Sample slice pasting device and method

Abstract

The invention provides a sample slice pasting device and method, and belongs to the technical field of slice pasting. The sample slice pasting device comprises a sample water tank, a first connecting structure, a second connecting structure, a first pipeline, a second pipeline, a third pipeline, a fourth pipeline, a fifth pipeline, a first valve, a second valve, a detection piece, a glass slide, a first water pump and a second water pump. According to the invention, the first water pump, the second water pump, the first valve and the second valve are controlled in order, the sheet sample in the sample water tank is stably conveyed into the second pipeline, and the second valve is opened, so that the protection solution and the sheet sample in the second pipeline fall onto the carrier slide under the action of gravity; according to the automatic slicing device, slice samples generated in the automatic slicing process can be collected and automatically pasted on a glass slide, the manual repeated operation time is saved, and the problem that sample slices are polluted due to manual operation errors is solved.

Description

Technical Field

[0001] This invention relates to the field of sheet mounting technology, and more specifically, to a sheet mounting apparatus and method for sample sheets. Background Technology

[0002] In existing sample mounting technology, sample sheets are transported via waterways, manually transferred using tweezers, and placed on the water. Once the sample sheets are flattened on the water, they are then mounted. However, this existing technology has the following problems: it requires manual placement of the sample sheets on the water before mounting, making it impossible to use automatically cut sample sheets for continuous, automated mounting; the sample sheets are easily folded or torn during manual transfer due to tweezers or brushes, and are also susceptible to contamination if the tweezers, brushes, and other tools are not thoroughly cleaned.

[0003] Chinese Patent CN119574264A, published on 2025-03-07, discloses a pathological slide mounting device and its usage method, relating to the field of medical equipment technology. The device includes a processing frame with a resistance heating plate installed at the bottom of its inner cavity, and a fine-tuning mechanism comprising a mounting frame, a moving rod, and a mounting ring. The mounting frame is located at the upper end of the inner cavity of the processing frame, and the mounting ring is located at the bottom of the mounting frame. The moving rod is installed at the right end of the mounting ring. This invention increases the efficiency and stability of the slide mounting process by applying negative pressure around the mounting area. It avoids situations where the slide pushes away water at the top of the slide as it moves upwards, creating a water flow that could guide the slide to another position, leading to unsuccessful mounting. In the aforementioned patent, the slide is moved upward in a liquid water environment to get closer to the slice and then attached. The slice is attached by finely adjusting its position. However, the slice needs to be placed on the water manually to make it spread out flat. It cannot be attached with slices cut off in real time. The process requires repetitive manual operation and is inefficient.

[0004] Chinese Patent CN105842000B, published on June 22, 2018, discloses an automatic slice collection device and method for vibratory microtome. The device includes: a water tank containing a buffer solution; a vibratory microtome disposed within the water tank; a first flexible tube, one end of which faces the slice cut by the vibratory microtome, and the other end connected to a water pump with a forward and reverse motor; a first valve on the first tube, and a filter screen between the water pump and the first tube; and a second tube, one end of which connects to the first valve on the first tube and the water pump, and a second valve on the second tube. This invention enables automatic collection of sample slices during vibratory microtome processing without manual intervention, improving work efficiency. Furthermore, the device has a simple structure and is easily adaptable to different vibratory microtome machines. In the aforementioned patent, the transport of slices in the water circuit is controlled by the cooperation of a water pump and valves, and the flow of slices into the water pump is blocked by a filter screen. However, when the slices are adsorbed onto the filter screen by the water flow driven by the water pump, there will still be an adsorption force, which can easily lead to the breakage of the slices. At the same time, it is impossible to confirm whether the slices have passed through the pipeline, and it is impossible to confirm whether there are multiple slices in the water circuit that affect the dispensing and collection of slices. Furthermore, the water circuit device cannot control the falling posture of the slices to ensure that they are flatly attached to the glass slide, resulting in poor attachment effect. Summary of the Invention

[0005] To address the aforementioned issues, embodiments of this application provide a sample sheet mounting apparatus and method.

[0006] In a first aspect, this application provides a sample slide mounting device, including a sample water tank, a first connecting structure, a second connecting structure, a first pipe, a second pipe, a third pipe, a fourth pipe, a fifth pipe, a first valve, a second valve, a detection element, a glass slide, a first water pump, and a second water pump; both the first and second connecting structures are structures for conveying liquid, and both the first connecting structure and the second interface structure include multiple interfaces communicating with their interiors; the second pipe allows liquid to enter its interior from one end and flow out from its other end under gravity; the sample water tank is used to store a protective solution and a sample slide respectively; the first valve is disposed on the first pipe, and the second valve... The first pipe is installed on the second pipe; one end of the first pipe extends into the protective solution of the sample bath, and the other end is connected to the first interface of the first connecting structure; one end of the second pipe is connected to the first interface of the second connecting structure; the glass slide is installed below the second pipe and at a position matching the other end of the second pipe; the two ends of the third pipe are respectively connected to the second interface of the first connecting structure and the second interface of the second connecting structure; the detection element is installed on the third pipe for detecting the thin-section sample; the two ends of the fourth pipe are respectively connected to the third interface of the second connecting structure and the input end of the first water pump; the two ends of the fifth pipe are respectively connected to the third interface of the first connecting structure and the input end of the second water pump.

[0007] Preferably, the third pipe is inclined upward along the direction from one end of the third pipe connected to the first connecting structure to the other end connected to the second connecting structure, and / or the second interface of the second connecting structure is inclined upward along the direction from one end of the second interface of the second connecting structure connected to the third pipe to the center of the second connecting structure.

[0008] Preferably, the first water pump is a diaphragm pump.

[0009] Preferably, the second interface of the second connecting structure is lower than the first interface of the second connecting structure along the direction of gravity.

[0010] Preferably, it also includes a tray and an adjusting member, wherein the glass slides are multiple slides respectively disposed on the tray; the adjusting member is connected to the tray and is used to drive the tray to move, so that the tray drives the target glass slide to a position that matches the other end of the second pipe.

[0011] Preferably, the system also includes a collection tank and a sixth pipe. The collection tank is used to store the protective solution. One end of the sixth pipe is connected to the output end of the first water pump, and the other end is connected to the collection tank, so as to transport the protective solution discharged by the first water pump to the collection tank.

[0012] Preferably, it also includes a seventh pipe, one end of which is connected to the output end of the second water pump and the other end is connected to the collection tank, for conveying the protective solution discharged by the second water pump to the collection tank.

[0013] Preferably, the detection element includes a photoelectric sensor, and the line connecting the transmitting end and the receiving end of the photoelectric sensor intersects the axis of the third pipe.

[0014] Preferably, there are multiple photoelectric sensors.

[0015] Preferably, it also includes a conveyor for extracting and conveying the protective solution in the collection tank to the sample tank.

[0016] Preferably, it also includes an eighth pipe and a third water pump, wherein the two ends of the eighth pipe are respectively connected to the input end of the third water pump and the fourth interface of the second connection structure.

[0017] Preferably, the conveying component includes a ninth pipe, a tenth pipe, and a fourth water pump. The two ends of the ninth pipe are respectively connected to the liquid collection tank and the input end of the fourth water pump, and the two ends of the tenth pipe are respectively connected to the output end of the fourth water pump and the sample tank.

[0018] Secondly, embodiments of this application provide a method for attaching a sample sheet, comprising the following steps:

[0019] S1: Open the first valve and turn on the first water pump. The first water pump extracts the protective solution from the sample tank, so that the protective solution flows sequentially to the first pipe, the first connecting structure, the third pipe, and the second connecting structure. The thin film sample flows with the protective solution.

[0020] S2: When the test piece detects a sheet sample, the first water pump is turned off, the first valve is closed, and the second valve is opened, so that the protective solution in the second pipe falls down under the action of gravity and is discharged out of the second pipe;

[0021] S3: Close the second valve and turn on the second water pump to allow the protective solution in the third pipe to flow into the second connection structure. The protective solution carries the thin film sample into the second pipe.

[0022] S4: Turn off the second water pump and place the glass slide below the second pipe at a position that matches the other end of the second pipe;

[0023] S5: Open the second valve so that the protective solution and the thin-film sample in the second pipe fall onto the glass slide under the action of gravity.

[0024] The beneficial effects of this invention are as follows: A detection element is installed on the third pipe to detect the thin-slice sample, thereby effectively determining whether the thin-slice sample is inside the third pipe; the addition of multiple pipes, multiple connecting structures, and multiple water pumps allows for the orderly control of the first water pump, the second water pump, the first valve, and the second valve, stably transporting the thin-slice sample located in the sample tank to the second pipe. When the glass slide is positioned below the second pipe and matches the other end of the pipe, the second valve is opened, allowing the protective solution and the thin-slice sample in the second pipe to fall onto the glass slide under gravity. This enables the collection and mounting of the thin-slice sample generated during the automatic slicing process. The thin-slice sample is kept in the protective solution throughout the entire process. Position control and gravity collection via multiple pumps avoid impact breakage caused by interception of the thin-slice sample using filters or other components; it also eliminates the need for manual tweezers to pick up the thin-slice sample or a brush to push it, saving time spent on repetitive manual operations and avoiding sample contamination due to human error. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1This is a schematic diagram of the structure of a sample sheet patching device provided in an embodiment of this application;

[0027] Figure 2 This is a schematic diagram of the structure of a sample slide mounting device provided in an embodiment of this application, in which the adjusting component is connected to the tray, the second pipe, and the target glass slide in a corresponding arrangement;

[0028] Figure 3 A schematic diagram of the structure of a sample sheet patching device provided in this application embodiment, wherein the detection element is disposed in the third channel;

[0029] Figure 4 This is a schematic flowchart illustrating a method for attaching a sample sheet according to an embodiment of this application.

[0030] In the diagram: 1-First water pump, 2-Second water pump, 3-Third water pump, 4-First valve, 5-Second valve, 6-Second connecting structure, 7-Detection piece, 8-Slide, 9-First pipe, 10-Second pipe, 11-Sixth pipe, 12-Seventh pipe, 13-Thin sample, 14-Protective solution, 15-Sample tank, 16-Collection tank, 18-Fifth pipe, 19-Eighth pipe, 20-Third pipe, 21-Fourth pipe, 22-Tenth pipe, 23-Ninth pipe, 24-Fourth water pump, 25-First connecting structure, 26-Adjusting piece, 27-Tray, 28-Photoelectric sensor. Detailed Implementation

[0031] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings.

[0032] In the following description, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. The following description provides multiple embodiments of this application, which can be substituted or combined with each other. Therefore, this application can also be considered to include all possible combinations of the same and / or different embodiments described. Thus, if one embodiment includes features A, B, and C, and another embodiment includes features B and D, then this application should also be considered to include embodiments containing one or more other possible combinations of A, B, C, and D, even if such embodiments are not explicitly described in the following text.

[0033] The following description provides examples and does not limit the scope, applicability, or examples set forth in the claims. Changes may be made to the function and arrangement of the described elements without departing from the scope of this application. Various processes or components may be appropriately omitted, substituted, or added to the examples. For example, the described methods may be performed in a different order than described, and various steps may be added, omitted, or combined. Furthermore, features described with respect to some examples may be combined into other examples.

[0034] Please see Figure 1-4 . Figure 1 This is a schematic diagram of the structure of a sample sheet patching device provided in an embodiment of this application. Figure 2 This is a schematic diagram of the structure of a sample slide mounting device provided in this application, in which the adjusting member 26 is connected to the tray 27, the second pipe 10, and the target glass slide 8, respectively. Figure 3 This is a schematic diagram of the structure of a sample sheet patching device provided in this application, in which the detection element 7 is disposed in the third pipe 20. Figure 4 This is a schematic flowchart illustrating a method for attaching a sample sheet according to an embodiment of this application. In this embodiment, the apparatus includes a sample tank 15, a first connecting structure 25, a second connecting structure 6, a first pipe 9, a second pipe 10, a third pipe 20, a fourth pipe 21, a fifth pipe 18, a first valve 4, a second valve 5, a detection element 7, a glass slide 8, a first water pump 1, and a second water pump 2. Both the first connecting structure 25 and the second connecting structure 6 are structures for transporting liquids. Both the first connecting structure 25 and the second connecting structure 6 include multiple interfaces communicating with their interiors. The second pipe 10 allows liquid to enter from one end and flow out from the other end under gravity. The sample tank 15 stores a protective solution 14 and a sample sheet 13, respectively. The first valve 4 is disposed on the first pipe 9, and the second valve 5 is disposed on the second pipe. 10; one end of the first pipe 9 extends into the protective solution 14 of the sample water tank 15, and the other end is connected to the first interface of the first connecting structure 25; one end of the second pipe 10 is connected to the first interface of the second connecting structure 6; the glass slide 8 is disposed below the second pipe 10 and at a position matching the other end of the second pipe 10; the two ends of the third pipe 20 are respectively connected to the second interface of the first connecting structure 25 and the second interface of the second connecting structure 6; the detection element 7 is disposed on the third pipe 20 for detecting the thin-section sample 13; the two ends of the fourth pipe 21 are respectively connected to the third interface of the second connecting structure 6 and the input end of the first water pump 1; the two ends of the fifth pipe 18 are respectively connected to the third interface of the first connecting structure 25 and the input end of the second water pump 2.

[0035] In this embodiment, all pumps and valves are closed at the start of patch application. A protective solution 14 is stored in the sample tank 15, and the sheet sample 13 is stored in the sample tank 15 and immersed in the protective solution 14. First, the first valve 4 and the first pump 1 are opened. The first pump 1 draws the protective solution 14 from the sample tank 15 into the first pipe 9. Under the action of the first pump 1, the protective solution 14 in the first pipe 9 continues to flow, sequentially flowing to the first connecting structure 25, the third pipe 20, and the second connecting structure 6. As the protective solution 14 flows in the first pipe 9, it causes the sheet sample 13 to move. The sheet sample 13 can flow sequentially with the protective solution 14 to the first pipe 9, the first connecting structure 25, the third pipe 20, and the second connecting structure 6. A detection element 7 is provided on the first pipe 9. When the detection element 7 detects the sheet sample 13, it indicates that the sheet sample 13 has moved to a first preset position within the first pipe 9. At this time, the first pipe 9, the first connecting structure 25, the third pipe 20, the second connecting structure 6, and the second pipe 10 all contain the protective solution 14. The second pipe 10 allows liquid to enter from one end and flow out from the other end under gravity; that is, the second pipe is a tubular structure that allows liquid entering from one end to exit from the other end under gravity. When the protective solution 14 and / or the sheet sample 13 enter the interior of the second pipe 10 from one end, the protective solution 14 and / or the sheet sample 13 can flow out from the other end of the second pipe 10 under the action of gravity. Then, the first water pump 1 is turned off, the first valve 4 is turned off, and the second valve 5 is turned on. The protective solution 14 in the second pipe 10 falls and is discharged from the second pipe 10 under the action of gravity. Then, the second valve 5 is turned off and the second water pump 2 is turned on, so that the protective solution 14 in the third pipe 20 flows sequentially into the second connecting structure 6. The protective solution 14 carries the sheet sample 13 to move into the second pipe 10. Through the pressurization of the second water pump 2, the liquid column and The thin-film sample 13 compresses the air in the second pipe 10, allowing the protective solution 14 and the thin-film sample 13 to enter the second connecting structure 6. The protective solution 14 then moves the thin-film sample 13 into the second pipe 10. Next, the second water pump 2 is turned off, and the glass slide 8 is placed below the second pipe 10 and at a position matching the other end of the second pipe 10. During this period, the thin-film sample 13 in the second pipe 10 settles and expands, reducing the probability of the thin-film sample 13 folding or curling due to pressure movement in the protective solution 14. Finally, the second valve 5 is opened, allowing the protective solution 14 and the thin-film sample 13 in the second pipe 10 to fall onto the glass slide 8 under the action of gravity.This application adds a detection element 7 to the third pipeline 20, which can effectively determine the transport position of the thin film sample 13, avoid it from being crushed in the water pump, add multiple water pump pipelines, orderly control the water pump and valve switch, stably transport the thin film sample 13 to the corresponding pipeline, and automatically flatten and attach the thin film sample 13 to the glass slide 8.

[0036] In this embodiment, the first connecting structure 25 can be a tee pipe, with its three connection ports being the first interface, second interface, and third interface of the first connecting structure 25, respectively. The second connecting structure 6 can be a four-way pipe, with its four connection ports being the first interface, second interface, third interface, and fourth interface of the second connecting structure 6, respectively. The second connecting structure 6 may also include two tee pipes and a connecting pipe, with its two ends connected to the first connection port of the first tee pipe and the first connection port of the second tee pipe, respectively. The second and third connection ports of the first and second tee pipes serve as the first interface, second interface, third interface, and fourth interface of the second connecting structure 6, respectively.

[0037] The third pipe 20 may be inclined upward along the direction from one end connected to the first connecting structure 25 to the other end connected to the second connecting structure 6, and / or the second interface of the second connecting structure 6 may be inclined upward along the direction from one end connected to the third connecting structure 20 to the center of the second connecting structure 6.

[0038] In this embodiment, when the detection element 7 detects the sheet sample 13, the first water pump 1 is turned off, the first valve 4 is closed, and the second valve 5 is opened, so that the protective solution 14 in the second pipe 10 falls and is discharged from the second pipe 10 under the action of gravity. Since the third pipe 20 is inclined upward and / or the second interface of the second connecting structure 6 is inclined upward, the probability that the sheet sample 13 located in the third pipe 20 will be mistakenly discharged due to the Venturi effect during the process of the protective solution 14 in the second pipe 10 being discharged under the action of gravity can be reduced.

[0039] The first water pump 1 can be a diaphragm pump.

[0040] In this embodiment, when the detection element 7 detects the sheet sample 13, the first water pump 1 is turned off, the first valve 4 is turned off, and the second valve 5 is turned on, causing the protective solution 14 in the second pipe 10 to fall and be discharged outside the second pipe 10 under the action of gravity. Then, the second valve 5 is turned off and the second water pump 2 is turned on. The first water pump 1 is a diaphragm pump, so the diaphragm of the first water pump 1 is opened under pressure to release air, causing the protective solution 14 in the third pipe 20 to flow into the second connecting structure 6. The protective solution 14 carries the sheet sample 13 to move into the second pipe 10. When the detection element 7 detects the sheet sample 13, the first water pump 1 is turned off, the first valve 4 is turned off, and the second valve 5 is turned on, causing the protective solution 14 in the second pipe 10 to fall and be discharged outside the second pipe 10 under the action of gravity. The first water pump 1 is not a diaphragm pump. Then, the second valve 5 is turned off, the first water pump 1 is turned on, and the second water pump 2 is turned on, causing the protective solution 14 in the third pipe 20 to flow into the second connecting structure 6. The protective solution 14 carries the sheet sample 13 to move into the second pipe 10.

[0041] Along the direction of gravity, the second interface of the second connecting structure 6 is lower than the first interface of the second connecting structure 6.

[0042] In this embodiment, after the protective solution 14 passes through the second interface of the second connecting structure 6 and enters the interior of the second connecting structure 6, it flows to the first interface of the second connecting structure 6 under the action of gravity, and then passes through the first interface of the second connecting structure 6 into the second pipe 10. The protective solution 14 moves against the direction of gravity in the second connector structure 6, which is beneficial for controlling the position of the sheet sample 13 during the start-up of the second water pump 2.

[0043] As a sample slide mounting device in this embodiment, it may also include a tray 27 and an adjusting member 26. The glass slides 8 are multiple slides respectively disposed on the tray 27. The adjusting member 26 is connected to the tray 27 and is used to drive the tray 27 to move, so that the tray 27 drives the target glass slide 8 to a position that matches the other end of the second pipe 10.

[0044] In this embodiment, the adjusting member 26 can drive the tray 27 to move linearly along a first preset direction. The adjusting member 26 drives the tray 27 to move a first preset length along the first preset direction, so that the tray 27 moves the target glass slide 8 to a position that matches the other end of the second pipe 10. The glass slide 8 is positioned below the second pipe 10 at a position that matches the other end of the second pipe 10, that is, the glass slide 8 is aligned with the bottom opening of the second pipe 10. The adjusting member 26 can be a two-axis robotic arm.

[0045] As a sample sheet patching device in this embodiment, it may also include a liquid collection tank 16 and a sixth pipe 11. The liquid collection tank 16 is used to store the protective solution 14. One end of the sixth pipe 11 is connected to the output end of the first water pump 1 and the other end is connected to the liquid collection tank 16, so as to transport the protective solution 14 discharged by the first water pump 1 into the liquid collection tank 16.

[0046] As a sample sheet patching device in this embodiment, it may also include a seventh pipe 12, one end of which is connected to the output end of the second water pump 2 and the other end is connected to the liquid collection tank 16, so as to transport the protective solution 14 discharged by the second water pump 2 to the liquid collection tank 16.

[0047] In this embodiment, when the sixth pipe 11 and the seventh pipe 12 are not installed, the protective solution 14 in the first water pump 1 is discharged from the output end of the first water pump 1, and the protective solution 14 in the second water pump 2 is discharged from the output end of the second water pump 2. The sixth pipe 11 can receive the protective solution 14 discharged by the first water pump 1 and transport the protective solution 14 to the collection tank 16; the seventh pipe 12 can receive the protective solution 14 discharged by the second water pump 2 and transport the protective solution 14 to the collection tank 16.

[0048] The detection element 7 may include a photoelectric sensor 28, the line connecting the transmitting end and the receiving end of the photoelectric sensor 28 intersecting the axis of the third pipe 20.

[0049] There can be multiple photoelectric sensors 28.

[0050] In this embodiment of the application, there can be two photoelectric sensors 28, with the line connecting the transmitter and receiver of the first photoelectric sensor 28 and the line connecting the transmitter and receiver of the second photoelectric sensor 28 perpendicular to each other.

[0051] As a sample sheet patching device in this embodiment, it may also include a conveying component for extracting and conveying the protective solution 14 in the collection tank 16 to the sample tank 15.

[0052] In this embodiment, the conveying component can extract the protective solution 14 from the collection tank 16 and convey it to the sample tank 15, thereby improving the utilization rate of the protective solution 14.

[0053] As a sample sheet patching device in this embodiment, it may also include an eighth pipe 19 and a third water pump 3. The two ends of the eighth pipe 19 are respectively connected to the input end of the third water pump 3 and the fourth interface of the second connection structure 6.

[0054] In this embodiment, an eleventh pipe is also included. One end of the eleventh pipe is connected to the output end of the third water pump 3, and the other end is connected to the collection tank 16. The eleventh pipe can receive the protective solution 14 discharged from the third water pump 3 and transport the protective solution 14 into the collection tank 16. After the thin sample 13 in the second pipe 10 falls onto the glass slide 8, the third water pump 3 can discharge the protective solution 14 in the second pipe 10.

[0055] The conveying component may include a ninth pipe 23, a tenth pipe 22 and a fourth water pump 24. The two ends of the ninth pipe 23 are respectively connected to the liquid collection tank 16 and the input end of the fourth water pump 24, and the two ends of the tenth pipe 22 are respectively connected to the output end of the fourth water pump 24 and the sample tank 15.

[0056] In this embodiment, the sample tank 15 is connected to the fourth water pump 24 via the tenth pipe 22, and the fourth water pump 24 is connected to the collection tank 16 via the ninth pipe 23. When the fourth water pump 24 is turned on, the protective solution 14 in the collection tank 16 can be extracted and transported sequentially to the sample tank 15 via the ninth pipe 23 and the tenth pipe 22.

[0057] In this embodiment, one end of the first pipe 9 extends into the sample water tank 15, and the other end is connected to the first connecting structure 25. The first connecting structure 25 is connected to the second connecting structure 6 via the third pipe 20, and is also connected to the second water pump 2 via the fifth pipe 18. The second water pump 2 is also connected to the collection tank 16 via the seventh pipe 12. The second connecting structure 6 is also connected to the first water pump 1 via the fourth pipe 21, and the first water pump 1 is also connected to the collection tank 16 via the sixth pipe 11. One end of the second pipe 10 is connected to the second connecting structure 6, and the other end faces the glass slide 8. The second connecting structure 6 is also connected to the third water pump 3 via the eighth pipe 19. The top of the sample water tank 15 and the top of the collection tank 16 can be open. The tray 27 can be positioned above the collection tank 16. The tray 27 has multiple mounting areas for mounting glass slides 8, and the number of mounting areas can be greater than the number of glass slides 8. When the detection element 7 detects the thin-slice sample 13, the mounting areas on the tray 27 without a glass slide 8 can receive the protective solution 14 discharged from the second pipe 10 at this time. If the second pipe 10 does not contain the thin-slice sample 13, then the mounting areas on the tray 27 will not receive the thin-slice sample 13. The adjusting element 26 is a two-axis robotic arm that can drive the tray 27 to move linearly along a first preset direction, which can be perpendicular to the axis of the second pipe 10.

[0058] In this embodiment, the first connecting structure 25 has a first water guiding cavity, and the first, second, and third interfaces of the first connecting structure 25 are respectively connected to the first water guiding cavity. The second connecting structure 6 has a second water guiding cavity, and the first, second, third, and fourth interfaces of the second connecting structure 6 are respectively connected to the second water guiding cavity. The height of the first interface of the first connecting structure 25 is lower than the height of the second interface of the first connecting structure 25, and the height of the first interface of the second connecting structure 6 is lower than the heights of the second, third, and fourth interfaces of the second connecting structure 6. The first pipe 9, the third pipe 20, the sixth pipe 11, and the seventh pipe 12 are all bendable pipes.

[0059] The following will be combined with the appendix Figure 1-4 This application provides a detailed description of a sample sheet pasting method according to an embodiment. It should be noted that... Figure 1 This is a schematic diagram of the structure of a sample sheet patching device provided in an embodiment of this application. Figure 2 This is a schematic diagram of the structure of a sample slide mounting device provided in this application, in which the adjusting member 26 is connected to the tray 27, the second pipe 10, and the target glass slide 8, respectively. Figure 3 This is a schematic diagram of the structure of a sample sheet patching device provided in this application, in which the detection element 7 is disposed in the third pipe 20. Figure 4 This is a schematic flowchart illustrating a method for attaching a sample sheet according to an embodiment of this application.

[0060] like Figure 4 As shown, the method includes the following steps:

[0061] S1: Open the first valve 4 and the first water pump 1. The first water pump 1 draws the protective solution 14 from the sample water tank 15, so that the protective solution 14 flows sequentially to the first pipe 9, the first connecting structure 25, the third pipe 20, and the second connecting structure 6. The sheet sample 13 flows with the protective solution 14.

[0062] S2: When the thin sheet sample 13 is detected by the test piece 7, the first water pump 1 is turned off, the first valve 4 is closed, and the second valve 5 is opened, so that the protective solution 14 in the second pipe 10 falls down under the action of gravity and is discharged from the second pipe 10.

[0063] S3: Close the second valve 5 and turn on the second water pump 2, so that the protective solution 14 in the third pipe 20 flows into the second connecting structure 6. The protective solution 14 drives the sheet sample 13 to move into the second pipe 10.

[0064] S4: Turn off the second water pump 2 and place the glass slide 8 below the second pipe 10 and at a position that matches the other end of the second pipe 10;

[0065] S5: Open the second valve 5 so that the protective solution 14 and the thin film sample 13 in the second pipe 10 fall onto the glass slide 8 under the action of gravity.

[0066] In this embodiment, all pumps and valves are closed at the start of patch application. A protective solution 14 is stored in the sample tank 15, and the sheet sample 13 is stored in the sample tank 15 and immersed in the protective solution 14. First, the first valve 4 and the first pump 1 are opened. The first pump 1 draws the protective solution 14 from the sample tank 15 into the first pipe 9. Under the action of the first pump 1, the protective solution 14 in the first pipe 9 continues to flow, sequentially flowing to the first connecting structure 25, the third pipe 20, and the second connecting structure 6. As the protective solution 14 flows in the first pipe 9, it carries the sheet sample 13. If the sample 13 moves, it can flow sequentially with the protective solution 14 into the first pipe 9, the first connecting structure 25, the third pipe 20, and the second connecting structure 6. A detection element 7 is provided on the first pipe 9. When the detection element 7 detects the sample 13, it indicates that the sample 13 has moved to a first preset position within the first pipe 9. At this time, the first pipe 9, the first connecting structure 25, the third pipe 20, the second connecting structure 6, and the second pipe 10 all contain the protective solution 14. The second pipe 10 allows liquid to enter from one end and flow out from the other end under gravity; that is, the second pipe is a tubular structure that allows liquid to drain under gravity. Therefore, in the protective solution... When liquid 14 and / or sheet sample 13 enter the interior of the second pipe 10 from one end, the protective solution 14 and / or sheet sample 13 can flow out from the other end of the second pipe 10 under the action of gravity. Then, the first water pump 1 is turned off, the first valve 4 is turned off, and the second valve 5 is turned on. The protective solution 14 in the second pipe 10 falls and is discharged from the second pipe 10 under the action of gravity. Then, the second valve 5 is turned off and the second water pump 2 is turned on, so that the protective solution 14 in the third pipe 20 flows into the second connecting structure 6. The protective solution 14 carries the sheet sample 13 to move into the second pipe 10. Through the pressurization of the second water pump 2, the liquid column and the sheet sample are pushed. 13. Compressing the air in the second pipe 10 allows the protective solution 14 and the thin film sample 13 to enter the second connecting structure 6. The protective solution 14 moves the thin film sample 13 into the second pipe 10. Then, the second water pump 2 is turned off, and the glass slide 8 is placed below the second pipe 10 and at a position matching the other end of the second pipe 10. During this period, the thin film sample 13 in the second pipe 10 settles and expands, reducing the probability of the thin film sample 13 folding or curling due to pressure movement in the protective solution 14. Finally, the second valve 5 is opened, allowing the protective solution 14 and the thin film sample 13 in the second pipe 10 to fall onto the glass slide 8 under the action of gravity.

[0067] In this embodiment of the application, when the adjusting member 26 is connected to the tray 27 and multiple glass slides 8 are placed on the tray 27, step S2 specifically includes: the adjusting member 26 drives the tray 27 to move by a second preset length, so that the first part of the tray 27 without glass slides 8 is moved to a position below the second pipe 10 that matches the other end of the second pipe 10, that is, the first part of the tray 27 is aligned with the bottom opening of the second pipe 10. When the detection member 7 detects the thin film sample 13, the first water pump 1 is turned off, the first valve 4 is closed, and the second valve 5 is opened, so that the protective solution 14 in the second pipe 10 falls down under the action of gravity and is discharged from the first pipe 9. The protective solution 14 falls onto the first part of the tray 27.

[0068] In this embodiment of the application, when the adjusting member 26 is connected to the tray 27 and multiple glass slides 8 are placed on the tray 27, step S4 specifically includes: turning off the second water pump 2 and the first valve 4, and the adjusting member 26 driving the tray 27 to move a third preset length, so that the target glass slide 8 on the tray 27 moves to a position below the second pipe 10 that matches the other end of the second pipe 10, that is, aligning the target glass slide 8 with the bottom opening of the second pipe 10.

[0069] In this embodiment, when one end of the sixth pipe 11 is connected to the output end of the first water pump 1 and the other end is connected to the collection tank 16, the sixth pipe 11 transports the protective solution 14 discharged by the first water pump 1 to the collection tank 16. When one end of the seventh pipe 12 is connected to the output end of the second water pump 2 and the other end is connected to the collection tank 16, the seventh pipe 12 transports the protective solution 14 discharged by the second water pump 2 to the collection tank 16.

[0070] In this embodiment of the application, after step S5, the method further includes: turning on the third water pump 3 so that the third water pump 3 empties the protective solution 14 in the second pipe 10; turning on the fourth water pump 24 so that the fourth water pump 24 extracts the protective solution 14 in the collection tank 16 and transports the protective solution 14 to the sample tank 15 through the ninth pipe 23 and the tenth pipe 22.

[0071] Those skilled in the art will clearly understand that the technical solutions of the embodiments of this application can be implemented by means of software and / or hardware. In this specification, "unit," "module," and "section" refer to software and / or hardware capable of independently completing or cooperating with other components to complete a specific function. Hardware may include, for example, a Field-Programmable Gate Array (FPGA), an Integrated Circuit (IC), etc.

[0072] It should be noted that, for the sake of simplicity, the foregoing method embodiments are all described as a series of actions. However, those skilled in the art should understand that this application is not limited to the described order of actions, as some steps may be performed in other orders or simultaneously according to this application. Furthermore, those skilled in the art should also understand that the embodiments described in the specification are preferred embodiments, and the actions and modules involved are not necessarily essential to this application.

[0073] In the above embodiments, the descriptions of each embodiment have different focuses. For parts not described in detail in a certain embodiment, please refer to the relevant descriptions in other embodiments.

[0074] In the several embodiments provided in this application, it should be understood that the disclosed apparatus can be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative; for instance, the division of units is only a logical functional division, and in actual implementation, there may be other division methods. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Furthermore, the coupling or direct coupling or communication connection shown or discussed may be through some service interface; the indirect coupling or communication connection between devices or units may be electrical or other forms.

[0075] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment according to actual needs.

[0076] Furthermore, the functional units in the various embodiments of this application can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. The integrated unit can be implemented in hardware or as a software functional unit.

[0077] If the integrated unit is implemented as a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage device (CMD). Based on this understanding, the technical solution of this application, in essence, or the part that contributes to the prior art, or all or part of the technical solution, can be embodied in the form of a software product. This computer software product is stored in a memory and includes several instructions to enable a computer device, such as a personal computer, server, or network device, to execute all or part of the steps of the methods described in the various embodiments of this application. The aforementioned memory includes various media capable of storing program code, such as USB flash drives, read-only memory (ROM), random access memory (RAM), portable hard drives, magnetic disks, or optical disks.

[0078] Those skilled in the art will understand that all or part of the steps in the various methods of the above embodiments can be implemented by a program instructing related hardware. The program can be stored in a computer-readable storage medium, which may include: flash drive, read-only memory, ROM, random access memory, RAM, disk or optical disk, etc.

[0079] The foregoing description is merely an exemplary embodiment of this disclosure and should not be construed as limiting the scope of this disclosure. Any equivalent changes and modifications made in accordance with the teachings of this disclosure shall still fall within the scope of this disclosure. Those skilled in the art will readily conceive of embodiments of this disclosure upon considering the specification and practicing the disclosure herein. This application is intended to cover any variations, uses, or adaptations of this disclosure that follow the general principles of this disclosure and include common knowledge or customary techniques in the art not described herein. The specification and embodiments are to be considered exemplary only, and the scope and spirit of this disclosure are defined by the claims.

Claims

1. A sample sheet mounting device, characterized in that: The system includes a sample tank (15), a first connecting structure (25), a second connecting structure (6), a first pipe (9), a second pipe (10), a third pipe (20), a fourth pipe (21), a fifth pipe (18), a first valve (4), a second valve (5), a test piece (7), a glass slide (8), a first water pump (1), and a second water pump (2). The first connecting structure (25) and the second connecting structure (6) are both structures for transporting liquids. The first connecting structure (25) and the second connecting structure (6) each include multiple interfaces connected to their interiors. The second pipe (10) allows liquid to enter from one end and flow out from the other end under gravity. The sample tank (15) is used to store a protective solution (14) and a thin-film sample (13) respectively. The first valve (4) is located on the first pipe (9), and the second valve (5) is located on the second pipe (18). 0) On; one end of the first pipe (9) extends into the protective solution (14) of the sample water tank (15), and the other end is connected to the first interface of the first connecting structure (25); one end of the second pipe (10) is connected to the first interface of the second connecting structure (6); the glass slide (8) is set below the second pipe (10) and at a position matching the other end of the second pipe (10); the two ends of the third pipe (20) are respectively connected to the second interface of the first connecting structure (25) and the second interface of the second connecting structure (6); the detection element (7) is set on the third pipe (20) for detecting the thin section sample (13); the two ends of the fourth pipe (21) are respectively connected to the third interface of the second connecting structure (6) and the input end of the first water pump (1); the two ends of the fifth pipe (18) are respectively connected to the third interface of the first connecting structure (25) and the input end of the second water pump (2).

2. The sample sheet mounting device as described in claim 1, characterized in that: The third pipe (20) is inclined upward along the direction from one end connected to the first connecting structure (25) to the other end connected to the second connecting structure (6), and / or the second interface of the second connecting structure (6) is inclined upward along the direction from one end connected to the third connecting structure (20) to the center of the second interface of the second connecting structure (6).

3. A sample sheet mounting device as described in claim 1 or 2, characterized in that: The first water pump (1) is a diaphragm pump.

4. A sample sheet mounting device as described in claim 1 or 2, characterized in that: Along the direction of gravity, the second interface of the second connecting structure (6) is lower than the first interface of the second connecting structure (6).

5. A sample sheet mounting device as described in claim 1 or 2, characterized in that: It also includes a tray (27) and an adjusting member (26), wherein the glass slides (8) are multiple ones respectively disposed on the tray (27); the adjusting member (26) is connected to the tray (27) and is used to drive the tray (27) to move, so that the tray (27) drives the target glass slide (8) to a position that matches the other end of the second pipe (10).

6. A sample sheet mounting device as described in claim 1 or 2, characterized in that: It also includes a collection tank (16) and a sixth pipe (11). The collection tank (16) is used to store the protective solution (14). One end of the sixth pipe (11) is connected to the output end of the first water pump (1) and the other end is connected to the collection tank (16) to transport the protective solution (14) discharged by the first water pump (1) into the collection tank (16).

7. The sample sheet mounting device as described in claim 6, characterized in that: It also includes a seventh pipe (12), one end of which is connected to the output end of the second water pump (2) and the other end is connected to the liquid collection tank (16) to transport the protective solution (14) discharged by the second water pump (2) into the liquid collection tank (16).

8. A sample sheet mounting device as described in claim 1 or 2, characterized in that: The detection component (7) includes a photoelectric sensor (28), and the line connecting the transmitting end and the receiving end of the photoelectric sensor (28) intersects the axis of the third pipe (20).

9. The sample sheet mounting device as described in claim 8, characterized in that: There are multiple photoelectric sensors (28).

10. The sample sheet mounting device as described in claim 6, characterized in that: It also includes a conveyor for extracting and conveying the protective solution (14) in the collection tank (16) to the sample tank (15).

11. A sample sheet mounting device as described in claim 1 or 2, characterized in that: It also includes an eighth pipe (19) and a third water pump (3), the two ends of which are connected to the input end of the third water pump (3) and the fourth interface of the second connection structure (6), respectively.

12. The sample sheet mounting device as described in claim 10, characterized in that, The conveying components include a ninth pipe (23), a tenth pipe (22) and a fourth water pump (24). The two ends of the ninth pipe (23) are connected to the liquid collection tank (16) and the input end of the fourth water pump (24) respectively. The two ends of the tenth pipe (22) are connected to the output end of the fourth water pump (24) and the sample tank (15) respectively.

13. A method applied to a sample sheet mounting apparatus as described in any one of claims 1 to 12, characterized in that, Includes the following steps: S1: Open the first valve (4) and the first water pump (1). The first water pump (1) draws the protective solution (14) in the sample water tank (15) so that the protective solution (14) flows sequentially to the first pipe (9), the first connecting structure (25), the third pipe (20), and the second connecting structure (6). The sheet sample (13) flows with the protective solution (14). S2: When the test piece (7) detects the sheet sample (13), the first water pump (1) is turned off, the first valve (4) is turned off, and the second valve (5) is turned on, so that the protective solution (14) in the second pipe (10) falls down under the action of gravity and is discharged out of the second pipe (10); S3: Close the second valve (5) and turn on the second water pump (2) so that the protective solution (14) in the third pipe (20) flows into the second connecting structure (6), and the protective solution (14) drives the sheet sample (13) to move into the second pipe (10); S4: Turn off the second water pump (2) and place the glass slide (8) below the second pipe (10) and at the position that matches the other end of the second pipe (10); S5: Open the second valve (5) so that the protective solution (14) and the thin film sample (13) in the second pipe (10) fall onto the glass slide (8) under the action of gravity.

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