Thermal cover assembly of gene amplification instrument
By designing a combination of multiple independent seals and pressure rod return springs on the PCR instrument, the problem of difficult sealing of the PCR instrument's hot cover is solved, effective sealing of the reagents in the sample tube is achieved, the accuracy of the test results is improved, and maintenance costs are reduced.
Patent Information
- Application Number
- CN202410408413.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-07
- Publication Date
- 2025-10-14
AI Technical Summary
The flat design of the thermal cover of existing PCR instruments makes it difficult to effectively seal the sample tube, causing the reagent to evaporate or escape, affecting the accuracy of biochemical test results.
Multiple independent seals are matched with the sample tubes. The sample tubes are sealed by the elastic deformation and self-adaptation of the seals. Combined with the design of the pressure rod and the return spring, the sample tubes are individually sealed.
It effectively prevents the evaporation or escape of reagents in the sample tube, improves the accuracy of biochemical test results, and maintains a good sealing effect even if there are mechanical errors or assembly errors, with low maintenance costs.
Smart Images
Figure CN120775664A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of gene detection, and in particular to a thermal cover assembly of a gene amplification instrument. Background Art
[0002] PCR (Polymerase Chain Reaction) uses a segment of DNA as a template. With the participation of DNA polymerase and nucleotide substrate, the segment of DNA is amplified to a sufficient amount for structural and functional analysis.
[0003] During the PCR amplification reaction, there will be a thermal reaction with a high temperature, a long reaction time or a rapid temperature change. Due to the rapid temperature change and the large temperature difference between the PCR gene amplification process and the ambient temperature, the reagents will inevitably evaporate seriously, thereby generating aerosols. If the aerosols overflow, it will cause pollution problems, affect the normal progress of the reaction, and affect the accuracy of the biochemical test results. Therefore, the PCR instruments in the prior art are all provided with a hot cover (a metal plate that is basically flat). Since the hot cover is heated and its temperature is higher than the temperature of the reagents, the principle of cold air downward movement can be used to not only avoid the overflow of aerosols, but also prevent the reagents from condensing on the upper hot cover, thereby ensuring the accuracy of the biochemical test results.
[0004] However, since the thermal cover itself is a flat product, it is difficult to seal the sample tube simply by the flat contact between the thermal cover and the sample tube, and the problem of reagent evaporation or leakage still exists. Summary of the Invention
[0005] In order to solve the above technical problems, the present invention provides a gene amplification instrument thermal cover assembly and a gene amplification instrument, which seal the sample tube through multiple independent sealing parts and have good sealing effect.
[0006] The present invention adopts the following technical solutions:
[0007] A thermal cover assembly for a gene amplification instrument comprises: a thermal cover support; a thermal cover mounted on the thermal cover support; and a plurality of sealing members independently disposed on the thermal cover for sealing against sample tubes of a multi-well plate placed on a body of the gene amplification instrument. The plurality of independent sealing members seal each sample tube individually, resulting in a good sealing effect and the ability to effectively prevent evaporation or escape of reagents in the sample tubes.
[0008] Preferably, the sealing member is made of rubber or silicone with a Shore A hardness of 10-100. When sealed on the sample tube, it can produce a certain deformation and fit on the opening of the sample tube to better seal the sample tube.
[0009] Preferably, a circle of annular protrusions is formed at the open end of the sample tube, and an annular groove is provided inside the seal at a position corresponding to the annular protrusion, so that the seal parts on both sides of the annular groove are squeezed toward the recessed part, so that the seal can fully wrap the open end of the sample tube, thereby improving the sealing effect.
[0010] Preferably, the thermal cover assembly further comprises a plurality of pressure rods mounted on the thermal cover, and the end of each pressure rod facing the sample tube is provided with the sealing member; the sealing member and the pressure rods are movable relative to the thermal cover along the axis of the pressure rods, and the product has better adaptability: even if the surface of the multi-well plate or the thermal cover is uneven, or there are mechanical errors or assembly errors, each sealing member can adapt to each sample tube to form a reliable seal.
[0011] Preferably, the seal and the pressure rod can be displaced relative to the thermal cover in a first direction after being subjected to the force of the sample tube, and can be displaced relative to the thermal cover in a second direction after being out of contact with the sample tube; the first direction and the second direction are opposite.
[0012] Preferably, the thermal cover assembly further comprises: a positioning plate disposed on the thermal cover; a plurality of pressure rods, each of which has the sealing member at its end facing the sample tube, and which penetrate the thermal cover and are movable along their axial direction relative to the thermal cover and the positioning plate; and a plurality of return springs sleeved on the pressure rods, with one end abutting against the positioning plate and the other end abutting against the pressure rods, such that the return springs can press the sealing member tightly against the sample tube to achieve sealing.
[0013] Preferably, the end of the pressure rod away from the seal passes through the positioning plate and is provided with a limiting ring to prevent the pressure rod from falling off the positioning plate. The pressure rod is prevented from falling off the positioning plate by the limiting ring, thereby limiting the pressure rod.
[0014] Preferably, the pressure rod includes a pressure rod body, a limiting portion formed on the pressure rod body for preventing the seal from falling off, and a convex portion formed at the end of the pressure rod body; the seal is covered on the limiting portion and the convex portion.
[0015] Preferably, an annular groove is provided on the sealing member at a position away from one end of the sample tube and corresponding to the annular protrusion of the sample tube. The annular groove is located on the outer circle of the protrusion, and its open end abuts against the limiting portion.
[0016] Preferably, the end of the protrusion is exposed outside the sealing member to facilitate heat conduction and improve thermal efficiency.
[0017] Preferably, a plurality of guide holes are formed on the heat cover bracket, and a plurality of guide posts coaxially passing through the guide holes are formed on the heat cover. A compression spring is provided on the outer sleeve of the guide post, and the two ends of the compression spring respectively abut against the heat cover bracket and the heat cover. In this way, the heat cover assembly has a larger range of compensation capabilities.
[0018] Preferably, the thermal cover assembly further comprises: a thermal cover housing; a handle rotatably mounted on the thermal cover housing via a rotating shaft, the handle being provided with at least two arc-shaped guide grooves; a pin mounted on the thermal cover bracket, the ends of the pin being respectively embedded in the two arc-shaped guide grooves; and the thermal cover bracket, the thermal cover and the sealing member being able to move relative to the thermal cover housing between a first position and a second position by rotating the handle.
[0019] Preferably, when in the second position, the sealing member seals each sample tube of the multi-well plate one by one; when in the first position, the sealing member is released from sealing contact with each sample tube of the multi-well plate.
[0020] Preferably, the handle is further provided with a locking hook for cooperating with a locking post on the thermal cover shell to achieve locking and positioning of the thermal cover assembly of the gene thermal amplification instrument and the body of the gene thermal amplification instrument.
[0021] Preferably, in the second position, the lock hook and the lock cylinder cooperate to lock; in the first position, the lock hook and the lock cylinder are separated.
[0022] Compared with the prior art, the present invention has the following advantages:
[0023] 1. The present invention provides a thermal cover assembly for a gene thermal amplifier, comprising a thermal cover bracket, a thermal cover, and several sealing components. Multiple independent sealing components are used to individually seal each sample tube. Compared to conventional solutions that use flat thermal covers for sealing, this assembly offers superior sealing performance, effectively preventing evaporation or escape of reagents within the sample tubes. Furthermore, even if a seal fails due to aging or other factors, resulting in a poor sealing effect, it can be simply replaced, resulting in low overall maintenance costs.
[0024] 2. Since each pressure rod and the seal installed on the pressure rod can move relative to the thermal cover along the axis of the pressure rod, the product has better adaptability: even if the surface of the multi-well plate or the thermal cover is uneven, or there are mechanical errors or assembly errors, each seal can adapt to each sample tube to form a reliable seal.
[0025] 3. An annular protrusion is formed at the open end of the sample tube, and an annular groove is formed on the seal at a position away from one end of the sample tube and corresponding to the annular protrusion. After the seal is squeezed by the annular protrusion of the sample tube, the parts on both sides of the annular groove will undergo a certain outward deformation toward the outer circle. Combined with the squeezing effect of the annular protrusion of the sample tube from the bottom, the deformed seal can squeeze the annular protrusion of the sample tube from the side, so that the seal can fully wrap the open end of the sample tube, thereby improving the sealing effect.
[0026] 4. The present invention only needs to turn the handle downward to achieve locking. At the same time, the hot cover bracket, hot cover and sealing member move downward, and each sample tube is individually sealed by multiple independent sealing members, with good sealing effect. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] Figure 1 Schematic diagram of the hot cover assembly in use.
[0028] Figure 2 A cross-sectional view of the thermal cover assembly.
[0029] Figure 3 This is a schematic diagram of the structure when the seal is out of contact with the sample tube.
[0030] Figure 4 This is a schematic diagram of the structure when the seal is sealing the sample tube.
[0031] Figure 5 for Figure 4 A partial enlarged view of .
[0032] Figure 6 A partial cross-sectional view of the thermal cover assembly.
[0033] Figure 7 for Figure 6 A partial enlarged view of part C.
[0034] Figure 8 Schematic diagram of the open state of the thermal cover assembly.
[0035] Figure 9 Schematic diagram of the assembly of the hot cover and positioning plate.
[0036] In the figure, the heat cover bracket 1, the guide hole 1-1, the heat cover 2, the guide column 2-1, the seal 3, the annular groove 3-1, the pressure rod 4, the pressure rod body 4-1, the limit portion 4-2, the protrusion 4-3, the positioning plate 5, the return spring 6, the limit ring 7, the compression spring 8, the heat cover shell 9, the handle 10, the arc guide groove 10-1, the lock hook 10-2, the pin 11, the lock column 12, and the heat cover lower shell 13. DETAILED DESCRIPTION
[0037] In order to facilitate understanding of the technical solution of the present invention, the following is a detailed description with reference to the accompanying drawings and specific embodiments.
[0038] like Figures 1-4 As shown, a gene thermal cycler hot cover assembly includes:
[0039] Hot cover support 1;
[0040] A thermal cover 2 is mounted on the thermal cover support 1;
[0041] Several seals 3 are independently arranged on the thermal cover 2 and are used to achieve sealing with each sample tube of the porous plate placed on the gene amplification instrument body. In this embodiment, the seal 3 is made of rubber or silicone with a Shore A hardness of 10-100. When the seal 3 is pressed against the sample tube, it can produce a certain deformation, fit on the opening of the sample tube, and better seal the sample tube. At the same time, its Shore A hardness is 10-100, which is not too soft (if it is too soft, the interaction force between the seal and the sample tube is also small, resulting in poor sealing effect), nor too hard (if it is too hard, the seal deformation is small, resulting in poor sealing effect). Since the seal 3 itself has a certain elastic deformation ability, even if the surface of the porous plate or the thermal cover is uneven, each seal 3 can adapt to each sample tube to form a reliable seal.
[0042] In some practical applications, the thermal cover assembly further includes a plurality of pressure rods 4 mounted on the thermal cover 2, each pressure rod 4 having a seal 3 disposed on its end facing the sample tube; the seals 3 and pressure rods 4 are capable of moving axially relative to the thermal cover 2 along the pressure rods 4. Thus, because each pressure rod 4 and the seals 3 mounted thereon are capable of moving axially relative to the thermal cover 2 along the pressure rods 4, the product has greater adaptability: even if the surface of the multi-well plate or the thermal cover is uneven, or there are mechanical or assembly errors, each seal 3 can adapt to each sample tube and form a reliable seal. In practical applications, the seals 3 and pressure rods 4 can move axially relative to the thermal cover 2 along the pressure rods 4. This can be achieved by a motor-driven gear rack structure or by a spring (the spring is compressed and reset to achieve reciprocating motion). In other practical applications, the seals 3 and pressure rods 4 can be displaced relative to the thermal cover 2 in a first direction upon application of a force applied by the sample tube, and can be displaced in a second direction relative to the thermal cover 2 after being released from contact with the sample tube; the first and second directions are opposite. Specifically, the second direction is the direction from the sealing member 3 toward the sample tube.
[0043] like Figure 6 、 Figure 7 As shown, in some other practical applications, the thermal cover assembly further includes:
[0044] A positioning plate 5 is provided on the heat cover 2;
[0045] A plurality of pressure rods 4, each of which is provided with the sealing member 3 at its end facing the sample tube, passes through the thermal cover 2, and is movable along its axial direction relative to the thermal cover 2 and the positioning plate 5;
[0046] A plurality of return springs 6 are sleeved on the outside of the pressure rod 4 in a one-to-one corresponding manner, with one end abutting against the positioning plate 5 and the other end abutting against the pressure rod 4.
[0047] The thermal cover 2 has several through-holes that match the pressure rods 4. The pressure rods 4 can slideably fit within the thermal cover 2 and move axially within the through-holes relative to the thermal cover 2 and the positioning plate 5. When the thermal cover assembly is closed, the seal 3 is deformed by the force applied by the sample tube, thereby achieving sealing contact with the sample tube. Furthermore, the return spring 6 is compressed (meaning that the pressure rods 4 move upward relative to the thermal cover 2). Under the action of the return spring 6, the seal 3 is pressed tightly against the sample tube, achieving a better seal. For ease of understanding, the following examples are provided for illustration, but are not to be construed as limiting the present invention. For example, during the closing of the hot lid assembly, the seal 3 and pressure rod 4 descend with the hot lid assembly. After contacting the sample tube, the seal 3 continues to descend, subject to the force of the sample tube and causing deformation. As the hot lid assembly continues to close, the seal 3 and pressure rod 4 continue to descend with the hot lid assembly, further deforming until no further deformation occurs. As the hot lid assembly continues to close, the seal 3 and pressure rod 4 no longer descend but instead ascend relative to the hot lid 2. At this point, the return spring 6 is compressed, maintaining the relative positions of the seal 3, pressure rod 4, and the sample tube unchanged. Simultaneously, the return spring 6 presses the seal 3 against the sample tube, achieving a better seal. During the opening of the hot lid assembly, the opposite occurs: the seal 3 and pressure rod 4 can descend relative to the hot lid 2 under the action of the return spring 6 after disengaging from the sample tube, or they can descend relative to the hot lid 2 under the action of the return spring 6 while in contact with the sample tube.
[0048] like Figure 9 As shown, the positioning plate 5 is provided with a plurality of through holes that cooperate with the pressure rod 4. The end of the pressure rod 4 away from the seal 3 passes through the through hole of the positioning plate 5, and is provided with a limiting ring 7 to prevent the pressure rod 4 from falling off the positioning plate 5. The pressure rod 4 slides into the through hole of the positioning plate 5, and the pressure rod 4 is prevented from falling off from the positioning plate 5 by the limiting ring 7, thereby limiting the pressure rod 4.
[0049] like Figure 3 As shown, in some practical applications, the open end of the sample tube is formed with a circle of annular protrusions, and the seal 3 is provided with an annular groove 3-1 at a position corresponding to the annular protrusion, that is, the upper end in the direction shown in the figure has an annular groove 3-1. Figure 4As shown, when the seal 3 is pressed against the sample tube, the lower surface of the seal 3 abuts against the annular protrusion of the sample tube. Under the pressing action of the annular protrusion, the lower surface of the seal 3 is forced to be concave upward to form a concave portion that accommodates the annular protrusion, and the elastic deformation of the seal 3 is used to achieve sealing contact with the sample tube; at the same time, due to the presence of the annular groove 3-1, after the seal 3 is squeezed by the annular protrusion of the sample tube, the parts located on both sides of the annular groove 3-1 will undergo a certain outward deformation toward the outer circle, and cooperate with the squeezing action of the annular protrusion of the sample tube from the bottom, so that the seal 3 can squeeze the annular protrusion of the sample tube from the side after deformation (as shown in FIG. Figure 5 The sealing member 3 fully wraps the open end of the sample tube, thereby improving the sealing effect.
[0050] like Figure 3 、 Figure 4 As shown, the plunger 4 includes a plunger body 4-1, a stopper 4-2 formed on the plunger body 4-1 for preventing the seal 3 from falling off, and a protrusion 4-3 formed at the end of the plunger body 4-1. The seal 3 covers the stopper 4-2 and the protrusion 4-3 to prevent the seal 3 from falling off the plunger 4. In actual applications, the reagent in the sample tube needs to be heated to 90-95 degrees Celsius. To prevent the reagent from evaporating or escaping, the temperature at the sample tube opening (at the plunger and the seal) needs to be maintained at 105-115 degrees Celsius. Therefore, to reduce energy consumption and improve thermal efficiency, the preferred solution of this embodiment is that the seal 3 is through-hole in the middle, the plunger 4 is made of metal, and the end of its protrusion 4-3 is exposed from the middle of the seal 3 to facilitate heat conduction and reduce the temperature requirement of the heating plate on the thermal cover 2. (If the seal completely covers the end of the plunger, due to the poor thermal conductivity of the seal, a higher temperature is required to ensure that the temperature of the sample tube opening is maintained at the temperature required for PCR amplification.) In this structure, in order to prevent the reagent from evaporating or escaping from the gap between the sealing member 3 and the pressure rod 4, in this embodiment, the annular groove 3-1 of the sealing member 3 is located on the outer circle of the protrusion 4-3, and its open end abuts against the limiting portion 4-2. Figure 4As shown, when the seal 3 is pressed against the sample tube, the lower surface of the seal 3 abuts against the annular protrusion of the sample tube. Under the pressure of the annular protrusion, the lower surface of the seal 3 is forced upward to form a recessed portion that accommodates the annular protrusion. The seal 3 utilizes the elastic deformation to achieve sealing contact with the sample tube. At the same time, due to the presence of the annular groove 3-1, after being squeezed by the annular protrusion of the sample tube, the portions of the seal 3 located on both sides of the annular groove 3-1 will undergo a certain degree of outward deformation toward the outer circle. The portion of the seal 3 located inside the annular groove 3-1 will deform toward the sidewall of the protrusion 4-3 and compress the sidewall of the protrusion 4-3, thereby improving the sealing effect between the seal 3 and the pressure rod 4. Of course, within the scope of choice of those skilled in the art, the protrusion 4-3 can also be completely enclosed by the seal 3 and not exposed.
[0051] The heat cover bracket 1 is formed with a plurality of guide holes 1-1, and the heat cover 2 is formed with a plurality of guide posts 2-1 coaxially passing through the guide holes 1-1. The guide posts 2-1 are provided with compression springs 8 on their outer sleeves, and the two ends of the compression springs are respectively against the heat cover bracket 1 and the heat cover 2, so that the heat cover assembly has a larger range of compensation capabilities.
[0052] like Figure 6-8 As shown, in some practical applications, the thermal cover assembly further includes:
[0053] Thermal cover housing 9; In some practical applications, the thermal cover housing 9 is made of plastic material;
[0054] The handle 10 is rotatably mounted on the heat cover shell 9 via a rotating shaft and is provided with at least two arc-shaped guide grooves 10-1;
[0055] The pin 11 is mounted on the heat cover bracket 1, and its two ends are respectively embedded in two arc-shaped guide grooves 10-1; by rotating the handle 10, the heat cover bracket 1, the heat cover 2 and the seal 3 can be moved between a first position and a second position relative to the heat cover housing 9; in the second position, the seal 3 seals each sample tube of the multi-well plate one by one; in the first position, the seal 3 is released from the sealing contact with each sample tube of the multi-well plate. Since the heat cover assembly is mounted on the gene amplification instrument body by a hinge in the prior art, when the heat cover assembly is closed, the heat cover assembly and the heat cover are pressed against the sample tube by rotation, which causes the sample tube near the hinge side to contact the heat cover first and be subjected to greater pressure. Over time, the seal near the hinge side will be more likely to fail, reducing the service life of the product; after adopting the solution of this embodiment, the movement of the heat cover 2 from the first position to the second position is a linear motion, which can ensure that the pressure on the seal 3 on the entire surface is basically the same, extending the service life of the product and ensuring the sealing effect.
[0056] In some practical applications, a trapezoidal pressure block is provided on the pin 11, and the trapezoidal pressure block is located above the thermal cover support 1. The arcuate guide groove 10-1 has a first end (point A) and a second end (point B). When the thermal cover assembly is in the open state, the pin 11 is located at the second end B of the arcuate guide groove 10-1, and the thermal cover support 1, the thermal cover 2 and the sealing member 3 are in the first position. When in use, the handle 10 is turned downward, and the pin 11 moves relative to the arcuate guide groove 10-1 (from point B of the arcuate guide groove 10-1 to point A), and under the action of the wall surface of the arcuate guide groove 10-1, it drives the thermal cover support 1, the thermal cover 2 and the sealing member 3 to move downward to seal the sample tube.
[0057] In other practical applications, the handle 10 is further provided with a locking hook 10-2 for cooperating with a locking pin 12 located on the thermal cover housing 9 of the gene thermal cycler to lock and position the thermal cover assembly of the gene thermal cycler with the gene thermal cycler body. A through hole is provided on the lower surface of the thermal cover lower shell 13 at a position corresponding to the locking pin 12. When the thermal cover lower shell 13 is closed on the gene thermal cycler body, the locking pin 12 is located within the through hole. At this time, by rotating the handle 10 downward, the pin 11 moves relative to the arcuate guide groove 10-1 (from point B to point A of the arcuate guide groove 10-1), thereby locking the locking hook 10-2 with the locking pin 12, and the thermal cover bracket 1, thermal cover 2, and seal 3 are in the second position. By rotating the handle 10 in the opposite direction, the locking hook 10-2 disengages the locking pin 12, forcing the pin 11 to move from point A to point B of the arcuate guide groove 10-1, and driving the thermal cover bracket 1, thermal cover 2, and seal 3 to move upward, so that the thermal cover bracket 1, thermal cover 2, and seal 3 return to the first position.
[0058] In addition, the multi-well plate placed in the gene amplification instrument body can be a 96-well plate, or a 48-well, 32-well or other number of multi-well plates, just by replacing the hot cover assembly with different numbers of wells.
[0059] The above are only preferred embodiments of the present invention. The scope of protection of the present invention shall be based on the scope defined by the claims. Several improvements and modifications made by those skilled in the art without departing from the spirit and scope of the present invention should also be regarded as the scope of protection of the present invention.
Claims
1. A thermal cover assembly for a gene thermal amplifier, characterized in that: include: Hot cover support (1); A heat cover (2) is mounted on the heat cover support (1); A plurality of sealing members (3) are independently arranged on the heat cover (2) and are used to achieve sealing in a one-to-one correspondence with each sample tube of the multi-well plate placed on the gene amplification instrument body.
2. The thermal cover assembly of the gene thermal cycler according to claim 1, characterized in that: The sealing member (3) is made of rubber or silicone material, and its Shore A hardness is 10-100.
3. The thermal cover assembly of the gene thermal cycler according to claim 1, characterized in that: The open end of the sample tube is formed with a circle of annular protrusions, and an annular groove (3-1) is formed inside the sealing member (3) at a position corresponding to the annular protrusion.
4. The thermal cover assembly of the gene thermal cycler according to claim 1, characterized in that: The thermal cover assembly further comprises a plurality of pressure rods (4) mounted on the thermal cover (2), and the end of each pressure rod (4) facing the sample tube is provided with the sealing member (3); the sealing member (3) and the pressure rod (4) are capable of moving relative to the thermal cover (2) along the axial direction of the pressure rod (4).
5. The thermal cover assembly of the gene thermal amplifier according to claim 4, characterized in that: The sealing member (3) and the pressure rod (4) are capable of displacement relative to the thermal cover (2) along a first direction after being subjected to a force from the sample tube, and are capable of displacement relative to the thermal cover (2) along a second direction after being released from contact with the sample tube; the first direction and the second direction are opposite.
6. The thermal cover assembly of the gene thermal cycler according to claim 1, characterized in that: The thermal cover assembly further comprises: A positioning plate (5) is arranged on the heat cover (2); A plurality of pressure rods (4), each of which is provided with the sealing member (3) at its end facing the sample tube, passes through the thermal cover (2), and is capable of moving along its axial direction relative to the thermal cover (2) and the positioning plate (5); A plurality of return springs (6) are sleeved outside the pressure rod (4), with one end abutting against the positioning plate (5) and the other end abutting against the pressure rod (4).
7. The thermal cover assembly of the gene thermal cycler according to claim 6, characterized in that: One end of the pressure rod (4) away from the sealing member (3) passes through the positioning plate (5), and is provided with a limiting ring (7) for preventing the pressure rod (4) from falling off the positioning plate (5).
8. The thermal cover assembly of the gene thermal cycler according to claim 4, characterized in that: The pressure rod (4) comprises a pressure rod body (4-1), a limiting portion (4-2) formed on the pressure rod body (4-1) for preventing the sealing member (3) from falling off, and a convex portion (4-3) formed at the end of the pressure rod body (4-1); the sealing member (3) is covered on the limiting portion (4-2) and the convex portion (4-3).
9. The thermal cover assembly of the gene thermal amplifier according to claim 8, characterized in that: An annular groove (3-1) is provided inside the sealing member (3) at a position corresponding to the annular protrusion of the sample tube. The annular groove is located on the outer circle of the protrusion (4-3), and its open end abuts against the limiting portion (4-2).
10. The thermal cover assembly of the gene thermal cycler according to claim 9, characterized in that: The end of the protrusion (4-3) is exposed outside the sealing member (3).
11. The thermal cover assembly of the gene thermal cycler according to claim 1, characterized in that: A plurality of guide holes (1-1) are formed on the heat cover bracket (1), and a plurality of guide posts (2-1) coaxially penetrating the guide holes (1-1) are formed on the heat cover (2). A compression spring (8) is provided on the outer sleeve of the guide post (2-1), and two ends of the compression spring respectively abut against the heat cover bracket (1) and the heat cover (2).
12. The thermal cover assembly of the gene thermal cycler according to claim 1, characterized in that: The thermal cover assembly further comprises: Thermal cover housing (9); A handle (10) is rotatably mounted on the heat cover shell (9) via a rotating shaft and is provided with at least two arc-shaped guide grooves (10-1); A pin shaft (11) is mounted on the heat cover bracket (1), with both ends of the pin shaft respectively embedded in two arc-shaped guide grooves (10-1); by rotating the handle (10), the heat cover bracket (1), the heat cover (2) and the sealing member (3) can be moved between a first position and a second position relative to the heat cover housing (9).
13. The thermal cover assembly of the gene thermal cycler according to claim 12, characterized in that: When in the second position, the sealing member (3) seals each sample tube of the multi-well plate one by one; when in the first position, the sealing member (3) breaks away from the sealing contact with each sample tube of the multi-well plate.
14. The thermal cover assembly of the gene thermal cycler according to claim 12, characterized in that: The handle (10) is also provided with a locking hook (10-2) for cooperating with a locking column (12) located on the thermal cover shell (9) to achieve locking and positioning of the thermal cover assembly of the gene amplification instrument and the body of the gene amplification instrument.
15. The thermal cover assembly of the gene thermal cycler according to claim 14, characterized in that: When in the second position, the locking hook (10-2) and the locking column (12) cooperate to lock; when in the first position, the locking hook (10-2) and the locking column (12) are separated.