Immune cell detector and detection method thereof

Abstract

The invention relates to the technical field of cell detection, and discloses an immune cell detector and a detection method.The immune cell detector comprises a reaction disc, a plurality of clamping frames are arranged on the reaction disc in a circumferential array mode, reaction cups are placed in the clamping frames, a surrounding plate is coaxially installed on the outer side of the reaction disc, and an air cylinder is arranged in the detection direction of the surrounding plate; a detection clamping plate is fixedly installed at the telescopic end of the air cylinder, a car frame is rotationally installed on the surrounding plate, the detection clamping plate and the car frame are intermittently clamped, a machine shell is arranged in the center of the reaction disc, a hydraulic cylinder is installed on the machine shell, a driving clamping plate is arranged at the telescopic end of the hydraulic cylinder and corresponds to the detection clamping plate, and a beating plate is rotationally arranged on the machine shell. A beating assembly is further arranged on the machine shell. According to the scheme, different stages can be detected, transferring and clamping of the reaction cup are automatically achieved, detection and automatic discharging are carried out in the stable state, and detection is more automatic.

Description

Technical Field

[0001] The present invention relates to the technical field of cell detection, and specifically, to an immune cell detector and a detection method thereof. Background Art

[0002] In the related art, immune function detection mainly evaluates the function of the immune system by detecting relevant indicators in the blood. These indicators include white blood cell count, lymphocyte count, and levels of various immunoglobulins, etc. During the test, a reaction cup is used to hold the reaction reagent and the body fluid sample and mix them. Subsequently, the reaction cup reacts in a heated and insulated environment for a period of time, and then is transported to the detection position for photoelectric conversion detection.

[0003] In an immunoanalyzer, disposable reaction cups, as test consumables, need to be replenished and removed. They are usually placed in the reaction cup accommodation grooves distributed circumferentially on the reaction disk. During the luminescence detection, the reaction cup to be measured needs to be automatically moved to the detection area and then automatically moved away after the detection is completed. Since chemiluminescence relies on the PMT to accurately capture transient light signals at a fixed position, the luminescence detection has high requirements for the position accuracy of the reaction cup. Therefore, it is necessary to better achieve the positioning, stable detection, and automatic blanking of the reaction cup. Therefore, we propose an immune cell detector and a detection method thereof. Summary of the Invention

[0004] The present invention provides an immune cell detector and a detection method thereof. The immune cell detector and the detection method thereof can detect different stages, automatically realize the transfer, clamping, detection under stable state, and automatic blanking of the reaction cup, making the detection more automated, and solving the problem mentioned in the above background art that it is necessary to better achieve the positioning, stable detection, and automatic blanking of the reaction cup.

[0005] To achieve the above object, the present disclosure provides an immune cell detector, including a reaction disk, a plurality of clamping frames are arranged in a circumferential array on the reaction disk, reaction cups are placed in the clamping frames, a surrounding plate is coaxially installed outside the reaction disk, a cylinder is arranged in the detection direction of the surrounding plate, a detection clamping plate is fixedly installed at the telescopic end of the cylinder, a sedan frame is rotatably installed on the surrounding plate, the detection clamping plate is intermittently engaged with the sedan frame, a machine housing is arranged at the center of the reaction disk, a hydraulic cylinder is installed on the machine housing, a driving clamping plate is arranged at the telescopic end of the hydraulic cylinder, the driving clamping plate is arranged corresponding to the detection clamping plate, a striking plate is rotatably arranged on the machine housing, and a striking component is further arranged on the machine housing. When the driving clamping plate and the detection clamping plate clamp the reaction cup until the detection is completed, the striking plate drives the cylinder to operate through the striking component, realizing the separation of the detection clamping plate and the driving clamping plate.

[0006] Optionally, a number of hollow parts are arranged in a circumferential array on the reaction disc, the clamping frame is hinged in the hollow part, and the clamping frame is arranged as an arc-shaped clamping hand with a silica gel head at the clamping end.

[0007] Optionally, a fixed sleeve is installed on the side of the active clamping plate, a elastic sleeve is fixedly installed at the telescopic end of the hydraulic cylinder, the elastic sleeve is inserted and engaged with the fixed sleeve, and the elastic sleeve is arranged as a sleeve with a spring inside.

[0008] Optionally, a synchronous rod is installed on the side of the fixed sleeve, a reinforcement seat is installed on the side of the machine shell, the striking plate is hinged on the reinforcement seat, a guiding through groove is opened on the striking plate, and the synchronous rod passes through the guiding through groove; The guiding through groove includes a horizontal through groove and an arc through groove, and the arc through groove is connected to the side of the horizontal through groove adjacent to the reaction cup; In the normal state, the synchronous rod is located in the horizontal through groove; During detection, the synchronous rod is located in the arc through groove.

[0009] Optionally, a piston cylinder is installed on the machine shell, a return air pipe and an exhaust pipe are connected to the side of the piston cylinder, the return air pipe and the exhaust pipe are connected to the cylinder, and the striking plate is intermittently driven and connected to the piston cylinder.

[0010] Optionally, the striking assembly includes a fixed rack fixedly installed on the machine shell, a struck block slidably installed in the machine shell, and a piston plate working in the piston cylinder. A double-tooth rack is slidably arranged on the side of the fixed rack, a connecting block is installed on the side of the struck block, a distance-increasing double gear is rotatably installed on the side of the connecting block, the distance-increasing double gear is simultaneously meshed with the double-tooth rack and the fixed rack, a power output rack is installed on the side of the piston plate, a transmission gear is rotatably installed inside the machine shell, the transmission gear is simultaneously meshed with the power output rack and the double-tooth rack, and a return spring for resetting is installed between the struck block and the machine shell.

[0011] Optionally, a fixed frame is arranged in the detection direction of the reaction disc, the cylinder is fixedly installed on the fixed frame, a blanking frame is fixedly installed in the blanking direction of the fixed frame, and the blanking frame is intermittently butted with the sedan frame; An output wheel shaft is rotatably installed at the end of the synchronous rod, the output wheel shaft is in clearance fit with the horizontal through groove, a shaft end wheel is fixedly installed at the end of the output wheel shaft, and a return rod is installed on the side of the shaft end wheel; An L-shaped groove is opened on the machine shell, a return plate is slidably installed in the L-shaped groove, an L-shaped rod is vertically connected to the return plate, the return plate is intermittently matched with the return rod, and the L-shaped rod is in transmission connection with the sedan frame.

[0012] Optionally, a blanking shaft is rotatably mounted on the enclosure, the car frame is fixedly mounted on the blanking shaft, a driving rod is rotatably mounted on the casing, a pulley set is commonly mounted between the blanking shaft and the driving rod, a gear roller is coaxially mounted on the blanking shaft, a transverse rack is connected to the end of the L-shaped rod, and the transverse rack is engaged with the gear roller; When the reversing plate rotates and drives the reversing rod to move, the car frame rotates and docks with the unloading rack.

[0013] Optionally, a pressure spring is installed between the reversing plate and the L-shaped groove wall, a corresponding notch is provided at one end of the car frame adjacent to the detection clamping plate, and a docking notch is provided at the corresponding end of the detection clamping plate.

[0014] According to a second aspect of the present disclosure, a method for detecting immune cells is provided, using the immune cell detector as described above, comprising the following specific steps: S1. Sample Collection and Pretreatment: Collect biological samples and pre-treat them by filtering them through a 5-10 micron pore size filter to remove impurities and large cell aggregates. The filtered samples are then centrifuged at 1500-2000 rpm for 5-10 minutes to separate the supernatant containing immune cells. S2. Immune cell labeling: Add specific immune cell markers to the supernatant, including fluorescein-labeled antibodies and magnetic bead-labeled antibodies. Incubate the supernatant with the markers at 37°C for 15-30 minutes to allow the markers to fully bind to the immune cells. S3. The incubated sample is treated with a magnetic field, causing the magnetic bead-labeled immune cells to be adsorbed on one side of the magnetic field, thereby separating them from other components. The immune cells adsorbed on one side of the magnetic field are then eluted with an eluent, thereby enriching the immune cells. S4. Place the enriched immune cell sample in a reaction cup and place it on a reaction plate. Use excitation light of different wavelengths to stimulate the fluorescein-labeled antibodies on the immune cells, detect the fluorescent signals emitted by the immune cells, and transmit the acquired fluorescent signal data to the data processing system. The data processing system uses a deep learning-based algorithm model to process and analyze the data, and generates an immune cell analysis and detection report based on the analysis results. The report content includes information such as the type, number, proportion, and various characteristic parameters of the immune cells.

[0015] Through the above technical solution, when the immune cell detector and its detection method provided by the present disclosure are in use: through the setting of the synchronization rod, during the operation of the hydraulic cylinder, the synchronization rod moves in the guiding slot, thereby driving the striking plate to rotate, thus triggering the striking assembly. By using the transmission of the striking assembly to trigger the piston cylinder and the air cylinder, when the detection is completed, the synchronization rod is located at the peak of the arc-shaped slot. At this time, the striking assembly does the most work, causing the detection clamping plate to be driven upward by the air cylinder, thereby realizing the separation of the detection clamping plate from the active clamping plate. At this time, the detection has ended, the reaction cup loses the support of the detection clamping plate, and the spring in the elastic sleeve is released, so that the reaction cup is pushed down by the active clamping plate to achieve automatic blanking; Through the cooperation of the synchronization rod with the striking plate and the guiding slot, this solution can detect different stages, automatically realize the transfer, clamping, detection under stable conditions and automatic blanking of the reaction cup, making the detection more automated, ensuring the detection accuracy, and improving the immune cell detection efficiency.

[0016] Other features and advantages of the present disclosure will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The drawings are used to provide a further understanding of the present disclosure, and constitute a part of the specification. Together with the following specific implementation, they are used to explain the present disclosure, but do not constitute a limitation to the present disclosure. In the drawings: Figure 1 It is a schematic perspective view of the overall structure of the present invention before detection.

[0018] Figure 2 For the present invention Figure 1 Enlarged structural schematic diagram of part A.

[0019] Figure 3 It is a schematic perspective view of the overall structure of the present invention when the detection is completed.

[0020] Figure 4 For the present invention Figure 3 Enlarged structural schematic diagram of part B.

[0021] Figure 5 It is an exploded structural schematic diagram of the overall parts of the present invention.

[0022] Figure 6 It is a schematic diagram of the structure of the active clamping plate and the striking assembly of the present invention.

[0023] Figure 7 It is a schematic cross-sectional view from above when the reaction cup is separated from the clamping rack in the tray of the present invention.

[0024] Figure 8 It is a schematic diagram of the structure of the guiding slot of the present invention.

[0025] Figure 9 Schematic diagram of the overall side view cross-section structure of the present invention.

[0026] Figure 10 Schematic diagram of the three-dimensional structure of the L-shaped rod and the bridge frame of the present invention.

[0027] Figure 11 Schematic diagram of the movement of the striking component of the present invention.

[0028] Figure 12 Schematic diagram of the movement structure of the L-shaped rod of the present invention.

[0029] Description of reference numerals: 10, reaction cup; 110, reaction disc; 120, enclosure; 130, hollow part; 140, clamping bracket; 20, housing; 210, hydraulic cylinder; 220, active clamping plate; 230, fixed sleeve; 240, elastic sleeve; 260, synchronous rod; 270, output wheel shaft; 280, shaft end wheel; 290, return rod; 310, reinforcement base; 320, striking plate; 330, guiding through slot; 331, horizontal through slot; 332, arc through slot; 410, struck block; 420, connecting block; 430, distance-increasing double gear; 440, fixed rack; 450, double-tooth rack; 460, driving gear; 470, output rack; 480, return spring; 490, piston plate; 510, return plate; 520, L-shaped rod; 530, transverse rack; 540, pressure spring; 550, L-shaped groove; 560, driving rod; 570, gear roller; 580, pulley set; 590, blanking shaft; 610, detection clamping plate; 611, docking notch; 620, cylinder; 630, fixed frame; 640, bridge frame; 641, corresponding notch; 650, blanking frame; 710, piston cylinder; 720, return air pipe; 730, exhaust pipe. Detailed implementation manners

[0030] In order to make the above objects, features and advantages of the present disclosure more obvious and understandable, the following detailed description of the specific implementation manners of the present disclosure will be given with reference to the accompanying drawings. Many specific details are set forth in the following description in order to fully understand the present disclosure. However, the present disclosure can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present disclosure. Therefore, the present disclosure is not limited by the specific embodiments disclosed below.

[0031] In the description of the present disclosure, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. These are only for the convenience of describing the present disclosure and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present disclosure. The terms "first" and "second" used are for distinguishing one element from another and do not have sequentiality and importance. In addition, when the following description involves the drawings, the same reference numerals in different drawings represent the same or similar elements, and the present disclosure will not elaborate on this here.

[0032] In the present disclosure, unless otherwise clearly specified and defined, the terms "mounted", "connected", "joined", "fixed", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements or the interaction relationship between two elements, unless otherwise clearly defined. For those of ordinary skill in the art, the specific meanings of the above terms in the present disclosure can be understood according to specific circumstances.

[0033] According to some embodiments of the present disclosure, an immune cell detector and its detection method are provided. As shown in Figures 1 - 12 the immune cell detector includes a reaction disk 110. A plurality of clamping brackets 140 are arranged in a circumferential array on the reaction disk 110. Reaction cups 10 are placed in the clamping brackets 140. A surrounding plate 120 is coaxially installed outside the reaction disk 110. A cylinder 620 is arranged in the detection direction of the surrounding plate 120. A detection clamping plate 610 is fixedly installed at the telescopic end of the cylinder 620. A lifting frame 640 is rotatably installed on the surrounding plate 120. The detection clamping plate 610 is intermittently engaged with the lifting frame 640. A machine housing 20 is arranged at the center of the reaction disk 110. A hydraulic cylinder 210 is installed on the machine housing 20. A driving clamping plate 220 is arranged at the telescopic end of the hydraulic cylinder 210. The driving clamping plate 220 is arranged corresponding to the detection clamping plate 610. A striking plate 320 is rotatably arranged on the machine housing 20. A striking assembly is also arranged on the machine housing 20. When the driving clamping plate 220 and the detection clamping plate 610 clamp the reaction cup 10 until the detection is completed, the striking plate 320 drives the cylinder 620 to operate through the striking assembly, realizing the separation of the detection clamping plate 610 and the driving clamping plate 220.

[0034] In addition, a number of hollow portions 130 are arranged in a circumferential array on the reaction plate 110. The clamping frame 140 is hinged in the hollow portion 130, and the clamping frame 140 is set as an arc-shaped clamping hand with a silica gel head at the clamping end. A torsion spring is installed between the clamping frame 140 and the reaction plate 110 to facilitate the reset of the clamping frame 140.

[0035] Furthermore, a fixed sleeve 230 is installed on the side of the active clamping plate 220. A resilient sleeve 240 is fixedly installed at the telescopic end of the hydraulic cylinder 210. The resilient sleeve 240 is inserted and engaged with the fixed sleeve 230, and the resilient sleeve 240 is set as a sleeve with a spring inside.

[0036] A synchronous rod 260 is installed on the side of the fixed sleeve 230. A reinforcement seat 310 is installed on the side of the housing 20. The striking plate 320 is hingedly installed on the reinforcement seat 310. A guiding through groove 330 is formed on the striking plate 320, and the synchronous rod 260 passes through the guiding through groove 330. A torsion spring is installed at the hinge between the reinforcement seat 310 and the striking plate 320 to facilitate the automatic reset of the striking plate 320.

[0037] See Figure 8 , the guiding through groove 330 includes a horizontal through groove 331 and an arc-shaped through groove 332. The arc-shaped through groove 332 is connected to the side of the horizontal through groove 331 adjacent to the reaction cup 10; in the normal state, the synchronous rod 260 is located in the horizontal through groove 331; during detection, the synchronous rod 260 is located in the arc-shaped through groove 332.

[0038] In this way, through the cooperation of the fixed sleeve 230 and the resilient sleeve 240, when the hydraulic cylinder 210 operates, the active clamping plate 220 moves towards the reaction cup 10 and contacts it. The hydraulic cylinder 210 continues to extend, and the active clamping plate 220 pushes the reaction cup 10 towards the detection clamping plate 610, so that the reaction cup 10 is gradually clamped by the active clamping plate 220 and the detection clamping plate 610, and during this process, the reaction cup 10 gradually disengages from the clamping of the clamping frame 140. When the active clamping plate 220 and the detection clamping plate 610 completely clamp the reaction cup 10, the reaction cup 10 is already in the detection area and can be detected. At the same time, the hydraulic cylinder 210 continues to operate, and the extension amount is absorbed by the resilient sleeve 240. Through the setting of the synchronous rod 260, during the operation of the hydraulic cylinder 210, the synchronous rod 260 moves in the guiding through groove 330, thereby driving the striking plate 320 to rotate, thus triggering the striking assembly.

[0039] Specifically, a piston cylinder 710 is installed on the housing 20. A return air pipe 720 and an exhaust pipe 730 are connected to the side of the piston cylinder 710. The return air pipe 720 and the exhaust pipe 730 are connected to the air cylinder 620, and the striking plate 320 is intermittently drivingly connected to the piston cylinder 710.

[0040] See Figure 6, the striking assembly includes a fixed rack 440 fixedly installed on the casing 20, a struck block 410 slidably installed in the casing 20, and a piston plate 490 that does work in the piston cylinder 710. A double-toothed rack 450 is slidably arranged on the side of the fixed rack 440. A connecting block 420 is installed on the side of the struck block 410. A distance-increasing double gear 430 is rotatably installed on the side of the connecting block 420. The distance-increasing double gear 430 meshes with both the double-toothed rack 450 and the fixed rack 440 at the same time. An output rack 470 is installed on the side of the piston plate 490. A transmission gear 460 is rotatably installed inside the casing 20. The transmission gear 460 meshes with both the output rack 470 and the double-toothed rack 450 at the same time. A return spring 480 for resetting is installed between the struck block 410 and the casing 20.

[0041] When the struck block 410 moves under the action of an external force, through the transmission of the connecting block 420 and the distance-increasing double gear 430, the double-toothed rack 450 will move along the direction of the fixed rack 440. Due to the rolling stroke of the distance-increasing double gear 430, the actual moving distance of the double-toothed rack 450 will be greater than the moving distance of the struck block 410. This amplified moving distance is transmitted to the output rack 470 through the transmission gear 460, and then drives the piston plate 490 to move.

[0042] Through the above technical solution, when the immune cell detector provided by the present disclosure is in use, the piston cylinder 710 and the air cylinder 620 are triggered by the transmission of the striking assembly, so that when the detection is completed, the synchronous rod 260 is located at the peak of the arc-shaped through groove 332. At this time, the striking assembly does the most work, so that the detection clamping plate 610 is driven by the air cylinder 620 to move upward, thereby realizing the separation of the detection clamping plate 610 from the active clamping plate 220. At this time, the detection has ended, and the reaction cup 10 loses the support of the detection clamping plate 610, and the spring in the elastic sleeve 240 is released, so that the reaction cup 10 is pushed down by the active clamping plate 220 to realize automatic blanking; The air cylinder 620 is triggered only when the synchronous rod 260 reaches the peak of the arc-shaped through groove 332, ensuring the absolute stability of the clamping state during the detection process and avoiding the detection interruption caused by premature separation; Through the cooperation of the synchronous rod 260 with the striking plate 320 and the guiding through groove 330, this solution can detect different stages, automatically realize the transfer, clamping, detection under stable state and automatic blanking of the reaction cup 10, making the detection more automated, ensuring the detection accuracy, and improving the immune cell detection efficiency.

[0043] It should be noted that this embodiment also proposes an immune cell detection method, using the above immune cell detector, including the following specific steps: S1. Sample collection and pretreatment: Collect biological samples and perform pretreatment on the collected biological samples. First, remove impurities and large cell clumps in the samples through a filtration device, and the filtration device uses a filter screen with a pore size of 5-10 microns; then place the filtered samples in a centrifuge and centrifuge at a speed of 1500-2000 revolutions per minute for 5-10 minutes to separate the supernatant containing immune cells; S2. Immunocyte labeling: Add specific immunocyte markers to the supernatant. The specific immunocyte markers include fluorescein-labeled antibodies and magnetic bead-labeled antibodies. Incubate the supernatant with the added markers in a constant temperature environment at 37°C for 15-30 minutes to allow the markers to fully bind to the immune cells; S3. Process the incubated samples using a magnetic field so that the immune cells labeled with magnetic beads are adsorbed on one side of the magnetic field, thereby separating them from other components. Elute the immune cells adsorbed on one side of the magnetic field with an eluent to achieve the enrichment of immune cells; S4. Place the enriched immune cell samples in reaction cup 10 and place them on reaction tray 110. Use excitation light of different wavelengths to excite the fluorescein-labeled antibodies on the immune cells, detect the fluorescence signals emitted by the immune cells, and transmit the obtained fluorescence signal data to a data processing system. The data processing system uses an algorithm model based on deep learning to process and analyze the data, and generates an immune cell analysis and detection report according to the analysis results. The report content includes information such as the type, quantity, proportion, and various characteristic parameters of the immune cells.

[0044] In some embodiments of the present disclosure, as shown in Figures 1 - 12 a fixed frame 630 is provided in the detection direction of reaction tray 110. A cylinder 620 is fixedly installed on fixed frame 630. A blanking frame 650 is fixedly installed in the blanking direction of fixed frame 630, and blanking frame 650 is intermittently docked with carriage 640.

[0045] An output wheel shaft 270 is rotatably installed at the end of synchronous rod 260. Output wheel shaft 270 is in clearance fit with horizontal through slot 331. A shaft end wheel 280 is fixedly installed at the end of output wheel shaft 270, and a return rod 290 is installed on the side of shaft end wheel 280.

[0046] An L-shaped slot 550 is provided on the housing 20. A return plate 510 is slidably installed in L-shaped slot 550. Return plate 510 is vertically connected to an L-shaped rod 520. Return plate 510 is intermittently engaged with return rod 290, and L-shaped rod 520 is drivingly connected to carriage 640.

[0047] Among them, a blanking shaft 590 is rotatably installed on the enclosing plate 120, a car frame 640 is fixedly installed on the blanking shaft 590, a driving rod 560 is rotatably installed on the machine shell 20, a pulley group 580 is jointly installed between the blanking shaft 590 and the driving rod 560, a gear roller 570 is coaxially installed on the blanking shaft 590, a transverse rack 530 is connected to the end of the L-shaped rod 520, and the transverse rack 530 meshes with the gear roller 570; When the return plate 510 rotates and toggles the return rod 290 to move, the car frame 640 rotates and docks with the blanking frame 650.

[0048] Specifically, a pressure spring 540 is installed between the return plate 510 and the wall of the L-shaped groove 550. One end of the car frame 640 adjacent to the detection clamping plate 610 is provided with a corresponding notch 641, and the corresponding end of the detection clamping plate 610 is provided with a docking notch 611.

[0049] It should be noted that the pulley group 580 is set as a combination of a conventional transmission wheel and a belt. The two transmission wheels are respectively installed on the blanking shaft 590 and the driving rod 560, and the belt is sleeved outside the two transmission wheels.

[0050] Through the above technical solutions, when the immune cell detector provided by the present disclosure is in use, through the cooperation of the car frame 640 and the detection clamping plate 610, the corresponding notch 641 is docked with the docking notch 611, making the clamping of the reaction cup 10 more stable. And when the synchronous rod 260 moves to the peak of the arc-shaped through groove 332, the detection has ended. At this time, the striking assembly is triggered, the detection clamping plate 610 moves upward and leaves the car frame 640, and through the cooperation of the return rod 290 and the return plate 510, after the synchronous rod 260 passes through the peak of the arc-shaped through groove 332, the return rod 290 rotates and lands on the return plate 510 and drives the return plate 510 to move, so that the transverse rack 530 drives the gear roller 570 to rotate. After a series of transmissions, the rotation of the car frame 640 is realized. Originally, the car frame 640 was horizontal and docked with the detection clamping plate 610. After the detection clamping plate 610 leaves, the car frame 640 rotates and changes to dock with the blanking frame 650. At this time, the reaction cup 10 is pushed by the active clamping plate 220 and slides down along the blanking frame 650 for blanking, which buffers the sliding of the reaction cup 10 during blanking and avoids damage to the cup body of the reaction cup 10 caused by direct pushing down. The blanking frame 650 is convenient for docking with an external automation device for other processing.

[0051] The preferred embodiments of the present disclosure have been described in detail above with reference to the accompanying drawings. However, the present disclosure is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present disclosure, various simple modifications can be made to the technical solutions of the present disclosure, and these simple modifications all belong to the protection scope of the present disclosure.

[0052] In addition, it should be noted that, in the above specific embodiments, the various specific technical features described can be combined in any suitable manner without conflict. To avoid unnecessary repetition, the present disclosure will not separately describe various possible combination manners.

[0053] Furthermore, any combinations can be made among the various different embodiments of the present disclosure, as long as they do not violate the idea of the present disclosure, and they should also be regarded as the content disclosed by the present disclosure.

Claims

1. An immune cell detector, comprising a reaction disk (110), wherein a plurality of clamping frames (140) are arranged in a circumferential array on the reaction disk (110), and reaction cups (10) are placed in the clamping frames (140), and characterized in that: A baffle plate (120) is coaxially installed outside the reaction plate (110). A cylinder (620) is arranged in the detection direction of the baffle plate (120). A detection clamping plate (610) is fixedly installed at the telescopic end of the cylinder (620). A car frame (640) is rotatably installed on the baffle plate (120). The detection clamping plate (610) is intermittently engaged with the car frame (640). A machine shell (20) is arranged at the center of the reaction plate (110). A hydraulic cylinder (210) is installed on the machine shell (20). A driving clamping plate (220) is arranged at the telescopic end of the hydraulic cylinder (210). The driving clamping plate (220) is correspondingly arranged with the detection clamping plate (610). A striking plate (320) is rotatably arranged on the machine shell (20). A striking assembly is further arranged on the machine shell (20). When the driving clamping plate (220) and the detection clamping plate (610) clamp the reaction cup (10) until the detection is completed, the striking plate (320) drives the cylinder (620) to operate through the striking assembly, so as to separate the detection clamping plate (610) from the driving clamping plate (220).

2. The immune cell detector according to claim 1, wherein: A plurality of hollow parts (130) are circumferentially and arrayedly formed on the reaction plate (110). A clamping frame (140) is hinged in the hollow part (130). The clamping frame (140) is set as an arc-shaped clamping hand with a silica gel head at the clamping end.

3. An immune cell detector according to claim 1, characterized in that: A fixed sleeve (230) is installed on the side of the driving clamping plate (220). A elastic sleeve (240) is fixedly installed at the telescopic end of the hydraulic cylinder (210). The elastic sleeve (240) is inserted and engaged with the fixed sleeve (230). The elastic sleeve (240) is set as a sleeve with a spring inside.

4. The immune cell detector according to claim 3, wherein: A synchronous rod (260) is installed on the side of the fixed sleeve (230). A reinforcement base (310) is installed on the side of the machine shell (20). The striking plate (320) is hingedly installed on the reinforcement base (310). A guiding through groove (330) is formed on the striking plate (320). The synchronous rod (260) passes through the guiding through groove (330); The guiding through groove (330) includes a horizontal through groove (331) and an arc through groove (332). The arc through groove (332) is connected to one side of the horizontal through groove (331) adjacent to the reaction cup (10); In the normal state, the synchronous rod (260) is located in the horizontal through groove (331); During detection, the synchronous rod (260) is located in the arc through groove (332).

5. The immune cell detector according to claim 4, wherein: A piston cylinder (710) is installed on the machine shell (20). A return air pipe (720) and an exhaust pipe (730) are connected to the side of the piston cylinder (710). The return air pipe (720) and the exhaust pipe (730) are connected to the cylinder (620). The striking plate (320) is intermittently drivingly connected with the piston cylinder (710).

6. The immune cell detector according to claim 5, characterized in that: The striking assembly comprises a fixed rack (440) fixedly mounted on the housing (20), a striking block (410) slidably mounted in the housing (20), and a piston plate (490) working in the piston cylinder (710), a double-tooth rack (450) being slidably mounted on the side of the fixed rack (440), a connecting block (420) being mounted on the side of the striking block (410), a double-gear (430) being rotatably mounted on the side of the connecting block (420), and the adding The double gear (430) is meshed with the double-tooth rack (450) and the fixed rack (440) at the same time, an output rack (470) is installed on the side of the piston plate (490), a transmission gear (460) is rotatably installed inside the housing (20), and the transmission gear (460) is meshed with the output rack (470) and the double-tooth rack (450) at the same time, and a reset spring (480) for reset is installed between the impact block (410) and the housing (20).

7. The immune cell detector according to claim 4, wherein: A fixing frame (630) is provided in the detection direction of the reaction disk (110), the cylinder (620) is fixedly mounted on the fixing frame (630), a material unloading frame (650) is fixedly mounted in the unloading direction of the fixing frame (630), and the material unloading frame (650) is intermittently docked with the car frame (640); An output wheel shaft (270) is rotatably mounted on the end of the synchronization rod (260), the output wheel shaft (270) is clearance-matched with the horizontal through slot (331), an axis end wheel (280) is fixedly mounted on the end of the output wheel shaft (270), and a reversing rod (290) is mounted on the side of the axis end wheel (280); An L-shaped groove (550) is provided on the housing (20), a reversing plate (510) is slidably mounted in the L-shaped groove (550), the reversing plate (510) is vertically connected to an L-shaped rod (520), the reversing plate (510) and the reversing rod (290) are intermittently engaged, and the L-shaped rod (520) is transmission-connected to the car frame (640).

8. An immune cell detector according to claim 7, characterized in that: A blanking shaft (590) is rotatably mounted on the enclosure (120), the car frame (640) is fixedly mounted on the blanking shaft (590), a driving rod (560) is rotatably mounted on the housing (20), a pulley set (580) is commonly mounted between the blanking shaft (590) and the driving rod (560), a gear roller (570) is coaxially mounted on the blanking shaft (590), a transverse rack (530) is connected to the end of the L-shaped rod (520), and the transverse rack (530) is meshed with the gear roller (570); When the reversing plate (510) rotates and drives the reversing rod (290) to move, the car frame (640) rotates and docks with the unloading frame (650).

9. The immune cell detector according to claim 8, wherein: A compression spring (540) is installed between the swing plate (510) and the wall of the L-shaped groove (550). One end of the car frame (640) adjacent to the detection clamping plate (610) is provided with a corresponding notch (641), and the corresponding end of the detection clamping plate (610) is provided with a docking notch (611).

10. An immune cell detection method, characterized in that: Using the immune cell detector according to any one of claims 1-9, comprising the following specific steps: S1. Sample collection and pretreatment: Collect a biological sample, and pretreat the collected biological sample. First, remove impurities and large cell clumps in the sample through a filtration device. The filtration device uses a filter screen with a pore size of 5-10 microns; then place the filtered sample in a centrifuge and centrifuge it at a rotation speed of 1500-2000 revolutions per minute for 5-10 minutes to separate the supernatant containing immune cells; S2. Immune cell labeling: Add a specific immune cell labeling agent to the supernatant. The specific immune cell labeling agent includes a fluorescent-labeled antibody and a magnetic bead-labeled antibody. Incubate the supernatant with the labeling agent in a constant temperature environment at 37°C for 15-30 minutes to allow the labeling agent to fully bind to the immune cells; S3. Use a magnetic field to process the incubated sample so that the immune cells labeled with magnetic beads are adsorbed on one side of the magnetic field, thereby separating them from other components, and elute the immune cells adsorbed on one side of the magnetic field with an eluent to achieve the enrichment of immune cells; S4. Place the enriched immune cell sample in a reaction cup (10) and place it on a reaction plate (110). Use excitation light of different wavelengths to excite the fluorescent-labeled antibody on the immune cells, detect the fluorescent signals emitted by the immune cells, and transmit the obtained fluorescent signal data to a data processing system. The data processing system uses an algorithm model based on deep learning to process and analyze the data, and generate an immune cell analysis and detection report according to the analysis results. The report content includes information such as the type, quantity, proportion, and various characteristic parameters of the immune cells.

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