Application of plant source compound acetophenone in prevention and treatment of callosobruchus chinensis

Abstract

The invention discloses application of a plant source compound acetophenone in prevention and treatment of callosobruchus chinensis. The application comprises fumigation and killing of eggs, larvae, pupae and adults of the callosobruchus chinensis. The plant source compound acetophenone is processed into an acceptable preparation for use. The fumigation results of larvae, pupae and adults show that the corrected death rate of the callosobruchus chinensis is gradually increased along with the increase of the acetophenone fumigation concentration and the prolonging of the fumigation time. Wherein under the condition that the fumigation concentration is 10 [mu] L / L, as the fumigation time is prolonged from 24 h to 72 h, the corrected death rate of callosobruchus chinensis adults is increased from 59.55% to 100%; and under the fumigation treatment time of 24 hours, along with the increase of the fumigation concentration from 7 [mu] L / L to 11 [mu] L / L, the corrected death rate of the acetophenone to the callosobruchus chinensis adults is increased from 15.73% to 77.53%.

Description

Technical Field

[0001] The present invention relates to the fields of green agricultural technology and pest control technology, and in particular to application of a plant-derived compound, acetophenone, in controlling green bean weevils. Background Art

[0002] The mung bean weevil is a worldwide stored-grain pest, widely distributed in my country. It primarily feeds on legumes such as mung beans, red beans, and cowpeas, and is characterized by a wide distribution range, short lifespan, strong reproductive capacity, and rapid spread. Adults lay eggs in bean pods stored in warehouses and in the field, carrying them to storage with the harvested beans, causing secondary damage in the warehouses. The mung bean weevil can cause losses of 40% to 70% in the field. In mung bean warehouses, it can consume and damage 90% of the mung beans within 4 to 6 months. The infested beans are typically hollow, turning the seeds into a powdery state, severely reducing the quality of the beans and preventing them from germinating. Furthermore, the weevil's excrement and carcasses, mixed with grain piles, can also cause serious contamination. If left untouched for a long time, the beans can develop mold and produce harmful substances, seriously affecting their edible quality and commercial value.

[0003] Currently, chemical control is the primary method for controlling the green bean weevil during bean storage. However, the overuse of chemical pesticides has led to increasing problems, including toxicity to humans and animals, environmental pollution, pesticide residues, and the development of pest resistance. Some highly toxic and effective chemical pesticides have been banned. To find alternatives to chemical pesticides, developing effective botanical pesticides is crucial. Botanical pesticides are derived from natural plants, are environmentally safe, highly specific, have low toxicity to non-target organisms, are easily degradable, leave low residues, and are less likely to induce pest resistance.

[0004] Therefore, the existing technology has defects and needs to be improved. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide an application of a plant-derived compound acetophenone in preventing and controlling green bean weevils in response to the deficiencies in the prior art.

[0006] The technical solutions of the present invention are as follows: Application of the plant-derived compound acetophenone in controlling green bean weevils.

[0007] The application includes fumigation and killing of eggs, larvae, pupae and adults of mung bean weevil.

[0008] In the application, the plant-derived compound acetophenone is processed into an acceptable preparation and then used.

[0009] The application of the invention is to investigate the LC of acetophenone fumigated mung bean weevil eggs under the conditions of 24 h, 48 h and 72 h.50 The value is less than 0.01 μL / L, and the LC for larvae 50 The values ​​were 2.13 μL / L, 1.73 μL / L and 1.66 μL / L, respectively, and the LC values ​​for pupae were 50 The values ​​were 2.66 μL / L, 2.30 μL / L and 2.02 μL / L, respectively, and the LC values ​​for adults were 50 The values ​​were 1.31 μL / L, 1.18 μL / L, and less than 1.20 μL / L, respectively.

[0010] Acetophenone was the most effective fumigant against eggs of the green bean weevil, achieving a corrected mortality rate of 100%. Fumigation results for larvae, pupae, and adults showed that the corrected mortality rate of the weevil gradually increased with increasing acetophenone fumigation concentration and prolonged fumigation time. Specifically, at a fumigation concentration of 10 μL / L, the corrected mortality rate of weevil adults increased from 59.55% to 100% as the fumigation time increased from 24 to 72 hours. At a 24-hour fumigation time, the corrected mortality rate of acetophenone against weevil adults increased from 15.73% to 77.53% as the fumigation concentration increased from 7 to 11 μL / L. DETAILED DESCRIPTION

[0011] The present invention is described in detail below with reference to specific embodiments.

[0012] 1. Test insects The mung bean weevil is a long-term breeding population of the Key Laboratory of Insects of Shanxi Agricultural University; the mung bean used in the test is Jinlu No. 1.

[0013] Rearing Method: Place clean mung beans in a 9 cm diameter Petri dish, inoculate male and female adults of the green bean weevil, and culture in an incubator. After 24 hours of egg laying, remove the adults. Select a portion of mung beans with only a single egg attached (if multiple eggs are attached, remove the excess eggs) for the egg fumigation test. Continue culture in the incubator with another portion of mung beans with a single egg. Remove a portion at 12 days for the larval fumigation test, and another portion at 18 days for the pupal fumigation test. For the adult fumigation test, select green bean weevil species that have emerged within 24 hours.

[0014] Rearing conditions: temperature 28 ± 1 °C, relative humidity 75 ± 5%, and photoperiod 16 L:8 D.

[0015] 2. Test reagents Acetophenone (CAS No.: 98-86-2; purity: ≥99%) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0016] 3. Test methods A closed fumigation method was used to test the fumigation effect of the green bean weevil (Eriophorus spp.). 500 mL reagent bottles were used as fumigation containers, with 30 infested green beans at different developmental stages or 30 adult green beetles in each bottle. Acetophenone of varying concentrations was pipetted onto filter paper strips (4 cm × 1 cm) using a micropipette. The strips were then threaded with cotton thread and hung approximately 5 cm from the bottom of the bottle, ensuring they did not come into contact with the inner wall. To prevent direct contact with the acetophenone and potentially causing contact damage to the adult beetles, the filter paper strips were placed in 1.5 mL centrifuge tubes, sealed with gauze and wire, and placed at the bottom of the reagent bottle. After addition of the agent, the bottles were immediately capped and placed in an incubator at 28 ± 1°C and 75 ± 5% relative humidity in the dark. Three fumigation durations of 24 h, 48 h, and 72 h were set. Experiments without acetophenone served as a blank control, and each oil-treated group and the blank control were replicated three times.

[0017] After fumigation, observe and record the number of adult green bean weevils that have died. Egg, larval, and pupal stages must be placed in 15 mL centrifuge tubes with punctured spiracles for observation. After development and eclosion, observe the number of adults in both the control and treatment stages that have emerged. Calculate the adjusted mortality rate using the following formula:

[0018] 4. Results and Analysis The fumigation toxicity of acetophenone against Weevil (Coleoptera: Amygdalus) was determined, and the corrected mortality rates for Weevil (Coleoptera: Amygdalus) at different treatment times and concentrations were obtained. The results are shown in Table 1. As shown in Table 1, acetophenone was most effective against Weevil eggs, achieving a corrected mortality rate of 100%. The fumigation results for larvae, pupae, and adults showed that the corrected mortality rates of Weevil increased with increasing acetophenone fumigation concentration and duration. Specifically, at a fumigation concentration of 10 μL / L, the corrected mortality rate of Weevil adults increased from 59.55% to 100% as the fumigation duration increased from 24 to 72 hours. At a fumigation duration of 24 hours, the corrected mortality rate of acetophenone against Weevil adults increased from 15.73% to 77.53% as the fumigation concentration increased from 7 to 11 μL / L.

[0019] According to the fumigation results in Table 1, the logarithmic value of the fumigation concentration of acetophenone on green bean weevil and the corrected mortality value were fitted to obtain the regression equation of the fumigation toxicity of acetophenone on green bean weevil and the median lethal concentration (LC 50 ) etc. The results are shown in 2. As can be seen from the table, the LC values ​​of mung bean weevil eggs fumigated with acetophenone were 24 h, 48 h and 72 h respectively. 50 The value is less than 0.01 μL / L, and the LC for larvae 50The values ​​were 2.13 μL / L, 1.73 μL / L and 1.66 μL / L, respectively, and the LC values ​​for pupae were 50 The values ​​were 2.66 μL / L, 2.30 μL / L and 2.02 μL / L, respectively, and the LC values ​​for adults were 50 The values ​​were 1.31 μL / L, 1.18 μL / L and less than 1.20 μL / L, respectively. This indicates that the fumigation effect of acetophenone on different developmental stages of green bean weevil is eggs>adults>larvae>pupa.

[0020] Table 1 Fumigation effects of acetophenone on different developmental stages of green bean weevil

[0021] Note: The data in the table are "mean ± standard error". Different lowercase letters after the data in the same column indicate significant differences among treatments (one-way analysis of variance, Tukey's test, P<0.05).

[0022] Table 2 Regression analysis of the fumigation toxicity of acetophenone to different developmental stages of Cercidiphyllum spp.

[0023] It should be understood that those skilled in the art can make improvements or changes based on the above description, and all such improvements and changes should fall within the scope of protection of the appended claims of the present invention.

Claims

1. Application of the plant-derived compound acetophenone in controlling mung bean weevils.

2. The use according to claim 1, comprising fumigating and killing eggs, larvae, pupae and adults of mung bean weevil.

3. The use according to claim 1, wherein the plant-derived compound acetophenone is processed into an acceptable preparation before use.

4. The use according to claim 1, wherein the LC of acetophenone fumigated mung bean weevil eggs is 24 h, 48 h and 72 h. 50 The value is less than 0.01 μL / L, and the LC for larvae 50 The values ​​were 2.13 μL / L, 1.73 μL / L and 1.66 μL / L, respectively, and the LC values ​​for pupae were 50 The values ​​were 2.66 μL / L, 2.30 μL / L and 2.02 μL / L, respectively, and the LC values ​​for adults were 50 The values ​​were 1.31 μL / L, 1.18 μL / L, and less than 1.20 μL / L, respectively.

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