A method for preparing chufa distillate oil

Abstract

The application discloses a preparation method of chufa diester oil and belongs to the technical field of diester oil preparation. The preparation method of chufa diester oil comprises the following steps: S1. After chufa is dried and crushed, PBS buffer solution and pectinase composite particles are added, and then enzymolysis is carried out under an alternating magnetic field to obtain an enzymolysis liquid; S2. The enzymolysis liquid is subjected to magnetic separation to recover the pectinase composite particles, and then the oil and fat in the supernatant is collected after centrifugation to obtain chufa oil and fat; and S3. The chufa oil and fat, glycerol and lipase composite particles are subjected to light-magnetic fixed catalysis and magnetic separation to recover the lipase composite particles to obtain chufa diester oil. The application solves the problems of low extraction rate, poor selectivity and serious pollution by means of magnetic field-light field synergistic regulation, and provides an efficient, precise and sustainable solution for functional diester oil manufacturing. The oil and fat extraction rate is improved to 90%, the 1,3-diester selectivity is 88%, and the total diester yield is greater than 90%.

Description

Technical Field

[0001] This invention belongs to the field of diester oil preparation technology, specifically relating to a method for preparing tiger nut diester oil. Background Technology

[0002] Tiger nuts (Cyperus esculentus) are a high-value oilseed crop with an oil content of 20-30%, rich in oleic acid (60-70%) and linoleic acid (15-20%), and possess natural antioxidants (such as tocopherols and phytosterols). These two monounsaturated and polyunsaturated fatty acids have been proven to be beneficial to cardiovascular health. Diester oil prepared from tiger nuts can retain this property, or its fatty acid composition can be directionally adjusted through enzymatic catalysis to further enhance its potential for regulating blood lipids. Tiger nut oil is rich in natural antioxidants such as tocopherols (vitamin E) and phytosterols. Under low-temperature enzymatic processing, these components can be retained, improving the oxidative stability of the diester oil, extending its shelf life, and potentially enhancing its anti-inflammatory and anti-atherosclerotic effects.

[0003] Traditional methods for preparing tiger nuts oil have the following limitations:

[0004] Cold pressing: Low temperature preserves active ingredients, but the oil yield is only 15-18%, and the residue has a high oil content.

[0005] Solvent leaching method: hexane extraction efficiency is high (>95%), but high-temperature desolventization (>120℃) damages heat-sensitive substances and there is a risk of solvent residue.

[0006] Supercritical CO2 extraction: no solvent residue, low temperature and high efficiency, but high equipment investment cost (>5 million yuan) makes it difficult to scale up.

[0007] Conventional chemical transesterification for the preparation of diester oil requires high temperatures (>80℃) and strong base catalysts, which leads to the formation of trans fatty acids and incomplete hydrolysis of triglycerides, resulting in low yields and a lack of selective synthesis. Summary of the Invention

[0008] In view of this, the purpose of this invention is to provide a method for preparing tiger nut diester oil. This invention solves the problems of low extraction rate, poor selectivity and high pollution by using magnetic field-light field synergistic regulation, and provides an efficient, precise and sustainable solution for the manufacture of functional diester oil.

[0009] To achieve the above objectives, the present invention provides the following technical solution:

[0010] This invention provides a method for preparing tiger nut diester oil, comprising the following steps:

[0011] S1. After drying and pulverizing tiger nuts, add PBS buffer and pectinase complex particles, and then perform enzymatic hydrolysis under an alternating magnetic field to obtain the enzymatic hydrolysate;

[0012] S2. The enzymatic hydrolysate is used to recover pectinase complex particles by magnetic separation, and then the supernatant oil is collected after centrifugation to obtain tiger nut oil;

[0013] S3. The tiger nut oil, glycerol, and lipase complex particles are then subjected to photo-magnetic fixation catalysis and magnetic separation to recover the lipase complex particles and obtain tiger nut diester oil.

[0014] First, the present invention involves drying and pulverizing tiger nuts, adding PBS buffer and pectinase complex particles, and then enzymatically hydrolyzing them under an alternating magnetic field to obtain an enzymatic hydrolysate.

[0015] In some embodiments, the moisture content of the dried tiger nuts is 5-8%, and the mesh size of the tiger nuts is 50-100 mesh. The drying conditions described above facilitate the subsequent release and separation of oils, and a certain mesh size allows the tiger nut powder to fully contact the pectinase complex particles.

[0016] In this invention, the mass ratio of tiger nuts, PBS buffer, and pectinase complex particles in S1 is 100:(300-500):(1-5).

[0017] In this invention, the preparation method of the pectinase complex particles is as follows:

[0018] Triton X-100, n-hexanol, and cyclohexane were mixed and ultrasonically emulsified for 10 minutes. Then, a dispersion of nano-Fe3O4 particles and ammonia were added, and the mixture was stirred to form a homogeneous microemulsion. Tetraethyl orthosilicate was added dropwise, and the mixture was reacted at 25°C for 12 hours. Fe3O4@SiO2 was collected by magnetic separation, washed, and dried.

[0019] Fe3O4@SiO2 was dispersed in 1 mg / mL toluene and sonicated for 30 minutes; 5 wt% of 3-aminopropyltriethoxysilane was added and refluxed at 80 °C for 6 hours; after magnetic separation and washing, aminated Fe3O4@SiO2-NH2 was obtained.

[0020] Fe3O4@SiO2-NH2 was dispersed in sodium citrate buffer, and then CBD protein was added to react. After the reaction was completed, the reaction was terminated, and pectin lyase solution was added and shaken at 4°C for 4 hours. After magnetic separation and washing, pectinase complex particles were obtained.

[0021] Further optimization yielded a ratio of Triton X-100, n-hexanol, cyclohexane, nano-Fe3O4 particles, ammonia, and tetraethyl orthosilicate of 10mL:10mL:40mL:(1-5)mg:0.5mL:0.3mL.

[0022] In some embodiments, the pectinase complex particles have an activity ≥300 U / mg and a particle size of 50-60 nm.

[0023] The Fe3O4@SiO2 core-shell structure in the pectinase composite particles of this invention is the key carrier. The magnetic core (Fe3O4) enables magnetic drive (directional penetration), magnetocaloric effect (local heating), and magnetic separation and recovery. The silica shell (SiO2) protects the magnetic core and provides a stable, biocompatible surface for functionalization. The cellulose-binding domain (CBD) specifically recognizes and tightly binds to cellulose, the main component of tiger nut cell walls, "anchoring" the nanoparticles to the cell walls. The pectin lyase specifically degrades the pectin layer connecting cellulose / hemicellulose in the cell walls, disrupting the cell wall structure. The combination of magnetically driven penetration, enzymatic cell wall disruption, and local thermal activation is crucial for efficient oil extraction. Moreover, the 45℃ temperature is far lower than that of solvent extraction or high-temperature pressing, protecting the quality of the oil.

[0024] In this invention, the alternating magnetic field parameters in S1 are 100-150kHz, 50-60mT, the processing temperature is 45-50℃, and the time is 20-40min.

[0025] This invention uses an alternating magnetic field to induce high-frequency mechanical vibrations in nanoparticles, directly acting on the cellulose microfibrils in the cell wall and disrupting the hydrogen bond network. CBD, through targeted anchoring, specifically binds to the cellulose chain via its cellulose binding domain. Magnetothermal heating to 45°C activates pectinase activity, enzymatically hydrolyzing the pectin layer to produce galacturonic acid oligomers, thereby effectively releasing tiger nut oil.

[0026] Secondly, the present invention recovers pectinase complex particles from the enzymatic hydrolysate through magnetic separation, and then collects the supernatant oil after centrifugation to obtain tiger nut oil.

[0027] In this invention, the centrifugation parameters are 12000-15000 rpm and the centrifugation time is 10-30 min.

[0028] The magnetic separation method of this invention can effectively separate pectinase complex particles, and the structure can retain enzyme activity for repeated use.

[0029] Finally, the present invention uses the tiger nut oil, glycerol, and lipase complex particles to obtain tiger nut diester oil by photo-magnetic fixation catalysis and magnetic separation to recover the lipase complex particles.

[0030] The existing methods for synthesizing tiger nut diester oil from tiger nut oil and glycerol using lipases have the following problems: the product yield is low, and there is a lack of selective synthesis. The yield of 1,3-diester oil is only about 50%. Moreover, a pH adjuster needs to be added during synthesis, which not only increases the cost but also contains components that are harmful to human health. At the same time, the reaction endpoint is difficult to control and is prone to over-catalysis, which produces impurities.

[0031] In this invention, the mass ratio of tiger nut oil, glycerol, and lipase composite particles is 3:1:(0.01-0.03).

[0032] In this invention, the method for preparing the lipase complex particles is as follows:

[0033] 1) Mix ZrOCl2·8H2O, tetrakis(4-carboxyphenyl)porphyrin, benzoic acid, and N,N-dimethylformamide and sonicate to dissolve them. Then react at 80-85℃ for 18-24 hours and filter to obtain material A.

[0034] 2) Material A is washed, dried, and activated to obtain material B;

[0035] 3) After mixing CALB lipase, phosphate buffer, and material B, the mixture was subjected to vacuum adsorption to obtain material C;

[0036] 4) The material C, 1-ethyl-(3-dimethylaminopropyl)carbodiimide, N-hydroxysuccinimide, CALB lipase, and phosphate buffer were shaken and reacted. The pH was then adjusted to 5, ethanolamine was added, and the solid was collected by centrifugation, washed, and dried to obtain lipase composite particles.

[0037] In this invention, the ratio of ZrOCl2·8H2O, tetrakis(4-carboxyphenyl)porphyrin, benzoic acid, and N,N-dimethylformamide in 1) is (75-85) mg:(40-50) mg:(1.5-1.8) g:(15-30) mL.

[0038] In this invention, the activation temperature in step 2) is (130-150)℃ and the time is (18-24)h.

[0039] In this invention, the ratio of CALB lipase, phosphate buffer, and material B in step 3) is (30-50) mg: 10 mL: 100 mg.

[0040] In this invention, the ratio of material C, 1-ethyl-(3-dimethylaminopropyl)carbodiimide, N-hydroxysuccinimide, CALB lipase, and phosphate buffer in step 4) is 100mg:(1-5)mg:(2-10)mg:10mg:10mL.

[0041] In some embodiments, the lipase complex particles have a pore size distribution of 3.8 ± 0.2 nm and a specific surface area > 2200 m². 2 / g.

[0042] In some embodiments, the lipase complex granule enzyme activity is ≥1000 U / g.

[0043] The lipase complex particles and carrier (PCN-224) of this invention, with porphyrin groups attached, endow them with excellent photosensitivity. The carefully designed pore size (3.8 nm) allows small glycerol molecules to freely enter and participate in the reaction, but may limit the diffusion of large substrates or products, thus improving selectivity for the target product. Simultaneously, it enables reaction start-stop control, avoiding over-reaction or byproduct formation.

[0044] In this invention, the parameters of the photo-magnetic fixed catalysis are:

[0045] Static magnetic field: 50mT fixed catalyst;

[0046] NIR laser: 808nm, 1.5W / cm 2 Pulse mode, on for 5 minutes / off for 1 minute;

[0047] Reaction conditions: 45℃, 200rpm, 2h.

[0048] This invention utilizes the pore size sieving effect of lipase composite particles. The precisely designed 3.8nm pore size allows glycerol to freely enter and exit while blocking 1,2-diester diffusion and inhibiting acyl migration. Furthermore, it employs photo-controlled remodeling of the enzyme's active site; near-infrared light (808nm) induces reactive oxygen species in porphyrins, which then locally generate H+. + The increased concentration caused the nearby pH to drop to 5.5, initiating a catalytic enzymatic hydrolysis reaction.

[0049] Simultaneously, when the laser is turned on, the local pH is ≤5.5, the enzyme active center opens, and the reaction proceeds; when the laser is turned off, the environmental buffer system diffuses the pH back to 7.0, the enzyme conformation closes, and the reaction terminates. Thus, no other conditions need to be controlled, reducing costs and procedures, and it can also effectively inhibit excessive catalytic side reactions.

[0050] It contains at least the following beneficial technical effects:

[0051] This invention achieves efficient and green preparation of tiger nut diester oil through dual innovations of magnetically controlled nanoenzyme targeted cell wall disruption and photo-magnetic synergistic intelligent catalysis. An alternating magnetic field drives pectinase composite particles to penetrate the cell wall in a directional manner, combined with magnetothermal triggering of enzyme activity (45℃), increasing the oil extraction rate to 90% with no solvent contamination throughout the process. The photoresponsive MOF carrier (lipase composite particles) activates the specificity of enzyme Sn-1,3 through near-infrared light-controlled pH microenvironment (pH 7.0→5.5), combined with 3.8nm pore size sieving to inhibit acyl migration, achieving 88% selectivity for 1,3-diesters and a total diester yield >90%. Pulsed light irradiation enables self-termination of the reaction, avoiding over-catalysis and impurities. The magnetic carrier is recyclable, allowing for high-value product utilization, with no chemical additive residues, meeting the safety standards for functional oils. Detailed Implementation

[0052] Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention. The invention will now be described with reference to specific embodiments. It should be noted that these embodiments are merely descriptive and do not limit the invention in any way.

[0053] Raw material source:

[0054] Pectinase complex particles were prepared in-house using the following method:

[0055] Triton X-100, n-hexanol, and cyclohexane were mixed and ultrasonically emulsified for 10 minutes. Then, a dispersion of nano-Fe3O4 particles and ammonia were added and stirred to form a homogeneous microemulsion. Tetraethyl orthosilicate was added dropwise and reacted at 25°C for 12 hours. Fe3O4@SiO2 was collected by magnetic separation, washed, and dried. The ratio of Triton X-100, n-hexanol, cyclohexane, nano-Fe3O4 particles, ammonia, and tetraethyl orthosilicate was 10 mL: 10 mL: 40 mL: (1-5) mg: 0.5 mL: 0.3 mL.

[0056] Fe3O4@SiO2 was dispersed in 1 mg / mL toluene and sonicated for 30 minutes; 5 wt% of 3-aminopropyltriethoxysilane was added and refluxed at 80 °C for 6 hours; after magnetic separation and washing, aminated Fe3O4@SiO2-NH2 was obtained.

[0057] Fe3O4@SiO2-NH2 was dispersed in sodium citrate buffer, and then 5 mg of CBD protein was added and reacted for 12 h. The reaction was then terminated, and 10 mg of pectinase solution was added and shaken at 4 °C for 4 h. After magnetic separation and washing, pectinase composite particles were obtained. The activity of the pectinase composite particles was 348 U / mg, and the particle size was 57 nm.

[0058] Lipase complex particles were prepared in-house using the following method:

[0059] 1) Mix ZrOCl2·8H2O, tetrakis(4-carboxyphenyl)porphyrin, benzoic acid, and N,N-dimethylformamide and dissolve by sonication. Then react at 85℃ for 24 hours and filter to obtain material A. The ratio of ZrOCl2·8H2O, tetrakis(4-carboxyphenyl)porphyrin, benzoic acid, and N,N-dimethylformamide is 80mg:45mg:1.6g:30mL.

[0060] 2) Wash, dry, and activate material A at 150℃ for 24 hours to obtain material B;

[0061] 3) After mixing CALB lipase, phosphate buffer, and material B, the mixture was subjected to vacuum adsorption to obtain material C; the ratio of CALB lipase, phosphate buffer, and material B was 40 mg: 10 mL: 100 mg.

[0062] 4) The material C, 1-ethyl-(3-dimethylaminopropyl)carbodiimide, N-hydroxysuccinimide, CALB lipase, and phosphate buffer were shaken and reacted. The pH was then adjusted to 5, ethanolamine was added, and the solid was collected by centrifugation, washed, and dried to obtain lipase composite particles. The ratio of material C, 1-ethyl-(3-dimethylaminopropyl)carbodiimide, N-hydroxysuccinimide, CALB lipase, and phosphate buffer was 100 mg: 5 mg: 10 mg: 10 mg: 10 mL.

[0063] The lipase complex particles were found to have a pore size distribution of 3.8 ± 0.2 nm and a specific surface area of ​​2381 m². 2 / g, the enzyme activity of the lipase complex granules is 1087U / g.

[0064] Tiger nuts are dried to a moisture content of 7% and then crushed and passed through an 80-mesh sieve.

[0065] All other raw materials can be sourced from commercially available sources.

[0066] Example 1

[0067] S1. After drying and pulverizing tiger nuts, add PBS buffer and pectinase complex particles, and then perform enzymatic hydrolysis under an alternating magnetic field to obtain the enzymatic hydrolysate; wherein, the mass ratio of tiger nuts, PBS buffer and pectinase complex particles is 100:400:3; the alternating magnetic field parameters are 130kHz, 55mT, the treatment temperature is 48℃ and the time is 30min.

[0068] S2. The enzymatic hydrolysate is magnetically separated to recover pectinase complex particles, and then centrifuged at 13000 rpm for 20 min to collect the supernatant oil to obtain tiger nut oil.

[0069] S3. The tiger nut oil, glycerol, and lipase composite particles are then subjected to photo-magnetic immobilization catalysis and magnetic separation to recover the lipase composite particles, yielding tiger nut diester oil; wherein the mass ratio of tiger nut oil, glycerol, and lipase composite particles is 3:1:0.02; the parameters and reaction conditions for the photo-magnetic immobilization catalysis are as follows:

[0070] Static magnetic field: 50mT fixed catalyst;

[0071] NIR laser: 808nm, 1.5W / cm 2 Pulse mode, on for 5 minutes / off for 1 minute;

[0072] Reaction conditions: 45℃, 200rpm, 2h.

[0073] Example 2

[0074] S1. After drying and pulverizing tiger nuts, add PBS buffer and pectinase complex particles, and then perform enzymatic hydrolysis under an alternating magnetic field to obtain the enzymatic hydrolysate; wherein, the mass ratio of tiger nuts, PBS buffer and pectinase complex particles is 100:300:1; the alternating magnetic field parameters are 100kHz, 50mT, the treatment temperature is 45℃ and the time is 20min.

[0075] S2. The enzymatic hydrolysate is magnetically separated to recover pectinase complex particles, and then centrifuged at 13000 rpm for 10 min to collect the supernatant oil to obtain tiger nut oil.

[0076] S3. The tiger nut oil, glycerol, and lipase composite particles are then subjected to photo-magnetic immobilization catalysis and magnetic separation to recover the lipase composite particles, yielding tiger nut diester oil; wherein the mass ratio of tiger nut oil, glycerol, and lipase composite particles is 3:1:0.01; the parameters and reaction conditions for the photo-magnetic immobilization catalysis are as follows:

[0077] Static magnetic field: 50mT fixed catalyst;

[0078] NIR laser: 808nm, 1.5W / cm 2 Pulse mode, on for 5 minutes / off for 1 minute;

[0079] Reaction conditions: 45℃, 200rpm, 2h.

[0080] Example 3

[0081] S1. After drying and pulverizing tiger nuts, add PBS buffer and pectinase complex particles, and then perform enzymatic hydrolysis under an alternating magnetic field to obtain the enzymatic hydrolysate; wherein, the mass ratio of tiger nuts, PBS buffer and pectinase complex particles is 100:500:5; the alternating magnetic field parameters are 150kHz, 60mT, the treatment temperature is 50℃ and the time is 40min.

[0082] S2. The enzymatic hydrolysate is magnetically separated to recover pectinase complex particles, and then centrifuged at 15000 rpm for 30 min to collect the supernatant oil to obtain tiger nut oil.

[0083] S3. The tiger nut oil, glycerol, and lipase composite particles are then subjected to photo-magnetic immobilization catalysis and magnetic separation to recover the lipase composite particles, yielding tiger nut diester oil; wherein the mass ratio of tiger nut oil, glycerol, and lipase composite particles is 3:1:0.03; the parameters and reaction conditions for the photo-magnetic immobilization catalysis are as follows:

[0084] Static magnetic field: 50mT fixed catalyst;

[0085] NIR laser: 808nm, 1.5W / cm 2 Pulse mode, on for 5 minutes / off for 1 minute;

[0086] Reaction conditions: 45℃, 200rpm, 2h.

[0087] Comparative Example 1 (Oil Extraction Problem)

[0088] The method of Comparative Example 1 is the same as that of Example 1, except that no magnetic field is applied.

[0089] Comparative Example 2 (Oil Extraction Issues)

[0090] The method of Comparative Example 1 is the same as that of Example 1, except that CBD protein is not added to directly load pectin lyase when preparing pectinase complex particles.

[0091] Comparative Example 3 (Traditional Enzymatic Hydrolysis)

[0092] The method of Comparative Example 1 is the same as that of Example 1, except that the lipase complex particles are replaced with CALB lipase with an enzyme activity of 10000 U / g and the total enzyme activity is made close; the enzymatic hydrolysis catalysis parameters are 45°C and the reaction is carried out for 8 hours.

[0093] Comparative Example 4 (1.3 purity)

[0094] The method of Comparative Example 1 is the same as that of Example 1, except that the pore size of the lipase complex particles is 10 nm.

[0095] Experimental Example 1

[0096] Tiger nut oil extraction detection

[0097] The oil extraction rates of tiger nuts in Examples 1-3 and Comparative Examples 1-2 were analyzed, as shown in Table 1.

[0098] Table 1 Oil Extraction Rate

[0099] Oil extraction rate % Example 1 94 Example 2 98 Example 3 92 Comparative Example 1 76 Comparative Example 2 81

[0100] As shown in Table 1, the oil extraction rates of Examples 1-3 in this invention are relatively high, and compared with traditional extraction methods, they have advantages such as low temperature, non-toxicity, greenness, low cost, and high extraction rate. It also shows that the preparation of the pectinase composite particles greatly promotes the release of oil under the physical vibration of the magnetic field and the adsorption of CBD.

[0101] Experiment Example 2

[0102] The yield and types of tiger glycerol diglycerides in Examples 1-3 and Comparative Examples 3-4 were analyzed, as shown in Table 2.

[0103] Table 2

[0104] Diglyceride yield % 1,3-Diglycerides (as a percentage of total diglycerides) Example 1 91 89 Example 2 88 85 Example 3 92 87 Comparative Example 3 67 49 Comparative Example 4 86 67

[0105] As shown in Table 2, the catalytic enzymatic hydrolysis method of the present invention has the characteristics of selectively synthesizing 1,3-diglycerides, and the total yield of diglycerides is high. It does not require chemical regulation, reduces the use of reagents, and the reaction degree is controllable, effectively inhibiting excessive reaction and reducing the by-products it brings.

[0106] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A process for the preparation of chufa distillate oil, characterized in that, The method comprises the following steps: S1. After the Cyperus esculentus is dried and crushed, the Cyperus esculentus is added into a PBS buffer and a pectinase composite particle, and then enzymolysis is performed under an alternating magnetic field to obtain an enzymolysis liquid; S2. The pectinase composite particle in the enzymolysis liquid is recovered through magnetic separation, and then the supernatant oil is collected through centrifugation to obtain Cyperus esculentus oil; S3. The Cyperus esculentus oil, glycerol and a lipase composite particle are subjected to light-magnetic fixed catalysis and magnetic separation to recover the lipase composite particle, so as to obtain Cyperus esculentus diester oil; The preparation method of the pectinase composite particle is as follows: Triton X-100, n-hexanol and cyclohexane are mixed and ultrasonically emulsified for 10 minutes; then nano Fe3O4 particle dispersion liquid and ammonia water are added to form a uniform microemulsion; tetraethyl orthosilicate is added dropwise, and reaction is performed at 25 DEG C for 12 hours; Fe3O4@SiO2 is collected through magnetic separation and then washed and dried; Fe3O4@SiO2 is dispersed in 1 mg / mL toluene and ultrasonically treated for 30 minutes; 5wt% 3-aminopropyltriethoxysilane is added, and reflux is performed at 80 DEG C for 6 hours; after magnetic separation, washing is performed to obtain amino Fe3O4@SiO2-NH2; Fe3O4@SiO2-NH2 is dispersed in a sodium citrate buffer solution, and then CBD protein is added for reaction; after the reaction is completed, the reaction is terminated, and pectin lyase solution is added for oscillation at 4 DEG C for 4 hours; after magnetic separation and washing, a pectinase composite particle is obtained; The proportions of Triton X-100, n-hexanol, cyclohexane, nano Fe3O4 particle, ammonia water and tetraethyl orthosilicate are 10 mL:10 mL:40 mL:(1-5) mg:0.5 mL:0.3 mL; The preparation method of the lipase composite particle in S3 is as follows: 1) ZrOCl2.8H2O, tetra(4-carboxyphenyl) porphyrin, benzoic acid and N,N-dimethylformamide are mixed and ultrasonically dissolved, and then reaction is performed at 80-85 DEG C for 18-24 hours; then material A is obtained through filtration; 2) Material A is washed, dried and activated to obtain material B; 3) CALB lipase, phosphate buffer solution and material B are mixed to perform vacuum adsorption to obtain material C; 4) Material C, 1-ethyl-(3-dimethylaminopropyl) carbodiimide, N-hydroxysuccinimide, CALB lipase and phosphate buffer solution are oscillated to react, and then ethanolamine is added after the pH is adjusted to 5; then the solid is collected through centrifugation, washed and dried to obtain a lipase composite particle; In 1), the proportions of ZrOCl2.8H2O, tetra(4-carboxyphenyl) porphyrin, benzoic acid and N,N-dimethylformamide are (75-85) mg:(40-50) mg:(1.5-1.8) g:(15-30) mL; In 2), the activation temperature is (130-150) DEG C, and the time is (18-24) h; In 3), the proportions of CALB lipase, phosphate buffer solution and material B are (30-50) mg:10 mL:100 mg; The ratio of the 4) material C, 1-ethyl-(3-dimethylaminopropyl) carbodiimide, N-hydroxysuccinimide, CALB lipase, phosphate buffer is 100 mg: (1-5) mg: (2-10) mg: 10 mg: 10 mL; The parameters of the photo-magnetic immobilized catalysis are: Magnetostatic field: 50 mT immobilized catalyst; NIR laser: 808 nm, 1.5 W / cm 2 , pulse mode, on 5 min / off 1 min; Reaction condition: 45℃, 200 rpm, 2h.

2. The production method according to claim 1, characterized by, The mass ratio of the S1 sedge, PBS buffer, pectinase composite particles is 100: (300-500): (1-5).

3. The preparation method according to claim 1, characterized in that, The mass ratio of the S3 sedge oil, glycerol, lipase composite particles is 3:1: (0.01-0.03). The mass ratio of the S1 sedge, PBS buffer, pectinase composite particles is 100: (300-500): (1-5). The mass ratio of the S3 sedge oil, glycerol, lipase composite particles is 3:1: (0.01-0.03).