Experimental device for monitoring mouse epilepsy or sugar preference behaviors for long time

Abstract

The invention discloses an experiment device for monitoring mouse epilepsy or sugar preference behaviors for a long time, which comprises a box body and a bottom plate, a lifting mechanism is arranged on the bottom plate, and a testing mechanism is arranged on the lifting mechanism; the testing mechanism comprises a mouse electroencephalogram / myoelectricity monitoring device and a high-definition infrared camera; and a trough and a drinking bottle are arranged on the box body. Wherein the high-definition infrared camera is used for monitoring mouse behaviors, the mouse electroencephalogram / myoelectricity monitoring device is used for invasive monitoring, the lifting mechanism is used for adjusting the direction of the testing mechanism, and the feeding trough and the drinking water bottle are used for feeding food, drinking water or reagent and the like respectively. In conclusion, the device is reasonable in structure, convenient to use, economical, practical and multipurpose, can be used as a mouse feeding device, can also be used as an experiment observation device, and is particularly suitable for experiments for monitoring mouse epilepsy or sugar preference behaviors for a long time.

Description

Technical Field

[0001] The invention belongs to the technical field of mouse experimental devices, and in particular relates to a device for long-term monitoring of mouse epilepsy or sugar preference behavior experiments. Background Art

[0002] As social, environmental and psychological pressures increase, the risk of mental, cardiovascular and cerebrovascular diseases and neurological diseases in the population increases accordingly. Such people will experience epilepsy, depression, fatigue, indifference, cognitive impairment, etc., which will reduce the quality of life and even increase the burden on the family.

[0003] At present, the monitoring of mouse epilepsy behavior mainly involves invasive monitoring based on EEG / EMG with electrodes placed on the brain or muscles, or monitoring of mouse physiological activities based on pressure sensors. The former is an invasive operation and may itself become a factor leading to abnormal mouse behavior, while the latter requires high-cost hardware and software devices to assist in monitoring, which is less convenient and universal. In addition, many existing monitoring devices have single-purpose functions and are less economical. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a device for long-term monitoring of mouse epilepsy or sugar preference behavior experiments which has a reasonable structure, is easy to use and is economical and practical.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0006] A device for long-term monitoring of mouse epilepsy or sugar preference behavior experiments includes a box body and a bottom plate. The bottom plate is provided with a lifting mechanism, and a testing mechanism is provided on the lifting mechanism; the testing mechanism includes a mouse electroencephalogram / electromyography monitoring device and a high-definition infrared camera; and a food trough and a water bottle are provided on the box body.

[0007] The lifting mechanism includes four lifting rods arranged on both sides of the base plate, each lifting rod is provided with a retractable lifting bracket, sliding rods are connected between the top ends of the two lifting brackets on the left and between the top ends of the two lifting brackets on the right, a drilled damping guide rail is slidably connected between the left slide bar and the right slide bar, and the testing mechanism is installed on the drilled damping guide rail.

[0008] The two ends of the drilled damping rail are slidably arranged on the left and right slide bars through the slide bar sleeves.

[0009] The lifting rod and lifting bracket are adjusted by loose nuts.

[0010] A high-definition infrared camera is installed on the lower surface of the drilled damping guide rail, and a mouse electroencephalogram / electromyography monitoring device is installed on the upper surface of the drilled damping guide rail. A plurality of adjustment holes are arranged along the length direction of the drilled damping guide rail.

[0011] The mouse electroencephalogram / electromyogram monitoring device is connected to a high-definition infrared camera and controlled by a computer.

[0012] The feeding trough is arranged on the outer wall of the box body and communicates with the inside of the box body; the drinking bottle is arranged above the feeding trough and the drinking water pipe extends into the box body.

[0013] There are two drinking bottles, one is a sugar water drinking bottle and the other is a fresh water drinking bottle.

[0014] The box body is made of polycarbonate material, the bottom plate is made of alloy, and the feeding trough is made of metal.

[0015] The above device is used for long-term monitoring of mouse epilepsy or sugar preference behavior experiments.

[0016] In view of the problems existing in the current mouse behavior monitoring, the inventor designed a device for long-term monitoring of mouse epilepsy or sugar preference behavior experiments, including a box body, and also including a bottom plate. An elevating mechanism is arranged on the bottom plate, and a testing mechanism is arranged on the elevating mechanism; the testing mechanism includes a mouse electroencephalogram / electromyogram monitoring device and a high-definition infrared camera; a feeding trough and a drinking bottle are arranged on the box body. Among them, the high-definition infrared camera is used to monitor the behavior performance of the mouse, the mouse electroencephalogram / electromyogram monitoring device is used for invasive monitoring, the elevating mechanism is used to adjust the orientation of the testing mechanism, and the feeding trough and the drinking bottle are respectively used for feeding such as food, drinking water or test drugs. In summary, the device of the present invention has a reasonable structure, is convenient to use, economical and practical, and has multiple uses. It can be used as a mouse breeding device and also as an experimental observation device, especially suitable for long-term monitoring of mouse epilepsy or sugar preference behavior experiments. Description of the Drawings

[0017] Figure 1 It is a schematic structural diagram of the device for long-term monitoring of mouse epilepsy or sugar preference behavior experiments of the present invention.

[0018] In the figure: 1 bottom plate, 2 box body, 3 feeding trough, 4 drinking bottle, 5 lifting rod, 6 tightening nut, 7 lifting bracket, 8 sliding rod sleeve, 9 mouse electroencephalogram / electromyogram monitoring device, 10 adjusting hole, 11 high-definition infrared camera, 12 computer, 13 drilling damping guide rail, 14 left sliding rod, 15 right sliding rod. Detailed Embodiments

[0019] I. Basic Structure

[0020] As Figure 1 shown, the device for long-term monitoring of mouse epilepsy or sugar preference behavior experiments of the present invention includes a box body 2 made of polycarbonate material, which is the living environment of the mouse; it also includes a bottom plate 1 made of alloy, which provides a stable installation platform for the whole device. An elevating mechanism is arranged on the bottom plate, and a testing mechanism is arranged on the elevating mechanism; the testing mechanism includes a mouse electroencephalogram / electromyogram monitoring device (such as Small Animal Electroencephalogram and Electromyogram (EEG&EMG) Research System, Shanghai Ran Zhe Instrument Equipment Co., Ltd.) and a high-definition infrared camera; a water bottle 4 and a metal food trough 3 are provided on the box body. Among them,

[0021] The lifting mechanism includes four lifting rods 5 arranged on both sides of the bottom plate. Each lifting rod is provided with a telescopic lifting bracket 7. The lifting rod and the lifting bracket are adjusted for lifting through a tension nut 6. A sliding rod is connected between the tops of the two left lifting brackets and between the tops of the two right lifting brackets. A drilling damping guide rail is slidably connected between the left sliding rod 14 and the right sliding rod 15. Both ends of the drilling damping guide rail are arranged on the left and right sliding rods through sliding rod sleeves. The position of the drilling damping guide rail on the sliding rod can be adjusted back and forth through the sliding rod sleeves. The testing mechanism is installed on the 13 drilling damping guide rails.

[0022] The high-definition infrared camera 11 is installed on the lower surface of the drilling damping guide rail and can be moved left and right along the drilling damping guide rail to adjust the position. The mouse electroencephalogram / electromyogram monitoring device 9 is installed on the upper surface of the drilling damping guide rail. A plurality of adjustment holes 10 are arranged along the length direction of the drilling damping guide rail to facilitate the wiring of the device. The mouse electroencephalogram / electromyogram monitoring device is connected to the high-definition infrared camera and is controlled by a computer 12.

[0023] The food trough is arranged on the outer wall of the box body and communicates with the inside of the box body; the water bottle is arranged above the food trough and the water pipe extends into the box body. The water bottle includes two, one is a sugar water drinking bottle and the other is a fresh water drinking bottle.

[0024] II. Usage method

[0025] (1) The box body can be specifically used for raising mice, which is convenient for mice to adapt to the environment in advance and saves the environmental adaptation link after transferring the mice from the breeding area to the observation area.

[0026] (2) Conduct epilepsy behavior monitoring and analysis experiments. First, put the model mice into the box body for breeding. When the experiment is needed, put the mice together with the box body on the bottom plate for experimental observation. The lifting rod can adjust the height of the camera up and down, the drilling damping guide rail can adjust the camera back and forth along the left and right sliding rods, and the camera can move left and right along the drilling damping guide rail, which is convenient for the experimenter to find a suitable monitoring field of view. The behavior of the mice can be clearly monitored for 24 hours through the high-definition infrared camera, and the monitoring data is transmitted back to the computer for storage. Then, view the playback on the computer to judge whether there is epilepsy behavior. When the behavior is not obvious, or the experimenter wants to further verify whether the mice have epilepsy, the mouse electroencephalogram / electromyogram monitoring device can be used for monitoring. The electroencephalogram and electromyogram are transmitted back to the computer and analyzed with corresponding professional software to avoid interfering with the behavior of the mice by directly using invasive operations.

[0027] (3) Conduct a sucrose preference experiment. Two drinking bottles are respectively filled with sucrose solution and fresh water. Observe the choices of depressive mice between the sucrose solution and fresh water. The positions of the drinking bottles can be swapped regularly to prevent the mice from remembering their drinking habits. If only epileptic behavior needs to be monitored, the extra drinking bottles can be removed.

Claims

1. A device for long-term monitoring of mouse epilepsy or sugar preference behavior experiments, comprising a box, characterized in that It also includes a bottom plate, on which a lifting mechanism is arranged, and on which a testing mechanism is arranged; the testing mechanism includes a mouse electroencephalogram / electromyography monitoring device and a high-definition infrared camera; and the box body is provided with a food trough and a water bottle.

2. The device according to claim 1, characterized in that: The lifting mechanism includes four lifting rods arranged on both sides of the base plate, each lifting rod is provided with a retractable lifting bracket, a sliding rod is connected between the top ends of the two lifting brackets on the left and between the top ends of the two lifting brackets on the right, a drilled damping guide rail is slidably connected between the left slide rod and the right slide rod, and the testing mechanism is installed on the drilled damping guide rail.

3. The device according to claim 1, characterized in that: The two ends of the drilling damping guide rail are slidably arranged on the left sliding rod and the right sliding rod through sliding rod sleeves.

4. The device according to claim 2, characterized in that: The lifting rod and the lifting bracket are adjusted by loose nuts.

5. The device according to claim 1, characterized in that: The high-definition infrared camera is installed on the lower surface of the drilled damping guide rail, and the mouse electroencephalogram / electromyography monitoring device is installed on the upper surface of the drilled damping guide rail. A plurality of adjustment holes are arranged along the length direction of the drilled damping guide rail.

6. The device according to claim 5, characterized in that: The mouse EEG / EMG monitoring device is connected to a high-definition infrared camera and controlled by a computer.

7. The device according to claim 1, characterized in that: The food trough is arranged on the outer wall of the box body and is connected to the box body; The drinking water bottle is arranged above the food trough and the drinking water pipe extends into the box body.

8. The device according to claim 6, characterized in that: The drinking water bottles include two, one is a sugar water drinking water bottle, and the other is a fresh water drinking water bottle.

9. The device according to claim 1, characterized in that: The box body is made of polycarbonate material, the bottom plate is made of alloy, and the feeding trough is made of metal.

10. The device of claim 1 is used for long-term monitoring of epilepsy or sugar preference behavior experiments in mice.

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