Composition for tonifying qi and blood, aiding sleep and improving memory, and preparation method and application thereof

Abstract

The application provides a composition for tonifying qi and blood, improving sleep and memory and a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine.The composition comprises, in terms of weight parts, 5-50 parts of astragalus root, 5-30 parts of angelica root, 5-25 parts of hawthorn, 1-15 parts of dried tangerine or orange peel, 5-40 parts of mulberry, 5-25 parts of malt, 1-15 parts of dendrobium and 1-25 parts of American ginseng.The dendrobium is first decocted, and then subjected to enzymolysis by using a compound enzyme of cellulase and pectinase to obtain a dendrobium extract;the astragalus root, the angelica root, the malt and the American ginseng are soaked in water, subjected to ultrasonic treatment and decocted to obtain filter residue 1 and filter liquid 1;then the filter residue is mixed with the hawthorn, the dried tangerine or orange peel and the mulberry, and further decocted in water to obtain filter liquid 2;the dendrobium extract and all the filter liquids are combined to obtain the composition.The composition has the effects of tonifying qi and blood, improving immunity, improving insomnia and improving memory due to the mutual cooperation of the raw material components.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of traditional Chinese medicine, and particularly relates to a composition for tonifying qi and blood, assisting sleep and improving memory, a preparation method and application thereof. BACKGROUND

[0002] Western medicine usually uses sedative hypnotics, anxiolytics, antidepressants or hormone replacement therapy for insomnia. During the medication process, the following adverse reactions are prone to occur: memory loss, decreased attention, fatigue, and deficiency of qi and blood. Long-term use can also cause tolerance and dependence. Traditional Chinese medicine refers to insomnia as "not sleeping", and the main disease location of sleep is in the heart and brain. Insomnia is caused by heart and mind deficiency or disturbance, and the mind is not stable. It is also caused by kidney essence deficiency, brain loss of nourishment, and the mind not being held. Overexertion of the mind and excessive consumption of yin blood can lead to deficiency of qi and blood, which cannot nourish the heart and mind, and the brain loses its nourishment, resulting in insomnia. Deficiency of qi and blood also makes it difficult to promote blood circulation, causing blood stasis, accelerating the aging process, and causing memory impairment and reduced immunity.

[0003] At present, there are many traditional Chinese medicine compositions for tonifying qi and blood and improving insomnia. Different compositions are used for different etiology and pathogenesis and different syndromes. Chinese invention patent CN103520368A discloses a traditional Chinese medicine health care preparation for tonifying qi and blood and enhancing immunity, which is prepared from the following raw medicinal materials by weight: 5-25 parts of donkey-hide gelatin, 15-35 parts of radix codonopsis, 10-30 parts of angelica sinensis, 10-30 parts of astragalus root, 5-25 parts of medlar, 1-5 parts of deer horn, and 1-5 parts of blood red ferrite. The preparation of the invention has certain effects on spleen and stomach weakness, fatigue, low immunity, waist pain, abdominal distension, insomnia, forgetfulness, irregular menstruation, menopausal syndrome, and other symptoms caused by deficiency of qi and blood.

[0004] Chinese invention patent CN116350725A discloses a traditional Chinese medicine composition for treating insomnia of heart and spleen deficiency type, which is prepared from the following raw medicinal materials by weight: 20-30 parts of astragalus membranaceus, 15-25 parts of atractylodes, 20-30 parts of angelica sinensis, 15-25 parts of pericarpium citri reticulatae, 30-40 parts of hawthorn, 20-30 parts of malt, 15-25 parts of raphanus sativus, 10-20 parts of rhizoma cyperi, 20-30 parts of semen ziziphi spinosae, 10-20 parts of semen ziziphi spinosae, 20-30 parts of poria cocos, 10-20 parts of licorice, 10-20 parts of poria cocos, 15-25 parts of radix rehmanniae preparatae, 10-20 parts of chuanxiong, 15-25 parts of polygala, 8-12 parts of fritillaria, 15-20 parts of aconitum, 10-20 parts of bupleurum, and 10-20 parts of anemarrhena. The traditional Chinese medicine composition of the invention can tonify the heart and spleen, harmonize the stomach and stagnation, calm the heart and mind, nourish the blood and calm the mind, resolve phlegm and dampness, and soothe the liver and relieve restlessness, and has good effects on treating insomnia of heart and spleen deficiency type. However, the traditional Chinese medicine composition does not have the effect of improving memory.

[0005] In addition, Chinese invention patent CN116036224A discloses a traditional Chinese medicine composition for improving sleep and learning and memory ability as well as a preparation method and application thereof. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: Cordyceps sinensis 0.1-0.5 parts, Radix Paeoniae Alba 15-20 parts, Ramulus Cinnamomi 10-15 parts, Fructus Jujubae 28-32 parts, Rhizoma Zingiberis 3-7 parts, Radix Notoginseng 10-15 parts, Radix Rehmanniae 5-10 parts, Rhizoma Polygonati 15-20 parts, Radix Angelicae Sinensis 10-15 parts, Radix Astragali 15-20 parts, and gelatin 3-7 parts. The traditional Chinese medicine composition of the invention can tonify qi and blood, produce fluid and transform essence to calm the mind and pacify the spirit, and is mainly suitable for people with insufficient essence and blood, poor sleep quality, forgetfulness or decreased learning and memory ability.

[0006] Although there are many traditional Chinese medicine compositions for tonifying qi and blood and improving insomnia at present, most of the compositions have many traditional Chinese medicine ingredients, the composition is complex, and most of them do not reflect the synergistic effect, and the effect of improving memory, tonifying qi and blood, and helping sleep is weak. Therefore, it is necessary to further study the selection and ratio of the components of the traditional Chinese medicine composition, and to provide a traditional Chinese medicine composition with a simple composition, mutual interaction of components, synergistic effect, and the ability to significantly improve insomnia, improve memory, and tonify qi and blood. SUMMARY

[0007] The present application provides a composition for tonifying qi and blood, helping sleep, and improving memory as well as a preparation method and application thereof, which uses Astragalus, Angelica, Hawthorn, Pericarpium Citri Reticulatae, Morus alba, malt, Dendrobium and Panax quinquefolium in combination to achieve the effects of tonifying qi and blood, improving immunity, improving insomnia, and improving memory.

[0008] To achieve the above-mentioned object, the technical scheme adopted by the present application is as follows:

[0009] Firstly, the present application provides a composition for tonifying qi and blood, helping sleep, and improving memory, which comprises the following raw material components in parts by weight: 5-50 parts of Astragalus, 5-30 parts of Angelica, 5-25 parts of Hawthorn, 1-15 parts of Pericarpium Citri Reticulatae, 5-40 parts of Morus alba, 5-25 parts of malt, 1-15 parts of Dendrobium, and 1-25 parts of Panax quinquefolium.

[0010] Preferably, the composition comprises the following raw material components in parts by weight: 12-20 parts of Astragalus, 8-15 parts of Angelica, 12-20 parts of Hawthorn, 8-12 parts of Pericarpium Citri Reticulatae, 13-16 parts of Morus alba, 12-20 parts of malt, 8-12 parts of Dendrobium, and 13-20 parts of Panax quinquefolium.

[0011] Further preferably, the composition comprises the following raw material components in parts by weight: 16 parts of Astragalus, 12 parts of Angelica, 15 parts of Hawthorn, 10 parts of Pericarpium Citri Reticulatae, 14 parts of Morus alba, 15 parts of malt, 10 parts of Dendrobium, and 16 parts of Panax quinquefolium.

[0012] Then, the present application provides a preparation method of the above composition, comprising steps of:

[0013] (1) preparing the dendrobium extract: decocting dendrobium with water to obtain a decoction mixture; adding a compound enzyme to the decoction mixture after cooling to perform enzymolysis, to obtain the dendrobium extract; the compound enzyme is cellulase and pectinase;

[0014] (2) soaking radix angelica sinensis, radix angelicae sinensis, malt and western corn root with water, ultrasonic treatment, decocting, filtering to obtain filtrate 1 and residue 1;

[0015] (3) mixing residue 1 with hawthorn, dried tangerine or orange peel and mulberry, and continuing to decoct with water, filtering to obtain filtrate 2;

[0016] (4) combining filtrate 1 and filtrate 2, concentrating and drying to obtain a mixture; mixing the mixture and the dendrobium extract to obtain the composition.

[0017] Preferably, in step (1), the solid-liquid ratio of the decoction is 1:8-20 g / mL, and the decoction is performed for 1-3 h.

[0018] Further preferably, in step (1), the solid-liquid ratio of the decoction is 1:15 g / mL, and the decoction is performed for 2 h.

[0019] Preferably, in step (1), the cooling temperature is 38-45℃.

[0020] Further preferably, in step (1), the cooling temperature is 42℃.

[0021] Preferably, in step (1), the mass ratio of cellulase and pectinase is 1:1.2-5.

[0022] Further preferably, in step (1), the mass ratio of cellulase and pectinase is 1:3.

[0023] Preferably, in step (1), the addition amount of the compound enzyme is 1-3 mg compound enzyme / 50-300 mg dendrobium.

[0024] Further preferably, in step (1), the addition amount of the compound enzyme is 2 mg compound enzyme / 200 mg dendrobium.

[0025] Preferably, in step (1), the enzymolysis temperature is 38-45℃, and the enzymolysis time is 18-48 h.

[0026] Further preferably, in step (1), the enzymolysis temperature is 42℃, and the enzymolysis time is 36 h.

[0027] Preferably, in step (2), the solid-liquid ratio of the water soaking is 1:8-20 g / mL.

[0028] Further preferably, in step (2), the solid-liquid ratio of the water-soaking is 1:15 g / mL.

[0029] Preferably, in step (2), the time of the ultrasonic treatment is 0.5-2 h.

[0030] Further preferably, in step (2), the time of the ultrasonic treatment is 1 h.

[0031] Preferably, in step (2), the time of the decoction is 0.5-2 h.

[0032] Further preferably, in step (2), the time of the decoction is 1 h.

[0033] Preferably, in step (3), the solid-liquid ratio of the decoction is 1:6-15 g / mL, and the decoction is performed for 1-3 h.

[0034] Preferably, in step (3), the solid-liquid ratio of the decoction is 1:10 g / mL, and the decoction is performed for 2 h.

[0035] In addition, the present application provides use of the above-mentioned composition in the preparation of a medicament having the effects of tonifying qi and blood, aiding sleep, and improving memory.

[0036] Finally, the present application provides a medicament having the effects of tonifying qi and blood, aiding sleep, and improving memory, comprising the component: the above-mentioned composition.

[0037] Preferably, the medicament further comprises the component: a pharmaceutical carrier.

[0038] The pharmaceutical carrier used can be a solid, liquid, or gas. Examples of solid carriers include lactose, kaolin, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, and stearic acid. Examples of liquid carriers include syrup, peanut oil, olive oil, and water. Examples of gaseous carriers include carbon dioxide and nitrogen.

[0039] In preparing the compositions in oral dosage forms, any convenient pharmaceutical media can be employed. For example, water, ethanol, oils, alcohols, flavoring agents, preservatives, coloring agents and the like can be used to form oral liquid preparations such as suspensions, solutions, and elixirs; while carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, emulsifiers, lubricants, binders, and disintegrating agents can be used to form oral solid preparations such as powders, capsules, and tablets. Because of their ease of administration, tablets and capsules are preferred oral dosage units. Optionally, tablets can be coated by standard aqueous or nonaqueous techniques.

[0040] Tablets containing the herbal composition or extract of the present application can be prepared by compression or molding, optionally, with one or more accessory ingredients or adjuvants. Compressed tablets can be prepared by compressing in a free-flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, surface active or dispersing agent. Molded tablets can be made by molding in a suitable machine a mixture of the powdered compound moistened with a liquid diluent. Each tablet preferably contains from about 0.05 mg to about 5 g of the active ingredient. Each cachet or capsule preferably contains from about 0.05 mg to about 5 g of the active ingredient. For instance, a formulation intended to be administered to the human subject orally can contain from about 0.5 mg to about 5 g of the active drug substance compounded with an appropriate and convenient amount of a carrier material which can take a form of tablets, pills, capsules, cachets, etc. Unit dosages will generally contain between about 1 mg and about 2 g, often about 25 mg, 50 mg, 100 mg, 200 mg, 300 mg, 400 mg, 500 mg, 600 mg, 800 mg or 1000 mg of the active ingredient.

[0041] Pharmaceutical compositions of the present application suitable for parenteral administration can be formulated as solutions or suspensions of the active compounds in either aqueous or non-aqueous liquids, such as vegetable oils, propylene glycol, polyethylene glycol, or injectable organic esters, such as ethyl oleate. Proper fluidity can be maintained, for example, by the use of co-solvents, such as propylene glycol, in the case of injectable solutions. These compositions can also contain suitable surface active or dispersing agents, such as hydroxypropylcellulose.

[0042] The pharmaceuticals of the present application can be in a form suitable for local administration, e.g., as an aerosol, cream, ointment, lotion, powder or the like. Also, the composition can be in a form suitable for use in a transdermal delivery system. These formulations can be prepared conventionally by mixing the herbal composition of the present application with conventional carriers, diluents or excipients. For example, creams or ointments can be formulated by mixing the active ingredient with a suitable mixture of a hydrophilic material and water, and about 5% to about 10% by weight of the compound.

[0043] The pharmaceuticals of the present application can be in a form suitable for rectal administration wherein the carrier is a solid. The compound mixture is preferably formed into unit dose suppositories. Suitable carriers include cocoa butter and other materials often used in making suppositories. The suppositories can be prepared by mixing the ingredients, melting a combination of the compound in a soft or fluid vehicle, and then cooling the molten mixture to form a solid mass.

[0044] In addition to the carrier ingredients, the pharmaceutical formulations described above can include, as appropriate, one or more of the following: diluents, buffers, flavoring agents, binders, surface active agents, thickeners, lubricants, preservatives (including antioxidants), colorants, and the like. Additional excipients can be added, such as lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like, coloring agents and flavoring agents. The formulations are provided in a form suitable to the intended subject, e.g., a human subject. The components of the formulations are mixed in a manner appropriate for the formulation. The components of the formulations can be provided in a powder or liquid concentrate form.

[0045] Preferably, the dosage form of the medicine is not limited to pills, capsules, granules, oral liquids, powders, tablets, lozenges, pastes and the like administration forms, and suitable pharmaceutical carriers in the art can be selected for different dosage forms.

[0046] Solution:

[0047] 1. Core gas-blood dual-supplement framework: Huangqi is used as the monarch drug to fully exert its effects of tonifying qi and ascending yang, stabilizing the exterior and stopping sweating, and promoting water and relieving swelling, thereby promoting the generation and operation of qi and blood in the whole body; Danggui is used as the minister drug to tonify and activate blood, regulate menstruation and relieve pain, and follow the principle of "Danggui Buxue Decoction" of mutual generation of qi and blood, thereby strengthening the effects of tonifying qi and generating blood through collocation and proportioning, and ensuring sufficient and smooth flow of qi and blood.

[0048] 2. Spleen and stomach transportation and transformation coordination system: Chenpi can regulate qi, invigorate the spleen, dry dampness and resolve phlegm, thereby resolving the greasy taste of tonifying drugs, and unblocking the transportation and transformation of the spleen and stomach, which is a minister drug and is helpful to the absorption of other medicinal materials; Shanzha can digest food and invigorate the stomach, and disperse blood stasis, thereby assisting the digestion of the spleen and stomach; Maiya can disperse qi and digest food, invigorate the spleen and open the stomach, and form a coordination system with Chenpi and Shanzha to promote the absorption and transformation of nutrients by the spleen and stomach, and lay a solid foundation for the generation of qi and blood.

[0049] 3. Yin-nourishing and heat-clearing balance mechanism: Sangshen can nourish yin and blood, generate fluid and moisten dryness, and nourish the yin of liver and kidney, thereby providing protection for the generation of yin and blood; Shihu can benefit the stomach, generate fluid and nourish yin and clear heat, and nourish the yin of the stomach to assist the transportation and transformation of the spleen and stomach, and cooperate with Sangshen to regulate liver and kidney yin deficiency; Xiyangshen can tonify qi and nourish yin, clear heat and generate fluid, and assist Huangqi in tonifying qi, thereby balancing the warm and dry nature of Huangqi and Danggui, and supplementing yin for the body, so that the whole prescription maintains the balance of yin and yang when tonifying qi and blood, and avoids fire.

[0050] 4. Nerve regulation and improvement of brain function: Traditional Chinese medicine believes that the heart governs consciousness, and the basis for the normal heart governing consciousness is that qi and blood are full and yin and yang are in harmony. The composition of the present application can tonify qi and blood, nourish the liver and kidney, and nourish the heart to improve sleep and memory.

[0051] In the present application, Huangqi is the dried root of Astragalus mongholicus or Astragalus membranaceus of the Leguminosae family, and has the effects of tonifying heart and spleen and tonifying qi and stabilizing the exterior.

[0052] In the present application, Danggui is sweet and pungent, and warm, and belongs to the liver, heart and spleen meridians, and has the effects of tonifying blood, activating blood, regulating menstruation and relieving pain, moistening the intestines and defecation, and tonifying heart and spleen.

[0053] In the present application, Shanzha belongs to the spleen, stomach and liver meridians, and has the effects of harmonizing the stomach and resolving stagnation, digesting food and resolving accumulation, and dispersing blood stasis.

[0054] In the present application, Chenpi belongs to the lung and spleen meridians, and has the effects of regulating qi, invigorating the spleen and opening the stomach.

[0055] In the present application, the mulberry is the mature fruit of the deciduous tree Morus alba Linn; it is sweet, sour and cold in nature, and belongs to the heart, liver and kidney channels; it can be used to treat yin blood deficiency, dry mouth and thirst, constipation, and has the effects of lowering lipid and blood pressure, and invigorating the spleen and nourishing the stomach.

[0056] In the present application, the malt is sweet and flat in nature, and belongs to the spleen and stomach channels; it has the effects of promoting digestion, invigorating the spleen and stomach, diuresis, and calming the nerves.

[0057] In the present application, the dendrobium is sweet in taste and slightly cold in nature, and belongs to the stomach and kidney channels; it is used for heat disease, dry mouth and thirst, stomach yin deficiency, loss of appetite and vomiting, post-illness heat, yin deficiency and heat, bone heat, dark eyes, and soft muscles and bones; it has the effects of improving sleep and calming the nerves, and can relieve insomnia, anxiety, irritability and other emotional problems.

[0058] In the present application, the American ginseng is sweet and slightly bitter in taste, and cold in nature, and belongs to the lung, heart, kidney and spleen channels; it has the effects of tonifying the lung, heart, kidney and spleen, clearing heat and generating saliva, and clearing the heart and calming the nerves.

[0059] Compared with the prior art, the present application has the following beneficial effects:

[0060] 1. In the present application, the astragalus root, angelica, hawthorn, dried orange peel, mulberry, malt, dendrobium and American ginseng are used as components of the composition, and each component is synergistically matched, the prescription is simple, and the effects of tonifying qi and blood, calming the nerves, improving sleep and improving memory are brought.

[0061] 2. In the present application, the dendrobium is first decocted to destroy the tissue morphology, then the cellulase and pectinase are added to further decompose the cellulose and pectin on the cell wall, so that the effective components are dissolved in large quantities under mild conditions, and the activity of the effective components is not damaged, which is beneficial to the effects of improving sleep and memory; the astragalus root, angelica, malt and American ginseng components are ultrasonically decocted, which is beneficial to the dissolution of active ingredients and brings a significantly improved effect of tonifying qi and blood. DETAILED DESCRIPTION

[0062] The following non-limiting examples can make those skilled in the art more fully understand the present application, but do not limit the present application in any way. The following content is only an exemplary description of the scope of the present application, and those skilled in the art can make various changes and modifications to the present application according to the disclosed content, and it should also belong to the scope of the present application.

[0063] When the examples give numerical ranges, it should be understood that, unless otherwise specified by the present application, each numerical range has two endpoints and any number between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present application have the same meaning as generally understood by those skilled in the art to which the present application belongs.

[0064] The application will be further described in the following specific examples. The various chemical reagents and raw materials used in the examples of the application are obtained through conventional commercial channels unless otherwise specified.

[0065] Example 1

[0066] A composition for tonifying qi and blood, aiding sleep and improving memory, the raw material components according to weight parts are as follows: 16 parts of Radix Astragali, 12 parts of Angelica sinensis, 15 parts of Crataegus pinnatifida, 10 parts of Pericarpium Citri Reticulatae, 14 parts of Morus alba, 15 parts of Hordeum vulgare, 10 parts of Dendrobium nobile and 16 parts of Radix Panacis Quinquefolii.

[0067] The preparation method of the composition is as follows:

[0068] (1) Preparation of Dendrobium nobile extract: Dendrobium nobile was decocted with water at a solid-liquid ratio of 1:15 g / mL for 2 h to obtain a decoction mixture; the decoction mixture was cooled to 42℃, and a compound enzyme of cellulase and pectinase was added, and enzymolysis was carried out at 42℃ for 36 h to obtain the Dendrobium nobile extract; the mass ratio of cellulase and pectinase was 1:3, and the addition amount of the compound enzyme was 2 mg of compound enzyme per 200 mg of Dendrobium nobile;

[0069] (2) Radix Astragali, Angelica sinensis, Hordeum vulgare and Radix Panacis Quinquefolii were soaked in water, and ultrasonic treatment was carried out at a solid-liquid ratio of 1:15 g / mL for 1 h, and then decoction was carried out for 1 h, and filtration was carried out to obtain filtrate 1 and residue 1;

[0070] (3) Residue 1 was mixed with Crataegus pinnatifida, Pericarpium Citri Reticulatae and Morus alba, water was added, and decoction was continued at a solid-liquid ratio of 1:10 g / mL for 2 h, and filtration was carried out to obtain filtrate 2;

[0071] (4) Filtrate 1 and filtrate 2 were combined, concentrated, and freeze-dried to obtain a mixture; the mixture and the Dendrobium nobile extract were stirred uniformly to obtain the composition.

[0072] Example 2

[0073] A composition for tonifying qi and blood, aiding sleep and improving memory, the raw material components according to weight parts are as follows: 12 parts of Radix Astragali, 8 parts of Angelica sinensis, 12 parts of Crataegus pinnatifida, 8 parts of Pericarpium Citri Reticulatae, 13 parts of Morus alba, 12 parts of Hordeum vulgare, 8 parts of Dendrobium nobile and 13 parts of Radix Panacis Quinquefolii.

[0074] The preparation method of the composition is as follows:

[0075] (1) Preparation of Dendrobium nobile extract: Dendrobium nobile was decocted with water at a solid-liquid ratio of 1:8 g / mL for 3 h to obtain a decoction mixture, the decoction mixture was cooled to 38℃, and a compound enzyme of cellulase and pectinase was added, and enzymolysis was carried out at 38℃ for 48 h to obtain the Dendrobium nobile extract; the mass ratio of cellulase and pectinase was 1:3, and the addition amount of the compound enzyme was 2 mg of compound enzyme per 200 mg of Dendrobium nobile;

[0076] (2) Soak the Astragalus, Angelica, Malt and American ginseng in water, and ultrasonically treat them for 0.5 h at a solid-liquid ratio of 1:8 g / mL, then decoct them for 2 h, filter, and obtain filtrate 1 and residue 1;

[0077] (3) Mix residue 1 with Hawthorn, Tangerine peel and Mulberry, add water, and continue to decoct them for 1 h at a solid-liquid ratio of 1:6 g / mL, filter, and obtain filtrate 2;

[0078] (4) Combine filtrate 1 and filtrate 2, concentrate, and freeze-dry to obtain a mixture; mix the mixture with the Dendrobium extract to obtain the composition.

[0079] Example 3

[0080] A composition for tonifying qi and blood, aiding sleep and improving memory, the raw material components of which are as follows in terms of weight parts: 20 parts of Astragalus, 15 parts of Angelica, 20 parts of Hawthorn, 12 parts of Tangerine peel, 16 parts of Mulberry, 20 parts of Malt, 12 parts of Dendrobium and 20 parts of American ginseng.

[0081] The preparation method of the composition is as follows:

[0082] (1) Prepare the Dendrobium extract: decoct Dendrobium in water at a solid-liquid ratio of 1:20 g / mL for 1 h to obtain a decocting mixture, cool the decocting mixture to 45℃, add a compound enzyme of cellulase and pectinase, and enzymatically hydrolyze at 45℃ for 18 h to obtain the Dendrobium extract; the mass ratio of cellulase to pectinase is 1:3, and the addition amount of the compound enzyme is 1 mg of compound enzyme per 50 mg of Dendrobium;

[0083] (2) Soak the Astragalus, Angelica, Malt and American ginseng in water, and ultrasonically treat them for 2 h at a solid-liquid ratio of 1:20 g / mL, then decoct them for 0.5 h, filter, and obtain filtrate 1 and residue 1;

[0084] (3) Mix residue 1 with Hawthorn, Tangerine peel and Mulberry, add water, and continue to decoct them for 3 h at a solid-liquid ratio of 1:15 g / mL, filter, and obtain filtrate 2;

[0085] (4) Combine filtrate 1 and filtrate 2, concentrate, and freeze-dry to obtain a mixture; mix the mixture with the Dendrobium extract to obtain the composition.

[0086] Example 4

[0087] The difference between this example and Example 1 is that the weight parts of the raw materials of the composition are different.

[0088] A composition for tonifying qi and blood, aiding sleep and improving memory, the raw material components of which are as follows in terms of weight parts: 5 parts of Astragalus, 5 parts of Angelica, 5 parts of Hawthorn, 1 part of Tangerine peel, 5 parts of Mulberry, 5 parts of Malt, 1 part of Dendrobium and 1 part of American ginseng.

[0089] The rest is the same as in Example 1.

[0090] Example 5

[0091] The difference between Example 1 and Example 5 is that the weight parts of the raw materials of the composition are different.

[0092] A composition for tonifying qi and blood, aiding sleep, and improving memory, the raw material components of which are as follows in terms of weight parts: 50 parts of Astragalus, 30 parts of Angelica, 25 parts of hawthorn, 15 parts of dried tangerine or orange peel, 40 parts of mulberry, 25 parts of malt, 15 parts of dendrobium, and 25 parts of American ginseng.

[0093] The rest is the same as Example 1.

[0094] Example 6

[0095] The difference between Example 1 and Example 6 is that the mass ratio of cellulase and pectinase in the complex enzyme used for preparing the dendrobium extract in step (1) is different. The mass ratio of cellulase and pectinase is 1:1.2.

[0096] The rest is the same as Example 1.

[0097] Example 7

[0098] The difference between Example 1 and Example 7 is that the mass ratio of cellulase and pectinase in the complex enzyme used for preparing the dendrobium extract in step (1) is different. The mass ratio of cellulase and pectinase is 1:5.

[0099] The rest is the same as Example 1.

[0100] Comparative Example 1

[0101] The difference between Example 1 and Comparative Example 1 is that the raw material components of the composition are different.

[0102] A composition for tonifying qi and blood, aiding sleep, and improving memory, the raw material components of which are as follows in terms of weight parts: 16 parts of Astragalus, 15 parts of hawthorn, 10 parts of dendrobium, and 15 parts of malt.

[0103] The preparation method of the composition is as follows:

[0104] (1) Preparation of the dendrobium extract: dendrobium was decocted with water at a solid-liquid ratio of 1:15 g / mL for 2 h to obtain a decocting mixture, the decocting mixture was cooled to 42°C, and a complex enzyme of cellulase and pectinase was added, and the enzyme was hydrolyzed at 42°C for 36 h to obtain the dendrobium extract; the mass ratio of cellulase and pectinase was 1:3, and the addition amount of the complex enzyme was 2 mg of complex enzyme per 200 mg of dendrobium;

[0105] (2) Astragalus and malt were soaked with water, ultrasonically treated at a solid-liquid ratio of 1:15 g / mL for 1 h, decocted for 1 h, filtered to obtain filtrate 1 and residue 1;

[0106] (3) mix the residue 1 with hawthorn, add water, continue to decoct for 2 h at a solid-liquid ratio of 1:10 g / mL, filter to obtain filtrate 2;

[0107] (4) combine the filtrate 1 and the filtrate 2, concentrate, freeze-dry to obtain a mixture; mix the mixture and the dendrobium extract uniformly to obtain the composition.

[0108] Comparative Example 2

[0109] Different from Example 1, the raw material components of the composition are different.

[0110] A composition for tonifying qi and blood, aiding sleep and improving memory, the raw material components according to weight parts are as follows: 12 parts of angelica, 14 parts of mulberry, 10 parts of dried tangerine or orange peel and 16 parts of American ginseng.

[0111] The preparation method of the composition is as follows:

[0112] (1) soak the angelica and American ginseng in water, perform ultrasonic treatment for 1 h at a solid-liquid ratio of 1:15 g / mL, decoct for 1 h, filter to obtain filtrate 1 and residue 1;

[0113] (2) mix the residue 1 with dried tangerine or orange peel and mulberry, add water, continue to decoct for 2 h at a solid-liquid ratio of 1:10 g / mL, filter to obtain filtrate 2;

[0114] (3) combine the filtrate 1 and the filtrate 2, concentrate, freeze-dry to obtain the composition.

[0115] Comparative Example 3

[0116] Different from Example 1, the raw material components of the composition are different, and the American ginseng is replaced by poria.

[0117] A composition for tonifying qi and blood, aiding sleep and improving memory, the raw material components according to weight parts are as follows: 16 parts of milkvetch root, 12 parts of angelica, 15 parts of hawthorn, 10 parts of dried tangerine or orange peel, 14 parts of mulberry, 15 parts of malt, 10 parts of dendrobium and 16 parts of poria.

[0118] The preparation method of the composition is as follows:

[0119] (1) prepare a dendrobium extract: decoct the dendrobium in water at a solid-liquid ratio of 1:15 g / mL for 2 h to obtain a decocting mixture; cool the decocting mixture to 42℃, add a compound enzyme of cellulase and pectinase, and perform enzymolysis at 42℃ for 36 h to obtain the dendrobium extract; the mass ratio of cellulase to pectinase is 1:3, and the addition amount of the compound enzyme is 2 mg compound enzyme / 200 mg dendrobium;

[0120] (2) soak the milkvetch root, angelica, malt and poria in water, perform ultrasonic treatment for 1 h at a solid-liquid ratio of 1:15 g / mL, decoct for 1 h, filter to obtain filtrate 1 and residue 1;

[0121] (3) mixing the residue 1 with hawthorn, dried tangerine or orange peel and mulberry, adding water, and continuing to decoct for 2 h at a solid-liquid ratio of 1 : 10 g / mL to obtain a filtrate 2;

[0122] (4) combining the filtrate 1 and the filtrate 2, concentrating, and freeze-drying to obtain a mixture; and stirring the mixture and the dendrobe extract uniformly to obtain the composition.

[0123] Comparative Example 4

[0124] Different from Example 1, the weight ratio of the raw material components of the composition is different.

[0125] A composition for tonifying qi and blood, aiding sleep, and improving memory, the raw material components of which are 2 parts of astragalus, 12 parts of angelica, 35 parts of hawthorn, 20 parts of dried tangerine or orange peel, 23 parts of mulberry, 15 parts of malt, 0.5 part of dendrobe, and 0.5 part of American ginseng, in terms of weight.

[0126] The rest is the same as Example 1.

[0127] Comparative Example 5

[0128] Different from Example 1, the preparation of the dendrobe extract by enzymatic hydrolysis is not performed. The preparation method of the composition is as follows:

[0129] (1) soaking astragalus, angelica, malt, dendrobe, and American ginseng in water, and performing ultrasonic treatment for 1 h at a solid-liquid ratio of 1 : 15 g / mL, decocting for 1 h, and filtering to obtain a filtrate 1 and a residue 1;

[0130] (2) mixing the residue 1 with hawthorn, dried tangerine or orange peel, and mulberry, adding water, and continuing to decoct for 2 h at a solid-liquid ratio of 1 : 10 g / mL to obtain a filtrate 2;

[0131] (3) combining the filtrate 1 and the filtrate 2, concentrating, and freeze-drying to obtain the composition.

[0132] Comparative Example 6

[0133] Different from Example 1, in the preparation process of the dendrobe extract in step (1), only one component of cellulase is used. The preparation method of the composition is as follows:

[0134] (1) preparing a dendrobe extract: decocting dendrobe in water at a solid-liquid ratio of 1 : 15 g / mL for 2 h to obtain a decocting mixture; cooling the decocting mixture to 42℃, adding cellulase, and performing enzymatic hydrolysis at 42℃ for 36 h to obtain the dendrobe extract; the addition amount of cellulase is 2 mg of cellulase per 200 mg of dendrobe;

[0135] (2) Huangqi, Danggui, Maiya and Xiangyang were soaked in water, and then ultrasonic treatment was performed for 1 h at a solid-liquid ratio of 1:15 g / mL, decoction was performed for 1 h, and then filtration was performed to obtain filtrate 1 and residue 1;

[0136] (3) Residue 1 was mixed with Shanzha, Chenpi and Sangshen, water was added, and then decoction was performed for 2 h at a solid-liquid ratio of 1:10 g / mL, and then filtration was performed to obtain filtrate 2;

[0137] (4) Filtrate 1 and filtrate 2 were combined, concentrated, and then freeze-dried to obtain a mixture; the mixture and Dendrobium extract were uniformly stirred to obtain a composition.

[0138] Test 1: Effect detection of tonifying qi and blood and enhancing immunity

[0139] Experimental animals: 18-22 g female SPF Kunming mice. The mice were adaptively fed for 1 week, and the feeding temperature was 20-25 °C, and the feeding humidity was 40%-70% RH.

[0140] Experimental grouping: The mice were divided into 14 groups, 7 mice in each group, which were a control group, an example 1 group, an example 2 group, an example 3 group, an example 4 group, an example 5 group, an example 6 group, an example 7 group, a comparative example 1 group, a comparative example 2 group, a comparative example 3 group, a comparative example 4 group, a comparative example 5 group and a comparative example 6 group.

[0141] Administration method: Except for the control group, the mice in the remaining groups were orally administered with the corresponding composition every day, and the oral administration dose was 1.0 g / kg; the control group was orally administered with the same dose of distilled water. Once a day, for 30 consecutive days.

[0142] Experimental method:

[0143] (1) Measurement of half hemolysis value (HC 50 ): The mice were orally administered with the composition for 30 days, 2% SRBC was injected intraperitoneally on the 25th day, and the mice were sacrificed by dislocation after 5 days of immunization, serum was collected, 300-fold diluted serum 1 mL, 10% SRBC 0.5 mL, and complement 1 mL were added in a test tube, and then 20 minutes of 37 °C water bath, ice bath termination reaction, centrifugation, and then 1 mL of supernatant was added, 3 mL of Dows reagent was added, and then 10 minutes of 540 nm colorimetry. The detection results of half hemolysis value (HC 50 ) are shown in Table 1.

[0144] (2) Antibody-producing cell detection: the spleen of the mouse killed in (1) was taken, the cell suspension was supported, filtered through a 200-mesh screen, mixed with Han's solution, and distributed into small test tubes, 0.5 mL per tube, 50 μL of 10% SRBC was added, 20 μL of the spleen cell suspension was added, mixed, and placed in a carbon dioxide incubator for 1 hour, complement diluted with SA buffer (sulfosalicylic acid buffer) (1:10) was added, and incubation was continued for 1 hour, and the number of hemolytic plaques was counted. The results of the hemolytic plaque number are shown in Table 1.

[0145] Table 1

[0146] HC 50 ]] Number of hemolytic plaques (x 10 3 / total spleen) Control group 221.8±11.5 55.4±5.1 Example 1 273.9 ± 10.2 ## ]] 69.4 ± 5.5 ## ]] Example 2 269.2 ± 11.0 ## ]] 69.0 ± 6.6 ## ]] Example 3 267.7 ± 9.7 ## ]] 68.6 ± 6.0 ## ]] Example 4 257.4 ± 10.7 ## ]] 66.8 ± 4.8 ## ]] Example 5 261.6 ± 9.6 ## ]] 67.1 ± 5.4 ## ]] Example 6 265.0 ± 10.3 ## ]] 68.2 ± 3.9 ## ]] Example 7 268.5 ± 11.3 ## ]] 68.5 ± 4.5 ## ]] Comparative Example 1 240.6 ± 8.1 △ ]] 59.9 ± 5.3 △△ ]] Comparative Example 2 232.7 ± 8.3 △△ ]] 58.8 ± 6.2 △△ ]] Comparative Example 3 246.8 ± 7.8 △ ]] 60.1 ± 3.5 △△ <!-- 8 -->]] Comparative Example 4 244.2 ± 10.1 △ ]] 60.3 ± 4.4 △△ ]] Comparative Example 5 246.1 ± 12.0 △ ]] 62.3 ± 5.2 △ ]] Comparative Example 6 250.3 ± 9.5 # ]] 63.4 ± 4.9 △ ]]

[0147] In Table 1, # P < 0.05, ## P < 0.01, each experimental group has a significant difference compared with the control group; △ P < 0.05, △△ P < 0.01, the comparative group has a significant difference compared with the Example 1 group;

[0148] As can be seen from Table 1, the composition of the present application can significantly improve the HC 50 and the number of hemolytic plaques, and has a significant difference compared with the immune effect of the composition of the comparative example.

[0149] After changing the raw material components of the compositions of Comparative Examples 1-3, the synergistic effect between the compositions is destroyed due to the different components, and the immune effect is significantly reduced; especially, the components of Comparative Example 1 are only 4 kinds, and the components of Comparative Example 2 are only 4 kinds, even if the effects are added, it still cannot reach the effect brought by the whole of 8 components.

[0150] After changing the weight ratio of the components of the composition of Comparative Example 4, the HC 50 and the number of hemolytic plaques are significantly reduced; after changing the preparation method of the Dendrobium extract in Comparative Examples 5 and 6, the active components of the Dendrobium extract are changed, thereby affecting the HC 50 and the number of hemolytic plaques.

[0151] Test 2 sleep aid and memory improvement effect detection

[0152] Experimental animals: Wild-type AB strain zebrafish, according to the Zebrafish Book on the management method of zebrafish, feeding, spawning and daily management. The same batch of 18-month-old zebrafish was selected for the experiment, placed in a centralized zebrafish independent circulating water system, the water temperature was maintained at about 28.5℃, pH = 7, the light and dark conditions were set to 14 hours of light (9:00-23:00 every day) and 10 hours of darkness (23:00 at night-9:00 the next morning). Feed twice a day, food is abundant shrimp and fish feed. The breeding and management of zebrafish are implemented in accordance with the "Regulations of the People's Republic of China on the Management of Experimental Animals".

[0153] Experimental animal grouping and modeling: The same batch of normally developed old zebrafish was selected for the experiment, and after adaptive feeding for 7 days, they were randomly divided into a blank control group, a model group, an example 1 group, an example 2 group, an example 3 group, an example 4 group, an example 5 group, an example 6 group, an example 7 group, a comparative example 1 group, a comparative example 2 group, a comparative example 3 group, a comparative example 4 group, a comparative example 5 group and a comparative example 6 group, a total of 15 groups. 21 zebrafish in each group.

[0154] The blank control group was normally fed with a 14h light and 10h dark cycle; the other experimental groups were given continuous light for 3d to model sleep deprivation; at the same time, the example groups and the comparative example groups were immersed in solutions containing the composition, and each group replaced half of the solution every day and supplemented with new solution, and the turbidity in the fish tank was cleaned.

[0155] The concentration of the composition in the feeding solution of the example groups and the comparative example groups was 200mg / L.

[0156] Experimental method: After the light ended, the sleep behavior and learning and memory ability of each group of zebrafish were detected. Among them, 6 zebrafish were randomly selected from each group for sleep behavior detection, and the remaining 15 zebrafish were used for learning and memory ability detection.

[0157] (1) Sleep behavior detection

[0158] During the experiment, 6 zebrafish were placed in 6 fish tanks with a diameter of 7cm, fish water was added to the fish tank to 1 / 2 of the height of the fish tank, and the zebrafish could move freely in the tank. The camera system on the behavior analysis system automatically tracked the movement of the zebrafish and analyzed it, and the tracking time was 48h. After 48h of tracking, the resting time and total movement distance of the zebrafish were recorded.

[0159] (2) Learning and memory ability detection

[0160] The learning and memory ability of zebrafish was observed by using a zebrafish T maze behavior tracking system. The T maze was composed of left and right arms of equal length and a vertical channel. The depth of the maze was 10 cm, and the filling height was 8 cm. In the middle arm of the maze, a 10 cm x 10 cm x 10 cm area was closed to form a starting box. Three appropriate size partitions were used to isolate the starting box, the left arm and the right arm of the maze. In the T maze test, the zebrafish only received food during the daily training period, and the right arm was connected with the enriched chamber (EC) area. The T maze test was performed according to the method (an article published by Benvenutti R et al.). The day before the test, the zebrafish was given a 2-hour adaptation period to freely move in the T maze, so as to reduce the disturbance of the new environment on the behavior of the zebrafish.

[0161] On the test day, the zebrafish to be tested was placed in the starting box, and the zebrafish was given 30 seconds to adapt to the new environment in the starting box. After the adaptation period ended, the starting box baffle was removed. The zebrafish was allowed to freely move in the T maze, and the latency time of the fish from the starting point to find the EC area was observed and recorded (when the fish found and stayed in the EC area for 30 seconds, it was considered to enter the EC area). The test time was 6 minutes. The zebrafish that did not find the EC area was guided to enter the EC area and stay for 30 seconds after 6 minutes. After 3 days of intervention, the latency time of the zebrafish entering the EC area after 3 days of intervention was recorded.

[0162] The resting time, total movement distance and latency time of the zebrafish entering the EC area are shown in Table 2.

[0163] Table 2

[0164] Total resting time (s) Total distance of movement (mm) Latency time to enter EC area (s) Blank control group 115972 ± 10665 ☆☆ ]] 126584 ± 25915 ☆☆ ]] 58.17 ± 25.05 ☆☆ ]] Model group 88521 ± 8735 ## ]] 168997 ± 30523 ## ]] 167.51 ± 58.17 ## ]] Example 1 129588 ± 11282 ☆☆ ]] 95623 ± 9907 ☆☆ ]] 60.28 ± 31.52 ☆☆ ]] Example 2 128542 ±9824 ☆☆ ]] 99829 ± 7885 #☆☆ ]] 62.57 ± 27.56 ☆☆ ]] Example 3 126541 ±9757 ☆☆ ]] 102547 ± 10146 #☆☆ ]] 62.65 ± 19.58 ☆☆ ]] Example 4 117529 ± 9660 ☆☆ ]] 109826 ±9735 #☆☆ ]] 64.13 ± 26.97 ☆☆ ]] Example 5 122032 ± 9328 ☆☆ ]] 107369 ± 9684 #☆☆ ]] 63.47 ± 24.14 ☆☆ ]] Example 6 125000 ± 10119 ☆☆ ]] 101698 ±9227 #☆☆ ]]> 61.98 ± 30.85 ☆☆ ]] Example 7 127504 ± 8537 ☆☆ ]] 101536 ± 10205 #☆☆ ]] 62.05 ± 29.30 ☆☆ ]] Comparative Example 1 103550 ± 8641 #☆△△ ]] 143525 ± 26712 #☆△ ]] 115.76 ± 28.83 ##☆△△ ]] Comparative Example 2 95968 ± 11725 #☆△△ ]] 149894 ± 21480 #☆△ ]] 132.42 ± 33.69 ##☆△△ ]] Comparative Example 3 110013 ± 12067 ☆△ ]] 136068 ±22831 ☆△ ]]> 90.57 ± 34.70 #☆△ ]] Comparative Example 4 109326 ± 9953 ☆△ ]] 136987 ± 23622 ☆△ ]] 95.07 ± 38.12 #☆△ ]] Comparative Example 5 110588 ± 10290 ☆△ ]] 128290 ± 5564 ☆☆△ ]] 85.18 ± 25.34 ☆☆△ ]] Comparative Example 6 115064 ±9199 ☆△ ]]> 120564 ± 12339 ☆☆△ ]]> 79.85 ± 19.65 ☆☆△ ]]

[0165] In Table 2, # P<0.05, ## P<0.01, each experimental group was significantly different from the blank control group; ☆ P<0.05, ☆☆ P<0.01, each experimental group was significantly different from the model group; △ P<0.05, △△ P<0.01, the comparative group was significantly different from the example 1 group.

[0166] As can be seen from Table 2, the composition of the application can significantly improve the resting time, reduce the total movement distance and reduce the latency time of entering the EC area. The composition of the application has a significant effect on improving the movement behavior of sleep-deprived zebrafish and improving learning and memory.

[0167] After the raw material components of the compositions of Comparative Examples 1-3 were changed, the effects of sleep improvement and learning and memory improvement were significantly reduced; in particular, Comparative Example 1 had only 4 components and Comparative Example 2 had only 4 components. Even if the effects were added together, they could not achieve the specific improvement effect brought about by the overall composition of the 8 components of this invention.

[0168] Although the composition of Comparative Example 4, after altering the weight ratio of its components, exhibited some effects in improving sleep and enhancing memory, its effects were still significantly different from those of the composition of this invention. Comparative Examples 5 and 6, by changing the preparation method of the Dendrobium extract, affected the resting time, total movement distance, and latency to enter the EC zone in zebrafish.

[0169] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A composition for tonifying qi and blood, aiding sleep and improving memory, characterized in that, The raw materials consist of the following components by weight parts: 5-50 parts of Astragalus, 5-30 parts of Angelica, 5-25 parts of hawthorn, 1-15 parts of dried tangerine or orange peel, 5-40 parts of mulberry, 5-25 parts of malt, 1-15 parts of dendrobium and 1-25 parts of American ginseng; The composition is prepared by a preparation method comprising the following steps: (1) preparing a dendrobium extract: boiling dendrobium with water to obtain a boiling mixture; adding a compound enzyme to the boiling mixture after cooling for enzymolysis to obtain the dendrobium extract; the compound enzyme is cellulase and pectinase; (2) soaking Astragalus, Angelica, malt and American ginseng in water, ultrasonic treatment, boiling, filtering to obtain filtrate 1 and residue 1; (3) mixing residue 1 with hawthorn, dried tangerine or orange peel and mulberry, continuing to boil with water, filtering to obtain filtrate 2; (4) combining filtrate 1 and filtrate 2, concentrating and drying to obtain a mixture; mixing the mixture and the dendrobium extract to obtain the composition.

2. The composition of claim 1, wherein, The raw materials consist of the following components by weight parts: 12-20 parts of Astragalus, 8-15 parts of Angelica, 12-20 parts of hawthorn, 8-12 parts of dried tangerine or orange peel, 13-16 parts of mulberry, 12-20 parts of malt, 8-12 parts of dendrobium and 13-20 parts of American ginseng.

3. The composition of claim 2, wherein, The raw materials consist of the following components by weight parts: 16 parts of Astragalus, 12 parts of Angelica, 15 parts of hawthorn, 10 parts of dried tangerine or orange peel, 14 parts of mulberry, 15 parts of malt, 10 parts of dendrobium and 16 parts of American ginseng.

4. Process for the preparation of a composition according to any one of claims 1 to 3, characterized in that, The method comprises the following steps: (1) preparing a dendrobium extract: boiling dendrobium with water to obtain a boiling mixture; adding a compound enzyme to the boiling mixture after cooling for enzymolysis to obtain the dendrobium extract; the compound enzyme is cellulase and pectinase; (2) soaking Astragalus, Angelica, malt and American ginseng in water, ultrasonic treatment, boiling, filtering to obtain filtrate 1 and residue 1; (3) mixing residue 1 with hawthorn, dried tangerine or orange peel and mulberry, continuing to boil with water, filtering to obtain filtrate 2; (4) combining filtrate 1 and filtrate 2, concentrating and drying to obtain a mixture; mixing the mixture and the dendrobium extract to obtain the composition.

5. The preparation method according to claim 4, characterized in that, In step (1), the solid-liquid ratio of boiling is 1:8-20 g / mL, and the boiling time is 1-3 h; the cooling temperature is 38-45℃.

6. The preparation method according to claim 4, characterized in that, In step (1), the mass ratio of cellulase and pectinase is 1:1.2-5; the adding amount of the compound enzyme is 1-3 mg compound enzyme / 50-300 mg dendrobium.

7. The preparation method according to claim 4, characterized in that, In step (1), the enzymolysis temperature is 38-45℃, and the enzymolysis time is 18-48 h.

8. The preparation method according to claim 4, characterized in that, In step (2), the solid-liquid ratio of soaking in water is 1:8-20 g / mL, the ultrasonic treatment time is 0.5-2 h, and the boiling time is 0.5-2 h; In step (3), the solid-liquid ratio of boiling is 1:6-15 g / mL, and the boiling time is 1-3 h.

9. The use of a composition in the preparation of a medicine having the effects of replenishing qi and blood, aiding sleep, and improving memory, characterized in that, The composition is the composition of any one of claims 1-3 or the composition prepared by the preparation method of any one of claims 4-8.

10. A medicament having the effects of tonifying qi and blood, aiding sleep and improving memory, characterized in that, The composition comprises the composition of any one of claims 1-3 or the composition prepared by the preparation method of any one of claims 4-8.