispensing main pipe for detecting the air pressure of the dispensing main pipe; After opening the branch switch valves of the post-processing unit and all the pre-processing units to allow the liquid to be dispensed in each of the dispensing branch pipes to flow into the dispensing bag corresponding to the branch switch valve, the process further includes: Close all of the aforementioned branch switch valves; The post-processing unit and all the pre-processing units are sequentially evacuated according to a preset evacuation sequence. The evacuation operation includes: opening the branch switch valve corresponding to the evacuation bag, controlling the gas in the evacuation bag to be discharged through the evacuation branch pipe, the evacuation main pipe and the first transmission pipeline, and simultaneously acquiring the real-time pressure value in the evacuation main pipe measured by the pressure sensor corresponding to the opened branch switch valve. When the real-time pressure value in the evacuation pipe reaches a preset pressure threshold, the evacuation operation of the evacuation bag is confirmed to be completed. Once the air extraction operation of all the described dispensing bags is completed, confirm that the dispensing is finished.

8. The liquid dispensing method according to claim 1, characterized in that, The quantitative module further includes a first bubble sensor, a quantitative tube, and a second bubble sensor sequentially arranged on the first transmission pipeline; the first bubble sensor is located between the quantitative tube and the container to be dispensed. The step of detecting the real-time flow rate of the sample solution flowing into the dispensing module via the quantitative module includes: The first bubble sensor detects the third time point at which the liquid to be dispensed flows into the metering space; the metering space refers to the flow space in the first transmission pipeline located between the first bubble sensor and the second bubble sensor; the metering space includes the internal space of the metering tube; The fourth time point at which the liquid to be dispensed flows out of the quantitative space is detected by the second bubble sensor; Obtain the spatial capacity of the quantitative space, and determine the real-time flow rate based on the spatial capacity, the third time point, and the fourth time point.

9. The liquid dispensing method according to claim 1, characterized in that, Before the liquid to be dispensed in the container flows through the first transfer pipeline of the metering module to the dispensing module, the method further includes: After the sample liquid in the container to be dispensed is cooled by the cooling module, the sample liquid of a preset volume in the sample container is controlled to flow to the container to be dispensed through the second transmission pipeline of the transmission module; the end of the first transmission pipeline away from the dispensing module is connected to the second transmission pipeline. The sample liquid in the container to be dispensed is mixed by the mixing module to form the liquid to be dispensed.

10. A liquid dispensing system, characterized in that, The device includes a control module and a container to be dispensed, a metering module, and a dispensing module connected in sequence; the control module is connected to the metering module and the dispensing module; the control module is used to execute the liquid dispensing method as described in any one of claims 1 to 9.

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