Automatic pretreatment device for sample preparation for aflatoxin detection

Abstract

The invention relates to the technical field of aflatoxin pollution detection, in particular to an automatic pretreatment device for sample preparation for aflatoxin detection, which comprises a crushing and extracting part and an enriching and purifying part. The device can also be divided into a gas path part, a liquid path part and a control part, and the gas path part provides pressure for the liquid path part under the sequential regulation and control of the control part so as to complete the liquid adding and discharging of various liquids. The whole device works cooperatively, so that the work of crushing the sample, quantitatively adding / discharging the extracting solution, quantitatively adding / discharging the diluent, quantitatively adding / discharging the leacheate, quantitatively adding / discharging the eluent, collecting the waste liquid, collecting the supernate and the like can be realized. Specifically, extraction, enrichment purification and collection of AFB1 in a grain sample polluted by aflatoxin can be realized, and the efficiency of the sample preparation process can be effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of aflatoxin pollution detection, and particularly to an automatic pretreatment device for sample preparation of aflatoxin detection. Background Art

[0002] Cereals are the most important food and energy sources, but fungi and mycotoxins are widely present in foods and feeds, which has become one of the main hazards affecting food quality and safety. According to the data of the Food and Agriculture Organization of the United Nations (FAO), about one-fourth of the world's food and feed are threatened by mycotoxin contamination every year. Infection with Aspergillus flavus ( Aspergillus flavus Link) will consume the nutrients of the object, damage the physical properties and reduce its quality, and even produce aflatoxin (AFT). Among all the mycotoxins in cereals, aflatoxin B1 (AFB1) has the greatest toxicity and is recognized as a Class 1 carcinogen by the World Health Organization (WHO). The famous aflatoxin outbreaks include "rural areas of Kenya in 2004" and "Disease X in Turkey", and the source of the danger is the maize and peanut meal contaminated with aflatoxin. Currently, maize has surpassed rice and wheat to become the most important cereal in terms of production. It is not only used as human food, but also converted into a series of value-added foods, feeds, industrial products and fuel bioethanol, and has high edible and economic value worldwide.

[0003] There are many technologies and measures for mycotoxin detection, such as usually using chemical detection methods such as high performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA). These conventional chemical detection methods are technically mature and have high detection accuracy, but the disadvantages are that they are destructive, labor-intensive and time-consuming with low efficiency. The detection instruments for toxin content detection are quite mature, such as high performance liquid chromatographs and enzyme label instruments. However, due to the limitations of detection technologies, most cereals and foods vulnerable to mycotoxin contamination cannot directly analyze aflatoxin without extraction and cleaning. However, extraction and cleaning often go through complex steps, that is, the main link of time-consuming and reducing detection efficiency is the sample preparation (i.e., pretreatment process) link before sample detection. In view of this, designing an automatic pretreatment device for sample preparation of aflatoxin detection can realize the automatic processing of the sample preparation process for aflatoxin detection in the laboratory. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide an automatic pretreatment device for sample preparation of aflatoxin detection that can realize the automatic processing of the sample preparation process for aflatoxin detection in the laboratory, so as to solve the problems in the above background art.

[0005] To solve the above problems, the technical solution adopted by the present invention is: designing an automated pretreatment device for sample preparation in aflatoxin detection, which can realize the extraction, enrichment and purification, and collection of the substances to be detected in the sample. It includes a crushing and extraction part and an enrichment and purification part; The crushing and extraction part can realize the addition of the extraction liquid and the extraction of the sample supernatant. It mainly includes an extraction container Ⅰ. There is a rotatable crushing knife at the bottom of the container Ⅰ, and there are liquid addition channels and filtrate channels on the container wall. After the extraction liquid is added to the container Ⅰ, the crushing knife starts to crush and stir to achieve the crushing and mixing of the sample and extraction, and after the crushing is completed, all / part of the extraction liquid enters the next link; The enrichment and purification part can realize the dilution, enrichment and purification of the extraction liquid. Dilution is achieved by adding a dilution liquid to the closed container Ⅱ. The diluted liquid passes through the analyte capture area connected to the container Ⅱ and then flows into the container Ⅳ for collecting waste liquid through the channel Ⅰ. The diluted liquid flows out to the container Ⅳ after passing through the immunoaffinity column or immunomagnetic beads; by adding a washing liquid to the container Ⅱ and making it pass through the immunoaffinity column or immunomagnetic beads again and then flowing out through the channel Ⅰ to the container Ⅳ, the immunoaffinity column or immunomagnetic beads are washed to remove impurities; after washing, an elution liquid is added to the immunoaffinity column or immunomagnetic beads, and the captured analyte is eluted and flows out through the channel Ⅱ to the container Ⅱ; The automated pretreatment device for sample preparation in aflatoxin detection can realize the crushing of the sample, the quantitative addition / removal of the extraction liquid, the quantitative addition / removal of the dilution liquid, the quantitative addition / removal of the washing liquid, the quantitative addition / removal of the elution liquid, the collection of waste liquid, and the collection of supernatant.

[0006] As a further improvement of the present invention, the addition of the extraction liquid, dilution liquid, washing liquid and elution liquid is realized by a liquid pump / gas pump.

[0007] As a further improvement of the present invention, the dilution liquid and the washing liquid adopt the same liquid.

[0008] As a further improvement of the present invention, the downward outlet of the immunoaffinity column or immunomagnetic beads has two channels. The channel Ⅰ is the common channel for the dilution liquid and the washing liquid to flow out after passing through the immunoaffinity column or immunomagnetic beads, and the channel Ⅱ is the collection channel for the elution liquid to flow out after passing through the immunoaffinity column or immunomagnetic beads.

[0009] As a further improvement of the present invention, it includes an air circuit part, a liquid circuit part, and a control part. The air circuit part provides pressure for the liquid circuit part under the sequential control of the control part to complete the addition and discharge of various liquids; The air circuit part mainly includes an air filter, a gas pump and a plurality of two-way two-position solenoid valves; the gas pump is a positive pressure pump, which mainly provides a positive pressure to each liquid storage device, extraction device and dilution device to achieve the air-liquid conversion to achieve the effect of liquid addition and drainage; The two-position two-way solenoid valve is in the normally closed position and can connect the gas pipeline when energized. When the solenoid valve is energized, the corresponding flow path pipeline can be connected.

[0010] As a further improvement of the present invention, the liquid path part includes an extraction liquid storage unit, a diluent / rinse solution memory, an eluent memory, container I, container II, a target capture area, container IV, and container III; the extraction liquid memory, container I, container II, the diluent / rinse solution memory, and the eluent memory are respectively connected to the pipeline through two-position two-way solenoid valves, and the gas source is used as the power source; As a further improvement of the present invention, the extraction liquid memory and container I, between container I and container II, and between the diluent / rinse solution memory and container II are all connected through liquid path pipelines. Check valves are also respectively connected to the pipelines to prevent liquid backflow. A two-position two-way solenoid valve is also provided between container I and container II, which can connect and cut off the circuit between the two containers.

[0011] As a further improvement of the present invention, the upstream of the target capture area is connected to container II, and two outlets are arranged downstream and are respectively connected to container IV and container III.

[0012] As a further improvement of the present invention, the target capture area sequentially includes a two-position two-way solenoid valve, an immunoaffinity column, and a two-position three-way solenoid valve; the normal position of the two-position two-way solenoid valve is the left position with normal disconnection, and the right position is connected after being energized. The upstream is connected to container II, and the downstream is connected to the inlet of the immunoaffinity column.

[0013] As a further improvement of the present invention, the downstream of the immunoaffinity column is a two-position three-way solenoid valve with the left position connected in the normal position. The downstream of the two-position three-way solenoid valve is connected to container IV; when energized, the two-position three-way solenoid valve becomes the right position, and the downstream is connected to container III; a liquid path pipeline is connected between the two-position two-way solenoid valve and the immunoaffinity column and is connected to the eluent memory, and a check valve is connected to the liquid path pipeline.

[0014] The beneficial effects of adopting the above technical solutions are as follows: The automatic pretreatment device for sample preparation in the detection of aflatoxin described in the present invention can automatically process the processes after sample weighing and before machine detection. Specifically, it can realize the extraction, enrichment purification, and collection of AFB1 in contaminated cereal samples with aflatoxin, and can effectively improve the efficiency of the sample preparation process. Description of the Drawings

[0015] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the following drawings are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0016] Figure 1 It is a schematic diagram of the principle of the present invention.

[0017] Figure 2 It is a schematic diagram of the structure of the whole machine of the present invention.

[0018] Figure 3 It is a schematic diagram of the structure of the whole machine of the present invention at Angle I.

[0019] Figure 4 It is a schematic diagram of the structure of the whole machine of the present invention at Angle II.

[0020] Figure 5 It is a schematic diagram of the structure of the whole machine of the present invention at Angle III.

[0021] Wherein: 1 - crushing and extraction device; 2 - front housing; 3 - immunoaffinity column; 4 - power switch; 5 - container III; 6 - container IV; 7 - power plug; 8 - gas source; 9 - program start switch; 10 - support plate; 11, 16 - two-position two-way solenoid valves; 12 - buzzer; 13 - two-position three-way solenoid valve; 14 - bottom plate; 15 - connecting pipe; 17 - eluent container bottle; 18 - diluent / rinse solution container bottle; 19 - extract container bottle; 20 - filter; 21 - collector box; 22 - connecting wire; 23 - rear housing; 24 - check valve; 25 - container II. Specific embodiments

[0022] To make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be clearly and completely described below in conjunction with specific embodiments. It should be understood that the orientation or positional relationships indicated by the terms "center", "vertical", "horizontal", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. Specific embodiment 1: As Figure 1 shown, an automated pretreatment device for sample preparation in the detection of aflatoxin can automate the processing process after sample weighing and before machine detection, and can realize the extraction, enrichment purification and collection of the analyte in the sample. It includes a crushing and extraction part and an enrichment purification part. The crushing and extraction part can realize the addition of the extraction solution and the extraction of the sample supernatant. It mainly includes an extraction container I. There is a rotatable crushing knife at the bottom of the container I, and there are liquid addition channels and filtrate channels on the container wall. After the extraction solution is added to the container I, the crushing knife starts to crush and stir to achieve the crushing and mixing of the sample and extraction. After the crushing is completed, all / part of the extraction liquid enters the next step.

[0024] The enrichment and purification part can realize the dilution, enrichment and purification of the extract. Dilution is achieved by adding diluent to the closed container II. The diluted liquid flows into the waste liquid collection container IV through the analyte capture area connected to container II and then through channel I. The diluted liquid flows out into container IV after passing through the immunoaffinity column or immunomagnetic beads. By adding eluent to container II and making it pass through the immunoaffinity column or immunomagnetic beads again and then flow out through channel I into container IV, the immunoaffinity column or immunomagnetic beads are eluted to wash away impurities. After elution, eluent is added to the immunoaffinity column or immunomagnetic beads, and the captured analyte is eluted and flows out through channel II into container II.

[0025] The automated pretreatment device for sample preparation in aflatoxin detection can realize sample crushing, quantitative addition / removal of extract, quantitative addition / removal of diluent, quantitative addition / removal of eluent, quantitative addition / removal of eluate, waste liquid collection, and supernatant collection. The addition of extract, diluent, eluent and eluate is achieved by liquid pump / air pump. The diluent and eluent use the same liquid. The downward outlets of the immunoaffinity column or immunomagnetic beads are two channels. Channel I is the common channel for the diluent and eluent to flow out after passing through the immunoaffinity column or immunomagnetic beads, and channel II is the collection channel for the eluate to flow out after passing through the immunoaffinity column or immunomagnetic beads.

[0026] The automated pretreatment device for sample preparation in aflatoxin detection includes an air circuit part, a liquid circuit part, and a control part. The air circuit part provides pressure for the liquid circuit part under the sequential control of the control part to complete the addition and discharge of various liquids. The air circuit part mainly includes an air filter, an air pump and a plurality of two-way two-position solenoid valves; the air pump is a positive pressure pump, which mainly provides positive pressure to each liquid storage device, extraction device and dilution device to achieve gas-liquid conversion to achieve the effect of liquid addition and discharge. The two-way two-position solenoid valve is in the normally closed position and can connect the gas pipeline when energized, and the solenoid valve can connect the corresponding flow pipeline when energized.

[0027] The liquid circuit part includes an extract storage unit, a diluent / eluent storage device, an eluate storage device, container I (crushing and extraction device), container II (filtered liquid collection container), target capture area, container IV (waste liquid collection container) and container III (eluate collection bottle). The extract storage unit, container I (crushing and extraction device), container II (filtered liquid collection container), diluent / eluent storage device, and eluate storage device are respectively connected to the pipeline through two-way two-position solenoid valves, with the gas source as the power source.

[0028] There are liquid pipelines connecting the extraction solution storage and Container I (crushing extraction device), Container I (crushing extraction device) and Container II (filtrate collection container), and the diluent / rinse solution storage and Container II (filtrate collection container). Check valves are respectively connected to the pipelines to prevent liquid backflow. A two-position two-way solenoid valve is also set between Container I (crushing extraction device) and Container II (filtrate collection container) to connect and cut off the loop between the two containers.

[0029] Upstream of the target capture area is connected to Container II (filtrate collection container), and there are two outlets downstream respectively connected to Container IV (waste liquid collection container) and Container III (eluate collection bottle). The target capture area sequentially includes a two-position two-way solenoid valve, an immunoaffinity column, and a two-position three-way solenoid valve; the normal position of the two-position two-way solenoid valve is the left-position normally closed position, and when powered on, the right position is connected. The upstream is connected to Container II (filtrate collection container), and the downstream is connected to the inlet of the immunoaffinity column.

[0030] Downstream of the immunoaffinity column is a two-position three-way solenoid valve with the normal position of the left side connected. The downstream of the two-position three-way solenoid valve is connected to Container IV (waste liquid collection container); when powered on, the two-position three-way solenoid valve becomes the right position, and the downstream is connected to Container III (eluate collection bottle); there is a liquid pipeline connecting between the two-position two-way solenoid valve and the immunoaffinity column and the eluate storage, and a check valve is connected to the liquid pipeline. Specific Embodiment 2: As Figures 2 - 5 This is another embodiment of the present invention. In this embodiment, the main body and the shell of the entire pretreatment device include a bottom plate 14, a support plate 10 vertically arranged on the bottom plate 14, and a front shell 2 and a rear shell 23 that can be opened and closed are hinged on both sides of the support plate 10. Sequentially connected on one side of the support plate 10 close to the front shell 2 are a crushing extraction device 1, a Container II 25, and an immunoaffinity column 3. Container II 25 is a filtrate collection container. A filter 20, a check valve 24, and a two-position two-way solenoid valve 11 are arranged between the crushing extraction device 1 and the inlet of Container II 25. A two-position two-way solenoid valve 11 is arranged between the outlet of Container II 25 and the immunoaffinity column 3. The outlet of the immunoaffinity column 3 is connected to a two-position three-way solenoid valve 13. One of the two outlets of the two-position three-way solenoid valve 13 is connected to Container III 5 and the other is connected to Container IV 6. Container III 5 and Container IV 6 are respectively an eluate collection bottle and a waste liquid collection container for collecting waste liquid and eluate. The two-position two-way solenoid valve is in the normally closed position and can allow liquid or gas to flow through when powered on. The two-position three-way solenoid valve 13 is in the normally open position, connecting to the waste liquid collection container when not powered on, and connecting to the eluate collection bottle when powered on.

[0032] On one side of the support plate 10 close to the rear housing 23, there are an eluent container bottle 17, a diluent / rinsing solution container bottle 18, an extract container bottle 19, and a power collection box 21. Above the three container bottles, there are gas circuit and liquid circuit channels. On the gas circuit channel, two-position two-way solenoid valves 16 are respectively arranged and connected to the gas source 8. On the liquid circuit channel, check valves 24 are arranged. When pressurizing the three container bottles, the liquid in the bottles can flow out along the pipelines extending to the bottom of the bottles through the check valves 24 and be added to other specified containers. After stopping pressurization, the check valves 24 prevent the liquid from flowing back into the container bottles. The gas source 8 is also connected to the crushing and extraction device 1, and a two-position two-way solenoid valve 16 is also arranged between them. The two-position two-way solenoid valve is turned on when powered on and closed when powered off.

[0033] The extract container bottle 19 is communicated with the crushing and extraction device 1, and the eluent container bottle 17 and the diluent / rinsing solution container bottle 18 are respectively communicated with the container II 25 (filtrate collection container). In the power collection box 21, there are mainly a control board for controlling the start of the solenoid valves and the gas source of the whole device and a relay. The control board of the present invention uses an Arduino open-source single-chip microcomputer for sequential control. A power switch 4, a program start switch 9, and a buzzer 12 are also provided.

[0034] The specific implementation process is as follows: Taking the corn kernels contaminated with aflatoxin as an example, first weigh a single corn kernel or weigh 5 g of corn kernels and put them into the crushing and extraction device 1. Then, the control part starts the crushing knife of the crushing and extraction device 1 to crush the kernels (single or multiple crushing). Then, turn on the solenoid valve corresponding to the extract container bottle 19 to quantitatively add the extract (generally methanol aqueous solution or acetonitrile aqueous solution) into the crushing and extraction device 1. The control part starts the crushing knife of the crushing and extraction device 1 to continue to start the extraction of aflatoxin in the crushed material. After the crushing knife rotates in a cycle for single or multiple times, it stops. Subsequently, after standing, connect the two-position two-way solenoid valve connecting the gas source 8 and the crushing and extraction device 1 to add a positive pressure, so that all or part of the extract is filtered out into the container II 25 (filtrate collection container). During the filtering process, the corresponding two-position two-way solenoid valve on the container II 25 is started. After the filtering is completed, the two two-position two-way solenoid valves are closed.

[0035] When the filtrate needs to be diluted, the air source 8 and the corresponding two-way two-position solenoid valve are used to pressurize the diluent / rinse solution container bottle 18, so that the liquid in the diluent / rinse solution container bottle 18 is quantitatively added to the container II 25 to complete the dilution of the filtrate. Subsequently, the two-way two-position solenoid valve connecting the air source 8 and the container II 25 (filtrate collection container) is turned on to apply a positive pressure, and the diluted liquid flows into the container IV 6 (waste liquid collection container) through the two-way two-position solenoid valve downstream of the container II 25, the immunoaffinity column 3, and the two-way three-position solenoid valve 13. At this time, the two-way two-position solenoid valve downstream of the container II 25 is also energized. The immunoaffinity column 3 here is replaceable, and the used immunoaffinity column 3 is an activated column. This step captures the substance to be detected, aflatoxin, on the immunoaffinity column 3 to complete the enrichment and purification of the substance to be detected such as AFB1.

[0036] After the filtrate is drained, the air source 8 and the corresponding two-way two-position solenoid valve are used to pressurize the diluent / rinse solution container bottle 18, so that the liquid in the diluent / rinse solution container bottle 18 is quantitatively added to the container II 25, and then flows through the immunoaffinity column 3 into the container IV 6 (waste liquid collection container). This step is to wash the impurities in the immunoaffinity column 3. At this time, the two-way two-position solenoid valve downstream of the container II 25 is also energized. The diluent / rinse solution can be an aqueous solution or a PBS buffer solution. In this embodiment, an aqueous solution is used.

[0037] Next, the air source 8 and the corresponding two-way two-position solenoid valve connected to the eluent container bottle 17 are used to pressurize the eluent container bottle 17, so that the liquid in the eluent container bottle 17 adds an eluent (generally 1-2 mL of methanol) to the container II 25. Then the corresponding two-way two-position solenoid valve is de-energized, and the air source 8 and the corresponding two-way two-position solenoid valve connected to the container II 25 are started to apply a positive pressure to the container II 25, so that the eluent flows along the two-way two-position solenoid valve between the container II 25 and the immunoaffinity column 3, the immunoaffinity column 3, and the two-way three-position solenoid valve 13 (energized at this time) into the container III 3 (eluent collection bottle) to complete the collection of the eluent.

[0038] If the eluent needs to be diluted, the air source 8 and the corresponding two-way two-position solenoid valve are used to pressurize the diluent / rinse solution container bottle 18, so that the liquid in the diluent / rinse solution container bottle 18 is quantitatively added to the container II 25, and the diluent flows along the two-way two-position solenoid valve between the container II 25 and the immunoaffinity column 3, the immunoaffinity column 3, and the two-way three-position solenoid valve 13 (energized at this time) into the container III 3 (eluent collection bottle) to complete the dilution of the eluent. After all steps are completed, the buzzer 12 rings and stops after 3 seconds of reminder. After the obtained eluent is manually filtered, the aflatoxin content can be directly detected by a high performance liquid chromatograph (HPLC). Here, the filtration is generally needle filter filtration or filter paper filtration.

[0039] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An automated sample preparation pre-treatment device for aflatoxin detection, characterized in that: It can realize the extraction, enrichment, purification and collection of the objects to be detected in the sample, which includes a crushing and extraction part and an enrichment and purification part; The crushing and extraction part can realize the addition of the extract and the extraction of the sample supernatant, and mainly includes an extraction container I. The bottom of the container I has a rotatable crushing knife, and the container wall has a liquid addition channel and a filtrate channel. After the extract is added to the container I, the crushing knife starts to crush and stir to achieve the crushing, mixing and extraction of the sample. After the crushing is completed, all / part of the extracted liquid enters the next link; The enrichment and purification part can realize the dilution, enrichment and purification of the extract. The dilution can be achieved by adding a diluent to the sealed container II. The diluted liquid passes through the capture area of ​​the analyte connected to the container II and then flows into the container IV for collecting waste liquid through the channel I. The diluted liquid flows out to the container IV after passing through the immunoaffinity column or immunomagnetic beads; by adding a washing liquid to the container II, it passes through the immunoaffinity column or immunomagnetic beads again and flows out to the container IV through the channel I, so as to wash away the impurities of the immunoaffinity column or immunomagnetic beads; after washing, an eluent is added to the immunoaffinity column or immunomagnetic beads, and the captured analyte is eluted and flows out to the container II through the channel II; The automated sample preparation pre-treatment device for aflatoxin detection can realize sample crushing, quantitative addition / exclusion of extract, quantitative addition / exclusion of diluent, quantitative addition / exclusion of eluent, quantitative addition / exclusion of eluent, collection of waste liquid, and collection of supernatant.

2. The automated sample preparation pre-treatment device for aflatoxin detection according to claim 1, characterized in that: The addition of extraction solution, diluent, eluent and elution solution is achieved by a liquid pump / air pump.

3. The automated sample preparation pre-treatment device for aflatoxin detection according to claim 1, characterized in that: The diluent and eluent should be the same liquid.

4. The automated sample preparation pre-treatment device for aflatoxin detection according to claim 1, characterized in that: The downward outlet of the immunoaffinity column or immunomagnetic beads is two channels. Channel I is a common channel for the diluent and the eluent to flow out after passing through the immunoaffinity column or immunomagnetic beads, and channel II is a collection channel for the eluent to flow out after passing through the immunoaffinity column or immunomagnetic beads.

5. The automated sample preparation pre-treatment device for aflatoxin detection according to claims 1-5, characterized in that: It includes a gas circuit part, a liquid circuit part, and a control part. The gas circuit part provides pressure for the liquid circuit part under the sequential control of the control part to complete the addition and discharge of various liquids. The gas circuit mainly includes an air filter, an air pump and multiple two-position two-way solenoid valves; the air pump is a positive pressure pump, which mainly provides positive pressure to each liquid storage device, extraction device and dilution device to realize gas-liquid conversion to achieve the effect of adding and discharging liquid; The two-position, two-way solenoid valve is in the normally closed position. When powered on, it can connect the gas pipeline, and when the solenoid valve is powered on, it can connect the corresponding flow pipeline.

6. The automated sample preparation pre-treatment device for aflatoxin detection according to claim 5, characterized in that: The liquid circuit part includes an extraction liquid storage unit, a diluent / rinsing liquid storage unit, an eluent storage unit, a container I, a container II, a target object capture area, a container IV and a container III; the extraction liquid storage unit 18, the container I, the container II, the diluent / rinsing liquid storage unit and the eluent storage unit are connected to the pipeline through a two-position two-way solenoid valve, and the gas source is used as the power source.

7. The automated sample preparation pre-treatment device for aflatoxin detection according to claim 6, characterized in that: The extracting liquid storage device and container I, container I and container II, and diluent / rinsing liquid storage device and container II are all connected through liquid pipelines. Check valves are also connected to the pipelines to prevent liquid backflow. A two-position, two-way solenoid valve is also arranged between container I and container II to connect and disconnect the circuit between the two containers.

8. The automated sample preparation pre-treatment device for aflatoxin detection according to claim 7, characterized in that: The target capture zone is connected to container II upstream, and has two outlets arranged downstream that are connected to container IV and container III respectively.

9. The automated sample preparation pre-treatment device for aflatoxin detection according to claim 8, characterized in that: The target capture area includes a two-position two-way solenoid valve, an immunoaffinity column, and a two-position three-way solenoid valve in sequence; the normal position of the two-position two-way solenoid valve is the left position which is normally off, and the right position is connected after power is supplied, and the upstream is connected to container II, and the downstream is connected to the inlet of the immunoaffinity column.

10. The automated sample preparation pre-treatment device for aflatoxin detection according to claim 9, characterized in that: Downstream of the immunoaffinity column is a two-position three-way solenoid valve which is normally connected to the left, and its downstream is connected to container IV; when energized, the two-position three-way solenoid valve changes to the right position, and its downstream is connected to container III; the two-position two-way solenoid valve and the immunoaffinity column are connected to the eluent storage via a liquid pipeline, and a check valve is connected to the liquid pipeline.

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