Exosome separation and extraction device and method

Abstract

The invention relates to the technical field of exosome separation and extraction, and discloses an exosome separation and extraction device and method, and the exosome separation and extraction device comprises: a barrel-shaped separation cabin; the cover plate can cover the separating chamber; the filter cartridge is separably mounted in the separation cabin, the filter cartridge is barrel-shaped and is provided with a cavity, and filter holes are formed in the inner wall of the cavity; the driving assembly is mounted in the separation cabin and is in transmission connection with the filter cartridge; the flow guide assembly is connected to the bottom of the separation cabin, the flow guide assembly is provided with a flow guide flow channel, the flow guide flow channel is communicated with the containing cavity of the separation cabin, and the flow guide assembly can guide the exosome separation liquid in the separation cabin out of the exosome separation and extraction device through the flow guide flow channel. According to the exosome separation device, the filter cartridge is separably mounted in the accommodating cavity of the separation cabin, and the filter cartridge is taken out after separation and extraction, so that the interiors of the separation cabin and the filter cartridge can be thoroughly cleaned, and the situation that subsequent exosome research is interfered by residual substances in the device is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of exosome separation and extraction, and particularly relates to an exosome separation and extraction device and a separation and extraction method. Background Art

[0002] Exosomes refer to small membrane vesicles containing complex RNA and proteins, and now specifically refer to discoid vesicles with a diameter of 40-100 nm. A variety of cells can secrete exosomes under normal and pathological conditions. They mainly originate from multi-vesicular bodies formed by the invagination of lysosome particles within cells, and are released into the extracellular matrix after the outer membrane of the multi-vesicular body fuses with the cell membrane.

[0003] When obtaining exosomes for research and examination, the difficulty lies in how to efficiently separate and purify exosomes. In the prior art, exosomes are usually separated and purified by separation and extraction equipment. However, existing equipment is usually integrated, having the problems of being inconvenient to disassemble and clean. And equipment that is not thoroughly cleaned will interfere with subsequent research due to the residues in the equipment during subsequent use, causing deviations in the research. Moreover, if the equipment is not thoroughly cleaned for a long time, it will also affect the performance and accuracy of the separation and extraction equipment, affecting the separation effect and subsequent research results. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention provides an exosome separation and extraction device and a separation and extraction method.

[0005] In a first aspect, the present invention provides an exosome separation and extraction device, including: a separation chamber, the separation chamber is barrel-shaped and has a containing cavity; a cover plate, the cover plate can cover the separation chamber; a filter cartridge, the filter cartridge is detachably installed in the containing cavity of the separation chamber, the filter cartridge is barrel-shaped and has a cavity for containing exosomes, and filter holes communicating with the containing cavity of the separation chamber are provided on the inner wall of the cavity; a driving component, the driving component is installed on the separation chamber and is in transmission connection with the filter cartridge; the driving component can drive the filter cartridge to rotate to centrifuge the exosomes in the cavity of the filter cartridge; a diversion component, the diversion component is connected to the bottom of the separation chamber, the diversion component has a diversion channel, the diversion channel communicates with the containing cavity of the separation chamber, and the diversion component can export the exosome separation liquid in the separation chamber out of the exosome separation and extraction device through the diversion channel.

[0006] Optionally, a vibration component is installed on the inner wall of the separation chamber. The vibration component includes an impact block that can telescopically extend along the radial direction of the separation chamber, and an energy storage part that can drive the impact block to impact the filter cartridge; the impact block contacts the outer side wall of the filter cartridge, and a plurality of grooves are provided on the outer side wall of the filter cartridge; when the filter cartridge rotates, the grooves move with the filter cartridge, and when the impact block faces the groove, the energy storage part drives the impact block so that the impact block extends into the groove to strike the filter cartridge.

[0007] Optionally, the vibration assembly further includes a telescopic member. The telescopic member is installed on the inner wall of the separation chamber and includes a telescopic rod that can telescopically extend along the radial direction of the separation chamber. The energy storage part is a spring, and the spring is sleeved on the telescopic member. One end of the spring is connected to the inner wall of the separation chamber, and the other end is connected to the telescopic rod. The impact block is connected to the telescopic rod. The outer side wall of the filter cartridge includes a position where a groove is opened and a position where no groove is opened. When the impact block contacts the position where no groove is opened, the spring is in a compressed state. When the impact block faces the position where the groove is opened, the spring drives the telescopic rod to extend, and the filter cartridge is struck by the impact block.

[0008] Optionally, the driving assembly includes a driving member and an output member. The driving member and the output member are in transmission connection, and the driving member can drive the output member to rotate. The output member is rotatably installed in the accommodating cavity of the separation chamber, dividing the accommodating cavity into upper and lower parts. The output member is provided with an outflow port through which the exosome separation liquid can flow to the bottom of the accommodating cavity of the separation chamber. The upper surface of the output member has a protruding portion, and a positioning groove is opened on the bottom end surface of the filter cartridge. When the filter cartridge is installed in the separation chamber, the protruding portion on the output member is inserted into the positioning groove of the filter cartridge. When the driving member drives the output member to rotate, the output member drives the filter cartridge to rotate through the cooperation of the protruding portion and the positioning groove.

[0009] Optionally, the driving member includes a motor installed outside the separation chamber and a gear coaxially arranged with the output shaft of the motor. The output member has a circular plate-like structure, and the outer edge of the output member is provided with meshing teeth that can mesh with the gear. An opening is provided on the side wall of the separation chamber, and the gear extends into the separation chamber through the opening and meshes with the output member.

[0010] Optionally, trumpet-shaped flow guiding members are rotatably installed on both the upper surface and the lower surface of the output member, and both flow guiding members are fixed to the inner wall of the separation chamber.

[0011] Optionally, the top of the filter cartridge is turned outwards to form an annular limiting portion. A first limiting plate is provided at the upper part of the inner wall of the separation chamber. When the filter cartridge is detachably installed in the separation chamber, the first limiting plate supports the limiting portion of the filter cartridge.

[0012] Optionally, a sealing gasket and a plurality of limiting rods are installed on the cover plate. An annular second limiting plate is sleeved on the outer circumference of the top of the separation chamber, and a plurality of limiting holes are opened on the second limiting plate. When the cover plate covers the separation chamber, the limiting rods are inserted into the corresponding limiting holes one by one, and the sealing gasket is located between the cover plate and the filter cartridge to cover the filter cartridge.

[0013] Optionally, the flow guiding assembly includes a flow guiding pipe located outside the separation chamber. One end of the flow guiding pipe is connected to the bottom of the separation chamber and communicates with the accommodating cavity of the separation chamber, and the other end of the flow guiding pipe is an outlet end. A control valve is provided on the flow guiding pipe.

[0014] In a second aspect, the present invention provides a separation and extraction method for an exosome separation and extraction device, including:

[0015] Open the cover plate and place the pre-acquired exosomes into the filter cartridge cavity;

[0016] Close the separation chamber cover through the cover plate;

[0017] Start the drive assembly to perform centrifugal separation on the exosomes in the filter cartridge;

[0018] After centrifugal separation, open the cover plate and take out the filter cartridge to obtain the exosome separation liquid A in the filter cartridge; through the diversion flow channel of the diversion assembly, obtain the exosome separation liquid B after centrifugal separation;

[0019] After extracting the exosome separation liquid A and the exosome separation liquid B, clean the exosome separation and extraction device.

[0020] The technical solution provided by the embodiment of the present invention has the following advantages compared with the prior art:

[0021] 1. By detachably installing the filter cartridge in the accommodating cavity of the separation chamber, after the exosomes are separated and extracted, by taking out the filter cartridge, the inside of the separation chamber and the filter cartridge can be thoroughly cleaned, avoiding interference with subsequent exosome research due to residual substances in the device. At the same time, since the filter cartridge can be taken out of the separation chamber, the staff can conveniently clean the filter cartridge and the inside of the separation chamber, reducing the cleaning difficulty of the staff.

[0022] 2. By setting the drive assembly, the filter cartridge can be driven to rotate; therefore, when the filter cartridge rotates, the exosomes in the filter cartridge are separated under the action of centrifugal force. Therefore, the centrifugal extraction device of the present invention only needs to set the drive assembly and the filter cartridge to complete centrifugal separation, and has the advantages of simple structure and convenient operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic structural diagram of an exosome separation and extraction device provided by an embodiment of the present invention.

[0024] Figure 2 It is a schematic structural diagram of a vibration assembly arranged in the separation chamber provided by an embodiment of the present invention.

[0025] Figure 3 It is a schematic structural diagram of a filter cartridge arranged in the separation chamber provided by an embodiment of the present invention.

[0026] Figure 4 is Figure 3 the enlarged view at A in

[0027] Figure 5 It is a schematic structural diagram of the drive assembly provided by an embodiment of the present invention.

[0028] Figure 6 It is a schematic structural diagram of the filter cartridge and the drive assembly provided by an embodiment of the present invention.

[0029] Figure 7 This is a schematic structural diagram of the driving member and the output member provided by the embodiment of the present invention.

[0030] Figure 8 This is a schematic structural diagram of a diversion member provided on the output member provided by the embodiment of the present invention.

[0031] Figure 9 This is a schematic structural diagram of the cover plate and the separation chamber provided by the embodiment of the present invention.

[0032] Explanation of reference numerals: 1, separation chamber; 111, diversion pipe; 112, control valve; 113, first limit plate; 114, second limit plate; 2, filter cartridge; 211, cover plate; 212, handle; 213, limit portion; 214, pull ring; 215, sealing gasket; 216, limit rod; 217, limit hole; 3, drive assembly; 311, support frame; 312, motor; 313, diversion member; 314, gear; 315, rotating shaft; 316, output member; 317, protruding portion; 318, positioning groove; 4, vibration assembly; 411, telescopic member; 412, telescopic rod; 413, impact block; 414, spring. Detailed Description of the Invention

[0033] The following will describe in detail a specific embodiment of the present invention with reference to the accompanying drawings. However, it should be understood that the protection scope of the present invention is not limited by the specific embodiment.

[0034] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the technical solutions of the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0035] Embodiment 1:

[0036] As Figure 1 and Figure 3 shown, the embodiment of the present invention provides an exosome separation and extraction device, including:

[0037] A separation chamber 1, the separation chamber 1 is in a barrel shape and has an accommodation cavity.

[0038] The bottom of the separation chamber 1 is provided with at least three legs, and all the legs are circumferentially and evenly distributed around the outer wall of the separation chamber 1; in this embodiment, there are four legs for supporting the separation chamber 1.

[0039] A cover plate 211 which can cover the separation chamber 1.

[0040] In this embodiment, a handle 212 is installed on the upper surface of the cover plate 211, and a ring-shaped protective eaves is installed on the lower surface of the cover plate 211. When the cover plate 211 covers the separation chamber 1, the protective eaves is located outside the limiting plate 113.

[0041] A filter cartridge 2 which is detachably installed in the accommodation cavity of the separation chamber 1. The filter cartridge 2 is in a barrel shape and has a cavity for containing exosomes. Filter holes communicating with the accommodation cavity of the separation chamber 1 are provided on the inner wall of the cavity.

[0042] In this embodiment, filter holes are provided on the filter cartridge 2, and two pull rings 214 are provided at the top of the filter cartridge 2, located on opposite sides at the top of the filter cartridge.

[0043] In this embodiment, there are multiple protrusions on the inner wall of the filter cartridge, which can increase the contact area between the exosomes and the inner wall of the filter cartridge 2, making it easier for the exosomes to enter the separation chamber 1 through the filter holes under the action of centrifugal force, and further improving the centrifugal separation efficiency.

[0044] A driving assembly 3 which is installed on the separation chamber 1 and is in transmission connection with the filter cartridge 2; the driving assembly 3 can drive the filter cartridge 2 to rotate to centrifuge the exosomes in the cavity of the filter cartridge 2.

[0045] A diversion assembly which is connected to the bottom of the separation chamber 1. The diversion assembly has a diversion flow channel which communicates with the accommodation cavity of the separation chamber 1. The diversion assembly can export the exosome separation liquid in the separation chamber 1 out of the exosome separation and extraction device through the diversion flow channel.

[0046] In this embodiment, a flow splitting plate is provided at a position on the inner wall of the separation chamber 1 close to the inlet of the diversion flow channel. Multiple flow splitting holes are provided on the flow splitting plate, and the flow splitting plate can split the exosome separation liquid into the diversion flow channel.

[0047] Such as Figure 2 、 Figure 3 、 Figure 5 、 Figure 6 And Figure 7As shown, a vibration assembly 4 is installed on the inner wall of the separation chamber 1. The vibration assembly 4 includes an impact block 413 that can telescopically extend and retract in the radial direction of the separation chamber 1, and an energy storage part that can drive the impact block 413 to impact the filter cartridge 2. The impact block 413 contacts the outer side wall of the filter cartridge 2, and a plurality of grooves are formed on the outer side wall of the filter cartridge 2. When the filter cartridge 2 rotates, the grooves move with the filter cartridge 2. When the impact block 413 faces the grooves, the energy storage part drives the impact block 413 so that the impact block 413 extends into the grooves to strike the filter cartridge 2.

[0048] In this embodiment, the number of the vibration assemblies 4 is multiple, and they are evenly arranged on the inner wall of the separation chamber 1.

[0049] As Figure 2 shown, the vibration assembly 4 further includes a telescopic member 411. The telescopic member 411 is installed on the inner wall of the separation chamber 1 and includes a telescopic rod 412 that can telescopically extend and retract in the radial direction of the separation chamber 1. The energy storage part is a spring 414. The spring 414 is sleeved on the telescopic member 411. One end of the spring 414 is connected to the inner wall of the separation chamber 1, and the other end is connected to the telescopic rod 412. The impact block 413 is connected to the telescopic rod 412. The outer side wall of the filter cartridge 2 includes a position where the grooves are formed and a position where no grooves are formed. When the impact block 413 contacts the position where no grooves are formed, the spring 414 is in a compressed state. When the impact block 413 faces the position where the grooves are formed, the spring 414 drives the telescopic rod 412 to extend, and the filter cartridge 2 is struck by the impact block 413.

[0050] In this embodiment, the telescopic member 411 has multiple telescopic joints, and adjacent two telescopic joints can telescopically extend and retract. The innermost telescopic joint is the above-mentioned telescopic rod 412.

[0051] As Figure 5 、 Figure 6 、 Figure 7 and Figure 8 shown, the driving assembly 3 includes a driving member and an output member 316. The driving member is in transmission connection with the output member 316, and the driving member can drive the output member 316 to rotate. The output member 316 is rotatably installed in the accommodating cavity of the separation chamber 1, dividing the accommodating cavity into upper and lower parts. The output member 316 is provided with a flow outlet that can make the exosome separation liquid flow to the bottom of the accommodating cavity of the separation chamber 1. The upper surface of the output member 316 has a protruding portion 317. A positioning groove 318 is formed on the bottom end surface of the filter cartridge 2. When the filter cartridge 2 is installed in the separation chamber 1, the protruding portion 317 on the output member 316 is inserted into the positioning groove 318 of the filter cartridge 2. When the driving member drives the output member 316 to rotate, the output member 316 drives the filter cartridge 2 to rotate through the cooperation of the protruding portion 317 and the positioning groove 318.

[0052] In this embodiment, the exosome separation liquid B enters the accommodating cavity of the separation chamber 1 through the filter holes on the filter cartridge 2, and then flows to the bottom of the accommodating cavity through the outflow port on the output member 316; the exosome separation liquid A remains in the filter cartridge 2 and is directly taken out from the filter cartridge after separation.

[0053] In this embodiment, the output member 316 divides the accommodating cavity into upper and lower parts. The outflow ports on the output member 316 can communicate the upper and lower parts of the accommodating cavity, and the number of the outflow ports is multiple.

[0054] In this embodiment, both the protruding portion 317 and the positioning groove 318 are prismatic, so that the protruding portion can drive the filter cartridge 2 to rotate when it rotates.

[0055] As Figure 3 shown, the driving member includes a motor 312 installed outside the separation chamber 1 and a gear 314 coaxially arranged with the output shaft of the motor 312; the output member 316 is a circular plate-like structure, and engaging teeth capable of meshing with the gear 314 are provided on the outer edge of the output member 316; an opening is provided on the side wall of the separation chamber 1, and the gear 314 extends into the separation chamber 1 through the opening and meshes with the output member 316.

[0056] As Figure 4 shown, in this embodiment, a support frame 311 is provided outside the separation chamber 1, the motor is installed on the support frame 311, a rotating shaft 315 connected to the output shaft of the motor is provided inside the support frame 311, the output shaft of the motor and the rotating shaft 315 are connected by a coupling, the gear 315 is installed on the rotating shaft 315, the gear and the output shaft of the motor are coaxially arranged through the rotating shaft, and the motor 312 is a variable-frequency motor, which can accurately adjust the rotation speed according to different separation requirements, further improving the accuracy of centrifugal separation.

[0057] Flared guiding members 313 are rotatably installed on both the upper surface and the lower surface of the output member 316, and both guiding members 313 are fixed to the inner wall of the separation chamber 1.

[0058] In this embodiment, the guiding member 313 is annular; annular sliding grooves are provided on both the upper surface and the lower surface of the output member 316, and the lower end of the upper guiding member 313 and the upper end of the lower guiding member 313 are respectively slidably arranged in the corresponding sliding grooves. The lower guiding member has the function of supporting the output member 316. In other embodiments, the rotational connection can be realized through bearings.

[0059] The top of the filter cartridge 2 is turned outwards to form an annular limiting portion 213; a first limiting plate 113 is provided at the upper part of the inner wall of the separation chamber 1. When the filter cartridge 2 is detachably installed in the separation chamber 1, the first limiting plate 113 supports the limiting portion 213 of the filter cartridge 2.

[0060] As Figure 9As shown in the figure, a gasket 215 and a plurality of limiting rods 216 are installed on the cover plate 211; a ring-shaped second limiting plate 114 is sleeved on the outer circumference of the top of the separation chamber 1, and a plurality of limiting holes 217 are opened on the second limiting plate 114; when the cover plate 211 covers the separation chamber 1, the limiting rods 216 are inserted into the corresponding limiting holes 217 one by one, and the gasket 215 is located between the cover plate 211 and the filter cartridge 2 to cover the filter cartridge 2.

[0061] In this embodiment, the plurality of limiting rods 216 are circumferentially and uniformly arranged around the central axis of the cover plate 211; the number of the limiting holes is the same as that of the limiting rods, and the plurality of limiting holes are circumferentially and uniformly distributed around the central axis of the first limiting plate 115.

[0062] The diversion assembly includes a diversion pipe 111 located outside the separation chamber 1; one end of the diversion pipe 111 is connected to the bottom of the separation chamber 1 and communicates with the accommodation cavity of the separation chamber 1, and the other end of the diversion pipe 111 is an outlet end; a control valve 112 is provided on the diversion pipe 111.

[0063] In this embodiment, it further includes a controller, as well as a temperature sensor and a pressure sensor electrically connected to the controller. The temperature sensor and the pressure sensor are both installed on the inner wall of the separation chamber 1, and the temperature sensor and the pressure sensor monitor the temperature and pressure inside the separation chamber 1 in real time; when the monitored temperature or pressure is greater than the preset value, the controller controls the motor to reduce the motor speed, so as to ensure the stability of the separation process.

[0064] Embodiment 2:

[0065] Based on the exosome separation and extraction device of Embodiment 1, this embodiment provides a separation and extraction method for an exosome separation and extraction device, including:

[0066] Step 1: Open the cover plate 211 and place the pre-acquired exosomes in the cavity of the filter cartridge 2;

[0067] Step 2: Cover the separation chamber 1 through the cover plate 211;

[0068] Step 3: Start the driving assembly 3 to perform centrifugal separation on the exosomes in the filter cartridge 2;

[0069] In this embodiment, the rotation speed of the filter cartridge 2 is controlled according to the preset separation conditions, so that the exosomes are efficiently separated under the action of centrifugal force;

[0070] Step 4: After centrifugal separation, open the cover plate 211 to take out the filter cartridge 2 to obtain the exosome separation liquid A in the filter cartridge 2; through the diversion channel of the diversion assembly, obtain the exosome separation liquid B after centrifugal separation;

[0071] Step 5: After extracting the exosome separation liquid A and the exosome separation liquid B, clean the exosome separation and extraction device.

[0072] In this embodiment, the exosome separation and extraction device needs to be cleaned in advance before separation and extraction.

[0073] The above are only several specific embodiments of the present invention. However, the embodiments of the present invention are not limited thereto, and any changes that can be conceived by those skilled in the art should fall within the protection scope of the present invention.

Claims

1. An exosome isolation and extraction device, characterized in that, Comprising: A separation chamber (1), the separation chamber (1) being barrel-shaped and having a receiving cavity; A cover plate (211), the cover plate (211) being capable of covering the separation chamber (1); A filter cartridge (2), the filter cartridge (2) being detachably installed in the receiving cavity of the separation chamber (1), the filter cartridge (2) being barrel-shaped and having a cavity for containing exosomes, and filter holes communicating with the receiving cavity of the separation chamber (1) being provided on the inner wall of the cavity; A driving assembly (3), the driving assembly (3) being installed on the separation chamber (1) and being in transmission connection with the filter cartridge (2); the driving assembly (3) being capable of driving the filter cartridge (2) to rotate and centrifugally separating the exosomes in the cavity of the filter cartridge (2); A diversion assembly, the diversion assembly being connected to the bottom of the separation chamber (1), the diversion assembly having a diversion flow channel, the diversion flow channel communicating with the receiving cavity of the separation chamber (1), and the diversion assembly being capable of discharging the exosome separation liquid in the separation chamber (1) out of the exosome separation and extraction device through the diversion flow channel.

2. The exosome separation and extraction device according to claim 1, wherein, A vibration assembly (4) is installed on the inner wall of the separation chamber (1), the vibration assembly (4) including an impact block (413) capable of radially expanding and contracting along the separation chamber (1), and an energy storage part capable of driving the impact block (413) to impact the filter cartridge (2); The impact block (413) contacts the outer side wall of the filter cartridge (2), and a plurality of grooves are provided on the outer side wall of the filter cartridge (2); when the filter cartridge (2) rotates, the grooves move with the filter cartridge (2), and when the impact block (413) faces the grooves, the energy storage part drives the impact block (413) so that the impact block (413) extends into the grooves to strike the filter cartridge (2).

3. The exosome separation and extraction device according to claim 2, characterized in that, The vibration assembly (4) further includes a telescopic member (411), the telescopic member (411) being installed on the inner wall of the separation chamber (1) and including a telescopic rod (412) capable of radially expanding and contracting along the separation chamber (1); The energy storage part is a spring (414), the spring (414) being sleeved on the telescopic member (411), one end of the spring (414) being connected to the inner wall of the separation chamber (1), the other end being connected to the telescopic rod (412), and the impact block (413) being connected to the telescopic rod (412); The outer side wall of the filter cartridge (2) includes a position where grooves are provided and a position where no grooves are provided. When the impact block (413) contacts the position where no grooves are provided, the spring (414) is in a compressed state. When the impact block (413) faces the position where grooves are provided, the spring (414) drives the telescopic rod (412) to extend, and the filter cartridge (2) is struck by the impact block (413).

4. The exosome separation and extraction device according to claim 1, wherein, The driving assembly (3) includes a driving member and an output member (316), the driving member and the output member (316) being in transmission connection, and the driving member being capable of driving the output member (316) to rotate; The output member (316) is rotatably installed in the receiving cavity of the separation chamber (1), dividing the receiving cavity into upper and lower parts, and a flow outlet through which the exosome separation liquid can flow to the bottom of the receiving cavity of the separation chamber (1) is provided on the output member (316); The upper surface of the output member (316) has a protruding portion (317), and a positioning groove (318) is formed on the bottom end surface of the filter cartridge (2). When the filter cartridge (2) is installed in the separation chamber (1), the protruding portion (317) on the output member (316) is inserted into the positioning groove (318) of the filter cartridge (2); when the driving member drives the output member (316) to rotate, the output member (316) drives the filter cartridge (2) to rotate through the cooperation of the protruding portion (317) and the positioning groove (318).

5. The exosome separation and extraction device according to claim 4, characterized in that, The driving member includes a motor (312) installed outside the separation chamber (1), and a gear (314) coaxially arranged with the output shaft of the motor (312); The output member (316) is of a circular plate-like structure, and meshing teeth capable of meshing with the gear (314) are formed on the outer edge of the output member (316); an opening is provided on the side wall of the separation chamber (1), and the gear (314) extends into the separation chamber (1) through the opening and meshes with the output member (316).

6. The exosome separation and extraction device according to claim 4, wherein, Fluid guiding members (313) in the shape of a flared opening are rotatably installed on both the upper surface and the lower surface of the output member (316), and both fluid guiding members (313) are fixed to the inner wall of the separation chamber (1).

7. The exosome separation and extraction device according to claim 1, characterized in that, The top of the filter cartridge (2) is turned outwards to form an annular limiting portion (213); a first limiting plate (113) is provided at the upper part of the inner wall of the separation chamber (1). When the filter cartridge (2) is detachably installed in the separation chamber (1), the first limiting plate (113) supports the limiting portion (213) of the filter cartridge (2).

8. The exosome separation and extraction device according to claim 1, characterized in that, A sealing gasket (215) and a plurality of limiting rods (216) are installed on the cover plate (211); an annular second limiting plate (114) is sleeved on the circumference of the top of the separation chamber (1), and a plurality of limiting holes (217) are formed in the second limiting plate (114); When the cover plate (211) closes the separation chamber (1), the limiting rods (216) are inserted into the corresponding limiting holes (217) one by one, and the sealing gasket (215) is located between the cover plate (211) and the filter cartridge (2) to close the filter cartridge (2).

9. The exosome separation and extraction device according to claim 1, wherein The fluid guiding assembly includes a fluid guiding pipe (111) located outside the separation chamber (1); one end of the fluid guiding pipe (111) is connected to the bottom of the separation chamber (1) and communicates with the accommodating cavity of the separation chamber (1), and the other end of the fluid guiding pipe (111) is the outlet end; a control valve (112) is provided on the fluid guiding pipe (111); a spiral fluid guiding groove is formed on the inner wall of the fluid guiding pipe (111).

10. The separation and extraction method of the exosome separation and extraction device according to any one of claims 1 to 9, characterized in that Including: Open the cover plate (211) and place the pre-acquired exosomes into the cavity of the filter cartridge (2); Cover the separation chamber (1) through the cover plate (211); Start the driving assembly (3) to perform centrifugal separation on the exosomes in the filter cartridge (2); After centrifugal separation, open the cover plate (211) and take out the filter cartridge (2) to obtain the exosome separation liquid A in the filter cartridge (2); through the fluid guiding channel of the fluid guiding assembly, obtain the exosome separation liquid B after centrifugal separation; After extracting the exosome separation liquid A and the exosome separation liquid B, clean the exosome separation and extraction device.

Images