Aegiceras corniculatum total saponin extraction method

Abstract

The invention discloses an extraction method of total saponins of Aegiceras corniculatum, and belongs to the technical field of extraction of total saponins. Comprising the following steps: 1, mixing choline chloride as a hydrogen bond acceptor with lactic acid as a hydrogen bond donor, adding water, and heating and stirring in a water bath to obtain a eutectic solvent; and 2, weighing Aegiceras corniculatum leaf coarse powder, adding the Aegiceras corniculatum leaf coarse powder into the eutecticevaporate solvent prepared in the step 1, mixing, putting into an ultrasonic cleaning machine, and filtering after the extracting solution is cooled to room temperature to obtain the Aegiceras corniculatum total saponin extracting solution. According to the extraction method for the total saponins of the aegiceras corniculatum, the extraction rate is greatly improved through optimization of a single-factor experiment, an orthogonal experiment and an artificial neural network program.

Description

Technical Field

[0001] The present application relates to the technical field of saponin extraction, and in particular to a method for extracting total saponins from Tung blossom trees. Background Art

[0002] Aegiceras corniculatum, a plant of the genus Aegiceras in the family Myrsinaceae, is a common mangrove plant, a shrub or small tree that grows on tidal mudflats near the sea. Saponins in Aegiceras corniculatum have antibacterial, antioxidant, antitumor, anticancer, hepatoprotective, and immunity-enhancing activities. However, the prior art lacks a method for extracting total saponins from Aegiceras corniculatum. Summary of the Invention

[0003] The purpose of this application is to provide a method for extracting total saponins from Aegiceras corniculata to improve the extraction rate of total saponins from Aegiceras corniculata.

[0004] The present application provides a method for extracting total saponins from Tung blossom tree, comprising the following steps:

[0005] Step 1: Choline chloride is selected as a hydrogen bond acceptor and mixed with lactic acid as a hydrogen bond donor, water is added, and the mixture is heated in a water bath with stirring to obtain a deep eutectic solvent;

[0006] Step 2: Weigh the coarse powder of Tung blossom tree leaves, add the low eutectic solvent prepared in step 1, mix and place in an ultrasonic cleaner, after the extract is cooled to room temperature, filter to obtain the Tung blossom tree total saponin extract.

[0007] Preferably, in step 1, choline chloride as a hydrogen bond acceptor and lactic acid as a hydrogen bond donor are mixed in a molar ratio of 1:2.2.

[0008] Preferably, in step 1, the amount of water added is 40% of the total amount of choline chloride and lactic acid.

[0009] Preferably, in step 2, the coarse powder of Tung tree leaves is added to the deep eutectic solvent prepared in step 1 at a liquid-to-solid ratio of 70 mL / g.

[0010] Preferably, in step 2, the temperature of ultrasonic extraction is 60°C.

[0011] Preferably, in step 2, the ultrasound-assisted extraction time is 180 minutes.

[0012] Therefore, the present application provides a method for extracting total saponins from Tung blossom tree, which has the following beneficial effects:

[0013] Through single-factor experiments and orthogonal experiments, the effects of multiple factors on the extraction of total saponins from Tung tree were investigated, and the optimal extraction conditions were determined, including the type of low eutectic solvent, water content, molar ratio, liquid-to-solid ratio, ultrasonic temperature and ultrasonic time, thereby improving the extraction efficiency and extraction amount of total saponins from Tung tree. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The results show the effect of deep eutectic solvent type on total saponins in Aegiceras corniculata in a single-factor experiment. DES-1 is a deep eutectic solvent formed by choline chloride and glycerol, DES-2 is a deep eutectic solvent formed by choline chloride and xylitol, DES-3 is a deep eutectic solvent formed by choline chloride and malic acid, DES-4 is a deep eutectic solvent formed by choline chloride and lactic acid, and DES-5 is a deep eutectic solvent formed by choline chloride and urea.

[0015] Figure 2 This is the research result of the effect of water content of deep eutectic solvent on total saponins of Aegiceras corniculata in single factor experiment;

[0016] Figure 3 The results show the effect of the molar ratio of deep eutectic solvent on the total saponins of Aegiceras corniculata in a single factor experiment.

[0017] Figure 4 The results of a single-factor experiment show the effect of extraction solvents on the total saponins of Aegiceras corniculata. DES is a system composed of choline chloride and lactic acid with a molar ratio of 1:2 and a water content of 40%.

[0018] Figure 5 This is the research result of the effect of liquid-solid ratio on total saponins of Tung tree in single factor experiment;

[0019] Figure 6 This is the research result of the effect of ultrasound temperature on total saponins of Aegiceras corniculata in a single factor experiment;

[0020] Figure 7 These are the results of a single-factor experiment on the effect of ultrasound time on total saponins from Tung tree. DETAILED DESCRIPTION

[0021] The technical solution of the present invention is further illustrated by the following examples.

[0022] Example 1

[0023] A method for extracting total saponins from Tung blossom tree, comprising the following steps:

[0024] Step 1: Choline chloride is selected as a hydrogen bond acceptor and lactic acid is selected as a hydrogen bond donor in a molar ratio of 1:2.2. Choline chloride and 40% water of the total amount of lactic acid are added, and then heated in a water bath with stirring until a transparent homogeneous liquid is formed to obtain a deep eutectic solvent.

[0025] Step 2: Weigh the coarse powder of Tung blossom leaves, add the coarse powder of Tung blossom leaves to the low eutectic solvent prepared in step 1 at a liquid-to-solid ratio of 70 mL / g, mix and place in an ultrasonic cleaning machine, the ultrasonic extraction temperature is 60°C, the ultrasonic-assisted extraction time is 180 min, and after the extract is cooled to room temperature, filter to obtain the Tung blossom total saponin extract.

[0026] This application first investigates the effects of DESs type, solvent system water content, DESs molar ratio, conventional solvent, liquid-to-solid ratio, ultrasonic temperature, and ultrasonic time on the extraction of total saponins from Tung tree through single-factor experiments.

[0027] according to Figure 1-Figure 7 The results of the single factor experiment were as follows: taking the extraction rate of total saponins from Tung tree as the index, the four influencing factors of extraction temperature, liquid-to-solid ratio, extraction time, and solvent system molar ratio were selected to design a four-factor three-level orthogonal test scheme. The orthogonal table of L9 (34) was used for the corresponding orthogonal test. It was found that when the ultrasonic temperature was 60℃, the ultrasonic time was 180min, the liquid-to-solid ratio was 80mL / g, and the choline chloride / lactic acid molar ratio was 1:2, the total saponins amount was 100.39mg / g. The results were optimized by artificial neural network. The optimal process conditions for total saponins from Tung tree were ultrasonic temperature 60℃, ultrasonic time 180min, liquid-to-solid ratio 70mL / g, and molar ratio 1:2.2. The results were analyzed with the results of the orthogonal test. The ultrasonic temperature and ultrasonic time were consistent with the molar ratio to a certain extent, while the liquid-to-solid ratio was significantly different from the orthogonal test optimization results. To address this issue, three parallel experiments were conducted on the process conditions obtained by artificial neural network optimization, and the total saponin extraction amounts of Tung tree were 107.86 mg / g, 110.59 mg / g, and 107.07 mg / g, respectively, with an average extraction amount of 108.51 mg / g.

[0028] Therefore, the present application provides a method for extracting total saponins from Tung blossom tree, which is optimized through single-factor experiments, orthogonal experiments and artificial neural network programs, and the extraction rate of the obtained method for extracting total saponins from Tung blossom tree is greatly improved.

[0029] In this specification, references to terms such as "an experimental example," "example," or "specific example" indicate that the specific features, structures, materials, or characteristics described in conjunction with that experimental example or example are included in at least one experimental example or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same experimental example or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more experimental examples or examples.

[0030] Finally, it should be noted that the above experimental examples are only used to illustrate the technical solutions of the present invention rather than to limit them. Although the present invention has been described in detail with reference to the preferred experimental examples, those skilled in the art should understand that they can still modify or replace the technical solutions of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A method for extracting total saponins from Tung blossom tree, characterized in that: The following steps are involved: Step 1: Choline chloride is selected as a hydrogen bond acceptor and mixed with lactic acid as a hydrogen bond donor, water is added, and the mixture is heated in a water bath with stirring to obtain a deep eutectic solvent; Step 2: Weigh the coarse powder of Tung blossom tree leaves, add the low eutectic solvent prepared in step 1, mix and place in an ultrasonic cleaner, after the extract is cooled to room temperature, filter to obtain the Tung blossom tree total saponin extract.

2. The method for extracting total saponins from Tung blossom tree according to claim 1, wherein: In step 1, choline chloride as a hydrogen bond acceptor and lactic acid as a hydrogen bond donor are mixed in a molar ratio of 1:2.

2.

3. The method for extracting total saponins from Tung blossom tree according to claim 1, wherein: In step 1, the amount of water added is 40% of the total amount of choline chloride and lactic acid.

4. The method for extracting total saponins from Tung blossom tree according to claim 1, wherein: In step 2, the crude powder of Tung tree leaves is added to the low eutectic solvent prepared in step 1 at a liquid-to-solid ratio of 70 mL / g.

5. The method for extracting total saponins from Tung blossom tree according to claim 1, wherein: In step 2, the temperature of ultrasonic extraction is 60°C.

6. The method for extracting total saponins from Tung blossom tree according to claim 1, wherein: In step 2, the ultrasonic-assisted extraction time is 180 min.

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