ATP (adenosine triphosphate) immobilized enzyme catalytic synthesis device and synthesis process

Abstract

The invention relates to the technical field of immobilized enzyme catalysis, in particular to an ATP immobilized enzyme catalytic synthesis device and a synthesis technology.The outer wall of a catalysis box is fixedly connected with a reaction liquid containing box, and the side, away from the reaction liquid containing box, of the outer wall of the catalysis box is fixedly connected with a second connecting pipe; according to the ATP immobilized enzyme catalytic synthesis device and the synthesis process, the reaction box is an inclined box body with a gradient, so that the situation that the catalytic efficiency cannot be improved due to the fact that the activity of enzyme is reduced and the catalytic efficiency cannot be improved due to the fact that reaction liquid is in contact with the enzyme for a long time after entering the box is avoided; after the reaction liquid enters the reaction box through the second interface, a rotating column is arranged on the inner wall of the reaction box and drives rotating blades in a circumferential array to rotate, a plurality of placing discs are arranged on the outer wall of the rotating column, round holes are formed in the surfaces of the placing discs, and the porous carrier is easy for the reaction liquid to diffuse to activity sites of enzyme more easily; the catalytic efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of immobilized enzyme catalysis, and particularly to an ATP immobilized enzyme catalytic synthesis device and a synthesis process. Background Art

[0002] In the pharmaceutical field, immobilized enzymes can improve the efficiency and purity of drug production, reduce costs, and achieve directional synthesis and fine control of drugs. For example, immobilized penicillin acylase is used to manufacture various semi-synthetic penicillins and cephalosporins. In the food industry, immobilized enzymes can be used to improve food quality. For example, immobilized tannase and β-glucosidase are used to remove turbidity and enhance the aroma of tea beverages. In the energy field, immobilized enzymes can be applied to the production of biofuels to improve conversion efficiency and stability and promote the development of sustainable energy. In environmental governance, immobilized enzymes can be used for wastewater treatment and pollutant degradation to reduce the harm of wastewater to the environment, etc. In the existing immobilized enzyme process, after the reaction liquid enters the box, the activity of the enzyme is reduced due to long-term contact with the enzyme, thus unable to improve the catalytic efficiency. Moreover, the catalytic efficiency of the immobilized enzyme is related to the properties of the carrier. Different carrier materials, particle sizes, and surface areas of the carrier will all affect the contact degree between the substrate and the enzyme. Therefore, we propose an ATP immobilized enzyme catalytic synthesis device and a synthesis process. Summary of the Invention

[0003] The present invention provides the following technical solution: An ATP immobilized enzyme catalytic synthesis device includes a catalytic box. The outer wall of the catalytic box is fixedly connected with a reaction liquid placement box. The outer wall of the catalytic box is fixedly connected with a second connecting pipe on the side far from the reaction liquid placement box. The outer wall of the second connecting pipe is fixedly connected with a second interface. A high-concentration liquid box is provided on the outer wall of the catalytic box on the side far from the reaction liquid placement box. The outer wall of the high-concentration liquid box is fixedly connected with a first connecting pipe. The outer wall of the first connecting pipe is fixedly connected with a first interface. A reaction box is provided inside the catalytic box, and a placement component is provided inside the reaction box. The placement component includes a fixed block located on the inner wall of the reaction box. A driving motor is provided on the outer wall of the fixed block. A rotating column is provided at the other end of the outer wall of the fixed block. Rotating blades are provided on the outer wall of the rotating column. A connecting long rod is provided on the outer wall of the rotating column. A plurality of placement disks are provided on the outer wall of the connecting long rod. The plurality of placement disks are linearly and arrayedly distributed on the outer wall of the connecting long rod. Circular holes are provided on the outer walls of the plurality of placement disks. A button is provided on the inner wall of the reaction box. The button and the rotating blades are on the same horizontal line. A first sliding groove is provided on the inner wall of the reaction box. A first sliding plate is slidably connected to the outer wall of the first sliding groove. A second sliding groove is provided on the inner wall of the reaction box on the side close to the first sliding groove. A second sliding plate is slidably connected to the outer wall of the second sliding groove.

[0004] As a preferred technical solution of the present invention, the first sliding plate and the second sliding plate are respectively located at the left and right ends of the inner wall of the reaction tank, and the first sliding plate and the second sliding plate slide in opposite directions.

[0005] As a preferred technical solution of the present invention, an activity component is provided on the outer wall of the reaction tank. The activity component includes an activity cavity located on the outer wall of the reaction tank. A piston is provided on the inner wall of the activity cavity. A connecting rod is fixedly connected to the outer wall of the piston, and a pointer is provided on the outer wall of the connecting rod.

[0006] As a preferred technical solution of the present invention, a monitoring component is provided on the outer wall of the connecting rod. The monitoring component includes a speed measurement box located on the outer wall of the connecting rod. A pressing rod is provided on the outer wall of the speed measurement box, and a connecting block is fixedly connected to the outer wall of the speed measurement box.

[0007] As a preferred technical solution of the present invention, the driving motor is electrically connected to the monitoring component.

[0008] As a preferred technical solution of the present invention, the outer wall of the pointer is in contact with the outer wall of the pressing rod.

[0009] As a preferred technical solution of the present invention, the rotation speed of the rotating blade is different from the rotation speed of the connecting long rod.

[0010] As a preferred technical solution of the present invention, an outlet is provided on the outer wall of the catalytic box, a placement groove is provided on the top of the catalytic box, and a T-shaped guide plate is fixedly connected to the inner wall of the reaction tank.

[0011] An ATP immobilized enzyme catalyzed synthesis process, which includes the following processes: S1: The reaction solution enters the inner wall of the reaction tank through the second connecting pipe. The reaction tank is set as an inclined plate with a slope. When the liquid enters the reaction tank, it flows from a high place to a low place to the bottom of the reaction tank and then flows out from the outlet; S2: When the reaction solution enters the reaction tank, since the inner wall is provided with rotating blades, the rotating blades push the liquid to flow, preventing sedimentation and accumulation, and ensuring that the placement discs provided on the outer wall of the connecting long rod are fully in contact with the reaction solution when rotating; S3: A button is provided at the bottom of the rotating blade. When the rotating blade rotates, the blade presses the button, so that the first sliding plate and the second sliding plate slide in the sliding groove, effectively controlling the flow rate of the reaction solution when flowing from the top reaction cavity to the bottom reaction cavity; S4: There is a movable component at the top of the reaction chamber. Carbon dioxide will be generated when the reaction liquid reacts with the enzyme. The reaction chamber is a closed box. When the generated gas pushes the piston to move upward, the pointer connected to the side of the piston presses the pressing rod for real-time monitoring. When the rate is too low, the high-concentration reaction liquid in the high-concentration liquid tank on the side is added to accelerate the catalytic reaction of the enzyme. When the rate is too high, the rotation speed of the rotating blade is slowed down.

[0012] Compared with the prior art, the present invention has the following beneficial effects: 1. In the ATP immobilized enzyme catalytic synthesis device and synthesis process, the reaction chamber is an inclined box with a slope, which avoids the reduction of enzyme activity due to the long-term contact between the reaction liquid and the enzyme after entering the box, thus preventing the improvement of catalytic efficiency. When the reaction liquid enters the interior of the reaction chamber through the second interface, there is a rotating column on the inner wall of the reaction chamber. The rotating column drives the rotating blades arranged in a circular array to rotate. There are multiple placement plates on the outer wall of the rotating column, and circular holes are opened on the surface of the placement plates. The porous carrier makes it easier for the reaction liquid to diffuse to the active sites of the enzyme, improving the catalytic efficiency. On the inner wall of the reaction chamber, there are a first sliding plate and a second sliding plate. When the reaction liquid flows from the top reaction chamber to the bottom reaction chamber, the partition effectively avoids the splashing of the reaction liquid caused by the inertial force when the reaction liquid flows from a high place to a low place.

[0013] 2. In the ATP immobilized enzyme catalytic synthesis device and synthesis process, when the rotating blade rotates and presses the button, since the initial positions of the two sliding plates are at the left and right ends of the inner wall of the reaction chamber, the first sliding plate and the second sliding plate slide in opposite directions. The rotation speed of the rotating blade is equal to the moving speed of the first sliding plate and the second sliding plate. The setting of the first sliding plate and the second sliding plate effectively controls the flow rate of the falling reaction liquid. When the immobilized enzyme reacts too fast or too slow, it is effectively controlled, improving the accuracy of the equipment, so that the enzyme will not have low catalytic efficiency due to too fast flow rate of the reaction liquid, nor will it have its activity reduced due to too slow flow rate of the reaction liquid and long-term contact with the immobilized enzyme, resulting in a decrease in work efficiency.

[0014] 3. In the ATP immobilized enzyme catalytic synthesis device and synthesis process, there is also a monitoring component on the outer wall of the connecting rod. When the piston drives the connecting rod to move, when the pressing rod detects that the moving speed of the pointer is relatively fast, the speed measurement box adjusts the driving motor to reduce the rotation rate of the rotating blade and slow down the flow rate of the reaction liquid in the reaction chamber, so that the reaction liquid can fully carry out catalytic reaction with the immobilized enzyme. When it is detected that the moving speed of the pointer is relatively slow, the high-concentration liquid tank on the side wall is opened, so that the high-concentration reaction liquid in the high-concentration liquid tank enters the reaction chamber for full combination to accelerate the reaction rate. Description of the Drawings

[0015] Figure 1It is a schematic structural diagram of an ATP immobilized enzyme catalytic synthesis device and a synthesis process; Figure 2 It is the second schematic structural diagram of an ATP immobilized enzyme catalytic synthesis device and a synthesis process; Figure 3 It is a schematic structural diagram of a reaction tank of an ATP immobilized enzyme catalytic synthesis device and a synthesis process; Figure 4 It is a schematic sectional view of a reaction tank of an ATP immobilized enzyme catalytic synthesis device and a synthesis process; Figure 5 It is a schematic structural diagram of a placement component of an ATP immobilized enzyme catalytic synthesis device and a synthesis process; Figure 6 It is the second schematic sectional view of a placement component of an ATP immobilized enzyme catalytic synthesis device and a synthesis process; Figure 7 It is a schematic sectional view of a reaction tank of an ATP immobilized enzyme catalytic synthesis device and a synthesis process; Figure 8 It is a schematic structural diagram of a movable component of an ATP immobilized enzyme catalytic synthesis device and a synthesis process.

[0016] In the figure: 1, catalytic tank; 2, placement groove; 3, outlet; 4, reaction liquid placement tank; 5, high-concentration liquid tank; 6, first connecting pipe; 7, first interface; 8, second interface; 9, second connecting pipe; 10, driving motor; 11, connecting block; 12, monitoring component; 121, speed measurement box; 122, pressing rod; 13, movable component; 131, movable cavity; 132, piston; 133, connecting rod; 134, pointer; 14, reaction tank; 15, placement component; 151, fixing block; 152, rotating column; 153, rotating blade; 154, connecting long rod; 155, placement plate; 156, round hole; 16, button; 17, first sliding groove; 18, first sliding plate; 19, T-shaped guide plate; 20, second sliding groove; 21, second sliding plate. Detailed implementation manners

[0017] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0018] Please refer to Figure 1-8, an ATP immobilized enzyme catalytic synthesis device, including a catalytic box 1. The outer wall of the catalytic box 1 is fixedly connected with a reaction liquid placement box 4. On one side of the outer wall of the catalytic box 1 away from the reaction liquid placement box 4, a second connecting pipe 9 is fixedly connected. The outer wall of the second connecting pipe 9 is fixedly connected with a second interface 8. On one side of the outer wall of the catalytic box 1 away from the reaction liquid placement box 4, a high-concentration liquid box 5 is provided. The outer wall of the high-concentration liquid box 5 is fixedly connected with a first connecting pipe 6. The outer wall of the first connecting pipe 6 is fixedly connected with a first interface 7. Inside the catalytic box 1, there is a reaction box 14. Inside the reaction box 14, there is a placement assembly 15. The placement assembly 15 includes a fixed block 151 on the inner wall of the reaction box 14. A driving motor 10 is provided on the outer wall of the fixed block 151. At the other end of the outer wall of the fixed block 151, there is a rotating column 152. A rotating blade 153 is provided on the outer wall of the rotating column 152. A connecting long rod 154 is provided on the outer wall of the rotating column 152. A plurality of placement disks 155 are provided on the outer wall of the connecting long rod 154. The plurality of placement disks 155 are linearly arrayed on the outer wall of the connecting long rod 154. Circular holes 156 are opened on the outer walls of the plurality of placement disks 155. A button 16 is provided on the inner wall of the reaction box 14. The button 16 and the rotating blade 153 are on the same horizontal line. A first sliding groove 17 is opened on the inner wall of the reaction box 14. A first sliding plate 18 is slidably connected to the outer wall of the first sliding groove 17. A second sliding groove 20 is opened on the inner wall of the reaction box 14 near the first sliding groove 17. A second sliding plate 21 is slidably connected to the outer wall of the second sliding groove 20.

[0019] It should be noted that the reaction box 14 is an inclined box with a slope to prevent the reaction liquid from reducing the enzyme activity due to long-term contact with the enzyme after entering the box, thus unable to improve the catalytic efficiency. When the reaction liquid enters the interior of the reaction box 14 through the second interface 8, there is a rotating column 152 on the inner wall of the reaction box 14. The rotating column 152 drives the rotating blades 153 arranged in a circular array to rotate. A plurality of placement disks 155 are provided on the outer wall of the rotating column 152. Circular holes 156 are opened on the surfaces of the placement disks 155. The porous carrier makes it easier for the reaction liquid to diffuse to the active sites of the enzyme, improving the catalytic efficiency. First sliding plate 18 and second sliding plate 21 are provided on the inner wall of the reaction box 14. When the reaction liquid flows from the top reaction chamber to the bottom reaction chamber, the partition effectively prevents the reaction liquid from splashing due to inertial force when flowing from a high place to a low place.

[0020] Please refer to Figure 7 : The first sliding plate 18 and the second sliding plate 21 are respectively located at the left and right ends of the inner wall of the reaction box 14, and slide in opposite directions between the first sliding plate 18 and the second sliding plate 21.

[0021] It should be noted that when the rotating blade 153 rotates and presses the button 16, since the initial positions of the two sliding plates are at the left and right ends of the inner wall of the reaction tank 14, the first sliding plate 18 and the second sliding plate 21 slide in opposite directions. The rotation speed of the rotating blade 153 is equal to the moving speed of the first sliding plate 18 and the second sliding plate 21. The settings of the first sliding plate 18 and the second sliding plate 21 effectively control the flow rate of the falling reaction liquid. When the immobilized enzyme reacts too fast or too slow, it is effectively controlled, improving the accuracy of the equipment. This ensures that the enzyme will not have low catalytic efficiency due to too fast a flow rate of the reaction liquid during catalysis, nor will it have its activity reduced due to long-term contact with the immobilized enzyme due to too slow a flow rate of the reaction liquid, resulting in a decrease in work efficiency.

[0022] Please refer to Figure 8 : An activity component 13 is provided on the outer wall of the reaction tank 14. The activity component 13 includes an activity cavity 131 on the outer wall of the reaction tank 14. A piston 132 is provided on the inner wall of the activity cavity 131. A connecting rod 133 is fixedly connected to the outer wall of the piston 132, and a pointer 134 is provided on the outer wall of the connecting rod 133.

[0023] It should be noted that when the immobilized enzyme catalyzes, carbon dioxide is generated. Since the reaction tank 14 is a closed box, when the enzyme reaction is too fast, the piston 132 in the activity cavity 131 moves quickly upward. Since the connecting rod 133 is fixedly connected to the outer wall of the piston 132 and the pointer 134 is provided on the outer wall of the connecting rod 133, when the pointer moves, it drives the monitoring component 12 connected to the outer wall to monitor the catalytic reaction rate in the inner cavity. When the enzyme reaction is too slow, the piston 132 moves slowly in the inner cavity.

[0024] Please refer to Figure 8 : A monitoring component 12 is provided on the outer wall of the connecting rod 133. The monitoring component 12 includes a speed measurement box 121 on the outer wall of the connecting rod 133. A pressing rod 122 is provided on the outer wall of the speed measurement box 121, and a connecting block 11 is fixedly connected to the outer wall of the speed measurement box 121.

[0025] It should be noted that a monitoring component 12 is provided on the outer wall of the connecting rod 133. When the piston drives the connecting rod 133 to move, when the pressing rod 122 detects that the pointer 134 moves at a relatively fast speed, the speed measurement box 121 adjusts the driving motor 10 to reduce the rotation speed of the rotating blade 153, slowing down the flow rate of the reaction liquid in the reaction tank, so that the reaction liquid can fully undergo a catalytic reaction with the immobilized enzyme. When it detects that the pointer 134 moves at a relatively slow speed, the high-concentration liquid tank 5 on the side wall is opened, so that the high-concentration reaction liquid in the high-concentration liquid tank 5 enters the reaction tank for full combination to accelerate the reaction rate.

[0026] Please refer to Figure 3 : The driving motor 10 is electrically connected to the monitoring component 12.

[0027] It should be noted that since the drive motor 10 and the monitoring component 12 are electrically connected, the device effectively controls the reaction rate of the enzyme, preventing the catalytic efficiency from being low due to the too-fast flow rate of the reaction solution during enzyme catalysis, and also preventing the enzyme from being in contact with the immobilized enzyme for a long time due to the too-slow flow rate of the reaction solution, which reduces the enzyme activity and leads to a decrease in work efficiency.

[0028] Please refer to Figure 8 : The outer wall of the pointer 134 is in contact with the pressing rod 122.

[0029] It should be noted that when the pointer 134 moves, the pressing rod 122 is pressed to effectively monitor the moving speed of the piston 132, and effectively monitor the reaction rate in the reaction chamber.

[0030] Please refer to Figure 5 : The rotation speed of the rotating blade 153 is different from the rotation speed of the connecting long rod 154.

[0031] It should be noted that the rotation speed of the connecting long rod 154 always remains uniform. When the piston drives the pointer 134 to move too fast, since the monitoring component 12 and the drive motor 10 are electrically connected, the rotation speed of the rotating blade 153 is effectively reduced. When the rotating blade 153 with the reduced speed rotates, the bottom button 16 is pressed to make the sliding plate slide, reducing the flow rate of the reaction solution in the reaction chamber.

[0032] Please refer to Figure 1 : The outer wall of the catalytic box 1 is provided with an outlet 3, the top of the catalytic box 1 is provided with a placement groove 2, and the inner wall of the reaction box 14 is fixedly connected with a T-shaped guide plate 19.

[0033] It should be noted that when the reaction solution that has reacted with the enzyme flows out from the outlet 3, the T-shaped guide plate 19 fixedly connected to the inner wall of the reaction box 14 reduces the splashing caused by inertia when the reaction solution flowing down through the sliding plate flows towards the lower place.

[0034] An ATP immobilized enzyme catalytic synthesis process includes the following processes: S1: The reaction solution enters the inner wall of the reaction box 14 through the second connecting pipe 9. The reaction box 14 is set as an inclined plate with a slope. When the liquid enters the reaction box 14, it flows from a high place to a low place and then flows out from the outlet 3 at the bottom of the reaction box 14; S2: When the reaction solution enters the reaction box 14, since the inner wall is provided with a rotating blade 153, the rotating blade 153 pushes the liquid to flow, preventing sedimentation and accumulation, and ensuring that the placement plate 155 provided on the outer wall of the connecting long rod 154 is in full contact with the reaction solution when rotating; S3: A button 16 is provided at the bottom of the rotating blade 153. When the rotating blade 153 rotates, the blade presses the button 16, causing the first sliding plate 18 and the second sliding plate 21 to slide in the sliding groove, effectively controlling the flow rate of the reaction liquid when it flows from the top reaction chamber into the bottom reaction chamber; S4: An active component 13 is provided at the top of the reaction tank 14. Carbon dioxide will be generated when the reaction liquid reacts with the enzyme. The reaction tank 14 is a closed box. When the generated gas pushes the piston 132 upward, the pointer 134 connected to the side of the piston 132 presses the pressing rod 122 for real-time monitoring. When the rate is too low, the high-concentration reaction liquid in the high-concentration liquid tank 5 on the side is added to accelerate the catalytic reaction of the enzyme. When the rate is too high, the rotation speed of the rotating blade 153 is slowed down.

[0035] Working principle: When the device needs to be used, after the reaction liquid enters the interior of the reaction tank 14 through the second interface 8, a rotating column 152 is provided on the inner wall of the reaction tank 14. The rotating column 152 drives the rotating blades 153 arranged in a circular array to rotate. A plurality of placement disks 155 are provided on the outer wall of the rotating column 152, and round holes 156 are opened on the surface of the placement disks 155. The porous carrier makes it easier for the reaction liquid to diffuse to the active sites of the enzyme, improving the catalytic efficiency. A first sliding plate 18 and a second sliding plate 21 are provided on the inner wall of the reaction tank 14. When the reaction liquid flows from the top reaction chamber into the bottom reaction chamber, the partition effectively prevents the reaction liquid from splashing due to inertia force when flowing from a high place to a low place; When the rotating blade 153 rotates and presses the button 16, since the initial positions of the two sliding plates are at the left and right ends of the inner wall of the reaction tank 14, the first sliding plate 18 and the second sliding plate 21 slide in opposite directions. The rotation speed of the rotating blade 153 is equal to the moving speed of the first sliding plate 18 and the second sliding plate 21. The settings of the first sliding plate 18 and the second sliding plate 21 effectively control the flow rate of the falling reaction liquid. Since the reaction tank 14 is a closed box, a monitoring component 12 is provided on the outer wall of the connecting rod 133. When the piston drives the connecting rod 133 to move, since the rotation speed of the connecting long rod 154 always remains uniform, when the pressing rod 122 detects that the moving speed of the pointer 134 is fast, the speed measurement box 121 adjusts the driving motor 10 to reduce the rotation speed of the rotating blade 153 and slow down the flow speed of the reaction liquid in the reaction tank, so that the reaction liquid can fully react with the immobilized enzyme. When it is detected that the moving speed of the pointer 134 is slow, the high-concentration liquid tank 5 on the side wall is opened to allow the high-concentration reaction liquid in the high-concentration liquid tank 5 to enter the reaction tank for full combination to accelerate the reaction rate.

[0036] Although embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An ATP immobilized enzyme catalytic synthesis device, comprising a catalyst box (1), characterized in that: The outer wall of the catalyst box (1) is fixedly connected to a reaction liquid placement box (4); the outer wall of the catalyst box (1) is fixedly connected to a second connecting pipe (9) on a side away from the reaction liquid placement box (4); the outer wall of the second connecting pipe (9) is fixedly connected to a second interface (8); the outer wall of the catalyst box (1) is provided with a high-concentration liquid box (5) on a side away from the reaction liquid placement box (4); the outer wall of the high-concentration liquid box (5) is fixedly connected to a first connecting pipe (6); the outer wall of the first connecting pipe (6) is fixedly connected to a first interface (7); the inner wall of the catalyst box (1) is provided with a reaction box (14); the inner wall of the reaction box (14) is provided with a placement component (15); The placement component (15) comprises a fixed block (151) located on the inner wall of the reaction box (14); a driving motor (10) is provided on the outer wall of the fixed block (151); a rotating column (152) is provided on the other end of the outer wall of the fixed block (151); a rotating blade (153) is provided on the outer wall of the rotating column (152); a connecting long rod (154) is provided on the outer wall of the connecting long rod (154); a plurality of placement plates (155) are provided on the outer wall of the connecting long rod (154); the plurality of placement plates (155) are distributed on the outer wall of the connecting long rod (154) in a linear array; and circular holes (156) are provided on the outer walls of the plurality of placement plates (155); The inner wall of the reaction box (14) is provided with a button (16), and the button (16) and the rotating blade (153) are located on the same horizontal line. The inner wall of the reaction box (14) is provided with a first sliding groove (17), and the outer wall of the first sliding groove (17) is slidably connected to a first sliding plate (18). The inner wall of the reaction box (14) is provided with a second sliding groove (20) on a side close to the first sliding groove (17), and the outer wall of the second sliding groove (20) is slidably connected to a second sliding plate (21).

2. The ATP immobilized enzyme catalytic synthesis device according to claim 1, characterized in that: The first sliding plate (18) and the second sliding plate (21) are respectively located at the left and right ends of the inner wall of the reaction box (14), and the first sliding plate (18) and the second sliding plate (21) slide in opposite directions.

3. The ATP immobilized enzyme catalytic synthesis device according to claim 1, characterized in that: The outer wall of the reaction box (14) is provided with a movable component (13), the movable component (13) comprising an movable cavity (131) located on the outer wall of the reaction box (14), the inner wall of the movable cavity (131) is provided with a piston (132), the outer wall of the piston (132) is fixedly connected to a connecting rod (133), and the outer wall of the connecting rod (133) is provided with a pointer (134).

4. The ATP immobilized enzyme catalytic synthesis device according to claim 3, characterized in that: The outer wall of the connecting rod (133) is provided with a monitoring assembly (12), the monitoring assembly (12) comprising a speed measuring box (121) located on the outer wall of the connecting rod (133), the outer wall of the speed measuring box (121) is provided with a pressing rod (122), and the outer wall of the speed measuring box (121) is fixedly connected to a connecting block (11).

5. The ATP immobilized enzyme catalytic synthesis device according to claim 4, characterized in that: The driving motor (10) is electrically connected to the monitoring component (12).

6. The ATP immobilized enzyme catalytic synthesis device according to claim 3, characterized in that: The outer wall of the pointer (134) fits the outer wall of the pressing rod (122).

7. The ATP immobilized enzyme catalytic synthesis device according to claim 1, characterized in that: The rotation speed of the rotating blade (153) is different from the rotation speed of the connecting long rod (154).

8. The ATP immobilized enzyme catalytic synthesis device according to claim 1, characterized in that: An outlet (3) is provided on the outer wall of the catalyst box (1), a placement groove (2) is provided on the top of the catalyst box (1), and a T-shaped guide plate (19) is fixedly connected to the inner wall of the reaction box (14).

9. An ATP immobilized enzyme catalytic synthesis process, applied to an ATP immobilized enzyme catalytic synthesis device as claimed in any one of claims 1 to 8, characterized in that: Including the following processes: S1: The reaction liquid enters the inner wall of the reaction box (14) through the second connecting pipe (9). The reaction box (14) is configured as an inclined plate with a slope. When the liquid enters the reaction box (14), it flows from a high position to a low position to the bottom of the reaction box (14) and then flows out from the outlet (3); S2: When the reaction liquid enters the reaction box (14), the rotating blades (153) provided on the inner wall push the liquid to flow, preventing it from settling and accumulating on the bottom, thereby ensuring that the placement plate (155) provided on the outer wall of the connecting long rod (154) is in full contact with the reaction liquid during rotation; S3: A button (16) is provided at the bottom of the rotating blade (153). When the rotating blade (153) rotates, the blade presses the button (16), so that the first sliding plate (18) and the second sliding plate (21) slide in the sliding groove, thereby effectively controlling the flow rate of the reaction liquid when it flows from the top reaction chamber into the bottom reaction chamber; S4: A movable component (13) is provided on the top of the reaction box (14). Carbon dioxide is generated when the reaction liquid reacts with the enzyme. The reaction box (14) is a closed box. When the generated gas pushes the piston (132) to move upward, the pointer (134) connected to the side of the piston (132) presses the pressing rod (122) for real-time monitoring. When the rate is too low, the high-concentration reaction liquid in the high-concentration liquid box (5) on the side is added to accelerate the catalytic reaction of the enzyme. When the rate is too high, the rotation speed of the rotating blade (153) is slowed down.

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