Multifunctional indoor aerosol test system

Abstract

The invention discloses a multifunctional aerosol test system. Comprising a rotary drum reaction chamber; the aerosol generation subsystem is used for inputting aerosol to the test subsystem; the airflow regulation and control subsystem comprises an inlet side regulation and control module and an outlet side regulation and control module; the test substance release subsystem comprises disinfection liquid release equipment, volatile organic compound generation equipment and oxidant release equipment and is used for inputting a test substance to the test subsystem, and the test substance comprises one or more of disinfection liquid, a volatile organic compound and an oxidant; the detecting and monitoring subsystem is used for detecting and monitoring changes in the testing subsystem in real time and comprises an online measuring instrument, an aerosol sampling instrument and a particle size testing device. The test system disclosed by the invention can be used for realizing various aerosol tests such as indoor pathogen killing tests of buildings, indoor secondary organic aerosol synthesis tests and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of test systems, and particularly to an indoor aerosol test system. Background Art

[0002] According to statistics, more than 90% of urban residents spend their time in the indoor environment. Indoor air quality is affected by various sources such as indoor paint, dust, bacteria, and viruses, which greatly impacts people's quality of life, health level, and work efficiency. On the one hand, microorganisms such as indoor bacteria, fungi, and viruses have strong infectivity and sensitization, and may cause infectious diseases such as asthma, allergic dermatitis, and influenza A, and in severe cases, may lead to death. On the other hand, volatile organic compounds such as radon and formaldehyde are generated on the surface of indoor building materials. When irradiated by sunlight (mainly the ultraviolet part), they undergo a series of chemical reactions with oxidants such as ozone and OH radicals in the atmosphere, resulting in the formation of semi-volatile and low-volatile compounds, which then react with indoor particulate matter through gas-phase reactions, heterogeneous reactions, or liquid-phase reactions to ultimately form secondary organic aerosols that endanger human health.

[0003] The pollution of the indoor environment and the spread of microbial aerosols have attracted the attention of all sectors, and the demand for indoor air disinfection has increased. The methods of indoor air sterilization and disinfection can be divided into physical disinfection methods, biological disinfection methods, chemical disinfection methods, etc. A large number of studies have been conducted on various disinfection methods, but the improvement of the sterilization rate often focuses on the optimization of materials or system structures under test conditions, and it is difficult to reflect the actual disinfection rate of different sterilization methods on real indoor microbial aerosols.

[0004] The research methods for indoor secondary organic aerosol synthesis mainly rely on laboratory simulations. The smog chamber test is the earliest method used to study the reaction mechanism of VOCs. In the smog chamber test, VOCs, aerosol seeds, ozone (used to generate free radicals), inorganic gaseous precursors, and water vapor are introduced into the smog chamber, and the reaction conditions are controlled for monitoring and detecting the generated components and mass of secondary organic aerosols. However, the wall losses in the smog chamber make it difficult to study the aging process of secondary organic aerosols and also difficult to reflect the formation process of secondary organic aerosols under actual real atmospheric conditions. Summary of the Invention

[0005] In view of the above deficiencies in the prior art, the present invention provides a multifunctional indoor aerosol test system.

[0006] The technical solution adopted by the present invention is as follows:

[0007] I. A multifunctional indoor aerosol test system

[0008] The indoor aerosol test system includes:

[0009] A test subsystem, including a drum reaction chamber;

[0010] An aerosol generation subsystem for inputting aerosol into the test subsystem;

[0011] An air flow regulation subsystem, including an inlet side regulation module and an outlet side regulation module, which are respectively arranged on the inlet side and the outlet side of the drum reaction chamber;

[0012] A test substance release subsystem, including a disinfection liquid release device, a volatile organic compound generation device, and an oxidant release device, for inputting test substances into the test subsystem, and the test substances include one or more of disinfection liquid, volatile organic compound, and oxidant;

[0013] A detection and monitoring subsystem for real-time detecting and monitoring the changes of relevant parameters in the test subsystem, including an on-line measuring instrument, an aerosol sampling instrument, and a particle size testing device.

[0014] The indoor aerosol test system further includes a first three-way valve, a third three-way valve, a first four-way valve, and a second four-way valve;

[0015] The outlets of the volatile organic compound generation device and the oxidant release device are respectively communicated with the first valve port and the second valve port of the first three-way valve. The third valve port of the first three-way valve, the outlet of the aerosol generation subsystem, and the outlet of the inlet side regulation module are respectively communicated with the first valve port, the second valve port, and the third valve port of the first four-way valve, and the fourth valve port of the first four-way valve is communicated with the inlet of the drum reaction chamber;

[0016] The outlet of the drum reaction chamber is communicated with the first valve port of the second four-way valve. The second valve port, the third valve port, and the fourth valve port of the second four-way valve are respectively communicated with the inlet of the outlet side regulation module, the inlet of the on-line measuring instrument, and the first valve port of the third three-way valve. The second valve port and the third valve port of the third three-way valve are respectively communicated with the inlet of the aerosol sampling instrument and the inlet of the particle size testing device.

[0017] The indoor aerosol test system further includes a second three-way valve; the disinfection liquid release device includes a first liquid storage tank, a liquid delivery pump, a liquid flow meter, and two disinfection liquid generation devices. The two disinfection liquid generation devices are respectively installed at the axial two ends of the drum reaction chamber. The first liquid storage tank stores disinfection liquid. The first liquid storage tank is communicated with the inlet of the liquid delivery pump. The outlet of the liquid delivery pump is communicated with the inlets of the two disinfection liquid generation devices through the second three-way valve. The outlets of the two disinfection liquid generation devices are both communicated with the inside of the drum reaction chamber. A liquid flow meter is arranged on the pipeline between the outlet of the liquid delivery pump and the second three-way valve.

[0018] The volatile organic compound generating device includes a zero-air generator, a second gas mass flow controller, a second liquid storage tank, and a syringe. The syringe is vertically arranged directly above the second liquid storage tank. On both sides of the outlet end of the syringe, a zero-air delivery pipeline and an organic matter delivery pipeline are arranged respectively. The outlet of the zero-air delivery pipeline and the inlet of the organic matter delivery pipeline are arranged opposite to each other and are both facing the outlet of the syringe. The inlet of the zero-air delivery pipeline is communicated with the outlet of the zero-air generator, and a second gas mass flow controller is arranged on the zero-air delivery pipeline. The outlet of the organic matter delivery pipeline is communicated with the first valve port of the first three-way valve;

[0019] The oxidant releasing device includes an oxidant generator and a third gas mass flow controller connected in sequence. The outlet of the third gas mass flow controller is communicated with the second valve port of the first three-way valve.

[0020] The on-line measuring instrument includes a carbon monoxide gas measuring instrument, a sulfur dioxide gas measuring instrument, and an ozone measuring instrument. The inlets of the carbon monoxide gas measuring instrument, the sulfur dioxide gas measuring instrument, and the ozone measuring instrument are connected in parallel and then communicated with the third valve port of the second four-way valve;

[0021] The aerosol sampling instrument includes an aerosol sampler, a fourth filter, and a second air pump connected in sequence. The inlet of the aerosol sampler is communicated with the second valve port of the third three-way valve;

[0022] The particle size testing device includes a particle size spectrometer and a computer. The inlet of the particle size spectrometer is communicated with the third valve port of the third three-way valve. The particle size spectrometer is internally provided with an air extraction device and a supporting gas path system, and the particle size spectrometer is electrically connected to the computer.

[0023] The aerosol sampler includes a solid sampler, a liquid sampler, or a membrane sampler.

[0024] The aerosol generating subsystem includes a gas generator, a pressure reducing valve, a first filter, a first gas mass flow controller, an aerosol production device, and a particle size screening device connected in series. The outlet of the particle size screening device is communicated with the second valve port of the first four-way valve;

[0025] The inlet side regulation module of the air flow regulation subsystem includes a membrane dryer, a temperature and humidity control device, and a second filter connected in series. The outlet of the second filter is communicated with the first valve port of the first four-way valve;

[0026] The outlet side regulation module of the air flow regulation subsystem includes a third filter, an air quality flowmeter, and a first air pump connected in series. The inlet of the third filter is communicated with the second valve port of the second four-way valve.

[0027] The test subsystem further includes an ultraviolet light control module and a temperature control compartment. The temperature control compartment is arranged outside the drum reaction chamber, and a temperature and humidity sensor is arranged at each of the inlet and outlet of the drum reaction chamber for measuring the temperature and humidity inside the drum reaction chamber in real time.

[0028] II. A method for disinfecting pathogen aerosols using the above multi-functional indoor aerosol test system

[0029] The test method includes the following steps:

[0030] S1. Connect the first valve port of the second four-way valve to the second valve port, and connect the second valve port and the third valve port of the first four-way valve to the fourth valve port. Generate virus aerosol using the aerosol generation subsystem and introduce it into the drum reaction chamber.

[0031] S2. After the ventilation ends, shut off the second valve port of the second four-way valve and the second valve port and the third valve port of the first four-way valve.

[0032] S3. Connect the third valve port of the first four-way valve to the fourth valve port, and connect the second valve port of the third three-way valve to the first valve port. Use the aerosol sampling instrument to perform initial aerosol sampling. After the aerosol sampling ends, shut off the second valve port of the third three-way valve and open the third valve port. Use the particle size testing device to perform initial particle size testing. After the particle size testing ends, shut off the third valve port of the third three-way valve and the third valve port of the first four-way valve.

[0033] S4. Connect the three valve ports of the second three-way valve, generate virus disinfectant solution using the disinfectant solution release device and introduce it into the drum reaction chamber. After the disinfection ends, close the second three-way valve.

[0034] S5. Perform post-disinfection aerosol sampling and post-disinfection particle size testing according to step S3.

[0035] S6. Analyze the results of the initial aerosol sampling and particle size testing, and the post-disinfection aerosol sampling and particle size testing to obtain the results of the pathogen aerosol disinfection test.

[0036] III. A method for testing secondary organic aerosols using the above multi-functional indoor aerosol test system

[0037] The test method includes the following steps:

[0038] S1. Connect the first valve port of the second four-way valve to the second valve port, connect the first valve port of the first four-way valve to the fourth valve port, connect the first valve port of the first three-way valve to the third valve port, open the valve of the first air pump, and use the zero gas generator in the volatile organic compound generation device to clean the drum reaction chamber.

[0039] S2. After the cleaning ends, close the first valve port and the third valve port of the first three-way valve.

[0040] S3. Meanwhile, connect both the second valve port and the third valve port of the first four-way valve to the fourth valve port, generate virus aerosol using the aerosol generation subsystem and introduce it into the rotary drum reaction chamber. After the ventilation ends, shut off the second valve port of the first four-way valve.

[0041] S4. Connect both the first valve port and the third valve port of the first four-way valve to the fourth valve port, connect the second valve port of the first three-way valve to the third valve port, introduce oxidant into the rotary drum reaction chamber using the oxidant release device. After the ventilation ends, shut off the second valve port of the first three-way valve.

[0042] S5. Connect both the first valve port and the third valve port of the first four-way valve to the fourth valve port, connect the first valve port of the first three-way valve to the third valve port, introduce the gas containing volatile organic compounds into the rotary drum reaction chamber using the volatile organic compound generation device. After the ventilation ends, simultaneously shut off the second valve port of the second four-way valve, the first valve port of the first four-way valve, and the first valve port of the first three-way valve.

[0043] S6. Mix all reactants in the rotary drum reaction chamber, turn on the ultraviolet light control module to carry out the photo-oxidation reaction. During the reaction process, connect the third valve port of the second four-way valve to the first valve port to detect the concentration changes of carbon monoxide, sulfur dioxide, and ozone in the rotary drum reaction chamber in real time.

[0044] S7. After the photo-oxidation reaction ends, connect the fourth valve port of the second four-way valve to the first valve port, connect the second valve port of the third three-way valve to the first valve port, and use the aerosol sampling instrument to conduct aerosol sampling. After the aerosol sampling ends, shut off the second valve port of the third three-way valve and open the third valve port, use the particle size testing device to conduct the initial particle size test. After the particle size test ends, shut off the third valve port of the third three-way valve and the third valve port of the first four-way valve.

[0045] S8. Analyze the aerosol sampling results and the particle size test results to obtain the test results of secondary organic aerosol.

[0046] The beneficial effects of the present invention are as follows:

[0047] 1. As a multifunctional aerosol test system, this system can realize the disinfection test of pathogens in building interiors, determine the effects of different types, proportions of disinfection liquids, and disinfection times on the survival rates of virus and bacterial pathogen aerosols, and determine the effects of ultraviolet light intensity and irradiation time on the survival rates of virus and bacterial pathogen aerosols. The multifunctional aerosol test system can also be used as an indoor secondary organic aerosol synthesis test system to explore the effects of related factors such as indoor particulate matter concentration, types and concentrations of oxidants, and ultraviolet light intensity on the synthesis components, particle size distribution, and concentration of indoor secondary organic aerosols.

[0048] 2. In the indoor pathogen disinfection test, the system can perform PCR assays and TCID 50 assays on the aerosol samples collected by the sampler, and compare the quantitative change in the activity attenuation of pathogen aerosols before and after the test.

[0049] 3. In the secondary organic aerosol synthesis test, the system can collect particulate matter samples using quartz filter membranes. After sampling, the gas and aerosol membrane sampling inlet are introduced into a high-resolution time-of-flight chemical ionization mass spectrometer through thermal desorption for the identification of particulate phase products. Meanwhile, the SMPS scanning mobility particle sizer measures the concentration and particle size distribution of the secondary organic aerosol after the reaction, and the yield of the secondary organic aerosol can be calculated. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] Figure 1 It is a schematic diagram of a multi-functional aerosol test system;

[0051] Figure 2 It is a diagram of the internal structure of the rotary drum reaction chamber;

[0052] Figure 3 It is a schematic diagram of the pathogen disinfection test system;

[0053] Figure 4 It is a schematic diagram of the secondary organic aerosol synthesis test system.

[0054] In the figure, 10. aerosol generation subsystem; 11. gas generator; 12. pressure reducing valve; 13. first filter; 14. first gas mass flow controller; 15. aerosol production equipment; 16. particle size screening device; 20. air flow regulation subsystem; 21. membrane dryer; 22. constant temperature and humidity control equipment; 23. second filter; 24. third filter; 25. air quality flow meter; 26. first air pump; 30. disinfection liquid release equipment; 31. first liquid storage tank; 32. liquid transfer pump; 33. liquid flow meter; 34. disinfection liquid production equipment; 40. volatile organic compound production equipment; 41. zero air generator; 42. second gas mass flow controller; 43. second liquid storage tank; 44. syringe; 50. oxidant release equipment; 51. oxidant generator; 52. third gas mass flow controller; 60. test subsystem; 61. ultraviolet light control module; 62. rotary drum reaction chamber; 63. temperature control compartment; 70. detection and monitoring subsystem; 71. temperature and humidity sensor; 72. carbon monoxide gas detector; 73. sulfur dioxide gas detector; 74. ozone detector; 75. aerosol sampler; 76. fourth filter; 77. second air pump; 78. particle sizer; 79. computer; 81. first three-way valve; 82. second three-way valve; 83. first four-way valve; 84. second four-way valve; 85. third three-way valve. Detailed implementation manners

[0055] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0056] The first aspect of the present invention provides a multifunctional indoor aerosol test system.

[0057] The test system of the present invention includes:

[0058] A test subsystem 60, including a drum reaction chamber 62;

[0059] An aerosol generation subsystem 10 for inputting aerosol into the test subsystem 60;

[0060] An air flow regulation subsystem 20, including an inlet side regulation module and an outlet side regulation module, which are respectively arranged on the inlet side and the outlet side of the drum reaction chamber 62;

[0061] A test substance release subsystem, including a disinfection liquid release device 30, a volatile organic compound generation device 40 and an oxidant release device 50, for inputting test substances into the test subsystem 60, and the test substances include one or more of disinfection liquid, volatile organic compounds (VOCs) and oxidants;

[0062] A detection and monitoring subsystem 70 for detecting and monitoring the changes of relevant parameters in the test subsystem in real time, including on-line measuring instruments, aerosol sampling instruments and particle size testing devices.

[0063] Further, one end of the drum reaction chamber 62 is connected to aerosol, regulated air flow, pathogen disinfection liquid, VOCs and oxidants, and each pipeline exists independently of each other and is controlled by a valve. The other end of the drum is connected to a first air extraction pump 26 and a detection and monitoring subsystem 70.

[0064] Further, as Figure 1As shown in the figure, the indoor aerosol test system further includes a first three-way valve 81, a third three-way valve 85, a first four-way valve 83, and a second four-way valve 84; the outlets of the volatile organic compound generating device 40 and the oxidant releasing device 50 are respectively communicated with the first valve port (right) and the second valve port (down) of the first three-way valve 81, and the third valve port (left) of the first three-way valve 81, the outlet of the aerosol generating subsystem 10, and the outlet of the inlet side regulation module are respectively communicated with the first valve port (right), the second valve port (up), and the third valve port (down) of the first four-way valve 83, and the fourth valve port (left) of the first four-way valve 83 is communicated with the inlet of the rotary drum reaction chamber 62; the outlet of the rotary drum reaction chamber 62 is communicated with the first valve port (right) of the second four-way valve 84, and the second valve port (up), the third valve port (down), and the fourth valve port (left) of the second four-way valve 84 are respectively communicated with the inlet of the outlet side regulation module, the inlet of the on-line measuring instrument, and the first valve port (right) of the third three-way valve 85, and the second valve port (down) and the third valve port (left) of the third three-way valve 85 are respectively communicated with the inlet of the aerosol sampling instrument and the inlet of the particle size testing device.

[0065] Furthermore, the indoor aerosol test system further includes a second three-way valve 82. The disinfection liquid releasing device 30 includes a first liquid storage tank 31, a liquid delivery pump 32, a liquid flow meter 33, and two disinfection liquid generating devices 34. The two disinfection liquid generating devices 34 are respectively installed at the axial two ends of the rotary drum reaction chamber 62. The first liquid storage tank 31 stores the disinfection liquid. The first liquid storage tank 31 is communicated with the inlet of the liquid delivery pump 32. The outlet of the liquid delivery pump 32 is communicated with the inlets of the two disinfection liquid generating devices 34 through the second three-way valve 82. The outlets of the two disinfection liquid generating devices 34 are both communicated with the inside of the rotary drum reaction chamber 62. A liquid flow meter 33 is arranged on the pipeline between the outlet of the liquid delivery pump 32 and the second three-way valve 82. After the liquid delivery pump 32 pumps the disinfection liquid stored in the first liquid storage tank 31 through the liquid flow meter 33, it is divided into two paths through the second three-way valve 82 and respectively flows into the two disinfection liquid generating devices 34. The atomized disinfection liquid generated by the disinfection liquid generating device 34 is introduced into the rotary drum reaction chamber 62 of the test subsystem.

[0066] In specific implementation, the outlet of the disinfection liquid generating device 34 can be communicated with the inside of the rotary drum reaction chamber 62 by extending the nozzle of the disinfection liquid generating device 34 into the rotary drum reaction chamber 62.

[0067] Preferably, the disinfection liquid generating device 34 is selected as a hollow cone nozzle. The hollow cone nozzle relies on the spiral groove on the internal chip of the nozzle to make the working medium generate a swirl. When the liquid flows to the nozzle orifice, it is ejected in the form of a hollow cone through a curved surface to achieve uniform atomization.

[0068] Specifically, the volatile organic compound generating device 40 includes a zero-air generator 41, a second gas mass flow controller 42, a second liquid reservoir 43, and a syringe 44. The syringe 44 is vertically arranged directly above the second liquid reservoir 43. On both sides of the outlet end of the syringe 44, a zero-air delivery pipeline and an organic matter delivery pipeline are arranged. The outlet of the zero-air delivery pipeline and the inlet of the organic matter delivery pipeline are arranged opposite to each other and are both facing the outlet of the syringe 44. The inlet of the zero-air delivery pipeline is communicated with the outlet of the zero-air generator 41, and the second gas mass flow controller 42 is arranged on the zero-air delivery pipeline. The outlet of the organic matter delivery pipeline is communicated with the first valve port of the first three-way valve 81. After the zero air generated by the zero-air generator 41 passes through the second gas mass flow controller 42, it purges the volatile organic compound standard solution injected into the second liquid reservoir 43 by the micro syringe 44, generates a gas containing volatile organic compounds, and flows out from the outlet of the organic matter delivery pipeline.

[0069] The oxidant releasing device 50 includes an oxidant generator 51 and a third gas mass flow controller 52 that are connected in sequence. The outlet of the third gas mass flow controller 52 is communicated with the second valve port of the first three-way valve 81. The oxidant generated by the oxidant generator 51 flows out from the outlet of the third gas mass flow controller 52 after passing through the third gas mass flow controller 52.

[0070] Preferably, the oxidant releasing device 50 generates an atomized disinfection liquid.

[0071] Specifically, the on-line measuring instrument includes a carbon monoxide gas measuring instrument 72, a sulfur dioxide gas measuring instrument 73, and an ozone measuring instrument 74. The inlets of the carbon monoxide gas measuring instrument 72, the sulfur dioxide gas measuring instrument 73, and the ozone measuring instrument 74 are connected in parallel and then communicated with the third valve port of the second four-way valve 84. The on-line measuring instrument can measure the concentration changes of various gases in the drum reaction chamber 62 in real time. The on-line measuring instrument can measure the gas concentration in the drum reaction chamber during the test, capture the changes in the physicochemical characteristics of the pollutants, and the reaction conditions.

[0072] Specifically, the aerosol sampling instrument includes an aerosol sampler 75, a fourth filter 76, and a second air pump 77 that are connected in sequence. The inlet of the aerosol sampler 75 is communicated with the second valve port of the third three-way valve 85. During the sampling process, the aerosol at the outlet of the synthesis chamber passes through the aerosol sampler 75, the fourth filter 76, and the second air pump 77 in sequence and then is discharged. The aerosol sampler 75 is used to collect sampling samples of virus aerosols for virus activity and absolute quantification determination, or collect sampling samples of secondary organic aerosols and analyze the composition of the particulate phase products of secondary organic aerosols through a mass spectrometry analyzer.

[0073] Specifically, the particle size testing device includes a particle size spectrometer 78 and a computer 79. The inlet of the particle size spectrometer 78 is communicated with the third valve port of the third three-way valve 85. The particle size spectrometer 78 is internally provided with a pumping device and a supporting gas path system. The particle size spectrometer 78 is electrically connected to the computer 79. The particle size spectrometer 78 is used to measure the number concentration and particle size distribution of particulate matters in the aerosol in real time and dynamically.

[0074] Specifically, the particle size spectrometer 78 includes an aerodynamic spectrometer and a scanning mobility particle sizer. The aerodynamic spectrometer can measure the number concentration and particle size distribution of particulate matters with an aerodynamic diameter in the range of 0.3 - 100 μm in the aerosol in real time and dynamically. The scanning mobility particle sizer can measure the number concentration and particle size spectrum distribution of particulate matters in the range of 1 - 1000 nm during the reaction process. The particle size spectrometer 78 is communicated with the particle size spectrometer interface through a pipeline, and the particle size spectrometer 78 is electrically connected to the computer.

[0075] The aerosol sampler 75 can collect sampling samples of pathogen aerosol for virus activity and absolute quantitative determination, or collect sampling samples of secondary organic aerosol for analyzing the composition of the particulate phase products of secondary organic aerosol by a mass spectrometry analyzer.

[0076] Preferably, the aerosol sampler 75 includes a solid sampler, a liquid sampler or a membrane sampler.

[0077] Preferably, the solid impact sampler mainly includes Andersen sampler, slit sampler and cyclone sampler. The liquid sampler mainly includes AGI - 30 sampler and BioSampler. The sampling principle and filtration efficiency of the membrane sampler are mainly based on interception, inertial collision, diffusion, gravitational sedimentation and electrostatic attraction. The samplers for secondary organic aerosol mainly adopt membrane samplers and solid phase adsorption tubes, and the sampling samples are then pyrolytically desorbed and introduced into the mass spectrometry analyzer to determine the composition of the particulate phase products of secondary organic aerosol.

[0078] Specifically, the aerosol generation subsystem 10 includes a gas generator 11, a pressure reducing valve 12, a first filter 13, a first gas mass flow controller 14, an aerosol production device 15 and a particle size screening device 16 connected in series. The outlet of the particle size screening device 16 is communicated with the second valve port of the first four-way valve 83. The air flow generated by the gas generator 11 passes through the outlet of the pressure reducing valve 12, successively flows through the first filter 13 and the first gas mass flow controller 14, and then enters the aerosol production device 15. The aerosol generated by the aerosol production device 15 is subjected to particle size screening by the particle size screening device 16 and then flows out from the outlet of the particle size screening device 16.

[0079] Specifically, the inlet - side regulation module of the air - flow regulation subsystem includes a membrane dryer 21, a temperature - and - humidity control device 22, and a second filter 23 connected in series. The outlet of the second filter 23 is communicated with the first valve port of the first four - way valve 83. The temperature - and - humidity control device 22 is internally provided with an air pump. The inlet of the membrane dryer 21 is communicated with the atmosphere or a gas source. The gas output from the atmosphere air or the gas source passes through the membrane dryer 21, then passes through the temperature - and - humidity control device 22 to generate a regulated air flow, and the regulated air flow flows out after passing through the second filter 23.

[0080] Specifically, the outlet - side regulation module of the air - flow regulation subsystem includes a third filter 24, an air - quality flowmeter 25, and a first air - extraction pump 26 connected in series. The inlet of the third filter 24 is communicated with the second valve port of the second four - way valve 84. The outlet of the first air - extraction pump 26 is communicated with the atmosphere, a gas - treatment device, or a gas - collection device.

[0081] Preferably, the aerosol production device 15 is a compressed atomizer. Compressed atomization utilizes Bernoulli's principle. The negative pressure brought by the high - speed gas promotes the liquid to rise and then be ejected with the high - speed air flow, and impacts the blocking wall surface to form droplets of different sizes. Then, secondary screening is carried out using gravity, so that most large particles (>10μm) fall back, and smaller particulate matter is ejected from the outlet. The virus and bacteria aerosols produced by the aerosol production device can be used for pathogen disinfection tests, and the seed aerosols produced by the aerosol production device can be used for secondary organic aerosol synthesis tests.

[0082] Preferably, the particle - size screening device 16 is an impactor. The main function of the impactor is to screen seed aerosols smaller than a specific size, and its working principle is based on the inertial impact effect exhibited by the seed aerosols during movement. When the seed aerosols pass through the slit - type nozzles provided on each impact plate, the air flow deflects 90° in the streamline direction. Larger droplets than the cut - off diameter cannot continue to move along the streamline due to inertia, so they directly impact and are captured by the collection medium, while the seed aerosols smaller than the cut - off diameter bypass the sampling plate with the air flow and enter the next - level screening process, finally screening out aerosols smaller than a specific size.

[0083] The first air - extraction pump 26 promotes the aerosol produced by the aerosol production subsystem 10 to be uniformly mixed with the regulated air flow flowing out of the temperature - and - humidity control device 22 through air extraction and react in the rotating - drum reaction chamber 62. After the reaction, the first air - extraction pump 26 discharges the test system through the third filter (24) by air extraction.

[0084] Preferably, the temperature - and - humidity control device 22 is a temperature - and - humidity test chamber, and the temperature - and - humidity test chamber is equipped with heating, refrigeration, humidification, and dehumidification systems.

[0085] Further, the test subsystem further includes an ultraviolet light control module 61 and a temperature control compartment 63. The temperature control compartment 63 is arranged outside the drum reaction chamber 62, and a temperature and humidity sensor 71 is arranged at each of the entrance and exit of the drum reaction chamber 62 for real-time measurement of the temperature and humidity inside the drum reaction chamber 62.

[0086] The pathogen aerosol entering the test subsystem is subjected to ultraviolet irradiation disinfection under the ultraviolet light with a wavelength of 10-400 nm controlled by the ultraviolet light control module 61, and the change of the disinfection ability at different ultraviolet intensities with the residence time can be tested. In addition, the VOCs entering the test subsystem undergo a secondary organic aerosol reaction under the ultraviolet light with the corresponding wavelength controlled by the ultraviolet light control module 61. After the secondary organic aerosol synthesis reaction, the secondary organic aerosol particles can be kept suspended for a long time in the rotatable drum reaction chamber 62. Therefore, sampling and monitoring of the drum reaction chamber can be realized at different suspension durations, and then the components of the secondary organic aerosol particles can be analyzed. Moreover, the effects of factors such as temperature, humidity and ultraviolet light intensity on the synthesis concentration and particle size of the secondary organic aerosol can be studied simultaneously.

[0087] Further, the ultraviolet lamp in the ultraviolet light control module 61 is screwed into the drum entrance and exit, and the ultraviolet lamp can be disassembled and assembled at any time according to the reaction conditions.

[0088] Further, the temperature control compartment 63 is arranged outside the drum reaction chamber 62, which can solve the problem of heat leakage during long-term operation and improve the temperature uniformity and stability inside the drum reaction chamber. In the experiment, the specific temperature control can be jointly controlled by introducing an adjusted air flow at a set temperature and the temperature control compartment, or multiple methods can be combined to jointly achieve temperature control.

[0089] The second aspect of the present invention provides a method for disinfecting pathogen aerosol using the above-mentioned multifunctional indoor aerosol test system. In the pathogen disinfection test, the first air pump is turned on, and the constant temperature and humidity adjusted air flow generated by the air flow adjustment subsystem is continuously introduced into the drum reaction chamber. At the same time, the gas generator is turned on and the gas flow rate entering the aerosol generating device is controlled, and the pathogen aerosol is continuously atomized and introduced into the drum reaction chamber. After a period of time, the valves connecting the adjusted air flow and the first air pump are closed simultaneously. After the pathogen aerosol is suspended in the drum for a set time, the liquid delivery pump is turned on to deliver the pathogen disinfectant liquid into the disinfectant liquid generating device to generate a spray and enter the test subsystem for disinfecting the pathogen aerosol, or the ultraviolet lamp in the test subsystem is turned on to disinfect the pathogen aerosol.

[0090] The test method of the present invention specifically includes the following steps:

[0091] S1. Start the constant temperature and humidity control device 22, the first air extraction pump 26, and the drum motor of the drum reaction chamber 62. By opening the second valve port of the second four-way valve 84 and the second and third valve ports of the first four-way valve 83, connect the first valve port of the second four-way valve 84 to the second valve port, and both the second and third valve ports of the first four-way valve 83 are connected to the fourth valve port, so that the outlet of the aerosol generation subsystem 10 and the outlet of the inlet and outlet side regulation module are both connected to the inlet of the drum reaction chamber 62, and the outlet of the drum reaction chamber 62 is connected to the inlet of the outlet side regulation module. Generate virus aerosol using the aerosol generation subsystem 10 and introduce it into the drum reaction chamber 62;

[0092] S2. After the ventilation ends, close the second valve port of the second four-way valve 84 and the second and third valve ports of the first four-way valve 83, turn off the gas generator (11), close the inlet and outlet valves of the drum reaction chamber 62, and turn off the first air extraction pump 26;

[0093] S3. Open the third valve port of the first four-way valve 83 and the second valve port of the third three-way valve 85, connect the third valve port of the first four-way valve 83 to the fourth valve port, and connect the second valve port of the third three-way valve 85 to the first valve port, so that the outlet of the drum reaction chamber 62 is connected to the inlet of the aerosol sampling instrument. Perform initial aerosol sampling using the aerosol sampling instrument. After the aerosol sampling is completed, close the second valve port of the third three-way valve 85 and open the third valve port, so that the outlet of the drum reaction chamber 62 is connected to the inlet of the particle size testing device. Perform initial particle size testing using the particle size testing device. After the particle size testing is completed, close the third valve port of the third three-way valve 85 and the third valve port of the first four-way valve 83;

[0094] S4. By opening the second three-way valve 82, connect the three valve ports of the second three-way valve 82. Generate virus disinfection liquid using the disinfection liquid release device 30 and introduce it into the drum reaction chamber 62. After the disinfection is completed, close the second three-way valve 82;

[0095] S5. Perform post-disinfection aerosol sampling and post-disinfection particle size testing according to step S3;

[0096] S6. Analyze the results of the initial aerosol sampling and particle size testing, and the post-disinfection aerosol sampling and particle size testing to obtain the pathogen aerosol disinfection test results.

[0097] The third aspect of the present invention provides a method for testing secondary organic aerosols using the above-mentioned multi-functional indoor aerosol test system. In the synthesis test of secondary organic aerosols, after the zero air generated by the zero air generator washes the rotating drum reaction chamber by turning on the first air pump, the constant temperature and humidity regulated air flow generated by the air flow regulating subsystem is continuously introduced into the rotating drum reaction chamber to simulate the temperature and humidity of the atmospheric environment. Then, the valves connecting the regulating air flow and the first air pump are closed simultaneously, and the VOCs generated by the VOCs generating device in the test substance release subsystem are introduced into the rotating drum reaction chamber. The valve is switched, and the oxidant released in the test substance release subsystem is introduced into the rotating drum reaction chamber. The ultraviolet light control system turns on the switch of the ultraviolet lamp with the corresponding wavelength to synthesize secondary organic aerosols. After the synthesis of secondary organic aerosols, they can be suspended in the rotating drum for a long time.

[0098] The test method of the present invention includes the following steps:

[0099] S1. Start the constant temperature and humidity control device 22, the first air pump 26 and the drum motor of the rotating drum reaction chamber 62. By opening the second valve port of the second four-way valve 84, the first valve port of the first four-way valve 83 and the first valve port of the first three-way valve 81, connect the first valve port and the second valve port of the second four-way valve 84, connect the first valve port and the fourth valve port of the first four-way valve 83, and connect the first valve port and the third valve port of the first three-way valve 81, so that the outlet of the volatile organic compound generating device 40 is connected to the inlet of the rotating drum reaction chamber 62, the outlet of the rotating drum reaction chamber 62 is connected to the inlet of the outlet side regulation module, open the valve of the first air pump 26, and use the zero air generator 41 in the volatile organic compound generating device 40 to clean the rotating drum reaction chamber 62;

[0100] S2. After the cleaning is completed, by opening the third valve port of the first four-way valve 83, cut off the first valve port of the first four-way valve 83 and the first valve port of the first three-way valve 81, and connect the third valve port and the fourth valve port of the first four-way valve 83, so that the outlet of the inlet side regulation module is connected to the inlet of the rotating drum reaction chamber 62, and the outlet of the rotating drum reaction chamber 62 is connected to the inlet of the outlet side regulation module;

[0101] S3. By opening the second valve port of the first four-way valve 83, connect the second valve port and the third valve port of the first four-way valve 83 to the fourth valve port, so that the outlet of the aerosol generating subsystem 10 and the outlet of the inlet side regulation module are both connected to the inlet of the rotating drum reaction chamber 62, generate virus aerosol using the aerosol generating subsystem 10 and introduce it into the rotating drum reaction chamber 62. After the ventilation is completed, cut off the second valve port of the first four-way valve 83;

[0102] S4. By simultaneously opening the first valve port of the first four-way valve 83 and the second valve port of the first three-way valve 81, the first valve port and the third valve port of the first four-way valve 83 are both connected to the fourth valve port, and the second valve port of the first three-way valve 81 is connected to the third valve port, so that the outlet of the oxidant release device 50 and the outlet of the inlet-side regulation module are simultaneously connected to the inlet of the rotary drum reaction chamber 62. The oxidant is introduced into the rotary drum reaction chamber 62 by the oxidant release device 50. After the ventilation is completed, the second valve port of the first three-way valve 81 is shut off;

[0103] S5. By simultaneously opening the first valve port of the first four-way valve 83 and the first valve port of the first three-way valve 81, the first valve port and the third valve port of the first four-way valve 83 are both connected to the fourth valve port, and the first valve port of the first three-way valve 81 is connected to the third valve port, so that the volatile organic compound generation device 40 and the outlet of the inlet-side regulation module are simultaneously connected to the inlet of the rotary drum reaction chamber 62. The gas containing volatile organic compounds is introduced into the rotary drum reaction chamber 62 by the volatile organic compound generation device 40. After the ventilation is completed, the second valve port of the second four-way valve 84, the first valve port of the first four-way valve 83, and the first valve port of the first three-way valve 81 are simultaneously shut off;

[0104] S6. All reactants are mixed in the rotary drum reaction chamber 62, and the ultraviolet light control module 61 is turned on to carry out a photo-oxidation reaction. During the reaction, by opening the third valve port of the second four-way valve 84 and connecting the third valve port of the second four-way valve 84 to the first valve port, the inlet of the on-line measuring instrument is connected to the outlet of the rotary drum reaction chamber 62 to detect the concentration changes of carbon monoxide, sulfur dioxide, and ozone in the rotary drum reaction chamber 62 in real time;

[0105] S7. After the photo-oxidation reaction is completed, by opening the fourth valve port of the second four-way valve 84 and the second valve port of the third three-way valve 85, the fourth valve port of the second four-way valve 84 is connected to the first valve port, and the second valve port of the third three-way valve 85 is connected to the first valve port, so that the inlet of the aerosol sampling instrument is connected to the outlet of the detection rotary drum reaction chamber 62, and the aerosol sampling instrument is used for aerosol sampling. After the aerosol sampling is completed, the second valve port of the third three-way valve 85 is shut off and the third valve port is opened, so that the outlet of the rotary drum reaction chamber 62 is connected to the inlet of the particle size testing device, and the initial particle size is tested by the particle size testing device. After the particle size testing is completed, the third valve port of the third three-way valve 85 and the third valve port of the first four-way valve 83 are shut off;

[0106] S8. Analyze the aerosol sampling results and the particle size testing results to obtain the secondary organic aerosol test results.

[0107] The specific embodiments of the present invention are as follows:

[0108] In the following embodiments, only a part of the flow paths of the test system of the present invention are utilized. To simplify the illustration, the unused flow paths are omitted in the figures. This representation method aims to highlight the actual flow path of the system under the current working conditions.

[0109] Embodiment 1

[0110] As Figure 3 shown, in this embodiment, the test system of the present invention is used for the disinfection test of pathogen aerosol. The equipment used in this embodiment is as follows:

[0111] ①Aerosol generation subsystem 10

[0112] The aerosol generation subsystem 10 includes a gas generator 11, a pressure reducing valve 12, a first filter 13, a first gas mass flow controller 14, an aerosol production device 15, and a particle size screening device 16.

[0113] The gas generator 11 selects a compressed air cylinder.

[0114] The first filter 13 is a HEPA membrane filter, which can filter particles with a diameter of more than 0.22 μm in the compressed air flowing out of the pressure reducing valve, effectively filtering the interference of the inlet gas on the subsequent test results.

[0115] The aerosol production device 15 selects a Nebulizer atomizer produced by BGI. Due to the low pressure generated by the high-speed air flow, the liquid is carried out. The liquid collides with the wall of the pressure-resistant glass bottle at a high speed to generate fine liquid droplets, forming a saturated aerosol.

[0116] The preferred working flow rate of the Nebulizer atomizer is 6.1 L / min. The particle size screening device 16 selects a PM10 impact sampler from Dekati, Finland, with a screening target of 10 μm, and screens aerosol with a size of less than 10 μm into the drum reaction chamber 62.

[0117] The gas in the compressed air cylinder flows through the HEPA membrane filter through the pressure reducing valve 12, and flows into the Nebulizer atomizer containing 15 mL of influenza A H1N1 virus solution through the first gas mass flow controller 14. The pressure reducing valve 12 and the first gas mass flow controller simultaneously control the air flow rate of 6.1 L / min into the Nebulizer atomizer to generate a uniform virus aerosol. After the virus aerosol is screened by the PM10 impact sampler, it flows out of the aerosol generation subsystem 10.

[0118] ②Airflow regulation subsystem 20

[0119] The airflow regulation subsystem 20 includes a membrane dryer 21, a constant temperature and humidity control device 22, a second filter 23, a third filter 24, a gas mass flowmeter 25, and a first air pump 26.

[0120] The constant temperature and humidity control device 22 selects the DHT-100 constant temperature and humidity chamber of Duohe Test Co., Ltd., with a volume of 100L. Its temperature and humidity range is 0-120°C, 5%-98% relative humidity, the temperature fluctuation is ±0.1 to ±0.5°C, the uniformity is ±0.5 to ±1.5°C, and the humidity fluctuation is ±1.0% to ±2.0%.

[0121] Both the second filter 23 and the third filter 24 are HEPA membrane filters.

[0122] The gas mass flowmeter 25 selects a glass rotor flowmeter.

[0123] Adjust the air flow to enter the constant temperature and humidity control box 22 after passing through the membrane dryer 21, flow through the HEPA membrane filter, and flow out of the air flow control subsystem 20. Adjust the air flow to mix with the above-mentioned virus aerosol to control the temperature and humidity of the air flow entering the test subsystem, forming an adjusted virus aerosol. The first air pump 26 promotes the uniform mixing of the atomized air flow of the above-mentioned virus aerosol and the adjusted air flow in the drum reaction chamber by pumping.

[0124] ③ Test substance release subsystem

[0125] The pathogen disinfection liquid release device includes a first liquid storage tank 31, a liquid delivery pump 32, a liquid flowmeter 33, and a disinfection liquid generation device 34.

[0126] The liquid delivery pump 32 for the disinfection liquid selects a water pumping diaphragm pump.

[0127] The disinfection liquid generation device 34 selects the hollow cone nozzle Series 220 of Lechler Company.

[0128] After the adjusted virus aerosol suspends in the drum reaction chamber 62 for a set time, the water pumping diaphragm pump pumps the virus disinfection liquid in the first liquid storage tank 31 through the liquid flowmeter 33 and then flows into the hollow cone nozzle, and the hollow cone nozzle generates fine spray and directly sprays into the drum reaction chamber 62.

[0129] ④ Test subsystem 60

[0130] The test subsystem 60 includes an ultraviolet light control module 61, a drum reaction chamber 62, and a temperature control compartment 63. In this embodiment, to explore the disinfection rate of the disinfection liquid on the pathogen aerosol, the ultraviolet light control module 61 is detachable.

[0131] The entire drum reaction chamber 62 is made of aluminum alloy as the processing material, and the transmission method is selected as gear and roller transmission, so that the drum body maintains a stable rotation speed. The outer diameter of the drum is 260 mm, the wall thickness is 5 mm, and the length is 300 mm. The main body of the rotary drum consists of a shell, an end cover, a shell connecting piece, a support shaft and a central shaft. A plurality of sealing rings are arranged radially, and a combination of a sealed bearing and an oil seal is adopted axially to ensure airtightness. The inner surface of the rotary drum is made of polytetrafluoroethylene material, and the outer surface of the polytetrafluoroethylene cylindrical tube is attached to the inner surface of the rotary drum after chemical treatment. The rotary drum rotates at a speed of 1-3 rpm (the speed can be adjusted by the motor) to maintain the suspended state of the virus aerosol. Within this speed range, the natural sedimentation rate of the virus aerosol is significantly reduced, and it can maintain a relatively stable suspended state within 3 hours.

[0132] The temperature control compartment 63 selects an electrothermal blast thermostatic oven from Huadong Instruments, with a maximum heating power of 1500 W, a temperature control range of room temperature to 80 °C, a temperature fluctuation of ±0.2 °C, and a setting accuracy of 0.1 °C.

[0133] ⑤ Detection and monitoring subsystem 70

[0134] The detection and monitoring subsystem 70 includes two temperature and humidity sensors 71, an aerosol sampling instrument and a particle size testing device. The aerosol sampling instrument includes an aerosol sampler 75, a fourth filter 76, and a second air pump 77. The particle size testing device includes a particle size spectrometer 78 and a computer 79.

[0135] The temperature and humidity sensor 71 selects the Vaisala HMT-120 temperature and humidity transmitter and is respectively set at the entrance and exit of the drum reaction chamber to measure the temperature and humidity in the drum reaction chamber in real time.

[0136] The virus aerosol sampler 75 selects the SKC BioSampler liquid sampler, which uses the inertial force of particles to capture particles into the liquid. It is suitable for sampling bacteria and virus aerosols, can maintain high activity, and the sampling flow rate is 12.5 L / min.

[0137] The second air pump 77 selects the SKC QuickTake30 six-stage sieve hole impact air microorganism sampler, which has the advantages of small volume, light weight and good stability. The maximum working flow rate is 30 L / min.

[0138] The fourth filter 76 selects a HEPA membrane filter.

[0139] The particle size spectrometer 78 selects the TSI TSIAPS-3321 aerodynamic spectrometer of the TSI company, which can dynamically measure the number concentration and particle size distribution of virus aerosols with an aerodynamic diameter in the range of 0.3-100 μm in the virus aerosol. The particle size spectrometer is connected to the particle size spectrometer interface through a pipeline, and the particle size spectrometer is electrically connected to the computer.

[0140] ⑥ Valve assembly

[0141] The first three-way valve 81, the second three-way valve 82 and the third three-way valve 85 are all three-way valves. A three-way valve connects one passage and two branches with independent control switches.

[0142] The first four-way valve 83 and the second four-way valve 84 are both four-way valves. A four-way valve connects one passage and three branches with independent control switches.

[0143] The following are the specific steps for conducting the aerosol disinfection test of the influenza A (H1N1) virus at room temperature of 25°C:

[0144] 1: Start the constant temperature and humidity chamber, set it to the set temperature of 25°C and the relative humidity of 50%, until the temperature and humidity reach stability.

[0145] 2: Set the output speed of the drum variable frequency motor to 60 rpm, and at this time the drum speed is 2 rpm.

[0146] 3: Open the upper branch switch of the second four-way valve 84, and at the same time open the upper and lower branch switches of the first four-way valve 83. Turn on the first air pump and set the flow rate to 30 L / min.

[0147] 4: Open the first mass flow controller and the compressed air cylinder, adjust the atomization air flow rate to 6.1 L / min, continuously atomize the influenza A (H1N1) virus solution for 5 min, and at the same time compare the front and rear temperature and humidity sensors to reduce the non-uniform distribution.

[0148] 5: After ventilating for 5 min, close the upper and lower branch switches of the first four-way valve 83 and the upper branch switch of the second four-way valve 84 at the same time, quickly close the compressed air cylinder, the front and rear valves of the drum and the air pump. Before introducing the virus disinfection liquid, open the lower branch switch controlled by the first four-way valve 83, open the left branch switch controlled by the third three-way valve 85, and the aerodynamic particle sizer conducts sampling for 5 s. Immediately afterwards, open the lower branch switch of the third three-way valve 85, use the SKC membrane sampler to conduct air sampling, set the sampling flow rate to 5 L / min, and the sampling time is 3 min. Then close the lower branch switch of the third three-way valve 85. Finally, the aerodynamic particle sizer conducts sampling for 5 s, close the left branch switch of the third three-way valve 85, and the liquid sampler collects a liquid volume of 5 ml. Subsequently, the collected liquid is aliquoted into centrifuge tubes and transferred to a refrigerator (4°C) for storage.

[0149] 6: Open the upper and lower branch switches of the second three-way valve 82, turn on the pumping diaphragm pump to pump the virus disinfection liquid in the liquid storage tank through the liquid flow meter into the nozzle. The hollow cone nozzle generates fine spray and directly sprays into the drum. The flow rate of the nozzle during operation is 0.39 L / min, and it continuously atomizes for 5 min.

[0150] 7: Quickly close the upper and lower branch switches of the second three-way valve 82, and at the same time, turn off the water pumping diaphragm pump. First, sample with an aerodynamic spectrometer. Open the lower branch switch controlled by the first four-way valve 83 and the left branch switch controlled by the third three-way valve 85. The sampling time is 5 s. Immediately afterwards, open the lower branch switch of the third three-way valve 85, and then use the SKC membrane liquid sampler for air sampling. The sampling flow rate is set at 5 L / min, and the sampling time is 3 min. Finally, sample with the aerodynamic spectrometer for 5 s. Close the left and lower branch switches of the third three-way valve 85. The volume of the liquid collected by the liquid sampler is 5 ml. Subsequently, sub-pack the collected liquid into centrifuge tubes and transfer them to a refrigerator for storage.

[0151] 8: Through quantitative PCR and TCID 50 Measure the liquid samples obtained before and after introducing the virus disinfection liquid to obtain the disinfection rate of influenza A H1N1 virus.

[0152] Example 2

[0153] As Figure 4 shown, this example uses the test system of the present invention to conduct an indoor secondary organic aerosol synthesis test under photoreaction conditions. The equipment used in this example is as follows:

[0154] ①Aerosol generation subsystem 10

[0155] The aerosol generation subsystem includes a gas generator 11, a pressure reducing valve 12, a first filter 13, a first gas mass flow controller 14, an aerosol production device 15, and a particle size screening device 16.

[0156] The gas generator 11 selects a compressed air gas cylinder.

[0157] The first filter 21 is a HEPA filter membrane type filter, which can filter particles with a diameter of more than 0.22 μm in the compressed air flowing out of the pressure reducing valve, effectively filtering the interference of the inlet gas on the subsequent test results.

[0158] The aerosol production device 15 selects a model 3076 aerosol generator produced by TSI Company of the United States. The constant flow atomizing aerosol generator is a Collison type atomizer. The standard aerosol flow rate is 3 L / min, and the particle size range for generating high-concentration aerosol is 0.01 - 2 μm.

[0159] The particle size screening device 16 selects a PM10 impact sampler from Dekati Company of Finland. With 2.5 μm as the screening target, aerosols with a size below 2.5 μm are screened into the rotary drum reaction chamber 62.

[0160] The gas in the compressed air cylinder flows through a pressure reducing valve and a HEPA membrane filter, and then enters the Model 3076 aerosol generator through the first gas mass flow controller to generate ammonium sulfate seed aerosol. Air with a flow rate of 3 L / min is controlled by the pressure reducing valve and the first gas mass flow controller simultaneously and enters the Model 3076 aerosol generator to generate ammonium sulfate seed aerosol.

[0161] ② Airflow regulation subsystem 20

[0162] The airflow regulation subsystem includes a membrane dryer 21, a temperature and humidity control device 22, a second filter 23, a third filter 24, a gas mass flowmeter 25, and a first air pump 26.

[0163] The temperature and humidity control device 22 selects the DHT-100 temperature and humidity chamber of Duohe Test Co., Ltd., with a volume of 100 L. Its temperature and humidity range is 0 - 120 °C, 5% - 98% relative humidity. The temperature fluctuation is ±0.1 - ±0.5 °C, the uniformity is ±0.5 - ±1.5 °C, and the humidity fluctuation is ±1.0% - ±2.0%.

[0164] Both the second filter 23 and the third filter 24 are HEPA membrane filters.

[0165] The gas mass flowmeter 25 selects a glass rotameter.

[0166] Regulate the airflow to enter the temperature and humidity control box after passing through the membrane dryer, flow through the HEPA membrane filter, and finally enter the test subsystem. The air pump enables the above-mentioned regulated airflow to enter the temperature and humidity of the test subsystem by pumping.

[0167] ③ Test subsystem 60

[0168] The test subsystem includes an ultraviolet light control module 61, a rotary drum reaction chamber 62, and a temperature control compartment 63.

[0169] The overall rotary drum reaction chamber 62 is made of aluminum alloy as the processing material, and the transmission method selects gear and roller transmission to keep the drum body rotating at a stable speed. The volume of the drum body is 1 m 3 . The rotary drum main body consists of a shell, an end cover, a shell connecting piece, a support shaft, and a central shaft. Multiple sealing rings are arranged radially, and a combination of a sealed bearing and an oil seal is adopted axially to ensure airtightness. The inner surface of the rotary drum is made of polytetrafluoroethylene material, and the outer surface of the polytetrafluoroethylene cylindrical tube is chemically treated and then fitted to the inner surface of the rotary drum. The rotary drum rotates at a speed of 1 - 3 rpm (the speed can be adjusted by the motor) to maintain the secondary organic aerosol in a suspended state.

[0170] The temperature control compartment 63 selects an electrothermal forced air thermostat from Huadong Instruments, with a maximum heating power of 1500W, a temperature control range from room temperature to 80°C, a temperature fluctuation of ±0.2°C, and a setting accuracy of 0.1°C.

[0171] ④ Test substance release subsystem

[0172] The test substance release subsystem includes a VOCs generation device and an oxidant release device. The VOCs generation device includes a zero air generator 41, a second gas mass flow controller 42, a second liquid storage tank 43, and a microsyringe 44.

[0173] The zero air generator selects the AADCO company model 737-5 zero air generator. The zero air generator outputs ozone, methane hydrocarbons, nitrogen oxides, hydrogen sulfide, carbonyl sulfide, carbon monoxide, carbon dioxide, and the concentration of fluorocarbons <0.5 ppb, and the output flow rate is 0 - 5 L / min.

[0174] The microsyringe selects a 10 μL injection syringe.

[0175] The zero air generated by the zero air generator flows through the second gas mass flow controller into the second liquid storage tank 43 to purge the volatile organic compound standard solution injected into the second liquid storage tank 43 by the microsyringe and flows into the test subsystem.

[0176] The oxidant release device includes an oxidant generator 51 and a third gas mass flow controller 52. The oxidant generator can generate oxidants such as ozone and hydrogen peroxide.

[0177] The ozone generator selects the American jelight 600, which can generate ozone with a concentration exceeding 6000 ppm by ultraviolet light, and the maximum flow rate setting is 0.5 L / min.

[0178] ⑤ Detection and monitoring subsystem 70

[0179] The detection and monitoring subsystem 70 includes a temperature and humidity sensor, an aerosol sampling instrument, an on-line measuring instrument, and a particle size testing device. The aerosol sampling instrument includes an aerosol sampler 75, a fourth filter 76, and a second air pump 77. The particle size testing device includes a particle size spectrometer 78 and a computer 79. The on-line measuring instrument includes a carbon monoxide gas measuring instrument 72, a sulfur dioxide gas measuring instrument 73, and an ozone measuring instrument 74.

[0180] The temperature and humidity sensor 71 selects the Vaisala company HMT-120 temperature and humidity transmitter, which is respectively set at the inlet and outlet of the rotary drum reaction chamber to measure the temperature and humidity inside the rotary drum in real time.

[0181] The secondary organic aerosol sampler 75 uses Whatman quartz filter membranes (90 mm in diameter, 0.45 μm in pore size) for sampling secondary organic aerosols, and the sampling flow rate is 10 L / min.

[0182] The second air pump 77 selects the SCJ-60 air flow pump of Shanghai Lichen Instrument Technology Co., Ltd., which has the advantages of small volume, light weight and good stability.

[0183] The fourth filter 76 selects a HEPA filter membrane type filter.

[0184] The particle size spectrometer 78 selects the SMPS scanning electrical mobility particle sizer of TSI Company, which can measure the number concentration and particle size distribution of secondary organic aerosols with an aerodynamic diameter in the range of 1-1000 nm in the aerosol. The differential mobility analyzer (DMAs) and six condensation particle counters (CPCs) can be selected for combined use. The particle size spectrometer is connected to the particle size spectrometer interface through a pipeline, and the particle size spectrometer is electrically connected to the computer.

[0185] The carbon monoxide gas detector 72 selects the carbon monoxide analyzer (48i) produced by Thermo Company of the United States to monitor the carbon monoxide gas concentration in the drum reaction chamber in real time online; the sulfur dioxide gas detector 73 selects the sulfur dioxide analyzer (43i) produced by Thermo Company of the United States to monitor the sulfur dioxide gas concentration in the drum reaction chamber in real time online; the ozone detector 74 selects the ozone online analyzer (49i) produced by Thermo Company of the United States to monitor the ozone concentration in the drum reaction chamber in real time online.

[0186] ⑥ Valve assembly

[0187] The first three-way valve 81, the second three-way valve 82 and the third three-way valve 85 are all three-way valves, and the three-way valves connect a passage and two branches with independent control switches.

[0188] The first four-way valve 83 and the second four-way valve 84 are all four-way valves, and the four-way valves connect a passage and three branches with independent control switches.

[0189] The following are the specific steps for the experiment at room temperature of 25 °C:

[0190] 1: Start the constant temperature and humidity box, set it to the set temperature of 25 °C and the relative humidity of 50%. Open the upper branch switch of the second four-way valve 84, turn on the first air pump, adjust the flow rate to 6 L / min, open the right branch switch of the first four-way valve 83 and the right branch switch of the first three-way valve 81, and use the zero air generated by the zero air generator to wash the drum reaction chamber 62, and then observe the readings of the temperature and humidity sensors to ensure they are correct.

[0191] 2: Open the lower branch switch of the first four-way valve 83, and at the same time close the right branch switch of the first four-way valve 83 and the right branch switch of the first three-way valve 81. Set the output speed of the drum frequency conversion motor to 60 rpm, and at this time the drum speed is 2 rpm.

[0192] 3: Open the upper branch switch of the first four-way valve 83, open the first mass flow controller and the compressed air cylinder, adjust the atomizing air flow rate to 6.1 L / min, continuously atomize the ammonium sulfate solution for 1 min to generate seed aerosol and introduce it into the drum reaction chamber, and then close the upper branch switch of the first four-way valve 83.

[0193] 4: At the same time, open the right branch switch of the first four-way valve 83 and the lower branch switch of the first three-way valve 81, turn on the ozone generator to generate ozone and inject it into the drum reaction chamber, and the mass flow meter controls the concentration entering the drum reaction chamber. The ozone concentration in the drum reaction chamber is about 50×10 -9 (V / V) ozone, seal the sampling port, and close the lower branch switch of the first three-way valve 81.

[0194] 5: At the same time, open the right branch switch of the first four-way valve 83 and the right branch switch of the first three-way valve 81, use a 10 μL injection syringe to inject 5 μL of 2% formaldehyde aqueous solution into the liquid storage tank, and the zero gas generator generates zero gas with a flow rate of 2 L / min to blow the volatile organic compound formaldehyde into the drum reaction chamber. Then, simultaneously close the upper branch switch of the third three-way valve 85, the right branch switch of the first four-way valve 83, and the right branch switch of the first three-way valve 81.

[0195] 6: All reactants are remixed for 5 min by rotating the drum, and then turn on the ultraviolet lamp for photo-oxidation reaction.

[0196] 7: During the reaction process, open the lower branch switch of the second four-way valve 84, and the carbon monoxide gas detector, carbon monoxide gas detector, and ozone detector monitor the concentration changes of carbon monoxide gas, carbon monoxide gas, and ozone in the drum reaction chamber in real time. After the reaction is completed, open the left branch switch of the second four-way valve 84 and the lower branch switch of the third three-way valve 85, and use a quartz filter membrane for particulate sampling. The sampling flow rate is 10 L / min, and the sampling time is 10 min. Pass a hot N2 gas flow at a certain temperature through the sampling filter membrane to convert the particulate phase products on the quartz filter membrane into gas phase components, and directly enter the mass spectrometer for analysis to determine the composition of the particulate phase products of the secondary organic aerosol. At the same time, open the left branch switch of the second four-way valve 84 and the left branch switch of the third three-way valve 85 (keep the lower branch switch of the third three-way valve 85 in the open state, and conduct particulate sampling and particle size monitoring simultaneously), turn on the SMPS scanning mobility particle sizer to measure the number concentration and particle size distribution of the particulate matter of the secondary organic aerosol after the reaction, and the yield of the secondary organic aerosol can be calculated. After the detection is completed, close all valves.

[0197] In summary, the test system of the present invention can be used to determine the effects of different types and proportions of disinfection liquids and disinfection time on the survival rates of virus and bacterial pathogen aerosols, can determine the effects of ultraviolet light intensity and irradiation time on the survival rates of virus and bacterial pathogen aerosols, and can also determine the effects of related factors such as inorganic gaseous precursor concentration, oxidant type and concentration, and ultraviolet light intensity on the synthetic components, particle size distribution, and concentration of indoor secondary organic aerosols.

[0198] The above description of the embodiments is for the convenience of those of ordinary skill in the art to understand and use the present invention. However, the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, making equivalent substitutions or changes, should be covered within the protection scope of the present invention.

Claims

1. A multifunctional indoor aerosol test system, characterized in that, Comprising: A test subsystem (60), including a drum reaction chamber (62); An aerosol generation subsystem (10) for inputting aerosol into the test subsystem (60); An air flow regulation subsystem (20), including an inlet-side regulation module and an outlet-side regulation module; A test substance release subsystem, including a disinfection liquid release device (30), a volatile organic compound generation device (40), and an oxidant release device (50), for inputting test substances into the test subsystem (60), where the test substances include one or more of disinfection liquid, volatile organic compounds, and oxidants; A detection and monitoring subsystem (70) for detecting and monitoring the changes in the test subsystem in real time, including an on-line measuring instrument, an aerosol sampling instrument, and a particle size testing device.

2. The multifunctional indoor aerosol test system according to claim 1, characterized in that: The indoor aerosol test system further includes a first three-way valve (81), a third three-way valve (85), a first four-way valve (83), and a second four-way valve (84); the outlets of the volatile organic compound generation device (40) and the oxidant release device (50) are respectively connected to the first valve port and the second valve port of the first three-way valve (81), the third valve port of the first three-way valve (81), the outlet of the aerosol generation subsystem (10), and the outlet of the inlet-side regulation module are respectively connected to the first valve port, the second valve port, and the third valve port of the first four-way valve (83), and the fourth valve port of the first four-way valve (83) is connected to the inlet of the drum reaction chamber (62); the outlet of the drum reaction chamber (62) is connected to the first valve port of the second four-way valve (84), and the second valve port, the third valve port, and the fourth valve port of the second four-way valve (84) are respectively connected to the inlet of the outlet-side regulation module, the inlet of the on-line measuring instrument, and the first valve port of the third three-way valve (85), and the second valve port and the third valve port of the third three-way valve (85) are respectively connected to the inlet of the aerosol sampling instrument and the inlet of the particle size testing device.

3. The multifunctional indoor aerosol test system according to claim 1 or 2, wherein: The indoor aerosol test system further includes a second three-way valve (82); the disinfection liquid release device (30) includes a first liquid storage tank (31), a liquid delivery pump (32), a liquid flow meter (33), and two disinfection liquid generation devices (34), and the two disinfection liquid generation devices (34) are respectively installed at both ends of the drum reaction chamber (62). The first liquid storage tank (31) stores disinfection liquid, the first liquid storage tank (31) is connected to the inlet of the liquid delivery pump (32), the outlet of the liquid delivery pump (32) is connected to the inlets of the two disinfection liquid generation devices (34) through the second three-way valve (82), the outlets of the two disinfection liquid generation devices (34) are both connected to the inside of the drum reaction chamber (62), and a liquid flow meter (33) is provided on the pipeline between the outlet of the liquid delivery pump (32) and the second three-way valve (82).

4. The multifunctional indoor aerosol test system according to claim 2, wherein: The volatile organic compound generating device (40) includes a zero air generator (41), a second gas mass flow controller (42), a second liquid reservoir (43), and a syringe (44). The syringe (44) is vertically arranged directly above the second liquid reservoir (43). On both sides of the outlet end of the syringe (44), a zero air delivery pipeline and an organic matter delivery pipeline are arranged respectively. The outlet of the zero air delivery pipeline and the inlet of the organic matter delivery pipeline are arranged opposite to each other. The inlet of the zero air delivery pipeline is communicated with the outlet of the zero air generator (41). A second gas mass flow controller (42) is arranged on the zero air delivery pipeline. The outlet of the organic matter delivery pipeline is communicated with the first valve port of the first three-way valve (81). The oxidant releasing device (50) includes an oxidant generator (51) and a third gas mass flow controller (52) connected in sequence. The outlet of the third gas mass flow controller (52) is communicated with the second valve port of the first three-way valve (81).

5. The multifunctional indoor aerosol test system according to claim 2, characterized in that: The on-line measuring instrument includes a carbon monoxide gas measuring instrument (72), a sulfur dioxide gas measuring instrument (73), and an ozone measuring instrument (74). The inlets of the carbon monoxide gas measuring instrument (72), the sulfur dioxide gas measuring instrument (73), and the ozone measuring instrument (74) are connected in parallel and then communicated with the third valve port of the second four-way valve (84). The aerosol sampling instrument includes an aerosol sampler (75), a fourth filter (76), and a second air pump (77) connected in sequence. The inlet of the aerosol sampler (75) is communicated with the second valve port of the third three-way valve (85). The particle size testing device includes a particle size spectrometer (78) and a computer (79). The inlet of the particle size spectrometer (78) is communicated with the third valve port of the third three-way valve (85). The particle size spectrometer (78) is electrically connected to the computer (79).

6. The multifunctional indoor aerosol test system according to claim 5, wherein: The aerosol sampler (75) includes a solid sampler, a liquid sampler, or a membrane sampler.

7. The multifunctional indoor aerosol test system according to claim 2, wherein: The aerosol generating subsystem (10) includes a gas generator (11), a pressure reducing valve (12), a first filter (13), a first gas mass flow controller (14), an aerosol production device (15), and a particle size screening device (16) connected in series. The outlet of the particle size screening device (16) is communicated with the second valve port of the first four-way valve (83). The inlet side regulation module of the air flow regulation subsystem includes a membrane dryer (21), a constant temperature and humidity control device (22), and a second filter (23) connected in series. The outlet of the second filter (23) is communicated with the first valve port of the first four-way valve (83). The outlet side regulation module of the air flow regulation subsystem includes a third filter (24), an air quality flowmeter (25), and a first air pump (26) connected in series. The inlet of the third filter (24) is communicated with the second valve port of the second four-way valve (84).

8. The multifunctional indoor aerosol test system according to claim 1, characterized in that: The test subsystem further includes an ultraviolet light control module (61) and a temperature control compartment (63). The temperature control compartment (63) is arranged outside the drum reaction chamber (62), and a temperature and humidity sensor (71) is arranged at each of the inlet and outlet of the drum reaction chamber (62).

9. A method for disinfecting and killing pathogen aerosols using the multifunctional indoor aerosol test system according to any one of claims 1 to 8, characterized in that, It includes the following steps: S1. Connect the first valve port of the second four-way valve (84) to the second valve port, and connect the second valve port and the third valve port of the first four-way valve (83) to the fourth valve port. Generate virus aerosol by using the aerosol generation subsystem (10) and introduce it into the drum reaction chamber (62). S2. After the ventilation ends, shut off the second valve port of the second four-way valve (84) and the second valve port and the third valve port of the first four-way valve (83). S3. Connect the third valve port of the first four-way valve (83) to the fourth valve port, and connect the second valve port of the third three-way valve (85) to the first valve port. Perform initial aerosol sampling by using an aerosol sampling instrument. After the aerosol sampling ends, shut off the second valve port of the third three-way valve (85) and open the third valve port. Perform initial particle size testing by using a particle size testing device. After the particle size testing ends, shut off the third valve port of the third three-way valve (85) and the third valve port of the first four-way valve (83). S4. Connect the three valve ports of the second three-way valve (82). Generate virus disinfectant liquid by using the disinfectant liquid release device (30) and introduce it into the drum reaction chamber (62). After the disinfection ends, close the second three-way valve (82). S5. Perform post-disinfection aerosol sampling and post-disinfection particle size testing according to step S3. S6. Analyze the results of the initial aerosol sampling and particle size testing, and the post-disinfection aerosol sampling and particle size testing to obtain the pathogen aerosol disinfection test results.

10. A method for testing secondary organic aerosol using the multifunctional indoor aerosol test system according to any one of claims 1 to 8, characterized in that, It includes the following steps: S1. Connect the first valve port of the second four-way valve (84) to the second valve port, connect the first valve port of the first four-way valve (83) to the fourth valve port, connect the first valve port of the first three-way valve (81) to the third valve port, and open the valve of the first air pump (26). Clean the drum reaction chamber (62) by using the zero gas generator (41) in the volatile organic compound generation device (40). S2. After the cleaning ends, close the first valve port and the third valve port of the first three-way valve (81). S3. At the same time, connect the second valve port and the third valve port of the first four-way valve (83) to the fourth valve port. Generate virus aerosol by using the aerosol generation subsystem (10) and introduce it into the drum reaction chamber (62). After the ventilation ends, shut off the second valve port of the first four-way valve (83). S4. Connect the first valve port and the third valve port of the first four-way valve (83) to the fourth valve port, and connect the second valve port of the first three-way valve (81) to the third valve port. Introduce an oxidant into the drum reaction chamber (62) by using the oxidant release device (50). After the ventilation ends, shut off the second valve port of the first three-way valve (81). S5. Connect both the first valve port and the third valve port of the first four-way valve (83) to the fourth valve port, connect the first valve port of the first three-way valve (81) to the third valve port, and introduce the gas containing volatile organic compounds into the rotary drum reaction chamber (62) using the volatile organic compound generating device (40). After the gas supply ends, simultaneously shut off the second valve port of the second four-way valve (84), the first valve port of the first four-way valve (83), and the first valve port of the first three-way valve (81). S6. Mix all reactants in the rotary drum reaction chamber (62), and turn on the ultraviolet light control module (61) to carry out the photo-oxidation reaction. During the reaction process, connect the third valve port of the second four-way valve (84) to the first valve port to detect the concentration changes of carbon monoxide, sulfur dioxide, and ozone in the rotary drum reaction chamber (62) in real time. S7. After the photo-oxidation reaction ends, connect the fourth valve port of the second four-way valve (84) to the first valve port, and connect the second valve port of the third three-way valve (85) to the first valve port, and perform aerosol sampling using the aerosol sampling instrument. After the aerosol sampling ends, shut off the second valve port of the third three-way valve (85) and open the third valve port, and perform the initial particle size test using the particle size testing device. After the particle size test ends, shut off the third valve port of the third three-way valve (85) and the third valve port of the first four-way valve (83). S8. Analyze the aerosol sampling results and the particle size test results to obtain the test results of secondary organic aerosols.

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